Rorug06G0456000

SPIRAL1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003119
Physical Location & Seq
Reverse (-)
58376175 .. 58381677
5503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0456000.1

Sequence Viewer

Length: 2970 bp
ATGGCCACGACGAGCATGGCGGTTAGGGCTTTTCTTCTTCTTCTACTGGTATTCCTATCTCTCGCAAATCTCACTCTTTCGCTTTACGAAGATCAAGTCGGCCTCGTCGATTGGCACCAACAATATATAGGGAAAGTGAAGGACGCAGTGTTTCATACTCAAAAGAGTGGGCGAAAGCGTGTTGTGGTGTCCACTGAAGAGAATGTTATAGCATCACTTGATCTCCGACACGGGGAGATATTTTGGAGACATGTTCTCGGGAGCAATGATGTTGTCGATGGAATTGACATAGCATTGGGAAAATATGTCATTACCCTTTCGTCTGAAGGAAGTACTTTGAGAGCATGGAACCTTCCAGATGGTCAGATGGTGTGGCAATCTTTTATAGATGGCTCAGGGGCCTCAAAATCATTATTAACTGTCCCGACAAATTTGATCGTCAACAAGGAAAATTTGATCCTTGTTTTTGGTAAAGGGTCTCTACATGCTGTTTCTGGCATTGATGGTGAGGTTCTTTGGACAAAGGATTTTGCAGCTGAAAGTCTAGAGGTTCAACATATTATTCAACCTGTTGGAAGTGAGGCAATCTATGTACTAGGATTTGTTGGTTCTTCCCAGTTTGATGCATATCAGATCAATCCTAAGAACGGAGAGATACTGAAGCACAATAGTGCAGCACTTTCTGGCGGCTATTCTGGGGAAGCAATATTGGCTTCGAGTGACATTCTTGTGACATTGGATGCCTCTAGGTCAAAGTTGGTAGTTATAAGCTTTCAGGATGGTGAACTTAACTTGCAACAGACATCCATATCTGATATATTGGGGGATTCTTCTGGAACACCAGTACTGTTATCTTCAAAACTACCAGGAATGTTTTCCGTAAAGCTTGATGGAGGAGTGACACTCATAAAAGTGACTGTAGAAGCCAAGTTGGAGGTGATGGATAAAATAAGTAGTGTGGTAGCTATTAGTGATGCAATTCCCCTTACTGAAGGTCAACAAGCTTTCGCACTAGTTCAACATGGAGATGGTAAGATTCATCTTACAGTGAAGCTTAGTCACGATCTGAGTGGTGATTTGCTGAAGGAGAGTATAGTTATGGACAATCAGAGGGGGATGGTGCATAAGGTTTTTATAAACAGTTATATCCGGACAGACAGATCAAATGGGTTTAGGGCCTTGATTGTCATGGAGGATCATTCACTTTTGTTGTTACAACAAGGTGCCATTGTCTGGAGTAGGGAGGATGGTCTTGCCTCAATTGTTGATGTACTAACATCAGAACTACCCGTGGAAAAGGAAGGTGTATCTGTAGCAAAAGTTGAGGAAAACCTTTTTGAGTGGCTTAAGGGACACCTACTGAAGCTTAAGGGAACCTTAATGCTTGCAAGTGCTGATGATGTTGCTGCTATACAAGAGAGGAGGTTAAAAAGTTCTGAAAAGAGCAAACTGACCCGAGACCATAATGGTTTTCGGAAGCTTCTCATAGTACTTACGAGAGCGGGAAAGCTCTTTGCCTTGCACACTGGATATGGACAAGTTGTCTGGTCTCTGTTACTGCCTAATCTGCGTAAATCTGAGTGCGAGTTTCCGACTGGTTTGAATATTTATCAGTGGCAGGTACCCCATCATCACGCAATGGATGAAGATCCATCTATCCTCATAGTAGGTCGATGTGGGCAAAGTTCTGATGCACCAGGTGTTCTGTCTATTGTTGATGCATATACTGGCACTGAAATTAATTCAATGGATCTCGTACATTCCATTTCGCAAGTTATTCCACTGCCATTTACTGATTCAACAGAACAGCGACTTCATCTACTAATAGATGGAAACCAGCATGCATATCTATATCCAAGAACATCCGAGGCCATTGATATCTTTCAACGCGAGTTCTCAAACATATACTGGTACTCTGTTGAGACTAAGAATGGCATTATCAAAGGACATGTCTTGAAGAGCAATTGCATCCAGGAAGTTGTAGACAACTATTGCTTCGAGTCCAGGGATATATGGTCAATTGTATTCCCATCTGACTCAGAAAAGATCATTGCAACAGTGACAAGAAAACCAAATGAGGTGGTTCACACTCAAGCAAAGGTTATAGCTGCTGAAGAAGATCTGATGTATAAGTATATATCTAAGAATCTACTCTTTGTTGCAACTGTTGCACCTAAAGGCAGTGGTGCAATTGGAACAGCTACCCCAGAGGAGTCGTGGTTGACTGTATATCTTATCGATACTGTAACTGGTCGTATATTGCATCGGATGACCCATCATGGTGCACAGGGACCTGTCCACGCTGTTTTCAGTGAGAATTGGGTTGTATACCACTACTTTAATCTTAGAGCACACAGATATGAGATGTCAGTCATAGAGATATATGATCAGTCCCGTGCGGACAATAAGGATGTTTGGAAGCTGATTCTTGGGAAACATAATCTCACTTCACCTATTTCTTCATATTCCCGACCAGAGGTTGTGACGAAATCACAGTCTTACTTTTTCACGTATTCTGTAAAAGCAATAGATGTGACATTGACAGCAAAGGGTATTACTTCAAAGCAGCTTCTTATTGGTACAATTGGGGATCAGGTTTTGGCACTTGACAAGCGATTTTTGGATCCCCGTCGATCAGTCAACCCCACACCAGCTGAGAAAGAGGAAGGCATTATACCTCTAACAGATTCATTGCCAATAATTCCTCAGTCCTATGTGACACATGCTCTAAGAGTCGAAGGACTCCGAGGCATAGTGACAGTACCTGCCAAGCTGGAGTCAACAACCCTTGTCTTCGTATATGGAGTGGATCTGTTTTTTACTCAACTCGCACCGTCAAGGACCTACGATTCGCTGACTGATGATTTCAGCTATGCATTGCTTCTCATCACCATCGTTGCACTCATAGCAGCAATCTTTGTAACCTGGATTTTATCTGAGAAAAAAGAACTACGAGAGAAGTGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

989

Amino Acids

109.54

Weight (kDa)

5.92

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EMC1_N PF25293 28 - 416 6.8e-136 EMC1 N-terminal beta-propeller domain
PQQ_2 PF13360 56 - 261 7.4e-10 Outer membrane protein assembly factor BamB
Beta-prop_ACSF4 PF13570 57 - 179 3.8e-06 Beta-alanine-activating enzyme, beta-propeller
PQQ_2 PF13360 112 - 286 3.4e-06 Outer membrane protein assembly factor BamB
EMC1_C PF07774 771 - 988 2.7e-68 ER membrane protein complex subunit 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 767, 1136
Acc36I ACCTGC 2 cut(s) 1609, 2773
Acc65I GGTACC 1 cut(s) 1621
AccB1I GGYRCC 3 cut(s) 114, 1223, 1621
AccB7I CCANNNNNTGG 1 cut(s) 1233
AccBSI CCGCTC 1 cut(s) 1502
AccI GTMKAC 2 cut(s) 1983, 2328
AccII CGCG 1 cut(s) 1890
AccIII TCCGGA 1 cut(s) 1149
AciI CCGC 4 cut(s) 20, 687, 1502, 2399
AclWI GGATC 8 cut(s) 451, 1203, 1643, 1758, 2598, 2618, 2631, 2817
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 430, 451
AcuI CTGAAG 7 cut(s) 216, 345, 680, 1011, 1103, 1382, 2133
AdeI CACNNNGTG 1 cut(s) 1700
AfiI CCNNNNNNNGG 4 cut(s) 232, 647, 1233, 2476
AflII CTTAAG 2 cut(s) 1346, 1367
AflIII ACRYGT 2 cut(s) 250, 1948
AhdI GACNNNNNGTC 1 cut(s) 1541
AhlI ACTAGT 1 cut(s) 1012
AjnI CCWGG 5 cut(s) 865, 1696, 1971, 2003, 2924
AleI CACNNNNGTG 1 cut(s) 669
Alw21I GWGCWC 2 cut(s) 2287, 2353
Alw26I GTCTC 5 cut(s) 241, 483, 1452, 1554, 1916
Alw44I GTGCAC 1 cut(s) 2283
AlwI GGATC 8 cut(s) 451, 1203, 1643, 1758, 2598, 2618, 2631, 2817
AlwNI CAGNNNCTG 1 cut(s) 2765
Ama87I CYCGRG 2 cut(s) 257, 1455
Aor13HI TCCGGA 1 cut(s) 1149
AoxI GGCC 5 cut(s) 3, 100, 399, 1176, 1869
ApaLI GTGCAC 1 cut(s) 2283
ApeKI GCWGC 6 cut(s) 533, 674, 1406, 2108, 2566, 2909
ApoI RAATTY 2 cut(s) 430, 451
ArsI GACNNNNNNTTYG 8 cut(s) 81, 113, 1935, 1967, 2480, 2512, 2833, 2865
AseI ATTAAT 1 cut(s) 1740
Asp700I GAANNNNTTC 1 cut(s) 874
Asp718I GGTACC 1 cut(s) 1621
AspS9I GGNCC 4 cut(s) 399, 1176, 2291, 2841
AsuHPI GGTGA 6 cut(s) 518, 794, 949, 1085, 2442, 2881
AvaI CYCGRG 2 cut(s) 257, 1455
AvaII GGWCC 2 cut(s) 2291, 2841
BaeGI GKGCMC 1 cut(s) 2287
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 2623
BanI GGYRCC 3 cut(s) 114, 1223, 1621
BarI GAAGNNNNNNTAC 2 cut(s) 2658, 2690
BbsI GAAGAC 1 cut(s) 2785
Bbv12I GWGCWC 2 cut(s) 2287, 2353
BbvI GCAGC 6 cut(s) 545, 686, 1393, 2095, 2578, 2921
BciT130I CCWGG 5 cut(s) 867, 1698, 1973, 2005, 2926
BclI TGATCA 1 cut(s) 2386
BcoDI GTCTC 5 cut(s) 241, 483, 1452, 1554, 1916
BcuI ACTAGT 1 cut(s) 1012
BfaI CTAG 4 cut(s) 545, 596, 747, 1013
BfmI CTRYAG 2 cut(s) 918, 1311
BfrI CTTAAG 2 cut(s) 1346, 1367
BfuAI ACCTGC 2 cut(s) 1609, 2773
BglII AGATCT 1 cut(s) 2119
BisI GCNGC 7 cut(s) 534, 675, 688, 1407, 2109, 2567, 2910
BlsI GCNGC 7 cut(s) 535, 676, 689, 1408, 2110, 2568, 2911
BmcAI AGTACT 3 cut(s) 334, 846, 1491
Bme1390I CCNGG 5 cut(s) 867, 1698, 1973, 2005, 2926
Bme18I GGWCC 2 cut(s) 2291, 2841
BmeRI GACNNNNNGTC 1 cut(s) 1541
BmeT110I CYCGRG 2 cut(s) 257, 1455
BmgT120I GGNCC 4 cut(s) 399, 1176, 2291, 2841
BmiI GGNNCC 8 cut(s) 116, 350, 400, 1225, 1375, 1623, 2292, 2625
BmrFI CCNGG 5 cut(s) 867, 1698, 1973, 2005, 2926
BmrI ACTGGG 1 cut(s) 610
BmsI GCATC 8 cut(s) 221, 613, 730, 964, 1681, 1708, 1977, 2272
BmuI ACTGGG 1 cut(s) 610
BpiI GAAGAC 1 cut(s) 2785
BplI GAGNNNNNCTC 2 cut(s) 888, 920
BpmI CTGGAG 2 cut(s) 1255, 2795
Bpu10I CCTNAGC 1 cut(s) 394
BpuEI CTTGAG 1 cut(s) 2076
Bsa29I ATCGAT 1 cut(s) 2238
BsaAI YACGTR 1 cut(s) 2511
BsaBI GATNNNNATC 2 cut(s) 627, 1647
BsaI GGTCTC 3 cut(s) 483, 1452, 1554
BsaJI CCNNGG 4 cut(s) 1290, 1866, 2004, 2746
BsaWI WCCGGW 1 cut(s) 1149
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 2204, 2234
Bsc4I CCNNNNNNNGG 4 cut(s) 232, 647, 1233, 2476
Bse1I ACTGG 8 cut(s) 51, 616, 842, 1531, 1600, 1732, 1913, 2254
Bse3DI GCAATG 5 cut(s) 271, 1644, 2049, 2690, 2876
Bse8I GATNNNNATC 2 cut(s) 627, 1647
BseAI TCCGGA 1 cut(s) 1149
BseBI CCWGG 5 cut(s) 867, 1698, 1973, 2005, 2926
BseCI ATCGAT 1 cut(s) 2238
BseDI CCNNGG 4 cut(s) 1290, 1866, 2004, 2746
BseJI GATNNNNATC 2 cut(s) 627, 1647
BseLI CCNNNNNNNGG 4 cut(s) 232, 647, 1233, 2476
BseMI GCAATG 5 cut(s) 271, 1644, 2049, 2690, 2876
BseMII CTCAG 7 cut(s) 408, 1058, 1569, 2052, 2646, 2720, 2928
BseNI ACTGG 8 cut(s) 51, 616, 842, 1531, 1600, 1732, 1913, 2254
BseRI GAGGAG 3 cut(s) 909, 1435, 2225
BseSI GKGCMC 1 cut(s) 2287
BseXI GCAGC 6 cut(s) 545, 686, 1393, 2095, 2578, 2921
BsgI GTGCAG 1 cut(s) 693
Bsh1236I CGCG 1 cut(s) 1890
BshFI GGCC 5 cut(s) 5, 102, 401, 1178, 1871
BshNI GGYRCC 3 cut(s) 114, 1223, 1621
BshVI ATCGAT 1 cut(s) 2238
BsiHKAI GWGCWC 2 cut(s) 2287, 2353
BsiHKCI CYCGRG 2 cut(s) 257, 1455
BsiSI CCGG 1 cut(s) 1150
BslFI GGGAC 4 cut(s) 407, 1365, 2304, 2377
BslI CCNNNNNNNGG 4 cut(s) 232, 647, 1233, 2476
BsmAI GTCTC 5 cut(s) 241, 483, 1452, 1554, 1916
BsmFI GGGAC 4 cut(s) 407, 1365, 2304, 2377
BsnI GGCC 5 cut(s) 5, 102, 401, 1178, 1871
Bso31I GGTCTC 3 cut(s) 483, 1452, 1554
BsoBI CYCGRG 2 cut(s) 257, 1455
Bsp1286I GDGCHC 2 cut(s) 2287, 2353
Bsp13I TCCGGA 1 cut(s) 1149
BspACI CCGC 4 cut(s) 20, 687, 1502, 2399
BspANI GGCC 5 cut(s) 5, 102, 401, 1178, 1871
BspCNI CTCAG 7 cut(s) 407, 1059, 1570, 2051, 2647, 2719, 2929
BspDI ATCGAT 1 cut(s) 2238
BspEI TCCGGA 1 cut(s) 1149
BspFNI CGCG 1 cut(s) 1890
BspLI GGNNCC 8 cut(s) 116, 350, 400, 1225, 1375, 1623, 2292, 2625
BspMI ACCTGC 2 cut(s) 1609, 2773
BspPI GGATC 8 cut(s) 451, 1203, 1643, 1758, 2598, 2618, 2631, 2817
BspQI GCTCTTC 1 cut(s) 1952
BspT107I GGYRCC 3 cut(s) 114, 1223, 1621
BspTI CTTAAG 2 cut(s) 1346, 1367
BspTNI GGTCTC 3 cut(s) 483, 1452, 1554
BsrBI CCGCTC 1 cut(s) 1502
BsrDI GCAATG 5 cut(s) 271, 1644, 2049, 2690, 2876
BsrI ACTGG 8 cut(s) 51, 616, 842, 1531, 1600, 1732, 1913, 2254
BssECI CCNNGG 4 cut(s) 1290, 1866, 2004, 2746
BssNAI GTATAC 1 cut(s) 2329
Bst1107I GTATAC 1 cut(s) 2329
Bst2UI CCWGG 5 cut(s) 867, 1698, 1973, 2005, 2926
Bst6I CTCTTC 2 cut(s) 192, 1952
BstAFI CTTAAG 2 cut(s) 1346, 1367
BstAPI GCANNNNNTGC 1 cut(s) 2168
BstBAI YACGTR 1 cut(s) 2511
BstC8I GCNNGC 2 cut(s) 1386, 1842
BstDSI CCRYGG 1 cut(s) 1290
BstFNI CGCG 1 cut(s) 1890
BstMAI GTCTC 5 cut(s) 241, 483, 1452, 1554, 1916
BstMWI GCNNNNNNNGC 5 cut(s) 26, 710, 1567, 2168, 2906
BstNI CCWGG 5 cut(s) 867, 1698, 1973, 2005, 2926
BstNSI RCATGY 5 cut(s) 254, 488, 1844, 1952, 2726
BstSCI CCNGG 5 cut(s) 865, 1696, 1971, 2003, 2924
BstSFI CTRYAG 2 cut(s) 918, 1311
BstSLI GKGCMC 1 cut(s) 2287
BstUI CGCG 1 cut(s) 1890
BstV1I GCAGC 6 cut(s) 545, 686, 1393, 2095, 2578, 2921
BstV2I GAAGAC 1 cut(s) 2785
BstX2I RGATCY 5 cut(s) 1648, 1750, 2119, 2623, 2809
BstYI RGATCY 5 cut(s) 1648, 1750, 2119, 2623, 2809
BstZ17I GTATAC 1 cut(s) 2329
Bsu15I ATCGAT 1 cut(s) 2238
BsuRI GGCC 5 cut(s) 5, 102, 401, 1178, 1871
BsuTUI ATCGAT 1 cut(s) 2238
BtgI CCRYGG 1 cut(s) 1290
BtsI GCAGTG 3 cut(s) 153, 1781, 2188
BveI ACCTGC 2 cut(s) 1609, 2773
Cac8I GCNNGC 2 cut(s) 1386, 1842
CaiI CAGNNNCTG 1 cut(s) 2765
Cfr13I GGNCC 4 cut(s) 399, 1176, 2291, 2841
ClaI ATCGAT 1 cut(s) 2238
CseI GACGC 1 cut(s) 152
CsiI ACCWGGT 1 cut(s) 1696
CspCI CAANNNNNGTGG 2 cut(s) 2061, 2096
DraIII CACNNNGTG 1 cut(s) 1700
DriI GACNNNNNGTC 1 cut(s) 1541
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 192, 1952
Eam1105I GACNNNNNGTC 1 cut(s) 1541
EarI CTCTTC 2 cut(s) 192, 1952
Eco31I GGTCTC 3 cut(s) 483, 1452, 1554
Eco32I GATATC 1 cut(s) 1879
Eco47I GGWCC 2 cut(s) 2291, 2841
Eco57I CTGAAG 7 cut(s) 216, 345, 680, 1011, 1103, 1382, 2133
Eco88I CYCGRG 2 cut(s) 257, 1455
EcoO109I RGGNCCY 4 cut(s) 399, 1176, 2291, 2841
EcoRII CCWGG 5 cut(s) 865, 1696, 1971, 2003, 2924
EcoRV GATATC 1 cut(s) 1879
EcoT22I ATGCAT 4 cut(s) 628, 1723, 1846, 2878
FalI AAGNNNNNCTT 2 cut(s) 1361, 1393
FaqI GGGAC 4 cut(s) 407, 1365, 2304, 2377
FauI CCCGC 1 cut(s) 1495
FbaI TGATCA 1 cut(s) 2386
FblI GTMKAC 2 cut(s) 1983, 2328
Fnu4HI GCNGC 7 cut(s) 534, 675, 688, 1407, 2109, 2567, 2910
Fsp4HI GCNGC 7 cut(s) 534, 675, 688, 1407, 2109, 2567, 2910
FspBI CTAG 4 cut(s) 545, 596, 747, 1013
GluI GCNGC 7 cut(s) 534, 675, 688, 1407, 2109, 2567, 2910
GsuI CTGGAG 2 cut(s) 1255, 2795
HaeIII GGCC 5 cut(s) 5, 102, 401, 1178, 1871
HapII CCGG 1 cut(s) 1150
HgaI GACGC 1 cut(s) 152
HincII GTYRAC 5 cut(s) 442, 998, 2223, 2641, 2781
HindII GTYRAC 5 cut(s) 442, 998, 2223, 2641, 2781
HindIII AAGCTT 6 cut(s) 769, 884, 1002, 1052, 1364, 1478
HpaII CCGG 1 cut(s) 1150
HphI GGTGA 6 cut(s) 518, 794, 949, 1085, 2442, 2881
Hpy99I CGWCG 3 cut(s) 13, 110, 2634
HpyAV CCTTC 8 cut(s) 133, 320, 362, 986, 1078, 1295, 2660, 2732
HpyCH4IV ACGT 1 cut(s) 2510
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 710, 1567, 2168, 2906
HpySE526I ACGT 1 cut(s) 2510
Kpn2I TCCGGA 1 cut(s) 1149
KpnI GGTACC 1 cut(s) 1625
Ksp22I TGATCA 1 cut(s) 2386
LguI GCTCTTC 1 cut(s) 1952
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 6 cut(s) 545, 686, 1393, 2095, 2578, 2921
LweI GCATC 8 cut(s) 221, 613, 730, 964, 1681, 1708, 1977, 2272
MabI ACCWGGT 1 cut(s) 1696
MaeI CTAG 4 cut(s) 545, 596, 747, 1013
MaeII ACGT 1 cut(s) 2510
MbiI CCGCTC 1 cut(s) 1502
MfeI CAATTG 5 cut(s) 1260, 1963, 2019, 2190, 2583
MflI RGATCY 5 cut(s) 1648, 1750, 2119, 2623, 2809
MhlI GDGCHC 2 cut(s) 2287, 2353
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 6 cut(s) 2009, 2030, 2222, 2736, 2742, 2786
MmeI TCCRAC 4 cut(s) 250, 553, 912, 1616
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 4 cut(s) 628, 1723, 1846, 2878
MroI TCCGGA 1 cut(s) 1149
MroXI GAANNNNTTC 1 cut(s) 874
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 416, 789, 1347, 1368, 1379, 1427, 1740, 2340
MslI CAYNNNNRTG 6 cut(s) 669, 728, 911, 1026, 2280, 2358
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 536, 2654
MspCI CTTAAG 2 cut(s) 1346, 1367
MspI CCGG 1 cut(s) 1150
MspR9I CCNGG 5 cut(s) 867, 1698, 1973, 2005, 2926
MunI CAATTG 5 cut(s) 1260, 1963, 2019, 2190, 2583
MvaI CCWGG 5 cut(s) 867, 1698, 1973, 2005, 2926
MvnI CGCG 1 cut(s) 1890
MwoI GCNNNNNNNGC 5 cut(s) 26, 710, 1567, 2168, 2906
NlaIV GGNNCC 8 cut(s) 116, 350, 400, 1225, 1375, 1623, 2292, 2625
NsiI ATGCAT 4 cut(s) 628, 1723, 1846, 2878
NspI RCATGY 5 cut(s) 254, 488, 1844, 1952, 2726
OliI CACNNNNGTG 1 cut(s) 669
PaeI GCATGC 1 cut(s) 1844
PciI ACATGT 2 cut(s) 250, 1948
PciSI GCTCTTC 1 cut(s) 1952
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 874
PfeI GAWTC 7 cut(s) 827, 1036, 1796, 2146, 2425, 2687, 2849
PflMI CCANNNNNTGG 1 cut(s) 1233
PfoI TCCNGGA 1 cut(s) 1971
PkrI GCNGC 7 cut(s) 535, 676, 689, 1408, 2110, 2568, 2911
PleI GAGTC 6 cut(s) 2008, 2030, 2221, 2736, 2741, 2785
PpsI GAGTC 6 cut(s) 2008, 2030, 2221, 2736, 2741, 2785
Ppu21I YACGTR 1 cut(s) 2511
PpuMI RGGWCCY 2 cut(s) 2291, 2841
PscI ACATGT 2 cut(s) 250, 1948
PshBI ATTAAT 1 cut(s) 1740
PsiI TTATAA 2 cut(s) 767, 1136
Psp5II RGGWCCY 2 cut(s) 2291, 2841
Psp6I CCWGG 5 cut(s) 865, 1696, 1971, 2003, 2924
PspGI CCWGG 5 cut(s) 865, 1696, 1971, 2003, 2924
PspN4I GGNNCC 8 cut(s) 116, 350, 400, 1225, 1375, 1623, 2292, 2625
PspPI GGNCC 4 cut(s) 399, 1176, 2291, 2841
PspPPI RGGWCCY 2 cut(s) 2291, 2841
PstNI CAGNNNCTG 1 cut(s) 2765
PsuI RGATCY 5 cut(s) 1648, 1750, 2119, 2623, 2809
PvuII CAGCTG 2 cut(s) 536, 2654
RseI CAYNNNNRTG 6 cut(s) 669, 728, 911, 1026, 2280, 2358
SapI GCTCTTC 1 cut(s) 1952
SaqAI TTAA 8 cut(s) 416, 789, 1347, 1368, 1379, 1427, 1740, 2340
SatI GCNGC 7 cut(s) 534, 675, 688, 1407, 2109, 2567, 2910
Sau96I GGNCC 4 cut(s) 399, 1176, 2291, 2841
ScaI AGTACT 3 cut(s) 334, 846, 1491
SchI GAGTC 6 cut(s) 2009, 2030, 2222, 2736, 2742, 2786
ScrFI CCNGG 5 cut(s) 867, 1698, 1973, 2005, 2926
SduI GDGCHC 2 cut(s) 2287, 2353
SexAI ACCWGGT 1 cut(s) 1696
SfaNI GCATC 8 cut(s) 221, 613, 730, 964, 1681, 1708, 1977, 2272
SfcI CTRYAG 2 cut(s) 918, 1311
SinI GGWCC 2 cut(s) 2291, 2841
SmiMI CAYNNNNRTG 6 cut(s) 669, 728, 911, 1026, 2280, 2358
SmlI CTYRAG 3 cut(s) 1346, 1367, 2091
SmoI CTYRAG 3 cut(s) 1346, 1367, 2091
SpeI ACTAGT 1 cut(s) 1012
SphI GCATGC 1 cut(s) 1844
SsiI CCGC 4 cut(s) 20, 687, 1502, 2399
SspI AATATT 2 cut(s) 708, 1606
SspMI CTAG 4 cut(s) 545, 596, 747, 1013
StyD4I CCNGG 5 cut(s) 865, 1696, 1971, 2003, 2924
TaiI ACGT 1 cut(s) 2513
TaqI TCGA 8 cut(s) 108, 276, 716, 1672, 1998, 2238, 2632, 2736
TatI WGTACW 5 cut(s) 332, 592, 844, 1270, 1489
TauI GCSGC 1 cut(s) 690
TfiI GAWTC 7 cut(s) 827, 1036, 1796, 2146, 2425, 2687, 2849
Tru1I TTAA 8 cut(s) 416, 789, 1347, 1368, 1379, 1427, 1740, 2340
Tru9I TTAA 8 cut(s) 416, 789, 1347, 1368, 1379, 1427, 1740, 2340
TseI GCWGC 6 cut(s) 533, 674, 1406, 2108, 2566, 2909
TspDTI ATGAA 6 cut(s) 143, 1028, 1659, 1805, 2451, 2679
TspGWI ACGGA 2 cut(s) 663, 868
Van91I CCANNNNNTGG 1 cut(s) 1233
Vha464I CTTAAG 2 cut(s) 1346, 1367
VneI GTGCAC 1 cut(s) 2283
VpaK11BI GGWCC 2 cut(s) 2291, 2841
VspI ATTAAT 1 cut(s) 1740
XapI RAATTY 2 cut(s) 430, 451
XbaI TCTAGA 1 cut(s) 544
XceI RCATGY 5 cut(s) 254, 488, 1844, 1952, 2726
XcmI CCANNNNNNNNNTGG 2 cut(s) 13, 2214
XmiI GTMKAC 2 cut(s) 1983, 2328
XmnI GAANNNNTTC 1 cut(s) 874
XspI CTAG 4 cut(s) 545, 596, 747, 1013
ZrmI AGTACT 3 cut(s) 334, 846, 1491
Zsp2I ATGCAT 4 cut(s) 628, 1723, 1846, 2878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.