Rorug02G0516100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003119
Physical Location & Seq
Forward (+)
64755215 .. 64756269
1055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0516100.1

Sequence Viewer

Length: 594 bp
ATGAAGAAGCTCTACAACAAGAAAGGCAAGGTCCACCCGTCGCCACCGCCTTCGACACCCGACCCGTTCGCCCTGCTTCCTGCCACCATTTTGACTCTGACCACCGCCCTCTCTGTCCAGGACCGAGAGGTCCTCGCCTATCTCATCTCCTACACCAACAACAACAACAATTCGACCAGCATCAACACCAACAAGGCCAACGATGCCCATAAAGCTGGAAACGATCACCCTGATCCTCCGATGTCCCAGTGTGACTGCTTTAGGTGCTACAAGAGCTTCTGGGCACGATGGGATGCTTCACCCAACCGGCAGCTCATACATGAAATCATCGAGGCGTACGAGGAAAAGTTGGCTGAGAAGAAAAGGAGAAATCCCAAGGTGAAGAAAGTGAGGAAGAAGAAAATTGAGGAGGAAACGAAGGGTAATATTGATAATCTACCGGTCATGAAAGAGGAGCTGAGTAATAATCCAGAAGTGGATGTGGAAAACTGTGATGGTGATGAAGAAGGCTGTGAGGTTGAGGTTGAGAAAGGATCCTCCATGAGGAGGTTGGCGAGCTTCATTGGAGATAGGATTTGGGGTGTTTGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

22.43

Weight (kDa)

6.9

Isoelectric Point (pI)

51.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AciI CCGC 2 cut(s) 47, 105
AclWI GGATC 3 cut(s) 227, 528, 541
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 2 cut(s) 543, 546
AgeI ACCGGT 1 cut(s) 439
AjnI CCWGG 1 cut(s) 117
AluBI AGCT 6 cut(s) 10, 215, 276, 313, 457, 558
AluI AGCT 6 cut(s) 10, 215, 276, 313, 457, 558
AlwI GGATC 3 cut(s) 227, 528, 541
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 1 cut(s) 310
AsiGI ACCGGT 1 cut(s) 439
AspS9I GGNCC 3 cut(s) 31, 121, 130
AsuHPI GGTGA 4 cut(s) 218, 291, 391, 509
AvaII GGWCC 3 cut(s) 31, 121, 130
BaeGI GKGCMC 1 cut(s) 286
BamHI GGATCC 1 cut(s) 533
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbvI GCAGC 1 cut(s) 322
BccI CCATC 2 cut(s) 282, 488
BciT130I CCWGG 1 cut(s) 119
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 3 cut(s) 31, 121, 130
BmgT120I GGNCC 3 cut(s) 31, 121, 130
BmiI GGNNCC 1 cut(s) 535
BmrFI CCNGG 1 cut(s) 119
BmrI ACTGGG 1 cut(s) 241
BmsI GCATC 3 cut(s) 189, 193, 283
BmuI ACTGGG 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BsaJI CCNNGG 1 cut(s) 375
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 2 cut(s) 543, 546
Bse118I RCCGGY 2 cut(s) 306, 439
Bse1I ACTGG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 2 cut(s) 298, 484
BseLI CCNNNNNNNGG 2 cut(s) 543, 546
BseMII CTCAG 2 cut(s) 345, 449
BseNI ACTGG 1 cut(s) 247
BseRI GAGGAG 3 cut(s) 422, 467, 559
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 1 cut(s) 322
BshFI GGCC 1 cut(s) 197
BshTI ACCGGT 1 cut(s) 439
BsiSI CCGG 2 cut(s) 307, 440
BsiWI CGTACG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 2 cut(s) 543, 546
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 3 cut(s) 223, 232, 533
BspACI CCGC 2 cut(s) 47, 105
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 2 cut(s) 346, 450
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 535
BspPI GGATC 3 cut(s) 227, 528, 541
BsrFI RCCGGY 2 cut(s) 306, 439
BsrI ACTGG 1 cut(s) 247
BssAI RCCGGY 2 cut(s) 306, 439
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 3 cut(s) 223, 232, 533
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 1 cut(s) 491
BstC8I GCNNGC 1 cut(s) 556
BstDEI CTNAG 2 cut(s) 354, 458
BstENI CCTNNNNNAGG 1 cut(s) 541
BstF5I GGATG 2 cut(s) 298, 484
BstKTI GATC 3 cut(s) 226, 235, 536
BstMBI GATC 3 cut(s) 223, 232, 533
BstMWI GCNNNNNNNGC 4 cut(s) 203, 212, 264, 273
BstNI CCWGG 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 286
BstV1I GCAGC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 533
BstXI CCANNNNNNTGG 1 cut(s) 215
BstYI RGATCY 1 cut(s) 533
BsuRI GGCC 1 cut(s) 197
BtsCI GGATG 2 cut(s) 298, 484
BtsIMutI CAGTG 1 cut(s) 254
Cac8I GCNNGC 1 cut(s) 556
CciI TCATGA 1 cut(s) 444
Cfr10I RCCGGY 2 cut(s) 306, 439
Cfr13I GGNCC 3 cut(s) 31, 121, 130
Csp6I GTAC 1 cut(s) 337
CspAI ACCGGT 1 cut(s) 439
CviAII CATG 3 cut(s) 320, 445, 541
CviJI RGCY 9 cut(s) 10, 197, 215, 276, 313, 353, 457, 510, 558
CviKI_1 RGCY 9 cut(s) 10, 197, 215, 276, 313, 353, 457, 510, 558
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 2 cut(s) 354, 458
DpnI GATC 3 cut(s) 225, 234, 535
DpnII GATC 3 cut(s) 223, 232, 533
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
Eco130I CCWWGG 1 cut(s) 375
Eco47I GGWCC 3 cut(s) 31, 121, 130
EcoNI CCTNNNNNAGG 1 cut(s) 541
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 323, 448, 544
FaiI YATR 5 cut(s) 210, 317, 321, 446, 542
FaqI GGGAC 1 cut(s) 229
FatI CATG 3 cut(s) 319, 444, 540
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 2 cut(s) 305, 491
Fsp4HI GCNGC 1 cut(s) 311
GluI GCNGC 1 cut(s) 311
HaeIII GGCC 1 cut(s) 197
HapII CCGG 2 cut(s) 307, 440
Hin1II CATG 3 cut(s) 323, 448, 544
HinfI GANTC 1 cut(s) 94
HpaII CCGG 2 cut(s) 307, 440
HphI GGTGA 4 cut(s) 218, 291, 391, 509
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 2 cut(s) 99, 240
Hpy188III TCNNGA 2 cut(s) 445, 470
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 3 cut(s) 60, 412, 500
HpyCH4III ACNGT 1 cut(s) 491
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 212, 264, 273
HpyF3I CTNAG 2 cut(s) 354, 458
Hsp92II CATG 3 cut(s) 323, 448, 544
Kzo9I GATC 3 cut(s) 223, 232, 533
LmnI GCTCC 1 cut(s) 454
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 3 cut(s) 189, 193, 283
MaeIII GTNAC 1 cut(s) 251
MalI GATC 3 cut(s) 225, 234, 535
MboI GATC 3 cut(s) 223, 232, 533
MboII GAAGA 6 cut(s) 16, 370, 394, 406, 409, 515
MflI RGATCY 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 3 cut(s) 169, 402, 589
MlyI GAGTC 1 cut(s) 88
MspI CCGG 2 cut(s) 307, 440
MspR9I CCNGG 1 cut(s) 119
MvaI CCWGG 1 cut(s) 119
MwoI GCNNNNNNNGC 4 cut(s) 203, 212, 264, 273
NdeII GATC 3 cut(s) 223, 232, 533
NlaIII CATG 3 cut(s) 323, 448, 544
NlaIV GGNNCC 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 251
PagI TCATGA 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 336
Pfl23II CGTACG 1 cut(s) 336
PfoI TCCNGGA 1 cut(s) 117
PinAI ACCGGT 1 cut(s) 439
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PpuMI RGGWCCY 1 cut(s) 130
Psp5II RGGWCCY 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspLI CGTACG 1 cut(s) 336
PspN4I GGNNCC 1 cut(s) 535
PspPI GGNCC 3 cut(s) 31, 121, 130
PspPPI RGGWCCY 1 cut(s) 130
PsuI RGATCY 1 cut(s) 533
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 3 cut(s) 223, 232, 533
Sau96I GGNCC 3 cut(s) 31, 121, 130
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 3 cut(s) 189, 193, 283
SinI GGWCC 3 cut(s) 31, 121, 130
Sse9I AATT 3 cut(s) 169, 402, 589
SsiI CCGC 2 cut(s) 47, 105
SspI AATATT 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 1 cut(s) 491
TaqI TCGA 3 cut(s) 53, 173, 330
TaqII GACCGA 1 cut(s) 138
TasI AATT 3 cut(s) 169, 402, 589
TscAI CASTG 1 cut(s) 254
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 5 cut(s) 17, 336, 461, 516, 550
TspRI CASTG 1 cut(s) 254
VpaK11BI GGWCC 3 cut(s) 31, 121, 130
XagI CCTNNNNNAGG 1 cut(s) 541
XcmI CCANNNNNNNNNTGG 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.