Rroxscaffold_3G00270590

SPIRAL1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003119
Physical Location & Seq
Reverse (-)
63151570 .. 63154294
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00270590.1

Sequence Viewer

Length: 312 bp
ATGACAACTCACGGAAGGTGGCGGCACGGTGGGTTGAAGGGGAGGTCAGAATCCCGCCCCAAAAAGGTTGAAACTCCTAAGGTTCAGGTGGAGGTTGAACCTACTCAGAAGCCTACTGCTGCGGCACCACCTGCGGAAGTGATCAATAAGGAGACTCCTGCAGGCGTTCCTGGGAACACTTCAAACAACTACTTCCGAGCTGATGGCCAGAACCGTGGAAACTTCCTCACGGATCGTCCATCCACAAAGGTTCATTCAGCTCCTGGTGGGGGTTCATCTCTAGATTACCTGTTTGGAGGTGGTGGCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

10.93

Weight (kDa)

9.89

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 124
AciI CCGC 4 cut(s) 22, 55, 122, 134
AclWI GGATC 1 cut(s) 240
AcoI YGGCCR 2 cut(s) 205, 304
AfiI CCNNNNNNNGG 2 cut(s) 64, 269
AgsI TTSAA 4 cut(s) 37, 71, 98, 183
AjnI CCWGG 2 cut(s) 169, 262
AluBI AGCT 2 cut(s) 200, 260
AluI AGCT 2 cut(s) 200, 260
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 1 cut(s) 240
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 2 cut(s) 205, 304
ApeKI GCWGC 1 cut(s) 119
AxyI CCTNAGG 1 cut(s) 78
BalI TGGCCA 2 cut(s) 207, 306
BanI GGYRCC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 106
BccI CCATC 2 cut(s) 197, 247
BciT130I CCWGG 2 cut(s) 171, 264
BclI TGATCA 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 159
BfuAI ACCTGC 1 cut(s) 139
BisI GCNGC 3 cut(s) 23, 120, 123
BlsI GCNGC 3 cut(s) 24, 121, 124
Bme1390I CCNGG 2 cut(s) 171, 264
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 2 cut(s) 171, 264
BsaJI CCNNGG 2 cut(s) 170, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 269
Bse1I ACTGG 1 cut(s) 307
Bse21I CCTNAGG 1 cut(s) 78
BseBI CCWGG 2 cut(s) 171, 264
BseDI CCNNGG 2 cut(s) 170, 214
BseGI GGATG 1 cut(s) 239
BseLI CCNNNNNNNGG 2 cut(s) 64, 269
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 106
BshFI GGCC 2 cut(s) 207, 306
BshNI GGYRCC 1 cut(s) 124
BslI CCNNNNNNNGG 2 cut(s) 64, 269
BsmAI GTCTC 1 cut(s) 146
BsnI GGCC 2 cut(s) 207, 306
Bsp143I GATC 2 cut(s) 141, 232
BspACI CCGC 4 cut(s) 22, 55, 122, 134
BspANI GGCC 2 cut(s) 207, 306
BspCNI CTCAG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 124
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 2 cut(s) 170, 214
BssMI GATC 2 cut(s) 141, 232
Bst2UI CCWGG 2 cut(s) 171, 264
Bst4CI ACNGT 2 cut(s) 29, 215
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 2 cut(s) 78, 105
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 1 cut(s) 239
BstKTI GATC 2 cut(s) 144, 235
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 2 cut(s) 141, 232
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 2 cut(s) 171, 264
BstSCI CCNGG 2 cut(s) 169, 262
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 106
BstXI CCANNNNNNTGG 1 cut(s) 215
Bsu36I CCTNAGG 1 cut(s) 78
BsuRI GGCC 2 cut(s) 207, 306
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 239
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 163
CaiI CAGNNNCTG 1 cut(s) 263
CviJI RGCY 5 cut(s) 112, 200, 207, 260, 306
CviKI_1 RGCY 5 cut(s) 112, 200, 207, 260, 306
DdeI CTNAG 2 cut(s) 78, 105
DpnI GATC 2 cut(s) 143, 234
DpnII GATC 2 cut(s) 141, 232
EaeI YGGCCR 2 cut(s) 205, 304
Eco81I CCTNAGG 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 169, 262
FauI CCCGC 1 cut(s) 62
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 3 cut(s) 23, 120, 123
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 3 cut(s) 23, 120, 123
FspBI CTAG 1 cut(s) 281
GluI GCNGC 3 cut(s) 23, 120, 123
HaeIII GGCC 2 cut(s) 207, 306
HinfI GANTC 2 cut(s) 50, 154
Hpy188I TCNGA 3 cut(s) 49, 108, 197
Hpy188III TCNNGA 1 cut(s) 281
HpyAV CCTTC 2 cut(s) 9, 31
HpyCH4III ACNGT 2 cut(s) 29, 215
HpyCH4V TGCA 1 cut(s) 161
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 2 cut(s) 78, 105
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 141, 232
LmnI GCTCC 1 cut(s) 265
Lsp1109I GCAGC 1 cut(s) 106
MaeI CTAG 1 cut(s) 281
MalI GATC 2 cut(s) 143, 234
MboI GATC 2 cut(s) 141, 232
MlsI TGGCCA 2 cut(s) 207, 306
MluNI TGGCCA 2 cut(s) 207, 306
MlyI GAGTC 1 cut(s) 148
MnlI CCTC 4 cut(s) 36, 85, 236, 290
Mox20I TGGCCA 2 cut(s) 207, 306
MscI TGGCCA 2 cut(s) 207, 306
Msp20I TGGCCA 2 cut(s) 207, 306
MspR9I CCNGG 2 cut(s) 171, 264
MvaI CCWGG 2 cut(s) 171, 264
MwoI GCNNNNNNNGC 1 cut(s) 131
NdeII GATC 2 cut(s) 141, 232
NlaIV GGNNCC 1 cut(s) 126
PaqCI CACCTGC 1 cut(s) 139
PfeI GAWTC 1 cut(s) 50
PkrI GCNGC 3 cut(s) 24, 121, 124
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
Psp6I CCWGG 2 cut(s) 169, 262
PspGI CCWGG 2 cut(s) 169, 262
PspN4I GGNNCC 1 cut(s) 126
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 263
SatI GCNGC 3 cut(s) 23, 120, 123
Sau3AI GATC 2 cut(s) 141, 232
SbfI CCTGCAGG 1 cut(s) 163
SchI GAGTC 1 cut(s) 148
ScrFI CCNGG 2 cut(s) 171, 264
SdaI CCTGCAGG 1 cut(s) 163
SfcI CTRYAG 1 cut(s) 159
Sse8387I CCTGCAGG 1 cut(s) 163
SsiI CCGC 4 cut(s) 22, 55, 122, 134
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 2 cut(s) 169, 262
TaaI ACNGT 2 cut(s) 29, 215
TauI GCSGC 2 cut(s) 25, 125
TfiI GAWTC 1 cut(s) 50
TseI GCWGC 1 cut(s) 119
TspDTI ATGAA 2 cut(s) 242, 264
TspGWI ACGGA 2 cut(s) 27, 245
XbaI TCTAGA 1 cut(s) 280
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.