Rorug06G0456100

SPIRAL1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003119
Physical Location & Seq
Forward (+)
58382461 .. 58387275
4815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0456100.1

Sequence Viewer

Length: 1326 bp
ATGGCTTCTATTAGAAGAACATTGTCTCCAGTGCCTCGAGCAGGAATTCCACTAAATGGGGAAGTCCATTCAGTTGCTTCTCCATTGTCCAAGTCCTCATCTTGTACTCAGAACTATCCATCATCCAATGGATTCTTATCATCTTTGGCTGCTTCATTGGATTCTCAAGCGTTTGTTCTCGGTGTTTTTTCACCGAGGTCTTATAGGCCTTTTGAGAAATCAAAACCCAAGGGGCAAGTTTGGAGAAGGGCGCTTTCCCATTTCTTTATTTGTTTCATGCTTGGAGTCTTTATTGGGGCTACACCTTTTGCGTCAATGACTTTATCCACAAATCTCATGTCAAAGCATCAGGACTTATCCTTTGAAATGATATCCTCAGTCGGGTTTCATCAGTTGCATGGGAGTGTATCCAGAAATGTGACACCATTAGATAGCAGGGAAATGCAGAAGAATGTTACAATGGAGTCACAAGTCAAGGAGTGGGAACCAACAGAAGGCATCCTATTAAAAACTAATATGCAGAACCAATCACTCATTCAAGAAGCAGATATGGAGTTCCGAAAGCTTTTGGTAATCGTGACTCCAACATATGCTCATCCATTTCGGGCCTATAATCTGAATCGTTTGGCACACACGTTAAAATTAGTCTCCCCTCCATTGTTATGGATCGTTGTGGAGATGACCTCCCAATCAGCAGAAACAACTGACATCTTGAGGAGAACTGGGATTATGTACAGACATCTTGTGTGCGAGAAAAATCTTACTGATGTAAGAGACAGAAGTGTTCATCAAAGAAACGTTGCACTTTTTCACATTGAAAGTCACCGTCTTGATGGAATCATCTACTTTGCAGATGAGGACAACATCTACTCAAGTGATCTTTTTGAGCAAATGAGGCACATTAGGCGGTTTGGAACATGGTCAGTTGCCAAAATAACAGGTAACAAAATGCAAGCTGCTATAGAGGGTCCCGTTTGTAATGGAACTCAAGTAATTGGATGGCATGCAATTGAATCAAGTATAAGATTTCAAAGATTCTATGCTCAAATATCAGGATTTGCCTTCAATAGTACTATACTCTGGGATCCCAAAAGGTGGCATCGACCGACTCTTGAACCTATTAGGCAGCTTGACACAGGCAATGATGGTTTCCAGGTGAGCACATTTATTGAACAAGTTGTGGAAGATGAAAGTCAAATGGAAGGTTTGCTGCAAGATTGCTCGAGGATCATGGTTTGGCATCGTCATCATGAATCAGTTAATGCCTTCTATCCTCACAAGTGGTTCAAGAAGAATAATCTAGATGTCATTGCTTCACTTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

441

Amino Acids

50.37

Weight (kDa)

8.88

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_43 PF03360 209 - 416 9.7e-68 Glycosyltransferase family 43
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 56, 1095
AciI CCGC 1 cut(s) 907
AclI AACGTT 1 cut(s) 798
AclWI GGATC 4 cut(s) 674, 1079, 1092, 1235
AcsI RAATTY 1 cut(s) 45
AfaI GTAC 3 cut(s) 106, 734, 1072
AfiI CCNNNNNNNGG 5 cut(s) 41, 56, 381, 494, 1095
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 9 cut(s) 365, 539, 818, 1013, 1031, 1066, 1115, 1172, 1288
AjnI CCWGG 1 cut(s) 1152
AjuI GAANNNNNNNTTGG 2 cut(s) 519, 551
AleI CACNNNNGTG 1 cut(s) 1321
AloI GAACNNNNNNTCC 2 cut(s) 10, 42
AluBI AGCT 3 cut(s) 565, 956, 1129
AluI AGCT 3 cut(s) 565, 956, 1129
Alw21I GWGCWC 1 cut(s) 1163
Alw26I GTCTC 3 cut(s) 30, 652, 768
AlwI GGATC 4 cut(s) 674, 1079, 1092, 1235
Ama87I CYCGRG 2 cut(s) 36, 1222
AoxI GGCC 2 cut(s) 206, 606
ApeKI GCWGC 4 cut(s) 149, 956, 1126, 1210
ApoI RAATTY 1 cut(s) 45
ArsI GACNNNNNNTTYG 4 cut(s) 323, 344, 355, 376
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 2 cut(s) 606, 968
AsuHPI GGTGA 3 cut(s) 183, 815, 1168
AvaI CYCGRG 2 cut(s) 36, 1222
AvaII GGWCC 1 cut(s) 968
BamHI GGATCC 1 cut(s) 1084
Bbv12I GWGCWC 1 cut(s) 1163
BbvI GCAGC 4 cut(s) 136, 943, 1138, 1197
BccI CCATC 4 cut(s) 127, 827, 993, 1139
BciT130I CCWGG 1 cut(s) 1154
BciVI GTATCC 1 cut(s) 418
BcoDI GTCTC 3 cut(s) 30, 652, 768
BfaI CTAG 1 cut(s) 1301
BfmI CTRYAG 1 cut(s) 960
BfoI RGCGCY 1 cut(s) 254
BfuI GTATCC 1 cut(s) 418
BisI GCNGC 4 cut(s) 150, 957, 1127, 1211
BlsI GCNGC 4 cut(s) 151, 958, 1128, 1212
BmcAI AGTACT 1 cut(s) 1072
Bme1390I CCNGG 1 cut(s) 1154
Bme18I GGWCC 1 cut(s) 968
BmeT110I CYCGRG 2 cut(s) 36, 1222
BmgT120I GGNCC 2 cut(s) 606, 968
BmiI GGNNCC 4 cut(s) 486, 969, 970, 1086
BmrFI CCNGG 1 cut(s) 1154
BmrI ACTGGG 1 cut(s) 732
BmsI GCATC 4 cut(s) 355, 507, 1108, 1249
BmuI ACTGGG 1 cut(s) 732
BplI GAGNNNNNCTC 2 cut(s) 668, 700
BpmI CTGGAG 1 cut(s) 12
BpuEI CTTGAG 4 cut(s) 150, 733, 856, 972
BsaAI YACGTR 1 cut(s) 1323
BsaBI GATNNNNATC 1 cut(s) 136
BsaJI CCNNGG 2 cut(s) 194, 228
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 56, 381, 494, 1095
Bse1I ACTGG 2 cut(s) 29, 727
Bse3DI GCAATG 2 cut(s) 1147, 1308
Bse8I GATNNNNATC 1 cut(s) 136
BseBI CCWGG 1 cut(s) 1154
BseDI CCNNGG 2 cut(s) 194, 228
BseGI GGATG 4 cut(s) 122, 498, 595, 1004
BseJI GATNNNNATC 1 cut(s) 136
BseLI CCNNNNNNNGG 5 cut(s) 41, 56, 381, 494, 1095
BseMI GCAATG 2 cut(s) 1147, 1308
BseMII CTCAG 2 cut(s) 122, 390
BseNI ACTGG 2 cut(s) 29, 727
BseRI GAGGAG 1 cut(s) 730
BseXI GCAGC 4 cut(s) 136, 943, 1138, 1197
Bsh1285I CGRYCG 1 cut(s) 1106
BshFI GGCC 2 cut(s) 208, 608
BsiEI CGRYCG 1 cut(s) 1106
BsiHKAI GWGCWC 1 cut(s) 1163
BsiHKCI CYCGRG 2 cut(s) 36, 1222
BslFI GGGAC 1 cut(s) 954
BslI CCNNNNNNNGG 5 cut(s) 41, 56, 381, 494, 1095
BsmAI GTCTC 3 cut(s) 30, 652, 768
BsmFI GGGAC 1 cut(s) 954
BsnI GGCC 2 cut(s) 208, 608
BsoBI CYCGRG 2 cut(s) 36, 1222
Bsp1286I GDGCHC 1 cut(s) 1163
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 4 cut(s) 666, 877, 1084, 1227
BspACI CCGC 1 cut(s) 907
BspANI GGCC 2 cut(s) 208, 608
BspCNI CTCAG 2 cut(s) 121, 389
BspHI TCATGA 1 cut(s) 1249
BspLI GGNNCC 4 cut(s) 486, 969, 970, 1086
BspPI GGATC 4 cut(s) 674, 1079, 1092, 1235
BsrDI GCAATG 2 cut(s) 1147, 1308
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 2 cut(s) 29, 727
BssECI CCNNGG 2 cut(s) 194, 228
BssMI GATC 4 cut(s) 666, 877, 1084, 1227
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 1154
Bst4CI ACNGT 1 cut(s) 827
BstAUI TGTACA 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 1323
BstC8I GCNNGC 2 cut(s) 954, 1005
BstDEI CTNAG 2 cut(s) 108, 376
BstENI CCTNNNNNAGG 1 cut(s) 39
BstF5I GGATG 4 cut(s) 122, 498, 595, 1004
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 4 cut(s) 669, 880, 1087, 1230
BstMAI GTCTC 3 cut(s) 30, 652, 768
BstMBI GATC 4 cut(s) 666, 877, 1084, 1227
BstMCI CGRYCG 1 cut(s) 1106
BstMWI GCNNNNNNNGC 2 cut(s) 895, 904
BstNI CCWGG 1 cut(s) 1154
BstNSI RCATGY 1 cut(s) 1007
BstSCI CCNGG 1 cut(s) 1152
BstSFI CTRYAG 1 cut(s) 960
BstV1I GCAGC 4 cut(s) 136, 943, 1138, 1197
BstX2I RGATCY 1 cut(s) 1084
BstXI CCANNNNNNTGG 1 cut(s) 663
BstYI RGATCY 1 cut(s) 1084
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 2 cut(s) 208, 608
BtsCI GGATG 4 cut(s) 122, 498, 595, 1004
BtsIMutI CAGTG 1 cut(s) 36
Cac8I GCNNGC 2 cut(s) 954, 1005
CciI TCATGA 1 cut(s) 1249
CfoI GCGC 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 606, 968
CseI GACGC 1 cut(s) 300
Csp6I GTAC 3 cut(s) 105, 733, 1071
CviAII CATG 7 cut(s) 277, 337, 398, 918, 1004, 1231, 1250
CviJI RGCY 8 cut(s) 5, 149, 208, 299, 565, 608, 956, 1129
CviKI_1 RGCY 8 cut(s) 5, 149, 208, 299, 565, 608, 956, 1129
CviQI GTAC 3 cut(s) 105, 733, 1071
DdeI CTNAG 2 cut(s) 108, 376
DpnI GATC 4 cut(s) 668, 879, 1086, 1229
DpnII GATC 4 cut(s) 666, 877, 1084, 1227
Eco130I CCWWGG 1 cut(s) 228
Eco147I AGGCCT 1 cut(s) 208
Eco32I GATATC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 968
Eco88I CYCGRG 2 cut(s) 36, 1222
EcoNI CCTNNNNNAGG 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 968
EcoRI GAATTC 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 1152
EcoRV GATATC 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 7 cut(s) 280, 340, 401, 921, 1007, 1234, 1253
FaqI GGGAC 1 cut(s) 954
FatI CATG 7 cut(s) 276, 336, 397, 917, 1003, 1230, 1249
FauNDI CATATG 1 cut(s) 589
Fnu4HI GCNGC 4 cut(s) 150, 957, 1127, 1211
FokI GGATG 4 cut(s) 109, 485, 582, 1011
Fsp4HI GCNGC 4 cut(s) 150, 957, 1127, 1211
FspBI CTAG 1 cut(s) 1301
GlaI GCGC 1 cut(s) 252
GluI GCNGC 4 cut(s) 150, 957, 1127, 1211
GsuI CTGGAG 1 cut(s) 12
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 2 cut(s) 208, 608
HgaI GACGC 1 cut(s) 300
HhaI GCGC 1 cut(s) 253
Hin1II CATG 7 cut(s) 280, 340, 401, 921, 1007, 1234, 1253
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HindIII AAGCTT 1 cut(s) 563
HphI GGTGA 3 cut(s) 183, 815, 1168
Hpy188I TCNGA 3 cut(s) 111, 560, 618
HpyAV CCTTC 5 cut(s) 240, 488, 1072, 1196, 1276
HpyCH4III ACNGT 1 cut(s) 827
HpyCH4IV ACGT 3 cut(s) 635, 798, 1322
HpyCH4V TGCA 8 cut(s) 397, 445, 520, 803, 851, 952, 1007, 1213
HpyF10VI GCNNNNNNNGC 2 cut(s) 895, 904
HpyF3I CTNAG 2 cut(s) 108, 376
HpySE526I ACGT 3 cut(s) 635, 798, 1322
Hsp92II CATG 7 cut(s) 280, 340, 401, 921, 1007, 1234, 1253
HspAI GCGC 1 cut(s) 251
KflI GGGWCCC 1 cut(s) 968
Kzo9I GATC 4 cut(s) 666, 877, 1084, 1227
Lsp1109I GCAGC 4 cut(s) 136, 943, 1138, 1197
LweI GCATC 4 cut(s) 355, 507, 1108, 1249
MaeI CTAG 1 cut(s) 1301
MaeII ACGT 3 cut(s) 635, 798, 1322
MaeIII GTNAC 6 cut(s) 418, 454, 465, 577, 821, 941
MalI GATC 4 cut(s) 668, 879, 1086, 1229
MboI GATC 4 cut(s) 666, 877, 1084, 1227
MboII GAAGA 4 cut(s) 27, 460, 1196, 1303
MfeI CAATTG 1 cut(s) 1008
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 1 cut(s) 1163
MluCI AATT 4 cut(s) 45, 641, 993, 1008
MlyI GAGTC 4 cut(s) 294, 473, 574, 1102
MmeI TCCRAC 1 cut(s) 608
MseI TTAA 3 cut(s) 506, 638, 1260
MslI CAYNNNNRTG 3 cut(s) 402, 661, 1321
MspR9I CCNGG 1 cut(s) 1154
MunI CAATTG 1 cut(s) 1008
MvaI CCWGG 1 cut(s) 1154
MwoI GCNNNNNNNGC 2 cut(s) 895, 904
NdeI CATATG 1 cut(s) 589
NdeII GATC 4 cut(s) 666, 877, 1084, 1227
NlaIII CATG 7 cut(s) 280, 340, 401, 921, 1007, 1234, 1253
NlaIV GGNNCC 4 cut(s) 486, 969, 970, 1086
NmuCI GTSAC 4 cut(s) 418, 465, 577, 821
NspI RCATGY 1 cut(s) 1007
OliI CACNNNNGTG 1 cut(s) 1321
PaeI GCATGC 1 cut(s) 1007
PaeR7I CTCGAG 2 cut(s) 36, 1222
PagI TCATGA 1 cut(s) 1249
PceI AGGCCT 1 cut(s) 208
PfeI GAWTC 7 cut(s) 132, 161, 619, 837, 1013, 1035, 1253
PflMI CCANNNNNTGG 2 cut(s) 56, 1095
PkrI GCNGC 4 cut(s) 151, 958, 1128, 1212
PleI GAGTC 4 cut(s) 293, 472, 574, 1102
PpsI GAGTC 4 cut(s) 293, 472, 574, 1102
Ppu21I YACGTR 1 cut(s) 1323
PpuMI RGGWCCY 1 cut(s) 968
Psp1406I AACGTT 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 968
Psp6I CCWGG 1 cut(s) 1152
PspGI CCWGG 1 cut(s) 1152
PspN4I GGNNCC 4 cut(s) 486, 969, 970, 1086
PspPI GGNCC 2 cut(s) 606, 968
PspPPI RGGWCCY 1 cut(s) 968
PspXI VCTCGAGB 2 cut(s) 36, 1222
PsuI RGATCY 1 cut(s) 1084
RsaI GTAC 3 cut(s) 106, 734, 1072
RsaNI GTAC 3 cut(s) 105, 733, 1071
RseI CAYNNNNRTG 3 cut(s) 402, 661, 1321
SaqAI TTAA 3 cut(s) 506, 638, 1260
SatI GCNGC 4 cut(s) 150, 957, 1127, 1211
Sau3AI GATC 4 cut(s) 666, 877, 1084, 1227
Sau96I GGNCC 2 cut(s) 606, 968
ScaI AGTACT 1 cut(s) 1072
SchI GAGTC 4 cut(s) 294, 473, 574, 1102
ScrFI CCNGG 1 cut(s) 1154
SduI GDGCHC 1 cut(s) 1163
SfaNI GCATC 4 cut(s) 355, 507, 1108, 1249
SfcI CTRYAG 1 cut(s) 960
Sfr274I CTCGAG 2 cut(s) 36, 1222
SinI GGWCC 1 cut(s) 968
SlaI CTCGAG 2 cut(s) 36, 1222
SmiMI CAYNNNNRTG 3 cut(s) 402, 661, 1321
SmlI CTYRAG 6 cut(s) 36, 165, 712, 871, 987, 1222
SmoI CTYRAG 6 cut(s) 36, 165, 712, 871, 987, 1222
SphI GCATGC 1 cut(s) 1007
Sse9I AATT 4 cut(s) 45, 641, 993, 1008
SseBI AGGCCT 1 cut(s) 208
SsiI CCGC 1 cut(s) 907
SspMI CTAG 1 cut(s) 1301
StuI AGGCCT 1 cut(s) 208
StyD4I CCNGG 1 cut(s) 1152
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 827
TaiI ACGT 3 cut(s) 638, 801, 1325
TaqI TCGA 3 cut(s) 37, 1102, 1223
TaqII GACCGA 1 cut(s) 1120
TasI AATT 4 cut(s) 45, 641, 993, 1008
TatI WGTACW 3 cut(s) 104, 732, 1070
TfiI GAWTC 7 cut(s) 132, 161, 619, 837, 1013, 1035, 1253
Tru1I TTAA 3 cut(s) 506, 638, 1260
Tru9I TTAA 3 cut(s) 506, 638, 1260
TscAI CASTG 1 cut(s) 36
TseFI GTSAC 4 cut(s) 418, 465, 577, 821
TseI GCWGC 4 cut(s) 149, 956, 1126, 1210
Tsp45I GTSAC 4 cut(s) 418, 465, 577, 821
TspDTI ATGAA 6 cut(s) 144, 265, 377, 776, 1203, 1266
TspRI CASTG 1 cut(s) 36
Van91I CCANNNNNTGG 2 cut(s) 56, 1095
VpaK11BI GGWCC 1 cut(s) 968
XagI CCTNNNNNAGG 1 cut(s) 39
XapI RAATTY 1 cut(s) 45
XbaI TCTAGA 1 cut(s) 1300
XceI RCATGY 1 cut(s) 1007
XhoI CTCGAG 2 cut(s) 36, 1222
XspI CTAG 1 cut(s) 1301
ZrmI AGTACT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.