MD14G1213800.v1.1

SPIRAL1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003119
Physical Location & Seq
Forward (+)
29816645 .. 29818388
1744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1213800.v1.1.491

Sequence Viewer

Length: 366 bp
ATGGGTCGTGGAGTCAGCAGCGGAGGGGGAGAAAGCTCTTTGGGCTATCTGTTTGGAGGTGGAGAAGAAACTCAGAATCCCGTGGCGAAAAGGGGTGCAGCTCCTCAGAACCAGGCGCCGGTTCCAACTGCCGAACCACCTGCTCAGAGGGCTACTGCTCCCGCACCACCTGCAGAAGTCAATAAGGAGATTCCCGCTGGCGTACCTGGGAACACTTCAAACAACTATTTCCGAGCTGATGGCCAGAACTGCGGCAACTTCATCACAGATCGACCATCAACGAAAGTGCACTCAGCTCCCGGTGGTGGATCATCCCTAGACTACCTGTTTGGAGGCGGTGGCGGTGGCGGTGGCGGTGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

11.83

Weight (kDa)

6.55

Isoelectric Point (pI)

58.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 148, 178
Acc36I ACCTGC 2 cut(s) 148, 178
AccB1I GGYRCC 1 cut(s) 115
AciI CCGC 8 cut(s) 21, 162, 195, 252, 336, 342, 348, 354
AclWI GGATC 1 cut(s) 316
AcoI YGGCCR 1 cut(s) 241
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 1 cut(s) 204
AfiI CCNNNNNNNGG 2 cut(s) 118, 305
AgsI TTSAA 1 cut(s) 219
AjnI CCWGG 2 cut(s) 111, 205
AluBI AGCT 4 cut(s) 36, 101, 236, 296
AluI AGCT 4 cut(s) 36, 101, 236, 296
Alw21I GWGCWC 1 cut(s) 291
Alw44I GTGCAC 1 cut(s) 287
AlwI GGATC 1 cut(s) 316
AoxI GGCC 1 cut(s) 241
ApaLI GTGCAC 1 cut(s) 287
ApeKI GCWGC 2 cut(s) 18, 98
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AspLEI GCGC 1 cut(s) 118
AsuC2I CCSGG 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 291
BalI TGGCCA 1 cut(s) 243
BanI GGYRCC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 2 cut(s) 30, 110
BccI CCATC 2 cut(s) 233, 283
BcgI CGANNNNNNTGC 2 cut(s) 122, 156
BciT130I CCWGG 2 cut(s) 113, 207
BcnI CCSGG 1 cut(s) 300
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 119
BfuAI ACCTGC 2 cut(s) 148, 178
BisI GCNGC 3 cut(s) 19, 99, 253
BlsI GCNGC 3 cut(s) 20, 100, 254
Bme1390I CCNGG 3 cut(s) 113, 207, 300
BmiI GGNNCC 2 cut(s) 117, 123
BmrFI CCNGG 3 cut(s) 113, 207, 300
BpuMI CCSGG 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 81, 206
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 305
Bse118I RCCGGY 1 cut(s) 118
BseBI CCWGG 2 cut(s) 113, 207
BseDI CCNNGG 2 cut(s) 81, 206
BseGI GGATG 1 cut(s) 311
BseLI CCNNNNNNNGG 2 cut(s) 118, 305
BseMII CTCAG 4 cut(s) 86, 119, 158, 306
BseRI GAGGAG 1 cut(s) 93
BseSI GKGCMC 1 cut(s) 291
BseXI GCAGC 2 cut(s) 30, 110
BsgI GTGCAG 1 cut(s) 117
BshFI GGCC 1 cut(s) 243
BshNI GGYRCC 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 291
BsiSI CCGG 2 cut(s) 119, 300
BslI CCNNNNNNNGG 2 cut(s) 118, 305
BsnI GGCC 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 2 cut(s) 268, 308
BspACI CCGC 8 cut(s) 21, 162, 195, 252, 336, 342, 348, 354
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 4 cut(s) 85, 118, 157, 305
BspLI GGNNCC 2 cut(s) 117, 123
BspMAI CTGCAG 1 cut(s) 175
BspMI ACCTGC 2 cut(s) 148, 178
BspPI GGATC 1 cut(s) 316
BspT107I GGYRCC 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 81, 206
BssMI GATC 2 cut(s) 268, 308
BssNI GRCGYC 1 cut(s) 116
Bst2UI CCWGG 2 cut(s) 113, 207
BstACI GRCGYC 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 170
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 4 cut(s) 72, 105, 144, 292
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 2 cut(s) 271, 311
BstMBI GATC 2 cut(s) 268, 308
BstMWI GCNNNNNNNGC 4 cut(s) 42, 149, 170, 249
BstNI CCWGG 2 cut(s) 113, 207
BstSCI CCNGG 3 cut(s) 111, 205, 298
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 30, 110
BsuRI GGCC 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 311
BveI ACCTGC 2 cut(s) 148, 178
Cac8I GCNNGC 1 cut(s) 199
CfoI GCGC 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 118
Csp6I GTAC 1 cut(s) 203
CviJI RGCY 7 cut(s) 36, 45, 101, 152, 236, 243, 296
CviKI_1 RGCY 7 cut(s) 36, 45, 101, 152, 236, 243, 296
CviQI GTAC 1 cut(s) 203
DdeI CTNAG 4 cut(s) 72, 105, 144, 292
DinI GGCGCC 1 cut(s) 117
DpnI GATC 2 cut(s) 270, 310
DpnII GATC 2 cut(s) 268, 308
EaeI YGGCCR 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 111, 205
EgeI GGCGCC 1 cut(s) 117
EheI GGCGCC 1 cut(s) 117
FauI CCCGC 2 cut(s) 169, 202
Fnu4HI GCNGC 3 cut(s) 19, 99, 253
FokI GGATG 1 cut(s) 298
Fsp4HI GCNGC 3 cut(s) 19, 99, 253
FspBI CTAG 1 cut(s) 317
GlaI GCGC 1 cut(s) 117
GluI GCNGC 3 cut(s) 19, 99, 253
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 1 cut(s) 243
HapII CCGG 2 cut(s) 119, 300
HhaI GCGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 116
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HinfI GANTC 3 cut(s) 12, 76, 190
HpaII CCGG 2 cut(s) 119, 300
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 4 cut(s) 75, 108, 147, 233
Hpy8I GTNNAC 1 cut(s) 289
HpyCH4V TGCA 3 cut(s) 98, 173, 289
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 149, 170, 249
HpyF3I CTNAG 4 cut(s) 72, 105, 144, 292
Hsp92I GRCGYC 1 cut(s) 116
HspAI GCGC 1 cut(s) 116
KasI GGCGCC 1 cut(s) 115
Kzo9I GATC 2 cut(s) 268, 308
LmnI GCTCC 3 cut(s) 106, 163, 301
Lsp1109I GCAGC 2 cut(s) 30, 110
MaeI CTAG 1 cut(s) 317
MalI GATC 2 cut(s) 270, 310
MboI GATC 2 cut(s) 268, 308
MboII GAAGA 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 291
MlsI TGGCCA 1 cut(s) 243
MluNI TGGCCA 1 cut(s) 243
Mly113I GGCGCC 1 cut(s) 116
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 5 cut(s) 17, 50, 114, 141, 326
Mox20I TGGCCA 1 cut(s) 243
MscI TGGCCA 1 cut(s) 243
Msp20I TGGCCA 1 cut(s) 243
MspA1I CMGCKG 2 cut(s) 21, 197
MspI CCGG 2 cut(s) 119, 300
MspR9I CCNGG 3 cut(s) 113, 207, 300
MvaI CCWGG 2 cut(s) 113, 207
MwoI GCNNNNNNNGC 4 cut(s) 42, 149, 170, 249
NarI GGCGCC 1 cut(s) 116
NciI CCSGG 1 cut(s) 300
NdeII GATC 2 cut(s) 268, 308
NlaIV GGNNCC 2 cut(s) 117, 123
PaqCI CACCTGC 2 cut(s) 148, 178
PfeI GAWTC 2 cut(s) 76, 190
PkrI GCNGC 3 cut(s) 20, 100, 254
PleI GAGTC 1 cut(s) 20
PluTI GGCGCC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 111, 205
PspGI CCWGG 2 cut(s) 111, 205
PspN4I GGNNCC 2 cut(s) 117, 123
PstI CTGCAG 1 cut(s) 175
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
SatI GCNGC 3 cut(s) 19, 99, 253
Sau3AI GATC 2 cut(s) 268, 308
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 3 cut(s) 113, 207, 300
SduI GDGCHC 1 cut(s) 291
SetI ASST 9 cut(s) 38, 61, 103, 142, 172, 208, 238, 298, 327
SfcI CTRYAG 1 cut(s) 171
SfoI GGCGCC 1 cut(s) 117
SsiI CCGC 8 cut(s) 21, 162, 195, 252, 336, 342, 348, 354
SspDI GGCGCC 1 cut(s) 115
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 3 cut(s) 111, 205, 298
TaqI TCGA 1 cut(s) 271
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 2 cut(s) 76, 190
TseI GCWGC 2 cut(s) 18, 98
TspDTI ATGAA 1 cut(s) 250
VneI GTGCAC 1 cut(s) 287
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.