FvH4_5g14341

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006789
Physical Location & Seq
Reverse (-)
8111063 .. 8121255
10193 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14341.t3

Sequence Viewer

Length: 3756 bp
ATGGAAATCACCGAAGAACAGCGAAAGCGAGCCGAAGCGAATCGATTAGCAGCCTTAGCCAAACGAAAAGCCTTGGCCGAATCCTCCTCCGCCTCCGCCCTCCACCAACAACCCGACCCGTGGTCGCTTTTCAAGTGCCGGAAGCTCTCTCCGGACCTCAACCCGAGCTCAATTCGCTGCGCCGGCGAACCAAGATCCGAAATTTCACGGGCCAACCCGGTTGTGGAGAACCAGATGTTCCGGGTCCGGCTCGAAATCTGCTCACCGGACTCATTCTCAGCCACCCCGGTCGGAATTCAAGGGTTCGCATTTCCGGGGCAGGAAGAGTGCTCGAGGAGATTGAGTGATTGCTTGGCTAATGTGATGCCATCACACTACACGCAGAATCACGGTGGTGGAAAGGCCGGTGTTTATAAGCTGAGTGAGTACAAACAAGTCGTGAGATGTTTGAGGAGCAACAAGGGCATTGACATTGAAGAGATACCTTGGACTACTTTCAATGTTGTGGAGAGGCTTTCACAGTCGTATATCACAGGGAAGTGGGTACCATGTAGGCCAGAACACGTGTCAGATGATAAGGTTGATGAATTGATTGGGAAGCTGCCAAAGAAGCTATTTGATTCACTCTTGCCTTTCCAGCTTGAGGGTGTTAGATTTGGTTTGCAAAGGGGTGGACGATGTCTCATTGCAGACGAAATGGGCCTTGGAAAGACACTACAGGCTATTGCTATTGCATGCTGCTTCATGAGTGAAGGCTCTATACTCGTTGTTTGCCCAGCTATTTTGCGTTATTCATGGGCAGAAGAATTAGAGCGTTGGCTTCCTTTTTGTTTGCCTGCTGACATCCATCTAGTTTTTGGTCACGAAAACAATCCTGCCAATTTAAAGAAATGGCCTAGAATTGTGGTTATTTCTTATACAATGCTTCACCATTTGCAAAAGAGCATGCTAGATCGAGAATGGGCTCTCTTGATTGTTGATGAATCCCACCATGTACGATGTACGAAAAAAAAATCAGAACCTAAAGAGATACAAGCTGTTCTTGATGTGGCAAGAAAGGTCGAGCGCGTAGTTTTACTATCGGGGACACCTTCTTTGTCAAGGCCATTTGACATTTTTCATCAGATAGATATGCTATGGCCTGGTTTGCTGGGAAAGGACAAGTTTAAGTTTGCTGAAACTTACTGTGATGCCAAATTTGTTCGGGGTGTCCAAGGGAAAATTTTTCAGGATTTCTCAAAGGGTACTCGTTTGGAGGAGTTGAACATGTTACTCAAGCAAACAGTTATGATTAGACGTTTGAAGGAACATGTCCTATCTCAGCTACCACCCAAACGTCGACAAATTATACAAGTTGTACTGAAGAAATCAGATATAGTTTCAGCAAAGGCTGCTATCAGGGTGGGAAAGAGTTGCAATGAAGATGTATCTTCGGAGCAGTTAGATGAACCAAATGATAATATGGGTTGCTGCATATCAAAGCAGCTTTCTTACCAAGAATTAGGTATTGCAAAGCTAGCCGGGTTTCGTGAATGGCTCTCTATTCATCCAGTCATTGCAGAGTCAAACGGGGTGGCAAACTTGGAGTCGGATTCCAGTTCTCATAAAATGTTAATATTTGCACATCACCATAAAGTTCTTGATGGGGTACAGGAGTTCATACTCCAGAAAGAGATAGATTTTGTCCGGATTGACGGAAACACACTTGCTACAGATAGGCAGTTAGCTGTGCGAAAATTCCAACTATCAACTGAGGTTAAGATTGCAGTAGTTGGTATTACCGCGGGAGGTGTTGGTCTTGATTTCTCATCAGCAACACACGTTGTCTTCTTGGAGCTTCCTCAGTCCCCATCTTTGATGCTTCAGGCTGAGGATAGAGCACACAGGCGAGGGCAAACAAATGCAGTCAACATATACTTCTTTACTGGGAAGGATACCATAGATGAGTCACATTGGCAGTACTTGAACAGGAGTTTGAGCCGTGTTTCATCTACAACAAATGGAAAATATGATGCAATACAAGAAATAGCGGTTGAAGATGTTTCTTTTTTTGAGACATTACGTGGAGTTGGTAATTGTGAAGATTTTATTTTGCAAAAGTCAGAGGGTAGTGAGTTCTCTGCAGAACTAATGAAAGTGCCTGGTTCTGACTGCTTAGCTAAGGCTATGAAGCCTTCTGAGTCAAATGATAAGTTAGTGCCAAACATTCCTCAGAGATCTGAAAGACATCATGGTACTGATGGCATTTCATCACAAACTGAAACTTCTGTTAAGAACGATGTGGTCTCTGATTGGGATATGGACAACAGTATCTCTTCAGATGAGGAGTTGGAAACAATTGTTCCTGAGACTAAAATTAGAGAATGGAGAGTGTCTTCTTGTAAGTTTAGTAAGAGCGGTGAAGGTAGAGTTGGAAAGGAATCACTAGATAAGAAGCAGGAAGATGGAATCAACTCTCAGACCAGCAAAGGACATGATGACGGATCTGTTTTGCCAATCAAAGGACATGTAGACGAACCTGTTCAGCAAATTGAAGCTGGAGAAGGTGGCATTAACCAAGTAGATGCTCTTCGATTTGAGGTGAGTCAATACACTGGTAGGATCCACTTGTATTCTTGCATCTCAGGAGAGGATGCAAGACCAAGGCCACTTTTTGAAAATTTTAGACCAGAGGAACTTCAGTCACTATATTCTTCTGCTGCTGAAAGTATTAAAGGAACAGCTTCAAATTCGATCAAGGATAACCCTGCTTATTTGCATGCACTTCTTGCATTCAATGAAGAATGGAAGAAACTGAGGCCCATTGAACAAAAGAAATTAATTGGAAAGCCTTTGCAACTTCCTTTGTCTGTTGAGTTGTGCTTTCTGTGTGAAGGCACTAACCATGAGAAAACGGGACTGCTGAAGGGCCGAAGCAAGCGCCGGTCTACACCTTATGATGAGATTAGCAAACCCTTACCATCAAATGCTGTGTGGAAAAAGGTCCACCTGCGTAGTGGCTATGGAAAAAAGGAGAAGCAATACACTCAGGGTTACACTTTGACTGATGAGCCTCTCTGTAAACTCTGCCAAACACCATGCGAGGGCCACAATGCCAAGGAACCTGAATATATTGAAGACCTGTTCTGTAATCTTGACTGTTATGGAGAGTACCACATCCGAACTAGTAACAGATCTATTCGTCACGAACTTTTTCAACTAGAACGCGGTGTTTGTGTAAATTGTCAATTGGACTGTCATAAACTTGTGGAGCACATACGACCTTTATCTGATGAAAAGCGGAGGCAGTATATTGAAAAGTTTGCACCAAGAGTGGCAAGGCTGAAGAAGTTGCTTGAGAGGCTTGTAAAGGATCCAACTGAGGGAAATGCATGGCATGCAGATCACCTTGTTCCTGTCTATCTTGGTGGAGGTGAATGCAGGCTAGAGAACATGAGGACTCTGTGTGTGGCCTGTCATTCTGATGTTACTAGAGCACAATGTGCTGAACGGCGGTCAACAAGGAGCAAGGCTAAGAAACAACTGAAAGAAATCATGAGTGAAATGAAAAACAAAGACACAGAGATCAATCTAAAGGACCAGGGGGATTCAGAGACAGAGAAGAATTCAAGTGATAACGAACTTTTAGTCAATGTTCCTGGGAGTGCATACTCTTTAGCAAATTATGTGGATGCTACAACGATGAATGAAGAGCTGGAAGACGCCTCCAATAGCGAGAAGACTCCCACAGTAGAAAAGCACCTGGGTGGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

1252

Amino Acids

140.84

Weight (kDa)

6.64

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 213 - 458 2.6e-29 SNF2-related domain
ResIII PF04851 223 - 364 2e-07 Type III restriction enzyme, res subunit
DEAD PF00270 223 - 367 3.6e-07 DEAD/DEAH box helicase
Helicase_C PF00271 530 - 631 2.2e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AarI CACCTGC 1 cut(s) 2997
Acc36I ACCTGC 1 cut(s) 2997
Acc65I GGTACC 1 cut(s) 544
AccB1I GGYRCC 1 cut(s) 544
AccBSI CCGCTC 1 cut(s) 2397
AccI GTMKAC 3 cut(s) 1339, 2511, 2927
AccII CGCG 3 cut(s) 1068, 1784, 3207
AccIII TCCGGA 2 cut(s) 151, 1686
AciI CCGC 9 cut(s) 90, 96, 1782, 1784, 2030, 2397, 3207, 3280, 3493
AclWI GGATC 6 cut(s) 189, 2491, 2596, 2609, 3347, 3360
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 8 cut(s) 201, 294, 1196, 1221, 1736, 2659, 2728, 3604
AcuI CTGAAG 6 cut(s) 1382, 1847, 2301, 2663, 2924, 3344
AcvI CACGTG 1 cut(s) 565
AcyI GRCGYC 1 cut(s) 3702
AdeI CACNNNGTG 1 cut(s) 3482
AfiI CCNNNNNNNGG 6 cut(s) 120, 223, 643, 2501, 3083, 3362
AflIII ACRYGT 6 cut(s) 562, 564, 1266, 1309, 1819, 2506
AhdI GACNNNNNGTC 1 cut(s) 121
AhlI ACTAGT 1 cut(s) 3164
AjnI CCWGG 5 cut(s) 1141, 2140, 3579, 3637, 3741
AjuI GAANNNNNNNTTGG 4 cut(s) 687, 719, 2394, 2426
AleI CACNNNNGTG 2 cut(s) 393, 3744
Alw21I GWGCWC 5 cut(s) 170, 332, 1882, 3255, 3478
Alw26I GTCTC 5 cut(s) 686, 2048, 2290, 2342, 3587
AlwI GGATC 6 cut(s) 189, 2491, 2596, 2609, 3347, 3360
Ama87I CYCGRG 2 cut(s) 163, 331
Aor13HI TCCGGA 2 cut(s) 151, 1686
ApeKI GCWGC 8 cut(s) 50, 177, 601, 738, 1391, 1470, 1483, 2699
ApoI RAATTY 8 cut(s) 201, 294, 1196, 1221, 1736, 2659, 2728, 3604
ArsI GACNNNNNNTTYG 6 cut(s) 1078, 1110, 1571, 1603, 3612, 3644
AseI ATTAAT 1 cut(s) 2819
Asp700I GAANNNNTTC 3 cut(s) 2520, 2722, 3192
Asp718I GGTACC 1 cut(s) 544
AspLEI GCGC 3 cut(s) 182, 1068, 2922
AspS9I GGNCC 9 cut(s) 154, 210, 244, 700, 2799, 2908, 2983, 3085, 3577
AsuC2I CCSGG 5 cut(s) 218, 242, 287, 315, 1522
AsuHPI GGTGA 7 cut(s) 255, 920, 1619, 2411, 2593, 3377, 3425
AsuNHI GCTAGC 1 cut(s) 1516
AvaI CYCGRG 2 cut(s) 163, 331
AvaII GGWCC 4 cut(s) 154, 244, 2983, 3577
BaeI ACNNNNGTAYC 4 cut(s) 1640, 1673, 2293, 2326
BamHI GGATCC 2 cut(s) 2601, 3352
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 2 cut(s) 170, 967
BarI GAAGNNNNNNTAC 4 cut(s) 744, 776, 2553, 2585
BbrPI CACGTG 1 cut(s) 565
BbsI GAAGAC 5 cut(s) 1819, 2367, 3123, 3705, 3725
Bbv12I GWGCWC 5 cut(s) 170, 332, 1882, 3255, 3478
BbvCI CCTCAGC 1 cut(s) 1869
BbvI GCAGC 8 cut(s) 62, 164, 588, 725, 1378, 1457, 1495, 2686
BccI CCATC 7 cut(s) 376, 855, 1637, 1858, 2234, 2438, 2968
BceAI ACGGC 2 cut(s) 1965, 3506
BciT130I CCWGG 5 cut(s) 1143, 2142, 3581, 3639, 3743
BciVI GTATCC 1 cut(s) 1927
BcnI CCSGG 5 cut(s) 218, 242, 287, 315, 1522
BcoDI GTCTC 5 cut(s) 686, 2048, 2290, 2342, 3587
BcuI ACTAGT 1 cut(s) 3164
BfaI CTAG 9 cut(s) 851, 897, 950, 1517, 2426, 3165, 3200, 3425, 3471
BfmI CTRYAG 3 cut(s) 716, 1710, 2121
BfoI RGCGCY 1 cut(s) 2923
BfuAI ACCTGC 1 cut(s) 2997
BfuI GTATCC 1 cut(s) 1927
BglII AGATCT 2 cut(s) 2216, 3173
BisI GCNGC 8 cut(s) 51, 178, 602, 739, 1392, 1471, 1484, 2700
BlpI GCTNAGC 1 cut(s) 2155
BlsI GCNGC 8 cut(s) 52, 179, 603, 740, 1393, 1472, 1485, 2701
BmcAI AGTACT 1 cut(s) 1961
Bme18I GGWCC 4 cut(s) 154, 244, 2983, 3577
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 2 cut(s) 163, 331
BmgT120I GGNCC 9 cut(s) 154, 210, 244, 700, 2799, 2908, 2983, 3085, 3577
BmiI GGNNCC 5 cut(s) 245, 546, 2603, 3102, 3354
BmrI ACTGGG 1 cut(s) 1935
BmsI GCATC 8 cut(s) 354, 1180, 1848, 2002, 2554, 2623, 2628, 3661
BmtI GCTAGC 1 cut(s) 1520
BmuI ACTGGG 1 cut(s) 1935
BpiI GAAGAC 5 cut(s) 1819, 2367, 3123, 3705, 3725
BpmI CTGGAG 2 cut(s) 1649, 2559
Bpu10I CCTNAGC 3 cut(s) 55, 1869, 2160
Bpu1102I GCTNAGC 1 cut(s) 2155
BpuEI CTTGAG 3 cut(s) 662, 1259, 3356
BpuMI CCSGG 5 cut(s) 218, 242, 287, 315, 1522
Bsa29I ATCGAT 1 cut(s) 43
BsaAI YACGTR 2 cut(s) 565, 2063
BsaHI GRCGYC 1 cut(s) 3702
BsaI GGTCTC 1 cut(s) 2290
BsaWI WCCGGW 3 cut(s) 151, 265, 1686
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 6 cut(s) 120, 223, 643, 2501, 3083, 3362
Bse118I RCCGGY 3 cut(s) 182, 404, 2922
Bse1I ACTGG 4 cut(s) 1550, 1596, 1930, 2599
Bse3DI GCAATG 3 cut(s) 684, 1423, 1554
BseAI TCCGGA 2 cut(s) 151, 1686
BseBI CCWGG 5 cut(s) 1143, 2142, 3581, 3639, 3743
BseCI ATCGAT 1 cut(s) 43
BseGI GGATG 5 cut(s) 843, 1546, 2638, 3156, 3676
BseLI CCNNNNNNNGG 6 cut(s) 120, 223, 643, 2501, 3083, 3362
BseMI GCAATG 3 cut(s) 684, 1423, 1554
BseNI ACTGG 4 cut(s) 1550, 1596, 1930, 2599
BseRI GAGGAG 5 cut(s) 76, 349, 466, 1271, 2339
BseXI GCAGC 8 cut(s) 62, 164, 588, 725, 1378, 1457, 1495, 2686
BseYI CCCAGC 2 cut(s) 775, 1150
Bsh1236I CGCG 3 cut(s) 1068, 1784, 3207
Bsh1285I CGRYCG 1 cut(s) 291
BshNI GGYRCC 1 cut(s) 544
BshVI ATCGAT 1 cut(s) 43
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 5 cut(s) 170, 332, 1882, 3255, 3478
BsiHKCI CYCGRG 2 cut(s) 163, 331
BslFI GGGAC 3 cut(s) 1099, 1831, 2910
BslI CCNNNNNNNGG 6 cut(s) 120, 223, 643, 2501, 3083, 3362
BsmAI GTCTC 5 cut(s) 686, 2048, 2290, 2342, 3587
BsmFI GGGAC 3 cut(s) 1099, 1831, 2910
BsmI GAATGC 2 cut(s) 2771, 3422
Bso31I GGTCTC 1 cut(s) 2290
BsoBI CYCGRG 2 cut(s) 163, 331
Bsp1286I GDGCHC 6 cut(s) 170, 332, 967, 1882, 3255, 3478
Bsp13I TCCGGA 2 cut(s) 151, 1686
Bsp1720I GCTNAGC 1 cut(s) 2155
BspACI CCGC 9 cut(s) 90, 96, 1782, 1784, 2030, 2397, 3207, 3280, 3493
BspDI ATCGAT 1 cut(s) 43
BspEI TCCGGA 2 cut(s) 151, 1686
BspFNI CGCG 3 cut(s) 1068, 1784, 3207
BspHI TCATGA 2 cut(s) 744, 3534
BspLI GGNNCC 5 cut(s) 245, 546, 2603, 3102, 3354
BspMAI CTGCAG 1 cut(s) 2125
BspMI ACCTGC 1 cut(s) 2997
BspOI GCTAGC 1 cut(s) 1520
BspPI GGATC 6 cut(s) 189, 2491, 2596, 2609, 3347, 3360
BspQI GCTCTTC 2 cut(s) 2574, 3684
BspT107I GGYRCC 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 2290
BsrBI CCGCTC 1 cut(s) 2397
BsrDI GCAATG 3 cut(s) 684, 1423, 1554
BsrFI RCCGGY 3 cut(s) 182, 404, 2922
BsrI ACTGG 4 cut(s) 1550, 1596, 1930, 2599
BssAI RCCGGY 3 cut(s) 182, 404, 2922
BssNI GRCGYC 1 cut(s) 3702
BssT1I CCWWGG 6 cut(s) 72, 485, 703, 1213, 2642, 3096
Bst2UI CCWGG 5 cut(s) 1143, 2142, 3581, 3639, 3743
Bst4CI ACNGT 8 cut(s) 392, 522, 1187, 1285, 2309, 3140, 3236, 3730
Bst6I CTCTTC 5 cut(s) 318, 471, 2320, 2574, 3684
BstACI GRCGYC 1 cut(s) 3702
BstAPI GCANNNNNTGC 4 cut(s) 1391, 2768, 3377, 3482
BstBAI YACGTR 2 cut(s) 565, 2063
BstDSI CCRYGG 2 cut(s) 119, 1782
BstF5I GGATG 5 cut(s) 843, 1546, 2638, 3156, 3676
BstFNI CGCG 3 cut(s) 1068, 1784, 3207
BstH2I RGCGCY 1 cut(s) 2923
BstHHI GCGC 3 cut(s) 182, 1068, 2922
BstMAI GTCTC 5 cut(s) 686, 2048, 2290, 2342, 3587
BstMCI CGRYCG 1 cut(s) 291
BstNI CCWGG 5 cut(s) 1143, 2142, 3581, 3639, 3743
BstNSI RCATGY 7 cut(s) 738, 949, 1270, 1313, 2510, 2762, 3380
BstSFI CTRYAG 3 cut(s) 716, 1710, 2121
BstUI CGCG 3 cut(s) 1068, 1784, 3207
BstV1I GCAGC 8 cut(s) 62, 164, 588, 725, 1378, 1457, 1495, 2686
BstV2I GAAGAC 5 cut(s) 1819, 2367, 3123, 3705, 3725
BstX2I RGATCY 6 cut(s) 194, 2216, 2483, 2601, 3173, 3352
BstYI RGATCY 6 cut(s) 194, 2216, 2483, 2601, 3173, 3352
Bsu15I ATCGAT 1 cut(s) 43
BsuI GTATCC 1 cut(s) 1927
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 2 cut(s) 119, 1782
BtsCI GGATG 5 cut(s) 843, 1546, 2638, 3156, 3676
BtsIMutI CAGTG 1 cut(s) 2592
BveI ACCTGC 1 cut(s) 2997
CciI TCATGA 2 cut(s) 744, 3534
CfoI GCGC 3 cut(s) 182, 1068, 2922
Cfr10I RCCGGY 3 cut(s) 182, 404, 2922
Cfr13I GGNCC 9 cut(s) 154, 210, 244, 700, 2799, 2908, 2983, 3085, 3577
Cfr42I CCGCGG 1 cut(s) 1785
ClaI ATCGAT 1 cut(s) 43
CseI GACGC 1 cut(s) 3710
CspCI CAANNNNNGTGG 4 cut(s) 1554, 1589, 3648, 3683
DraI TTTAAA 1 cut(s) 885
DraIII CACNNNGTG 1 cut(s) 3482
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 5 cut(s) 318, 471, 2320, 2574, 3684
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 5 cut(s) 318, 471, 2320, 2574, 3684
EciI GGCGGA 2 cut(s) 79, 85
Ecl136II GAGCTC 1 cut(s) 168
Eco130I CCWWGG 6 cut(s) 72, 485, 703, 1213, 2642, 3096
Eco24I GRGCYC 2 cut(s) 170, 967
Eco31I GGTCTC 1 cut(s) 2290
Eco47I GGWCC 4 cut(s) 154, 244, 2983, 3577
Eco53kI GAGCTC 1 cut(s) 168
Eco57I CTGAAG 6 cut(s) 1382, 1847, 2301, 2663, 2924, 3344
Eco72I CACGTG 1 cut(s) 565
Eco88I CYCGRG 2 cut(s) 163, 331
EcoICRI GAGCTC 1 cut(s) 168
EcoRI GAATTC 2 cut(s) 294, 3604
EcoRII CCWGG 5 cut(s) 1141, 2140, 3579, 3637, 3741
EcoT14I CCWWGG 6 cut(s) 72, 485, 703, 1213, 2642, 3096
EcoT22I ATGCAT 1 cut(s) 3373
EcoT38I GRGCYC 2 cut(s) 170, 967
ErhI CCWWGG 6 cut(s) 72, 485, 703, 1213, 2642, 3096
FalI AAGNNNNNCTT 2 cut(s) 1026, 1058
FaqI GGGAC 3 cut(s) 1099, 1831, 2910
FauI CCCGC 1 cut(s) 1777
FblI GTMKAC 3 cut(s) 1339, 2511, 2927
Fnu4HI GCNGC 8 cut(s) 51, 178, 602, 739, 1392, 1471, 1484, 2700
FokI GGATG 5 cut(s) 830, 1533, 2645, 3143, 3683
FriOI GRGCYC 2 cut(s) 170, 967
Fsp4HI GCNGC 8 cut(s) 51, 178, 602, 739, 1392, 1471, 1484, 2700
FspBI CTAG 9 cut(s) 851, 897, 950, 1517, 2426, 3165, 3200, 3425, 3471
GlaI GCGC 3 cut(s) 181, 1067, 2921
GluI GCNGC 8 cut(s) 51, 178, 602, 739, 1392, 1471, 1484, 2700
GsaI CCCAGC 2 cut(s) 779, 1154
GsuI CTGGAG 2 cut(s) 1649, 2559
HaeII RGCGCY 1 cut(s) 2923
HgaI GACGC 1 cut(s) 3710
HhaI GCGC 3 cut(s) 182, 1068, 2922
Hin1I GRCGYC 1 cut(s) 3702
Hin6I GCGC 3 cut(s) 180, 1066, 2920
HinP1I GCGC 3 cut(s) 180, 1066, 2920
HincII GTYRAC 3 cut(s) 1340, 1909, 3498
HindII GTYRAC 3 cut(s) 1340, 1909, 3498
HphI GGTGA 7 cut(s) 255, 920, 1619, 2411, 2593, 3377, 3425
Hpy166II GTNNAC 8 cut(s) 674, 1340, 1909, 2512, 2928, 2986, 3062, 3498
Hpy8I GTNNAC 8 cut(s) 674, 1340, 1909, 2512, 2928, 2986, 3062, 3498
Hpy99I CGWCG 1 cut(s) 1341
HpyAV CCTTC 9 cut(s) 746, 1101, 1297, 1924, 2184, 2396, 2537, 2867, 2899
HpyCH4III ACNGT 8 cut(s) 392, 522, 1187, 1285, 2309, 3140, 3236, 3730
HpyCH4IV ACGT 5 cut(s) 564, 1297, 1336, 1821, 2062
HpySE526I ACGT 5 cut(s) 564, 1297, 1336, 1821, 2062
Hsp92I GRCGYC 1 cut(s) 3702
HspAI GCGC 3 cut(s) 180, 1066, 2920
Kpn2I TCCGGA 2 cut(s) 151, 1686
KpnI GGTACC 1 cut(s) 548
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
KspI CCGCGG 1 cut(s) 1785
LguI GCTCTTC 2 cut(s) 2574, 3684
LmnI GCTCC 5 cut(s) 453, 1435, 1834, 3250, 3504
Lsp1109I GCAGC 8 cut(s) 62, 164, 588, 725, 1378, 1457, 1495, 2686
LweI GCATC 8 cut(s) 354, 1180, 1848, 2002, 2554, 2623, 2628, 3661
MaeI CTAG 9 cut(s) 851, 897, 950, 1517, 2426, 3165, 3200, 3425, 3471
MaeII ACGT 5 cut(s) 564, 1297, 1336, 1821, 2062
MaeIII GTNAC 8 cut(s) 860, 1269, 1947, 2682, 3032, 3167, 3182, 3466
MbiI CCGCTC 1 cut(s) 2397
MfeI CAATTG 2 cut(s) 2337, 3227
MflI RGATCY 6 cut(s) 194, 2216, 2483, 2601, 3173, 3352
MhlI GDGCHC 6 cut(s) 170, 332, 967, 1882, 3255, 3478
MlyI GAGTC 8 cut(s) 263, 1571, 1595, 1955, 2189, 2593, 3433, 3715
MmeI TCCRAC 6 cut(s) 271, 1569, 1765, 2310, 2392, 3380
Mph1103I ATGCAT 1 cut(s) 3373
MreI CGCCGGCG 1 cut(s) 182
MroI TCCGGA 2 cut(s) 151, 1686
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 3 cut(s) 2520, 2722, 3192
MseI TTAA 8 cut(s) 884, 1167, 1613, 1758, 2271, 2553, 2712, 2819
MslI CAYNNNNRTG 3 cut(s) 393, 3462, 3744
MspA1I CMGCKG 1 cut(s) 1784
MunI CAATTG 2 cut(s) 2337, 3227
Mva1269I GAATGC 2 cut(s) 2771, 3422
MvaI CCWGG 5 cut(s) 1143, 2142, 3581, 3639, 3743
MvnI CGCG 3 cut(s) 1068, 1784, 3207
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 5 cut(s) 218, 242, 287, 315, 1522
NgoMIV GCCGGC 1 cut(s) 182
NheI GCTAGC 1 cut(s) 1516
NlaIV GGNNCC 5 cut(s) 245, 546, 2603, 3102, 3354
NmuCI GTSAC 4 cut(s) 860, 1947, 2682, 3182
NsiI ATGCAT 1 cut(s) 3373
NspI RCATGY 7 cut(s) 738, 949, 1270, 1313, 2510, 2762, 3380
OliI CACNNNNGTG 2 cut(s) 393, 3744
PaeI GCATGC 4 cut(s) 738, 949, 2762, 3380
PaeR7I CTCGAG 1 cut(s) 331
PagI TCATGA 2 cut(s) 744, 3534
PaqCI CACCTGC 1 cut(s) 2997
PciI ACATGT 3 cut(s) 1266, 1309, 2506
PciSI GCTCTTC 2 cut(s) 2574, 3684
PctI GAATGC 2 cut(s) 2771, 3422
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 3 cut(s) 2520, 2722, 3192
PfeI GAWTC 9 cut(s) 40, 80, 385, 620, 983, 1592, 2420, 2448, 3587
PflFI GACNNNGTC 1 cut(s) 678
PkrI GCNGC 8 cut(s) 52, 179, 603, 740, 1393, 1472, 1485, 2701
PleI GAGTC 8 cut(s) 263, 1570, 1594, 1954, 2188, 2592, 3433, 3715
PmaCI CACGTG 1 cut(s) 565
PmlI CACGTG 1 cut(s) 565
PpsI GAGTC 8 cut(s) 263, 1570, 1594, 1954, 2188, 2592, 3433, 3715
Ppu21I YACGTR 2 cut(s) 565, 2063
PscI ACATGT 3 cut(s) 1266, 1309, 2506
PshBI ATTAAT 1 cut(s) 2819
PsiI TTATAA 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 170
Psp6I CCWGG 5 cut(s) 1141, 2140, 3579, 3637, 3741
PspCI CACGTG 1 cut(s) 565
PspFI CCCAGC 2 cut(s) 775, 1150
PspGI CCWGG 5 cut(s) 1141, 2140, 3579, 3637, 3741
PspN4I GGNNCC 5 cut(s) 245, 546, 2603, 3102, 3354
PspPI GGNCC 9 cut(s) 154, 210, 244, 700, 2799, 2908, 2983, 3085, 3577
PspXI VCTCGAGB 1 cut(s) 331
PstI CTGCAG 1 cut(s) 2125
PsuI RGATCY 6 cut(s) 194, 2216, 2483, 2601, 3173, 3352
PsyI GACNNNGTC 1 cut(s) 678
RseI CAYNNNNRTG 3 cut(s) 393, 3462, 3744
SacI GAGCTC 1 cut(s) 170
SacII CCGCGG 1 cut(s) 1785
SalI GTCGAC 1 cut(s) 1338
SapI GCTCTTC 2 cut(s) 2574, 3684
SaqAI TTAA 8 cut(s) 884, 1167, 1613, 1758, 2271, 2553, 2712, 2819
SatI GCNGC 8 cut(s) 51, 178, 602, 739, 1392, 1471, 1484, 2700
Sau96I GGNCC 9 cut(s) 154, 210, 244, 700, 2799, 2908, 2983, 3085, 3577
ScaI AGTACT 1 cut(s) 1961
SchI GAGTC 8 cut(s) 263, 1571, 1595, 1955, 2189, 2593, 3433, 3715
SduI GDGCHC 6 cut(s) 170, 332, 967, 1882, 3255, 3478
SfaNI GCATC 8 cut(s) 354, 1180, 1848, 2002, 2554, 2623, 2628, 3661
SfcI CTRYAG 3 cut(s) 716, 1710, 2121
Sfr274I CTCGAG 1 cut(s) 331
Sfr303I CCGCGG 1 cut(s) 1785
SgrAI CRCCGGYG 1 cut(s) 182
SgrBI CCGCGG 1 cut(s) 1785
SinI GGWCC 4 cut(s) 154, 244, 2983, 3577
SlaI CTCGAG 1 cut(s) 331
SmiMI CAYNNNNRTG 3 cut(s) 393, 3462, 3744
SmlI CTYRAG 4 cut(s) 331, 641, 1274, 3335
SmoI CTYRAG 4 cut(s) 331, 641, 1274, 3335
SpeI ACTAGT 1 cut(s) 3164
SphI GCATGC 4 cut(s) 738, 949, 2762, 3380
SsiI CCGC 9 cut(s) 90, 96, 1782, 1784, 2030, 2397, 3207, 3280, 3493
SspI AATATT 1 cut(s) 1617
SspMI CTAG 9 cut(s) 851, 897, 950, 1517, 2426, 3165, 3200, 3425, 3471
SstI GAGCTC 1 cut(s) 170
StyI CCWWGG 6 cut(s) 72, 485, 703, 1213, 2642, 3096
TaaI ACNGT 8 cut(s) 392, 522, 1187, 1285, 2309, 3140, 3236, 3730
TaiI ACGT 5 cut(s) 567, 1300, 1339, 1824, 2065
TaqI TCGA 8 cut(s) 43, 252, 332, 955, 1062, 1339, 2572, 2732
TatI WGTACW 3 cut(s) 426, 1357, 1959
TfiI GAWTC 9 cut(s) 40, 80, 385, 620, 983, 1592, 2420, 2448, 3587
Tru1I TTAA 8 cut(s) 884, 1167, 1613, 1758, 2271, 2553, 2712, 2819
Tru9I TTAA 8 cut(s) 884, 1167, 1613, 1758, 2271, 2553, 2712, 2819
TscAI CASTG 1 cut(s) 2599
TseFI GTSAC 4 cut(s) 860, 1947, 2682, 3182
TseI GCWGC 8 cut(s) 50, 177, 601, 738, 1391, 1470, 1483, 2699
Tsp45I GTSAC 4 cut(s) 860, 1947, 2682, 3182
TspGWI ACGGA 2 cut(s) 1710, 2496
TspRI CASTG 1 cut(s) 2599
Tth111I GACNNNGTC 1 cut(s) 678
VpaK11BI GGWCC 4 cut(s) 154, 244, 2983, 3577
VspI ATTAAT 1 cut(s) 2819
XapI RAATTY 8 cut(s) 201, 294, 1196, 1221, 1736, 2659, 2728, 3604
XceI RCATGY 7 cut(s) 738, 949, 1270, 1313, 2510, 2762, 3380
XcmI CCANNNNNNNNNTGG 3 cut(s) 220, 854, 2993
XhoI CTCGAG 1 cut(s) 331
XmiI GTMKAC 3 cut(s) 1339, 2511, 2927
XmnI GAANNNNTTC 3 cut(s) 2520, 2722, 3192
XspI CTAG 9 cut(s) 851, 897, 950, 1517, 2426, 3165, 3200, 3425, 3471
ZrmI AGTACT 1 cut(s) 1961
Zsp2I ATGCAT 1 cut(s) 3373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.