Rroxscaffold_3G00271270

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006789
Physical Location & Seq
Reverse (-)
63651722 .. 63663132
11411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271270.1

Sequence Viewer

Length: 3357 bp
ATGGAAATCACCGAAGAACAACGAAAGCGAGCAGAGGCGAATCGATTGGCAGCCTTAGCAAAACGCAAAGCCCTCGCCGAATCCTCCAGTCTTCAACAACAACACGATCCGTGGTCGCTTTTCAAATGCCGAAAGGTCTCTCCGGACCTCAATCCGAGCTCAATTCGCTGCGCCCGCGATCCAAGATTTGAAATTTCGCGGGTTAACCCGGTTGTGGAGAACCAGATGTTCCGGGTCAGGCTCGAAATCTGCTCGCCCGACTCCTTCTCAGCCACCCCGGTTGCCGTTCAAGGGTTTAAATTTCCGGGGCGGGAAGAGTGCGTAAGGAGAATGAGTGATTGCTTGGCTGGTGTGATGCCATCCCACTACACACAAAATCACAGTGGGGGAAAGGCTGGTGTTTATAAGCTGAGTGAGTACAAAGAAGTCTTGAGATGTTTAAGGAGCAACAAGGGCATTGACATTGAAGAGATACCTTGGACTACTTTCCATGTTGTAGACAGACTTTCACAGTCATATATCACAGGGAAGTGGGTACCATGCAGACCAGAACACTTGTCAGATGATAAGGTTGATGAGTTGATTGGGAAACTACCAAAGAGGCTATTGGATTCACTCTTGCCTTTCCAGCTTGAGGGTGTTAGATTTGGTTTGCAGAGGGGTGGACGATGCCTTATTGCAGACGAAATGGGTCTTGGAAAGACACTACAGATAAAAGCTGTTCTTGATGTGGCAAGAAAAGTCAAGCGTATAGTTTTACTATCGGGGACACCTTCTTTGTCAAGGCCTGGTTTGCTGGGAAGGGACAAGTTTAAATTTGGAGAAACTTACTGTGATGCCAAATATGTTCGGGGTGTCCAAGGGAAAGTTTTTCAGATAAGACGTTTGAAGGAACATGTCCTCTCTCAGCTACCACCCAAACGCCGACAGATTATACAAGTTGTACTAAAGAAATCAGATATAGTTTCAGCAAAGGCTGCTATCAGGGTGGGAAAGAGTTGCAATGAAGATGTATCTTCAGAACATTTGGATGAACCAAATGATAGTATGGGTTGCTGCATATCAAAGCAGCTTTCTCACCAAGAATTAGGTATTGCAAAGCTAGCCGGGTTTCGTGAATGGCTCTCCATTCATCCAGTCATTTCAGAGGCAGATAGGGTTGCAAACTTGGAGTCGGATTCCAGTTCTCATAAAATGTTAATATTTGCACATCACCATAAAGTACTTGACGGAGTACAGGAGTTCATAATCCACAAAGAGATAGATTTTGTCCGAATTGATGGAAACACACTTGCCACAGATAGGCAGTTAGCTGTGCGAAAATTCCAACTATCAACTGAGGTTAAGATTGCAATAGTTGGTATTACAGCGGGAGGTGTTGGTCTTGATTTCTCATCAGCAACACATGTTGTCTTCTTGGAGCTTCCTCAGTCACCATCATTGATGCTTCAGGCTGAGGATAGAGCTCACAGGCGAGGGCAAACAAATGCAGTCAACATATACTTCTTTTCTGGGAAGGATACCATTGATGAGTCACATTGGCAATACTTGAACAGGAGTTTGCGCCGTGTTTCATCTACAACAAATGGAAAATATGATGCAATACAAGAAATAGCGGTTGAAGATGTTTCTTTTTTTGAGACATTGGGTGGAGGTGATACATGTGAAGATTATACTTTGCAAAAGTCAGAGGGTAGTGAGTTCTCCGTAGAGCTAATCAAATTTCCTGGTTCTGACTGCTTAGCTAAGGCTATGAAGCCTTTTGAGTCAAATGATAAGTTAGTGCCAAACATTCCACAGAGATCTGAAAGACATCATGGTACTGATGGCATTTCTTCACAAACTGAAAATTCTGTTATTAAGGATGATGTGGTCTTTGATTTGGATATGGATAACAGTATCTCTTTAGATGAGAAGTTGGAAACAAATGTTCCTGAGACTAAAATTAGAGAAGGGAGAGTCTATTCTTGTAAGTTAAATAAGAGCGGTGAAGATAGAGATGGACTGGAATCACTGGATAAGGTACATTTGAAGCAGGAAGACGGAATCAAATCTCAGACAAACAAAGGACATGATGGCCAATTTGTTCAGCCGATCAAAGGACATGATGGCGAACCTGTTCAGCCAATTGAAGCTGAAGATGGTGGCACTAACCAAGTAGATGCTCTTCGATTTGAGGTGAGTCAATATACGGGTAGAATCCACTTGTATTCTTGCATCTCAGGAGAGGATTCAAGACCAAGGCCACTTTTTGAGAATTTTAGACCAGAGGAACTTGAGTCACTAAGTTCTTCTGCCGCTGAAAGTATTAAAGGAACAGCTTCAAATTCATTCAAGGATAACCCAGCTTATTTGCATGCACTTCTTGAATTCTATAAAGAATGGAAGAAACTGAGGCCCATTGAACAAAAGAAATTAATTGGAAAGCCTTTGCAACTTCCTTTGACTGTTGAGTTGTGCTTTCTGTGTGAAGGCACTAACCACGACAATACGGGACTGCTGAAGGGCCGAAGCAAGCGCCGCTCTACACCTTTTGATGAGATTAGCAAACCCTTACCATCAAATGCTGTGTGGAAAAAGGTCCACCTACGTAGTGGCTATGGAAAAAAGGAGAAGCAATACACTCAGGGTTACACTTTGACTGATGAACCTCTCTGTAAACTCTGCCAAACACCATGCGAGAGCCCCAATGCCAAGGAACCTGAATATTTTGAAGACCTGTTCTGTAATCTTGACTGTTATGGAGAGTACCGTATCCGAACTAGTAACAGATCTATTCGTCATGAACTTTTTCAACTAGAACGCGGTGTCTGTGCAAATTGTCAGTTGGACTGTCATAAACTTGTGGAGCACATAAGACCTTTATCTGATGTAAACCGGAGGCAGTATATTGAAAAGTTTGCACCAAGAGTGGCAAGGTTGAAGAAGTTGCGTGAGAGGCTTGTTAAGGATCCAACAGAGGGAAATGCATGGCATGCAGATCACCTTGTTCCTGTCTATCTTGGTGGAGGTGAATGCAGGTTAGAGAACATGAGGACTCTGTGTGTGGCCTGTCATTCTGATGTTACTAGAGCACAATGTGCTGAACGGCGGTCAACAAGGAGCAAGGCTAAGAAACAACTGAAAGCAATCATGAGTGACCTTAAAAACAAAGGCACAGAGATTAACCCAAAGGACCAGGGGGATCCAGAGACACAGGAGAATTTGAGTGATGACGAACTTTTAGTCAAGGTTCCTGGGAGTGCCTACTCATTAGCAAATCATGTGGATGCTACAACGACGAATGAAGACTTGGAAGAGCCCTCCAATAGTGAGAAGATTCCCACAGTAGAAAAATTCACACATACGCCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

1118

Amino Acids

126.02

Weight (kDa)

7.51

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 392 - 493 2.5e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
Acc36I ACCTGC 1 cut(s) 3009
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 535
AccBSI CCGCTC 2 cut(s) 1986, 2523
AccI GTMKAC 1 cut(s) 498
AccII CGCG 3 cut(s) 177, 199, 2805
AccIII TCCGGA 1 cut(s) 142
AclWI GGATC 6 cut(s) 101, 173, 2945, 2958, 3179, 3192
AcoI YGGCCR 1 cut(s) 2077
AcuI CTGAAG 4 cut(s) 1002, 1433, 2157, 2522
AdeI CACNNNGTG 1 cut(s) 3080
AfaI GTAC 8 cut(s) 419, 537, 945, 1224, 1236, 1822, 2025, 2750
AfiI CCNNNNNNNGG 6 cut(s) 214, 291, 634, 1302, 2099, 2960
AflIII ACRYGT 3 cut(s) 895, 1405, 1661
AhlI ACTAGT 1 cut(s) 2762
AjnI CCWGG 4 cut(s) 787, 1726, 3177, 3235
AjuI GAANNNNNNNTTGG 4 cut(s) 678, 710, 3275, 3307
Alw21I GWGCWC 4 cut(s) 161, 1468, 2853, 3076
Alw26I GTCTC 4 cut(s) 142, 1634, 1931, 3185
AlwI GGATC 6 cut(s) 101, 173, 2945, 2958, 3179, 3192
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 6 cut(s) 785, 2077, 2243, 2396, 2506, 3048
ApeKI GCWGC 5 cut(s) 50, 168, 977, 1056, 1069
ArsI GACNNNNNNTTYG 6 cut(s) 760, 792, 1157, 1189, 3210, 3242
AseI ATTAAT 1 cut(s) 2417
Asp700I GAANNNNTTC 3 cut(s) 2118, 2320, 2790
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 3 cut(s) 173, 1566, 2520
AspS9I GGNCC 5 cut(s) 145, 2397, 2506, 2581, 3175
AsuC2I CCSGG 5 cut(s) 209, 233, 278, 306, 1108
AsuHPI GGTGA 8 cut(s) 1070, 1205, 1425, 1667, 2000, 2191, 2975, 3023
AsuNHI GCTAGC 1 cut(s) 1102
AvaII GGWCC 3 cut(s) 145, 2581, 3175
BalI TGGCCA 1 cut(s) 2079
BamHI GGATCC 2 cut(s) 2950, 3184
BanI GGYRCC 1 cut(s) 535
BanII GRGCYC 4 cut(s) 161, 1468, 2687, 3303
BarI GAAGNNNNNNTAC 2 cut(s) 2151, 2183
BbsI GAAGAC 5 cut(s) 83, 1405, 2046, 2721, 3294
Bbv12I GWGCWC 4 cut(s) 161, 1468, 2853, 3076
BbvCI CCTCAGC 1 cut(s) 1455
BbvI GCAGC 5 cut(s) 62, 155, 964, 1043, 1081
BccI CCATC 9 cut(s) 367, 1274, 1444, 1820, 1994, 2069, 2102, 2135, 2566
BceAI ACGGC 3 cut(s) 269, 1551, 3104
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 4 cut(s) 789, 1728, 3179, 3237
BciVI GTATCC 2 cut(s) 1513, 2765
BcnI CCSGG 5 cut(s) 209, 233, 278, 306, 1108
BcoDI GTCTC 4 cut(s) 142, 1634, 1931, 3185
BcuI ACTAGT 1 cut(s) 2762
BfaI CTAG 5 cut(s) 1103, 2763, 2798, 3069, 3355
BfmI CTRYAG 1 cut(s) 707
BfoI RGCGCY 1 cut(s) 2521
BfuAI ACCTGC 1 cut(s) 3009
BfuI GTATCC 2 cut(s) 1513, 2765
BglII AGATCT 2 cut(s) 1802, 2771
BisI GCNGC 7 cut(s) 51, 169, 978, 1057, 1070, 2298, 2521
BlpI GCTNAGC 1 cut(s) 1741
BlsI GCNGC 7 cut(s) 52, 170, 979, 1058, 1071, 2299, 2522
BmcAI AGTACT 1 cut(s) 1224
Bme1390I CCNGG 9 cut(s) 209, 233, 278, 306, 789, 1108, 1728, 3179, 3237
Bme18I GGWCC 3 cut(s) 145, 2581, 3175
BmgT120I GGNCC 5 cut(s) 145, 2397, 2506, 2581, 3175
BmiI GGNNCC 5 cut(s) 537, 2700, 2952, 3186, 3234
BmrFI CCNGG 9 cut(s) 209, 233, 278, 306, 789, 1108, 1728, 3179, 3237
BmsI GCATC 8 cut(s) 345, 659, 826, 1434, 1588, 2152, 2226, 3259
BmtI GCTAGC 1 cut(s) 1106
BpiI GAAGAC 5 cut(s) 83, 1405, 2046, 2721, 3294
BpmI CTGGAG 1 cut(s) 70
Bpu10I CCTNAGC 3 cut(s) 55, 1455, 1746
Bpu1102I GCTNAGC 1 cut(s) 1741
BpuEI CTTGAG 3 cut(s) 451, 653, 2297
BpuMI CCSGG 5 cut(s) 209, 233, 278, 306, 1108
Bsa29I ATCGAT 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 2591
BsaI GGTCTC 1 cut(s) 142
BsaJI CCNNGG 9 cut(s) 110, 276, 305, 476, 859, 2240, 2694, 3178, 3236
BsaWI WCCGGW 2 cut(s) 142, 2877
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 6 cut(s) 214, 291, 634, 1302, 2099, 2960
Bse1I ACTGG 5 cut(s) 87, 1136, 1182, 2010, 2019
Bse3DI GCAATG 1 cut(s) 1009
BseAI TCCGGA 1 cut(s) 142
BseBI CCWGG 4 cut(s) 789, 1728, 3179, 3237
BseCI ATCGAT 1 cut(s) 43
BseDI CCNNGG 9 cut(s) 110, 276, 305, 476, 859, 2240, 2694, 3178, 3236
BseGI GGATG 5 cut(s) 359, 1036, 1132, 1870, 3274
BseLI CCNNNNNNNGG 6 cut(s) 214, 291, 634, 1302, 2099, 2960
BseMI GCAATG 1 cut(s) 1009
BseNI ACTGG 5 cut(s) 87, 1136, 1182, 2010, 2019
BseXI GCAGC 5 cut(s) 62, 155, 964, 1043, 1081
BseYI CCCAGC 2 cut(s) 796, 2344
Bsh1236I CGCG 3 cut(s) 177, 199, 2805
BshFI GGCC 6 cut(s) 787, 2079, 2245, 2398, 2508, 3050
BshNI GGYRCC 1 cut(s) 535
BshVI ATCGAT 1 cut(s) 43
BsiHKAI GWGCWC 4 cut(s) 161, 1468, 2853, 3076
BsiSI CCGG 7 cut(s) 143, 209, 232, 278, 305, 1107, 2878
BslFI GGGAC 3 cut(s) 781, 818, 2508
BslI CCNNNNNNNGG 6 cut(s) 214, 291, 634, 1302, 2099, 2960
BsmAI GTCTC 4 cut(s) 142, 1634, 1931, 3185
BsmFI GGGAC 3 cut(s) 781, 818, 2508
BsmI GAATGC 1 cut(s) 3020
BsnI GGCC 6 cut(s) 787, 2079, 2245, 2398, 2508, 3050
Bso31I GGTCTC 1 cut(s) 142
Bsp1286I GDGCHC 6 cut(s) 161, 1468, 2687, 2853, 3076, 3303
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 8 cut(s) 106, 178, 1802, 2094, 2771, 2950, 2980, 3184
Bsp1720I GCTNAGC 1 cut(s) 1741
BspANI GGCC 6 cut(s) 787, 2079, 2245, 2398, 2508, 3050
BspDI ATCGAT 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 3 cut(s) 177, 199, 2805
BspHI TCATGA 2 cut(s) 2782, 3132
BspLI GGNNCC 5 cut(s) 537, 2700, 2952, 3186, 3234
BspMI ACCTGC 1 cut(s) 3009
BspOI GCTAGC 1 cut(s) 1106
BspPI GGATC 6 cut(s) 101, 173, 2945, 2958, 3179, 3192
BspQI GCTCTTC 2 cut(s) 2172, 3291
BspT107I GGYRCC 1 cut(s) 535
BspTNI GGTCTC 1 cut(s) 142
BsrBI CCGCTC 2 cut(s) 1986, 2523
BsrDI GCAATG 1 cut(s) 1009
BsrI ACTGG 5 cut(s) 87, 1136, 1182, 2010, 2019
BssECI CCNNGG 9 cut(s) 110, 276, 305, 476, 859, 2240, 2694, 3178, 3236
BssMI GATC 8 cut(s) 106, 178, 1802, 2094, 2771, 2950, 2980, 3184
BssT1I CCWWGG 4 cut(s) 476, 859, 2240, 2694
Bst2UI CCWGG 4 cut(s) 789, 1728, 3179, 3237
Bst4CI ACNGT 9 cut(s) 383, 513, 833, 1898, 2449, 2738, 2753, 2834, 3328
Bst6I CTCTTC 4 cut(s) 309, 462, 2172, 3291
BstAPI GCANNNNNTGC 3 cut(s) 977, 2975, 3080
BstBAI YACGTR 1 cut(s) 2591
BstC8I GCNNGC 7 cut(s) 30, 175, 254, 1104, 2358, 2516, 2976
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 5 cut(s) 359, 1036, 1132, 1870, 3274
BstFNI CGCG 3 cut(s) 177, 199, 2805
BstH2I RGCGCY 1 cut(s) 2521
BstHHI GCGC 3 cut(s) 173, 1566, 2520
BstKTI GATC 8 cut(s) 109, 181, 1805, 2097, 2774, 2953, 2983, 3187
BstMAI GTCTC 4 cut(s) 142, 1634, 1931, 3185
BstMBI GATC 8 cut(s) 106, 178, 1802, 2094, 2771, 2950, 2980, 3184
BstNI CCWGG 4 cut(s) 789, 1728, 3179, 3237
BstNSI RCATGY 5 cut(s) 899, 1409, 1665, 2360, 2978
BstSCI CCNGG 9 cut(s) 207, 231, 276, 304, 787, 1106, 1726, 3177, 3235
BstSFI CTRYAG 1 cut(s) 707
BstSNI TACGTA 1 cut(s) 2591
BstUI CGCG 3 cut(s) 177, 199, 2805
BstV1I GCAGC 5 cut(s) 62, 155, 964, 1043, 1081
BstV2I GAAGAC 5 cut(s) 83, 1405, 2046, 2721, 3294
BstX2I RGATCY 4 cut(s) 1802, 2771, 2950, 3184
BstYI RGATCY 4 cut(s) 1802, 2771, 2950, 3184
Bsu15I ATCGAT 1 cut(s) 43
BsuI GTATCC 2 cut(s) 1513, 2765
BsuRI GGCC 6 cut(s) 787, 2079, 2245, 2398, 2508, 3050
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 5 cut(s) 359, 1036, 1132, 1870, 3274
BtsIMutI CAGTG 2 cut(s) 388, 2012
BveI ACCTGC 1 cut(s) 3009
Cac8I GCNNGC 7 cut(s) 30, 175, 254, 1104, 2358, 2516, 2976
CciI TCATGA 2 cut(s) 2782, 3132
CfoI GCGC 3 cut(s) 173, 1566, 2520
Cfr13I GGNCC 5 cut(s) 145, 2397, 2506, 2581, 3175
ClaI ATCGAT 1 cut(s) 43
Csp6I GTAC 8 cut(s) 418, 536, 944, 1223, 1235, 1821, 2024, 2749
CspCI CAANNNNNGTGG 4 cut(s) 262, 297, 3246, 3281
CviQI GTAC 8 cut(s) 418, 536, 944, 1223, 1235, 1821, 2024, 2749
DpnI GATC 8 cut(s) 108, 180, 1804, 2096, 2773, 2952, 2982, 3186
DpnII GATC 8 cut(s) 106, 178, 1802, 2094, 2771, 2950, 2980, 3184
DraI TTTAAA 2 cut(s) 298, 814
DraIII CACNNNGTG 1 cut(s) 3080
EaeI YGGCCR 1 cut(s) 2077
Eam1104I CTCTTC 4 cut(s) 309, 462, 2172, 3291
EarI CTCTTC 4 cut(s) 309, 462, 2172, 3291
Ecl136II GAGCTC 2 cut(s) 159, 1466
Eco105I TACGTA 1 cut(s) 2591
Eco130I CCWWGG 4 cut(s) 476, 859, 2240, 2694
Eco147I AGGCCT 1 cut(s) 787
Eco24I GRGCYC 4 cut(s) 161, 1468, 2687, 3303
Eco31I GGTCTC 1 cut(s) 142
Eco47I GGWCC 3 cut(s) 145, 2581, 3175
Eco53kI GAGCTC 2 cut(s) 159, 1466
Eco57I CTGAAG 4 cut(s) 1002, 1433, 2157, 2522
EcoICRI GAGCTC 2 cut(s) 159, 1466
EcoRI GAATTC 1 cut(s) 2369
EcoRII CCWGG 4 cut(s) 787, 1726, 3177, 3235
EcoT14I CCWWGG 4 cut(s) 476, 859, 2240, 2694
EcoT22I ATGCAT 1 cut(s) 2971
EcoT38I GRGCYC 4 cut(s) 161, 1468, 2687, 3303
ErhI CCWWGG 4 cut(s) 476, 859, 2240, 2694
FalI AAGNNNNNCTT 2 cut(s) 708, 740
FaqI GGGAC 3 cut(s) 781, 818, 2508
FauI CCCGC 4 cut(s) 182, 192, 303, 1363
FblI GTMKAC 1 cut(s) 498
Fnu4HI GCNGC 7 cut(s) 51, 169, 978, 1057, 1070, 2298, 2521
FokI GGATG 5 cut(s) 346, 1043, 1119, 1877, 3281
FriOI GRGCYC 4 cut(s) 161, 1468, 2687, 3303
Fsp4HI GCNGC 7 cut(s) 51, 169, 978, 1057, 1070, 2298, 2521
FspBI CTAG 5 cut(s) 1103, 2763, 2798, 3069, 3355
GlaI GCGC 3 cut(s) 172, 1565, 2519
GluI GCNGC 7 cut(s) 51, 169, 978, 1057, 1070, 2298, 2521
GsaI CCCAGC 2 cut(s) 800, 2348
GsuI CTGGAG 1 cut(s) 70
HaeII RGCGCY 1 cut(s) 2521
HaeIII GGCC 6 cut(s) 787, 2079, 2245, 2398, 2508, 3050
HapII CCGG 7 cut(s) 143, 209, 232, 278, 305, 1107, 2878
HhaI GCGC 3 cut(s) 173, 1566, 2520
Hin6I GCGC 3 cut(s) 171, 1564, 2518
HinP1I GCGC 3 cut(s) 171, 1564, 2518
HincII GTYRAC 3 cut(s) 205, 1495, 3096
HindII GTYRAC 3 cut(s) 205, 1495, 3096
HpaI GTTAAC 1 cut(s) 205
HpaII CCGG 7 cut(s) 143, 209, 232, 278, 305, 1107, 2878
HphI GGTGA 8 cut(s) 1070, 1205, 1425, 1667, 2000, 2191, 2975, 3023
Hpy166II GTNNAC 8 cut(s) 205, 499, 665, 1495, 2584, 2660, 2875, 3096
Hpy8I GTNNAC 8 cut(s) 205, 499, 665, 1495, 2584, 2660, 2875, 3096
Hpy99I CGWCG 1 cut(s) 3283
HpyAV CCTTC 8 cut(s) 274, 783, 795, 883, 1510, 1946, 2465, 2497
HpyCH4III ACNGT 9 cut(s) 383, 513, 833, 1898, 2449, 2738, 2753, 2834, 3328
HpyCH4IV ACGT 2 cut(s) 883, 2590
HpySE526I ACGT 2 cut(s) 883, 2590
HspAI GCGC 3 cut(s) 171, 1564, 2518
Kpn2I TCCGGA 1 cut(s) 142
KpnI GGTACC 1 cut(s) 539
KspAI GTTAAC 1 cut(s) 205
Kzo9I GATC 8 cut(s) 106, 178, 1802, 2094, 2771, 2950, 2980, 3184
LguI GCTCTTC 2 cut(s) 2172, 3291
LmnI GCTCC 4 cut(s) 444, 1420, 2848, 3102
Lsp1109I GCAGC 5 cut(s) 62, 155, 964, 1043, 1081
LweI GCATC 8 cut(s) 345, 659, 826, 1434, 1588, 2152, 2226, 3259
MaeI CTAG 5 cut(s) 1103, 2763, 2798, 3069, 3355
MaeII ACGT 2 cut(s) 883, 2590
MaeIII GTNAC 7 cut(s) 1431, 1533, 2280, 2630, 2765, 3064, 3137
MalI GATC 8 cut(s) 108, 180, 1804, 2096, 2773, 2952, 2982, 3186
MbiI CCGCTC 2 cut(s) 1986, 2523
MboI GATC 8 cut(s) 106, 178, 1802, 2094, 2771, 2950, 2980, 3184
MfeI CAATTG 1 cut(s) 2127
MflI RGATCY 4 cut(s) 1802, 2771, 2950, 3184
MhlI GDGCHC 6 cut(s) 161, 1468, 2687, 2853, 3076, 3303
MlsI TGGCCA 1 cut(s) 2079
MluNI TGGCCA 1 cut(s) 2079
MlyI GAGTC 8 cut(s) 254, 1181, 1541, 1775, 1968, 2191, 2288, 3031
MmeI TCCRAC 5 cut(s) 1155, 1351, 1899, 2808, 2978
Mox20I TGGCCA 1 cut(s) 2079
Mph1103I ATGCAT 1 cut(s) 2971
MroI TCCGGA 1 cut(s) 142
MroXI GAANNNNTTC 3 cut(s) 2118, 2320, 2790
MscI TGGCCA 1 cut(s) 2079
MslI CAYNNNNRTG 3 cut(s) 1029, 3060, 3267
Msp20I TGGCCA 1 cut(s) 2079
MspA1I CMGCKG 2 cut(s) 1370, 2300
MspI CCGG 7 cut(s) 143, 209, 232, 278, 305, 1107, 2878
MspR9I CCNGG 9 cut(s) 209, 233, 278, 306, 789, 1108, 1728, 3179, 3237
MunI CAATTG 1 cut(s) 2127
Mva1269I GAATGC 1 cut(s) 3020
MvaI CCWGG 4 cut(s) 789, 1728, 3179, 3237
MvnI CGCG 3 cut(s) 177, 199, 2805
NciI CCSGG 5 cut(s) 209, 233, 278, 306, 1108
NdeII GATC 8 cut(s) 106, 178, 1802, 2094, 2771, 2950, 2980, 3184
NheI GCTAGC 1 cut(s) 1102
NlaIV GGNNCC 5 cut(s) 537, 2700, 2952, 3186, 3234
NmuCI GTSAC 4 cut(s) 1431, 1533, 2280, 3137
NsiI ATGCAT 1 cut(s) 2971
NspI RCATGY 5 cut(s) 899, 1409, 1665, 2360, 2978
PaeI GCATGC 2 cut(s) 2360, 2978
PagI TCATGA 2 cut(s) 2782, 3132
PceI AGGCCT 1 cut(s) 787
PciI ACATGT 3 cut(s) 895, 1405, 1661
PciSI GCTCTTC 2 cut(s) 2172, 3291
PctI GAATGC 1 cut(s) 3020
PdmI GAANNNNTTC 3 cut(s) 2118, 2320, 2790
PfeI GAWTC 9 cut(s) 40, 80, 611, 1178, 2009, 2046, 2199, 2231, 3319
PkrI GCNGC 7 cut(s) 52, 170, 979, 1058, 1071, 2299, 2522
PleI GAGTC 8 cut(s) 254, 1180, 1540, 1774, 1967, 2190, 2287, 3031
PpsI GAGTC 8 cut(s) 254, 1180, 1540, 1774, 1967, 2190, 2287, 3031
Ppu21I YACGTR 1 cut(s) 2591
PscI ACATGT 3 cut(s) 895, 1405, 1661
PshBI ATTAAT 1 cut(s) 2417
PsiI TTATAA 1 cut(s) 405
Psp124BI GAGCTC 2 cut(s) 161, 1468
Psp6I CCWGG 4 cut(s) 787, 1726, 3177, 3235
PspFI CCCAGC 2 cut(s) 796, 2344
PspGI CCWGG 4 cut(s) 787, 1726, 3177, 3235
PspN4I GGNNCC 5 cut(s) 537, 2700, 2952, 3186, 3234
PspPI GGNCC 5 cut(s) 145, 2397, 2506, 2581, 3175
PsuI RGATCY 4 cut(s) 1802, 2771, 2950, 3184
RsaI GTAC 8 cut(s) 419, 537, 945, 1224, 1236, 1822, 2025, 2750
RsaNI GTAC 8 cut(s) 418, 536, 944, 1223, 1235, 1821, 2024, 2749
RseI CAYNNNNRTG 3 cut(s) 1029, 3060, 3267
SacI GAGCTC 2 cut(s) 161, 1468
SapI GCTCTTC 2 cut(s) 2172, 3291
SatI GCNGC 7 cut(s) 51, 169, 978, 1057, 1070, 2298, 2521
Sau3AI GATC 8 cut(s) 106, 178, 1802, 2094, 2771, 2950, 2980, 3184
Sau96I GGNCC 5 cut(s) 145, 2397, 2506, 2581, 3175
ScaI AGTACT 1 cut(s) 1224
SchI GAGTC 8 cut(s) 254, 1181, 1541, 1775, 1968, 2191, 2288, 3031
ScrFI CCNGG 9 cut(s) 209, 233, 278, 306, 789, 1108, 1728, 3179, 3237
SduI GDGCHC 6 cut(s) 161, 1468, 2687, 2853, 3076, 3303
SfaNI GCATC 8 cut(s) 345, 659, 826, 1434, 1588, 2152, 2226, 3259
SfcI CTRYAG 1 cut(s) 707
SinI GGWCC 3 cut(s) 145, 2581, 3175
SmiMI CAYNNNNRTG 3 cut(s) 1029, 3060, 3267
SmlI CTYRAG 3 cut(s) 430, 632, 2276
SmoI CTYRAG 3 cut(s) 430, 632, 2276
SnaBI TACGTA 1 cut(s) 2591
SpeI ACTAGT 1 cut(s) 2762
SphI GCATGC 2 cut(s) 2360, 2978
SseBI AGGCCT 1 cut(s) 787
SspI AATATT 2 cut(s) 1203, 2708
SspMI CTAG 5 cut(s) 1103, 2763, 2798, 3069, 3355
SstI GAGCTC 2 cut(s) 161, 1468
StuI AGGCCT 1 cut(s) 787
StyD4I CCNGG 9 cut(s) 207, 231, 276, 304, 787, 1106, 1726, 3177, 3235
StyI CCWWGG 4 cut(s) 476, 859, 2240, 2694
TaaI ACNGT 9 cut(s) 383, 513, 833, 1898, 2449, 2738, 2753, 2834, 3328
TaiI ACGT 2 cut(s) 886, 2593
TaqI TCGA 3 cut(s) 43, 243, 2170
TatI WGTACW 4 cut(s) 417, 943, 1222, 1234
TauI GCSGC 2 cut(s) 2300, 2523
TfiI GAWTC 9 cut(s) 40, 80, 611, 1178, 2009, 2046, 2199, 2231, 3319
TscAI CASTG 2 cut(s) 388, 2019
TseFI GTSAC 4 cut(s) 1431, 1533, 2280, 3137
TseI GCWGC 5 cut(s) 50, 168, 977, 1056, 1069
Tsp45I GTSAC 4 cut(s) 1431, 1533, 2280, 3137
TspGWI ACGGA 4 cut(s) 99, 1245, 1696, 2058
TspRI CASTG 2 cut(s) 388, 2019
VpaK11BI GGWCC 3 cut(s) 145, 2581, 3175
VspI ATTAAT 1 cut(s) 2417
XceI RCATGY 5 cut(s) 899, 1409, 1665, 2360, 2978
XcmI CCANNNNNNNNNTGG 1 cut(s) 2591
XmiI GTMKAC 1 cut(s) 498
XmnI GAANNNNTTC 3 cut(s) 2118, 2320, 2790
XspI CTAG 5 cut(s) 1103, 2763, 2798, 3069, 3355
ZrmI AGTACT 1 cut(s) 1224
Zsp2I ATGCAT 1 cut(s) 2971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.