RH7BG050300

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0006789
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG050300.1

Sequence Viewer

Length: 3753 bp
ATGGAAATCACCGAAGAACAACGAAAGCGAGCCGAGGCGAATCGATTGGCAGCCTTAGCAAAACGCAAAGCCCTCGCCGAATCCTCCAATCTTCAACAACAACACGATCCGTGGTCGCTTTTCAAATGCCAAAAGGTCTCTCCGGACCTCAATCCGAGCTCAATTCGCTGCGCCCGCGATCCAAGATCCGAAATTTCACGGGTCAACCCGGTTGTGGAGAACCAGATGTTCCGGGTCAGGCTCGAAATCTGCTCGCCGGACTCGTTCTCAGCCACCCCAGTTGCCGTTCAAGGGTTTAAATTTCCGGGGCGGGAAGAGTGCGTAAGGAGAATGAGTGATTGCTTGGCTGGTGTAATGCCATCCCACTACACGCAAAATCACAGTGGTGGAAAGGCTGGTGTTTATAAGCTGAGTGAGTACAAAGAAGTCTTGAAATGTTTAAGGAGCAACAAGGGCATTGACATTGAAGAGATACCTTGGACTACTTTCCATGTTGTAGACAGACTTTCACAGTCATATATCACAGGGAAGTGGGTACCATGCAGGCCAGAACACTTGTTGGATGATAAGGTTGATGAGTTGATCCGGAAGCTACCAAAGAGGCTATTGGATTCACTCTTGCCTTTCCAGCTTGAGGGTGTTAGATTTGGTTTGCAAAGGGGAGGACGATGTCTTATTGCAGATGAAATGGGCCTCGGAAAGACACTGCAGGCTATTGCTATTGCATGCTGCTTCATGAGTGAAGGCTCTATACTTGTTGTTTGCCCAGCTATTTTGCGTTATTCATGGGCAGAAGAATTAGAGCGTTGGCTTCCTTTTTGTTTGCCTGCTGACATCCATCTAGTTTTTGGTCATGAAAACAATCCTGCCAATTTAAAGAAATGGCCTAGAGTTGTGGTTATTTCTTATACAATGCTTCACCATTTGCAAAAGAGCATGCTAGATCGAGAATGGGCTCTCTTGATTGTTGATGAGTCCCACCATGTTCGATGTACGAAGAAAAAATCAGAACCTAGAGAGATAAAAGCTGTTCTTGATGTGGCAAGAAAGGTCAAGCGCATAGTTTTACTATCGGGGACACCTTCTTTGTCAAGGCCATTTGACATTTTTCATCAGATAGATATGTTATGGCCTGGTTTGCTGGGAAGGGACAAGTTTAAATTTGGAGAAACTTACTGTGATGCCAAATATGTTCGGGGTGTCCAAGGGAAAGTTTTTCAGGATTTCTCAAAGGGTACTCGTTTGGAGGAGTTGAACATGTTACTAACGCAAACAGTTATGATAAGACGTTTGAAGGAACATGTCCTGTCTCAGCTACCACCCAAACGCCGACAGATTATACAAGTTGTACTAAAGAAATCAGATATAGTTTCAGCAAAGGCTGCTATCAGGGTGGGAAAGAGTTGCGATGAAGATGTATCTTCAGAACATTTGGATGAACCGAATGATAGTATGGGTTGCTGCATATCAAAGCAGCTTTCTCACCAAGAATTAGGTATTGCAAAGCTAGCTGGGTTTCGTGAATGGCTCTCCATTCATCCAGTCATTGCAGAGGCAGATGAGGTTGCAAACTTGGAGTCGGATTCCAGTTCTCATAAAATGTTAATATTTGCACATCACCATAAAGTACTTGACGGAGTACAGGAGTTCATAATCCACAAAGAGATAGATTTTGTCCGAATTGATGGAAACACACTTGCCACAGATAGGCAGTTAGCTGTGCGAAAATTCCAACTATCAACTGAGGTTAAGATTGCAATAGTTGGTATTACAGCGGGAGGTGTTGGTCTTGATTTCTCATCAGCAACACATGTTGTCTTCTTGGAGCTTCCTCAGTCACCATCATTGATGCTTCAGGCTGAGGATAGAGCTCACAGGCGAGGGCAAACAAATGCAGTCAACATATACTTCTTTTCTGGGAAGGATACCATTGATGAGTCACATTGGCAATACTTGAACAGGAGTTTGCGCCGTGTTTCATCTACAACAAATGGAAAATATGATGCAATACAAGAAATAGCGGTTGAAGATGTTTCTTTTTTTGAGACATTGGGTGGAGGTGATACATGTGAAGATTATACTTTGCAAAAGTCAGAGGGTAGTGAGTTCTCCGCAGAGCTAATCAATGTTCCTGGTTCTGGCTGCTTAGCTAAGGCTATGAAGCCTTTTGAGTCAAATGATAAGTTAGTGCCAAACATTCCACAGAGATCTGAAAGACATCATGGTACTGATGGCATTTCATCACAAACTGAAAATTCTGTTATTAAGGATGATGTGGTCTCTGATTTGGATATGGATAACAGTATCTCTTTAGATGAGAAGTTGGAAACAAATGTTCCTGAGACTAAAATTAGAGAAGGGAGAGTCTATTCTTGTAAGTTAAATAAGAGCGGTGAAGATAGAGATGGACTGAAATCACTGGATAAGAAGCGGGAAGACGGAATCAAATCTCAGACAAACAAAGGACATGATGGCCAATCTGTTCAGCCGATCAAAGGACATGATGGCGAACCTGTTCAGCCAATTGAAGCTGAAGAAGGTGGCACTAACCAAGTAGATGCTCTTCGATTTGAGGTGAGTCAATATACGGGTAGAATCCACTTGTATTCTTGCATCTCAGGAGAGGATTCAAGACCAAGGCCACTTTTTGAGAATTTTAGACCAGAGGAACTTGAGTCATTAAGTTCTTCTGCCGCTGAAAGTATTAAAGGAACAGCTTCAAATTCATTCAAGGATAACCCAGCTTATTTGCATGCACTTCTTGAATTCTATAAAGAATGGAAGAAACTGAGGCCCATTGAACAAAAGAAATTAATTGGAAAGCCTTTGCAACTTCCTTTGACTGTTGAGTTGTGCTTTCTGTGTGAAGGCACTAACCACGACAATACGGGACTGCTGAAGGGCCGAAGCAAGCGCCGCGCTACACCTTTTGATGAGATTAGCAAACCCTTACCATCAAATGCTGTGTGGAAAAAGGTCCACCTACGTAGTGGCTATGGAAAAAAGGAGAAGCAATACACTCAGGGTTACACTTTGACTGATGAACCTCTCTGTAAACTCTGCCAAACACCATGCGAGAGCCCCAATGCCAAGGAACCTGAATATTTTGAAGACCTGTTCTGTAATCTTGACTGTTATGGAGAGTACCGTATCCGAACTAGTAACAGATCTATTCGTCACGAACTTTTTCAACTAGAACGCGGTGTCTGTGCAAATTGTCAGTTGGACTGTCATAAACTTGTGGAGCACATAAGACCTTTATCTGATGTAAAGCGGAGGCAGTACATTGAAAAGTTTGCACCAAGAGTGGCAAGGCTGAAGAAGTTGCTTGAGAGGCTTGTTAAGGATCCAACAGAGGGAAATGCATGGCATGCAGATCACCTTGTTCCTGTCTATCTTGGTGGAGGTGAATGCAGGCTAGAGAACATGAGGACTCTGTGTGTGGCCTGTCATTCTGATGTTACTAGAGCACAATGTGCTGAACGGCGGTCAACAAGGAGCAAGGCTAAGAAACAACTGAAAGCAATCATGAGTAACCTGAAAAACAAAGGCACAGAGATTAACCCAAAGGACCAGGGGGATCCGGAGGCAGAGGAGAATTTGAGTGATGACGAACTTTTAGTCAAGGTTCCTGGGAGCGCCTACTCATTAGCAAATCATGTGGATGCTACAACGACGAATGAAGACTTGGAAGAGCCCTCCAATAGTGAGAAGATTCCCACAGTAGAAAAATTCACATATACGCCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

1250

Amino Acids

141.19

Weight (kDa)

7.09

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 210 - 455 5e-29 SNF2-related domain
ResIII PF04851 219 - 362 1.5e-07 Type III restriction enzyme, res subunit
DEAD PF00270 220 - 364 3.4e-07 DEAD/DEAH box helicase
Helicase_C PF00271 527 - 628 2.9e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 535
AccBSI CCGCTC 1 cut(s) 2391
AccI GTMKAC 1 cut(s) 498
AccII CGCG 3 cut(s) 177, 2919, 3201
AccIII TCCGGA 3 cut(s) 142, 585, 3583
AclWI GGATC 8 cut(s) 101, 173, 180, 577, 3341, 3354, 3575, 3588
AcoI YGGCCR 1 cut(s) 2473
AcuI CTGAAG 5 cut(s) 1407, 1838, 2553, 2918, 3338
AdeI CACNNNGTG 1 cut(s) 3476
AfiI CCNNNNNNNGG 7 cut(s) 214, 291, 634, 1707, 2138, 2495, 3356
AflIII ACRYGT 4 cut(s) 1257, 1300, 1810, 2066
AhlI ACTAGT 1 cut(s) 3158
AjnI CCWGG 4 cut(s) 1132, 2131, 3573, 3631
AjuI GAANNNNNNNTTGG 2 cut(s) 3671, 3703
AleI CACNNNNGTG 1 cut(s) 384
Alw21I GWGCWC 4 cut(s) 161, 1873, 3249, 3472
Alw26I GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
AlwI GGATC 8 cut(s) 101, 173, 180, 577, 3341, 3354, 3575, 3588
Aor13HI TCCGGA 3 cut(s) 142, 585, 3583
ApeKI GCWGC 7 cut(s) 50, 168, 729, 1382, 1461, 1474, 2142
ArsI GACNNNNNNTTYG 6 cut(s) 1069, 1101, 1562, 1594, 3606, 3638
AseI ATTAAT 1 cut(s) 2813
Asp700I GAANNNNTTC 3 cut(s) 2514, 2716, 3186
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 6 cut(s) 173, 1059, 1971, 2916, 2921, 3641
AspS9I GGNCC 6 cut(s) 145, 691, 2793, 2902, 2977, 3571
AsuC2I CCSGG 3 cut(s) 209, 233, 306
AsuHPI GGTGA 9 cut(s) 911, 1475, 1610, 1830, 2072, 2405, 2587, 3371, 3419
AsuNHI GCTAGC 1 cut(s) 1507
AvaII GGWCC 3 cut(s) 145, 2977, 3571
BalI TGGCCA 1 cut(s) 2475
BamHI GGATCC 2 cut(s) 3346, 3580
BanI GGYRCC 1 cut(s) 535
BanII GRGCYC 5 cut(s) 161, 958, 1873, 3083, 3699
BarI GAAGNNNNNNTAC 4 cut(s) 735, 767, 2547, 2579
BbsI GAAGAC 4 cut(s) 1810, 2442, 3117, 3690
Bbv12I GWGCWC 4 cut(s) 161, 1873, 3249, 3472
BbvCI CCTCAGC 1 cut(s) 1860
BbvI GCAGC 7 cut(s) 62, 155, 716, 1369, 1448, 1486, 2129
BccI CCATC 9 cut(s) 367, 846, 1679, 1849, 2225, 2399, 2465, 2498, 2962
BceAI ACGGC 3 cut(s) 269, 1956, 3500
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 4 cut(s) 1134, 2133, 3575, 3633
BciVI GTATCC 2 cut(s) 1918, 3161
BcnI CCSGG 3 cut(s) 209, 233, 306
BcoDI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BcuI ACTAGT 1 cut(s) 3158
BfmI CTRYAG 1 cut(s) 707
BfoI RGCGCY 2 cut(s) 2917, 3642
BfuI GTATCC 2 cut(s) 1918, 3161
BglII AGATCT 2 cut(s) 2207, 3167
BisI GCNGC 9 cut(s) 51, 169, 730, 1383, 1462, 1475, 2143, 2694, 2917
BlpI GCTNAGC 1 cut(s) 2146
BlsI GCNGC 9 cut(s) 52, 170, 731, 1384, 1463, 1476, 2144, 2695, 2918
BmcAI AGTACT 1 cut(s) 1629
Bme1390I CCNGG 7 cut(s) 209, 233, 306, 1134, 2133, 3575, 3633
Bme18I GGWCC 3 cut(s) 145, 2977, 3571
BmgT120I GGNCC 6 cut(s) 145, 691, 2793, 2902, 2977, 3571
BmiI GGNNCC 5 cut(s) 537, 3096, 3348, 3582, 3630
BmrFI CCNGG 7 cut(s) 209, 233, 306, 1134, 2133, 3575, 3633
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 6 cut(s) 1171, 1839, 1993, 2548, 2622, 3655
BmtI GCTAGC 1 cut(s) 1511
BmuI ACTGGG 1 cut(s) 272
BpiI GAAGAC 4 cut(s) 1810, 2442, 3117, 3690
Bpu10I CCTNAGC 3 cut(s) 55, 1860, 2151
Bpu1102I GCTNAGC 1 cut(s) 2146
BpuEI CTTGAG 3 cut(s) 653, 2693, 3350
BpuMI CCSGG 3 cut(s) 209, 233, 306
Bsa29I ATCGAT 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 2987
BsaI GGTCTC 2 cut(s) 142, 2284
BsaWI WCCGGW 3 cut(s) 142, 585, 3583
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 7 cut(s) 214, 291, 634, 1707, 2138, 2495, 3356
Bse1I ACTGG 4 cut(s) 278, 1541, 1587, 2424
Bse3DI GCAATG 1 cut(s) 1545
BseAI TCCGGA 3 cut(s) 142, 585, 3583
BseBI CCWGG 4 cut(s) 1134, 2133, 3575, 3633
BseCI ATCGAT 1 cut(s) 43
BseGI GGATG 7 cut(s) 359, 568, 834, 1441, 1537, 2275, 3670
BseLI CCNNNNNNNGG 7 cut(s) 214, 291, 634, 1707, 2138, 2495, 3356
BseMI GCAATG 1 cut(s) 1545
BseNI ACTGG 4 cut(s) 278, 1541, 1587, 2424
BseRI GAGGAG 2 cut(s) 1262, 3608
BseXI GCAGC 7 cut(s) 62, 155, 716, 1369, 1448, 1486, 2129
BseYI CCCAGC 4 cut(s) 766, 1141, 1511, 2740
Bsh1236I CGCG 3 cut(s) 177, 2919, 3201
BshNI GGYRCC 1 cut(s) 535
BshVI ATCGAT 1 cut(s) 43
BsiHKAI GWGCWC 4 cut(s) 161, 1873, 3249, 3472
BsiSI CCGG 7 cut(s) 143, 209, 232, 257, 305, 586, 3584
BslFI GGGAC 4 cut(s) 961, 1090, 1163, 2904
BslI CCNNNNNNNGG 7 cut(s) 214, 291, 634, 1707, 2138, 2495, 3356
BsmAI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BsmFI GGGAC 4 cut(s) 961, 1090, 1163, 2904
BsmI GAATGC 1 cut(s) 3416
Bso31I GGTCTC 2 cut(s) 142, 2284
Bsp1286I GDGCHC 7 cut(s) 161, 958, 1873, 3083, 3249, 3472, 3699
Bsp13I TCCGGA 3 cut(s) 142, 585, 3583
Bsp1720I GCTNAGC 1 cut(s) 2146
BspDI ATCGAT 1 cut(s) 43
BspEI TCCGGA 3 cut(s) 142, 585, 3583
BspFNI CGCG 3 cut(s) 177, 2919, 3201
BspHI TCATGA 3 cut(s) 735, 853, 3528
BspLI GGNNCC 5 cut(s) 537, 3096, 3348, 3582, 3630
BspMAI CTGCAG 1 cut(s) 711
BspOI GCTAGC 1 cut(s) 1511
BspPI GGATC 8 cut(s) 101, 173, 180, 577, 3341, 3354, 3575, 3588
BspQI GCTCTTC 2 cut(s) 2568, 3687
BspT107I GGYRCC 1 cut(s) 535
BspTNI GGTCTC 2 cut(s) 142, 2284
BsrBI CCGCTC 1 cut(s) 2391
BsrDI GCAATG 1 cut(s) 1545
BsrI ACTGG 4 cut(s) 278, 1541, 1587, 2424
BssT1I CCWWGG 4 cut(s) 476, 1204, 2636, 3090
Bst2UI CCWGG 4 cut(s) 1134, 2133, 3575, 3633
Bst6I CTCTTC 4 cut(s) 309, 462, 2568, 3687
BstAPI GCANNNNNTGC 3 cut(s) 1382, 3371, 3476
BstBAI YACGTR 1 cut(s) 2987
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 7 cut(s) 359, 568, 834, 1441, 1537, 2275, 3670
BstFNI CGCG 3 cut(s) 177, 2919, 3201
BstH2I RGCGCY 2 cut(s) 2917, 3642
BstHHI GCGC 6 cut(s) 173, 1059, 1971, 2916, 2921, 3641
BstMAI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BstNI CCWGG 4 cut(s) 1134, 2133, 3575, 3633
BstNSI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2756, 3374
BstSCI CCNGG 7 cut(s) 207, 231, 304, 1132, 2131, 3573, 3631
BstSFI CTRYAG 1 cut(s) 707
BstSNI TACGTA 1 cut(s) 2987
BstUI CGCG 3 cut(s) 177, 2919, 3201
BstV1I GCAGC 7 cut(s) 62, 155, 716, 1369, 1448, 1486, 2129
BstV2I GAAGAC 4 cut(s) 1810, 2442, 3117, 3690
BstX2I RGATCY 5 cut(s) 185, 2207, 3167, 3346, 3580
BstYI RGATCY 5 cut(s) 185, 2207, 3167, 3346, 3580
Bsu15I ATCGAT 1 cut(s) 43
BsuI GTATCC 2 cut(s) 1918, 3161
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 110
BtgZI GCGATG 1 cut(s) 1422
BtsCI GGATG 7 cut(s) 359, 568, 834, 1441, 1537, 2275, 3670
BtsI GCAGTG 1 cut(s) 704
BtsIMutI CAGTG 3 cut(s) 388, 704, 2417
CciI TCATGA 3 cut(s) 735, 853, 3528
CfoI GCGC 6 cut(s) 173, 1059, 1971, 2916, 2921, 3641
Cfr13I GGNCC 6 cut(s) 145, 691, 2793, 2902, 2977, 3571
ClaI ATCGAT 1 cut(s) 43
CspCI CAANNNNNGTGG 4 cut(s) 262, 297, 3642, 3677
DraI TTTAAA 3 cut(s) 298, 876, 1159
DraIII CACNNNGTG 1 cut(s) 3476
EaeI YGGCCR 1 cut(s) 2473
Eam1104I CTCTTC 4 cut(s) 309, 462, 2568, 3687
EarI CTCTTC 4 cut(s) 309, 462, 2568, 3687
Ecl136II GAGCTC 2 cut(s) 159, 1871
Eco105I TACGTA 1 cut(s) 2987
Eco130I CCWWGG 4 cut(s) 476, 1204, 2636, 3090
Eco24I GRGCYC 5 cut(s) 161, 958, 1873, 3083, 3699
Eco31I GGTCTC 2 cut(s) 142, 2284
Eco47I GGWCC 3 cut(s) 145, 2977, 3571
Eco53kI GAGCTC 2 cut(s) 159, 1871
Eco57I CTGAAG 5 cut(s) 1407, 1838, 2553, 2918, 3338
EcoICRI GAGCTC 2 cut(s) 159, 1871
EcoRI GAATTC 1 cut(s) 2765
EcoRII CCWGG 4 cut(s) 1132, 2131, 3573, 3631
EcoT14I CCWWGG 4 cut(s) 476, 1204, 2636, 3090
EcoT22I ATGCAT 1 cut(s) 3367
EcoT38I GRGCYC 5 cut(s) 161, 958, 1873, 3083, 3699
ErhI CCWWGG 4 cut(s) 476, 1204, 2636, 3090
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 4 cut(s) 961, 1090, 1163, 2904
FauI CCCGC 4 cut(s) 182, 303, 1768, 2424
FblI GTMKAC 1 cut(s) 498
Fnu4HI GCNGC 9 cut(s) 51, 169, 730, 1383, 1462, 1475, 2143, 2694, 2917
FokI GGATG 7 cut(s) 346, 575, 821, 1448, 1524, 2282, 3677
FriOI GRGCYC 5 cut(s) 161, 958, 1873, 3083, 3699
Fsp4HI GCNGC 9 cut(s) 51, 169, 730, 1383, 1462, 1475, 2143, 2694, 2917
GlaI GCGC 6 cut(s) 172, 1058, 1970, 2915, 2920, 3640
GluI GCNGC 9 cut(s) 51, 169, 730, 1383, 1462, 1475, 2143, 2694, 2917
GsaI CCCAGC 4 cut(s) 770, 1145, 1515, 2744
HaeII RGCGCY 2 cut(s) 2917, 3642
HapII CCGG 7 cut(s) 143, 209, 232, 257, 305, 586, 3584
HhaI GCGC 6 cut(s) 173, 1059, 1971, 2916, 2921, 3641
Hin6I GCGC 6 cut(s) 171, 1057, 1969, 2914, 2919, 3639
HinP1I GCGC 6 cut(s) 171, 1057, 1969, 2914, 2919, 3639
HincII GTYRAC 3 cut(s) 205, 1900, 3492
HindII GTYRAC 3 cut(s) 205, 1900, 3492
HpaII CCGG 7 cut(s) 143, 209, 232, 257, 305, 586, 3584
HphI GGTGA 9 cut(s) 911, 1475, 1610, 1830, 2072, 2405, 2587, 3371, 3419
Hpy166II GTNNAC 6 cut(s) 205, 499, 1900, 2980, 3056, 3492
Hpy8I GTNNAC 6 cut(s) 205, 499, 1900, 2980, 3056, 3492
Hpy99I CGWCG 1 cut(s) 3679
HpyAV CCTTC 9 cut(s) 737, 1092, 1140, 1288, 1915, 2351, 2531, 2861, 2893
HpyCH4IV ACGT 2 cut(s) 1288, 2986
HpySE526I ACGT 2 cut(s) 1288, 2986
HspAI GCGC 6 cut(s) 171, 1057, 1969, 2914, 2919, 3639
Kpn2I TCCGGA 3 cut(s) 142, 585, 3583
KpnI GGTACC 1 cut(s) 539
LguI GCTCTTC 2 cut(s) 2568, 3687
LmnI GCTCC 5 cut(s) 444, 1825, 3244, 3498, 3636
Lsp1109I GCAGC 7 cut(s) 62, 155, 716, 1369, 1448, 1486, 2129
LweI GCATC 6 cut(s) 1171, 1839, 1993, 2548, 2622, 3655
MaeII ACGT 2 cut(s) 1288, 2986
MaeIII GTNAC 8 cut(s) 1260, 1836, 1938, 3026, 3161, 3176, 3460, 3533
MbiI CCGCTC 1 cut(s) 2391
MfeI CAATTG 1 cut(s) 2523
MflI RGATCY 5 cut(s) 185, 2207, 3167, 3346, 3580
MhlI GDGCHC 7 cut(s) 161, 958, 1873, 3083, 3249, 3472, 3699
MlsI TGGCCA 1 cut(s) 2475
MluNI TGGCCA 1 cut(s) 2475
MlyI GAGTC 9 cut(s) 254, 983, 1586, 1946, 2180, 2373, 2587, 2684, 3427
MmeI TCCRAC 6 cut(s) 540, 1560, 1756, 2304, 3204, 3374
Mox20I TGGCCA 1 cut(s) 2475
Mph1103I ATGCAT 1 cut(s) 3367
MroI TCCGGA 3 cut(s) 142, 585, 3583
MroXI GAANNNNTTC 3 cut(s) 2514, 2716, 3186
MscI TGGCCA 1 cut(s) 2475
MslI CAYNNNNRTG 4 cut(s) 384, 1434, 3456, 3663
Msp20I TGGCCA 1 cut(s) 2475
MspA1I CMGCKG 2 cut(s) 1775, 2696
MspI CCGG 7 cut(s) 143, 209, 232, 257, 305, 586, 3584
MspR9I CCNGG 7 cut(s) 209, 233, 306, 1134, 2133, 3575, 3633
MteI GCGCNGCGC 1 cut(s) 2917
MunI CAATTG 1 cut(s) 2523
Mva1269I GAATGC 1 cut(s) 3416
MvaI CCWGG 4 cut(s) 1134, 2133, 3575, 3633
MvnI CGCG 3 cut(s) 177, 2919, 3201
NciI CCSGG 3 cut(s) 209, 233, 306
NheI GCTAGC 1 cut(s) 1507
NlaIV GGNNCC 5 cut(s) 537, 3096, 3348, 3582, 3630
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 3 cut(s) 1836, 1938, 3176
NsiI ATGCAT 1 cut(s) 3367
NspI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2756, 3374
OliI CACNNNNGTG 1 cut(s) 384
PaeI GCATGC 4 cut(s) 729, 940, 2756, 3374
PagI TCATGA 3 cut(s) 735, 853, 3528
PciI ACATGT 4 cut(s) 1257, 1300, 1810, 2066
PciSI GCTCTTC 2 cut(s) 2568, 3687
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PctI GAATGC 1 cut(s) 3416
PdmI GAANNNNTTC 3 cut(s) 2514, 2716, 3186
PfeI GAWTC 8 cut(s) 40, 80, 611, 1583, 2442, 2595, 2627, 3715
PflFI GACNNNGTC 1 cut(s) 669
PkrI GCNGC 9 cut(s) 52, 170, 731, 1384, 1463, 1476, 2144, 2695, 2918
PleI GAGTC 9 cut(s) 254, 982, 1585, 1945, 2179, 2372, 2586, 2683, 3427
PpsI GAGTC 9 cut(s) 254, 982, 1585, 1945, 2179, 2372, 2586, 2683, 3427
Ppu21I YACGTR 1 cut(s) 2987
PscI ACATGT 4 cut(s) 1257, 1300, 1810, 2066
PshBI ATTAAT 1 cut(s) 2813
PsiI TTATAA 1 cut(s) 405
Psp124BI GAGCTC 2 cut(s) 161, 1873
Psp6I CCWGG 4 cut(s) 1132, 2131, 3573, 3631
PspFI CCCAGC 4 cut(s) 766, 1141, 1511, 2740
PspGI CCWGG 4 cut(s) 1132, 2131, 3573, 3631
PspN4I GGNNCC 5 cut(s) 537, 3096, 3348, 3582, 3630
PspPI GGNCC 6 cut(s) 145, 691, 2793, 2902, 2977, 3571
PstI CTGCAG 1 cut(s) 711
PsuI RGATCY 5 cut(s) 185, 2207, 3167, 3346, 3580
PsyI GACNNNGTC 1 cut(s) 669
RseI CAYNNNNRTG 4 cut(s) 384, 1434, 3456, 3663
SacI GAGCTC 2 cut(s) 161, 1873
SapI GCTCTTC 2 cut(s) 2568, 3687
SatI GCNGC 9 cut(s) 51, 169, 730, 1383, 1462, 1475, 2143, 2694, 2917
Sau96I GGNCC 6 cut(s) 145, 691, 2793, 2902, 2977, 3571
ScaI AGTACT 1 cut(s) 1629
SchI GAGTC 9 cut(s) 254, 983, 1586, 1946, 2180, 2373, 2587, 2684, 3427
ScrFI CCNGG 7 cut(s) 209, 233, 306, 1134, 2133, 3575, 3633
SduI GDGCHC 7 cut(s) 161, 958, 1873, 3083, 3249, 3472, 3699
SfaNI GCATC 6 cut(s) 1171, 1839, 1993, 2548, 2622, 3655
SfcI CTRYAG 1 cut(s) 707
SinI GGWCC 3 cut(s) 145, 2977, 3571
SmiMI CAYNNNNRTG 4 cut(s) 384, 1434, 3456, 3663
SmlI CTYRAG 3 cut(s) 632, 2672, 3329
SmoI CTYRAG 3 cut(s) 632, 2672, 3329
SnaBI TACGTA 1 cut(s) 2987
SpeI ACTAGT 1 cut(s) 3158
SphI GCATGC 4 cut(s) 729, 940, 2756, 3374
SspI AATATT 2 cut(s) 1608, 3104
SstI GAGCTC 2 cut(s) 161, 1873
StyD4I CCNGG 7 cut(s) 207, 231, 304, 1132, 2131, 3573, 3631
StyI CCWWGG 4 cut(s) 476, 1204, 2636, 3090
TaiI ACGT 2 cut(s) 1291, 2989
TaqI TCGA 5 cut(s) 43, 243, 946, 988, 2566
TatI WGTACW 5 cut(s) 417, 1348, 1627, 1639, 3282
TauI GCSGC 2 cut(s) 2696, 2919
TfiI GAWTC 8 cut(s) 40, 80, 611, 1583, 2442, 2595, 2627, 3715
TscAI CASTG 3 cut(s) 388, 711, 2424
TseFI GTSAC 3 cut(s) 1836, 1938, 3176
TseI GCWGC 7 cut(s) 50, 168, 729, 1382, 1461, 1474, 2142
Tsp45I GTSAC 3 cut(s) 1836, 1938, 3176
TspGWI ACGGA 3 cut(s) 99, 1650, 2454
TspRI CASTG 3 cut(s) 388, 711, 2424
Tth111I GACNNNGTC 1 cut(s) 669
VpaK11BI GGWCC 3 cut(s) 145, 2977, 3571
VspI ATTAAT 1 cut(s) 2813
XceI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2756, 3374
XcmI CCANNNNNNNNNTGG 2 cut(s) 845, 2987
XmiI GTMKAC 1 cut(s) 498
XmnI GAANNNNTTC 3 cut(s) 2514, 2716, 3186
ZrmI AGTACT 1 cut(s) 1629
Zsp2I ATGCAT 1 cut(s) 3367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.