Rh7AG050500

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006789
Physical Location & Seq
Forward (+)
3480875 .. 3491565
10691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG050500.1

Sequence Viewer

Length: 3783 bp
ATGGAAATCACCGAAGAACAGCGAAAGCGAGCCGAGGCGAATCGATTGGCAGCCTTAGCAAAACGCAAAGCCCTCGCCGAATCCTCCAATCTTCAACAACAACACGATCCGTGGTCGCTTTTCAAATGCCAAAAGGTCTCTCCGGACCTCAATCCGAGCTCAATTCGCTGCGCCCGCGATCCAAGATCCGAAATTTCACGGGTTAACCCGATTGTGGAGAACCAGATGTTCCGGGTCAGGCTCGAAATCTGCTCGCCGGACTCGTTCTCAGCCACCCCGGTTGCCGTTCAAGGGTTTAAATTTCCGGGGCGGGAAGAGTGCGCAAGGAGAATGAGTGATTGCTTGGCTGGTGTAATGCCATCCCACTACACGCAAAATCACAGTGGTGGAAAGGCTGGTGTTTATGAGCTGAGTGAGTACAAAGAAGTCTTGAAATGTTTAAGGAGCAACAAGGGCATTGACATTGAAGAGATACCTTGGACTACTTTCCATGTTGTAGACAGACTTTCACAGTCATATATCACAGGGAAGTGGGTACCATGCAGGCCAGAACACTTGTTGGATGATAAGGTTGATGAGTTGATCGGGAAGCTGCCAAAGAGGCTATTGGATTCACTCTTACCTTTCCAGCTTGAGGGTGTTAGATTTGGTTTGCAAAGGGGCGGACGATGTCTTATTGCAGATGAAATGGGCCTCGGAAAGACACTGCAGGCTATTGCTATTGCATGCTGCTTCATGAGTGAAGGCTCTATACTTGTTGTTTGCCCAGCTATTTTGCGTTATTCATGGGCAGAAGAATTAGAGCATTGGCTTCCTTTTTGTTTGCCTGCTGACATCCATCTAGTTTTTGGTCATGAGAACAATCCTGCTAATTTAAAGAAATGGCCTAGAGTTGTGGTTATTTCTTATACAATGCTTCACCATTTGCAAAAGAGCATGCTAGATCGAGAATGGGCTCTCTTGATTGTTGATGAGTCCCACCATGTACGATGTACGAAGAAAAAATCAGAACCTAGAGAGATAAAAGCTGTTCTTGATGTGGCAAGAAAGGTCAAGCGCATAGTTTTACTATCGGGGACACCTTCTTTGTCAAGGCCATTTGACATCTTTCATCAGATAGATATGTTATGGCCTGGTTTGCTGGGAAGGGACAAGTTTAAATTTGGAGAAACTTACTGTGATGCCAAATATGTTCGGGGTGTCCAAGGGAAAGTTTTTCAGGATTTCTCAAAGGGTACTCGTTTGGAGGAGTTGAACATGTTACTAACGCGAACAGTTATGATAAGACGTTTGAAGGAACATGTCCTGTCTCAGCTACCACCCAAACGCCGACAGATTATACAAGTTGTACTAAAGAAATCAGATATAGTTTCAGCAAAGGCTGCTATCAGGGTGGGAAAGAGTCACGATGAAGATGTATCTTCAGAACATTTGGATGAACTGAATGATAGTATGGGTTGCTGTATATCAAAGCAGCTTTCTCACCAAGAATTAGGTATTGCAAAGCTAGCCGGGTTTCGTGAATGGCTCTCCATTCATCCAGTCATTGCAGAGGCAGATGGGGATGCAAAATTGGAGTCGGATTCCAGTTCTCATAAAATGTTAATATTTGCACATCACCATAAAGTACTTGACGGAGTACAGGAGTTCATAATCCACAAAGAGATAGATTTTGTCCGAATTGATGGAAACACACTTGCCACAGACAGGCAGTTAGCTGTGCGAAAATTCCAACTATCAAGTGAGGTTAAGATTGCAATAGTTGGTATTACAGCGGGAGGTGTTGGTCTTGATTTCTCATCAGCTACACATGTTGTCTTCTTGGAGCTTCCTCAGTCACCATCATTGATGCTTCAGGCTGAGGATAGAGCTCACAGGCGAGGGCAAACAAATGCAGTCAACATATACTTCTTTTCTGGGAAGGATACCATTGATGAGTCACATTGGCAATACTTGAACAGGAGTTTGCGCCGTGTTTCATCTACAACAAATGGAAAATATGATGCAATACAAGAAATAGCGGTTGAAGATGTTTCTTTTTTTGAGACATTGGGTGGAGGTGATACATGTGAAGATTATACTTTGCAAAAGTCAGAGGGTAGTGAGTTCTCAGCAGAGCTAATCAAAGTTCCTGGTTCTGGCTGCTTAGCTAAGGCTATGAAGCCTTTTGAGTCAAATGATAAATTAGTGCCAAACATTCCACAGAGATCTGAAAGACATCATGGTACTGATGGCATTTCATCACAAACTGAAAATTCTGTTATTAAGGATGATGTGGTCTCTGATTTGGATATGGATAACAGTATCTCTTTAGATGAGAAGTTGGAAACAAATGTTCCTGAGACTAAAATTAGAGAAGGGACAGTAGTCTATTCTTGTAAGTTAAATAAGAGCAGTGAAGATAGAGATGGACTGGAATCACTGGATAAGGAAGACGGAATCAAATCTCAGACAAACAAAGGACATGATGGCCAATCTGTTCAGCTGATCAAAGGACATGATCGCGAACCTGTTCAGCCAATCAAAGGATATGATCGCGAACCTGTTCAGCCAATTGAAGCTGAAGAAGGTGGCACTAACCAAGTAGATGCTCTTCGATTTGAGGTGAGTCAATATACCGGTAGAATCCACTTGTATTCTTGCATCTCAGGAGAGGATTCAAGACCAAGGCCACTTTTTGAGAATTTTAGACCAGAGGAACTTGAGTCACTAAGTTCTTCTGCCGCTGAAAGTATTAAAGGAACAGTTTCAAATTCATTCAAGGATAACCCAGCTTATTTGCATGCACTTCTTGAATTCTATAAAGAATGGAAGAAACTGAGGCCCATTGAACAAAAGAAATTAATTGGAAAGCCTTTGCAACTTCCTTTGACTGTTGAGTTGTGCTTTCTGTGTGAAGGCACTAACCACGACAATACGGGACTGCTGAAGGGCCGAAGCAAGCGCCGCTCTACACCTTTTGATGAGATTAGCAAACCCCTACCATCAAATGCTGTGTGGAAAAAGGTCCACCTACGTAGTGGCTATGGAAAAAAGGAGAAGCAATACACTCAGGGTTACACTTTGACTGATGAACCTCTCTGTAAACTCTGCCAAACACCATGCGAGAGCCCCAATGCCAAGGAACCTGAATATTTTGAAGACCTGTTCTGTAATCTTGACTGTTATGGAGAGTACCGTATCCGAACTAGTAACAGATCTATTCGTCATGAACTTTTTCAACTAGAACGCGGTGTCTGTGCAAATTGTCAGTTGGACTGTCATAAACTTGTGGAGCACATAAGACCTTTATCTGATGTAAACCGGAGGCAGTATATTGAAAAGTTTGCACCAAGAGTGGCAAGGTTGAAGAAGTTGCTTGAGAGGCTTGTTAAGGATCCAACAGAGGGAAATGCATGGCATGCAGATCACCTTGTCCCTGTCTATCTTGGTGGAGGTGAATGCAGGCTAGAGAACATGAGGACTCTGTGTGTGGCCTGTCATTCTGATGTTACTAGAGCACAATGTGCTGAACGGCGGTCAACAAGGAGCAAGGCTAAGAAACAACTGAAAGCAATCATGAGTGACCTTAAAAACAAAGGCACAGAGATTAACCCAAAGGACCAGGGGGATCCAGAGGCAGAGGAGAATTTGAGTGATGACGAACTTTTAGTCAAGGTTCCTGGGAGCGCCTACTCATTAGCAAATCGTGGGGATGCTACAACGATGAATGAAGACTTGGAAGAGCCCTCCAATAGTGAGAAGATTCCCAGAGTAGAAAAATTCACGTATACGCCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

1260

Amino Acids

142.38

Weight (kDa)

6.67

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 210 - 456 7.3e-29 SNF2-related domain
ResIII PF04851 220 - 362 2e-07 Type III restriction enzyme, res subunit
DEAD PF00270 220 - 364 5.1e-07 DEAD/DEAH box helicase
Helicase_C PF00271 528 - 628 3.2e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 322
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 535
AccBSI CCGCTC 1 cut(s) 2949
AccI GTMKAC 2 cut(s) 498, 3770
AccII CGCG 5 cut(s) 177, 1270, 2505, 2538, 3231
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 9 cut(s) 175, 310, 663, 1775, 2021, 2724, 2947, 3231, 3517
AclWI GGATC 7 cut(s) 101, 173, 180, 3371, 3384, 3605, 3618
AcoI YGGCCR 1 cut(s) 2470
AcuI CTGAAG 4 cut(s) 1407, 1838, 2583, 2948
AdeI CACNNNGTG 1 cut(s) 3506
AfiI CCNNNNNNNGG 7 cut(s) 214, 291, 634, 1707, 2138, 2525, 3386
AflIII ACRYGT 4 cut(s) 1257, 1300, 1810, 2066
AgeI ACCGGT 1 cut(s) 2618
AhlI ACTAGT 1 cut(s) 3188
AjnI CCWGG 4 cut(s) 1132, 2131, 3603, 3661
AjuI GAANNNNNNNTTGG 2 cut(s) 3701, 3733
AleI CACNNNNGTG 1 cut(s) 384
Alw21I GWGCWC 4 cut(s) 161, 1873, 3279, 3502
Alw26I GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
AlwI GGATC 7 cut(s) 101, 173, 180, 3371, 3384, 3605, 3618
Aor13HI TCCGGA 1 cut(s) 142
ApeKI GCWGC 7 cut(s) 50, 168, 592, 729, 1382, 1474, 2142
ArsI GACNNNNNNTTYG 6 cut(s) 1069, 1101, 1562, 1594, 3636, 3668
AseI ATTAAT 1 cut(s) 2843
AsiGI ACCGGT 1 cut(s) 2618
Asp700I GAANNNNTTC 4 cut(s) 2511, 2544, 2746, 3216
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 6 cut(s) 173, 323, 1059, 1971, 2946, 3671
AspS9I GGNCC 6 cut(s) 145, 691, 2823, 2932, 3007, 3601
AsuC2I CCSGG 4 cut(s) 233, 278, 306, 1513
AsuHPI GGTGA 8 cut(s) 911, 1475, 1610, 1830, 2072, 2617, 3401, 3449
AsuNHI GCTAGC 1 cut(s) 1507
AvaII GGWCC 3 cut(s) 145, 3007, 3601
BalI TGGCCA 1 cut(s) 2472
BamHI GGATCC 2 cut(s) 3376, 3610
BanI GGYRCC 1 cut(s) 535
BanII GRGCYC 5 cut(s) 161, 958, 1873, 3113, 3729
BarI GAAGNNNNNNTAC 4 cut(s) 735, 767, 2577, 2609
BbsI GAAGAC 4 cut(s) 1810, 2439, 3147, 3720
Bbv12I GWGCWC 4 cut(s) 161, 1873, 3279, 3502
BbvCI CCTCAGC 1 cut(s) 1860
BbvI GCAGC 7 cut(s) 62, 155, 579, 716, 1369, 1486, 2129
BccI CCATC 9 cut(s) 367, 846, 1553, 1679, 1849, 2225, 2402, 2462, 2992
BceAI ACGGC 3 cut(s) 269, 1956, 3530
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 4 cut(s) 1134, 2133, 3605, 3663
BciVI GTATCC 2 cut(s) 1918, 3191
BclI TGATCA 1 cut(s) 2487
BcnI CCSGG 4 cut(s) 233, 278, 306, 1513
BcoDI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BcuI ACTAGT 1 cut(s) 3188
BfmI CTRYAG 1 cut(s) 707
BfoI RGCGCY 2 cut(s) 2947, 3672
BfuI GTATCC 2 cut(s) 1918, 3191
BglI GCCNNNNNGGC 1 cut(s) 601
BglII AGATCT 2 cut(s) 2207, 3197
BisI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2724, 2947
BlpI GCTNAGC 1 cut(s) 2146
BlsI GCNGC 9 cut(s) 52, 170, 594, 731, 1384, 1476, 2144, 2725, 2948
BmcAI AGTACT 1 cut(s) 1629
Bme1390I CCNGG 8 cut(s) 233, 278, 306, 1134, 1513, 2133, 3605, 3663
Bme18I GGWCC 3 cut(s) 145, 3007, 3601
BmgT120I GGNCC 6 cut(s) 145, 691, 2823, 2932, 3007, 3601
BmiI GGNNCC 5 cut(s) 537, 3126, 3378, 3612, 3660
BmrFI CCNGG 8 cut(s) 233, 278, 306, 1134, 1513, 2133, 3605, 3663
BmsI GCATC 7 cut(s) 1171, 1555, 1839, 1993, 2578, 2652, 3685
BmtI GCTAGC 1 cut(s) 1511
BoxI GACNNNNGTC 1 cut(s) 2366
BpiI GAAGAC 4 cut(s) 1810, 2439, 3147, 3720
Bpu10I CCTNAGC 3 cut(s) 55, 1860, 2151
Bpu1102I GCTNAGC 1 cut(s) 2146
BpuEI CTTGAG 3 cut(s) 653, 2723, 3380
BpuMI CCSGG 4 cut(s) 233, 278, 306, 1513
Bsa29I ATCGAT 1 cut(s) 43
BsaAI YACGTR 2 cut(s) 3017, 3768
BsaI GGTCTC 2 cut(s) 142, 2284
BsaWI WCCGGW 3 cut(s) 142, 2618, 3303
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 7 cut(s) 214, 291, 634, 1707, 2138, 2525, 3386
Bse118I RCCGGY 1 cut(s) 2618
Bse1I ACTGG 4 cut(s) 1541, 1587, 2418, 2427
Bse3DI GCAATG 1 cut(s) 1545
BseAI TCCGGA 1 cut(s) 142
BseBI CCWGG 4 cut(s) 1134, 2133, 3605, 3663
BseCI ATCGAT 1 cut(s) 43
BseGI GGATG 8 cut(s) 359, 568, 834, 1441, 1537, 1570, 2275, 3700
BseLI CCNNNNNNNGG 7 cut(s) 214, 291, 634, 1707, 2138, 2525, 3386
BseMI GCAATG 1 cut(s) 1545
BseNI ACTGG 4 cut(s) 1541, 1587, 2418, 2427
BseRI GAGGAG 2 cut(s) 1262, 3638
BseXI GCAGC 7 cut(s) 62, 155, 579, 716, 1369, 1486, 2129
BseYI CCCAGC 3 cut(s) 766, 1141, 2770
Bsh1236I CGCG 5 cut(s) 177, 1270, 2505, 2538, 3231
BshNI GGYRCC 1 cut(s) 535
BshTI ACCGGT 1 cut(s) 2618
BshVI ATCGAT 1 cut(s) 43
BsiHKAI GWGCWC 4 cut(s) 161, 1873, 3279, 3502
BsiSI CCGG 8 cut(s) 143, 232, 257, 278, 305, 1512, 2619, 3304
BslFI GGGAC 6 cut(s) 961, 1090, 1163, 2374, 2934, 3401
BslI CCNNNNNNNGG 7 cut(s) 214, 291, 634, 1707, 2138, 2525, 3386
BsmAI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BsmFI GGGAC 6 cut(s) 961, 1090, 1163, 2374, 2934, 3401
BsmI GAATGC 1 cut(s) 3446
Bso31I GGTCTC 2 cut(s) 142, 2284
Bsp1286I GDGCHC 7 cut(s) 161, 958, 1873, 3113, 3279, 3502, 3729
Bsp13I TCCGGA 1 cut(s) 142
Bsp1720I GCTNAGC 1 cut(s) 2146
Bsp68I TCGCGA 2 cut(s) 2505, 2538
BspACI CCGC 9 cut(s) 175, 310, 663, 1775, 2021, 2724, 2947, 3231, 3517
BspDI ATCGAT 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 5 cut(s) 177, 1270, 2505, 2538, 3231
BspHI TCATGA 4 cut(s) 735, 853, 3208, 3558
BspLI GGNNCC 5 cut(s) 537, 3126, 3378, 3612, 3660
BspMAI CTGCAG 1 cut(s) 711
BspOI GCTAGC 1 cut(s) 1511
BspPI GGATC 7 cut(s) 101, 173, 180, 3371, 3384, 3605, 3618
BspQI GCTCTTC 2 cut(s) 2598, 3717
BspT107I GGYRCC 1 cut(s) 535
BspTNI GGTCTC 2 cut(s) 142, 2284
BsrBI CCGCTC 1 cut(s) 2949
BsrDI GCAATG 1 cut(s) 1545
BsrFI RCCGGY 1 cut(s) 2618
BsrI ACTGG 4 cut(s) 1541, 1587, 2418, 2427
BssAI RCCGGY 1 cut(s) 2618
BssNAI GTATAC 1 cut(s) 3771
BssT1I CCWWGG 4 cut(s) 476, 1204, 2666, 3120
Bst1107I GTATAC 1 cut(s) 3771
Bst2UI CCWGG 4 cut(s) 1134, 2133, 3605, 3663
Bst6I CTCTTC 4 cut(s) 309, 462, 2598, 3717
BstAPI GCANNNNNTGC 3 cut(s) 1382, 3401, 3506
BstBAI YACGTR 2 cut(s) 3017, 3768
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 8 cut(s) 359, 568, 834, 1441, 1537, 1570, 2275, 3700
BstFNI CGCG 5 cut(s) 177, 1270, 2505, 2538, 3231
BstH2I RGCGCY 2 cut(s) 2947, 3672
BstHHI GCGC 6 cut(s) 173, 323, 1059, 1971, 2946, 3671
BstMAI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BstNI CCWGG 4 cut(s) 1134, 2133, 3605, 3663
BstNSI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2786, 3404
BstPAI GACNNNNGTC 1 cut(s) 2366
BstSCI CCNGG 8 cut(s) 231, 276, 304, 1132, 1511, 2131, 3603, 3661
BstSFI CTRYAG 1 cut(s) 707
BstSNI TACGTA 1 cut(s) 3017
BstUI CGCG 5 cut(s) 177, 1270, 2505, 2538, 3231
BstV1I GCAGC 7 cut(s) 62, 155, 579, 716, 1369, 1486, 2129
BstV2I GAAGAC 4 cut(s) 1810, 2439, 3147, 3720
BstX2I RGATCY 5 cut(s) 185, 2207, 3197, 3376, 3610
BstYI RGATCY 5 cut(s) 185, 2207, 3197, 3376, 3610
BstZ17I GTATAC 1 cut(s) 3771
Bsu15I ATCGAT 1 cut(s) 43
BsuI GTATCC 2 cut(s) 1918, 3191
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 8 cut(s) 359, 568, 834, 1441, 1537, 1570, 2275, 3700
BtsI GCAGTG 2 cut(s) 704, 2401
BtsIMutI CAGTG 4 cut(s) 388, 704, 2401, 2420
BtuMI TCGCGA 2 cut(s) 2505, 2538
CciI TCATGA 4 cut(s) 735, 853, 3208, 3558
CfoI GCGC 6 cut(s) 173, 323, 1059, 1971, 2946, 3671
Cfr10I RCCGGY 1 cut(s) 2618
Cfr13I GGNCC 6 cut(s) 145, 691, 2823, 2932, 3007, 3601
ClaI ATCGAT 1 cut(s) 43
CspAI ACCGGT 1 cut(s) 2618
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
DraI TTTAAA 3 cut(s) 298, 876, 1159
DraIII CACNNNGTG 1 cut(s) 3506
EaeI YGGCCR 1 cut(s) 2470
Eam1104I CTCTTC 4 cut(s) 309, 462, 2598, 3717
EarI CTCTTC 4 cut(s) 309, 462, 2598, 3717
EciI GGCGGA 1 cut(s) 678
Ecl136II GAGCTC 2 cut(s) 159, 1871
Eco105I TACGTA 1 cut(s) 3017
Eco130I CCWWGG 4 cut(s) 476, 1204, 2666, 3120
Eco24I GRGCYC 5 cut(s) 161, 958, 1873, 3113, 3729
Eco31I GGTCTC 2 cut(s) 142, 2284
Eco47I GGWCC 3 cut(s) 145, 3007, 3601
Eco53kI GAGCTC 2 cut(s) 159, 1871
Eco57I CTGAAG 4 cut(s) 1407, 1838, 2583, 2948
EcoICRI GAGCTC 2 cut(s) 159, 1871
EcoRI GAATTC 1 cut(s) 2795
EcoRII CCWGG 4 cut(s) 1132, 2131, 3603, 3661
EcoT14I CCWWGG 4 cut(s) 476, 1204, 2666, 3120
EcoT22I ATGCAT 1 cut(s) 3397
EcoT38I GRGCYC 5 cut(s) 161, 958, 1873, 3113, 3729
ErhI CCWWGG 4 cut(s) 476, 1204, 2666, 3120
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 6 cut(s) 961, 1090, 1163, 2374, 2934, 3401
FauI CCCGC 3 cut(s) 182, 303, 1768
FbaI TGATCA 1 cut(s) 2487
FblI GTMKAC 2 cut(s) 498, 3770
Fnu4HI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2724, 2947
FokI GGATG 8 cut(s) 346, 575, 821, 1448, 1524, 1577, 2282, 3707
FriOI GRGCYC 5 cut(s) 161, 958, 1873, 3113, 3729
Fsp4HI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2724, 2947
FspI TGCGCA 1 cut(s) 322
GlaI GCGC 6 cut(s) 172, 322, 1058, 1970, 2945, 3670
GluI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2724, 2947
GsaI CCCAGC 3 cut(s) 770, 1145, 2774
HaeII RGCGCY 2 cut(s) 2947, 3672
HapII CCGG 8 cut(s) 143, 232, 257, 278, 305, 1512, 2619, 3304
HhaI GCGC 6 cut(s) 173, 323, 1059, 1971, 2946, 3671
Hin6I GCGC 6 cut(s) 171, 321, 1057, 1969, 2944, 3669
HinP1I GCGC 6 cut(s) 171, 321, 1057, 1969, 2944, 3669
HincII GTYRAC 3 cut(s) 205, 1900, 3522
HindII GTYRAC 3 cut(s) 205, 1900, 3522
HpaI GTTAAC 1 cut(s) 205
HpaII CCGG 8 cut(s) 143, 232, 257, 278, 305, 1512, 2619, 3304
HphI GGTGA 8 cut(s) 911, 1475, 1610, 1830, 2072, 2617, 3401, 3449
Hpy166II GTNNAC 8 cut(s) 205, 499, 1900, 3010, 3086, 3301, 3522, 3771
Hpy8I GTNNAC 8 cut(s) 205, 499, 1900, 3010, 3086, 3301, 3522, 3771
HpyAV CCTTC 9 cut(s) 737, 1092, 1140, 1288, 1915, 2351, 2561, 2891, 2923
HpyCH4IV ACGT 3 cut(s) 1288, 3016, 3767
HpySE526I ACGT 3 cut(s) 1288, 3016, 3767
HspAI GCGC 6 cut(s) 171, 321, 1057, 1969, 2944, 3669
Kpn2I TCCGGA 1 cut(s) 142
KpnI GGTACC 1 cut(s) 539
Ksp22I TGATCA 1 cut(s) 2487
KspAI GTTAAC 1 cut(s) 205
LguI GCTCTTC 2 cut(s) 2598, 3717
LmnI GCTCC 5 cut(s) 444, 1825, 3274, 3528, 3666
Lsp1109I GCAGC 7 cut(s) 62, 155, 579, 716, 1369, 1486, 2129
LweI GCATC 7 cut(s) 1171, 1555, 1839, 1993, 2578, 2652, 3685
MaeII ACGT 3 cut(s) 1288, 3016, 3767
MaeIII GTNAC 9 cut(s) 1260, 1403, 1836, 1938, 2706, 3056, 3191, 3490, 3563
MbiI CCGCTC 1 cut(s) 2949
MfeI CAATTG 1 cut(s) 2553
MflI RGATCY 5 cut(s) 185, 2207, 3197, 3376, 3610
MhlI GDGCHC 7 cut(s) 161, 958, 1873, 3113, 3279, 3502, 3729
MlsI TGGCCA 1 cut(s) 2472
MluNI TGGCCA 1 cut(s) 2472
MlyI GAGTC 9 cut(s) 254, 983, 1411, 1586, 1946, 2180, 2617, 2714, 3457
MmeI TCCRAC 6 cut(s) 540, 1560, 1756, 2304, 3234, 3404
Mox20I TGGCCA 1 cut(s) 2472
Mph1103I ATGCAT 1 cut(s) 3397
MroI TCCGGA 1 cut(s) 142
MroXI GAANNNNTTC 4 cut(s) 2511, 2544, 2746, 3216
MscI TGGCCA 1 cut(s) 2472
MslI CAYNNNNRTG 3 cut(s) 384, 1434, 3486
Msp20I TGGCCA 1 cut(s) 2472
MspA1I CMGCKG 3 cut(s) 1775, 2485, 2726
MspI CCGG 8 cut(s) 143, 232, 257, 278, 305, 1512, 2619, 3304
MspR9I CCNGG 8 cut(s) 233, 278, 306, 1134, 1513, 2133, 3605, 3663
MunI CAATTG 1 cut(s) 2553
Mva1269I GAATGC 1 cut(s) 3446
MvaI CCWGG 4 cut(s) 1134, 2133, 3605, 3663
MvnI CGCG 5 cut(s) 177, 1270, 2505, 2538, 3231
NciI CCSGG 4 cut(s) 233, 278, 306, 1513
NheI GCTAGC 1 cut(s) 1507
NlaIV GGNNCC 5 cut(s) 537, 3126, 3378, 3612, 3660
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 5 cut(s) 1403, 1836, 1938, 2706, 3563
NruI TCGCGA 2 cut(s) 2505, 2538
NsbI TGCGCA 1 cut(s) 322
NsiI ATGCAT 1 cut(s) 3397
NspI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2786, 3404
OliI CACNNNNGTG 1 cut(s) 384
PaeI GCATGC 4 cut(s) 729, 940, 2786, 3404
PagI TCATGA 4 cut(s) 735, 853, 3208, 3558
PciI ACATGT 4 cut(s) 1257, 1300, 1810, 2066
PciSI GCTCTTC 2 cut(s) 2598, 3717
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PctI GAATGC 1 cut(s) 3446
PdmI GAANNNNTTC 4 cut(s) 2511, 2544, 2746, 3216
PfeI GAWTC 9 cut(s) 40, 80, 611, 1583, 2417, 2439, 2625, 2657, 3745
PflFI GACNNNGTC 1 cut(s) 669
PinAI ACCGGT 1 cut(s) 2618
PkrI GCNGC 9 cut(s) 52, 170, 594, 731, 1384, 1476, 2144, 2725, 2948
PleI GAGTC 9 cut(s) 254, 982, 1410, 1585, 1945, 2179, 2616, 2713, 3457
PpsI GAGTC 9 cut(s) 254, 982, 1410, 1585, 1945, 2179, 2616, 2713, 3457
Ppu21I YACGTR 2 cut(s) 3017, 3768
PscI ACATGT 4 cut(s) 1257, 1300, 1810, 2066
PshAI GACNNNNGTC 1 cut(s) 2366
PshBI ATTAAT 1 cut(s) 2843
Psp124BI GAGCTC 2 cut(s) 161, 1873
Psp6I CCWGG 4 cut(s) 1132, 2131, 3603, 3661
PspFI CCCAGC 3 cut(s) 766, 1141, 2770
PspGI CCWGG 4 cut(s) 1132, 2131, 3603, 3661
PspN4I GGNNCC 5 cut(s) 537, 3126, 3378, 3612, 3660
PspPI GGNCC 6 cut(s) 145, 691, 2823, 2932, 3007, 3601
PstI CTGCAG 1 cut(s) 711
PsuI RGATCY 5 cut(s) 185, 2207, 3197, 3376, 3610
PsyI GACNNNGTC 1 cut(s) 669
PvuII CAGCTG 1 cut(s) 2485
RruI TCGCGA 2 cut(s) 2505, 2538
RseI CAYNNNNRTG 3 cut(s) 384, 1434, 3486
SacI GAGCTC 2 cut(s) 161, 1873
SapI GCTCTTC 2 cut(s) 2598, 3717
SatI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2724, 2947
Sau96I GGNCC 6 cut(s) 145, 691, 2823, 2932, 3007, 3601
ScaI AGTACT 1 cut(s) 1629
SchI GAGTC 9 cut(s) 254, 983, 1411, 1586, 1946, 2180, 2617, 2714, 3457
ScrFI CCNGG 8 cut(s) 233, 278, 306, 1134, 1513, 2133, 3605, 3663
SduI GDGCHC 7 cut(s) 161, 958, 1873, 3113, 3279, 3502, 3729
SfaNI GCATC 7 cut(s) 1171, 1555, 1839, 1993, 2578, 2652, 3685
SfcI CTRYAG 1 cut(s) 707
SinI GGWCC 3 cut(s) 145, 3007, 3601
SmiMI CAYNNNNRTG 3 cut(s) 384, 1434, 3486
SmlI CTYRAG 3 cut(s) 632, 2702, 3359
SmoI CTYRAG 3 cut(s) 632, 2702, 3359
SnaBI TACGTA 1 cut(s) 3017
SpeI ACTAGT 1 cut(s) 3188
SphI GCATGC 4 cut(s) 729, 940, 2786, 3404
SsiI CCGC 9 cut(s) 175, 310, 663, 1775, 2021, 2724, 2947, 3231, 3517
SspI AATATT 2 cut(s) 1608, 3134
SstI GAGCTC 2 cut(s) 161, 1873
StyD4I CCNGG 8 cut(s) 231, 276, 304, 1132, 1511, 2131, 3603, 3661
StyI CCWWGG 4 cut(s) 476, 1204, 2666, 3120
TaiI ACGT 3 cut(s) 1291, 3019, 3770
TaqI TCGA 4 cut(s) 43, 243, 946, 2596
TatI WGTACW 4 cut(s) 417, 1348, 1627, 1639
TauI GCSGC 2 cut(s) 2726, 2949
TfiI GAWTC 9 cut(s) 40, 80, 611, 1583, 2417, 2439, 2625, 2657, 3745
TscAI CASTG 4 cut(s) 388, 711, 2401, 2427
TseFI GTSAC 5 cut(s) 1403, 1836, 1938, 2706, 3563
TseI GCWGC 7 cut(s) 50, 168, 592, 729, 1382, 1474, 2142
Tsp45I GTSAC 5 cut(s) 1403, 1836, 1938, 2706, 3563
TspGWI ACGGA 3 cut(s) 99, 1650, 2451
TspRI CASTG 4 cut(s) 388, 711, 2401, 2427
Tth111I GACNNNGTC 1 cut(s) 669
VpaK11BI GGWCC 3 cut(s) 145, 3007, 3601
VspI ATTAAT 1 cut(s) 2843
XceI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2786, 3404
XcmI CCANNNNNNNNNTGG 2 cut(s) 845, 3017
XmiI GTMKAC 2 cut(s) 498, 3770
XmnI GAANNNNTTC 4 cut(s) 2511, 2544, 2746, 3216
ZrmI AGTACT 1 cut(s) 1629
Zsp2I ATGCAT 1 cut(s) 3397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.