RLG00000005127

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006789
Physical Location & Seq
Reverse (-)
65212905 .. 65224730
11826 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005127

Sequence Viewer

Length: 4077 bp
ATGGAAATCACCGAAGAACAACGAAAGCGAGCCGAGGCGAATCGATTGGCAGCCTTAGAAAAACGCAAAGACCTCGCCGAATCCTCCAATCTTCAACAACAACACGATCCGTGGTCGCTTTTCAAATGCCGAAAGGTCTCTCCGGACCTCAATCCGAGCTCAATTCGCTGCCCCCGCGATCCAAGATCGGAAATTTCACGGGTTAACCCGGTTGTGGAGAACCAGATGTTCCGGGTCAGGCTCGAAATCTGCTCGCCGGACTCGTTCTCAGCCACCCCAGTTGCCGTTCAAGGGTTTAAATTTCCGGGGCGGGAAGAGTGCGTAAGGAGAATGAGTGATTGCTTGGCTGGTGTTATGCCATCCCACTACACGCAAAATCACAGTGGGGGAAAGGCTGGTGTTTATAAGCTGAGTGAGTGCAAAGAAGTCTTGAAATGTTTAAGGAGCAACAAGGGCATTGACATTGAAGAGATACCTTGGACTACTTTCCATGTTGTAGACAGACTTTCACAGTCATATATCACAGGGAAGTGGGTACCATGCAGGCCAGAACACTTATCGGATGATAAGGTTGATGAGTTGATCCGGAAGCTACCAAAGAGGCTATTGGATTCACTCTTGCCTTTCCAGCTTGAGGGTGTTAGATTTGGTTTGCAAAGGGGTGGACGATGTCTTATTGCAGACGAAATGGGCCTTGGAAAGACACTACAGGCTATTGCTATTGCATGCTGCTTCATGAGTGAAGGCTCTATACTTGTTGTTTGCCCAGCTATTTTGCGTTATTCTTGGGCAGAAGAATTAGAGCGTTGGCTTCCTTTTTGTTTGCCTGCTGACATCCATCTAGTTTTTGGTCATGAAAACAATCCTGCCAATTTAAAGAAATGGCCTAGAGTTGTGGTTATTTCTTATACAATGCTTCACCATTTGCAAAAGAGCATGCTAGATCAAGAATGGGCTCTCTTGATTGTTGATGAGTCCCACCATGTACGATGTACGAAGAAAAAATCAGAACCTAGAGAGATAAAAGCTGTTCTTGATGTGGCAAGAAAGGTCAAGCGCATAGTTTTACTATCGGGGACGCCTTCTTTGTCAAGGCCATTTGACATTTTTCATCAGATAGATATGTTATGGCCTGGTTTGCTGGGAAGGGACAAGTTTAAATTTGGAGAAACTTACTGTGATGCCAAATATGTTCGGGGTGTCCAAGGGAAAGTTTTTCAGGATTTCTCAAAGGGTACTCGTTTGGAGGAGTTGAACATGTTACTTACGCAAACAGTTATGATAAGACGTTTGAAGGAACATGTCCTGTCTCAGCTACCACCCAAACGCCGACAGATTATACAAGTTGTACTAAAGAAATCAGATATAGTTTCAGCAAAGGCTGCTATCAGGGTGGGAAAGAGTTGTGATGAAGATGTATCTTCAGAACATTTGGATGAACTGAATGATAGTATGGGTTGCTGCATATCAAAGCAGCTTTCTCACCAAGAATTAGGTATTGCAAAGCTAGCCGGGTTTCGTGAATGGCTCTCCATTCATCCAGTCATTGCAGAGGCAGATGGGGTTGCAAACTTGGAGTCAGATTCCAGTTCTCATAAAATGTTAATATTTGCACATCACCATAAAGTACTTGACGGAGTACAGGAGTTCATAATCCACAAAGAGATAGATTTTGTCCGAATTGATGGAAACACACTTGCCACAGATAGGCAGTTAGCTGTGCGAAAATTCCAACTATCAACTGAGGTTAAGATTGCAATAGTTGGTATTACAGCGGGAGGTGTTGGTCTTGATTTCTCATCAGCAACACACGTTGTCTTCTTGGAGCTTCCTCAGTCACCATCATTGATGCTTCAGGCTGAGGATAGAGCTCACAGGCGAGGGCAAACAAATGCAGTCAACATATACTTCTTTTCTGGGAAGGATACCGTTGATGAGTCACATTGGCAATATTTGAACAGGAGTTTGCGCTGTGTTTCATCTACAACAAATGGAAAATATGATGCAATACAAGAAATAGCGGTTGAAGATGTTTCTTTTCTTGAGACATTGGGTGGAGGTGATACATGTGAAGATTATACTTTGCAAAAGTCAGAGGGTAGTGAGTTCTCTGCAGAGCTAATCAAAGTGCCTGGTTCTGACTGCTTAGCGAAGGCTATGAAGCCTTCTGAGTCAAATGATAAGTTAGTGCCAAACATTCCTCAGAGATCTGAAAGACATCATGGTACTGATGGCATTTCATCACAAACTGAAAATTCTGTTATTAAGGATGATCTGGTCTCTGATTTGGATATGGATAACAGTATCTCTTCAGATGAGAAGTTGGAAACAAATGTTCCTGAGACTAAAATTAGAGAAGGGAGAGTCTATTCTCGTAAGTTAAATAAGAGCGGTGAAGATAGAGATGGACTGGAATCACTGGATAAGAAGCAGGAAGACGAAATCAAATCTCAGACAAACAAAGGACATGATGGCCAATCTGTTCAGCCAATCAAACGACATGATGGCAAACCTGTTCAGTCAATTGAAGCTGAAGAAGGTGGCACTAACCAAGTAGATGCTCTTCGATTTGAGGTGAGTCAATACACTGGTAGAATCCACTTGTATTCTTGCATCTCAGGAGAGGATTCAAGACCAAGGCCACTTTTTGAGAATTTTAGACCAGAGGAACTTGAGTCACTAAGTTCTTCTGCTGCTGAAAGTATTAAAGGAACAGCTTCAAATTCATTCAAGGCTAACCCAGCTTATTTGCATGCACTTCTTGCATTCAATAAAGAATGGAAGAAACTGAGAGCCATTGAACAAAAGAAATTAATTGGAAAGCCTTTGCAACTTCCTTTGACTGTTGAGTTGTGCTTTCTGTGTGAAGGCACTAACCACGACAATACGGGACTGCTGAAGGGCCGAAGCAAGCGCCGCTCTACACCTTTCGATGAGATTAGCAAACCGTTACCATCAAATGCTGCTGTGTGGAAAAAGGTCCACCTACGTAGTGGCTATGGAAAAAAGGAGAAGCAATACACTCAGGGTTACACTTTGACTGATGAACCTCTCTGTAAACTCTGCCAAACACCATGCGAGAGCCACAATGCAAAGGAACCTGAATATTTTGAAGACCTGTTCTGTAATCTTGACTGTTATGGAGAGTACCGTATCCGAACTAGTAACAGATCTATTCGTCATGAACTTTTTCAACTAGAACGCGGTGTCTGTGCAAATTGTCAGTTGGACTGTCATAAACTTGTGGAGCACATAAGACCTTTATGTGATGTAAAGCGGAGGCAGTACATTGAAAAGTTTGCACCAAGAGTGGCAAGGCTGAAGAAGTTGCTTGAGAGGCTTGTTAAGGATCCAACGGAGGGAAATGCATGGCATGCAGATCACCTTGTTCCTGTCTATCTTGGTGGAGGTGAATGCAGGCTAGAGAACATGAGGACTCTGTGTGTGGCCTGTCATTCTGATGTTACTAGAGCACAGTGTGCTGAACGGCGGTCAACAAGGAGCAAGGCTAAGAAACAACTGAAAGCAATCATGAGTAACCTGAAAAACAAAGGCACAGAGATTAACCCAAAGGACCAGCGAGATCCGGAGGCAGAGGAGAATTTGAGTGATGACGAACTTTTAGTCAAGGTTCCTGGGAGCGCCTACTCATTAGCAAATCATGTGGATGCTACAACGACGAATGAAGACTTGGAAGTGCCCTCCAATACTAAAGAAACAAGCCAACGAGGCGATGAAAACTTCCCAGCTTCGTACCAATCTCCTTCCCAACAAGATGAGCTGCAATGCCCCAGAGCATACCGAAACCCATTTGGCACCTGTATACACCATAACACCCAGAACAAGCCATTCGAGATAATGAGCTTAGCACTCGTCCAGGGCTATTCTTTCTCCTCCTCCTCCTCCTCCTCCTCCGAAGAAGAAGAAGAAGCAGAAGAAGACCAAAATGGCCTCCATTATCATAACTCATCGGACGACGACGGCGACGACGGCGTGGAGCACAAAGTAGCATCCTCCTCCGTTTATGAGTCGTCCCGACCCACCTCCTCCAATACCTCTGCCCTCCCGTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

1359

Amino Acids

152.91

Weight (kDa)

6.39

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 210 - 455 4.6e-29 SNF2-related domain
ResIII PF04851 219 - 362 1.1e-07 Type III restriction enzyme, res subunit
DEAD PF00270 220 - 364 2.6e-07 DEAD/DEAH box helicase
Helicase_C PF00271 527 - 628 4.3e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 2 cut(s) 535, 3815
AccBSI CCGCTC 2 cut(s) 2391, 2919
AccI GTMKAC 2 cut(s) 498, 3823
AccII CGCG 2 cut(s) 177, 3204
AccIII TCCGGA 3 cut(s) 142, 585, 3586
AciI CCGC 9 cut(s) 175, 310, 1775, 2021, 2391, 2917, 3204, 3277, 3490
AclWI GGATC 6 cut(s) 101, 173, 577, 3344, 3357, 3578
AcoI YGGCCR 1 cut(s) 2473
AcsI RAATTY 8 cut(s) 192, 299, 1160, 1727, 2254, 2653, 2722, 3601
AcuI CTGAAG 6 cut(s) 1407, 1838, 2295, 2553, 2918, 3341
AcyI GRCGYC 1 cut(s) 1079
AdeI CACNNNGTG 1 cut(s) 3479
AfiI CCNNNNNNNGG 5 cut(s) 214, 291, 634, 1707, 3359
AflIII ACRYGT 4 cut(s) 1257, 1300, 1810, 2066
AhlI ACTAGT 1 cut(s) 3161
AjnI CCWGG 4 cut(s) 1132, 2131, 3634, 3876
AjuI GAANNNNNNNTTGG 4 cut(s) 678, 710, 3674, 3706
Alw21I GWGCWC 5 cut(s) 161, 1873, 3252, 3475, 4002
Alw26I GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
AlwI GGATC 6 cut(s) 101, 173, 577, 3344, 3357, 3578
Aor13HI TCCGGA 3 cut(s) 142, 585, 3586
ApeKI GCWGC 9 cut(s) 50, 168, 729, 1382, 1461, 1474, 2693, 2963, 3781
ApoI RAATTY 8 cut(s) 192, 299, 1160, 1727, 2254, 2653, 2722, 3601
ArsI GACNNNNNNTTYG 6 cut(s) 1069, 1101, 1562, 1594, 3609, 3641
AseI ATTAAT 1 cut(s) 2813
Asp700I GAANNNNTTC 2 cut(s) 2716, 3189
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 4 cut(s) 1059, 1971, 2916, 3644
AspS9I GGNCC 5 cut(s) 145, 691, 2902, 2980, 3574
AsuC2I CCSGG 4 cut(s) 209, 233, 306, 1513
AsuHPI GGTGA 9 cut(s) 911, 1475, 1610, 1830, 2072, 2405, 2587, 3374, 3422
AsuNHI GCTAGC 1 cut(s) 1507
AvaII GGWCC 3 cut(s) 145, 2980, 3574
BaeGI GKGCMC 1 cut(s) 3702
BalI TGGCCA 1 cut(s) 2475
BamHI GGATCC 1 cut(s) 3349
BanI GGYRCC 2 cut(s) 535, 3815
BanII GRGCYC 3 cut(s) 161, 958, 1873
BarI GAAGNNNNNNTAC 4 cut(s) 735, 767, 2547, 2579
BbsI GAAGAC 5 cut(s) 1810, 2442, 3120, 3693, 3945
Bbv12I GWGCWC 5 cut(s) 161, 1873, 3252, 3475, 4002
BbvCI CCTCAGC 1 cut(s) 1860
BbvI GCAGC 9 cut(s) 62, 155, 716, 1369, 1448, 1486, 2680, 2950, 3768
BceAI ACGGC 4 cut(s) 269, 3503, 3997, 4006
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 4 cut(s) 1134, 2133, 3636, 3878
BciVI GTATCC 2 cut(s) 1918, 3164
BcnI CCSGG 4 cut(s) 209, 233, 306, 1513
BcoDI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BcuI ACTAGT 1 cut(s) 3161
BfaI CTAG 9 cut(s) 842, 888, 941, 1014, 1508, 3162, 3197, 3422, 3468
BfmI CTRYAG 2 cut(s) 707, 2112
BfoI RGCGCY 2 cut(s) 2917, 3645
BfuI GTATCC 2 cut(s) 1918, 3164
BglI GCCNNNNNGGC 1 cut(s) 3729
BglII AGATCT 2 cut(s) 2207, 3170
BlpI GCTNAGC 2 cut(s) 2146, 3865
BmcAI AGTACT 1 cut(s) 1629
Bme1390I CCNGG 8 cut(s) 209, 233, 306, 1134, 1513, 2133, 3636, 3878
Bme18I GGWCC 3 cut(s) 145, 2980, 3574
BmgT120I GGNCC 5 cut(s) 145, 691, 2902, 2980, 3574
BmiI GGNNCC 5 cut(s) 537, 3099, 3351, 3633, 3817
BmrFI CCNGG 8 cut(s) 209, 233, 306, 1134, 1513, 2133, 3636, 3878
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 7 cut(s) 1171, 1839, 1993, 2548, 2622, 3658, 4019
BmtI GCTAGC 1 cut(s) 1511
BmuI ACTGGG 1 cut(s) 272
BpiI GAAGAC 5 cut(s) 1810, 2442, 3120, 3693, 3945
Bpu10I CCTNAGC 1 cut(s) 1860
Bpu1102I GCTNAGC 2 cut(s) 2146, 3865
BpuEI CTTGAG 4 cut(s) 653, 2063, 2693, 3353
BpuMI CCSGG 4 cut(s) 209, 233, 306, 1513
Bsa29I ATCGAT 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 2990
BsaHI GRCGYC 1 cut(s) 1079
BsaI GGTCTC 2 cut(s) 142, 2284
BsaJI CCNNGG 9 cut(s) 33, 110, 305, 476, 694, 1204, 2636, 3635, 3877
BsaWI WCCGGW 3 cut(s) 142, 585, 3586
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 291, 634, 1707, 3359
Bse1I ACTGG 6 cut(s) 278, 1541, 1587, 2415, 2424, 2593
Bse3DI GCAATG 2 cut(s) 1545, 3791
BseAI TCCGGA 3 cut(s) 142, 585, 3586
BseBI CCWGG 4 cut(s) 1134, 2133, 3636, 3878
BseCI ATCGAT 1 cut(s) 43
BseDI CCNNGG 9 cut(s) 33, 110, 305, 476, 694, 1204, 2636, 3635, 3877
BseGI GGATG 8 cut(s) 359, 568, 834, 1441, 1537, 2275, 3673, 4010
BseLI CCNNNNNNNGG 5 cut(s) 214, 291, 634, 1707, 3359
BseMI GCAATG 2 cut(s) 1545, 3791
BseNI ACTGG 6 cut(s) 278, 1541, 1587, 2415, 2424, 2593
BseSI GKGCMC 1 cut(s) 3702
BseXI GCAGC 9 cut(s) 62, 155, 716, 1369, 1448, 1486, 2680, 2950, 3768
BseYI CCCAGC 4 cut(s) 766, 1141, 2740, 3745
Bsh1236I CGCG 2 cut(s) 177, 3204
BshNI GGYRCC 2 cut(s) 535, 3815
BshVI ATCGAT 1 cut(s) 43
BsiHKAI GWGCWC 5 cut(s) 161, 1873, 3252, 3475, 4002
BsiSI CCGG 8 cut(s) 143, 209, 232, 257, 305, 586, 1512, 3587
BslFI GGGAC 5 cut(s) 961, 1090, 1163, 2904, 4018
BslI CCNNNNNNNGG 5 cut(s) 214, 291, 634, 1707, 3359
BsmAI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BsmFI GGGAC 5 cut(s) 961, 1090, 1163, 2904, 4018
BsmI GAATGC 2 cut(s) 2765, 3419
Bso31I GGTCTC 2 cut(s) 142, 2284
Bsp1286I GDGCHC 7 cut(s) 161, 958, 1873, 3252, 3475, 3702, 4002
Bsp13I TCCGGA 3 cut(s) 142, 585, 3586
Bsp1720I GCTNAGC 2 cut(s) 2146, 3865
BspACI CCGC 9 cut(s) 175, 310, 1775, 2021, 2391, 2917, 3204, 3277, 3490
BspDI ATCGAT 1 cut(s) 43
BspEI TCCGGA 3 cut(s) 142, 585, 3586
BspFNI CGCG 2 cut(s) 177, 3204
BspHI TCATGA 4 cut(s) 735, 853, 3181, 3531
BspLI GGNNCC 5 cut(s) 537, 3099, 3351, 3633, 3817
BspMAI CTGCAG 1 cut(s) 2116
BspOI GCTAGC 1 cut(s) 1511
BspPI GGATC 6 cut(s) 101, 173, 577, 3344, 3357, 3578
BspQI GCTCTTC 1 cut(s) 2568
BspT107I GGYRCC 2 cut(s) 535, 3815
BspTNI GGTCTC 2 cut(s) 142, 2284
BsrBI CCGCTC 2 cut(s) 2391, 2919
BsrDI GCAATG 2 cut(s) 1545, 3791
BsrI ACTGG 6 cut(s) 278, 1541, 1587, 2415, 2424, 2593
BssECI CCNNGG 9 cut(s) 33, 110, 305, 476, 694, 1204, 2636, 3635, 3877
BssNAI GTATAC 1 cut(s) 3824
BssNI GRCGYC 1 cut(s) 1079
BssT1I CCWWGG 4 cut(s) 476, 694, 1204, 2636
Bst1107I GTATAC 1 cut(s) 3824
Bst2UI CCWGG 4 cut(s) 1134, 2133, 3636, 3878
Bst6I CTCTTC 4 cut(s) 309, 462, 2314, 2568
BstACI GRCGYC 1 cut(s) 1079
BstAPI GCANNNNNTGC 4 cut(s) 1382, 2762, 3374, 3479
BstBAI YACGTR 1 cut(s) 2990
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 8 cut(s) 359, 568, 834, 1441, 1537, 2275, 3673, 4010
BstFNI CGCG 2 cut(s) 177, 3204
BstH2I RGCGCY 2 cut(s) 2917, 3645
BstHHI GCGC 4 cut(s) 1059, 1971, 2916, 3644
BstMAI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BstNI CCWGG 4 cut(s) 1134, 2133, 3636, 3878
BstNSI RCATGY 7 cut(s) 729, 940, 1261, 1304, 2070, 2756, 3377
BstSCI CCNGG 8 cut(s) 207, 231, 304, 1132, 1511, 2131, 3634, 3876
BstSFI CTRYAG 2 cut(s) 707, 2112
BstSLI GKGCMC 1 cut(s) 3702
BstSNI TACGTA 1 cut(s) 2990
BstUI CGCG 2 cut(s) 177, 3204
BstV1I GCAGC 9 cut(s) 62, 155, 716, 1369, 1448, 1486, 2680, 2950, 3768
BstV2I GAAGAC 5 cut(s) 1810, 2442, 3120, 3693, 3945
BstX2I RGATCY 4 cut(s) 2207, 3170, 3349, 3583
BstYI RGATCY 4 cut(s) 2207, 3170, 3349, 3583
BstZ17I GTATAC 1 cut(s) 3824
Bsu15I ATCGAT 1 cut(s) 43
BsuI GTATCC 2 cut(s) 1918, 3164
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 110
BtgZI GCGATG 1 cut(s) 3747
BtsCI GGATG 8 cut(s) 359, 568, 834, 1441, 1537, 2275, 3673, 4010
BtsIMutI CAGTG 4 cut(s) 388, 2417, 2586, 3482
CciI TCATGA 4 cut(s) 735, 853, 3181, 3531
CfoI GCGC 4 cut(s) 1059, 1971, 2916, 3644
Cfr13I GGNCC 5 cut(s) 145, 691, 2902, 2980, 3574
ClaI ATCGAT 1 cut(s) 43
CseI GACGC 1 cut(s) 1087
CspCI CAANNNNNGTGG 4 cut(s) 262, 297, 3645, 3680
DraI TTTAAA 3 cut(s) 298, 876, 1159
DraIII CACNNNGTG 1 cut(s) 3479
EaeI YGGCCR 1 cut(s) 2473
Eam1104I CTCTTC 4 cut(s) 309, 462, 2314, 2568
EarI CTCTTC 4 cut(s) 309, 462, 2314, 2568
Ecl136II GAGCTC 2 cut(s) 159, 1871
Eco105I TACGTA 1 cut(s) 2990
Eco130I CCWWGG 4 cut(s) 476, 694, 1204, 2636
Eco24I GRGCYC 3 cut(s) 161, 958, 1873
Eco31I GGTCTC 2 cut(s) 142, 2284
Eco47I GGWCC 3 cut(s) 145, 2980, 3574
Eco53kI GAGCTC 2 cut(s) 159, 1871
Eco57I CTGAAG 6 cut(s) 1407, 1838, 2295, 2553, 2918, 3341
EcoICRI GAGCTC 2 cut(s) 159, 1871
EcoRII CCWGG 4 cut(s) 1132, 2131, 3634, 3876
EcoT14I CCWWGG 4 cut(s) 476, 694, 1204, 2636
EcoT22I ATGCAT 1 cut(s) 3370
EcoT38I GRGCYC 3 cut(s) 161, 958, 1873
ErhI CCWWGG 4 cut(s) 476, 694, 1204, 2636
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 5 cut(s) 961, 1090, 1163, 2904, 4018
FauI CCCGC 3 cut(s) 182, 303, 1768
FblI GTMKAC 2 cut(s) 498, 3823
FokI GGATG 8 cut(s) 346, 575, 821, 1448, 1524, 2282, 3680, 3997
FriOI GRGCYC 3 cut(s) 161, 958, 1873
FspBI CTAG 9 cut(s) 842, 888, 941, 1014, 1508, 3162, 3197, 3422, 3468
GlaI GCGC 4 cut(s) 1058, 1970, 2915, 3643
GsaI CCCAGC 4 cut(s) 770, 1145, 2744, 3749
HaeII RGCGCY 2 cut(s) 2917, 3645
HapII CCGG 8 cut(s) 143, 209, 232, 257, 305, 586, 1512, 3587
HgaI GACGC 1 cut(s) 1087
HhaI GCGC 4 cut(s) 1059, 1971, 2916, 3644
Hin1I GRCGYC 1 cut(s) 1079
Hin6I GCGC 4 cut(s) 1057, 1969, 2914, 3642
HinP1I GCGC 4 cut(s) 1057, 1969, 2914, 3642
HincII GTYRAC 3 cut(s) 205, 1900, 3495
HindII GTYRAC 3 cut(s) 205, 1900, 3495
HpaI GTTAAC 1 cut(s) 205
HpaII CCGG 8 cut(s) 143, 209, 232, 257, 305, 586, 1512, 3587
HphI GGTGA 9 cut(s) 911, 1475, 1610, 1830, 2072, 2405, 2587, 3374, 3422
Hpy166II GTNNAC 8 cut(s) 205, 499, 665, 1900, 2983, 3059, 3495, 3824
Hpy8I GTNNAC 8 cut(s) 205, 499, 665, 1900, 2983, 3059, 3495, 3824
Hpy99I CGWCG 5 cut(s) 3682, 3980, 3983, 3989, 3992
HpyCH4IV ACGT 3 cut(s) 1288, 1812, 2989
HpySE526I ACGT 3 cut(s) 1288, 1812, 2989
Hsp92I GRCGYC 1 cut(s) 1079
HspAI GCGC 4 cut(s) 1057, 1969, 2914, 3642
Kpn2I TCCGGA 3 cut(s) 142, 585, 3586
KpnI GGTACC 1 cut(s) 539
KspAI GTTAAC 1 cut(s) 205
LguI GCTCTTC 1 cut(s) 2568
LmnI GCTCC 6 cut(s) 444, 1825, 3247, 3501, 3639, 3997
Lsp1109I GCAGC 9 cut(s) 62, 155, 716, 1369, 1448, 1486, 2680, 2950, 3768
LweI GCATC 7 cut(s) 1171, 1839, 1993, 2548, 2622, 3658, 4019
MaeI CTAG 9 cut(s) 842, 888, 941, 1014, 1508, 3162, 3197, 3422, 3468
MaeII ACGT 3 cut(s) 1288, 1812, 2989
MaeIII GTNAC 9 cut(s) 1260, 1836, 1938, 2676, 2949, 3029, 3164, 3463, 3536
MbiI CCGCTC 2 cut(s) 2391, 2919
MfeI CAATTG 1 cut(s) 2523
MflI RGATCY 4 cut(s) 2207, 3170, 3349, 3583
MhlI GDGCHC 7 cut(s) 161, 958, 1873, 3252, 3475, 3702, 4002
MlsI TGGCCA 1 cut(s) 2475
MluNI TGGCCA 1 cut(s) 2475
MmeI TCCRAC 4 cut(s) 1756, 2304, 3207, 3377
Mox20I TGGCCA 1 cut(s) 2475
Mph1103I ATGCAT 1 cut(s) 3370
MroI TCCGGA 3 cut(s) 142, 585, 3586
MroXI GAANNNNTTC 2 cut(s) 2716, 3189
MscI TGGCCA 1 cut(s) 2475
MslI CAYNNNNRTG 3 cut(s) 1434, 3459, 3666
Msp20I TGGCCA 1 cut(s) 2475
MspA1I CMGCKG 1 cut(s) 1775
MspI CCGG 8 cut(s) 143, 209, 232, 257, 305, 586, 1512, 3587
MspR9I CCNGG 8 cut(s) 209, 233, 306, 1134, 1513, 2133, 3636, 3878
MunI CAATTG 1 cut(s) 2523
Mva1269I GAATGC 2 cut(s) 2765, 3419
MvaI CCWGG 4 cut(s) 1134, 2133, 3636, 3878
MvnI CGCG 2 cut(s) 177, 3204
NciI CCSGG 4 cut(s) 209, 233, 306, 1513
NheI GCTAGC 1 cut(s) 1507
NlaIV GGNNCC 5 cut(s) 537, 3099, 3351, 3633, 3817
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 3 cut(s) 1836, 1938, 2676
NsiI ATGCAT 1 cut(s) 3370
NspI RCATGY 7 cut(s) 729, 940, 1261, 1304, 2070, 2756, 3377
PaeI GCATGC 4 cut(s) 729, 940, 2756, 3377
PagI TCATGA 4 cut(s) 735, 853, 3181, 3531
PciI ACATGT 3 cut(s) 1257, 1300, 2066
PciSI GCTCTTC 1 cut(s) 2568
PcsI WCGNNNNNNNCGW 3 cut(s) 260, 3981, 3984
PctI GAATGC 2 cut(s) 2765, 3419
PdmI GAANNNNTTC 2 cut(s) 2716, 3189
PfeI GAWTC 7 cut(s) 40, 80, 611, 1583, 2414, 2595, 2627
PflFI GACNNNGTC 1 cut(s) 669
Ppu21I YACGTR 1 cut(s) 2990
PscI ACATGT 3 cut(s) 1257, 1300, 2066
PshBI ATTAAT 1 cut(s) 2813
PsiI TTATAA 1 cut(s) 405
Psp124BI GAGCTC 2 cut(s) 161, 1873
Psp6I CCWGG 4 cut(s) 1132, 2131, 3634, 3876
PspFI CCCAGC 4 cut(s) 766, 1141, 2740, 3745
PspGI CCWGG 4 cut(s) 1132, 2131, 3634, 3876
PspN4I GGNNCC 5 cut(s) 537, 3099, 3351, 3633, 3817
PspPI GGNCC 5 cut(s) 145, 691, 2902, 2980, 3574
PstI CTGCAG 1 cut(s) 2116
PsuI RGATCY 4 cut(s) 2207, 3170, 3349, 3583
PsyI GACNNNGTC 1 cut(s) 669
RseI CAYNNNNRTG 3 cut(s) 1434, 3459, 3666
SacI GAGCTC 2 cut(s) 161, 1873
SapI GCTCTTC 1 cut(s) 2568
Sau96I GGNCC 5 cut(s) 145, 691, 2902, 2980, 3574
ScaI AGTACT 1 cut(s) 1629
ScrFI CCNGG 8 cut(s) 209, 233, 306, 1134, 1513, 2133, 3636, 3878
SduI GDGCHC 7 cut(s) 161, 958, 1873, 3252, 3475, 3702, 4002
SfaNI GCATC 7 cut(s) 1171, 1839, 1993, 2548, 2622, 3658, 4019
SfcI CTRYAG 2 cut(s) 707, 2112
SinI GGWCC 3 cut(s) 145, 2980, 3574
SmiMI CAYNNNNRTG 3 cut(s) 1434, 3459, 3666
SmlI CTYRAG 4 cut(s) 632, 2042, 2672, 3332
SmoI CTYRAG 4 cut(s) 632, 2042, 2672, 3332
SnaBI TACGTA 1 cut(s) 2990
SpeI ACTAGT 1 cut(s) 3161
SphI GCATGC 4 cut(s) 729, 940, 2756, 3377
SsiI CCGC 9 cut(s) 175, 310, 1775, 2021, 2391, 2917, 3204, 3277, 3490
SspI AATATT 3 cut(s) 1608, 1952, 3107
SspMI CTAG 9 cut(s) 842, 888, 941, 1014, 1508, 3162, 3197, 3422, 3468
SstI GAGCTC 2 cut(s) 161, 1873
StyD4I CCNGG 8 cut(s) 207, 231, 304, 1132, 1511, 2131, 3634, 3876
StyI CCWWGG 4 cut(s) 476, 694, 1204, 2636
TaiI ACGT 3 cut(s) 1291, 1815, 2992
TaqI TCGA 5 cut(s) 43, 243, 2566, 2931, 3852
TatI WGTACW 4 cut(s) 1348, 1627, 1639, 3285
TauI GCSGC 1 cut(s) 2919
TfiI GAWTC 7 cut(s) 40, 80, 611, 1583, 2414, 2595, 2627
TscAI CASTG 4 cut(s) 388, 2424, 2593, 3482
TseFI GTSAC 3 cut(s) 1836, 1938, 2676
TseI GCWGC 9 cut(s) 50, 168, 729, 1382, 1461, 1474, 2693, 2963, 3781
Tsp45I GTSAC 3 cut(s) 1836, 1938, 2676
TspGWI ACGGA 4 cut(s) 99, 1650, 3371, 4009
TspRI CASTG 4 cut(s) 388, 2424, 2593, 3482
Tth111I GACNNNGTC 1 cut(s) 669
VpaK11BI GGWCC 3 cut(s) 145, 2980, 3574
VspI ATTAAT 1 cut(s) 2813
XapI RAATTY 8 cut(s) 192, 299, 1160, 1727, 2254, 2653, 2722, 3601
XceI RCATGY 7 cut(s) 729, 940, 1261, 1304, 2070, 2756, 3377
XcmI CCANNNNNNNNNTGG 2 cut(s) 845, 2990
XmiI GTMKAC 2 cut(s) 498, 3823
XmnI GAANNNNTTC 2 cut(s) 2716, 3189
XspI CTAG 9 cut(s) 842, 888, 941, 1014, 1508, 3162, 3197, 3422, 3468
ZrmI AGTACT 1 cut(s) 1629
Zsp2I ATGCAT 1 cut(s) 3370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.