MD14G1208200.v1.1

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006789
Physical Location & Seq
Forward (+)
29490510 .. 29499160
8651 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1208200.v1.1.491

Sequence Viewer

Length: 3666 bp
ATGGAGATCACAGAAGAACAAAGAAGACGAGCCGAAGCGAATCGATTAGTAGCACTAGCGAAACGCAAAGCGCTGATCGAATCCGAATCCTCCAATCTTCAACAACAGCAGCAAAACCCATGGTCACTCTTCAAGTGCCGAAAGATTTCTCCCGACCTCACCCCAAGCTCCACGCATTGCATCCCCGATCCGAGACCCGAATTTTCCCGGGTCAAACCGGTTCCGGATATTCAATTTCCAGCGAGGTTCCGGGTCAGGCTCGAAATCTGCTCCCCGGACTCCTTCTCAGCCACCCCAGTTGCCGTAAAAGGGTTTGCATTTCCGGGGCAGGAGGAGTGCTTAAGGAGACTAAGTGATTGCTTGGCTAATGTCATGCCATCCCATTACACGCAAAATCATGGTGGTGGAAAGGCCGGTGTTTATAAGCTGAGTGATTACAGTGCGGTCTTGAAATGTCTAAGAAGCAGCAAGAGCATTGACACTGAAGAAATACCTTGGGGCACTCTTAATGTTGTAGAGAGACTTTCACATTCTTTTGTTGCGGGAAAATGGATACCATGCAGGCCAGAACACTTGTCAGACGAAATGGTTGATGAGTTCGTTGGGAAGCTACCGAAGAAGCTGTTAGATGCACTTTTGCCTTTCCAACATGAGGGTGTTAGATTTGGTTTGCAAAGAGGTGGACGATGTCTTATTGCAGACGAAATGGGCCTTGGAAAGACACTCCAGGCTATTGCTATTGCAAGCTGTTTCATGCATGAAGGTTCCATACTTGTTGTTTGCCCTGCCATTTTACGTTACTCTTGGGCAGAAGAACTAGAGCGGTGGCTTCCGTTTTGCTTGCCAGCTGACATCCATCTAGTATTTGGTCATGAAAACAATCCTGCCAATTTAAAGAGATGGCCTAGAGTTGTGGTTATTTCTTACACAATGCTCCACCGGTTGCATAAGAGCATGCTTGAACGAGAATGGGCTCTCTTAGTTGTTGATGAATCCCACCACGTACGATGTACCAAGAAATCATCAGAACCTAGAGAGATAAAAGCTGTTCTTGACATTGCAACAAAGGTCAAGCGCATAGTTTTACTATCTGGAACACCTTCTTTGTCAAGGCCGTATGACATTTTTCATCAGATAGATATGTTATGGCCTGGTTTGCTGGGAAAAGACAAGTATGACTTTGCCAAAACTTACTGTGATGTCAAATATATCCACGGTGTTCAAGGGAAAACTTTTCAGGATTTCTCTAAGGGCACTCGTTTGGAGGAGTTGAACATGTTACTGAAGCAAACGGTTATGATAAGACGCTTGAAGGAACATGTCCTGGTTCAGCTACCACCCAAACGCCGCCAGATTATACAAGTAGTACTGAAGAAATCAGATATAGCTTCAGCAATAGCTGCTGTCAGGGTGGGAAGAAGTGCCACTAAGAATGCATCTTCAGAAAATTTGGATGAACCAAATGATAGTGAGTGTTGCTGCAGATCAGGGCAACTTTCCTACCAAGAATTAGGTATTGCAAAGCTAGCCGGTTTTCGGGAATGGCTCTCCATTCATCCACTCATTGGAGAATCAGATGGTGTTGTAAACTTGGAATCAGATTCCAGTTCTCACAAAATGTTAATATTTGCGCATCACCATAAAGTACTTGACGGATTACAGGAGTTCATATGTGAGAAAGGGATTACTTTTGTCCGAATTGATGGAAATACACTTGCTGCAGATAGGCAGGAAGCTGTACGATCATTCCAACTATCTACCGAGGTCAAGATTGCAATAATTGGTATTACTGCTGGAGGTGTTGGCCTTGATTTCTCATCAGCAAAGCATGTTGTGTTCTTGGAGCTTCCTCAGTCTCCATCGTTGATGCTTCAGGCTGAGGATAGAGCTCACAGGAGAGGGCAAACAAATGCAGTCAACATATACATATTTTGCGGAAAGGATACTATAGACGAGTCACATTGGCAAAACTTGAACAAGAGTTTGCACCGTGTTTCATCTACAACGAACGGGAAGTATGATGCAATACAAGAGATACCGGTCGAAGGTATTTCTTTCTTTGAGACGTCAGGTGGAGCTGATACTTGTGAAGATCATATGTGGCAAAAGACAGTGGGTGATGAGTTCTCTGGAGAGTCAATAAAATTGTCTAACTTTGGCTGCTTAGCCAAATATATGATACCGGCGTATGATAAATTGGTTGCAAACATTCTACATGGATCTGCACAGCACAATGATAGTGATGGAATTTCATCCCAAACGCACAGTAGTACCAAGGCTCAAGTGGTTTTTGATTGCAATGGGGACAAGGGATGTTCAGAAGAGAACTTGGAAGGAGATGTTTCTACGAGCAGTGAAGACAAAGATGGACTTAAAAAGGAACACCAAAACAACTGTCAAGCAACCAAATCAAAGGACGCCGAACCAGTTCAGCCAATGGAAGCTAAAGAAAGTAGTACCAACCAAGTAGATGCTTTGCGATTTGAGGTGAGCCAATACACTGGTAGAATCCACTTGTATTCTTGCATCTCAAGAGAGGATTCAAGACCAAGGCCACTTTTTGAGAATTTTAGACCAGAAGAACTCGAGGCATTGAATTCTACTGCTGCTGACAATATTAAAGGAACTTCTTTCAAATTTTTGAAGGAGAATCCAGGTTATTTGCATGCTCTTCTTGCATTCATCAAAGAATGGAAGAAATTGAGGCCCATCGAACAAAAAAAATTACTTGGAAAGCCTTTGCAGCTTCCTTTGACTATTGAGTTGTGCTACCTGTGCGAAGGCATTAACCACGACATCAGGGGACTACTGAAAGGTGGAAGCAAGCGCCGCTCTACACCTTTTGTTGAGATCAGCAAACCTTTACCATCAAACACTGTGTGGAAAAAAGTCCACCTACGTAGTGGCTATGGAAAAAGGGAGAAGGAATACACTCAGGGTTTTACTCTGACTGATGATCCTTTGTGCAAACTGTGTCAAACACCATGCACGGGCCATAATGCCAAGACACCTGAATATTTTGAAGATCTGTTCTGCCAGCTTAGCTGCTACGAAGAATACCGCATCCGAACAAGTAACAGATCTCTTCGTCATGAGCTTTTTCAACTGGAACGTGGTGTCTGCACAATTTGCCAATTAGATTGTCATAAACTTGTTGAGCACCTAAGACCGTTATCCTTGGCAATGAGGAGGCAGTATATTGAAAATTTTGCGCCACGAGTGGCAACTCTGAAGAAACCGCTTGATAGGCTTGTTAAGGATCCGAAAGAGGGCAATGCATGGCATGCAGACCACTTTGTTCCTGTCTATCGAGGTGGAGGTGAATGCAGGCTCGAGAACATGAGAACACTTTGTGTGGCCTGTCATCGCGATGTTACTAAAGCACAATGTGCTGAACGGCTGTCAACAAGGATCCAGGCTAAGAAAAAACTGAAAGCAATCATGAACGACATTAAAAAGAAAGCTACGGAGACTAATCTAGCGGGCCCGGAACATATAGAGATTATGCAAGAGGATGCAATTGAGGATGAGCTTTTCGTCAAGGTTCCTGGGAGCGCCTACTCATTGGAAAATCGCTTGGATGCTACAACTACAGGCGAAGACTTGGACGAGCCCTCCAGTAGCAACAAGGTTCCAACGGTAGATAGCCATCTGTTAGGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

1222

Amino Acids

137.52

Weight (kDa)

7.9

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 215 - 460 1.7e-30 SNF2-related domain
ResIII PF04851 225 - 368 1.7e-07 Type III restriction enzyme, res subunit
DEAD PF00270 226 - 371 1.4e-06 DEAD/DEAH box helicase
Helicase_C PF00271 533 - 634 3.2e-17 Helicase conserved C-terminal domain
HNH PF01844 1090 - 1126 9.2e-06 HNH endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 423
AatII GACGTC 1 cut(s) 2069
Acc16I TGCGCA 1 cut(s) 1632
AccB7I CCANNNNNTGG 1 cut(s) 1565
AccBSI CCGCTC 2 cut(s) 823, 2832
AccII CGCG 1 cut(s) 3369
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 9 cut(s) 443, 542, 823, 1348, 1935, 2830, 3061, 3239, 3482
AclWI GGATC 7 cut(s) 182, 2227, 2951, 3254, 3267, 3406, 3419
AcsI RAATTY 7 cut(s) 200, 1447, 2247, 2566, 2596, 2636, 3205
AcuI CTGAAG 7 cut(s) 504, 1304, 1374, 1391, 1425, 1856, 3251
AcyI GRCGYC 2 cut(s) 2066, 2418
AdeI CACNNNGTG 3 cut(s) 2880, 3353, 3389
AfaI GTAC 7 cut(s) 1005, 1012, 1368, 1647, 1740, 2272, 2458
AfeI AGCGCT 1 cut(s) 72
AfiI CCNNNNNNNGG 7 cut(s) 309, 652, 1536, 1565, 2045, 2990, 3269
AflII CTTAAG 1 cut(s) 340
AflIII ACRYGT 2 cut(s) 1275, 1318
AgeI ACCGGT 3 cut(s) 217, 939, 2038
AjnI CCWGG 6 cut(s) 726, 1150, 1323, 2653, 3414, 3547
AjuI GAANNNNNNNTTGG 2 cut(s) 696, 728
Alw21I GWGCWC 2 cut(s) 1891, 3162
Alw26I GTCTC 6 cut(s) 187, 340, 514, 1860, 2057, 3464
AlwI GGATC 7 cut(s) 182, 2227, 2951, 3254, 3267, 3406, 3419
Ama87I CYCGRG 3 cut(s) 207, 2585, 3332
Aor13HI TCCGGA 1 cut(s) 223
Aor51HI AGCGCT 1 cut(s) 72
ApaI GGGCCC 1 cut(s) 3488
ApeKI GCWGC 9 cut(s) 109, 465, 1400, 1479, 1718, 2160, 2606, 2743, 3045
ApoI RAATTY 7 cut(s) 200, 1447, 2247, 2566, 2596, 2636, 3205
ArsI GACNNNNNNTTYG 2 cut(s) 2380, 2412
AsiGI ACCGGT 3 cut(s) 217, 939, 2038
Asp700I GAANNNNTTC 3 cut(s) 145, 1099, 2427
AspLEI GCGC 6 cut(s) 73, 1077, 1633, 2829, 3214, 3557
AspS9I GGNCC 5 cut(s) 709, 2706, 2992, 3484, 3485
AsuC2I CCSGG 6 cut(s) 208, 209, 251, 275, 324, 3488
AsuHPI GGTGA 5 cut(s) 151, 1628, 2129, 2500, 3332
AsuNHI GCTAGC 1 cut(s) 1525
AvaI CYCGRG 3 cut(s) 207, 2585, 3332
BaeGI GKGCMC 3 cut(s) 503, 1256, 3488
BaeI ACNNNNGTAYC 4 cut(s) 2171, 2204, 2254, 2287
BamHI GGATCC 2 cut(s) 3259, 3411
BanII GRGCYC 4 cut(s) 976, 1891, 3488, 3615
BarI GAAGNNNNNNTAC 2 cut(s) 753, 785
BauI CACGAG 1 cut(s) 3216
BbsI GAAGAC 3 cut(s) 31, 2364, 3606
Bbv12I GWGCWC 2 cut(s) 1891, 3162
BbvCI CCTCAGC 1 cut(s) 1878
BbvI GCAGC 9 cut(s) 121, 477, 1387, 1466, 1705, 2147, 2593, 2755, 3032
BceAI ACGGC 3 cut(s) 287, 1099, 3413
BciT130I CCWGG 6 cut(s) 728, 1152, 1325, 2655, 3416, 3549
BciVI GTATCC 2 cut(s) 546, 1936
BcnI CCSGG 6 cut(s) 208, 209, 251, 275, 324, 3488
BcoDI GTCTC 6 cut(s) 187, 340, 514, 1860, 2057, 3464
BfaI CTAG 7 cut(s) 56, 818, 860, 906, 1032, 1526, 3479
BfmI CTRYAG 4 cut(s) 1480, 1719, 1947, 3591
BfoI RGCGCY 3 cut(s) 74, 2830, 3558
BfrI CTTAAG 1 cut(s) 340
BfuI GTATCC 2 cut(s) 546, 1936
BglII AGATCT 2 cut(s) 3025, 3080
BlpI GCTNAGC 2 cut(s) 2164, 3041
BmcAI AGTACT 2 cut(s) 1368, 1647
BmeT110I CYCGRG 3 cut(s) 207, 2585, 3332
BmgT120I GGNCC 5 cut(s) 709, 2706, 2992, 3484, 3485
BmiI GGNNCC 8 cut(s) 222, 248, 766, 3261, 3413, 3486, 3546, 3633
BmrI ACTGGG 1 cut(s) 290
BmtI GCTAGC 1 cut(s) 1529
BmuI ACTGGG 1 cut(s) 290
BpiI GAAGAC 3 cut(s) 31, 2364, 3606
BpmI CTGGAG 4 cut(s) 710, 1815, 2151, 3601
Bpu10I CCTNAGC 1 cut(s) 1878
Bpu1102I GCTNAGC 2 cut(s) 2164, 3041
BpuEI CTTGAG 2 cut(s) 2265, 2515
BpuMI CCSGG 6 cut(s) 208, 209, 251, 275, 324, 3488
Bsa29I ATCGAT 1 cut(s) 43
BsaAI YACGTR 2 cut(s) 1003, 2900
BsaBI GATNNNNATC 1 cut(s) 3648
BsaHI GRCGYC 2 cut(s) 2066, 2418
BsaI GGTCTC 1 cut(s) 187
BsaWI WCCGGW 4 cut(s) 217, 223, 939, 2038
BsaXI ACNNNNNCTCC 4 cut(s) 337, 367, 3599, 3629
Bsc4I CCNNNNNNNGG 7 cut(s) 309, 652, 1536, 1565, 2045, 2990, 3269
Bse118I RCCGGY 6 cut(s) 217, 413, 939, 1529, 2038, 2182
Bse1I ACTGG 6 cut(s) 296, 1605, 2426, 2506, 3111, 3618
Bse3DI GCAATG 5 cut(s) 175, 1056, 2305, 3189, 3280
Bse8I GATNNNNATC 1 cut(s) 3648
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 6 cut(s) 728, 1152, 1325, 2655, 3416, 3549
BseCI ATCGAT 1 cut(s) 43
BseJI GATNNNNATC 1 cut(s) 3648
BseLI CCNNNNNNNGG 7 cut(s) 309, 652, 1536, 1565, 2045, 2990, 3269
BseMI GCAATG 5 cut(s) 175, 1056, 2305, 3189, 3280
BseMII CTCAG 5 cut(s) 300, 419, 1865, 1869, 2948
BseNI ACTGG 6 cut(s) 296, 1605, 2426, 2506, 3111, 3618
BseRI GAGGAG 3 cut(s) 347, 1280, 3202
BseSI GKGCMC 3 cut(s) 503, 1256, 3488
BseXI GCAGC 9 cut(s) 121, 477, 1387, 1466, 1705, 2147, 2593, 2755, 3032
BseYI CCCAGC 1 cut(s) 1159
BsgI GTGCAG 2 cut(s) 2208, 3106
Bsh1236I CGCG 1 cut(s) 3369
Bsh1285I CGRYCG 1 cut(s) 2043
BshTI ACCGGT 3 cut(s) 217, 939, 2038
BshVI ATCGAT 1 cut(s) 43
BsiEI CGRYCG 1 cut(s) 2043
BsiHKAI GWGCWC 2 cut(s) 1891, 3162
BsiHKCI CYCGRG 3 cut(s) 207, 2585, 3332
BsiWI CGTACG 1 cut(s) 1003
BslFI GGGAC 2 cut(s) 2318, 2817
BslI CCNNNNNNNGG 7 cut(s) 309, 652, 1536, 1565, 2045, 2990, 3269
BsmAI GTCTC 6 cut(s) 187, 340, 514, 1860, 2057, 3464
BsmBI CGTCTC 1 cut(s) 2057
BsmFI GGGAC 2 cut(s) 2318, 2817
BsmI GAATGC 3 cut(s) 1438, 2678, 3329
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 3 cut(s) 207, 2585, 3332
Bsp120I GGGCCC 1 cut(s) 3484
Bsp1286I GDGCHC 7 cut(s) 503, 976, 1256, 1891, 3162, 3488, 3615
Bsp13I TCCGGA 1 cut(s) 223
Bsp1720I GCTNAGC 2 cut(s) 2164, 3041
Bsp19I CCATGG 1 cut(s) 119
Bsp68I TCGCGA 1 cut(s) 3369
BspACI CCGC 9 cut(s) 443, 542, 823, 1348, 1935, 2830, 3061, 3239, 3482
BspCNI CTCAG 5 cut(s) 299, 420, 1864, 1870, 2947
BspDI ATCGAT 1 cut(s) 43
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 1 cut(s) 3369
BspHI TCATGA 3 cut(s) 871, 3091, 3441
BspLI GGNNCC 8 cut(s) 222, 248, 766, 3261, 3413, 3486, 3546, 3633
BspMAI CTGCAG 2 cut(s) 1484, 1723
BspOI GCTAGC 1 cut(s) 1529
BspPI GGATC 7 cut(s) 182, 2227, 2951, 3254, 3267, 3406, 3419
BspQI GCTCTTC 1 cut(s) 2676
BspTI CTTAAG 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 187
BsrBI CCGCTC 2 cut(s) 823, 2832
BsrDI GCAATG 5 cut(s) 175, 1056, 2305, 3189, 3280
BsrFI RCCGGY 6 cut(s) 217, 413, 939, 1529, 2038, 2182
BsrI ACTGG 6 cut(s) 296, 1605, 2426, 2506, 3111, 3618
BssAI RCCGGY 6 cut(s) 217, 413, 939, 1529, 2038, 2182
BssNI GRCGYC 2 cut(s) 2066, 2418
BssSI CACGAG 1 cut(s) 3216
BssT1I CCWWGG 6 cut(s) 119, 494, 712, 2274, 2549, 3177
Bst2BI CACGAG 1 cut(s) 3216
Bst2UI CCWGG 6 cut(s) 728, 1152, 1325, 2655, 3416, 3549
Bst6I CTCTTC 4 cut(s) 134, 2316, 2676, 3090
BstACI GRCGYC 2 cut(s) 2066, 2418
BstAFI CTTAAG 1 cut(s) 340
BstAPI GCANNNNNTGC 4 cut(s) 1400, 3129, 3284, 3389
BstBAI YACGTR 2 cut(s) 1003, 2900
BstDSI CCRYGG 2 cut(s) 119, 1213
BstFNI CGCG 1 cut(s) 3369
BstH2I RGCGCY 3 cut(s) 74, 2830, 3558
BstHHI GCGC 6 cut(s) 73, 1077, 1633, 2829, 3214, 3557
BstMAI GTCTC 6 cut(s) 187, 340, 514, 1860, 2057, 3464
BstMCI CGRYCG 1 cut(s) 2043
BstNI CCWGG 6 cut(s) 728, 1152, 1325, 2655, 3416, 3549
BstNSI RCATGY 6 cut(s) 958, 1279, 1322, 1832, 2669, 3287
BstSFI CTRYAG 4 cut(s) 1480, 1719, 1947, 3591
BstSLI GKGCMC 3 cut(s) 503, 1256, 3488
BstSNI TACGTA 1 cut(s) 2900
BstUI CGCG 1 cut(s) 3369
BstV1I GCAGC 9 cut(s) 121, 477, 1387, 1466, 1705, 2147, 2593, 2755, 3032
BstV2I GAAGAC 3 cut(s) 31, 2364, 3606
BstX2I RGATCY 5 cut(s) 2219, 3025, 3080, 3259, 3411
BstXI CCANNNNNNTGG 1 cut(s) 2501
BstYI RGATCY 5 cut(s) 2219, 3025, 3080, 3259, 3411
Bsu15I ATCGAT 1 cut(s) 43
BsuI GTATCC 2 cut(s) 546, 1936
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 2 cut(s) 119, 1213
BtgZI GCGATG 2 cut(s) 3350, 3384
BtsI GCAGTG 1 cut(s) 2359
BtsIMutI CAGTG 6 cut(s) 445, 480, 2118, 2359, 2499, 2874
BtuMI TCGCGA 1 cut(s) 3369
CciI TCATGA 3 cut(s) 871, 3091, 3441
CfoI GCGC 6 cut(s) 73, 1077, 1633, 2829, 3214, 3557
Cfr10I RCCGGY 6 cut(s) 217, 413, 939, 1529, 2038, 2182
Cfr13I GGNCC 5 cut(s) 709, 2706, 2992, 3484, 3485
Cfr9I CCCGGG 1 cut(s) 207
ClaI ATCGAT 1 cut(s) 43
CseI GACGC 2 cut(s) 1314, 2426
Csp6I GTAC 7 cut(s) 1004, 1011, 1367, 1646, 1739, 2271, 2457
CspAI ACCGGT 3 cut(s) 217, 939, 2038
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviQI GTAC 7 cut(s) 1004, 1011, 1367, 1646, 1739, 2271, 2457
DraI TTTAAA 1 cut(s) 894
DraIII CACNNNGTG 3 cut(s) 2880, 3353, 3389
Eam1104I CTCTTC 4 cut(s) 134, 2316, 2676, 3090
EarI CTCTTC 4 cut(s) 134, 2316, 2676, 3090
Ecl136II GAGCTC 1 cut(s) 1889
Eco105I TACGTA 1 cut(s) 2900
Eco130I CCWWGG 6 cut(s) 119, 494, 712, 2274, 2549, 3177
Eco24I GRGCYC 4 cut(s) 976, 1891, 3488, 3615
Eco31I GGTCTC 1 cut(s) 187
Eco47III AGCGCT 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 1889
Eco57I CTGAAG 7 cut(s) 504, 1304, 1374, 1391, 1425, 1856, 3251
Eco88I CYCGRG 3 cut(s) 207, 2585, 3332
EcoICRI GAGCTC 1 cut(s) 1889
EcoRI GAATTC 1 cut(s) 2596
EcoRII CCWGG 6 cut(s) 726, 1150, 1323, 2653, 3414, 3547
EcoT14I CCWWGG 6 cut(s) 119, 494, 712, 2274, 2549, 3177
EcoT22I ATGCAT 3 cut(s) 759, 1438, 3280
EcoT38I GRGCYC 4 cut(s) 976, 1891, 3488, 3615
ErhI CCWWGG 6 cut(s) 119, 494, 712, 2274, 2549, 3177
Esp3I CGTCTC 1 cut(s) 2057
FalI AAGNNNNNCTT 6 cut(s) 1035, 1067, 1163, 1195, 2355, 2387
FaqI GGGAC 2 cut(s) 2318, 2817
FauI CCCGC 2 cut(s) 535, 3475
FauNDI CATATG 2 cut(s) 1670, 2097
FriOI GRGCYC 4 cut(s) 976, 1891, 3488, 3615
FspBI CTAG 7 cut(s) 56, 818, 860, 906, 1032, 1526, 3479
FspI TGCGCA 1 cut(s) 1632
GlaI GCGC 6 cut(s) 72, 1076, 1632, 2828, 3213, 3556
GsaI CCCAGC 1 cut(s) 1163
GsuI CTGGAG 4 cut(s) 710, 1815, 2151, 3601
HaeII RGCGCY 3 cut(s) 74, 2830, 3558
HgaI GACGC 2 cut(s) 1314, 2426
HhaI GCGC 6 cut(s) 73, 1077, 1633, 2829, 3214, 3557
Hin1I GRCGYC 2 cut(s) 2066, 2418
Hin6I GCGC 6 cut(s) 71, 1075, 1631, 2827, 3212, 3555
HinP1I GCGC 6 cut(s) 71, 1075, 1631, 2827, 3212, 3555
HincII GTYRAC 2 cut(s) 1918, 3405
HindII GTYRAC 2 cut(s) 1918, 3405
HphI GGTGA 5 cut(s) 151, 1628, 2129, 2500, 3332
Hpy166II GTNNAC 5 cut(s) 683, 1588, 1918, 2893, 3405
Hpy8I GTNNAC 5 cut(s) 683, 1588, 1918, 2893, 3405
HpyAV CCTTC 9 cut(s) 292, 755, 1110, 1306, 2039, 2327, 2638, 2774, 2917
HpyCH4IV ACGT 5 cut(s) 796, 1002, 2066, 2899, 3112
HpySE526I ACGT 5 cut(s) 796, 1002, 2066, 2899, 3112
Hsp92I GRCGYC 2 cut(s) 2066, 2418
HspAI GCGC 6 cut(s) 71, 1075, 1631, 2827, 3212, 3555
Kpn2I TCCGGA 1 cut(s) 223
LguI GCTCTTC 1 cut(s) 2676
LmnI GCTCC 6 cut(s) 173, 275, 939, 1843, 2075, 3552
Lsp1109I GCAGC 9 cut(s) 121, 477, 1387, 1466, 1705, 2147, 2593, 2755, 3032
MaeI CTAG 7 cut(s) 56, 818, 860, 906, 1032, 1526, 3479
MaeII ACGT 5 cut(s) 796, 1002, 2066, 2899, 3112
MaeIII GTNAC 6 cut(s) 123, 797, 1278, 1956, 3074, 3373
MbiI CCGCTC 2 cut(s) 823, 2832
MfeI CAATTG 1 cut(s) 3519
MflI RGATCY 5 cut(s) 2219, 3025, 3080, 3259, 3411
MhlI GDGCHC 7 cut(s) 503, 976, 1256, 1891, 3162, 3488, 3615
MlyI GAGTC 3 cut(s) 272, 1964, 2144
MmeI TCCRAC 3 cut(s) 670, 1774, 3659
Mph1103I ATGCAT 3 cut(s) 759, 1438, 3280
MroI TCCGGA 1 cut(s) 223
MroXI GAANNNNTTC 3 cut(s) 145, 1099, 2427
MseI TTAA 9 cut(s) 341, 507, 893, 1622, 2373, 2619, 2787, 3255, 3453
MslI CAYNNNNRTG 3 cut(s) 402, 654, 3369
MspA1I CMGCKG 1 cut(s) 848
MspCI CTTAAG 1 cut(s) 340
MunI CAATTG 1 cut(s) 3519
Mva1269I GAATGC 3 cut(s) 1438, 2678, 3329
MvaI CCWGG 6 cut(s) 728, 1152, 1325, 2655, 3416, 3549
MvnI CGCG 1 cut(s) 3369
NciI CCSGG 6 cut(s) 208, 209, 251, 275, 324, 3488
NcoI CCATGG 1 cut(s) 119
NdeI CATATG 2 cut(s) 1670, 2097
NheI GCTAGC 1 cut(s) 1525
NlaIV GGNNCC 8 cut(s) 222, 248, 766, 3261, 3413, 3486, 3546, 3633
NmuCI GTSAC 2 cut(s) 123, 1956
NruI TCGCGA 1 cut(s) 3369
NsbI TGCGCA 1 cut(s) 1632
NsiI ATGCAT 3 cut(s) 759, 1438, 3280
NspI RCATGY 6 cut(s) 958, 1279, 1322, 1832, 2669, 3287
PaeI GCATGC 3 cut(s) 958, 2669, 3287
PaeR7I CTCGAG 2 cut(s) 2585, 3332
PagI TCATGA 3 cut(s) 871, 3091, 3441
PciI ACATGT 2 cut(s) 1275, 1318
PciSI GCTCTTC 1 cut(s) 2676
PctI GAATGC 3 cut(s) 1438, 2678, 3329
PdmI GAANNNNTTC 3 cut(s) 145, 1099, 2427
Pfl23II CGTACG 1 cut(s) 1003
PflFI GACNNNGTC 1 cut(s) 687
PflMI CCANNNNNTGG 1 cut(s) 1565
PinAI ACCGGT 3 cut(s) 217, 939, 2038
PleI GAGTC 3 cut(s) 272, 1963, 2143
PpsI GAGTC 3 cut(s) 272, 1963, 2143
Ppu21I YACGTR 2 cut(s) 1003, 2900
PscI ACATGT 2 cut(s) 1275, 1318
PsiI TTATAA 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 1891
Psp6I CCWGG 6 cut(s) 726, 1150, 1323, 2653, 3414, 3547
PspFI CCCAGC 1 cut(s) 1159
PspGI CCWGG 6 cut(s) 726, 1150, 1323, 2653, 3414, 3547
PspLI CGTACG 1 cut(s) 1003
PspN4I GGNNCC 8 cut(s) 222, 248, 766, 3261, 3413, 3486, 3546, 3633
PspOMI GGGCCC 1 cut(s) 3484
PspPI GGNCC 5 cut(s) 709, 2706, 2992, 3484, 3485
PspXI VCTCGAGB 1 cut(s) 2585
PstI CTGCAG 2 cut(s) 1484, 1723
PsuI RGATCY 5 cut(s) 2219, 3025, 3080, 3259, 3411
PsyI GACNNNGTC 1 cut(s) 687
PvuII CAGCTG 1 cut(s) 848
RruI TCGCGA 1 cut(s) 3369
RsaI GTAC 7 cut(s) 1005, 1012, 1368, 1647, 1740, 2272, 2458
RsaNI GTAC 7 cut(s) 1004, 1011, 1367, 1646, 1739, 2271, 2457
RseI CAYNNNNRTG 3 cut(s) 402, 654, 3369
SacI GAGCTC 1 cut(s) 1891
SapI GCTCTTC 1 cut(s) 2676
SaqAI TTAA 9 cut(s) 341, 507, 893, 1622, 2373, 2619, 2787, 3255, 3453
Sau96I GGNCC 5 cut(s) 709, 2706, 2992, 3484, 3485
ScaI AGTACT 2 cut(s) 1368, 1647
SchI GAGTC 3 cut(s) 272, 1964, 2144
SduI GDGCHC 7 cut(s) 503, 976, 1256, 1891, 3162, 3488, 3615
SfcI CTRYAG 4 cut(s) 1480, 1719, 1947, 3591
Sfr274I CTCGAG 2 cut(s) 2585, 3332
SlaI CTCGAG 2 cut(s) 2585, 3332
SmaI CCCGGG 1 cut(s) 209
SmiMI CAYNNNNRTG 3 cut(s) 402, 654, 3369
SmlI CTYRAG 5 cut(s) 340, 2280, 2530, 2585, 3332
SmoI CTYRAG 5 cut(s) 340, 2280, 2530, 2585, 3332
SnaBI TACGTA 1 cut(s) 2900
SphI GCATGC 3 cut(s) 958, 2669, 3287
SsiI CCGC 9 cut(s) 443, 542, 823, 1348, 1935, 2830, 3061, 3239, 3482
SspI AATATT 3 cut(s) 1626, 2617, 3017
SspMI CTAG 7 cut(s) 56, 818, 860, 906, 1032, 1526, 3479
SstI GAGCTC 1 cut(s) 1891
StyI CCWWGG 6 cut(s) 119, 494, 712, 2274, 2549, 3177
TaiI ACGT 5 cut(s) 799, 1005, 2069, 2902, 3115
TaqI TCGA 8 cut(s) 43, 78, 261, 2043, 2586, 2712, 3310, 3333
TatI WGTACW 2 cut(s) 1366, 1645
TauI GCSGC 2 cut(s) 1350, 2832
Tru1I TTAA 9 cut(s) 341, 507, 893, 1622, 2373, 2619, 2787, 3255, 3453
Tru9I TTAA 9 cut(s) 341, 507, 893, 1622, 2373, 2619, 2787, 3255, 3453
TscAI CASTG 6 cut(s) 445, 487, 2118, 2359, 2506, 2881
TseFI GTSAC 2 cut(s) 123, 1956
TseI GCWGC 9 cut(s) 109, 465, 1400, 1479, 1718, 2160, 2606, 2743, 3045
Tsp45I GTSAC 2 cut(s) 123, 1956
TspGWI ACGGA 3 cut(s) 822, 1668, 3482
TspMI CCCGGG 1 cut(s) 207
TspRI CASTG 6 cut(s) 445, 487, 2118, 2359, 2506, 2881
Tth111I GACNNNGTC 1 cut(s) 687
Van91I CCANNNNNTGG 1 cut(s) 1565
Vha464I CTTAAG 1 cut(s) 340
XapI RAATTY 7 cut(s) 200, 1447, 2247, 2566, 2596, 2636, 3205
XceI RCATGY 6 cut(s) 958, 1279, 1322, 1832, 2669, 3287
XcmI CCANNNNNNNNNTGG 3 cut(s) 863, 2281, 2900
XhoI CTCGAG 2 cut(s) 2585, 3332
XmaI CCCGGG 1 cut(s) 207
XmnI GAANNNNTTC 3 cut(s) 145, 1099, 2427
XspI CTAG 7 cut(s) 56, 818, 860, 906, 1032, 1526, 3479
ZraI GACGTC 1 cut(s) 2067
ZrmI AGTACT 2 cut(s) 1368, 1647
Zsp2I ATGCAT 3 cut(s) 759, 1438, 3280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.