Rw7G004130

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006789
Physical Location & Seq
Forward (+)
3439569 .. 3449976
10408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004130.1

Sequence Viewer

Length: 3723 bp
ATGGAAATCACCGAAGAACAGAGAAAGCGAGCCGAGGCGAACCGATTGGCAGCCTTAGCAAAACGCAAAGCCCTCGCCGAATCCTCCAATCTTCAACAACAACACGATCCGTGGTCGCTTTTCAAATGCCAAAAGGTCTCTCCGGACCTCAATCCGAGCTCAATTCGCTGCGCCAGCGATCCAAGATCCGGAATTTCACGGGTTAACCCGGTTGTGGAGAACCAGATGTTCCGGGTCAGGCTCGAAATCTGCTCGCCGGACTCGTTCTCAGCCACCCCGGTTGCCGTTCAAGGGTTTAAATTTCCGGGGCGGGAAGAGTGCGTAAGGAGAATGAGTGATTGCTTGGCTGGTGTAATGCCATCCCACTACACGCAAAATCACAGTGGTGGAAAGGCTGGTGTTTATAAGCTGAGTGAGTACAAAGAAGTCTTGAAATGTTTAAGGAGCAACAAGGGCATTGACATTGAAGAGATACCTTGGACTACTTTCCATGTTGTAGACAGACTTTCACAGTCATATATCACAGGGAAGTGGGTACCATGCAGGCCAGAACACTTGTTGGATGATAAGGTTGATGAGTTGATCGGGAAGCTGCCAAAGAGGCTATTGGATTCACTCTTACCTTTCCAGCTTGAGGGTGTTAGATTTGGTTTGCAAAGGGGCGGACGATGTCTTATTGCAGATGAAATGGGCCTCGGAAAGACACTGCAGGCTATTGCTATTGCATGCTGCTTCATGAGTGAAGGCTCTATACTTGTTGTTTGCCCAGCTATTTTGCGTTATTCATGGGCAGAAGAATTAGAGCATTGGCTTCCTTTTTGTTTGCCTGCTGACATCCATCTAGTTTTTGGTCATGAGAACAATCCTGCTAATTTAAAGAAATGGCCTAGAGTTGTGGTTATTTCTTATACAATGCTTCACCATTTGCAAAAGAGCATGCTAGATCGAGAATGGGCTCTCTTGATTGTTGATGAGTCCCACCATGTACGATGTACGAAGAAAAAATCAGAACCTAGAGAGATAAAAGCTGTTCTTGATGTGGCAAGAAAGGTCAAGCGCATAGTTTTACTATCGGGGACACCTTCTTTGTCAAGGCCATTTGACATCTTTCATCAGATAGATATGTTATGGCCTGGTTTGCTGGGAAGGGACAAGTTTAAATTTGGAGAAACTTACTGTGATGCCAAATATGTTCGGGGTGTCCAAGGGAAAGTTTTTCAGGATTTCTCAAAGGGTACTCGTTTGGAGGAGTTGAACATGTTACTAACGCGAACAGTTATGATAAGACGTTTGAAGGAACATGTCCTGTCTCAGCTACCACCCAAACGCCGACAGATTATACAAGTTGTACTAAAGAATTCAGATATAGTTTCAGCAAAGGCTGCTATCAGGGTGGGAAAGAGTCACGATGAAGATGTATCTTCAGAACATTTGGATGAACTGAATGATAGTATGGGTTGCTGTATATCAAAGCAGCTTTCTCACCAAGAATTAGGTATTGCAAAGCTAGCCGGGTTTCGTGAATGGCTCTCCATTCATCCAGTCATTGCAGAGGCAGATGGGGTTGCAAAATTGGAGTCGGATTCCAGTTCTCATAAAATGTTAATATTTGCACATCACCATAAAGTACTTGACGGAGTACAGGAGTTCATAATCCACAAAGAGATAGATTTTGTCCGAATTGATGGAAACACACTTGCCACAGACAGGCAGTTGGCTGTGCGAAAATTCCAACTATCAAGTGAGGTTAAGATTGCAATAGTTGGTATTACAGCGGGAGGTGTTGGTCTTGATTTCTCATCAGCAACACATGTTGTCTTCTTGGAGCTTCCTCAGTCACCATCATTGATGCTTCAGGCTGAGGATAGAGCTCACAGGCGAGGGCAAACAAATGCAGTCAACATATACTTCTTTTCTGGGAAGGATACCATTGATGAGTCACATTGGCAATACTTGAACAGGAGTTTGCGCCGTGTTTCATCTACAACAAATGGAAAATATGATGCAATACAAGAAATAGCGGTTGAAGATGTTTCTTTTTTTGAGACATTGGGTGGAGGTGATACATGTGAAGATTATACTTTGCAAAAGTCAGAGGGTAGTGAGTTCTCAGCAGAGCTAATCAAAGTTCCTGGTTCTGGCTGCTTAGCTAAGGCTATGAAGCCTTTTGAGTCAAATGATAAATTAGTGCCAAACATTCCACAGAGATCTGAAAGACATCATGGTACTGATGGCATTTCATCACAAACTGAAAATTCTGTTATTAAGGATGATGTGGTCTCTGATTTGGATATGGATAACAGTATCTCTTTAGATGAGAAGTTGGAAACAAATGTTCCTGAGACTAAAATTAGAGAAGGGACAGTAGTCTATTCTTGTAAGTTAAATAAGAGCAGTGAAGATAGAGATGGACTGGAATCACTGGATAAGCTGATCAAAGGACATGATCGCGAACCTGTTCAGCCAATCAAAGGACATGATCGCGAACCTGTTCAGCCAATTGAAGCTGAAGAAGGTGGCACTAACCAAGTAGATGCTCTTCGATTTGAGGTGAGTCAATATACCGGTAGAATCCACTTGTATTCTTGCATCTCAGGAGAGGATTCAAGACCAAGGCCACTTTTTGAGAATTTTAGACCAGAGGAACTTGAGTCACTAAGTTCTTCTGCCGCTGAAAGTATTAAAGGAACAGCTTCAAATTCATTCAAGGATAACCCAGCTTATTTGCATGCACTTCTTGAATTCTATAAAGAATGGAAGAAACTGAGGCCCATTGAACAAAAGAAATTAATTGGAAAGCCTTTGCAACTTCCTTTGACTGTTGAGTTGTGCTTTCTGTGTGAAGGCACTAACCACGACAATACGGGACTGCTGAAGGGCCGAAGCAAGCGCCGCTCTACACCTTTTGATGAGATTAGCAAACCCCTACCATCAAATGCTGTGTGGAAAAAGGTCCACCTACGTAGTGGCTATGGAAAAAAGGAGAAGCAATACACTCAGGGTTACACTTTGACTGATGAACCTCTCTGTAAACTCTGCCAAACACCATGCGAGAGCCCCAATGCCAAGGAACCTGAATATTTTGAAGACCTGTTCTGTAATCTTGACTGTTATGGAGAGTACCGTATCCGAACTAGTAACAGATCTATTCGTCACGAACTTTTTCAACTAGAACGCGGTGTCTGTGCAAATTGTCAGTTGGACTGTCATAAACTTGTGGAGCACATAAGACCTTTATCTGATGTAAACCGGAGGCAGTATATTGAAAAGTTTGCACCAAGAGTGGCAAGGTTGAAGAAGTTGCTTGAGAGGCTTGTTAAGGATCCAACAGAGGGAAATGCATGGCATGCAGATCACCTTGTCCCTGTCTATCTTGGTGGAGGTGAATGCAGGCTAGAGAACATGAGGACTCTGTGTGTGGCCTGTCATTCTGATGTTACTAGAGCACAATGTGCTGAACGGCGGTCAACAAGGAGCAAGGCTAAGAAACAACTGAAAGCAATCATGAGTGACCTTAAAAACAAAGGCACAGAGATTAACCCAAAGGGGGATCCAGAGGCAGGGGAGAATTTGAGTGATGACGAACTTTTAGTCAAGGTTCCTGGGAGCGCCTACTCATTAGCAAATCGTGGGGATGCTACAACGATGAATGAAGACTTGGAAGAGCCCTCCAATAGTGAGAAGATTCCCAGAGTAGAAAAATTCACGTATACGCCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

1240

Amino Acids

139.94

Weight (kDa)

7.1

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 210 - 455 1.4e-28 SNF2-related domain
ResIII PF04851 220 - 362 2e-07 Type III restriction enzyme, res subunit
DEAD PF00270 220 - 364 5e-07 DEAD/DEAH box helicase
Helicase_C PF00271 527 - 628 2.5e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 535
AccBSI CCGCTC 1 cut(s) 2895
AccI GTMKAC 2 cut(s) 498, 3710
AccII CGCG 4 cut(s) 1270, 2451, 2484, 3177
AccIII TCCGGA 2 cut(s) 142, 188
AciI CCGC 8 cut(s) 310, 663, 1775, 2021, 2670, 2893, 3177, 3463
AclWI GGATC 7 cut(s) 101, 173, 180, 3317, 3330, 3545, 3558
AcuI CTGAAG 4 cut(s) 1407, 1838, 2529, 2894
AdeI CACNNNGTG 1 cut(s) 3452
AflIII ACRYGT 4 cut(s) 1257, 1300, 1810, 2066
AgeI ACCGGT 1 cut(s) 2564
AhlI ACTAGT 1 cut(s) 3134
AjnI CCWGG 3 cut(s) 1132, 2131, 3601
AjuI GAANNNNNNNTTGG 2 cut(s) 3641, 3673
AleI CACNNNNGTG 1 cut(s) 384
Alw21I GWGCWC 4 cut(s) 161, 1873, 3225, 3448
Alw26I GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
AlwI GGATC 7 cut(s) 101, 173, 180, 3317, 3330, 3545, 3558
Aor13HI TCCGGA 2 cut(s) 142, 188
AoxI GGCC 9 cut(s) 545, 691, 884, 1094, 1130, 2615, 2768, 2878, 3420
ApeKI GCWGC 7 cut(s) 50, 168, 592, 729, 1382, 1474, 2142
ArsI GACNNNNNNTTYG 6 cut(s) 1069, 1101, 1562, 1594, 3576, 3608
AseI ATTAAT 1 cut(s) 2789
AsiGI ACCGGT 1 cut(s) 2564
Asp700I GAANNNNTTC 4 cut(s) 2457, 2490, 2692, 3162
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 5 cut(s) 173, 1059, 1971, 2892, 3611
AspS9I GGNCC 5 cut(s) 145, 691, 2769, 2878, 2953
AsuC2I CCSGG 5 cut(s) 209, 233, 278, 306, 1513
AsuHPI GGTGA 8 cut(s) 911, 1475, 1610, 1830, 2072, 2563, 3347, 3395
AsuNHI GCTAGC 1 cut(s) 1507
AvaII GGWCC 2 cut(s) 145, 2953
BamHI GGATCC 2 cut(s) 3322, 3550
BanI GGYRCC 1 cut(s) 535
BanII GRGCYC 5 cut(s) 161, 958, 1873, 3059, 3669
BarI GAAGNNNNNNTAC 4 cut(s) 735, 767, 2523, 2555
BbsI GAAGAC 3 cut(s) 1810, 3093, 3660
Bbv12I GWGCWC 4 cut(s) 161, 1873, 3225, 3448
BbvCI CCTCAGC 1 cut(s) 1860
BbvI GCAGC 7 cut(s) 62, 155, 579, 716, 1369, 1486, 2129
BccI CCATC 8 cut(s) 367, 846, 1553, 1679, 1849, 2225, 2402, 2938
BceAI ACGGC 3 cut(s) 269, 1956, 3476
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 3 cut(s) 1134, 2133, 3603
BciVI GTATCC 2 cut(s) 1918, 3137
BclI TGATCA 1 cut(s) 2433
BcnI CCSGG 5 cut(s) 209, 233, 278, 306, 1513
BcoDI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BcuI ACTAGT 1 cut(s) 3134
BfmI CTRYAG 1 cut(s) 707
BfoI RGCGCY 2 cut(s) 2893, 3612
BfuI GTATCC 2 cut(s) 1918, 3137
BglI GCCNNNNNGGC 1 cut(s) 601
BglII AGATCT 2 cut(s) 2207, 3143
BisI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2670, 2893
BlpI GCTNAGC 1 cut(s) 2146
BlsI GCNGC 9 cut(s) 52, 170, 594, 731, 1384, 1476, 2144, 2671, 2894
BmcAI AGTACT 1 cut(s) 1629
Bme1390I CCNGG 8 cut(s) 209, 233, 278, 306, 1134, 1513, 2133, 3603
Bme18I GGWCC 2 cut(s) 145, 2953
BmgT120I GGNCC 5 cut(s) 145, 691, 2769, 2878, 2953
BmiI GGNNCC 5 cut(s) 537, 3072, 3324, 3552, 3600
BmrFI CCNGG 8 cut(s) 209, 233, 278, 306, 1134, 1513, 2133, 3603
BmsI GCATC 6 cut(s) 1171, 1839, 1993, 2524, 2598, 3625
BmtI GCTAGC 1 cut(s) 1511
BoxI GACNNNNGTC 1 cut(s) 2366
BpiI GAAGAC 3 cut(s) 1810, 3093, 3660
Bpu10I CCTNAGC 3 cut(s) 55, 1860, 2151
Bpu1102I GCTNAGC 1 cut(s) 2146
BpuEI CTTGAG 3 cut(s) 653, 2669, 3326
BpuMI CCSGG 5 cut(s) 209, 233, 278, 306, 1513
BsaAI YACGTR 2 cut(s) 2963, 3708
BsaI GGTCTC 2 cut(s) 142, 2284
BsaWI WCCGGW 4 cut(s) 142, 188, 2564, 3249
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bse118I RCCGGY 1 cut(s) 2564
Bse1I ACTGG 4 cut(s) 1541, 1587, 2418, 2427
Bse3DI GCAATG 1 cut(s) 1545
BseAI TCCGGA 2 cut(s) 142, 188
BseBI CCWGG 3 cut(s) 1134, 2133, 3603
BseGI GGATG 7 cut(s) 359, 568, 834, 1441, 1537, 2275, 3640
BseMI GCAATG 1 cut(s) 1545
BseNI ACTGG 4 cut(s) 1541, 1587, 2418, 2427
BseRI GAGGAG 1 cut(s) 1262
BseXI GCAGC 7 cut(s) 62, 155, 579, 716, 1369, 1486, 2129
BseYI CCCAGC 3 cut(s) 766, 1141, 2716
Bsh1236I CGCG 4 cut(s) 1270, 2451, 2484, 3177
BshFI GGCC 9 cut(s) 547, 693, 886, 1096, 1132, 2617, 2770, 2880, 3422
BshNI GGYRCC 1 cut(s) 535
BshTI ACCGGT 1 cut(s) 2564
BsiHKAI GWGCWC 4 cut(s) 161, 1873, 3225, 3448
BslFI GGGAC 6 cut(s) 961, 1090, 1163, 2374, 2880, 3347
BsmAI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BsmFI GGGAC 6 cut(s) 961, 1090, 1163, 2374, 2880, 3347
BsmI GAATGC 1 cut(s) 3392
BsnI GGCC 9 cut(s) 547, 693, 886, 1096, 1132, 2617, 2770, 2880, 3422
Bso31I GGTCTC 2 cut(s) 142, 2284
Bsp1286I GDGCHC 7 cut(s) 161, 958, 1873, 3059, 3225, 3448, 3669
Bsp13I TCCGGA 2 cut(s) 142, 188
Bsp1720I GCTNAGC 1 cut(s) 2146
Bsp68I TCGCGA 2 cut(s) 2451, 2484
BspACI CCGC 8 cut(s) 310, 663, 1775, 2021, 2670, 2893, 3177, 3463
BspANI GGCC 9 cut(s) 547, 693, 886, 1096, 1132, 2617, 2770, 2880, 3422
BspEI TCCGGA 2 cut(s) 142, 188
BspFNI CGCG 4 cut(s) 1270, 2451, 2484, 3177
BspHI TCATGA 3 cut(s) 735, 853, 3504
BspLI GGNNCC 5 cut(s) 537, 3072, 3324, 3552, 3600
BspMAI CTGCAG 1 cut(s) 711
BspOI GCTAGC 1 cut(s) 1511
BspPI GGATC 7 cut(s) 101, 173, 180, 3317, 3330, 3545, 3558
BspQI GCTCTTC 2 cut(s) 2544, 3657
BspT107I GGYRCC 1 cut(s) 535
BspTNI GGTCTC 2 cut(s) 142, 2284
BsrBI CCGCTC 1 cut(s) 2895
BsrDI GCAATG 1 cut(s) 1545
BsrFI RCCGGY 1 cut(s) 2564
BsrI ACTGG 4 cut(s) 1541, 1587, 2418, 2427
BssAI RCCGGY 1 cut(s) 2564
BssNAI GTATAC 1 cut(s) 3711
BssT1I CCWWGG 4 cut(s) 476, 1204, 2612, 3066
Bst1107I GTATAC 1 cut(s) 3711
Bst2UI CCWGG 3 cut(s) 1134, 2133, 3603
Bst6I CTCTTC 4 cut(s) 309, 462, 2544, 3657
BstAPI GCANNNNNTGC 3 cut(s) 1382, 3347, 3452
BstBAI YACGTR 2 cut(s) 2963, 3708
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 7 cut(s) 359, 568, 834, 1441, 1537, 2275, 3640
BstFNI CGCG 4 cut(s) 1270, 2451, 2484, 3177
BstH2I RGCGCY 2 cut(s) 2893, 3612
BstHHI GCGC 5 cut(s) 173, 1059, 1971, 2892, 3611
BstMAI GTCTC 5 cut(s) 142, 1314, 2039, 2284, 2336
BstNI CCWGG 3 cut(s) 1134, 2133, 3603
BstNSI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2732, 3350
BstPAI GACNNNNGTC 1 cut(s) 2366
BstSCI CCNGG 8 cut(s) 207, 231, 276, 304, 1132, 1511, 2131, 3601
BstSFI CTRYAG 1 cut(s) 707
BstSNI TACGTA 1 cut(s) 2963
BstUI CGCG 4 cut(s) 1270, 2451, 2484, 3177
BstV1I GCAGC 7 cut(s) 62, 155, 579, 716, 1369, 1486, 2129
BstV2I GAAGAC 3 cut(s) 1810, 3093, 3660
BstX2I RGATCY 5 cut(s) 185, 2207, 3143, 3322, 3550
BstYI RGATCY 5 cut(s) 185, 2207, 3143, 3322, 3550
BstZ17I GTATAC 1 cut(s) 3711
BsuI GTATCC 2 cut(s) 1918, 3137
BsuRI GGCC 9 cut(s) 547, 693, 886, 1096, 1132, 2617, 2770, 2880, 3422
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 7 cut(s) 359, 568, 834, 1441, 1537, 2275, 3640
BtsI GCAGTG 2 cut(s) 704, 2401
BtsIMutI CAGTG 4 cut(s) 388, 704, 2401, 2420
BtuMI TCGCGA 2 cut(s) 2451, 2484
CciI TCATGA 3 cut(s) 735, 853, 3504
CfoI GCGC 5 cut(s) 173, 1059, 1971, 2892, 3611
Cfr10I RCCGGY 1 cut(s) 2564
Cfr13I GGNCC 5 cut(s) 145, 691, 2769, 2878, 2953
CspAI ACCGGT 1 cut(s) 2564
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
DraI TTTAAA 3 cut(s) 298, 876, 1159
DraIII CACNNNGTG 1 cut(s) 3452
Eam1104I CTCTTC 4 cut(s) 309, 462, 2544, 3657
EarI CTCTTC 4 cut(s) 309, 462, 2544, 3657
EciI GGCGGA 1 cut(s) 678
Ecl136II GAGCTC 2 cut(s) 159, 1871
Eco105I TACGTA 1 cut(s) 2963
Eco130I CCWWGG 4 cut(s) 476, 1204, 2612, 3066
Eco24I GRGCYC 5 cut(s) 161, 958, 1873, 3059, 3669
Eco31I GGTCTC 2 cut(s) 142, 2284
Eco47I GGWCC 2 cut(s) 145, 2953
Eco53kI GAGCTC 2 cut(s) 159, 1871
Eco57I CTGAAG 4 cut(s) 1407, 1838, 2529, 2894
EcoICRI GAGCTC 2 cut(s) 159, 1871
EcoRI GAATTC 2 cut(s) 1357, 2741
EcoRII CCWGG 3 cut(s) 1132, 2131, 3601
EcoT14I CCWWGG 4 cut(s) 476, 1204, 2612, 3066
EcoT22I ATGCAT 1 cut(s) 3343
EcoT38I GRGCYC 5 cut(s) 161, 958, 1873, 3059, 3669
ErhI CCWWGG 4 cut(s) 476, 1204, 2612, 3066
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 6 cut(s) 961, 1090, 1163, 2374, 2880, 3347
FauI CCCGC 2 cut(s) 303, 1768
FbaI TGATCA 1 cut(s) 2433
FblI GTMKAC 2 cut(s) 498, 3710
Fnu4HI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2670, 2893
FokI GGATG 7 cut(s) 346, 575, 821, 1448, 1524, 2282, 3647
FriOI GRGCYC 5 cut(s) 161, 958, 1873, 3059, 3669
Fsp4HI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2670, 2893
GlaI GCGC 5 cut(s) 172, 1058, 1970, 2891, 3610
GluI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2670, 2893
GsaI CCCAGC 3 cut(s) 770, 1145, 2720
HaeII RGCGCY 2 cut(s) 2893, 3612
HaeIII GGCC 9 cut(s) 547, 693, 886, 1096, 1132, 2617, 2770, 2880, 3422
HhaI GCGC 5 cut(s) 173, 1059, 1971, 2892, 3611
Hin6I GCGC 5 cut(s) 171, 1057, 1969, 2890, 3609
HinP1I GCGC 5 cut(s) 171, 1057, 1969, 2890, 3609
HincII GTYRAC 3 cut(s) 205, 1900, 3468
HindII GTYRAC 3 cut(s) 205, 1900, 3468
HpaI GTTAAC 1 cut(s) 205
HphI GGTGA 8 cut(s) 911, 1475, 1610, 1830, 2072, 2563, 3347, 3395
Hpy166II GTNNAC 8 cut(s) 205, 499, 1900, 2956, 3032, 3247, 3468, 3711
Hpy8I GTNNAC 8 cut(s) 205, 499, 1900, 2956, 3032, 3247, 3468, 3711
HpyAV CCTTC 9 cut(s) 737, 1092, 1140, 1288, 1915, 2351, 2507, 2837, 2869
HpyCH4IV ACGT 3 cut(s) 1288, 2962, 3707
HpySE526I ACGT 3 cut(s) 1288, 2962, 3707
HspAI GCGC 5 cut(s) 171, 1057, 1969, 2890, 3609
Kpn2I TCCGGA 2 cut(s) 142, 188
KpnI GGTACC 1 cut(s) 539
Ksp22I TGATCA 1 cut(s) 2433
KspAI GTTAAC 1 cut(s) 205
LguI GCTCTTC 2 cut(s) 2544, 3657
LmnI GCTCC 5 cut(s) 444, 1825, 3220, 3474, 3606
Lsp1109I GCAGC 7 cut(s) 62, 155, 579, 716, 1369, 1486, 2129
LweI GCATC 6 cut(s) 1171, 1839, 1993, 2524, 2598, 3625
MaeII ACGT 3 cut(s) 1288, 2962, 3707
MbiI CCGCTC 1 cut(s) 2895
MfeI CAATTG 1 cut(s) 2499
MflI RGATCY 5 cut(s) 185, 2207, 3143, 3322, 3550
MhlI GDGCHC 7 cut(s) 161, 958, 1873, 3059, 3225, 3448, 3669
MlyI GAGTC 9 cut(s) 254, 983, 1411, 1586, 1946, 2180, 2563, 2660, 3403
MmeI TCCRAC 6 cut(s) 540, 1560, 1756, 2304, 3180, 3350
Mph1103I ATGCAT 1 cut(s) 3343
MroI TCCGGA 2 cut(s) 142, 188
MroXI GAANNNNTTC 4 cut(s) 2457, 2490, 2692, 3162
MslI CAYNNNNRTG 3 cut(s) 384, 1434, 3432
MspA1I CMGCKG 2 cut(s) 1775, 2672
MspR9I CCNGG 8 cut(s) 209, 233, 278, 306, 1134, 1513, 2133, 3603
MunI CAATTG 1 cut(s) 2499
Mva1269I GAATGC 1 cut(s) 3392
MvaI CCWGG 3 cut(s) 1134, 2133, 3603
MvnI CGCG 4 cut(s) 1270, 2451, 2484, 3177
NciI CCSGG 5 cut(s) 209, 233, 278, 306, 1513
NheI GCTAGC 1 cut(s) 1507
NlaIV GGNNCC 5 cut(s) 537, 3072, 3324, 3552, 3600
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 6 cut(s) 1403, 1836, 1938, 2652, 3152, 3509
NruI TCGCGA 2 cut(s) 2451, 2484
NsiI ATGCAT 1 cut(s) 3343
NspI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2732, 3350
OliI CACNNNNGTG 1 cut(s) 384
PaeI GCATGC 4 cut(s) 729, 940, 2732, 3350
PagI TCATGA 3 cut(s) 735, 853, 3504
PciI ACATGT 4 cut(s) 1257, 1300, 1810, 2066
PciSI GCTCTTC 2 cut(s) 2544, 3657
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PctI GAATGC 1 cut(s) 3392
PdmI GAANNNNTTC 4 cut(s) 2457, 2490, 2692, 3162
PfeI GAWTC 7 cut(s) 80, 611, 1583, 2417, 2571, 2603, 3685
PflFI GACNNNGTC 1 cut(s) 669
PinAI ACCGGT 1 cut(s) 2564
PkrI GCNGC 9 cut(s) 52, 170, 594, 731, 1384, 1476, 2144, 2671, 2894
PleI GAGTC 9 cut(s) 254, 982, 1410, 1585, 1945, 2179, 2562, 2659, 3403
PpsI GAGTC 9 cut(s) 254, 982, 1410, 1585, 1945, 2179, 2562, 2659, 3403
Ppu21I YACGTR 2 cut(s) 2963, 3708
PscI ACATGT 4 cut(s) 1257, 1300, 1810, 2066
PshAI GACNNNNGTC 1 cut(s) 2366
PshBI ATTAAT 1 cut(s) 2789
PsiI TTATAA 1 cut(s) 405
Psp124BI GAGCTC 2 cut(s) 161, 1873
Psp6I CCWGG 3 cut(s) 1132, 2131, 3601
PspFI CCCAGC 3 cut(s) 766, 1141, 2716
PspGI CCWGG 3 cut(s) 1132, 2131, 3601
PspN4I GGNNCC 5 cut(s) 537, 3072, 3324, 3552, 3600
PspPI GGNCC 5 cut(s) 145, 691, 2769, 2878, 2953
PstI CTGCAG 1 cut(s) 711
PsuI RGATCY 5 cut(s) 185, 2207, 3143, 3322, 3550
PsyI GACNNNGTC 1 cut(s) 669
RruI TCGCGA 2 cut(s) 2451, 2484
RseI CAYNNNNRTG 3 cut(s) 384, 1434, 3432
SacI GAGCTC 2 cut(s) 161, 1873
SapI GCTCTTC 2 cut(s) 2544, 3657
SatI GCNGC 9 cut(s) 51, 169, 593, 730, 1383, 1475, 2143, 2670, 2893
Sau96I GGNCC 5 cut(s) 145, 691, 2769, 2878, 2953
ScaI AGTACT 1 cut(s) 1629
SchI GAGTC 9 cut(s) 254, 983, 1411, 1586, 1946, 2180, 2563, 2660, 3403
ScrFI CCNGG 8 cut(s) 209, 233, 278, 306, 1134, 1513, 2133, 3603
SduI GDGCHC 7 cut(s) 161, 958, 1873, 3059, 3225, 3448, 3669
SfaNI GCATC 6 cut(s) 1171, 1839, 1993, 2524, 2598, 3625
SfcI CTRYAG 1 cut(s) 707
SinI GGWCC 2 cut(s) 145, 2953
SmiMI CAYNNNNRTG 3 cut(s) 384, 1434, 3432
SmlI CTYRAG 3 cut(s) 632, 2648, 3305
SmoI CTYRAG 3 cut(s) 632, 2648, 3305
SnaBI TACGTA 1 cut(s) 2963
SpeI ACTAGT 1 cut(s) 3134
SphI GCATGC 4 cut(s) 729, 940, 2732, 3350
SsiI CCGC 8 cut(s) 310, 663, 1775, 2021, 2670, 2893, 3177, 3463
SspI AATATT 2 cut(s) 1608, 3080
SstI GAGCTC 2 cut(s) 161, 1873
StyD4I CCNGG 8 cut(s) 207, 231, 276, 304, 1132, 1511, 2131, 3601
StyI CCWWGG 4 cut(s) 476, 1204, 2612, 3066
TaiI ACGT 3 cut(s) 1291, 2965, 3710
TaqI TCGA 3 cut(s) 243, 946, 2542
TatI WGTACW 4 cut(s) 417, 1348, 1627, 1639
TauI GCSGC 2 cut(s) 2672, 2895
TfiI GAWTC 7 cut(s) 80, 611, 1583, 2417, 2571, 2603, 3685
TscAI CASTG 4 cut(s) 388, 711, 2401, 2427
TseFI GTSAC 6 cut(s) 1403, 1836, 1938, 2652, 3152, 3509
TseI GCWGC 7 cut(s) 50, 168, 592, 729, 1382, 1474, 2142
Tsp45I GTSAC 6 cut(s) 1403, 1836, 1938, 2652, 3152, 3509
TspGWI ACGGA 2 cut(s) 99, 1650
TspRI CASTG 4 cut(s) 388, 711, 2401, 2427
Tth111I GACNNNGTC 1 cut(s) 669
VpaK11BI GGWCC 2 cut(s) 145, 2953
VspI ATTAAT 1 cut(s) 2789
XceI RCATGY 8 cut(s) 729, 940, 1261, 1304, 1814, 2070, 2732, 3350
XcmI CCANNNNNNNNNTGG 2 cut(s) 845, 2963
XmiI GTMKAC 2 cut(s) 498, 3710
XmnI GAANNNNTTC 4 cut(s) 2457, 2490, 2692, 3162
ZrmI AGTACT 1 cut(s) 1629
Zsp2I ATGCAT 1 cut(s) 3343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.