Rmu_sc0000003.1_g000001

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000003.1
OG0006789
Physical Location & Seq
Reverse (-)
2 .. 6836
6835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000003.1_g000001.1.cds

Sequence Viewer

Length: 2222 bp
atggaaatcaccgaagaacagcgaaagcgagccgaggcgaatcgattggctgccttagcaaaacgcaaagccctcgccgaaacctccaatcttcaacaacaacacgatccgtggtcgcttttcaaatgccaaaaggtctctccggacctcaatccgagctcaattcgctgcgcccgcgatccaagatccggaatttcacgggttagcccggttgtggagaaccagatgttccgggtcaggctcgaaatctgctcgccggactcgttctcagccaccccggttgccgttcaagggtttaaatttccggggcgggaagagtgcgtaaggagaatgagtgattgcttggctggtgtaatgccatcccactacacgcaaaatcacagtggtggaaaggctggtgtttataagctgagtgagtacaaagaagtcttgaaatgtttaaggagcaacaagggcattgacattgaagagataccttggactactttccatgttgtagacagactttcacagtcatatatcacagggaagtgggtaccatgcaggccagaacacttgttggatgataaggttgatgagttgatcgggaagctgccaaagaggctattggattcactcttacctttccagcttgagggtgttagatttgctattttgcgttattcatgggcagaagaattagagcattggcttcctttttgtttgcctgctgacatccatctagtttttggtcatgagaacaatcctgctaatttaaagaaatggcctagagttgtggttatttcttatacaatgcttcaccatttgcaaaagagcatgctagatcgagaatgggctctcttgattgttgatgagtcccaccatgtacgatgtacgaagaaaaaatcagaacctagagagataaaagctgttcttgatgtggcaagaaaggtcaagcgcatagtgttactatcggggacaccttctttgtcaaggccatttgacatctttcatcagatagatatgttatggcctggtttgctgggaagggacaagtttaaatttggagaaacttactgtgatgccaaatatgttcggggtgtccaagggaaagtttttcaggatttctcaaagggtactcgtttggaggagttgaacatgttactaacgcgaacagttatgataagacgtttgaaggaacatgtcctgtctcagctaccacccaaacgccgacagattatacaagttgtactaaagaaatcagatatagtttcagcaaaggctgctatcagggtgggaaagagtcacgatgaagatgtatcttcagaacatttggatgaactgaatgatagtatgggttgctgtatatcaaagcagctttctcaccaagaattaggtattgcaaagctagccgggtttcgtgaatggctctccattcatccagtcattgcagaggcagatggggttgcaaaattggagtcggattccagttctcataaaatgttaatatttgcacatcaccataaagtacttgacggagtacaggagttcataatccacaaagagatagattttgtccgaattgatggaaacacacttgccacagacaggcagttagctgtgcgaaaattccaactatcaagtgaggttaagattgcaataattggtattacagcgggaggtgttggtcttgatttctcatcagcaacacatgttgtcttcttggagcttcctcagtcaccatcattgatgcttcaggctgaggatagagctcacaggcgagggcaaacaaatgcagtcaacatatacttcttttctgggaaggataccattgatgagtcacattggcaatacttgaacaggagtttgcgccgtgtttcatctacaacaaatggaaaatatgatgcaatacaagaaatagcggttgaagatgtttctttttttgagacattgggtggaggtgatacatgtgaagattatactttgcaaaagtcagagggtagtgagttctcagcagagctaatcaaagttcctggttctggctgcttagctaaggctatgaagccttttgagtcaaatgataaattagtgccaaacattccacagagatctgaaagacatcatggtactgatggcatttcatcacaaactgaaaattctgttattaaggatgatgtggtctctgatttggatatggataacagtatctctttagatgagaagttggaaacaaatgttcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

740

Amino Acids

83.64

Weight (kDa)

7.16

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 535
AccI GTMKAC 1 cut(s) 498
AccII CGCG 2 cut(s) 177, 1150
AccIII TCCGGA 2 cut(s) 142, 188
AciI CCGC 4 cut(s) 175, 310, 1655, 1901
AclWI GGATC 3 cut(s) 101, 173, 180
AcsI RAATTY 5 cut(s) 192, 299, 1040, 1607, 2134
AcuI CTGAAG 2 cut(s) 1287, 1718
AfaI GTAC 9 cut(s) 419, 537, 867, 874, 1117, 1230, 1509, 1521, 2107
AfiI CCNNNNNNNGG 6 cut(s) 188, 214, 291, 634, 1587, 2018
AflIII ACRYGT 4 cut(s) 1137, 1180, 1690, 1946
AgsI TTSAA 9 cut(s) 95, 124, 290, 433, 467, 1135, 1174, 1837, 1907
AjnI CCWGG 2 cut(s) 1012, 2011
AleI CACNNNNGTG 1 cut(s) 384
Alw21I GWGCWC 2 cut(s) 161, 1753
Alw26I GTCTC 4 cut(s) 142, 1194, 1919, 2164
AlwI GGATC 3 cut(s) 101, 173, 180
Aor13HI TCCGGA 2 cut(s) 142, 188
AoxI GGCC 4 cut(s) 545, 764, 974, 1010
ApeKI GCWGC 6 cut(s) 50, 168, 592, 1262, 1354, 2022
ApoI RAATTY 5 cut(s) 192, 299, 1040, 1607, 2134
ArsI GACNNNNNNTTYG 4 cut(s) 949, 981, 1442, 1474
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 3 cut(s) 173, 939, 1851
AspS9I GGNCC 1 cut(s) 145
AsuC2I CCSGG 5 cut(s) 209, 233, 278, 306, 1393
AsuHPI GGTGA 5 cut(s) 791, 1355, 1490, 1710, 1952
AsuNHI GCTAGC 1 cut(s) 1387
AvaII GGWCC 1 cut(s) 145
BanI GGYRCC 1 cut(s) 535
BanII GRGCYC 3 cut(s) 161, 838, 1753
BbsI GAAGAC 1 cut(s) 1690
Bbv12I GWGCWC 2 cut(s) 161, 1753
BbvCI CCTCAGC 1 cut(s) 1740
BbvI GCAGC 6 cut(s) 37, 155, 579, 1249, 1366, 2009
BccI CCATC 6 cut(s) 367, 726, 1433, 1559, 1729, 2105
BceAI ACGGC 2 cut(s) 269, 1836
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 2 cut(s) 1014, 2013
BciVI GTATCC 1 cut(s) 1798
BcnI CCSGG 5 cut(s) 209, 233, 278, 306, 1393
BcoDI GTCTC 4 cut(s) 142, 1194, 1919, 2164
BfaI CTAG 5 cut(s) 722, 768, 821, 894, 1388
BfuI GTATCC 1 cut(s) 1798
BglI GCCNNNNNGGC 1 cut(s) 601
BglII AGATCT 1 cut(s) 2087
BisI GCNGC 6 cut(s) 51, 169, 593, 1263, 1355, 2023
BlpI GCTNAGC 1 cut(s) 2026
BlsI GCNGC 6 cut(s) 52, 170, 594, 1264, 1356, 2024
BmcAI AGTACT 1 cut(s) 1509
Bme1390I CCNGG 7 cut(s) 209, 233, 278, 306, 1014, 1393, 2013
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 7 cut(s) 209, 233, 278, 306, 1014, 1393, 2013
BmsI GCATC 3 cut(s) 1051, 1719, 1873
BmtI GCTAGC 1 cut(s) 1391
BpiI GAAGAC 1 cut(s) 1690
Bpu10I CCTNAGC 3 cut(s) 55, 1740, 2031
Bpu1102I GCTNAGC 1 cut(s) 2026
BpuEI CTTGAG 1 cut(s) 653
BpuMI CCSGG 5 cut(s) 209, 233, 278, 306, 1393
Bsa29I ATCGAT 1 cut(s) 43
BsaI GGTCTC 2 cut(s) 142, 2164
BsaJI CCNNGG 6 cut(s) 33, 110, 276, 305, 476, 1084
BsaWI WCCGGW 2 cut(s) 142, 188
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 6 cut(s) 188, 214, 291, 634, 1587, 2018
Bse1I ACTGG 2 cut(s) 1421, 1467
Bse3DI GCAATG 1 cut(s) 1425
BseAI TCCGGA 2 cut(s) 142, 188
BseBI CCWGG 2 cut(s) 1014, 2013
BseCI ATCGAT 1 cut(s) 43
BseDI CCNNGG 6 cut(s) 33, 110, 276, 305, 476, 1084
BseGI GGATG 6 cut(s) 359, 568, 714, 1321, 1417, 2155
BseLI CCNNNNNNNGG 6 cut(s) 188, 214, 291, 634, 1587, 2018
BseMI GCAATG 1 cut(s) 1425
BseMII CTCAG 6 cut(s) 282, 401, 1205, 1727, 1731, 2004
BseNI ACTGG 2 cut(s) 1421, 1467
BseRI GAGGAG 1 cut(s) 1142
BseXI GCAGC 6 cut(s) 37, 155, 579, 1249, 1366, 2009
BseYI CCCAGC 1 cut(s) 1021
Bsh1236I CGCG 2 cut(s) 177, 1150
BshFI GGCC 4 cut(s) 547, 766, 976, 1012
BshNI GGYRCC 1 cut(s) 535
BshVI ATCGAT 1 cut(s) 43
BsiHKAI GWGCWC 2 cut(s) 161, 1753
BsiSI CCGG 8 cut(s) 143, 189, 209, 232, 257, 278, 305, 1392
BslFI GGGAC 3 cut(s) 841, 970, 1043
BslI CCNNNNNNNGG 6 cut(s) 188, 214, 291, 634, 1587, 2018
BsmAI GTCTC 4 cut(s) 142, 1194, 1919, 2164
BsmFI GGGAC 3 cut(s) 841, 970, 1043
BsnI GGCC 4 cut(s) 547, 766, 976, 1012
Bso31I GGTCTC 2 cut(s) 142, 2164
Bsp1286I GDGCHC 3 cut(s) 161, 838, 1753
Bsp13I TCCGGA 2 cut(s) 142, 188
Bsp143I GATC 6 cut(s) 106, 178, 185, 582, 823, 2087
Bsp1720I GCTNAGC 1 cut(s) 2026
BspACI CCGC 4 cut(s) 175, 310, 1655, 1901
BspANI GGCC 4 cut(s) 547, 766, 976, 1012
BspCNI CTCAG 6 cut(s) 281, 402, 1204, 1726, 1732, 2003
BspDI ATCGAT 1 cut(s) 43
BspEI TCCGGA 2 cut(s) 142, 188
BspFNI CGCG 2 cut(s) 177, 1150
BspHI TCATGA 1 cut(s) 733
BspLI GGNNCC 1 cut(s) 537
BspOI GCTAGC 1 cut(s) 1391
BspPI GGATC 3 cut(s) 101, 173, 180
BspT107I GGYRCC 1 cut(s) 535
BspTNI GGTCTC 2 cut(s) 142, 2164
BsrDI GCAATG 1 cut(s) 1425
BsrI ACTGG 2 cut(s) 1421, 1467
BssECI CCNNGG 6 cut(s) 33, 110, 276, 305, 476, 1084
BssMI GATC 6 cut(s) 106, 178, 185, 582, 823, 2087
BssT1I CCWWGG 2 cut(s) 476, 1084
Bst2UI CCWGG 2 cut(s) 1014, 2013
Bst4CI ACNGT 5 cut(s) 383, 513, 1058, 1156, 2183
Bst6I CTCTTC 2 cut(s) 309, 462
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstC8I GCNNGC 7 cut(s) 30, 175, 254, 545, 708, 818, 1389
BstDEI CTNAG 9 cut(s) 55, 268, 410, 1191, 1713, 1740, 1990, 2026, 2031
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 6 cut(s) 359, 568, 714, 1321, 1417, 2155
BstFNI CGCG 2 cut(s) 177, 1150
BstHHI GCGC 3 cut(s) 173, 939, 1851
BstKTI GATC 6 cut(s) 109, 181, 188, 585, 826, 2090
BstMAI GTCTC 4 cut(s) 142, 1194, 1919, 2164
BstMBI GATC 6 cut(s) 106, 178, 185, 582, 823, 2087
BstMWI GCNNNNNNNGC 8 cut(s) 56, 165, 174, 453, 601, 1018, 1262, 1388
BstNI CCWGG 2 cut(s) 1014, 2013
BstNSI RCATGY 5 cut(s) 820, 1141, 1184, 1694, 1950
BstSCI CCNGG 7 cut(s) 207, 231, 276, 304, 1012, 1391, 2011
BstUI CGCG 2 cut(s) 177, 1150
BstV1I GCAGC 6 cut(s) 37, 155, 579, 1249, 1366, 2009
BstV2I GAAGAC 1 cut(s) 1690
BstX2I RGATCY 2 cut(s) 185, 2087
BstYI RGATCY 2 cut(s) 185, 2087
Bsu15I ATCGAT 1 cut(s) 43
BsuI GTATCC 1 cut(s) 1798
BsuRI GGCC 4 cut(s) 547, 766, 976, 1012
BsuTUI ATCGAT 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 110
BtsCI GGATG 6 cut(s) 359, 568, 714, 1321, 1417, 2155
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 7 cut(s) 30, 175, 254, 545, 708, 818, 1389
CciI TCATGA 1 cut(s) 733
CfoI GCGC 3 cut(s) 173, 939, 1851
Cfr13I GGNCC 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 43
Csp6I GTAC 9 cut(s) 418, 536, 866, 873, 1116, 1229, 1508, 1520, 2106
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviQI GTAC 9 cut(s) 418, 536, 866, 873, 1116, 1229, 1508, 1520, 2106
DdeI CTNAG 9 cut(s) 55, 268, 410, 1191, 1713, 1740, 1990, 2026, 2031
DpnI GATC 6 cut(s) 108, 180, 187, 584, 825, 2089
DpnII GATC 6 cut(s) 106, 178, 185, 582, 823, 2087
DraI TTTAAA 3 cut(s) 298, 756, 1039
Eam1104I CTCTTC 2 cut(s) 309, 462
EarI CTCTTC 2 cut(s) 309, 462
Ecl136II GAGCTC 2 cut(s) 159, 1751
Eco130I CCWWGG 2 cut(s) 476, 1084
Eco24I GRGCYC 3 cut(s) 161, 838, 1753
Eco31I GGTCTC 2 cut(s) 142, 2164
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 2 cut(s) 159, 1751
Eco57I CTGAAG 2 cut(s) 1287, 1718
EcoICRI GAGCTC 2 cut(s) 159, 1751
EcoRII CCWGG 2 cut(s) 1012, 2011
EcoT14I CCWWGG 2 cut(s) 476, 1084
EcoT38I GRGCYC 3 cut(s) 161, 838, 1753
ErhI CCWWGG 2 cut(s) 476, 1084
FalI AAGNNNNNCTT 2 cut(s) 897, 929
FaqI GGGAC 3 cut(s) 841, 970, 1043
FauI CCCGC 3 cut(s) 182, 303, 1648
FblI GTMKAC 1 cut(s) 498
Fnu4HI GCNGC 6 cut(s) 51, 169, 593, 1263, 1355, 2023
FokI GGATG 6 cut(s) 346, 575, 701, 1328, 1404, 2162
FriOI GRGCYC 3 cut(s) 161, 838, 1753
Fsp4HI GCNGC 6 cut(s) 51, 169, 593, 1263, 1355, 2023
FspBI CTAG 5 cut(s) 722, 768, 821, 894, 1388
GlaI GCGC 3 cut(s) 172, 938, 1850
GluI GCNGC 6 cut(s) 51, 169, 593, 1263, 1355, 2023
GsaI CCCAGC 1 cut(s) 1025
HaeIII GGCC 4 cut(s) 547, 766, 976, 1012
HapII CCGG 8 cut(s) 143, 189, 209, 232, 257, 278, 305, 1392
HhaI GCGC 3 cut(s) 173, 939, 1851
Hin6I GCGC 3 cut(s) 171, 937, 1849
HinP1I GCGC 3 cut(s) 171, 937, 1849
HincII GTYRAC 1 cut(s) 1780
HindII GTYRAC 1 cut(s) 1780
HinfI GANTC 9 cut(s) 40, 260, 611, 854, 1282, 1457, 1463, 1817, 2051
HpaII CCGG 8 cut(s) 143, 189, 209, 232, 257, 278, 305, 1392
HphI GGTGA 5 cut(s) 791, 1355, 1490, 1710, 1952
Hpy166II GTNNAC 2 cut(s) 499, 1780
Hpy8I GTNNAC 2 cut(s) 499, 1780
HpyAV CCTTC 4 cut(s) 972, 1020, 1168, 1795
HpyCH4III ACNGT 5 cut(s) 383, 513, 1058, 1156, 2183
HpyCH4IV ACGT 1 cut(s) 1168
HpyF10VI GCNNNNNNNGC 8 cut(s) 56, 165, 174, 453, 601, 1018, 1262, 1388
HpyF3I CTNAG 9 cut(s) 55, 268, 410, 1191, 1713, 1740, 1990, 2026, 2031
HpySE526I ACGT 1 cut(s) 1168
HspAI GCGC 3 cut(s) 171, 937, 1849
Kpn2I TCCGGA 2 cut(s) 142, 188
KpnI GGTACC 1 cut(s) 539
Kzo9I GATC 6 cut(s) 106, 178, 185, 582, 823, 2087
LmnI GCTCC 2 cut(s) 444, 1705
Lsp1109I GCAGC 6 cut(s) 37, 155, 579, 1249, 1366, 2009
LweI GCATC 3 cut(s) 1051, 1719, 1873
MaeI CTAG 5 cut(s) 722, 768, 821, 894, 1388
MaeII ACGT 1 cut(s) 1168
MaeIII GTNAC 5 cut(s) 945, 1140, 1283, 1716, 1818
MalI GATC 6 cut(s) 108, 180, 187, 584, 825, 2089
MboI GATC 6 cut(s) 106, 178, 185, 582, 823, 2087
MflI RGATCY 2 cut(s) 185, 2087
MhlI GDGCHC 3 cut(s) 161, 838, 1753
MlyI GAGTC 6 cut(s) 254, 863, 1291, 1466, 1826, 2060
MmeI TCCRAC 4 cut(s) 540, 1440, 1636, 2184
MroI TCCGGA 2 cut(s) 142, 188
MseI TTAA 7 cut(s) 297, 440, 755, 1038, 1484, 1629, 2145
MslI CAYNNNNRTG 2 cut(s) 384, 1314
MspA1I CMGCKG 1 cut(s) 1655
MspI CCGG 8 cut(s) 143, 189, 209, 232, 257, 278, 305, 1392
MspR9I CCNGG 7 cut(s) 209, 233, 278, 306, 1014, 1393, 2013
MvaI CCWGG 2 cut(s) 1014, 2013
MvnI CGCG 2 cut(s) 177, 1150
MwoI GCNNNNNNNGC 8 cut(s) 56, 165, 174, 453, 601, 1018, 1262, 1388
NciI CCSGG 5 cut(s) 209, 233, 278, 306, 1393
NdeII GATC 6 cut(s) 106, 178, 185, 582, 823, 2087
NheI GCTAGC 1 cut(s) 1387
NlaIV GGNNCC 1 cut(s) 537
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 3 cut(s) 1283, 1716, 1818
NspI RCATGY 5 cut(s) 820, 1141, 1184, 1694, 1950
OliI CACNNNNGTG 1 cut(s) 384
PaeI GCATGC 1 cut(s) 820
PagI TCATGA 1 cut(s) 733
PciI ACATGT 4 cut(s) 1137, 1180, 1690, 1946
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PfeI GAWTC 3 cut(s) 40, 611, 1463
PkrI GCNGC 6 cut(s) 52, 170, 594, 1264, 1356, 2024
PleI GAGTC 6 cut(s) 254, 862, 1290, 1465, 1825, 2059
PpsI GAGTC 6 cut(s) 254, 862, 1290, 1465, 1825, 2059
PscI ACATGT 4 cut(s) 1137, 1180, 1690, 1946
PsiI TTATAA 1 cut(s) 405
Psp124BI GAGCTC 2 cut(s) 161, 1753
Psp6I CCWGG 2 cut(s) 1012, 2011
PspFI CCCAGC 1 cut(s) 1021
PspGI CCWGG 2 cut(s) 1012, 2011
PspN4I GGNNCC 1 cut(s) 537
PspPI GGNCC 1 cut(s) 145
PsuI RGATCY 2 cut(s) 185, 2087
RsaI GTAC 9 cut(s) 419, 537, 867, 874, 1117, 1230, 1509, 1521, 2107
RsaNI GTAC 9 cut(s) 418, 536, 866, 873, 1116, 1229, 1508, 1520, 2106
RseI CAYNNNNRTG 2 cut(s) 384, 1314
SacI GAGCTC 2 cut(s) 161, 1753
SaqAI TTAA 7 cut(s) 297, 440, 755, 1038, 1484, 1629, 2145
SatI GCNGC 6 cut(s) 51, 169, 593, 1263, 1355, 2023
Sau3AI GATC 6 cut(s) 106, 178, 185, 582, 823, 2087
Sau96I GGNCC 1 cut(s) 145
ScaI AGTACT 1 cut(s) 1509
SchI GAGTC 6 cut(s) 254, 863, 1291, 1466, 1826, 2060
ScrFI CCNGG 7 cut(s) 209, 233, 278, 306, 1014, 1393, 2013
SduI GDGCHC 3 cut(s) 161, 838, 1753
SfaNI GCATC 3 cut(s) 1051, 1719, 1873
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 2 cut(s) 384, 1314
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
SphI GCATGC 1 cut(s) 820
SsiI CCGC 4 cut(s) 175, 310, 1655, 1901
SspI AATATT 1 cut(s) 1488
SspMI CTAG 5 cut(s) 722, 768, 821, 894, 1388
SstI GAGCTC 2 cut(s) 161, 1753
StyD4I CCNGG 7 cut(s) 207, 231, 276, 304, 1012, 1391, 2011
StyI CCWWGG 2 cut(s) 476, 1084
TaaI ACNGT 5 cut(s) 383, 513, 1058, 1156, 2183
TaiI ACGT 1 cut(s) 1171
TaqI TCGA 3 cut(s) 43, 243, 826
TatI WGTACW 4 cut(s) 417, 1228, 1507, 1519
TfiI GAWTC 3 cut(s) 40, 611, 1463
Tru1I TTAA 7 cut(s) 297, 440, 755, 1038, 1484, 1629, 2145
Tru9I TTAA 7 cut(s) 297, 440, 755, 1038, 1484, 1629, 2145
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 3 cut(s) 1283, 1716, 1818
TseI GCWGC 6 cut(s) 50, 168, 592, 1262, 1354, 2022
Tsp45I GTSAC 3 cut(s) 1283, 1716, 1818
TspDTI ATGAA 9 cut(s) 654, 980, 1305, 1332, 1406, 1519, 1848, 2054, 2109
TspGWI ACGGA 2 cut(s) 99, 1530
TspRI CASTG 1 cut(s) 388
VpaK11BI GGWCC 1 cut(s) 145
XapI RAATTY 5 cut(s) 192, 299, 1040, 1607, 2134
XceI RCATGY 5 cut(s) 820, 1141, 1184, 1694, 1950
XcmI CCANNNNNNNNNTGG 1 cut(s) 725
XmiI GTMKAC 1 cut(s) 498
XspI CTAG 5 cut(s) 722, 768, 821, 894, 1388
ZrmI AGTACT 1 cut(s) 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.