Rh7CG051900

DNA annealing helicase and endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006789
Physical Location & Seq
Forward (+)
3781119 .. 3788601
7483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG051900.1

Sequence Viewer

Length: 2634 bp
ATGTTATGGCCTGGTTTGCTGGGAAGGGACAAGTTTAAATTTGGAGAAACTTACTGTGATGCCAAATATGTTCGGGGTGTCCAAGGGAAAGTTTTTCAGGATTTCTCAAAGGGTACTCGTTTGGAGGAGTTGAACATGTTACTAACGCGAACAGTTATGATAAGACGTTTGAAGGAACATGTCCTGTCTCAGCTACCACCCAAACGCCGACAGATTATACAAGTTGTACTAAAGAAATCAGATATAGTTTCAGCAAAGGCTGCTATCAGGGTGGGAAAGAGTCACGATGAAGATGTATCTTCAGAACATTTGGATGAACTGAATGATAGTATGGGTTGCTGCATATCAAAGCAGCTTTCTCACCAAGAATTAGGTATTGCAAAGCTAGCTGGGTTTCGTGAATGGCTCTCCATTCATCCAGTCATTGCAGAGGCAGATGGGGTTGCAAAATTGGAGTCGGATTCCAGTTCTCATAAAATGTTAATATTTGCACATCACCATAAAGTACTTGACGGAGTACAGGAGTTCATAATCCACAAAGAGATAGATTTTGTCCGAATTGATGGAAACACACTTGCCACAGATAGGCAGTTAGCTGTGCGAAAATTCCAACTATCAACTGAGGTTAAGATTGCAATAGTTGGTATTACAGCAGGAGGTGTTGGTCTTGATTTTTCATCAGCAACACATGTTGTCTTCTTGGAGCTTCCTCAGTCACCATCATTGATGCTTCAGGCTGAGGATAGAGCTCACAGGCGAGGGCAAACAAATGCAGTCAACATATACTTCTTTTCTGGGAAGGATACCATTGATGAGTCACATTGGCAATACTTGAACAGGAGTTTGCGCCGTGTTTCATCTACAACAAATGGAAAATATGATGCAATACAAGAAATAGCGGTTGAAGATGTTTCTTTTTTTGAGACATTAGGTGGAGGTGATACATGTGAAGATTATGCTTTGCAAAAGTCAGAGGGTAGTGAGTTCTCAGCAGAGCTAATCAAAGTTCCTGGTTCTGGCTGCTTGGCTAAGGCTATGAAGCCTTTTGAGTCAAATGATAAGTTAGTGCCAAACATTCCACAGAGATCTGAAAGACATCATGGTACTGATGGCATTTCATCACAAACTGAAAATTGTGTTATTAAGGATGATGTGGTCTCTGATTTGGATATGGATAACAGTATCTCTTTAGATGAGAAGTTGGAAACAAATGTTCCTGAGACTAAAATTAGAGAAGGGACAGTAGTCTATTCTTGTAAGTTAAATAAGAGCAGTGAAGATAGAGACGGACTGGAATCACTGGATAAGAAGCAGGAAGACGGAATCAAATCTCAGACAAACAAAGGACATGATGGCCAATCTGTTCAGCCGATCAAAGGACATGATGGCGAACCTGTTCAGCCAATTGAAGCTGAAGAAGGTGGCACAAACCAAGTAGATGCTCTTCGATTTGAGGTGAGTCAATATACGGGTAGAATCCACTTGTATTCTTGCATCTCAGGAGAGGATTCAAGACCAAGGCCACTTTTTGAGAATTTTAGACCAGAGGAACTTGAGTCACTAAGTTCTTCTGCCGCTGAAAGTATTAAAGGAACAGCTTCAAATTCATTCAAGGATAACCCAGCTTATTTGCATGCACTTCTTGAATTCTATAAAGAATGGAAGAAACTGAGGCCCATTGAACAAAAGAAATTAATTGGAAAGCCTTTGCAACTTCCTTTGACTGTTGAGTTGTGCTTTCTGTGTGAAGGCACTAACCATGACAATACGGGACTGCTGAAGGGCCGAAGCAAGCGCCGCTCTACACCTTTTGATGAGATTAGCAAACCCTTACCATCAAATGCTGTGTGGAAAAAGGTCCACCTACGTAGTGGCTATGGAAAAAAGGAGAAGCAATACACTCAGGGTTACACTTTGACTGATGAACCTCTCTGTAAACTCTGCCAAACACCATGCGAGAGCCCCAATGCCAAGGAACCTGAATATTTTGAAGACCTGTTCTGTAATCTTGATTGTTATGGAGAGTACCGTATCCGAACTAGTAACAGATCTATTCGTCACGAACTTTTTCAACTAGAACGCGGTGTCTGTGCAAATTGTCAGTTGGACTGTCATAAACTTGTGGAGCACATAAGACCTTTATCTGATGTAAACCGGAGGCAGTATATTGAAAAGTTTGCACCAAGAGTGGCAAGGTTGAAGAAGTTGCTTGAGAGGCTTGTTAAGGATCCAACAGAGGGAAATGCATGGCATGCAGATCACCTTGTCCCTGTCTATCTTGGTGGAGGTGAATGCAGGCTAGAGAACATGAGGACTCTGTGTGTGGCCTGTCATTCTGATGTTACTAGAGCACAATGTGCTGAACGGCGGTCAACAAGGAGCAAGGCTAAGAAACAACTGAAAGCAATCATGAATGACCTTAAAAACAAAGGCACAGAGATTAACCCGAAGGACCAGGGGGATCCAGAGGCAGAGGAGAATTTGAGTGATGACGAACTTTTAGTCAAGGTTCCTGGGAGCGCCTACTCATTAGCAAATCATGGGGATGCTACAACGATGAATGAAGACTTGGAAGAGCCCTCCAATAGTGAGAAGATTCCCAGAGTAGAAAAATTCACATATACGTCTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HNH Helicase_C SNF2_N

Protein Analysis

877

Amino Acids

98.64

Weight (kDa)

6.15

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 153 - 254 2.3e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1800
AccII CGCG 2 cut(s) 148, 2082
AciI CCGC 5 cut(s) 899, 1575, 1798, 2082, 2368
AclWI GGATC 4 cut(s) 2222, 2235, 2456, 2469
AcoI YGGCCR 1 cut(s) 1354
AcsI RAATTY 7 cut(s) 38, 605, 1534, 1603, 1646, 2479, 2612
AcuI CTGAAG 4 cut(s) 285, 716, 1434, 1799
AdeI CACNNNGTG 1 cut(s) 2357
AfaI GTAC 6 cut(s) 115, 228, 507, 519, 1105, 2027
AfiI CCNNNNNNNGG 4 cut(s) 585, 1016, 1376, 2237
AflIII ACRYGT 4 cut(s) 135, 178, 688, 944
AhlI ACTAGT 1 cut(s) 2039
AjnI CCWGG 4 cut(s) 10, 1009, 2454, 2512
AjuI GAANNNNNNNTTGG 2 cut(s) 2552, 2584
Alw21I GWGCWC 3 cut(s) 751, 2130, 2353
Alw26I GTCTC 5 cut(s) 192, 917, 1162, 1214, 1278
AlwI GGATC 4 cut(s) 2222, 2235, 2456, 2469
AoxI GGCC 6 cut(s) 8, 1354, 1520, 1673, 1783, 2325
ApeKI GCWGC 4 cut(s) 260, 339, 352, 1020
ApoI RAATTY 7 cut(s) 38, 605, 1534, 1603, 1646, 2479, 2612
ArsI GACNNNNNNTTYG 4 cut(s) 440, 472, 2487, 2519
AseI ATTAAT 1 cut(s) 1694
Asp700I GAANNNNTTC 3 cut(s) 1395, 1597, 2067
AspLEI GCGC 3 cut(s) 849, 1797, 2522
AspS9I GGNCC 4 cut(s) 1674, 1783, 1858, 2452
AsuHPI GGTGA 7 cut(s) 353, 488, 708, 950, 1468, 2252, 2300
AsuNHI GCTAGC 1 cut(s) 385
AvaII GGWCC 2 cut(s) 1858, 2452
BalI TGGCCA 1 cut(s) 1356
BamHI GGATCC 2 cut(s) 2227, 2461
BanII GRGCYC 3 cut(s) 751, 1964, 2580
BarI GAAGNNNNNNTAC 2 cut(s) 1428, 1460
BbsI GAAGAC 4 cut(s) 688, 1323, 1998, 2571
Bbv12I GWGCWC 3 cut(s) 751, 2130, 2353
BbvCI CCTCAGC 1 cut(s) 738
BbvI GCAGC 4 cut(s) 247, 326, 364, 1007
BccI CCATC 7 cut(s) 431, 557, 727, 1103, 1346, 1379, 1843
BceAI ACGGC 2 cut(s) 834, 2381
BciT130I CCWGG 4 cut(s) 12, 1011, 2456, 2514
BciVI GTATCC 2 cut(s) 796, 2042
BcoDI GTCTC 5 cut(s) 192, 917, 1162, 1214, 1278
BcuI ACTAGT 1 cut(s) 2039
BfaI CTAG 6 cut(s) 386, 2040, 2075, 2300, 2346, 2632
BfoI RGCGCY 2 cut(s) 1798, 2523
BfuI GTATCC 2 cut(s) 796, 2042
BglII AGATCT 2 cut(s) 1085, 2048
BisI GCNGC 6 cut(s) 261, 340, 353, 1021, 1575, 1798
BlsI GCNGC 6 cut(s) 262, 341, 354, 1022, 1576, 1799
BmcAI AGTACT 1 cut(s) 507
Bme1390I CCNGG 4 cut(s) 12, 1011, 2456, 2514
Bme18I GGWCC 2 cut(s) 1858, 2452
BmgT120I GGNCC 4 cut(s) 1674, 1783, 1858, 2452
BmiI GGNNCC 4 cut(s) 1977, 2229, 2463, 2511
BmrFI CCNGG 4 cut(s) 12, 1011, 2456, 2514
BmsI GCATC 6 cut(s) 49, 717, 871, 1429, 1503, 2536
BmtI GCTAGC 1 cut(s) 389
BoxI GACNNNNGTC 1 cut(s) 1244
BpiI GAAGAC 4 cut(s) 688, 1323, 1998, 2571
Bpu10I CCTNAGC 2 cut(s) 738, 1029
BpuEI CTTGAG 2 cut(s) 1574, 2231
BsaAI YACGTR 1 cut(s) 1868
BsaI GGTCTC 1 cut(s) 1162
BsaJI CCNNGG 5 cut(s) 82, 1517, 1971, 2455, 2513
BsaWI WCCGGW 1 cut(s) 2154
Bsc4I CCNNNNNNNGG 4 cut(s) 585, 1016, 1376, 2237
Bse1I ACTGG 4 cut(s) 419, 465, 1296, 1305
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 4 cut(s) 12, 1011, 2456, 2514
BseDI CCNNGG 5 cut(s) 82, 1517, 1971, 2455, 2513
BseGI GGATG 4 cut(s) 319, 415, 1153, 2551
BseLI CCNNNNNNNGG 4 cut(s) 585, 1016, 1376, 2237
BseMI GCAATG 1 cut(s) 423
BseNI ACTGG 4 cut(s) 419, 465, 1296, 1305
BseRI GAGGAG 2 cut(s) 140, 2489
BseXI GCAGC 4 cut(s) 247, 326, 364, 1007
BseYI CCCAGC 3 cut(s) 19, 389, 1621
Bsh1236I CGCG 2 cut(s) 148, 2082
BshFI GGCC 6 cut(s) 10, 1356, 1522, 1675, 1785, 2327
BsiHKAI GWGCWC 3 cut(s) 751, 2130, 2353
BsiSI CCGG 1 cut(s) 2155
BslFI GGGAC 4 cut(s) 41, 1252, 1785, 2252
BslI CCNNNNNNNGG 4 cut(s) 585, 1016, 1376, 2237
BsmAI GTCTC 5 cut(s) 192, 917, 1162, 1214, 1278
BsmBI CGTCTC 1 cut(s) 1278
BsmFI GGGAC 4 cut(s) 41, 1252, 1785, 2252
BsmI GAATGC 1 cut(s) 2297
BsnI GGCC 6 cut(s) 10, 1356, 1522, 1675, 1785, 2327
Bso31I GGTCTC 1 cut(s) 1162
Bsp1286I GDGCHC 5 cut(s) 751, 1964, 2130, 2353, 2580
Bsp143I GATC 6 cut(s) 1085, 1371, 2048, 2227, 2257, 2461
BspACI CCGC 5 cut(s) 899, 1575, 1798, 2082, 2368
BspANI GGCC 6 cut(s) 10, 1356, 1522, 1675, 1785, 2327
BspFNI CGCG 2 cut(s) 148, 2082
BspHI TCATGA 1 cut(s) 2409
BspLI GGNNCC 4 cut(s) 1977, 2229, 2463, 2511
BspOI GCTAGC 1 cut(s) 389
BspPI GGATC 4 cut(s) 2222, 2235, 2456, 2469
BspQI GCTCTTC 2 cut(s) 1449, 2568
BspTNI GGTCTC 1 cut(s) 1162
BsrBI CCGCTC 1 cut(s) 1800
BsrDI GCAATG 1 cut(s) 423
BsrI ACTGG 4 cut(s) 419, 465, 1296, 1305
BssECI CCNNGG 5 cut(s) 82, 1517, 1971, 2455, 2513
BssMI GATC 6 cut(s) 1085, 1371, 2048, 2227, 2257, 2461
BssT1I CCWWGG 3 cut(s) 82, 1517, 1971
Bst2UI CCWGG 4 cut(s) 12, 1011, 2456, 2514
Bst4CI ACNGT 7 cut(s) 56, 154, 1181, 1243, 1726, 2030, 2111
Bst6I CTCTTC 2 cut(s) 1449, 2568
BstAPI GCANNNNNTGC 3 cut(s) 260, 2252, 2357
BstBAI YACGTR 1 cut(s) 1868
BstC8I GCNNGC 5 cut(s) 387, 1635, 1793, 2253, 2297
BstF5I GGATG 4 cut(s) 319, 415, 1153, 2551
BstFNI CGCG 2 cut(s) 148, 2082
BstH2I RGCGCY 2 cut(s) 1798, 2523
BstHHI GCGC 3 cut(s) 849, 1797, 2522
BstKTI GATC 6 cut(s) 1088, 1374, 2051, 2230, 2260, 2464
BstMAI GTCTC 5 cut(s) 192, 917, 1162, 1214, 1278
BstMBI GATC 6 cut(s) 1085, 1371, 2048, 2227, 2257, 2461
BstMWI GCNNNNNNNGC 7 cut(s) 16, 260, 386, 1797, 2215, 2252, 2357
BstNI CCWGG 4 cut(s) 12, 1011, 2456, 2514
BstNSI RCATGY 6 cut(s) 139, 182, 692, 948, 1637, 2255
BstPAI GACNNNNGTC 1 cut(s) 1244
BstSCI CCNGG 4 cut(s) 10, 1009, 2454, 2512
BstSNI TACGTA 1 cut(s) 1868
BstUI CGCG 2 cut(s) 148, 2082
BstV1I GCAGC 4 cut(s) 247, 326, 364, 1007
BstV2I GAAGAC 4 cut(s) 688, 1323, 1998, 2571
BstX2I RGATCY 4 cut(s) 1085, 2048, 2227, 2461
BstYI RGATCY 4 cut(s) 1085, 2048, 2227, 2461
BsuI GTATCC 2 cut(s) 796, 2042
BsuRI GGCC 6 cut(s) 10, 1356, 1522, 1675, 1785, 2327
BtsCI GGATG 4 cut(s) 319, 415, 1153, 2551
BtsI GCAGTG 1 cut(s) 1279
BtsIMutI CAGTG 2 cut(s) 1279, 1298
Cac8I GCNNGC 5 cut(s) 387, 1635, 1793, 2253, 2297
CciI TCATGA 1 cut(s) 2409
CfoI GCGC 3 cut(s) 849, 1797, 2522
Cfr13I GGNCC 4 cut(s) 1674, 1783, 1858, 2452
Csp6I GTAC 6 cut(s) 114, 227, 506, 518, 1104, 2026
CviQI GTAC 6 cut(s) 114, 227, 506, 518, 1104, 2026
DpnI GATC 6 cut(s) 1087, 1373, 2050, 2229, 2259, 2463
DpnII GATC 6 cut(s) 1085, 1371, 2048, 2227, 2257, 2461
DraI TTTAAA 1 cut(s) 37
DraIII CACNNNGTG 1 cut(s) 2357
EaeI YGGCCR 1 cut(s) 1354
Eam1104I CTCTTC 2 cut(s) 1449, 2568
EarI CTCTTC 2 cut(s) 1449, 2568
Ecl136II GAGCTC 1 cut(s) 749
Eco105I TACGTA 1 cut(s) 1868
Eco130I CCWWGG 3 cut(s) 82, 1517, 1971
Eco24I GRGCYC 3 cut(s) 751, 1964, 2580
Eco31I GGTCTC 1 cut(s) 1162
Eco47I GGWCC 2 cut(s) 1858, 2452
Eco53kI GAGCTC 1 cut(s) 749
Eco57I CTGAAG 4 cut(s) 285, 716, 1434, 1799
EcoICRI GAGCTC 1 cut(s) 749
EcoRI GAATTC 1 cut(s) 1646
EcoRII CCWGG 4 cut(s) 10, 1009, 2454, 2512
EcoT14I CCWWGG 3 cut(s) 82, 1517, 1971
EcoT22I ATGCAT 1 cut(s) 2248
EcoT38I GRGCYC 3 cut(s) 751, 1964, 2580
ErhI CCWWGG 3 cut(s) 82, 1517, 1971
Esp3I CGTCTC 1 cut(s) 1278
FaqI GGGAC 4 cut(s) 41, 1252, 1785, 2252
Fnu4HI GCNGC 6 cut(s) 261, 340, 353, 1021, 1575, 1798
FokI GGATG 4 cut(s) 326, 402, 1160, 2558
FriOI GRGCYC 3 cut(s) 751, 1964, 2580
Fsp4HI GCNGC 6 cut(s) 261, 340, 353, 1021, 1575, 1798
FspBI CTAG 6 cut(s) 386, 2040, 2075, 2300, 2346, 2632
GlaI GCGC 3 cut(s) 848, 1796, 2521
GluI GCNGC 6 cut(s) 261, 340, 353, 1021, 1575, 1798
GsaI CCCAGC 3 cut(s) 23, 393, 1625
HaeII RGCGCY 2 cut(s) 1798, 2523
HaeIII GGCC 6 cut(s) 10, 1356, 1522, 1675, 1785, 2327
HapII CCGG 1 cut(s) 2155
HhaI GCGC 3 cut(s) 849, 1797, 2522
Hin6I GCGC 3 cut(s) 847, 1795, 2520
HinP1I GCGC 3 cut(s) 847, 1795, 2520
HincII GTYRAC 2 cut(s) 778, 2373
HindII GTYRAC 2 cut(s) 778, 2373
HpaII CCGG 1 cut(s) 2155
HphI GGTGA 7 cut(s) 353, 488, 708, 950, 1468, 2252, 2300
Hpy166II GTNNAC 5 cut(s) 778, 1861, 1937, 2152, 2373
Hpy8I GTNNAC 5 cut(s) 778, 1861, 1937, 2152, 2373
HpyAV CCTTC 8 cut(s) 18, 166, 793, 1229, 1412, 1742, 1774, 2443
HpyCH4III ACNGT 7 cut(s) 56, 154, 1181, 1243, 1726, 2030, 2111
HpyCH4IV ACGT 3 cut(s) 166, 1867, 2624
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 260, 386, 1797, 2215, 2252, 2357
HpySE526I ACGT 3 cut(s) 166, 1867, 2624
HspAI GCGC 3 cut(s) 847, 1795, 2520
Kzo9I GATC 6 cut(s) 1085, 1371, 2048, 2227, 2257, 2461
LguI GCTCTTC 2 cut(s) 1449, 2568
LmnI GCTCC 4 cut(s) 703, 2125, 2379, 2517
Lsp1109I GCAGC 4 cut(s) 247, 326, 364, 1007
LweI GCATC 6 cut(s) 49, 717, 871, 1429, 1503, 2536
MaeI CTAG 6 cut(s) 386, 2040, 2075, 2300, 2346, 2632
MaeII ACGT 3 cut(s) 166, 1867, 2624
MaeIII GTNAC 9 cut(s) 138, 281, 714, 816, 1557, 1907, 2042, 2057, 2341
MalI GATC 6 cut(s) 1087, 1373, 2050, 2229, 2259, 2463
MbiI CCGCTC 1 cut(s) 1800
MboI GATC 6 cut(s) 1085, 1371, 2048, 2227, 2257, 2461
MfeI CAATTG 1 cut(s) 1404
MflI RGATCY 4 cut(s) 1085, 2048, 2227, 2461
MhlI GDGCHC 5 cut(s) 751, 1964, 2130, 2353, 2580
MlsI TGGCCA 1 cut(s) 1356
MluNI TGGCCA 1 cut(s) 1356
MlyI GAGTC 7 cut(s) 289, 464, 824, 1058, 1468, 1565, 2308
MmeI TCCRAC 5 cut(s) 438, 634, 1182, 2085, 2255
Mox20I TGGCCA 1 cut(s) 1356
Mph1103I ATGCAT 1 cut(s) 2248
MroXI GAANNNNTTC 3 cut(s) 1395, 1597, 2067
MscI TGGCCA 1 cut(s) 1356
MslI CAYNNNNRTG 3 cut(s) 312, 2337, 2544
Msp20I TGGCCA 1 cut(s) 1356
MspA1I CMGCKG 1 cut(s) 1577
MspI CCGG 1 cut(s) 2155
MspR9I CCNGG 4 cut(s) 12, 1011, 2456, 2514
MunI CAATTG 1 cut(s) 1404
Mva1269I GAATGC 1 cut(s) 2297
MvaI CCWGG 4 cut(s) 12, 1011, 2456, 2514
MvnI CGCG 2 cut(s) 148, 2082
MwoI GCNNNNNNNGC 7 cut(s) 16, 260, 386, 1797, 2215, 2252, 2357
NdeII GATC 6 cut(s) 1085, 1371, 2048, 2227, 2257, 2461
NheI GCTAGC 1 cut(s) 385
NlaIV GGNNCC 4 cut(s) 1977, 2229, 2463, 2511
NmuCI GTSAC 5 cut(s) 281, 714, 816, 1557, 2057
NsiI ATGCAT 1 cut(s) 2248
NspI RCATGY 6 cut(s) 139, 182, 692, 948, 1637, 2255
PaeI GCATGC 2 cut(s) 1637, 2255
PagI TCATGA 1 cut(s) 2409
PciI ACATGT 4 cut(s) 135, 178, 688, 944
PciSI GCTCTTC 2 cut(s) 1449, 2568
PctI GAATGC 1 cut(s) 2297
PdmI GAANNNNTTC 3 cut(s) 1395, 1597, 2067
PfeI GAWTC 6 cut(s) 461, 1295, 1323, 1476, 1508, 2596
PkrI GCNGC 6 cut(s) 262, 341, 354, 1022, 1576, 1799
PleI GAGTC 7 cut(s) 288, 463, 823, 1057, 1467, 1564, 2308
PpsI GAGTC 7 cut(s) 288, 463, 823, 1057, 1467, 1564, 2308
Ppu21I YACGTR 1 cut(s) 1868
PscI ACATGT 4 cut(s) 135, 178, 688, 944
PshAI GACNNNNGTC 1 cut(s) 1244
PshBI ATTAAT 1 cut(s) 1694
Psp124BI GAGCTC 1 cut(s) 751
Psp6I CCWGG 4 cut(s) 10, 1009, 2454, 2512
PspFI CCCAGC 3 cut(s) 19, 389, 1621
PspGI CCWGG 4 cut(s) 10, 1009, 2454, 2512
PspN4I GGNNCC 4 cut(s) 1977, 2229, 2463, 2511
PspPI GGNCC 4 cut(s) 1674, 1783, 1858, 2452
PsuI RGATCY 4 cut(s) 1085, 2048, 2227, 2461
RsaI GTAC 6 cut(s) 115, 228, 507, 519, 1105, 2027
RsaNI GTAC 6 cut(s) 114, 227, 506, 518, 1104, 2026
RseI CAYNNNNRTG 3 cut(s) 312, 2337, 2544
SacI GAGCTC 1 cut(s) 751
SapI GCTCTTC 2 cut(s) 1449, 2568
SatI GCNGC 6 cut(s) 261, 340, 353, 1021, 1575, 1798
Sau3AI GATC 6 cut(s) 1085, 1371, 2048, 2227, 2257, 2461
Sau96I GGNCC 4 cut(s) 1674, 1783, 1858, 2452
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 7 cut(s) 289, 464, 824, 1058, 1468, 1565, 2308
ScrFI CCNGG 4 cut(s) 12, 1011, 2456, 2514
SduI GDGCHC 5 cut(s) 751, 1964, 2130, 2353, 2580
SfaNI GCATC 6 cut(s) 49, 717, 871, 1429, 1503, 2536
SinI GGWCC 2 cut(s) 1858, 2452
SmiMI CAYNNNNRTG 3 cut(s) 312, 2337, 2544
SmlI CTYRAG 2 cut(s) 1553, 2210
SmoI CTYRAG 2 cut(s) 1553, 2210
SnaBI TACGTA 1 cut(s) 1868
SpeI ACTAGT 1 cut(s) 2039
SphI GCATGC 2 cut(s) 1637, 2255
SsiI CCGC 5 cut(s) 899, 1575, 1798, 2082, 2368
SspI AATATT 2 cut(s) 486, 1985
SspMI CTAG 6 cut(s) 386, 2040, 2075, 2300, 2346, 2632
SstI GAGCTC 1 cut(s) 751
StyD4I CCNGG 4 cut(s) 10, 1009, 2454, 2512
StyI CCWWGG 3 cut(s) 82, 1517, 1971
TaaI ACNGT 7 cut(s) 56, 154, 1181, 1243, 1726, 2030, 2111
TaiI ACGT 3 cut(s) 169, 1870, 2627
TaqI TCGA 1 cut(s) 1447
TatI WGTACW 3 cut(s) 226, 505, 517
TauI GCSGC 2 cut(s) 1577, 1800
TfiI GAWTC 6 cut(s) 461, 1295, 1323, 1476, 1508, 2596
TscAI CASTG 2 cut(s) 1279, 1305
TseFI GTSAC 5 cut(s) 281, 714, 816, 1557, 2057
TseI GCWGC 4 cut(s) 260, 339, 352, 1020
Tsp45I GTSAC 5 cut(s) 281, 714, 816, 1557, 2057
TspGWI ACGGA 3 cut(s) 528, 1302, 1335
TspRI CASTG 2 cut(s) 1279, 1305
VpaK11BI GGWCC 2 cut(s) 1858, 2452
VspI ATTAAT 1 cut(s) 1694
XapI RAATTY 7 cut(s) 38, 605, 1534, 1603, 1646, 2479, 2612
XceI RCATGY 6 cut(s) 139, 182, 692, 948, 1637, 2255
XcmI CCANNNNNNNNNTGG 1 cut(s) 1868
XmnI GAANNNNTTC 3 cut(s) 1395, 1597, 2067
XspI CTAG 6 cut(s) 386, 2040, 2075, 2300, 2346, 2632
ZrmI AGTACT 1 cut(s) 507
Zsp2I ATGCAT 1 cut(s) 2248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.