FvH4_5g14990

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001880
Physical Location & Seq
Forward (+)
8502127 .. 8504186
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14990.t1

Sequence Viewer

Length: 363 bp
ATGGTGCAGAGGCTCACATACCGTTCGCGCCATAGCTATGCTACCAAGTCCAATCAGCACCGGATCGTCAAAACCCCTGGTGGGAAGCTTGTGTATCAGACCACTAAGAAGAGAGCAAGTGGGCCCAAGTGTCCTGTTACTGGAAAGAGAATCCAAGGGATTCCTCACTTGAGACCTGCTGAATATAAGAGGTCTAGACTATCAAGGAACAGGAGGACCGTGAATCGTGCCTACGGCGGGGTTCTGTCTGGTGGAGCTGTTAGGGAGAGGATAATTCGAGCCTTTTTGGTGGAAGAGCAGAAGATCGTGAAGAAGGTATTGAAGATTCAGAAGGCCAAAGAAAGAGCAGCATCAAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34e

Protein Analysis

121

Amino Acids

13.67

Weight (kDa)

11.67

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34e PF01199 1 - 96 2.1e-38 Ribosomal protein L34e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 184
AccII CGCG 1 cut(s) 28
AciI CCGC 1 cut(s) 237
AclWI GGATC 1 cut(s) 71
AfiI CCNNNNNNNGG 3 cut(s) 81, 140, 237
AgsI TTSAA 1 cut(s) 322
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 4 cut(s) 36, 88, 257, 360
AluI AGCT 4 cut(s) 36, 88, 257, 360
Alw26I GTCTC 1 cut(s) 166
AlwI GGATC 1 cut(s) 71
AoxI GGCC 2 cut(s) 122, 333
ApaI GGGCCC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 347
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 3 cut(s) 122, 123, 216
AvaII GGWCC 1 cut(s) 216
BaeGI GKGCMC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 126
BbvI GCAGC 1 cut(s) 359
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 1 cut(s) 78
BcoDI GTCTC 1 cut(s) 166
BfaI CTAG 1 cut(s) 195
BfuAI ACCTGC 1 cut(s) 184
BisI GCNGC 1 cut(s) 348
BlsI GCNGC 1 cut(s) 349
Bme1390I CCNGG 1 cut(s) 78
Bme18I GGWCC 1 cut(s) 216
BmgT120I GGNCC 3 cut(s) 122, 123, 216
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 78
BmsI GCATC 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 190
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 76, 154
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 140, 237
Bse1I ACTGG 1 cut(s) 145
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 76, 154
BseLI CCNNNNNNNGG 3 cut(s) 81, 140, 237
BseNI ACTGG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 124, 335
BsiSI CCGG 1 cut(s) 61
BslI CCNNNNNNNGG 3 cut(s) 81, 140, 237
BsmAI GTCTC 1 cut(s) 166
BsnI GGCC 2 cut(s) 124, 335
Bso31I GGTCTC 1 cut(s) 166
Bsp120I GGGCCC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 63, 303
BspACI CCGC 1 cut(s) 237
BspANI GGCC 2 cut(s) 124, 335
BspFNI CGCG 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 184
BspPI GGATC 1 cut(s) 71
BspQI GCTCTTC 1 cut(s) 288
BspTNI GGTCTC 1 cut(s) 166
BsrI ACTGG 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 76, 154
BssMI GATC 2 cut(s) 63, 303
BssT1I CCWWGG 1 cut(s) 154
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 2 cut(s) 23, 220
Bst6I CTCTTC 2 cut(s) 104, 288
BstDEI CTNAG 1 cut(s) 105
BstFNI CGCG 1 cut(s) 28
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 2 cut(s) 66, 306
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 2 cut(s) 63, 303
BstNI CCWGG 1 cut(s) 78
BstSCI CCNGG 1 cut(s) 76
BstSLI GKGCMC 1 cut(s) 126
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 359
BsuRI GGCC 2 cut(s) 124, 335
BveI ACCTGC 1 cut(s) 184
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 3 cut(s) 122, 123, 216
CviJI RGCY 8 cut(s) 13, 36, 88, 124, 257, 281, 335, 360
CviKI_1 RGCY 8 cut(s) 13, 36, 88, 124, 257, 281, 335, 360
DdeI CTNAG 1 cut(s) 105
DpnI GATC 2 cut(s) 65, 305
DpnII GATC 2 cut(s) 63, 303
Eam1104I CTCTTC 2 cut(s) 104, 288
EarI CTCTTC 2 cut(s) 104, 288
Eco130I CCWWGG 1 cut(s) 154
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 154
FaiI YATR 4 cut(s) 19, 33, 39, 186
FauI CCCGC 1 cut(s) 230
Fnu4HI GCNGC 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 1 cut(s) 348
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 29
GluI GCNGC 1 cut(s) 348
HaeIII GGCC 2 cut(s) 124, 335
HapII CCGG 1 cut(s) 61
HhaI GCGC 1 cut(s) 30
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 4 cut(s) 150, 160, 223, 325
HpaII CCGG 1 cut(s) 61
Hpy188I TCNGA 2 cut(s) 99, 330
Hpy188III TCNNGA 2 cut(s) 195, 307
HpyAV CCTTC 2 cut(s) 307, 325
HpyCH4III ACNGT 2 cut(s) 23, 220
HpyCH4V TGCA 1 cut(s) 7
HpyF3I CTNAG 1 cut(s) 105
HspAI GCGC 1 cut(s) 28
Kzo9I GATC 2 cut(s) 63, 303
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 8 cut(s) 63, 74, 90, 126, 147, 189, 196, 234
Lsp1109I GCAGC 1 cut(s) 359
LweI GCATC 1 cut(s) 359
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 136
MalI GATC 2 cut(s) 65, 305
MboI GATC 2 cut(s) 63, 303
MboII GAAGA 5 cut(s) 121, 305, 313, 322, 334
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 1 cut(s) 273
MnlI CCTC 5 cut(s) 3, 174, 183, 207, 261
MslI CAYNNNNRTG 1 cut(s) 36
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 1 cut(s) 78
MvaI CCWGG 1 cut(s) 78
MvnI CGCG 1 cut(s) 28
NdeII GATC 2 cut(s) 63, 303
NlaIV GGNNCC 1 cut(s) 124
PciSI GCTCTTC 1 cut(s) 288
PfeI GAWTC 4 cut(s) 150, 160, 223, 325
PkrI GCNGC 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 124
PspOMI GGGCCC 1 cut(s) 122
PspPI GGNCC 3 cut(s) 122, 123, 216
RseI CAYNNNNRTG 1 cut(s) 36
SapI GCTCTTC 1 cut(s) 288
SatI GCNGC 1 cut(s) 348
Sau3AI GATC 2 cut(s) 63, 303
Sau96I GGNCC 3 cut(s) 122, 123, 216
ScrFI CCNGG 1 cut(s) 78
SduI GDGCHC 1 cut(s) 126
SetI ASST 7 cut(s) 38, 90, 178, 194, 259, 318, 362
SfaNI GCATC 1 cut(s) 359
SinI GGWCC 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 1 cut(s) 273
SsiI CCGC 1 cut(s) 237
SspMI CTAG 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 76
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 2 cut(s) 23, 220
TaqI TCGA 1 cut(s) 277
TasI AATT 1 cut(s) 273
TfiI GAWTC 4 cut(s) 150, 160, 223, 325
TseI GCWGC 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 216
XbaI TCTAGA 1 cut(s) 194
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.