MD16G1073200.v1.1

60S ribosomal protein L34-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001880
Physical Location & Seq
Forward (+)
5139448 .. 5141489
2042 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1073200.v1.1.491

Sequence Viewer

Length: 366 bp
ATGGTGCAGCGTCTCACATATCGCGCCCGGCACAGCTACGCCACCAAATCCAACCAGCACAGGATCGTCAAAACCCCTGGAGGGAAGCTAGTGTACCAGACCACTAAGAAGAGGGCAAGTGGCCCCAAGTGCCCTGTTACTGGCAAGAGAATTCAAGGGATCCCTCACTTGAGGCCTACCGAGTATAAGAGGTCCAGATTATCTAGGAATCGGAGGACTGTTAACCGTGCTTATGGAGGTGTGTTGTCTGGAGGTGCAGTCAAGGAAAGGATCATCAGGGCCTTTCTGGTGGAGGAACAAAAGATCGTGAAAAAGGTGTTGAAGATTCAAAAGACAAAGGAAAAGCAAGCTGCAAAGACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34e

Protein Analysis

122

Amino Acids

13.84

Weight (kDa)

11.51

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34e PF01199 1 - 96 4.3e-38 Ribosomal protein L34e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 24
AclWI GGATC 4 cut(s) 71, 154, 167, 278
AcsI RAATTY 1 cut(s) 150
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 81, 140
AgsI TTSAA 3 cut(s) 155, 322, 329
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 3 cut(s) 36, 88, 350
AluI AGCT 3 cut(s) 36, 88, 350
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 4 cut(s) 71, 154, 167, 278
AoxI GGCC 3 cut(s) 121, 173, 279
ApeKI GCWGC 2 cut(s) 7, 350
ApoI RAATTY 1 cut(s) 150
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 3 cut(s) 122, 192, 279
AsuC2I CCSGG 1 cut(s) 28
AvaII GGWCC 1 cut(s) 192
BaeGI GKGCMC 1 cut(s) 134
BamHI GGATCC 1 cut(s) 159
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbvI GCAGC 2 cut(s) 19, 337
BciT130I CCWGG 1 cut(s) 78
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 2 cut(s) 89, 204
BisI GCNGC 2 cut(s) 8, 351
BlsI GCNGC 2 cut(s) 9, 352
Bme1390I CCNGG 2 cut(s) 28, 78
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 3 cut(s) 122, 192, 279
BmiI GGNNCC 2 cut(s) 124, 161
BmrFI CCNGG 2 cut(s) 28, 78
BpmI CTGGAG 2 cut(s) 99, 270
BpuEI CTTGAG 1 cut(s) 190
BpuMI CCSGG 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 140
Bse1I ACTGG 1 cut(s) 145
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 76
BseLI CCNNNNNNNGG 2 cut(s) 81, 140
BseNI ACTGG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 134
BseXI GCAGC 2 cut(s) 19, 337
BsgI GTGCAG 2 cut(s) 26, 276
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 3 cut(s) 123, 175, 281
BsiSI CCGG 1 cut(s) 28
BslI CCNNNNNNNGG 2 cut(s) 81, 140
BsmAI GTCTC 1 cut(s) 17
BsmBI CGTCTC 1 cut(s) 17
BsnI GGCC 3 cut(s) 123, 175, 281
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 4 cut(s) 63, 159, 270, 303
BspANI GGCC 3 cut(s) 123, 175, 281
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 124, 161
BspPI GGATC 4 cut(s) 71, 154, 167, 278
BsrI ACTGG 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 4 cut(s) 63, 159, 270, 303
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 2 cut(s) 220, 227
Bst6I CTCTTC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 105
BstFNI CGCG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 4 cut(s) 66, 162, 273, 306
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 4 cut(s) 63, 159, 270, 303
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstNI CCWGG 1 cut(s) 78
BstSCI CCNGG 2 cut(s) 26, 76
BstSLI GKGCMC 1 cut(s) 134
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 19, 337
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 3 cut(s) 123, 175, 281
Cac8I GCNNGC 1 cut(s) 348
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 3 cut(s) 122, 192, 279
Csp6I GTAC 1 cut(s) 94
CviJI RGCY 6 cut(s) 36, 88, 123, 175, 281, 350
CviKI_1 RGCY 6 cut(s) 36, 88, 123, 175, 281, 350
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 1 cut(s) 105
DpnI GATC 4 cut(s) 65, 161, 272, 305
DpnII GATC 4 cut(s) 63, 159, 270, 303
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Eco147I AGGCCT 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 192
EcoO109I RGGNCCY 1 cut(s) 279
EcoRI GAATTC 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 17
FaiI YATR 3 cut(s) 19, 186, 234
Fnu4HI GCNGC 2 cut(s) 8, 351
Fsp4HI GCNGC 2 cut(s) 8, 351
FspBI CTAG 2 cut(s) 89, 204
GlaI GCGC 1 cut(s) 25
GluI GCNGC 2 cut(s) 8, 351
GsuI CTGGAG 2 cut(s) 99, 270
HaeIII GGCC 3 cut(s) 123, 175, 281
HapII CCGG 1 cut(s) 28
HhaI GCGC 1 cut(s) 26
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 2 cut(s) 208, 325
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 94, 223
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 3 cut(s) 195, 249, 307
Hpy8I GTNNAC 2 cut(s) 94, 223
HpyCH4III ACNGT 2 cut(s) 220, 227
HpyCH4V TGCA 3 cut(s) 7, 257, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 1 cut(s) 105
HspAI GCGC 1 cut(s) 24
KspAI GTTAAC 1 cut(s) 223
Kzo9I GATC 4 cut(s) 63, 159, 270, 303
Lsp1109I GCAGC 2 cut(s) 19, 337
MaeI CTAG 2 cut(s) 89, 204
MaeIII GTNAC 1 cut(s) 136
MalI GATC 4 cut(s) 65, 161, 272, 305
MboI GATC 4 cut(s) 63, 159, 270, 303
MboII GAAGA 2 cut(s) 121, 334
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 9 cut(s) 74, 105, 165, 174, 183, 207, 230, 245, 286
MseI TTAA 1 cut(s) 222
MspI CCGG 1 cut(s) 28
MspR9I CCNGG 2 cut(s) 28, 78
MvaI CCWGG 1 cut(s) 78
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 28
NdeII GATC 4 cut(s) 63, 159, 270, 303
NlaIV GGNNCC 2 cut(s) 124, 161
PceI AGGCCT 1 cut(s) 175
PfeI GAWTC 2 cut(s) 208, 325
PkrI GCNGC 2 cut(s) 9, 352
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 2 cut(s) 124, 161
PspPI GGNCC 3 cut(s) 122, 192, 279
PsuI RGATCY 1 cut(s) 159
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 2 cut(s) 8, 351
Sau3AI GATC 4 cut(s) 63, 159, 270, 303
Sau96I GGNCC 3 cut(s) 122, 192, 279
ScrFI CCNGG 2 cut(s) 28, 78
SduI GDGCHC 1 cut(s) 134
SetI ASST 7 cut(s) 38, 90, 194, 241, 256, 318, 352
SinI GGWCC 1 cut(s) 192
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 1 cut(s) 150
SseBI AGGCCT 1 cut(s) 175
SspMI CTAG 2 cut(s) 89, 204
StuI AGGCCT 1 cut(s) 175
StyD4I CCNGG 2 cut(s) 26, 76
TaaI ACNGT 2 cut(s) 220, 227
TasI AATT 1 cut(s) 150
TfiI GAWTC 2 cut(s) 208, 325
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TseI GCWGC 2 cut(s) 7, 350
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 1 cut(s) 150
XspI CTAG 2 cut(s) 89, 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.