MD13G1072400.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001880
Physical Location & Seq
Forward (+)
5093997 .. 5095950
1954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1072400.v1.1.491

Sequence Viewer

Length: 366 bp
ATGGTGCAGCGTCTCACGTATCGCGCCCGGCACAGCTACGCCACCAAATCCAACCAGCACAGGATCGTCAAAACCCCTGGAGGGAAGCTAGTTTACCAGACCACTAAGAAGAGGGCAAGCGGGCCCAAGTGCCCTGTTACCGGCAAGAGGATTCAAGGGATCCCTCACTTGAGGCCTACTGAGTATAAGAGGTCCAGATTATCTAGGAATCGGAGGACTGTTAACCGAGCTTATGGAGGTGTATTGTCTGGAGGTGCTGTCAAAGAAAGGATCATCAGGGCCTTTCTGGTGGAGGAACAGAAGATTGTGAAAAAAGTGTTGAAGATCCAGAAGGCAAAGGAAAAGCAAGCTTCAAAGACCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34e

Protein Analysis

122

Amino Acids

13.82

Weight (kDa)

11.51

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34e PF01199 1 - 96 4.3e-38 Ribosomal protein L34e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 24
AciI CCGC 1 cut(s) 120
AclWI GGATC 5 cut(s) 71, 154, 167, 278, 319
AfiI CCNNNNNNNGG 3 cut(s) 81, 140, 147
AgsI TTSAA 3 cut(s) 155, 322, 354
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 4 cut(s) 36, 88, 230, 350
AluI AGCT 4 cut(s) 36, 88, 230, 350
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 5 cut(s) 71, 154, 167, 278, 319
AoxI GGCC 3 cut(s) 122, 173, 279
ApaI GGGCCC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 7
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 4 cut(s) 122, 123, 192, 279
AsuC2I CCSGG 1 cut(s) 28
AvaII GGWCC 1 cut(s) 192
BaeGI GKGCMC 2 cut(s) 126, 134
BamHI GGATCC 1 cut(s) 159
BanII GRGCYC 1 cut(s) 126
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbvI GCAGC 1 cut(s) 19
BciT130I CCWGG 1 cut(s) 78
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 2 cut(s) 89, 204
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
Bme1390I CCNGG 2 cut(s) 28, 78
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 4 cut(s) 122, 123, 192, 279
BmiI GGNNCC 2 cut(s) 124, 161
BmrFI CCNGG 2 cut(s) 28, 78
BpmI CTGGAG 2 cut(s) 99, 270
BpuEI CTTGAG 1 cut(s) 190
BpuMI CCSGG 1 cut(s) 28
BsaAI YACGTR 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 140, 147
Bse118I RCCGGY 1 cut(s) 140
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 76
BseLI CCNNNNNNNGG 3 cut(s) 81, 140, 147
BseMII CTCAG 1 cut(s) 171
BseSI GKGCMC 2 cut(s) 126, 134
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 3 cut(s) 124, 175, 281
BsiSI CCGG 2 cut(s) 28, 141
BslI CCNNNNNNNGG 3 cut(s) 81, 140, 147
BsmAI GTCTC 1 cut(s) 17
BsmBI CGTCTC 1 cut(s) 17
BsnI GGCC 3 cut(s) 124, 175, 281
Bsp120I GGGCCC 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 126, 134
Bsp143I GATC 4 cut(s) 63, 159, 270, 324
BspACI CCGC 1 cut(s) 120
BspANI GGCC 3 cut(s) 124, 175, 281
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 124, 161
BspPI GGATC 5 cut(s) 71, 154, 167, 278, 319
BsrFI RCCGGY 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 4 cut(s) 63, 159, 270, 324
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 1 cut(s) 104
BstBAI YACGTR 1 cut(s) 18
BstC8I GCNNGC 3 cut(s) 118, 122, 348
BstDEI CTNAG 2 cut(s) 105, 180
BstFNI CGCG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 4 cut(s) 66, 162, 273, 327
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 4 cut(s) 63, 159, 270, 324
BstNI CCWGG 1 cut(s) 78
BstSCI CCNGG 2 cut(s) 26, 76
BstSLI GKGCMC 2 cut(s) 126, 134
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 19
BstX2I RGATCY 2 cut(s) 159, 324
BstYI RGATCY 2 cut(s) 159, 324
BsuRI GGCC 3 cut(s) 124, 175, 281
Cac8I GCNNGC 3 cut(s) 118, 122, 348
CfoI GCGC 1 cut(s) 26
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 4 cut(s) 122, 123, 192, 279
CviJI RGCY 7 cut(s) 36, 88, 124, 175, 230, 281, 350
CviKI_1 RGCY 7 cut(s) 36, 88, 124, 175, 230, 281, 350
DdeI CTNAG 2 cut(s) 105, 180
DpnI GATC 4 cut(s) 65, 161, 272, 326
DpnII GATC 4 cut(s) 63, 159, 270, 324
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Eco147I AGGCCT 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 192
EcoO109I RGGNCCY 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 126
Esp3I CGTCTC 1 cut(s) 17
FaiI YATR 2 cut(s) 186, 234
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 1 cut(s) 8
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 1 cut(s) 8
FspBI CTAG 2 cut(s) 89, 204
GlaI GCGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 8
GsuI CTGGAG 2 cut(s) 99, 270
HaeIII GGCC 3 cut(s) 124, 175, 281
HapII CCGG 2 cut(s) 28, 141
HhaI GCGC 1 cut(s) 26
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 2 cut(s) 151, 208
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 2 cut(s) 28, 141
Hpy166II GTNNAC 2 cut(s) 94, 223
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 3 cut(s) 195, 249, 328
Hpy8I GTNNAC 2 cut(s) 94, 223
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 1 cut(s) 7
HpyF3I CTNAG 2 cut(s) 105, 180
HpySE526I ACGT 1 cut(s) 17
HspAI GCGC 1 cut(s) 24
KspAI GTTAAC 1 cut(s) 223
Kzo9I GATC 4 cut(s) 63, 159, 270, 324
Lsp1109I GCAGC 1 cut(s) 19
MaeI CTAG 2 cut(s) 89, 204
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 1 cut(s) 136
MalI GATC 4 cut(s) 65, 161, 272, 326
MboI GATC 4 cut(s) 63, 159, 270, 324
MboII GAAGA 3 cut(s) 121, 313, 334
MflI RGATCY 2 cut(s) 159, 324
MhlI GDGCHC 2 cut(s) 126, 134
MmeI TCCRAC 1 cut(s) 75
MseI TTAA 1 cut(s) 222
MspI CCGG 2 cut(s) 28, 141
MspR9I CCNGG 2 cut(s) 28, 78
MvaI CCWGG 1 cut(s) 78
MvnI CGCG 1 cut(s) 24
NciI CCSGG 1 cut(s) 28
NdeII GATC 4 cut(s) 63, 159, 270, 324
NlaIV GGNNCC 2 cut(s) 124, 161
PceI AGGCCT 1 cut(s) 175
PfeI GAWTC 2 cut(s) 151, 208
PkrI GCNGC 1 cut(s) 9
Ppu21I YACGTR 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 2 cut(s) 124, 161
PspOMI GGGCCC 1 cut(s) 122
PspPI GGNCC 4 cut(s) 122, 123, 192, 279
PsuI RGATCY 2 cut(s) 159, 324
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 4 cut(s) 63, 159, 270, 324
Sau96I GGNCC 4 cut(s) 122, 123, 192, 279
ScrFI CCNGG 2 cut(s) 28, 78
SduI GDGCHC 2 cut(s) 126, 134
SetI ASST 8 cut(s) 20, 38, 90, 194, 232, 241, 256, 352
SinI GGWCC 1 cut(s) 192
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
SseBI AGGCCT 1 cut(s) 175
SsiI CCGC 1 cut(s) 120
SspMI CTAG 2 cut(s) 89, 204
StuI AGGCCT 1 cut(s) 175
StyD4I CCNGG 2 cut(s) 26, 76
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 1 cut(s) 20
TfiI GAWTC 2 cut(s) 151, 208
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TseI GCWGC 1 cut(s) 7
VpaK11BI GGWCC 1 cut(s) 192
XspI CTAG 2 cut(s) 89, 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.