MD09G1050600.v1.1

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001880
Physical Location & Seq
Reverse (-)
3360289 .. 3362180
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1050600.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGTGCAGCGGCTCACGTACCGGAAGCGACACAGCTACGCCACCAAGTCCAACCAGCACCGCATCGTCAAAACCCCCGGTGGGAAATTGATCTATCAGACCACCAAGAAGAGAGCCAGCGGCCCCAAATGCCCTGTTACTGGCAAGAGAATCCAAGGGATTCCTCACTTGAGACCGGCTGAGTACAAGAGGTCTAGACTATCTAGGAACCGCAGGACTGTGAACCGTGCCTATGGTGGTGTATTGTCTGGAGGTGCTGTTAAGGAGAGGATCATTCGAGCTTTCTTGGTGGAAGAGCAGAAAATTGTGAAGAAGGTTTTGAAGATCCAAAAGGCCAAGGAAAGGCAGGCGGAGAAAGGAGCTAGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34e

Protein Analysis

124

Amino Acids

14.08

Weight (kDa)

11.57

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34e PF01199 1 - 96 5.4e-38 Ribosomal protein L34e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 10, 61, 120, 211, 350
AclWI GGATC 2 cut(s) 278, 319
AfaI GTAC 2 cut(s) 20, 185
AfiI CCNNNNNNNGG 3 cut(s) 81, 140, 342
AgsI TTSAA 1 cut(s) 322
AluBI AGCT 4 cut(s) 36, 281, 362, 369
AluI AGCT 4 cut(s) 36, 281, 362, 369
Alw26I GTCTC 1 cut(s) 166
AlwI GGATC 2 cut(s) 278, 319
AoxI GGCC 2 cut(s) 121, 333
ApeKI GCWGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 1 cut(s) 78
BbvI GCAGC 1 cut(s) 19
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 1 cut(s) 166
BfaI CTAG 3 cut(s) 195, 204, 363
BisI GCNGC 3 cut(s) 8, 11, 121
BlsI GCNGC 3 cut(s) 9, 12, 122
Bme1390I CCNGG 1 cut(s) 78
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 2 cut(s) 124, 209
BmrFI CCNGG 1 cut(s) 78
BmsI GCATC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 190
BpuMI CCSGG 1 cut(s) 78
BsaAI YACGTR 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 3 cut(s) 76, 154, 336
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 140, 342
Bse118I RCCGGY 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 145
BseDI CCNNGG 3 cut(s) 76, 154, 336
BseLI CCNNNNNNNGG 3 cut(s) 81, 140, 342
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 145
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 2 cut(s) 123, 335
BsiSI CCGG 3 cut(s) 22, 78, 176
BslI CCNNNNNNNGG 3 cut(s) 81, 140, 342
BsmAI GTCTC 1 cut(s) 166
BsnI GGCC 2 cut(s) 123, 335
Bso31I GGTCTC 1 cut(s) 166
Bsp143I GATC 3 cut(s) 90, 270, 324
BspACI CCGC 5 cut(s) 10, 61, 120, 211, 350
BspANI GGCC 2 cut(s) 123, 335
BspCNI CTCAG 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 124, 209
BspPI GGATC 2 cut(s) 278, 319
BspQI GCTCTTC 1 cut(s) 288
BspTNI GGTCTC 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 175
BsrI ACTGG 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 3 cut(s) 76, 154, 336
BssMI GATC 3 cut(s) 90, 270, 324
BssT1I CCWWGG 2 cut(s) 154, 336
Bst4CI ACNGT 2 cut(s) 220, 227
Bst6I CTCTTC 2 cut(s) 104, 288
BstBAI YACGTR 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 118, 348
BstDEI CTNAG 1 cut(s) 180
BstKTI GATC 3 cut(s) 93, 273, 327
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 3 cut(s) 90, 270, 324
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 19
BstX2I RGATCY 1 cut(s) 324
BstYI RGATCY 1 cut(s) 324
BsuRI GGCC 2 cut(s) 123, 335
Cac8I GCNNGC 2 cut(s) 118, 348
Cfr10I RCCGGY 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 19, 184
CviJI RGCY 9 cut(s) 13, 36, 116, 123, 179, 281, 335, 362, 369
CviKI_1 RGCY 9 cut(s) 13, 36, 116, 123, 179, 281, 335, 362, 369
CviQI GTAC 2 cut(s) 19, 184
DdeI CTNAG 1 cut(s) 180
DpnI GATC 3 cut(s) 92, 272, 326
DpnII GATC 3 cut(s) 90, 270, 324
Eam1104I CTCTTC 2 cut(s) 104, 288
EarI CTCTTC 2 cut(s) 104, 288
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 2 cut(s) 154, 336
Eco31I GGTCTC 1 cut(s) 166
EcoT14I CCWWGG 2 cut(s) 154, 336
ErhI CCWWGG 2 cut(s) 154, 336
FaiI YATR 1 cut(s) 234
Fnu4HI GCNGC 3 cut(s) 8, 11, 121
Fsp4HI GCNGC 3 cut(s) 8, 11, 121
FspBI CTAG 3 cut(s) 195, 204, 363
GluI GCNGC 3 cut(s) 8, 11, 121
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 2 cut(s) 123, 335
HapII CCGG 3 cut(s) 22, 78, 176
HinfI GANTC 2 cut(s) 150, 160
HpaII CCGG 3 cut(s) 22, 78, 176
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 2 cut(s) 195, 249
Hpy8I GTNNAC 1 cut(s) 223
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 2 cut(s) 220, 227
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 1 cut(s) 7
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 1 cut(s) 180
HpySE526I ACGT 1 cut(s) 17
Kzo9I GATC 3 cut(s) 90, 270, 324
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 2 cut(s) 359, 366
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 1 cut(s) 72
MaeI CTAG 3 cut(s) 195, 204, 363
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 1 cut(s) 136
MalI GATC 3 cut(s) 92, 272, 326
MboI GATC 3 cut(s) 90, 270, 324
MboII GAAGA 4 cut(s) 121, 305, 322, 334
MflI RGATCY 1 cut(s) 324
MluCI AATT 2 cut(s) 86, 303
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 4 cut(s) 174, 183, 245, 261
MseI TTAA 1 cut(s) 261
MspA1I CMGCKG 2 cut(s) 10, 120
MspI CCGG 3 cut(s) 22, 78, 176
MspR9I CCNGG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 78
NdeII GATC 3 cut(s) 90, 270, 324
NlaIV GGNNCC 2 cut(s) 124, 209
PciSI GCTCTTC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 150, 160
PkrI GCNGC 3 cut(s) 9, 12, 122
Ppu21I YACGTR 1 cut(s) 18
PspN4I GGNNCC 2 cut(s) 124, 209
PspPI GGNCC 1 cut(s) 122
PsuI RGATCY 1 cut(s) 324
RsaI GTAC 2 cut(s) 20, 185
RsaNI GTAC 2 cut(s) 19, 184
SapI GCTCTTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 3 cut(s) 8, 11, 121
Sau3AI GATC 3 cut(s) 90, 270, 324
Sau96I GGNCC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 78
SetI ASST 8 cut(s) 20, 38, 194, 256, 283, 318, 364, 371
SfaNI GCATC 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 2 cut(s) 86, 303
SsiI CCGC 5 cut(s) 10, 61, 120, 211, 350
SspMI CTAG 3 cut(s) 195, 204, 363
StyD4I CCNGG 1 cut(s) 76
StyI CCWWGG 2 cut(s) 154, 336
TaaI ACNGT 2 cut(s) 220, 227
TaiI ACGT 1 cut(s) 20
TaqI TCGA 1 cut(s) 277
TasI AATT 2 cut(s) 86, 303
TatI WGTACW 1 cut(s) 183
TauI GCSGC 2 cut(s) 13, 123
TfiI GAWTC 2 cut(s) 150, 160
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TseI GCWGC 1 cut(s) 7
XbaI TCTAGA 1 cut(s) 194
XspI CTAG 3 cut(s) 195, 204, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.