FvH4_6g48700

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001880
Physical Location & Seq
Forward (+)
36639532 .. 36641263
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48700.t1

Sequence Viewer

Length: 363 bp
ATGGTGCAGCGGCTAACCTACCGTTCCCGCCACAGCTACGCCACCAAGTCCAACCAGCACCGCGTCGTCAAAACCCCCGGTGGGAAGTTGGTGTATCAGACTACTAAGAAGAGAGCCAGTGGACCAAAATGCCCTGTGACTGGCAAGAGAATCCAAGGAATTCCTCACTTGAGGCCTGCTGAGTACAAGAGGTCCAGATTGTCCAGGAACCGCAGGACTGTGAACCGTGCTTATGGTGGTGTCTTGTCTGGAGGTGCTGTCAGAGAGAGGATTATTCGAGCCTTCTTGGTAGAAGAGCAAAAGATTGTGAAGAAGGTTTTGAAGATCCAGAAGGCCAAGGAAAGGCAGGCAGCCAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L34e

Protein Analysis

121

Amino Acids

13.69

Weight (kDa)

11.67

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L34e PF01199 1 - 96 2.3e-38 Ribosomal protein L34e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 63
AciI CCGC 4 cut(s) 10, 28, 61, 211
AclWI GGATC 1 cut(s) 319
AcsI RAATTY 1 cut(s) 159
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 81, 140, 342
AgsI TTSAA 1 cut(s) 322
AjnI CCWGG 1 cut(s) 203
AluBI AGCT 2 cut(s) 36, 360
AluI AGCT 2 cut(s) 36, 360
AlwI GGATC 1 cut(s) 319
AoxI GGCC 2 cut(s) 173, 333
ApeKI GCWGC 2 cut(s) 7, 350
ApoI RAATTY 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 122, 192
AsuC2I CCSGG 1 cut(s) 78
AvaII GGWCC 2 cut(s) 122, 192
BbvI GCAGC 1 cut(s) 19
BciT130I CCWGG 1 cut(s) 205
BcnI CCSGG 1 cut(s) 78
BisI GCNGC 3 cut(s) 8, 11, 351
BlsI GCNGC 3 cut(s) 9, 12, 352
Bme1390I CCNGG 2 cut(s) 78, 205
Bme18I GGWCC 2 cut(s) 122, 192
BmgT120I GGNCC 2 cut(s) 122, 192
BmiI GGNNCC 1 cut(s) 209
BmrFI CCNGG 2 cut(s) 78, 205
BpmI CTGGAG 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 190
BpuMI CCSGG 1 cut(s) 78
BsaJI CCNNGG 3 cut(s) 76, 154, 336
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 3 cut(s) 81, 140, 342
Bse1I ACTGG 2 cut(s) 117, 145
BseBI CCWGG 1 cut(s) 205
BseDI CCNNGG 3 cut(s) 76, 154, 336
BseLI CCNNNNNNNGG 3 cut(s) 81, 140, 342
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 2 cut(s) 117, 145
BseXI GCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 175, 335
BsiSI CCGG 1 cut(s) 78
BslI CCNNNNNNNGG 3 cut(s) 81, 140, 342
BsnI GGCC 2 cut(s) 175, 335
Bsp143I GATC 1 cut(s) 324
BspACI CCGC 4 cut(s) 10, 28, 61, 211
BspANI GGCC 2 cut(s) 175, 335
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 209
BspPI GGATC 1 cut(s) 319
BspQI GCTCTTC 1 cut(s) 288
BsrI ACTGG 2 cut(s) 117, 145
BssECI CCNNGG 3 cut(s) 76, 154, 336
BssMI GATC 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 154, 336
Bst2UI CCWGG 1 cut(s) 205
Bst4CI ACNGT 3 cut(s) 23, 220, 227
Bst6I CTCTTC 2 cut(s) 104, 288
BstC8I GCNNGC 2 cut(s) 177, 348
BstDEI CTNAG 2 cut(s) 105, 180
BstFNI CGCG 1 cut(s) 63
BstKTI GATC 1 cut(s) 327
BstMBI GATC 1 cut(s) 324
BstNI CCWGG 1 cut(s) 205
BstSCI CCNGG 2 cut(s) 76, 203
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 19
BstX2I RGATCY 1 cut(s) 324
BstYI RGATCY 1 cut(s) 324
BsuRI GGCC 2 cut(s) 175, 335
BtsIMutI CAGTG 1 cut(s) 124
Cac8I GCNNGC 2 cut(s) 177, 348
Cfr13I GGNCC 2 cut(s) 122, 192
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 184
CviJI RGCY 8 cut(s) 13, 36, 116, 175, 281, 335, 353, 360
CviKI_1 RGCY 8 cut(s) 13, 36, 116, 175, 281, 335, 353, 360
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 2 cut(s) 105, 180
DpnI GATC 1 cut(s) 326
DpnII GATC 1 cut(s) 324
Eam1104I CTCTTC 2 cut(s) 104, 288
EarI CTCTTC 2 cut(s) 104, 288
Eco130I CCWWGG 2 cut(s) 154, 336
Eco147I AGGCCT 1 cut(s) 175
Eco47I GGWCC 2 cut(s) 122, 192
EcoRI GAATTC 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 203
EcoT14I CCWWGG 2 cut(s) 154, 336
ErhI CCWWGG 2 cut(s) 154, 336
FaiI YATR 1 cut(s) 234
FauI CCCGC 1 cut(s) 35
Fnu4HI GCNGC 3 cut(s) 8, 11, 351
Fsp4HI GCNGC 3 cut(s) 8, 11, 351
GluI GCNGC 3 cut(s) 8, 11, 351
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 2 cut(s) 175, 335
HapII CCGG 1 cut(s) 78
HgaI GACGC 1 cut(s) 52
HinfI GANTC 1 cut(s) 150
HpaII CCGG 1 cut(s) 78
Hpy166II GTNNAC 2 cut(s) 122, 223
Hpy188I TCNGA 2 cut(s) 99, 263
Hpy188III TCNNGA 3 cut(s) 195, 249, 328
Hpy8I GTNNAC 2 cut(s) 122, 223
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 292, 307, 325
HpyCH4III ACNGT 3 cut(s) 23, 220, 227
HpyCH4V TGCA 1 cut(s) 7
HpyF3I CTNAG 2 cut(s) 105, 180
Kzo9I GATC 1 cut(s) 324
LguI GCTCTTC 1 cut(s) 288
Lsp1109I GCAGC 1 cut(s) 19
MaeIII GTNAC 1 cut(s) 136
MalI GATC 1 cut(s) 326
MboI GATC 1 cut(s) 324
MboII GAAGA 4 cut(s) 121, 305, 322, 334
MflI RGATCY 1 cut(s) 324
MluCI AATT 1 cut(s) 159
MmeI TCCRAC 1 cut(s) 75
MnlI CCTC 5 cut(s) 165, 174, 183, 245, 261
MspA1I CMGCKG 1 cut(s) 10
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 2 cut(s) 78, 205
MvaI CCWGG 1 cut(s) 205
MvnI CGCG 1 cut(s) 63
NciI CCSGG 1 cut(s) 78
NdeII GATC 1 cut(s) 324
NlaIV GGNNCC 1 cut(s) 209
NmuCI GTSAC 1 cut(s) 136
PceI AGGCCT 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 288
PfeI GAWTC 1 cut(s) 150
PfoI TCCNGGA 1 cut(s) 203
PkrI GCNGC 3 cut(s) 9, 12, 352
Psp6I CCWGG 1 cut(s) 203
PspGI CCWGG 1 cut(s) 203
PspN4I GGNNCC 1 cut(s) 209
PspPI GGNCC 2 cut(s) 122, 192
PsuI RGATCY 1 cut(s) 324
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SapI GCTCTTC 1 cut(s) 288
SatI GCNGC 3 cut(s) 8, 11, 351
Sau3AI GATC 1 cut(s) 324
Sau96I GGNCC 2 cut(s) 122, 192
ScrFI CCNGG 2 cut(s) 78, 205
SetI ASST 6 cut(s) 20, 38, 194, 256, 318, 362
SinI GGWCC 2 cut(s) 122, 192
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 1 cut(s) 159
SseBI AGGCCT 1 cut(s) 175
SsiI CCGC 4 cut(s) 10, 28, 61, 211
StuI AGGCCT 1 cut(s) 175
StyD4I CCNGG 2 cut(s) 76, 203
StyI CCWWGG 2 cut(s) 154, 336
TaaI ACNGT 3 cut(s) 23, 220, 227
TaqI TCGA 1 cut(s) 277
TasI AATT 1 cut(s) 159
TatI WGTACW 1 cut(s) 183
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 1 cut(s) 150
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 2 cut(s) 7, 350
Tsp45I GTSAC 1 cut(s) 136
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 2 cut(s) 122, 192
XapI RAATTY 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.