FvH4_5g16990

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007511
Physical Location & Seq
Reverse (-)
9680181 .. 9681880
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16990.t1

Sequence Viewer

Length: 1023 bp
ATGGTGGGGATGCCAAATACAGCTACTATGTTTACTAATCAGAGAGAGGCAGCTGATCTATCAACTAGAACGCTGGTGGCAGCCATAGTCGAAAACCTTGACATTGATGCTCAGTCTCCTGCTACAAGCTCATTCCGGATTGCTGACTTAGGTTGTTCTACAGGGCCTAATACATTTTTCGCAATCAACAACATCATTGAAGCTGTAGCACAAAAATACCAGCTGGTTAAAGGCCACTCTGCTCATCTCCCAGAAATTCAAGTTTACTGTAGTGATCTTGTGTCAAATGATTTCAATCATCTCTTTGCCAATCTCCCTCAAGATAGGCGATACTTCGCGGCTGCAGTTCCGGGATCTTTTCATGGAAGGTTGTTTCCTAAAGCCTCCCTAGATTTTGTTTACTCTGAATATGCTCTCCAATGGCTTTCTAAGGTACCACCGGAGACACTTGACTCCAACTCACCTGCATTTAACAAGGGGAGGATTATGCATGAAAAGTCAGCTATTGAAGTTGTTCAGGCATTTTCGGATCAGTATGCCAAGGACATGCAGTGTTTCTTCGATGCTCGAGCACAAGAGCTTGCTCCTGGAGGGTTATTGTTGATGTTGATTCCAGGGCGTCCGGGGGCAACTCTTCCGGCACAACATCCCAGTTATCCGCGTTTTGAACCCTTGGAGTCCAGTCTATCCGATATGGTTAATGAGGGTTTAATAGGTGGAGAGAAATTTGACTCTTTCAACTTGCCTATCTACTCGCCATCCGTGGAAGAGCCAAGGAAGATAATAGAAAACAGTGAGTGCTTTGATGTGTTAAGGTTGGAGACTCAACCTGAGAGGCAAATCCCTCTTTGTCCTGAAGGAACTAGAGCGGGCTGTGAGAAAATTCTCACCGAACACTTCGGGAGTGAAATTATAGAACCACTTTTCGATCGATACTCGAAGAAAGTACAAGGTCGTTCTCCCATTAACTCTCGTGATGGTTATCTTGTCGGACTGTTCGTCCTTCTCAAACGCAGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

341

Amino Acids

37.82

Weight (kDa)

5.37

Isoelectric Point (pI)

54.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 32 - 338 7.9e-91 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 472
Acc65I GGTACC 1 cut(s) 433
AccB1I GGYRCC 1 cut(s) 433
AccBSI CCGCTC 1 cut(s) 869
AccII CGCG 2 cut(s) 338, 661
AccIII TCCGGA 1 cut(s) 135
AciI CCGC 3 cut(s) 338, 659, 869
AclWI GGATC 2 cut(s) 361, 537
AcsI RAATTY 3 cut(s) 255, 725, 882
AcuI CTGAAG 1 cut(s) 876
AcyI GRCGYC 1 cut(s) 619
AfaI GTAC 2 cut(s) 435, 948
AgsI TTSAA 6 cut(s) 200, 260, 295, 509, 668, 739
AhdI GACNNNNNGTC 1 cut(s) 998
AjnI CCWGG 2 cut(s) 586, 613
AluBI AGCT 7 cut(s) 23, 53, 129, 203, 223, 503, 580
AluI AGCT 7 cut(s) 23, 53, 129, 203, 223, 503, 580
Alw21I GWGCWC 1 cut(s) 574
Alw26I GTCTC 3 cut(s) 120, 437, 815
AlwI GGATC 2 cut(s) 361, 537
Ama87I CYCGRG 1 cut(s) 567
Aor13HI TCCGGA 1 cut(s) 135
AoxI GGCC 2 cut(s) 164, 232
ApeKI GCWGC 3 cut(s) 50, 80, 341
ApoI RAATTY 3 cut(s) 255, 725, 882
Asp700I GAANNNNTTC 1 cut(s) 513
Asp718I GGTACC 1 cut(s) 433
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 2 cut(s) 351, 624
AsuHPI GGTGA 2 cut(s) 453, 880
AvaI CYCGRG 1 cut(s) 567
BanI GGYRCC 1 cut(s) 433
BauI CACGAG 1 cut(s) 972
Bbv12I GWGCWC 1 cut(s) 574
BbvI GCAGC 3 cut(s) 62, 92, 328
BccI CCATC 2 cut(s) 766, 971
BciT130I CCWGG 2 cut(s) 588, 615
BcnI CCSGG 2 cut(s) 351, 624
BcoDI GTCTC 3 cut(s) 120, 437, 815
BfaI CTAG 3 cut(s) 66, 389, 864
BfmI CTRYAG 4 cut(s) 159, 204, 268, 342
BfuAI ACCTGC 1 cut(s) 472
BisI GCNGC 4 cut(s) 51, 81, 339, 342
BlsI GCNGC 4 cut(s) 52, 82, 340, 343
Bme1390I CCNGG 4 cut(s) 351, 588, 615, 624
BmeRI GACNNNNNGTC 1 cut(s) 998
BmeT110I CYCGRG 1 cut(s) 567
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 4 cut(s) 351, 588, 615, 624
BmrI ACTGGG 1 cut(s) 645
BmsI GCATC 2 cut(s) 97, 553
BmuI ACTGGG 1 cut(s) 645
BpmI CTGGAG 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 303
BpuMI CCSGG 2 cut(s) 351, 624
Bsa29I ATCGAT 1 cut(s) 931
BsaBI GATNNNNATC 2 cut(s) 294, 981
BsaHI GRCGYC 1 cut(s) 619
BsaJI CCNNGG 6 cut(s) 540, 614, 623, 672, 762, 773
BsaWI WCCGGW 2 cut(s) 135, 439
Bse1I ACTGG 2 cut(s) 651, 681
Bse8I GATNNNNATC 2 cut(s) 294, 981
BseAI TCCGGA 1 cut(s) 135
BseBI CCWGG 2 cut(s) 588, 615
BseCI ATCGAT 1 cut(s) 931
BseDI CCNNGG 6 cut(s) 540, 614, 623, 672, 762, 773
BseGI GGATG 3 cut(s) 15, 646, 758
BseJI GATNNNNATC 2 cut(s) 294, 981
BseMII CTCAG 2 cut(s) 125, 822
BseNI ACTGG 2 cut(s) 651, 681
BseXI GCAGC 3 cut(s) 62, 92, 328
Bsh1236I CGCG 2 cut(s) 338, 661
Bsh1285I CGRYCG 1 cut(s) 931
BshFI GGCC 2 cut(s) 166, 234
BshNI GGYRCC 1 cut(s) 433
BshVI ATCGAT 1 cut(s) 931
BsiEI CGRYCG 1 cut(s) 931
BsiHKAI GWGCWC 1 cut(s) 574
BsiHKCI CYCGRG 1 cut(s) 567
BsiSI CCGG 5 cut(s) 136, 350, 440, 623, 638
BsmAI GTCTC 3 cut(s) 120, 437, 815
BsnI GGCC 2 cut(s) 166, 234
BsoBI CYCGRG 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 574
Bsp13I TCCGGA 1 cut(s) 135
Bsp143I GATC 5 cut(s) 55, 274, 353, 529, 928
BspACI CCGC 3 cut(s) 338, 659, 869
BspANI GGCC 2 cut(s) 166, 234
BspCNI CTCAG 2 cut(s) 124, 823
BspDI ATCGAT 1 cut(s) 931
BspEI TCCGGA 1 cut(s) 135
BspFNI CGCG 2 cut(s) 338, 661
BspLI GGNNCC 1 cut(s) 435
BspMAI CTGCAG 1 cut(s) 346
BspMI ACCTGC 1 cut(s) 472
BspPI GGATC 2 cut(s) 361, 537
BspQI GCTCTTC 1 cut(s) 762
BspT107I GGYRCC 1 cut(s) 433
BsrBI CCGCTC 1 cut(s) 869
BsrI ACTGG 2 cut(s) 651, 681
BssECI CCNNGG 6 cut(s) 540, 614, 623, 672, 762, 773
BssMI GATC 5 cut(s) 55, 274, 353, 529, 928
BssNI GRCGYC 1 cut(s) 619
BssSI CACGAG 1 cut(s) 972
BssT1I CCWWGG 3 cut(s) 540, 672, 773
Bst2BI CACGAG 1 cut(s) 972
Bst2UI CCWGG 2 cut(s) 588, 615
Bst4CI ACNGT 3 cut(s) 269, 794, 996
Bst6I CTCTTC 2 cut(s) 639, 762
BstACI GRCGYC 1 cut(s) 619
BstC8I GCNNGC 2 cut(s) 582, 871
BstDEI CTNAG 4 cut(s) 111, 148, 429, 831
BstDSI CCRYGG 1 cut(s) 762
BstF5I GGATG 3 cut(s) 15, 646, 758
BstFNI CGCG 2 cut(s) 338, 661
BstKTI GATC 5 cut(s) 58, 277, 356, 532, 931
BstMAI GTCTC 3 cut(s) 120, 437, 815
BstMBI GATC 5 cut(s) 55, 274, 353, 529, 928
BstMCI CGRYCG 1 cut(s) 931
BstNI CCWGG 2 cut(s) 588, 615
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 4 cut(s) 349, 586, 613, 622
BstSFI CTRYAG 4 cut(s) 159, 204, 268, 342
BstUI CGCG 2 cut(s) 338, 661
BstV1I GCAGC 3 cut(s) 62, 92, 328
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
Bsu15I ATCGAT 1 cut(s) 931
BsuRI GGCC 2 cut(s) 166, 234
BsuTUI ATCGAT 1 cut(s) 931
BtgI CCRYGG 1 cut(s) 762
BtsCI GGATG 3 cut(s) 15, 646, 758
BtsI GCAGTG 1 cut(s) 557
BtsIMutI CAGTG 2 cut(s) 557, 799
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 2 cut(s) 582, 871
Cfr13I GGNCC 1 cut(s) 164
ClaI ATCGAT 1 cut(s) 931
CseI GACGC 1 cut(s) 608
Csp6I GTAC 2 cut(s) 434, 947
CviAII CATG 3 cut(s) 362, 491, 547
CviQI GTAC 2 cut(s) 434, 947
DdeI CTNAG 4 cut(s) 111, 148, 429, 831
DpnI GATC 5 cut(s) 57, 276, 355, 531, 930
DpnII GATC 5 cut(s) 55, 274, 353, 529, 928
DriI GACNNNNNGTC 1 cut(s) 998
Eam1104I CTCTTC 2 cut(s) 639, 762
Eam1105I GACNNNNNGTC 1 cut(s) 998
EarI CTCTTC 2 cut(s) 639, 762
Eco130I CCWWGG 3 cut(s) 540, 672, 773
Eco57I CTGAAG 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 567
EcoO109I RGGNCCY 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 586, 613
EcoT14I CCWWGG 3 cut(s) 540, 672, 773
EcoT22I ATGCAT 1 cut(s) 492
ErhI CCWWGG 3 cut(s) 540, 672, 773
FaeI CATG 3 cut(s) 365, 494, 550
FatI CATG 3 cut(s) 361, 490, 546
FauI CCCGC 1 cut(s) 862
Fnu4HI GCNGC 4 cut(s) 51, 81, 339, 342
FokI GGATG 3 cut(s) 22, 633, 745
Fsp4HI GCNGC 4 cut(s) 51, 81, 339, 342
FspBI CTAG 3 cut(s) 66, 389, 864
GluI GCNGC 4 cut(s) 51, 81, 339, 342
GsuI CTGGAG 1 cut(s) 609
HaeIII GGCC 2 cut(s) 166, 234
HapII CCGG 5 cut(s) 136, 350, 440, 623, 638
HgaI GACGC 1 cut(s) 608
Hin1I GRCGYC 1 cut(s) 619
Hin1II CATG 3 cut(s) 365, 494, 550
HinfI GANTC 5 cut(s) 452, 610, 677, 731, 823
HpaII CCGG 5 cut(s) 136, 350, 440, 623, 638
HphI GGTGA 2 cut(s) 453, 880
Hpy166II GTNNAC 3 cut(s) 33, 265, 400
Hpy188I TCNGA 5 cut(s) 42, 406, 529, 691, 992
Hpy188III TCNNGA 5 cut(s) 136, 320, 854, 901, 974
Hpy8I GTNNAC 3 cut(s) 33, 265, 400
HpyAV CCTTC 3 cut(s) 360, 851, 1013
HpyCH4III ACNGT 3 cut(s) 269, 794, 996
HpyCH4V TGCA 4 cut(s) 344, 467, 490, 550
HpyF3I CTNAG 4 cut(s) 111, 148, 429, 831
Hsp92I GRCGYC 1 cut(s) 619
Hsp92II CATG 3 cut(s) 365, 494, 550
Kpn2I TCCGGA 1 cut(s) 135
KpnI GGTACC 1 cut(s) 437
Kzo9I GATC 5 cut(s) 55, 274, 353, 529, 928
LguI GCTCTTC 1 cut(s) 762
LmnI GCTCC 1 cut(s) 589
Lsp1109I GCAGC 3 cut(s) 62, 92, 328
LweI GCATC 2 cut(s) 97, 553
MaeI CTAG 3 cut(s) 66, 389, 864
MalI GATC 5 cut(s) 57, 276, 355, 531, 930
MbiI CCGCTC 1 cut(s) 869
MboI GATC 5 cut(s) 55, 274, 353, 529, 928
MboII GAAGA 5 cut(s) 550, 626, 779, 790, 952
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 574
MluCI AATT 4 cut(s) 255, 725, 882, 909
MlyI GAGTC 4 cut(s) 446, 686, 725, 817
MmeI TCCRAC 3 cut(s) 480, 798, 970
MnlI CCTC 8 cut(s) 40, 327, 394, 474, 584, 697, 828, 855
Mph1103I ATGCAT 1 cut(s) 492
MroI TCCGGA 1 cut(s) 135
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 6 cut(s) 228, 471, 699, 710, 812, 966
MspA1I CMGCKG 2 cut(s) 53, 223
MspI CCGG 5 cut(s) 136, 350, 440, 623, 638
MspR9I CCNGG 4 cut(s) 351, 588, 615, 624
MvaI CCWGG 2 cut(s) 588, 615
MvnI CGCG 2 cut(s) 338, 661
NciI CCSGG 2 cut(s) 351, 624
NdeII GATC 5 cut(s) 55, 274, 353, 529, 928
NlaIII CATG 3 cut(s) 365, 494, 550
NlaIV GGNNCC 1 cut(s) 435
NsiI ATGCAT 1 cut(s) 492
NspI RCATGY 1 cut(s) 550
PaeR7I CTCGAG 1 cut(s) 567
PaqCI CACCTGC 1 cut(s) 472
PciSI GCTCTTC 1 cut(s) 762
PcsI WCGNNNNNNNCGW 1 cut(s) 996
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 610
PfoI TCCNGGA 2 cut(s) 349, 586
PkrI GCNGC 4 cut(s) 52, 82, 340, 343
Ple19I CGATCG 1 cut(s) 931
PleI GAGTC 4 cut(s) 446, 685, 725, 817
PpsI GAGTC 4 cut(s) 446, 685, 725, 817
Psp6I CCWGG 2 cut(s) 586, 613
PspGI CCWGG 2 cut(s) 586, 613
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 1 cut(s) 164
PspXI VCTCGAGB 1 cut(s) 567
PstI CTGCAG 1 cut(s) 346
PsuI RGATCY 1 cut(s) 353
PvuI CGATCG 1 cut(s) 931
PvuII CAGCTG 2 cut(s) 53, 223
RsaI GTAC 2 cut(s) 435, 948
RsaNI GTAC 2 cut(s) 434, 947
SapI GCTCTTC 1 cut(s) 762
SaqAI TTAA 6 cut(s) 228, 471, 699, 710, 812, 966
SatI GCNGC 4 cut(s) 51, 81, 339, 342
Sau3AI GATC 5 cut(s) 55, 274, 353, 529, 928
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 4 cut(s) 446, 686, 725, 817
ScrFI CCNGG 4 cut(s) 351, 588, 615, 624
SduI GDGCHC 1 cut(s) 574
SfaNI GCATC 2 cut(s) 97, 553
SfcI CTRYAG 4 cut(s) 159, 204, 268, 342
Sfr274I CTCGAG 1 cut(s) 567
SlaI CTCGAG 1 cut(s) 567
SmlI CTYRAG 2 cut(s) 318, 567
SmoI CTYRAG 2 cut(s) 318, 567
Sse9I AATT 4 cut(s) 255, 725, 882, 909
SsiI CCGC 3 cut(s) 338, 659, 869
SspMI CTAG 3 cut(s) 66, 389, 864
StyD4I CCNGG 4 cut(s) 349, 586, 613, 622
StyI CCWWGG 3 cut(s) 540, 672, 773
TaaI ACNGT 3 cut(s) 269, 794, 996
TaqI TCGA 6 cut(s) 90, 561, 568, 927, 931, 938
TasI AATT 4 cut(s) 255, 725, 882, 909
TatI WGTACW 1 cut(s) 946
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 1 cut(s) 610
Tru1I TTAA 6 cut(s) 228, 471, 699, 710, 812, 966
Tru9I TTAA 6 cut(s) 228, 471, 699, 710, 812, 966
TscAI CASTG 2 cut(s) 557, 799
TseI GCWGC 3 cut(s) 50, 80, 341
TspDTI ATGAA 2 cut(s) 350, 507
TspGWI ACGGA 1 cut(s) 751
TspRI CASTG 2 cut(s) 557, 799
XapI RAATTY 3 cut(s) 255, 725, 882
XceI RCATGY 1 cut(s) 550
XhoI CTCGAG 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 3 cut(s) 66, 389, 864
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.