Rh7CG020000

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0007511
Physical Location & Seq
Forward (+)
1501646 .. 1503030
1385 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG020000.1

Sequence Viewer

Length: 1056 bp
ATGGCCAGCTCTGCACATACAATGAATGGTGGGGATGGAAAGTACAGCTACTCTGTTCACTCTTATGATCATAGAGAGGCAGCTGATCTATCAAAGAGAATGCTGGTGGAAGCCATAGTCGAAAACCTTGACATTGAAACTCAGTCTCCTACTACAAGCTCATTCCGGATTGCTGACTTAGGTTGTTCTGTTGGGCCTAATACATTTTTCGCAATCAACAACATCATTGAAGCTGTAGCACAAAAATACCAGCTTATTAAAGGCCACTCTGATCTCCCAGCAGAAATTCAAGTTTACTTTAGTGATTTGGTGTCCAATGATTTCAATCATCTCTTTGCCAGTCTCCCGCAAGATAGGCAATATTTTGCGGCTGGAGTTCCGGGATCTTTTCATGGAAGATTGTTTCCTAAAGCCTCCCTCGATTTTGTCTACTCTCAGTATGCTCTCCAATGGCTTTCTAGGGTACCACCGGAGATACTTGACTCCAACTCACCTGCATTCAACAAGGGGAGGATTTTGTATGGAAATTCGGCTAGAGAAGTTGGTCAGGCATTTTCGGATCAGTATGCCAAGGACATGCAGTCTTTTTTTGATGCTCGAGCCCATGAGCTTGCTCCTGGAGGGTTATTGTTGCTCTTGATTCCAGGGCGTCCGGATGGAACTGTTCCGGCACAACATTCCGTACATCCGCTTTTTGAACCCATGGAGTCTAGTCTATCCGATATGGTTAATCAGGGTTTAATAAGTGGAGATAAACTTGACTCTTTCAACTTGCCTATCTACTCTCCATCCATGGAAGAGCTAAGGAAGATAATAGAAAACAGCGGGTGCTTCGATGTATTGAGGTTGGAGATCCAATTACCTGAGAGGCCAACCCCTTTGCGTAGTGAGAGTGCTAGATCAGGCTTTGAGAAAATTCTCACCAAACACTTTGGGACTGAAATTATAGAACCACTTTTCAATCGATACTCCAAGAAAGTACAAGGTCGTTCTCCCATTACTTCTTGTGATGGTATGGCTGTTGGATTGTTCGTCCTTCTCAAGCGCAATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

351

Amino Acids

38.93

Weight (kDa)

5.7

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 41 - 349 6e-96 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 502
Acc36I ACCTGC 1 cut(s) 502
Acc65I GGTACC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 429
AccIII TCCGGA 2 cut(s) 165, 652
AciI CCGC 4 cut(s) 347, 368, 689, 825
AclWI GGATC 3 cut(s) 391, 567, 847
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 285, 526, 915
AcyI GRCGYC 1 cut(s) 649
AfaI GTAC 4 cut(s) 44, 465, 684, 981
AgsI TTSAA 8 cut(s) 137, 230, 290, 325, 502, 698, 769, 961
AhdI GACNNNNNGTC 1 cut(s) 580
AjnI CCWGG 2 cut(s) 616, 643
AluBI AGCT 8 cut(s) 9, 48, 83, 159, 233, 253, 610, 802
AluI AGCT 8 cut(s) 9, 48, 83, 159, 233, 253, 610, 802
Alw26I GTCTC 2 cut(s) 150, 347
AlwI GGATC 3 cut(s) 391, 567, 847
Ama87I CYCGRG 1 cut(s) 597
Aor13HI TCCGGA 2 cut(s) 165, 652
AoxI GGCC 4 cut(s) 3, 194, 262, 869
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 3 cut(s) 285, 526, 915
Asp718I GGTACC 1 cut(s) 463
AspLEI GCGC 1 cut(s) 1047
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 2 cut(s) 483, 913
AvaI CYCGRG 1 cut(s) 597
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 604
BbvI GCAGC 1 cut(s) 92
BccI CCATC 4 cut(s) 29, 650, 796, 1004
BciT130I CCWGG 2 cut(s) 618, 645
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 150, 347
BfaI CTAG 4 cut(s) 459, 534, 711, 897
BfmI CTRYAG 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 502
BisI GCNGC 2 cut(s) 81, 369
BlsI GCNGC 2 cut(s) 82, 370
Bme1390I CCNGG 3 cut(s) 381, 618, 645
BmeRI GACNNNNNGTC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 597
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 3 cut(s) 381, 618, 645
BmsI GCATC 1 cut(s) 583
BpmI CTGGAG 2 cut(s) 393, 639
Bpu10I CCTNAGC 1 cut(s) 803
BpuEI CTTGAG 1 cut(s) 1025
BpuMI CCSGG 1 cut(s) 381
Bsa29I ATCGAT 1 cut(s) 964
BsaBI GATNNNNATC 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 649
BsaJI CCNNGG 4 cut(s) 570, 644, 702, 792
BsaWI WCCGGW 3 cut(s) 165, 469, 652
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bse1I ACTGG 1 cut(s) 339
Bse8I GATNNNNATC 1 cut(s) 324
BseAI TCCGGA 2 cut(s) 165, 652
BseBI CCWGG 2 cut(s) 618, 645
BseCI ATCGAT 1 cut(s) 964
BseDI CCNNGG 4 cut(s) 570, 644, 702, 792
BseGI GGATG 4 cut(s) 40, 661, 685, 788
BseJI GATNNNNATC 1 cut(s) 324
BseMII CTCAG 3 cut(s) 155, 449, 855
BseNI ACTGG 1 cut(s) 339
BseXI GCAGC 1 cut(s) 92
BseYI CCCAGC 1 cut(s) 277
BshFI GGCC 4 cut(s) 5, 196, 264, 871
BshNI GGYRCC 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 964
BsiHKCI CYCGRG 1 cut(s) 597
BsiSI CCGG 5 cut(s) 166, 380, 470, 653, 668
BslFI GGGAC 1 cut(s) 949
BsmAI GTCTC 2 cut(s) 150, 347
BsmFI GGGAC 1 cut(s) 949
BsmI GAATGC 2 cut(s) 105, 497
BsnI GGCC 4 cut(s) 5, 196, 264, 871
BsoBI CYCGRG 1 cut(s) 597
Bsp1286I GDGCHC 1 cut(s) 604
Bsp13I TCCGGA 2 cut(s) 165, 652
Bsp143I GATC 7 cut(s) 67, 85, 271, 383, 559, 852, 899
Bsp19I CCATGG 2 cut(s) 702, 792
BspACI CCGC 4 cut(s) 347, 368, 689, 825
BspANI GGCC 4 cut(s) 5, 196, 264, 871
BspCNI CTCAG 3 cut(s) 154, 448, 856
BspDI ATCGAT 1 cut(s) 964
BspEI TCCGGA 2 cut(s) 165, 652
BspLI GGNNCC 1 cut(s) 465
BspMI ACCTGC 1 cut(s) 502
BspPI GGATC 3 cut(s) 391, 567, 847
BspQI GCTCTTC 1 cut(s) 792
BspT107I GGYRCC 1 cut(s) 463
BsrI ACTGG 1 cut(s) 339
BssECI CCNNGG 4 cut(s) 570, 644, 702, 792
BssMI GATC 7 cut(s) 67, 85, 271, 383, 559, 852, 899
BssNI GRCGYC 1 cut(s) 649
BssT1I CCWWGG 3 cut(s) 570, 702, 792
Bst2UI CCWGG 2 cut(s) 618, 645
Bst4CI ACNGT 1 cut(s) 664
Bst6I CTCTTC 1 cut(s) 792
BstACI GRCGYC 1 cut(s) 649
BstC8I GCNNGC 2 cut(s) 7, 612
BstDEI CTNAG 5 cut(s) 141, 178, 435, 803, 864
BstDSI CCRYGG 2 cut(s) 702, 792
BstF5I GGATG 4 cut(s) 40, 661, 685, 788
BstHHI GCGC 1 cut(s) 1047
BstKTI GATC 7 cut(s) 70, 88, 274, 386, 562, 855, 902
BstMAI GTCTC 2 cut(s) 150, 347
BstMBI GATC 7 cut(s) 67, 85, 271, 383, 559, 852, 899
BstMWI GCNNNNNNNGC 2 cut(s) 11, 355
BstNI CCWGG 2 cut(s) 618, 645
BstNSI RCATGY 1 cut(s) 580
BstSCI CCNGG 3 cut(s) 379, 616, 643
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 92
BstX2I RGATCY 2 cut(s) 383, 852
BstYI RGATCY 2 cut(s) 383, 852
Bsu15I ATCGAT 1 cut(s) 964
BsuRI GGCC 4 cut(s) 5, 196, 264, 871
BsuTUI ATCGAT 1 cut(s) 964
BtgI CCRYGG 2 cut(s) 702, 792
BtsCI GGATG 4 cut(s) 40, 661, 685, 788
BveI ACCTGC 1 cut(s) 502
Cac8I GCNNGC 2 cut(s) 7, 612
CfoI GCGC 1 cut(s) 1047
Cfr13I GGNCC 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 964
CseI GACGC 1 cut(s) 638
Csp6I GTAC 4 cut(s) 43, 464, 683, 980
CviAII CATG 5 cut(s) 392, 577, 605, 703, 793
CviQI GTAC 4 cut(s) 43, 464, 683, 980
DdeI CTNAG 5 cut(s) 141, 178, 435, 803, 864
DpnI GATC 7 cut(s) 69, 87, 273, 385, 561, 854, 901
DpnII GATC 7 cut(s) 67, 85, 271, 383, 559, 852, 899
DriI GACNNNNNGTC 1 cut(s) 580
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 792
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 792
Eco130I CCWWGG 3 cut(s) 570, 702, 792
Eco24I GRGCYC 1 cut(s) 604
Eco88I CYCGRG 1 cut(s) 597
EcoRII CCWGG 2 cut(s) 616, 643
EcoT14I CCWWGG 3 cut(s) 570, 702, 792
EcoT38I GRGCYC 1 cut(s) 604
ErhI CCWWGG 3 cut(s) 570, 702, 792
FaeI CATG 5 cut(s) 395, 580, 608, 706, 796
FaqI GGGAC 1 cut(s) 949
FatI CATG 5 cut(s) 391, 576, 604, 702, 792
FauI CCCGC 2 cut(s) 354, 818
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 429
Fnu4HI GCNGC 2 cut(s) 81, 369
FokI GGATG 4 cut(s) 47, 668, 672, 775
FriOI GRGCYC 1 cut(s) 604
Fsp4HI GCNGC 2 cut(s) 81, 369
FspBI CTAG 4 cut(s) 459, 534, 711, 897
GlaI GCGC 1 cut(s) 1046
GluI GCNGC 2 cut(s) 81, 369
GsaI CCCAGC 1 cut(s) 281
GsuI CTGGAG 2 cut(s) 393, 639
HaeIII GGCC 4 cut(s) 5, 196, 264, 871
HapII CCGG 5 cut(s) 166, 380, 470, 653, 668
HgaI GACGC 1 cut(s) 638
HhaI GCGC 1 cut(s) 1047
Hin1I GRCGYC 1 cut(s) 649
Hin1II CATG 5 cut(s) 395, 580, 608, 706, 796
Hin6I GCGC 1 cut(s) 1045
HinP1I GCGC 1 cut(s) 1045
HinfI GANTC 4 cut(s) 482, 640, 707, 761
HpaII CCGG 5 cut(s) 166, 380, 470, 653, 668
HphI GGTGA 2 cut(s) 483, 913
Hpy166II GTNNAC 3 cut(s) 58, 295, 430
Hpy188I TCNGA 3 cut(s) 271, 559, 721
Hpy188III TCNNGA 3 cut(s) 166, 637, 653
Hpy8I GTNNAC 3 cut(s) 58, 295, 430
HpyAV CCTTC 1 cut(s) 1046
HpyCH4III ACNGT 1 cut(s) 664
HpyCH4V TGCA 3 cut(s) 14, 497, 580
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 355
HpyF3I CTNAG 5 cut(s) 141, 178, 435, 803, 864
Hsp92I GRCGYC 1 cut(s) 649
Hsp92II CATG 5 cut(s) 395, 580, 608, 706, 796
HspAI GCGC 1 cut(s) 1045
Kpn2I TCCGGA 2 cut(s) 165, 652
KpnI GGTACC 1 cut(s) 467
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 7 cut(s) 67, 85, 271, 383, 559, 852, 899
LguI GCTCTTC 1 cut(s) 792
LmnI GCTCC 1 cut(s) 619
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 1 cut(s) 583
MaeI CTAG 4 cut(s) 459, 534, 711, 897
MalI GATC 7 cut(s) 69, 87, 273, 385, 561, 854, 901
MboI GATC 7 cut(s) 67, 85, 271, 383, 559, 852, 899
MboII GAAGA 3 cut(s) 408, 809, 820
MflI RGATCY 2 cut(s) 383, 852
MhlI GDGCHC 1 cut(s) 604
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 285, 526, 857, 915, 942
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 476, 716, 755
MmeI TCCRAC 3 cut(s) 510, 828, 1003
MnlI CCTC 7 cut(s) 70, 424, 428, 504, 614, 837, 861
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 2 cut(s) 165, 652
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 258, 729, 740, 1054
MslI CAYNNNNRTG 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 83, 825
MspI CCGG 5 cut(s) 166, 380, 470, 653, 668
MspR9I CCNGG 3 cut(s) 381, 618, 645
Mva1269I GAATGC 2 cut(s) 105, 497
MvaI CCWGG 2 cut(s) 618, 645
MwoI GCNNNNNNNGC 2 cut(s) 11, 355
NciI CCSGG 1 cut(s) 381
NcoI CCATGG 2 cut(s) 702, 792
NdeII GATC 7 cut(s) 67, 85, 271, 383, 559, 852, 899
NlaIII CATG 5 cut(s) 395, 580, 608, 706, 796
NlaIV GGNNCC 1 cut(s) 465
NspI RCATGY 1 cut(s) 580
PaeR7I CTCGAG 1 cut(s) 597
PaqCI CACCTGC 1 cut(s) 502
PciSI GCTCTTC 1 cut(s) 792
PctI GAATGC 2 cut(s) 105, 497
PfeI GAWTC 1 cut(s) 640
PfoI TCCNGGA 2 cut(s) 379, 616
PkrI GCNGC 2 cut(s) 82, 370
PleI GAGTC 3 cut(s) 476, 715, 755
PpsI GAGTC 3 cut(s) 476, 715, 755
Psp6I CCWGG 2 cut(s) 616, 643
PspFI CCCAGC 1 cut(s) 277
PspGI CCWGG 2 cut(s) 616, 643
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 194
PspXI VCTCGAGB 1 cut(s) 597
PsuI RGATCY 2 cut(s) 383, 852
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 4 cut(s) 44, 465, 684, 981
RsaNI GTAC 4 cut(s) 43, 464, 683, 980
RseI CAYNNNNRTG 1 cut(s) 63
SapI GCTCTTC 1 cut(s) 792
SaqAI TTAA 4 cut(s) 258, 729, 740, 1054
SatI GCNGC 2 cut(s) 81, 369
Sau3AI GATC 7 cut(s) 67, 85, 271, 383, 559, 852, 899
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 3 cut(s) 476, 716, 755
ScrFI CCNGG 3 cut(s) 381, 618, 645
SduI GDGCHC 1 cut(s) 604
SfaNI GCATC 1 cut(s) 583
SfcI CTRYAG 1 cut(s) 234
Sfr274I CTCGAG 1 cut(s) 597
SlaI CTCGAG 1 cut(s) 597
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 2 cut(s) 597, 1040
SmoI CTYRAG 2 cut(s) 597, 1040
Sse9I AATT 5 cut(s) 285, 526, 857, 915, 942
SsiI CCGC 4 cut(s) 347, 368, 689, 825
SspI AATATT 1 cut(s) 362
SspMI CTAG 4 cut(s) 459, 534, 711, 897
StyD4I CCNGG 3 cut(s) 379, 616, 643
StyI CCWWGG 3 cut(s) 570, 702, 792
TaaI ACNGT 1 cut(s) 664
TaqI TCGA 5 cut(s) 120, 420, 598, 834, 964
TasI AATT 5 cut(s) 285, 526, 857, 915, 942
TatI WGTACW 2 cut(s) 42, 979
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 640
Tru1I TTAA 4 cut(s) 258, 729, 740, 1054
Tru9I TTAA 4 cut(s) 258, 729, 740, 1054
TseI GCWGC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 38, 380
TspGWI ACGGA 1 cut(s) 670
XapI RAATTY 3 cut(s) 285, 526, 915
XceI RCATGY 1 cut(s) 580
XhoI CTCGAG 1 cut(s) 597
XmiI GTMKAC 1 cut(s) 429
XspI CTAG 4 cut(s) 459, 534, 711, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.