MD06G1224100.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007511
Physical Location & Seq
Reverse (-)
35465365 .. 35468926
3562 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1224100.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGAAGATGAAATATTTCCTATGATGGGAGGAGATGGCCCAAACAGCTACGCCAAAAATTCTAATATACAGGTATGCCTCCGGGCAGTCTCCGGCGAATCGAGTCATGCTAGAAAGGCAGCTGATGTTATAACGTCCGGAAGATTGGTTCCAGCAATATTCGAAAACCTCGACATAGAAAATCCGCAGCAGTCTTCCATTTCAACTTCATTCCGGATTGCCGATTTGGGGTGTTCGGTTGGACCCAACACATTCATTCAAGTCCAGAACATAGTTGATGCAGTAGCTCAAAAATATCAGCACACAAGAATTCAAGTACCAGAATTTCATTTATACTTCAGTGACCTTGTTTCGAATGATTTCAACTATCTTTTCGCCACTCTCCCCCGAGACAGGCAGTATTTTGTGGCGGGTGTCCCGGGCTCTTTCCATGGAAGGCTCTTCCCTAAAGCCTCCCTTAATTTTGTCTACTCGGCGTATGCTCTTCATTGGCTTTCCAAGCTTCCTCAAGAACTACGTGACCCGAAATCGCCTGCATTTAACAAGAAGAAGATTTCATATGGCAGTGCGCCTTTTGAAGTTGGCCATGCATATTCAGTTCAATATGCCAAGGACATTGAGGCATTTTTTAGCGCTCGAGAGCAAGAGCTTGTCCCCGGAGGATTGATGGTGCTTCTGATTCCGGGGCGTCCCAATTCTACGCTTCCGGCGGAGAGTTATCCTGCCCCGGTTTTTGAATCTTTGGAGTCTACTCTTTCTGACATGGCGAATGAGGGGTTAATATGTGAAGATAAATTTGAGTCATTCAACTTGCCCATCTACTGCCCATCCGTGGAGGAGCTGCGAACCCTAATAGAGGAAAATGGTTGCTTTGATATACTGAAGCTGGAGCTCCAACCTCCGATGTCTATCAGCTTGCCTAGCGTGCAAGAATGTAGAGCTGGGATGGAGACCCTTCTCCAAAAACACTTCGGGGACGAAGCTAATATAGAACCGCTTTTTGATCGGTACTCGAAAAAAACTACTGCTGGAAATGTTGCTGAAGATGGCATGGCTGTTGGATTCTTCGTCCTTCTCAAGCGCAGATATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

365

Amino Acids

40.55

Weight (kDa)

5.14

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 65 - 361 5.5e-94 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AccI GTMKAC 2 cut(s) 468, 749
AccIII TCCGGA 2 cut(s) 137, 213
AciI CCGC 4 cut(s) 185, 410, 710, 995
AcoI YGGCCR 1 cut(s) 583
AcsI RAATTY 4 cut(s) 58, 309, 323, 794
AcuI CTGAAG 3 cut(s) 322, 902, 1062
AcyI GRCGYC 1 cut(s) 688
AfaI GTAC 2 cut(s) 318, 1010
AfeI AGCGCT 1 cut(s) 634
AfiI CCNNNNNNNGG 5 cut(s) 26, 228, 393, 832, 856
AgsI TTSAA 8 cut(s) 204, 260, 314, 364, 578, 602, 737, 808
AloI GAACNNNNNNTCC 2 cut(s) 132, 164
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 3 cut(s) 94, 384, 944
Ama87I CYCGRG 3 cut(s) 387, 418, 636
Aor13HI TCCGGA 2 cut(s) 137, 213
Aor51HI AGCGCT 1 cut(s) 634
AoxI GGCC 2 cut(s) 37, 583
ApeKI GCWGC 3 cut(s) 119, 187, 841
ApoI RAATTY 4 cut(s) 58, 309, 323, 794
Asp700I GAANNNNTTC 2 cut(s) 15, 359
AspLEI GCGC 3 cut(s) 571, 635, 1083
AspS9I GGNCC 2 cut(s) 38, 242
AsuC2I CCSGG 6 cut(s) 83, 419, 420, 657, 684, 728
AsuII TTCGAA 2 cut(s) 162, 353
AvaI CYCGRG 3 cut(s) 387, 418, 636
AvaII GGWCC 1 cut(s) 242
BalI TGGCCA 1 cut(s) 585
BanII GRGCYC 2 cut(s) 425, 894
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 894
BbvI GCAGC 3 cut(s) 131, 199, 828
BccI CCATC 7 cut(s) 19, 29, 661, 824, 835, 940, 1040
BcnI CCSGG 6 cut(s) 83, 419, 420, 657, 684, 728
BcoDI GTCTC 3 cut(s) 94, 384, 944
BfaI CTAG 2 cut(s) 111, 921
BfoI RGCGCY 1 cut(s) 636
BisI GCNGC 3 cut(s) 120, 188, 842
BlsI GCNGC 3 cut(s) 121, 189, 843
Bme1390I CCNGG 6 cut(s) 83, 419, 420, 657, 684, 728
Bme18I GGWCC 1 cut(s) 242
BmeT110I CYCGRG 3 cut(s) 387, 418, 636
BmgT120I GGNCC 2 cut(s) 38, 242
BmiI GGNNCC 2 cut(s) 150, 244
BmrFI CCNGG 6 cut(s) 83, 419, 420, 657, 684, 728
BmsI GCATC 1 cut(s) 268
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 908
Bpu14I TTCGAA 2 cut(s) 162, 353
BpuEI CTTGAG 2 cut(s) 492, 1061
BpuMI CCSGG 6 cut(s) 83, 419, 420, 657, 684, 728
BsaAI YACGTR 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 908
BsaHI GRCGYC 1 cut(s) 688
BsaI GGTCTC 1 cut(s) 944
BsaJI CCNNGG 7 cut(s) 418, 430, 609, 655, 683, 726, 831
BsaWI WCCGGW 2 cut(s) 137, 213
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 228, 393, 832, 856
Bse8I GATNNNNATC 1 cut(s) 908
BseAI TCCGGA 2 cut(s) 137, 213
BseDI CCNNGG 7 cut(s) 418, 430, 609, 655, 683, 726, 831
BseGI GGATG 2 cut(s) 827, 951
BseJI GATNNNNATC 1 cut(s) 908
BseLI CCNNNNNNNGG 5 cut(s) 26, 228, 393, 832, 856
BseRI GAGGAG 2 cut(s) 45, 851
BseXI GCAGC 3 cut(s) 131, 199, 828
BseYI CCCAGC 1 cut(s) 941
BshFI GGCC 2 cut(s) 39, 585
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 3 cut(s) 387, 418, 636
BsiSI CCGG 9 cut(s) 82, 93, 138, 214, 419, 657, 683, 707, 728
BslFI GGGAC 4 cut(s) 401, 638, 675, 989
BslI CCNNNNNNNGG 5 cut(s) 26, 228, 393, 832, 856
BsmAI GTCTC 3 cut(s) 94, 384, 944
BsmFI GGGAC 4 cut(s) 401, 638, 675, 989
BsnI GGCC 2 cut(s) 39, 585
Bso31I GGTCTC 1 cut(s) 944
BsoBI CYCGRG 3 cut(s) 387, 418, 636
Bsp119I TTCGAA 2 cut(s) 162, 353
Bsp1286I GDGCHC 2 cut(s) 425, 894
Bsp13I TCCGGA 2 cut(s) 137, 213
Bsp143I GATC 1 cut(s) 1003
Bsp19I CCATGG 1 cut(s) 430
BspACI CCGC 4 cut(s) 185, 410, 710, 995
BspANI GGCC 2 cut(s) 39, 585
BspEI TCCGGA 2 cut(s) 137, 213
BspLI GGNNCC 2 cut(s) 150, 244
BspQI GCTCTTC 2 cut(s) 446, 489
BspT104I TTCGAA 2 cut(s) 162, 353
BspTNI GGTCTC 1 cut(s) 944
BssECI CCNNGG 7 cut(s) 418, 430, 609, 655, 683, 726, 831
BssMI GATC 1 cut(s) 1003
BssNI GRCGYC 1 cut(s) 688
BssT1I CCWWGG 2 cut(s) 430, 609
Bst6I CTCTTC 2 cut(s) 446, 489
BstACI GRCGYC 1 cut(s) 688
BstBAI YACGTR 1 cut(s) 518
BstBI TTCGAA 2 cut(s) 162, 353
BstC8I GCNNGC 3 cut(s) 534, 917, 926
BstDSI CCRYGG 2 cut(s) 430, 831
BstENI CCTNNNNNAGG 1 cut(s) 854
BstF5I GGATG 2 cut(s) 827, 951
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 3 cut(s) 571, 635, 1083
BstKTI GATC 1 cut(s) 1006
BstMAI GTCTC 3 cut(s) 94, 384, 944
BstMBI GATC 1 cut(s) 1003
BstMWI GCNNNNNNNGC 5 cut(s) 45, 116, 499, 921, 925
BstSCI CCNGG 6 cut(s) 81, 417, 418, 655, 682, 726
BstV1I GCAGC 3 cut(s) 131, 199, 828
BstV2I GAAGAC 1 cut(s) 186
BsuRI GGCC 2 cut(s) 39, 585
BtgI CCRYGG 2 cut(s) 430, 831
BtsCI GGATG 2 cut(s) 827, 951
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 2 cut(s) 346, 571
Cac8I GCNNGC 3 cut(s) 534, 917, 926
CfoI GCGC 3 cut(s) 571, 635, 1083
Cfr13I GGNCC 2 cut(s) 38, 242
Cfr9I CCCGGG 1 cut(s) 418
CseI GACGC 1 cut(s) 677
Csp6I GTAC 2 cut(s) 317, 1009
CviAII CATG 5 cut(s) 107, 431, 587, 763, 1051
CviQI GTAC 2 cut(s) 317, 1009
DpnI GATC 1 cut(s) 1005
DpnII GATC 1 cut(s) 1003
EaeI YGGCCR 1 cut(s) 583
Eam1104I CTCTTC 2 cut(s) 446, 489
EarI CTCTTC 2 cut(s) 446, 489
EciI GGCGGA 1 cut(s) 725
Ecl136II GAGCTC 1 cut(s) 892
Eco130I CCWWGG 2 cut(s) 430, 609
Eco24I GRGCYC 2 cut(s) 425, 894
Eco31I GGTCTC 1 cut(s) 944
Eco32I GATATC 1 cut(s) 1088
Eco47I GGWCC 1 cut(s) 242
Eco47III AGCGCT 1 cut(s) 634
Eco53kI GAGCTC 1 cut(s) 892
Eco57I CTGAAG 3 cut(s) 322, 902, 1062
Eco88I CYCGRG 3 cut(s) 387, 418, 636
EcoICRI GAGCTC 1 cut(s) 892
EcoNI CCTNNNNNAGG 1 cut(s) 854
EcoRI GAATTC 1 cut(s) 309
EcoRV GATATC 1 cut(s) 1088
EcoT14I CCWWGG 2 cut(s) 430, 609
EcoT22I ATGCAT 1 cut(s) 592
EcoT38I GRGCYC 2 cut(s) 425, 894
ErhI CCWWGG 2 cut(s) 430, 609
FaeI CATG 5 cut(s) 110, 434, 590, 766, 1054
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 4 cut(s) 401, 638, 675, 989
FatI CATG 5 cut(s) 106, 430, 586, 762, 1050
FauI CCCGC 1 cut(s) 403
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 2 cut(s) 468, 749
Fnu4HI GCNGC 3 cut(s) 120, 188, 842
FokI GGATG 2 cut(s) 814, 958
FriOI GRGCYC 2 cut(s) 425, 894
Fsp4HI GCNGC 3 cut(s) 120, 188, 842
FspBI CTAG 2 cut(s) 111, 921
GlaI GCGC 3 cut(s) 570, 634, 1082
GluI GCNGC 3 cut(s) 120, 188, 842
GsaI CCCAGC 1 cut(s) 945
GsuI CTGGAG 1 cut(s) 908
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 2 cut(s) 39, 585
HapII CCGG 9 cut(s) 82, 93, 138, 214, 419, 657, 683, 707, 728
HgaI GACGC 1 cut(s) 677
HhaI GCGC 3 cut(s) 571, 635, 1083
Hin1I GRCGYC 1 cut(s) 688
Hin1II CATG 5 cut(s) 110, 434, 590, 766, 1054
Hin6I GCGC 3 cut(s) 569, 633, 1081
HinP1I GCGC 3 cut(s) 569, 633, 1081
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 7 cut(s) 98, 103, 679, 737, 746, 800, 1062
HpaII CCGG 9 cut(s) 82, 93, 138, 214, 419, 657, 683, 707, 728
Hpy166II GTNNAC 2 cut(s) 469, 750
Hpy188I TCNGA 3 cut(s) 678, 760, 903
Hpy188III TCNNGA 5 cut(s) 138, 214, 265, 509, 638
Hpy8I GTNNAC 2 cut(s) 469, 750
HpyAV CCTTC 3 cut(s) 429, 965, 1082
HpyCH4IV ACGT 2 cut(s) 134, 517
HpyCH4V TGCA 4 cut(s) 281, 536, 590, 928
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 116, 499, 921, 925
HpySE526I ACGT 2 cut(s) 134, 517
Hsp92I GRCGYC 1 cut(s) 688
Hsp92II CATG 5 cut(s) 110, 434, 590, 766, 1054
HspAI GCGC 3 cut(s) 569, 633, 1081
Kpn2I TCCGGA 2 cut(s) 137, 213
Kzo9I GATC 1 cut(s) 1003
LguI GCTCTTC 2 cut(s) 446, 489
LmnI GCTCC 3 cut(s) 838, 889, 897
Lsp1109I GCAGC 3 cut(s) 131, 199, 828
LweI GCATC 1 cut(s) 268
MaeI CTAG 2 cut(s) 111, 921
MaeII ACGT 2 cut(s) 134, 517
MaeIII GTNAC 2 cut(s) 341, 518
MalI GATC 1 cut(s) 1005
MboI GATC 1 cut(s) 1003
MhlI GDGCHC 2 cut(s) 425, 894
MlsI TGGCCA 1 cut(s) 585
MluCI AATT 6 cut(s) 58, 309, 323, 460, 694, 794
MluNI TGGCCA 1 cut(s) 585
MlyI GAGTC 3 cut(s) 112, 755, 809
MmeI TCCRAC 3 cut(s) 220, 919, 1039
Mox20I TGGCCA 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 592
MroI TCCGGA 2 cut(s) 137, 213
MroXI GAANNNNTTC 2 cut(s) 15, 359
MscI TGGCCA 1 cut(s) 585
MseI TTAA 3 cut(s) 459, 540, 779
Msp20I TGGCCA 1 cut(s) 585
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 9 cut(s) 82, 93, 138, 214, 419, 657, 683, 707, 728
MspR9I CCNGG 6 cut(s) 83, 419, 420, 657, 684, 728
MwoI GCNNNNNNNGC 5 cut(s) 45, 116, 499, 921, 925
NciI CCSGG 6 cut(s) 83, 419, 420, 657, 684, 728
NcoI CCATGG 1 cut(s) 430
NdeI CATATG 1 cut(s) 559
NdeII GATC 1 cut(s) 1003
NlaIII CATG 5 cut(s) 110, 434, 590, 766, 1054
NlaIV GGNNCC 2 cut(s) 150, 244
NmeAIII GCCGAG 1 cut(s) 452
NmuCI GTSAC 2 cut(s) 341, 518
NsiI ATGCAT 1 cut(s) 592
NspV TTCGAA 2 cut(s) 162, 353
PaeR7I CTCGAG 1 cut(s) 636
PciSI GCTCTTC 2 cut(s) 446, 489
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdmI GAANNNNTTC 2 cut(s) 15, 359
PfeI GAWTC 4 cut(s) 98, 679, 737, 1062
PkrI GCNGC 3 cut(s) 121, 189, 843
PleI GAGTC 3 cut(s) 111, 754, 808
PpsI GAGTC 3 cut(s) 111, 754, 808
Ppu21I YACGTR 1 cut(s) 518
PsiI TTATAA 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 894
PspFI CCCAGC 1 cut(s) 941
PspN4I GGNNCC 2 cut(s) 150, 244
PspPI GGNCC 2 cut(s) 38, 242
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 2 cut(s) 318, 1010
RsaNI GTAC 2 cut(s) 317, 1009
SacI GAGCTC 1 cut(s) 894
SapI GCTCTTC 2 cut(s) 446, 489
SaqAI TTAA 3 cut(s) 459, 540, 779
SatI GCNGC 3 cut(s) 120, 188, 842
Sau3AI GATC 1 cut(s) 1003
Sau96I GGNCC 2 cut(s) 38, 242
SchI GAGTC 3 cut(s) 112, 755, 809
ScrFI CCNGG 6 cut(s) 83, 419, 420, 657, 684, 728
SduI GDGCHC 2 cut(s) 425, 894
SfaNI GCATC 1 cut(s) 268
Sfr274I CTCGAG 1 cut(s) 636
SfuI TTCGAA 2 cut(s) 162, 353
SinI GGWCC 1 cut(s) 242
SlaI CTCGAG 1 cut(s) 636
SmaI CCCGGG 1 cut(s) 420
SmlI CTYRAG 3 cut(s) 507, 636, 1076
SmoI CTYRAG 3 cut(s) 507, 636, 1076
Sse9I AATT 6 cut(s) 58, 309, 323, 460, 694, 794
SsiI CCGC 4 cut(s) 185, 410, 710, 995
SspI AATATT 2 cut(s) 15, 159
SspMI CTAG 2 cut(s) 111, 921
SstI GAGCTC 1 cut(s) 894
StyD4I CCNGG 6 cut(s) 81, 417, 418, 655, 682, 726
StyI CCWWGG 2 cut(s) 430, 609
TaiI ACGT 2 cut(s) 137, 520
TaqI TCGA 6 cut(s) 101, 162, 171, 353, 637, 1013
TasI AATT 6 cut(s) 58, 309, 323, 460, 694, 794
TfiI GAWTC 4 cut(s) 98, 679, 737, 1062
Tru1I TTAA 3 cut(s) 459, 540, 779
Tru9I TTAA 3 cut(s) 459, 540, 779
TscAI CASTG 2 cut(s) 346, 571
TseFI GTSAC 2 cut(s) 341, 518
TseI GCWGC 3 cut(s) 119, 187, 841
Tsp45I GTSAC 2 cut(s) 341, 518
TspDTI ATGAA 6 cut(s) 24, 198, 244, 317, 476, 546
TspGWI ACGGA 1 cut(s) 820
TspMI CCCGGG 1 cut(s) 418
TspRI CASTG 2 cut(s) 346, 571
VpaK11BI GGWCC 1 cut(s) 242
XagI CCTNNNNNAGG 1 cut(s) 854
XapI RAATTY 4 cut(s) 58, 309, 323, 794
XhoI CTCGAG 1 cut(s) 636
XmaI CCCGGG 1 cut(s) 418
XmiI GTMKAC 2 cut(s) 468, 749
XmnI GAANNNNTTC 2 cut(s) 15, 359
XspI CTAG 2 cut(s) 111, 921
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.