Prupe.5G229100_v2.0.a1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0007511
Physical Location & Seq
Reverse (-)
17542261 .. 17544821
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G229100.1

Sequence Viewer

Length: 1068 bp
ATGGCAGAAGAATTTCCTATGACTGGTGGAGATGGCCCAAACAGCTACGCCAAAAATTCCATTTCACAGAGATTAGCAGCAGATGGTGCAAAGGGAATGTTGGTTGCATCAATATTCGAAAACCTTGACATTCAAACTCTGCCGTCTCGGCCTACTACTGCTTCAAGCACAATATTCCGGATTGCTGATTTAGGTTGCTCTACTGGACCTAACACATTCATTCAAGTAAAAAACATAATTGATGCCGTATCTCAAAAATACCACCAGTCTGCTGTTGAACTTCCAGAACTGCAAGTATACTTCAGTGACCTTGTTTCAAATGACTTCAATTATCTCTTTGCCACTCTCCCCCGAGATAGGCAATATTTCGCGGCAGGAGTTCCAGGCTCTTTTCATGGAAGACTCTTCCCTAAAGCCTCTCTTAATTTTGTCTACTCGGCATATGCTCTGCATTGGCTTTCGAAGCTGCCACAAGTGCTACGCGATGCCAACTCTCCTGCATTTAACAAGGGTAGGATTTTGTATGGCAATGCGCCTTATGAAGTTGGCCAGGCATATTCAGCTCAGTATGCCAAGGACATTGAGTGTTTTTTCCATGCTCGAGGACAAGAGCTTGCCCCTGGAGGCCTGATGGTGCTTTTGATTCCGGGGCGTCCCCATGGCACGCTTCCGGCACAGGGTTCTCTTGCCCCATTTTTTGCACCCTTGGAGTTTACTCTTGCTGATATGGTGAATGAGGGTTTATTAAGTGAGGATAAATTTGACTCTTTCAACTTGCCCTTCTACTGCCCATCTGTGGAGGAGTTGAGGACACTCATAGACGAAAATGGTTGCTTTGATATCTTGAAATTGGAGATCCAAGCCGCTCAGACGCCTGCATATCCTAGTGTACAACAATGTAGAGCTGGGATGGAGAGCATTCTCAAAAAACACTTCGGGGATGAAATTATAGAATTGCTTTTTGATCGATACTCGAAGAACAAAAATATTGCAGGAAGTGCTTCCCTTGTTGCTGATGATGGCCTGGCTGTTGGGTTCTTCGTTCTTGTCAAGCGCAAATATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

356

Amino Acids

39.06

Weight (kDa)

5.45

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 866
AccI GTMKAC 2 cut(s) 297, 432
AccII CGCG 2 cut(s) 371, 483
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 2 cut(s) 371, 864
AclWI GGATC 1 cut(s) 850
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 3 cut(s) 11, 55, 758
AcuI CTGAAG 1 cut(s) 286
AcyI GRCGYC 2 cut(s) 652, 872
AfaI GTAC 1 cut(s) 891
AfiI CCNNNNNNNGG 4 cut(s) 23, 357, 677, 796
AgsI TTSAA 8 cut(s) 134, 165, 224, 278, 318, 328, 772, 847
AjnI CCWGG 4 cut(s) 382, 549, 619, 1023
AluBI AGCT 5 cut(s) 45, 466, 563, 613, 905
AluI AGCT 5 cut(s) 45, 466, 563, 613, 905
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 1 cut(s) 850
Ama87I CYCGRG 2 cut(s) 351, 600
Aor13HI TCCGGA 1 cut(s) 177
AoxI GGCC 5 cut(s) 34, 149, 547, 625, 1021
ApeKI GCWGC 2 cut(s) 77, 466
ApoI RAATTY 3 cut(s) 11, 55, 758
Asp700I GAANNNNTTC 2 cut(s) 12, 1000
AspLEI GCGC 2 cut(s) 535, 1056
AspS9I GGNCC 2 cut(s) 35, 206
AsuC2I CCSGG 1 cut(s) 648
AsuHPI GGTGA 1 cut(s) 742
AsuII TTCGAA 2 cut(s) 117, 461
AvaI CYCGRG 2 cut(s) 351, 600
AvaII GGWCC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 549
BbsI GAAGAC 1 cut(s) 406
BbvI GCAGC 2 cut(s) 89, 453
BccI CCATC 6 cut(s) 26, 77, 625, 799, 904, 1013
BceAI ACGGC 2 cut(s) 127, 230
BciT130I CCWGG 4 cut(s) 384, 551, 621, 1025
BcnI CCSGG 1 cut(s) 648
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 1 cut(s) 885
BglI GCCNNNNNGGC 1 cut(s) 148
BisI GCNGC 4 cut(s) 78, 372, 467, 864
BlsI GCNGC 4 cut(s) 79, 373, 468, 865
Bme1390I CCNGG 5 cut(s) 384, 551, 621, 648, 1025
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 2 cut(s) 351, 600
BmgT120I GGNCC 2 cut(s) 35, 206
BmrFI CCNGG 5 cut(s) 384, 551, 621, 648, 1025
BmsI GCATC 3 cut(s) 116, 232, 475
BpiI GAAGAC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 642
Bpu14I TTCGAA 2 cut(s) 117, 461
BpuMI CCSGG 1 cut(s) 648
Bsa29I ATCGAT 1 cut(s) 967
BsaHI GRCGYC 2 cut(s) 652, 872
BsaJI CCNNGG 5 cut(s) 573, 619, 647, 658, 705
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 357, 677, 796
Bse1I ACTGG 3 cut(s) 28, 208, 265
Bse3DI GCAATG 1 cut(s) 535
BseAI TCCGGA 1 cut(s) 177
BseBI CCWGG 4 cut(s) 384, 551, 621, 1025
BseCI ATCGAT 1 cut(s) 967
BseDI CCNNGG 5 cut(s) 573, 619, 647, 658, 705
BseGI GGATG 2 cut(s) 915, 946
BseLI CCNNNNNNNGG 4 cut(s) 23, 357, 677, 796
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 2 cut(s) 578, 881
BseNI ACTGG 3 cut(s) 28, 208, 265
BseRI GAGGAG 1 cut(s) 815
BseXI GCAGC 2 cut(s) 89, 453
BseYI CCCAGC 1 cut(s) 905
Bsh1236I CGCG 2 cut(s) 371, 483
BshFI GGCC 5 cut(s) 36, 151, 549, 627, 1023
BshVI ATCGAT 1 cut(s) 967
BsiHKCI CYCGRG 2 cut(s) 351, 600
BsiSI CCGG 3 cut(s) 178, 647, 671
BslFI GGGAC 1 cut(s) 639
BslI CCNNNNNNNGG 4 cut(s) 23, 357, 677, 796
BsmAI GTCTC 1 cut(s) 150
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 639
BsmI GAATGC 1 cut(s) 918
BsnI GGCC 5 cut(s) 36, 151, 549, 627, 1023
BsoBI CYCGRG 2 cut(s) 351, 600
Bsp119I TTCGAA 2 cut(s) 117, 461
Bsp13I TCCGGA 1 cut(s) 177
Bsp1407I TGTACA 1 cut(s) 889
Bsp143I GATC 2 cut(s) 855, 964
Bsp19I CCATGG 1 cut(s) 658
BspACI CCGC 2 cut(s) 371, 864
BspANI GGCC 5 cut(s) 36, 151, 549, 627, 1023
BspCNI CTCAG 2 cut(s) 577, 880
BspDI ATCGAT 1 cut(s) 967
BspEI TCCGGA 1 cut(s) 177
BspFNI CGCG 2 cut(s) 371, 483
BspPI GGATC 1 cut(s) 850
BspT104I TTCGAA 2 cut(s) 117, 461
BsrBI CCGCTC 1 cut(s) 866
BsrDI GCAATG 1 cut(s) 535
BsrGI TGTACA 1 cut(s) 889
BsrI ACTGG 3 cut(s) 28, 208, 265
BssECI CCNNGG 5 cut(s) 573, 619, 647, 658, 705
BssMI GATC 2 cut(s) 855, 964
BssNAI GTATAC 1 cut(s) 298
BssNI GRCGYC 2 cut(s) 652, 872
BssT1I CCWWGG 3 cut(s) 573, 658, 705
Bst1107I GTATAC 1 cut(s) 298
Bst2UI CCWGG 4 cut(s) 384, 551, 621, 1025
Bst6I CTCTTC 1 cut(s) 410
BstACI GRCGYC 2 cut(s) 652, 872
BstAPI GCANNNNNTGC 2 cut(s) 86, 998
BstAUI TGTACA 1 cut(s) 889
BstBI TTCGAA 2 cut(s) 117, 461
BstC8I GCNNGC 3 cut(s) 615, 665, 876
BstDEI CTNAG 2 cut(s) 564, 867
BstDSI CCRYGG 1 cut(s) 658
BstF5I GGATG 2 cut(s) 915, 946
BstFNI CGCG 2 cut(s) 371, 483
BstHHI GCGC 2 cut(s) 535, 1056
BstKTI GATC 2 cut(s) 858, 967
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 2 cut(s) 855, 964
BstMWI GCNNNNNNNGC 8 cut(s) 42, 86, 148, 463, 475, 560, 569, 998
BstNI CCWGG 4 cut(s) 384, 551, 621, 1025
BstSCI CCNGG 5 cut(s) 382, 549, 619, 646, 1023
BstUI CGCG 2 cut(s) 371, 483
BstV1I GCAGC 2 cut(s) 89, 453
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 855
BstYI RGATCY 1 cut(s) 855
BstZ17I GTATAC 1 cut(s) 298
Bsu15I ATCGAT 1 cut(s) 967
BsuRI GGCC 5 cut(s) 36, 151, 549, 627, 1023
BsuTUI ATCGAT 1 cut(s) 967
BtgI CCRYGG 1 cut(s) 658
BtgZI GCGATG 1 cut(s) 498
BtsCI GGATG 2 cut(s) 915, 946
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 3 cut(s) 615, 665, 876
CfoI GCGC 2 cut(s) 535, 1056
Cfr13I GGNCC 2 cut(s) 35, 206
ClaI ATCGAT 1 cut(s) 967
CseI GACGC 2 cut(s) 641, 880
Csp6I GTAC 1 cut(s) 890
CviAII CATG 3 cut(s) 395, 596, 659
CviQI GTAC 1 cut(s) 890
DdeI CTNAG 2 cut(s) 564, 867
DpnI GATC 2 cut(s) 857, 966
DpnII GATC 2 cut(s) 855, 964
EaeI YGGCCR 1 cut(s) 547
Eam1104I CTCTTC 1 cut(s) 410
EarI CTCTTC 1 cut(s) 410
Eco130I CCWWGG 3 cut(s) 573, 658, 705
Eco147I AGGCCT 1 cut(s) 627
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 286
Eco88I CYCGRG 2 cut(s) 351, 600
EcoRII CCWGG 4 cut(s) 382, 549, 619, 1023
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 3 cut(s) 573, 658, 705
ErhI CCWWGG 3 cut(s) 573, 658, 705
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 3 cut(s) 398, 599, 662
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 1 cut(s) 639
FatI CATG 3 cut(s) 394, 595, 658
FauNDI CATATG 1 cut(s) 442
FblI GTMKAC 2 cut(s) 297, 432
Fnu4HI GCNGC 4 cut(s) 78, 372, 467, 864
FokI GGATG 2 cut(s) 922, 953
Fsp4HI GCNGC 4 cut(s) 78, 372, 467, 864
FspBI CTAG 1 cut(s) 885
GlaI GCGC 2 cut(s) 534, 1055
GluI GCNGC 4 cut(s) 78, 372, 467, 864
GsaI CCCAGC 1 cut(s) 909
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 5 cut(s) 36, 151, 549, 627, 1023
HapII CCGG 3 cut(s) 178, 647, 671
HgaI GACGC 2 cut(s) 641, 880
HhaI GCGC 2 cut(s) 535, 1056
Hin1I GRCGYC 2 cut(s) 652, 872
Hin1II CATG 3 cut(s) 398, 599, 662
Hin6I GCGC 2 cut(s) 533, 1054
HinP1I GCGC 2 cut(s) 533, 1054
HinfI GANTC 3 cut(s) 402, 643, 764
HpaII CCGG 3 cut(s) 178, 647, 671
HphI GGTGA 1 cut(s) 742
Hpy166II GTNNAC 4 cut(s) 298, 433, 714, 890
Hpy188I TCNGA 1 cut(s) 870
Hpy188III TCNNGA 3 cut(s) 178, 284, 844
Hpy8I GTNNAC 4 cut(s) 298, 433, 714, 890
HpyAV CCTTC 1 cut(s) 790
HpyCH4V TGCA 8 cut(s) 89, 107, 292, 451, 500, 701, 878, 992
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 86, 148, 463, 475, 560, 569, 998
HpyF3I CTNAG 2 cut(s) 564, 867
Hsp92I GRCGYC 2 cut(s) 652, 872
Hsp92II CATG 3 cut(s) 398, 599, 662
HspAI GCGC 2 cut(s) 533, 1054
Kpn2I TCCGGA 1 cut(s) 177
Kzo9I GATC 2 cut(s) 855, 964
Lsp1109I GCAGC 2 cut(s) 89, 453
LweI GCATC 3 cut(s) 116, 232, 475
MaeI CTAG 1 cut(s) 885
MaeIII GTNAC 1 cut(s) 305
MalI GATC 2 cut(s) 857, 966
MbiI CCGCTC 1 cut(s) 866
MboI GATC 2 cut(s) 855, 964
MboII GAAGA 5 cut(s) 20, 397, 411, 988, 1030
MflI RGATCY 1 cut(s) 855
MlsI TGGCCA 1 cut(s) 549
MluCI AATT 9 cut(s) 11, 55, 237, 328, 424, 758, 848, 945, 953
MluNI TGGCCA 1 cut(s) 549
MlyI GAGTC 2 cut(s) 396, 758
MnlI CCTC 7 cut(s) 427, 596, 617, 730, 745, 793, 801
Mox20I TGGCCA 1 cut(s) 549
MroI TCCGGA 1 cut(s) 177
MroXI GAANNNNTTC 2 cut(s) 12, 1000
MscI TGGCCA 1 cut(s) 549
MseI TTAA 3 cut(s) 423, 504, 746
Msp20I TGGCCA 1 cut(s) 549
MspI CCGG 3 cut(s) 178, 647, 671
MspR9I CCNGG 5 cut(s) 384, 551, 621, 648, 1025
Mva1269I GAATGC 1 cut(s) 918
MvaI CCWGG 4 cut(s) 384, 551, 621, 1025
MvnI CGCG 2 cut(s) 371, 483
MwoI GCNNNNNNNGC 8 cut(s) 42, 86, 148, 463, 475, 560, 569, 998
NciI CCSGG 1 cut(s) 648
NcoI CCATGG 1 cut(s) 658
NdeI CATATG 1 cut(s) 442
NdeII GATC 2 cut(s) 855, 964
NlaIII CATG 3 cut(s) 398, 599, 662
NmeAIII GCCGAG 2 cut(s) 127, 416
NmuCI GTSAC 1 cut(s) 305
NspV TTCGAA 2 cut(s) 117, 461
PaeR7I CTCGAG 1 cut(s) 600
PceI AGGCCT 1 cut(s) 627
PctI GAATGC 1 cut(s) 918
PdmI GAANNNNTTC 2 cut(s) 12, 1000
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 4 cut(s) 79, 373, 468, 865
PleI GAGTC 2 cut(s) 396, 758
PpsI GAGTC 2 cut(s) 396, 758
Psp6I CCWGG 4 cut(s) 382, 549, 619, 1023
PspFI CCCAGC 1 cut(s) 905
PspGI CCWGG 4 cut(s) 382, 549, 619, 1023
PspPI GGNCC 2 cut(s) 35, 206
PspXI VCTCGAGB 1 cut(s) 600
PsuI RGATCY 1 cut(s) 855
RsaI GTAC 1 cut(s) 891
RsaNI GTAC 1 cut(s) 890
SaqAI TTAA 3 cut(s) 423, 504, 746
SatI GCNGC 4 cut(s) 78, 372, 467, 864
Sau3AI GATC 2 cut(s) 855, 964
Sau96I GGNCC 2 cut(s) 35, 206
SchI GAGTC 2 cut(s) 396, 758
ScrFI CCNGG 5 cut(s) 384, 551, 621, 648, 1025
SetI ASST 9 cut(s) 47, 126, 196, 211, 312, 468, 565, 615, 907
SfaNI GCATC 3 cut(s) 116, 232, 475
Sfr274I CTCGAG 1 cut(s) 600
SfuI TTCGAA 2 cut(s) 117, 461
SinI GGWCC 1 cut(s) 206
SlaI CTCGAG 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 600
SmoI CTYRAG 1 cut(s) 600
Sse9I AATT 9 cut(s) 11, 55, 237, 328, 424, 758, 848, 945, 953
SseBI AGGCCT 1 cut(s) 627
SsiI CCGC 2 cut(s) 371, 864
SspI AATATT 4 cut(s) 114, 174, 365, 988
SspMI CTAG 1 cut(s) 885
StuI AGGCCT 1 cut(s) 627
StyD4I CCNGG 5 cut(s) 382, 549, 619, 646, 1023
StyI CCWWGG 3 cut(s) 573, 658, 705
TaqI TCGA 5 cut(s) 117, 461, 601, 967, 974
TasI AATT 9 cut(s) 11, 55, 237, 328, 424, 758, 848, 945, 953
TatI WGTACW 1 cut(s) 889
TauI GCSGC 2 cut(s) 374, 866
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 3 cut(s) 423, 504, 746
Tru9I TTAA 3 cut(s) 423, 504, 746
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 2 cut(s) 77, 466
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 4 cut(s) 208, 383, 555, 957
TspRI CASTG 1 cut(s) 310
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 3 cut(s) 11, 55, 758
XhoI CTCGAG 1 cut(s) 600
XmiI GTMKAC 2 cut(s) 297, 432
XmnI GAANNNNTTC 2 cut(s) 12, 1000
XspI CTAG 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.