RchiOBHm_Chr7g0178921

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007511
Physical Location & Seq
Forward (+)
1420544 .. 1423041
2498 bp
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UTR
Exon/CDS
Intron
PRQ15944

Sequence Viewer

Length: 492 bp
ATGGCAGAAGAAATGAGCCATACAACATCTGAAGGATACCCAATGAAAGGTGGGGATGGCGAATACAGCTACTTCATCCACTCTTCTCGTCAGAGATGTGCAGCTGATACTTCAAAGGGAACCCTAGTTGCAAAAATAGTCGAAAACCTTGCCTTCGAAAATCTGTCTTCAACCACGGTCCGAATTGCTGATCTAGGTTGTTCTGTTGGACCTAACACATTCATTGCAGTCGAGACCATCATTGAAGCGCAATACTTCGCTGCAGCAGTTCCGGGGAGTTTTCATGGAAGATTGTTTCCCAAGGCCTCTCTTGATATTGTTTACTCCGCATATGCTATCCAGTGGCTTTCTAAGACACCACAAGAGCTACTTGACTCGAACTCTCCTGCATTTAACAAAGGGAGGATTTTATATGGCAAATCACCTGATGAAGTTGCTCAAGCATATGGGGTTCAGTATGCCAAGGACATTCAGTGTTTTCTTCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

163

Amino Acids

17.78

Weight (kDa)

5.58

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 47 - 83 1.6e-09 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 84 - 163 6.4e-27 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 327
AcuI CTGAAG 1 cut(s) 51
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 3 cut(s) 114, 171, 245
AluBI AGCT 3 cut(s) 69, 104, 367
AluI AGCT 3 cut(s) 69, 104, 367
Alw26I GTCTC 1 cut(s) 227
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 3 cut(s) 101, 260, 263
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 2 cut(s) 178, 209
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 414
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 2 cut(s) 178, 209
BbsI GAAGAC 1 cut(s) 159
BbvI GCAGC 3 cut(s) 113, 247, 275
BccI CCATC 2 cut(s) 50, 245
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciVI GTATCC 1 cut(s) 29
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 2 cut(s) 125, 194
BfmI CTRYAG 1 cut(s) 261
BfuI GTATCC 1 cut(s) 29
BisI GCNGC 3 cut(s) 102, 261, 264
BlsI GCNGC 3 cut(s) 103, 262, 265
Bme1390I CCNGG 1 cut(s) 273
Bme18I GGWCC 2 cut(s) 178, 209
BmgT120I GGNCC 2 cut(s) 178, 209
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 4 cut(s) 174, 272, 300, 462
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 222
BseDI CCNNGG 4 cut(s) 174, 272, 300, 462
BseGI GGATG 2 cut(s) 61, 75
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 340
BseXI GCAGC 3 cut(s) 113, 247, 275
BsgI GTGCAG 1 cut(s) 120
BshFI GGCC 1 cut(s) 305
BsiSI CCGG 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 227
BsnI GGCC 1 cut(s) 305
Bso31I GGTCTC 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 156
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 1 cut(s) 327
BspANI GGCC 1 cut(s) 305
BspLI GGNNCC 1 cut(s) 121
BspMAI CTGCAG 1 cut(s) 265
BspT104I TTCGAA 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 222
BsrI ACTGG 1 cut(s) 340
BssECI CCNNGG 4 cut(s) 174, 272, 300, 462
BssMI GATC 1 cut(s) 190
BssT1I CCWWGG 2 cut(s) 300, 462
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 351
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 2 cut(s) 61, 75
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 3 cut(s) 113, 247, 275
BstV2I GAAGAC 1 cut(s) 159
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 2 cut(s) 61, 75
BtsIMutI CAGTG 2 cut(s) 347, 479
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 2 cut(s) 178, 209
CpoI CGGWCCG 1 cut(s) 178
CspI CGGWCCG 1 cut(s) 178
CviAII CATG 1 cut(s) 284
CviJI RGCY 6 cut(s) 18, 69, 104, 305, 346, 367
CviKI_1 RGCY 6 cut(s) 18, 69, 104, 305, 346, 367
DdeI CTNAG 1 cut(s) 351
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
Eco130I CCWWGG 2 cut(s) 300, 462
Eco147I AGGCCT 1 cut(s) 305
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 2 cut(s) 178, 209
Eco57I CTGAAG 1 cut(s) 51
EcoT14I CCWWGG 2 cut(s) 300, 462
ErhI CCWWGG 2 cut(s) 300, 462
FaeI CATG 1 cut(s) 287
FaiI YATR 9 cut(s) 21, 285, 331, 333, 412, 414, 445, 447, 459
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FatI CATG 1 cut(s) 283
FauNDI CATATG 2 cut(s) 331, 445
Fnu4HI GCNGC 3 cut(s) 102, 261, 264
FokI GGATG 2 cut(s) 62, 68
Fsp4HI GCNGC 3 cut(s) 102, 261, 264
FspBI CTAG 2 cut(s) 125, 194
GlaI GCGC 1 cut(s) 249
GluI GCNGC 3 cut(s) 102, 261, 264
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 272
HhaI GCGC 1 cut(s) 250
Hin1II CATG 1 cut(s) 287
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 1 cut(s) 374
HpaII CCGG 1 cut(s) 272
HphI GGTGA 1 cut(s) 414
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 3 cut(s) 31, 93, 182
Hpy188III TCNNGA 2 cut(s) 232, 311
Hpy8I GTNNAC 1 cut(s) 322
HpyAV CCTTC 2 cut(s) 26, 163
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4V TGCA 5 cut(s) 101, 131, 227, 263, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 66
HpyF3I CTNAG 1 cut(s) 351
Hsp92II CATG 1 cut(s) 287
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 1 cut(s) 190
LpnPI CCDG 4 cut(s) 285, 353, 399, 438
Lsp1109I GCAGC 3 cut(s) 113, 247, 275
MaeI CTAG 2 cut(s) 125, 194
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 5 cut(s) 20, 75, 159, 300, 473
MluCI AATT 1 cut(s) 183
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 2 cut(s) 316, 396
MseI TTAA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 66
NciI CCSGG 1 cut(s) 273
NdeI CATATG 2 cut(s) 331, 445
NdeII GATC 1 cut(s) 190
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 1 cut(s) 121
NspV TTCGAA 1 cut(s) 156
PceI AGGCCT 1 cut(s) 305
PkrI GCNGC 3 cut(s) 103, 262, 265
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 2 cut(s) 178, 209
PstI CTGCAG 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 104
Rsr2I CGGWCCG 1 cut(s) 178
RsrII CGGWCCG 1 cut(s) 178
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 3 cut(s) 102, 261, 264
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 2 cut(s) 178, 209
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 273
SetI ASST 8 cut(s) 52, 71, 106, 150, 199, 214, 369, 427
SfcI CTRYAG 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 2 cut(s) 178, 209
SmlI CTYRAG 1 cut(s) 438
SmoI CTYRAG 1 cut(s) 438
Sse9I AATT 1 cut(s) 183
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 1 cut(s) 327
SspMI CTAG 2 cut(s) 125, 194
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 1 cut(s) 271
StyI CCWWGG 2 cut(s) 300, 462
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 4 cut(s) 141, 156, 231, 377
TasI AATT 1 cut(s) 183
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 2 cut(s) 347, 479
TseI GCWGC 3 cut(s) 101, 260, 263
TspDTI ATGAA 5 cut(s) 59, 64, 211, 272, 444
TspRI CASTG 2 cut(s) 347, 479
VpaK11BI GGWCC 2 cut(s) 178, 209
XspI CTAG 2 cut(s) 125, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.