Rw7G001610

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007511
Physical Location & Seq
Forward (+)
1334506 .. 1335870
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001610.1

Sequence Viewer

Length: 996 bp
ATGGTGGGGATGGAAAGTACAGCTACTCTGTTCACTCTTATAATCAGGTACTCGAAAACCTTGACATTGAAACTCAGTCTCCTACTACAAGCTCATTCCGGATTGCTGAGTGCTGACTTAGGTTGTTCTGTTGGGCCTAATACATTTTCCGCAATCAACAACATCATTGAAGCTGTAGCACAAAAATACCAGCTTATTAAAGGCCACTCTGATCTCCCAGCAGAAATTCAAGTTTACTTTAGTGATTTGGTGTCAAATGATTTCAATCATCTCTTTGCCAGTCTCCCCCAAGATAGGCGATATTTTGCGGCTGGAGTTCCGGGATCTTTTCATGGAAGATTGTTTCCTAAAGCCTCCCTAGATTTTGTTTACTCTCAGTATGCTCTCCAATGGCTTTCTAGGGTACCACCAGAGATACTTGACTCCAACTCACCTGCATTCAACAAGGGGAGGATTATGTATGGAAATTCGGCTAGTGAAGTTGGTCAGGCATTTTCGGATCAGTATGCCAAGGACATGCAGTCTTTTTTTGATGCTCGAGCCCATGAGCTTGCTCCTGGAGGGTTATTGTTGCTCTTGATTCCAGGGCGTCCGGATGGAACTGTTCCGGCACAACATTCCGTACATCCGCTTTTTGAACCCATGGAGTCTAGTCTATCCGATATGGTTAATCAGGGTTTAATAAGTGGAGATAAACTTGACTCTTTCAACTTGCCCATCTACTCTCCATCCATGGAAGAGCTAAGGAAGATAATAGAAAACAGCGAGTGCTTCGATGTATTAAGGTTGGAGATCCAATTACCTGAGAGGCCAACTCCTTTGCGTAGTGAGTATGCTAGATCAGGCTTTGAGAAAATTCTCACCAAACACTTTGGGACTGAAATTATAGAACCACTTTTCGATCGATACTCCAAGAAAGTACAAGGTCGTTCTCCCATTACTTCTTGTGATGGTATGGCTGTTGGATTGTTCGTTCTTCTCAAGCGCAATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

331

Amino Acids

36.74

Weight (kDa)

5.92

Isoelectric Point (pI)

53.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 35 - 329 1.7e-92 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
AarI CACCTGC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 442
Acc65I GGTACC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 403
AccIII TCCGGA 2 cut(s) 98, 592
AciI CCGC 3 cut(s) 150, 308, 629
AclWI GGATC 3 cut(s) 331, 507, 787
AcsI RAATTY 3 cut(s) 225, 466, 855
AcyI GRCGYC 1 cut(s) 589
AfaI GTAC 5 cut(s) 19, 50, 405, 624, 921
AfiI CCNNNNNNNGG 1 cut(s) 294
AgsI TTSAA 7 cut(s) 70, 170, 230, 265, 442, 638, 709
AhdI GACNNNNNGTC 1 cut(s) 520
AjnI CCWGG 2 cut(s) 556, 583
AloI GAACNNNNNNTCC 2 cut(s) 957, 989
AluBI AGCT 6 cut(s) 23, 92, 173, 193, 550, 742
AluI AGCT 6 cut(s) 23, 92, 173, 193, 550, 742
Alw26I GTCTC 2 cut(s) 83, 287
AlwI GGATC 3 cut(s) 331, 507, 787
Ama87I CYCGRG 1 cut(s) 537
Aor13HI TCCGGA 2 cut(s) 98, 592
AoxI GGCC 3 cut(s) 134, 202, 809
ApoI RAATTY 3 cut(s) 225, 466, 855
Asp718I GGTACC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 987
AspS9I GGNCC 1 cut(s) 134
AsuC2I CCSGG 1 cut(s) 321
AsuHPI GGTGA 2 cut(s) 423, 853
AvaI CYCGRG 1 cut(s) 537
BanI GGYRCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 544
BccI CCATC 5 cut(s) 4, 590, 725, 736, 944
BciT130I CCWGG 2 cut(s) 558, 585
BcnI CCSGG 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 83, 287
BfaI CTAG 5 cut(s) 359, 399, 474, 651, 837
BfmI CTRYAG 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 1 cut(s) 309
BlsI GCNGC 1 cut(s) 310
Bme1390I CCNGG 3 cut(s) 321, 558, 585
BmeRI GACNNNNNGTC 1 cut(s) 520
BmeT110I CYCGRG 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 3 cut(s) 321, 558, 585
BmsI GCATC 1 cut(s) 523
BplI GAGNNNNNCTC 2 cut(s) 799, 831
BpmI CTGGAG 2 cut(s) 333, 579
Bpu10I CCTNAGC 1 cut(s) 743
BpuEI CTTGAG 1 cut(s) 965
BpuMI CCSGG 1 cut(s) 321
Bsa29I ATCGAT 1 cut(s) 904
BsaBI GATNNNNATC 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 589
BsaJI CCNNGG 4 cut(s) 510, 584, 642, 732
BsaWI WCCGGW 2 cut(s) 98, 592
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse1I ACTGG 1 cut(s) 279
Bse8I GATNNNNATC 1 cut(s) 264
BseAI TCCGGA 2 cut(s) 98, 592
BseBI CCWGG 2 cut(s) 558, 585
BseCI ATCGAT 1 cut(s) 904
BseDI CCNNGG 4 cut(s) 510, 584, 642, 732
BseGI GGATG 4 cut(s) 15, 601, 625, 728
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 4 cut(s) 88, 98, 389, 795
BseNI ACTGG 1 cut(s) 279
BseYI CCCAGC 1 cut(s) 217
Bsh1285I CGRYCG 1 cut(s) 904
BshFI GGCC 3 cut(s) 136, 204, 811
BshNI GGYRCC 1 cut(s) 403
BshVI ATCGAT 1 cut(s) 904
BsiEI CGRYCG 1 cut(s) 904
BsiHKCI CYCGRG 1 cut(s) 537
BsiSI CCGG 4 cut(s) 99, 320, 593, 608
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 1 cut(s) 294
BsmAI GTCTC 2 cut(s) 83, 287
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 3 cut(s) 136, 204, 811
BsoBI CYCGRG 1 cut(s) 537
Bsp1286I GDGCHC 1 cut(s) 544
Bsp13I TCCGGA 2 cut(s) 98, 592
Bsp143I GATC 6 cut(s) 211, 323, 499, 792, 839, 901
Bsp19I CCATGG 2 cut(s) 642, 732
BspACI CCGC 3 cut(s) 150, 308, 629
BspANI GGCC 3 cut(s) 136, 204, 811
BspCNI CTCAG 4 cut(s) 87, 99, 388, 796
BspDI ATCGAT 1 cut(s) 904
BspEI TCCGGA 2 cut(s) 98, 592
BspLI GGNNCC 1 cut(s) 405
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 3 cut(s) 331, 507, 787
BspQI GCTCTTC 1 cut(s) 732
BspT107I GGYRCC 1 cut(s) 403
BsrI ACTGG 1 cut(s) 279
BssECI CCNNGG 4 cut(s) 510, 584, 642, 732
BssMI GATC 6 cut(s) 211, 323, 499, 792, 839, 901
BssNI GRCGYC 1 cut(s) 589
BssT1I CCWWGG 3 cut(s) 510, 642, 732
Bst2UI CCWGG 2 cut(s) 558, 585
Bst4CI ACNGT 1 cut(s) 604
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 6 cut(s) 74, 107, 118, 375, 743, 804
BstDSI CCRYGG 2 cut(s) 642, 732
BstF5I GGATG 4 cut(s) 15, 601, 625, 728
BstHHI GCGC 1 cut(s) 987
BstKTI GATC 6 cut(s) 214, 326, 502, 795, 842, 904
BstMAI GTCTC 2 cut(s) 83, 287
BstMBI GATC 6 cut(s) 211, 323, 499, 792, 839, 901
BstMCI CGRYCG 1 cut(s) 904
BstNI CCWGG 2 cut(s) 558, 585
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 3 cut(s) 319, 556, 583
BstSFI CTRYAG 1 cut(s) 174
BstX2I RGATCY 2 cut(s) 323, 792
BstYI RGATCY 2 cut(s) 323, 792
Bsu15I ATCGAT 1 cut(s) 904
BsuRI GGCC 3 cut(s) 136, 204, 811
BsuTUI ATCGAT 1 cut(s) 904
BtgI CCRYGG 2 cut(s) 642, 732
BtsCI GGATG 4 cut(s) 15, 601, 625, 728
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 552
CfoI GCGC 1 cut(s) 987
Cfr13I GGNCC 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 904
CseI GACGC 1 cut(s) 578
Csp6I GTAC 5 cut(s) 18, 49, 404, 623, 920
CviAII CATG 5 cut(s) 332, 517, 545, 643, 733
CviQI GTAC 5 cut(s) 18, 49, 404, 623, 920
DdeI CTNAG 6 cut(s) 74, 107, 118, 375, 743, 804
DpnI GATC 6 cut(s) 213, 325, 501, 794, 841, 903
DpnII GATC 6 cut(s) 211, 323, 499, 792, 839, 901
DriI GACNNNNNGTC 1 cut(s) 520
Eam1104I CTCTTC 1 cut(s) 732
Eam1105I GACNNNNNGTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 732
Eco130I CCWWGG 3 cut(s) 510, 642, 732
Eco24I GRGCYC 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 537
EcoRII CCWGG 2 cut(s) 556, 583
EcoT14I CCWWGG 3 cut(s) 510, 642, 732
EcoT38I GRGCYC 1 cut(s) 544
ErhI CCWWGG 3 cut(s) 510, 642, 732
FaeI CATG 5 cut(s) 335, 520, 548, 646, 736
FaqI GGGAC 1 cut(s) 889
FatI CATG 5 cut(s) 331, 516, 544, 642, 732
Fnu4HI GCNGC 1 cut(s) 309
FokI GGATG 4 cut(s) 22, 608, 612, 715
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 1 cut(s) 309
FspBI CTAG 5 cut(s) 359, 399, 474, 651, 837
GlaI GCGC 1 cut(s) 986
GluI GCNGC 1 cut(s) 309
GsaI CCCAGC 1 cut(s) 221
GsuI CTGGAG 2 cut(s) 333, 579
HaeIII GGCC 3 cut(s) 136, 204, 811
HapII CCGG 4 cut(s) 99, 320, 593, 608
HgaI GACGC 1 cut(s) 578
HhaI GCGC 1 cut(s) 987
Hin1I GRCGYC 1 cut(s) 589
Hin1II CATG 5 cut(s) 335, 520, 548, 646, 736
Hin6I GCGC 1 cut(s) 985
HinP1I GCGC 1 cut(s) 985
HinfI GANTC 4 cut(s) 422, 580, 647, 701
HpaII CCGG 4 cut(s) 99, 320, 593, 608
HphI GGTGA 2 cut(s) 423, 853
Hpy166II GTNNAC 3 cut(s) 33, 235, 370
Hpy188I TCNGA 3 cut(s) 211, 499, 661
Hpy188III TCNNGA 3 cut(s) 99, 577, 593
Hpy8I GTNNAC 3 cut(s) 33, 235, 370
HpyCH4III ACNGT 1 cut(s) 604
HpyCH4V TGCA 2 cut(s) 437, 520
HpyF3I CTNAG 6 cut(s) 74, 107, 118, 375, 743, 804
Hsp92I GRCGYC 1 cut(s) 589
Hsp92II CATG 5 cut(s) 335, 520, 548, 646, 736
HspAI GCGC 1 cut(s) 985
Kpn2I TCCGGA 2 cut(s) 98, 592
KpnI GGTACC 1 cut(s) 407
Kzo9I GATC 6 cut(s) 211, 323, 499, 792, 839, 901
LguI GCTCTTC 1 cut(s) 732
LmnI GCTCC 1 cut(s) 559
LweI GCATC 1 cut(s) 523
MaeI CTAG 5 cut(s) 359, 399, 474, 651, 837
MalI GATC 6 cut(s) 213, 325, 501, 794, 841, 903
MboI GATC 6 cut(s) 211, 323, 499, 792, 839, 901
MboII GAAGA 4 cut(s) 348, 749, 760, 968
MflI RGATCY 2 cut(s) 323, 792
MhlI GDGCHC 1 cut(s) 544
MluCI AATT 5 cut(s) 225, 466, 797, 855, 882
MlyI GAGTC 3 cut(s) 416, 656, 695
MmeI TCCRAC 3 cut(s) 450, 768, 943
MnlI CCTC 4 cut(s) 364, 444, 554, 801
MroI TCCGGA 2 cut(s) 98, 592
MseI TTAA 5 cut(s) 198, 669, 680, 782, 994
MspI CCGG 4 cut(s) 99, 320, 593, 608
MspR9I CCNGG 3 cut(s) 321, 558, 585
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 2 cut(s) 558, 585
NciI CCSGG 1 cut(s) 321
NcoI CCATGG 2 cut(s) 642, 732
NdeII GATC 6 cut(s) 211, 323, 499, 792, 839, 901
NlaIII CATG 5 cut(s) 335, 520, 548, 646, 736
NlaIV GGNNCC 1 cut(s) 405
NspI RCATGY 1 cut(s) 520
PaeR7I CTCGAG 1 cut(s) 537
PaqCI CACCTGC 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 732
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 1 cut(s) 580
PfoI TCCNGGA 2 cut(s) 319, 556
PkrI GCNGC 1 cut(s) 310
Ple19I CGATCG 1 cut(s) 904
PleI GAGTC 3 cut(s) 416, 655, 695
PpsI GAGTC 3 cut(s) 416, 655, 695
PsiI TTATAA 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 556, 583
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 2 cut(s) 556, 583
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 134
PspXI VCTCGAGB 1 cut(s) 537
PsuI RGATCY 2 cut(s) 323, 792
PvuI CGATCG 1 cut(s) 904
RsaI GTAC 5 cut(s) 19, 50, 405, 624, 921
RsaNI GTAC 5 cut(s) 18, 49, 404, 623, 920
SapI GCTCTTC 1 cut(s) 732
SaqAI TTAA 5 cut(s) 198, 669, 680, 782, 994
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 6 cut(s) 211, 323, 499, 792, 839, 901
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 3 cut(s) 416, 656, 695
ScrFI CCNGG 3 cut(s) 321, 558, 585
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 1 cut(s) 523
SfcI CTRYAG 1 cut(s) 174
Sfr274I CTCGAG 1 cut(s) 537
SlaI CTCGAG 1 cut(s) 537
SmlI CTYRAG 2 cut(s) 537, 980
SmoI CTYRAG 2 cut(s) 537, 980
Sse9I AATT 5 cut(s) 225, 466, 797, 855, 882
SsiI CCGC 3 cut(s) 150, 308, 629
SspMI CTAG 5 cut(s) 359, 399, 474, 651, 837
StyD4I CCNGG 3 cut(s) 319, 556, 583
StyI CCWWGG 3 cut(s) 510, 642, 732
TaaI ACNGT 1 cut(s) 604
TaqI TCGA 5 cut(s) 53, 538, 774, 900, 904
TasI AATT 5 cut(s) 225, 466, 797, 855, 882
TatI WGTACW 2 cut(s) 17, 919
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 1 cut(s) 580
Tru1I TTAA 5 cut(s) 198, 669, 680, 782, 994
Tru9I TTAA 5 cut(s) 198, 669, 680, 782, 994
TspDTI ATGAA 1 cut(s) 320
TspGWI ACGGA 1 cut(s) 610
XapI RAATTY 3 cut(s) 225, 466, 855
XceI RCATGY 1 cut(s) 520
XhoI CTCGAG 1 cut(s) 537
XspI CTAG 5 cut(s) 359, 399, 474, 651, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.