MD06G1224000.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007511
Physical Location & Seq
Reverse (-)
35460814 .. 35462683
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1224000.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGATCAAGAATCATTTCCTATGGCTGGAGGAGACGACCAATACAGCTACAGTAAAAATTCCGGTGCACAGGGGAAGGCAGCCGATACTGTCAAGGGAATGCTGATTGATGCCATACTTGAAAACCTACACATTGGAAATCATCTGTCACAAGGAAATGTTGTTTCAAACTCATCATTTTCCATTGCTGATCTAGGTTGTTCTGTTGGCCCCAACACATTCATCGCGATCGACAATATTATGGAGACTGTGACACAAAAAATCAAGAACGAAGGCCAATCTTCTTCGCTCCCGGAATTCCATGTCTTCTTCAACGACCATGTTTCAAACGATTTCAACACTCTCTTCGTCAACCTTCCCCCGAATAGGCAATACTTCGCAGTTGGAGTTCCCGGATCATTTTATGGAAGATTGTTCCCCAAGGCATCCCTTAATTTTGTTTACTCTGCGTACTCCGTCATTTGGCTTTCTAAGGTACCTGAAGGCATAGCCGACTTGAATTCACCTATATTAAACAAGGGAAGGATGTACTATTCGAATGCGCCTATTGAAGTTGGTCACGCATATTCAGTTCAGTATGCAAAAGACATGAAGTGTTTTCTGGATGCTCGAGCGGAAGAGCTCGCTCCTAGAGGCTTACTGGTGCTTCTTGTTACCGGTCGTCCGGATGGGACGCTTCCAAGGGAATGTTCTTGTGGCCCGCTTTACGAACGAGTTGAATCTTGCCTTATAGATATGGTAAATGAGGGGTTAATAAGCCAAGACAAACTGGACACTTTCAACCATCCAACGTACAGCCCATCAATGGAGGAGCTGAGTGAGCTAGTACACGAAAACGGGTGTTTTGAGATTGCAAGACTGGAAAAGCAACCTCGGGTGTCCCCCTCTTATAGTACAACAGGAGAGGTTAGAGCCGGCATGGGAAGCCTTCTCACAAACCACTTTGGCTCTGAAATTTTAGATTCGCTTTTCGATCGGTACTCAAAAAAAATCGAAGCACAACCGCGTCCGGTCCTCACTGCTGGTGTTGATGATATGGCTTACACATTATTTGTCGTTCTCAAGCGAAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

359

Amino Acids

39.51

Weight (kDa)

5.23

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 56 - 356 2.1e-97 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 475
AccB1I GGYRCC 1 cut(s) 475
AccBSI CCGCTC 1 cut(s) 614
AccII CGCG 2 cut(s) 227, 1006
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 3 cut(s) 614, 701, 1004
AclWI GGATC 2 cut(s) 12, 403
AcsI RAATTY 4 cut(s) 58, 296, 499, 954
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 7 cut(s) 452, 477, 530, 794, 828, 895, 980
AfiI CCNNNNNNNGG 4 cut(s) 26, 366, 462, 805
AgeI ACCGGT 1 cut(s) 656
AgsI TTSAA 9 cut(s) 122, 168, 313, 327, 337, 499, 551, 719, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 673, 705
AluBI AGCT 4 cut(s) 48, 622, 814, 823
AluI AGCT 4 cut(s) 48, 622, 814, 823
Alw21I GWGCWC 2 cut(s) 70, 624
Alw26I GTCTC 2 cut(s) 27, 239
Alw44I GTGCAC 1 cut(s) 66
AlwI GGATC 2 cut(s) 12, 403
Ama87I CYCGRG 2 cut(s) 609, 873
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 3 cut(s) 208, 274, 697
ApaLI GTGCAC 1 cut(s) 66
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 4 cut(s) 58, 296, 499, 954
AsiGI ACCGGT 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 15
Asp718I GGTACC 1 cut(s) 475
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 3 cut(s) 209, 698, 1012
AsuC2I CCSGG 2 cut(s) 293, 393
AsuHPI GGTGA 1 cut(s) 495
AsuII TTCGAA 1 cut(s) 536
AvaI CYCGRG 2 cut(s) 609, 873
AvaII GGWCC 1 cut(s) 1012
BaeGI GKGCMC 1 cut(s) 70
BanI GGYRCC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 624
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 2 cut(s) 70, 624
BbvI GCAGC 1 cut(s) 92
BccI CCATC 3 cut(s) 662, 792, 808
BcnI CCSGG 2 cut(s) 293, 393
BcoDI GTCTC 2 cut(s) 27, 239
BfaI CTAG 3 cut(s) 194, 630, 824
BfmI CTRYAG 1 cut(s) 49
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 2 cut(s) 293, 393
Bme18I GGWCC 1 cut(s) 1012
BmeT110I CYCGRG 2 cut(s) 609, 873
BmgT120I GGNCC 3 cut(s) 209, 698, 1012
BmiI GGNNCC 2 cut(s) 211, 477
BmrFI CCNGG 2 cut(s) 293, 393
BmsI GCATC 3 cut(s) 100, 434, 595
BpiI GAAGAC 1 cut(s) 298
BpmI CTGGAG 1 cut(s) 48
Bpu14I TTCGAA 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 1046
BpuMI CCSGG 2 cut(s) 293, 393
BsaJI CCNNGG 3 cut(s) 420, 680, 872
BsaWI WCCGGW 4 cut(s) 62, 656, 664, 1009
Bsc4I CCNNNNNNNGG 4 cut(s) 26, 366, 462, 805
Bse118I RCCGGY 2 cut(s) 656, 914
Bse1I ACTGG 3 cut(s) 645, 774, 864
Bse3DI GCAATG 1 cut(s) 183
BseAI TCCGGA 1 cut(s) 664
BseDI CCNNGG 3 cut(s) 420, 680, 872
BseGI GGATG 5 cut(s) 425, 531, 610, 673, 784
BseLI CCNNNNNNNGG 4 cut(s) 26, 366, 462, 805
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 806
BseNI ACTGG 3 cut(s) 645, 774, 864
BseRI GAGGAG 2 cut(s) 45, 824
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 1 cut(s) 92
Bsh1236I CGCG 2 cut(s) 227, 1006
Bsh1285I CGRYCG 3 cut(s) 231, 661, 976
BshFI GGCC 3 cut(s) 210, 276, 699
BshNI GGYRCC 1 cut(s) 475
BshTI ACCGGT 1 cut(s) 656
BsiEI CGRYCG 3 cut(s) 231, 661, 976
BsiHKAI GWGCWC 2 cut(s) 70, 624
BsiHKCI CYCGRG 2 cut(s) 609, 873
BsiSI CCGG 7 cut(s) 63, 293, 393, 657, 665, 915, 1010
BslFI GGGAC 2 cut(s) 685, 865
BslI CCNNNNNNNGG 4 cut(s) 26, 366, 462, 805
BsmAI GTCTC 2 cut(s) 27, 239
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 685, 865
BsmI GAATGC 2 cut(s) 105, 544
BsnI GGCC 3 cut(s) 210, 276, 699
BsoBI CYCGRG 2 cut(s) 609, 873
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 70, 624
Bsp13I TCCGGA 1 cut(s) 664
Bsp143I GATC 5 cut(s) 4, 190, 228, 395, 973
Bsp68I TCGCGA 1 cut(s) 227
BspACI CCGC 3 cut(s) 614, 701, 1004
BspANI GGCC 3 cut(s) 210, 276, 699
BspCNI CTCAG 1 cut(s) 807
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 2 cut(s) 227, 1006
BspLI GGNNCC 2 cut(s) 211, 477
BspPI GGATC 2 cut(s) 12, 403
BspQI GCTCTTC 1 cut(s) 612
BspT104I TTCGAA 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 475
BsrBI CCGCTC 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 183
BsrFI RCCGGY 2 cut(s) 656, 914
BsrI ACTGG 3 cut(s) 645, 774, 864
BssAI RCCGGY 2 cut(s) 656, 914
BssECI CCNNGG 3 cut(s) 420, 680, 872
BssMI GATC 5 cut(s) 4, 190, 228, 395, 973
BssT1I CCWWGG 2 cut(s) 420, 680
Bst4CI ACNGT 3 cut(s) 53, 91, 250
Bst6I CTCTTC 2 cut(s) 350, 612
BstBI TTCGAA 1 cut(s) 536
BstC8I GCNNGC 3 cut(s) 624, 701, 916
BstDEI CTNAG 2 cut(s) 471, 815
BstF5I GGATG 5 cut(s) 425, 531, 610, 673, 784
BstFNI CGCG 2 cut(s) 227, 1006
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 5 cut(s) 7, 193, 231, 398, 976
BstMAI GTCTC 2 cut(s) 27, 239
BstMBI GATC 5 cut(s) 4, 190, 228, 395, 973
BstMCI CGRYCG 3 cut(s) 231, 661, 976
BstMWI GCNNNNNNNGC 2 cut(s) 820, 924
BstSCI CCNGG 2 cut(s) 291, 391
BstSFI CTRYAG 1 cut(s) 49
BstSLI GKGCMC 1 cut(s) 70
BstUI CGCG 2 cut(s) 227, 1006
BstV1I GCAGC 1 cut(s) 92
BstV2I GAAGAC 1 cut(s) 298
BsuRI GGCC 3 cut(s) 210, 276, 699
BtgZI GCGATG 1 cut(s) 208
BtsCI GGATG 5 cut(s) 425, 531, 610, 673, 784
BtsI GCAGTG 1 cut(s) 1017
BtsIMutI CAGTG 1 cut(s) 1017
BtuMI TCGCGA 1 cut(s) 227
Cac8I GCNNGC 3 cut(s) 624, 701, 916
CfoI GCGC 1 cut(s) 544
Cfr10I RCCGGY 2 cut(s) 656, 914
Cfr13I GGNCC 3 cut(s) 209, 698, 1012
CseI GACGC 2 cut(s) 682, 995
Csp6I GTAC 7 cut(s) 451, 476, 529, 793, 827, 894, 979
CspAI ACCGGT 1 cut(s) 656
CviAII CATG 4 cut(s) 302, 320, 589, 919
CviQI GTAC 7 cut(s) 451, 476, 529, 793, 827, 894, 979
DdeI CTNAG 2 cut(s) 471, 815
DpnI GATC 5 cut(s) 6, 192, 230, 397, 975
DpnII GATC 5 cut(s) 4, 190, 228, 395, 973
Eam1104I CTCTTC 2 cut(s) 350, 612
EarI CTCTTC 2 cut(s) 350, 612
Ecl136II GAGCTC 1 cut(s) 622
Eco130I CCWWGG 2 cut(s) 420, 680
Eco24I GRGCYC 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 1012
Eco53kI GAGCTC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 2 cut(s) 609, 873
EcoICRI GAGCTC 1 cut(s) 622
EcoRI GAATTC 2 cut(s) 296, 499
EcoT14I CCWWGG 2 cut(s) 420, 680
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 2 cut(s) 420, 680
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 4 cut(s) 305, 323, 592, 922
FaqI GGGAC 2 cut(s) 685, 865
FatI CATG 4 cut(s) 301, 319, 588, 918
FauI CCCGC 1 cut(s) 708
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 5 cut(s) 412, 538, 617, 680, 771
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 3 cut(s) 194, 630, 824
GlaI GCGC 1 cut(s) 543
GluI GCNGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 3 cut(s) 210, 276, 699
HapII CCGG 7 cut(s) 63, 293, 393, 657, 665, 915, 1010
HgaI GACGC 2 cut(s) 682, 995
HhaI GCGC 1 cut(s) 544
Hin1II CATG 4 cut(s) 305, 323, 592, 922
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 3 cut(s) 11, 719, 962
HpaII CCGG 7 cut(s) 63, 293, 393, 657, 665, 915, 1010
HphI GGTGA 1 cut(s) 495
Hpy166II GTNNAC 4 cut(s) 68, 352, 442, 829
Hpy188I TCNGA 1 cut(s) 952
Hpy188III TCNNGA 5 cut(s) 8, 226, 265, 602, 665
Hpy8I GTNNAC 4 cut(s) 68, 352, 442, 829
HpyAV CCTTC 6 cut(s) 70, 266, 365, 476, 516, 938
HpyCH4III ACNGT 3 cut(s) 53, 91, 250
HpyCH4IV ACGT 1 cut(s) 791
HpyCH4V TGCA 3 cut(s) 68, 581, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 820, 924
HpyF3I CTNAG 2 cut(s) 471, 815
HpySE526I ACGT 1 cut(s) 791
Hsp92II CATG 4 cut(s) 305, 323, 592, 922
HspAI GCGC 1 cut(s) 542
Kpn2I TCCGGA 1 cut(s) 664
KpnI GGTACC 1 cut(s) 479
KroI GCCGGC 1 cut(s) 914
KroNI GCCGGC 1 cut(s) 916
Kzo9I GATC 5 cut(s) 4, 190, 228, 395, 973
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 3 cut(s) 294, 631, 811
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 3 cut(s) 100, 434, 595
MaeI CTAG 3 cut(s) 194, 630, 824
MaeII ACGT 1 cut(s) 791
MaeIII GTNAC 4 cut(s) 147, 250, 557, 652
MalI GATC 5 cut(s) 6, 192, 230, 397, 975
MbiI CCGCTC 1 cut(s) 614
MboI GATC 5 cut(s) 4, 190, 228, 395, 973
MboII GAAGA 7 cut(s) 273, 276, 298, 301, 337, 420, 629
MhlI GDGCHC 2 cut(s) 70, 624
MluCI AATT 5 cut(s) 58, 296, 433, 499, 954
MmeI TCCRAC 2 cut(s) 364, 812
MnlI CCTC 8 cut(s) 23, 626, 739, 802, 882, 895, 898, 1025
MroI TCCGGA 1 cut(s) 664
MroNI GCCGGC 1 cut(s) 914
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 3 cut(s) 432, 512, 752
MspI CCGG 7 cut(s) 63, 293, 393, 657, 665, 915, 1010
MspR9I CCNGG 2 cut(s) 293, 393
Mva1269I GAATGC 2 cut(s) 105, 544
MvnI CGCG 2 cut(s) 227, 1006
MwoI GCNNNNNNNGC 2 cut(s) 820, 924
NaeI GCCGGC 1 cut(s) 916
NciI CCSGG 2 cut(s) 293, 393
NdeII GATC 5 cut(s) 4, 190, 228, 395, 973
NgoMIV GCCGGC 1 cut(s) 914
NlaIII CATG 4 cut(s) 305, 323, 592, 922
NlaIV GGNNCC 2 cut(s) 211, 477
NmuCI GTSAC 3 cut(s) 147, 250, 557
NruI TCGCGA 1 cut(s) 227
NspV TTCGAA 1 cut(s) 536
PaeR7I CTCGAG 1 cut(s) 609
PciSI GCTCTTC 1 cut(s) 612
PctI GAATGC 2 cut(s) 105, 544
PdiI GCCGGC 1 cut(s) 916
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 3 cut(s) 11, 719, 962
PfoI TCCNGGA 2 cut(s) 291, 391
PinAI ACCGGT 1 cut(s) 656
PkrI GCNGC 1 cut(s) 82
Ple19I CGATCG 2 cut(s) 231, 976
Psp124BI GAGCTC 1 cut(s) 624
PspN4I GGNNCC 2 cut(s) 211, 477
PspPI GGNCC 3 cut(s) 209, 698, 1012
PspXI VCTCGAGB 1 cut(s) 609
PvuI CGATCG 2 cut(s) 231, 976
RruI TCGCGA 1 cut(s) 227
RsaI GTAC 7 cut(s) 452, 477, 530, 794, 828, 895, 980
RsaNI GTAC 7 cut(s) 451, 476, 529, 793, 827, 894, 979
SacI GAGCTC 1 cut(s) 624
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 3 cut(s) 432, 512, 752
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 5 cut(s) 4, 190, 228, 395, 973
Sau96I GGNCC 3 cut(s) 209, 698, 1012
ScrFI CCNGG 2 cut(s) 293, 393
SduI GDGCHC 2 cut(s) 70, 624
SfaNI GCATC 3 cut(s) 100, 434, 595
SfcI CTRYAG 1 cut(s) 49
Sfr274I CTCGAG 1 cut(s) 609
SfuI TTCGAA 1 cut(s) 536
SinI GGWCC 1 cut(s) 1012
SlaI CTCGAG 1 cut(s) 609
SmlI CTYRAG 2 cut(s) 609, 1061
SmoI CTYRAG 2 cut(s) 609, 1061
Sse9I AATT 5 cut(s) 58, 296, 433, 499, 954
SsiI CCGC 3 cut(s) 614, 701, 1004
SspI AATATT 1 cut(s) 238
SspMI CTAG 3 cut(s) 194, 630, 824
SstI GAGCTC 1 cut(s) 624
StyD4I CCNGG 2 cut(s) 291, 391
StyI CCWWGG 2 cut(s) 420, 680
TaaI ACNGT 3 cut(s) 53, 91, 250
TaiI ACGT 1 cut(s) 794
TaqI TCGA 5 cut(s) 231, 536, 610, 972, 993
TasI AATT 5 cut(s) 58, 296, 433, 499, 954
TatI WGTACW 3 cut(s) 528, 826, 893
TfiI GAWTC 3 cut(s) 11, 719, 962
Tru1I TTAA 3 cut(s) 432, 512, 752
Tru9I TTAA 3 cut(s) 432, 512, 752
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 3 cut(s) 147, 250, 557
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 3 cut(s) 147, 250, 557
TspDTI ATGAA 2 cut(s) 211, 605
TspGWI ACGGA 1 cut(s) 445
TspRI CASTG 1 cut(s) 1024
VneI GTGCAC 1 cut(s) 66
VpaK11BI GGWCC 1 cut(s) 1012
XapI RAATTY 4 cut(s) 58, 296, 499, 954
XhoI CTCGAG 1 cut(s) 609
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 3 cut(s) 194, 630, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.