FvH4_5g18720

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004776
Physical Location & Seq
Reverse (-)
10759586 .. 10761174
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18720.t2

Sequence Viewer

Length: 678 bp
ATGGCTTCGTCAATCTCAATCGCTCTCAGAAGGGCATCATCGTCCCCAAGCCTCTTCTCCAAGCTGTTCAACCCTCTTCGCTCTGTTTCTGTAGCCCCCTCTGTTTCTCGCTCCTTCAACACCAATGCCCAGAGGTCCAGCTACGACGACGATGACCGCAGCGTTTCCGTCGACCGCTCCACCTCCGGCTCTCCCTATCGCCGCCGTGACCCTATAGCAGATGTATTTGATCCATTTTATGACGAGTCGCGGAGCATTGCAAGAAGTCTGAACCAGGTGCCAAGAAGTCTGAGCCAGGTCCTCAACTTGATGGACCAATTCATGGAGAATCCATACCTGGCTGCTTATAGGGGCCTCGGCGCAGGTGGATCAAGAAGAGGCTGGGACGTAAAGGAAACCGAGGACTCATTGCTGCTGAGAATGGACATGCCGGGGTTGAACAAGGAGGATGTGAAGATCTCAGTGGAGCAGGGCACTCTGACTGTGAAAGGAGAAGGAAAGGACCCTGAGGGTGAGGAAGATGGAGGGAGGAGGTTCTCTACCAGATTGGATCTGCCTGCTAAGATTTATGAGCTCAATTCGATCAAGGCTGAGATGAAGAATGGGGTTCTCAAGTTGGTGGTTCCTAAGGTGAAAGAGGAGGAGAAGAAGAATGTGTTTGAGGTGAAGATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

226

Amino Acids

25.12

Weight (kDa)

7.83

Isoelectric Point (pI)

67.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 128 - 223 2.6e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 353
AccB1I GGYRCC 1 cut(s) 277
AccB7I CCANNNNNTGG 1 cut(s) 322
AccBSI CCGCTC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 171
AccII CGCG 1 cut(s) 250
AciI CCGC 4 cut(s) 157, 175, 202, 250
AclWI GGATC 3 cut(s) 224, 376, 558
AfiI CCNNNNNNNGG 1 cut(s) 322
AgsI TTSAA 4 cut(s) 70, 118, 439, 674
AjnI CCWGG 3 cut(s) 273, 294, 336
AluBI AGCT 3 cut(s) 64, 141, 574
AluI AGCT 3 cut(s) 64, 141, 574
Alw21I GWGCWC 1 cut(s) 576
AlwI GGATC 3 cut(s) 224, 376, 558
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 3 cut(s) 159, 341, 412
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 5 cut(s) 135, 298, 313, 352, 502
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 3 cut(s) 524, 643, 676
AvaII GGWCC 4 cut(s) 135, 298, 313, 502
AxyI CCTNAGG 2 cut(s) 507, 627
BaeGI GKGCMC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 576
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 3 cut(s) 171, 328, 399
BccI CCATC 2 cut(s) 304, 515
BceAI ACGGC 1 cut(s) 189
BciT130I CCWGG 3 cut(s) 275, 296, 338
BcnI CCSGG 1 cut(s) 432
BfmI CTRYAG 2 cut(s) 90, 213
BfuAI ACCTGC 1 cut(s) 353
BglII AGATCT 1 cut(s) 456
BisI GCNGC 4 cut(s) 160, 202, 342, 413
BlsI GCNGC 4 cut(s) 161, 203, 343, 414
Bme1390I CCNGG 4 cut(s) 275, 296, 338, 432
Bme18I GGWCC 4 cut(s) 135, 298, 313, 502
BmgT120I GGNCC 5 cut(s) 135, 298, 313, 352, 502
BmiI GGNNCC 4 cut(s) 279, 353, 504, 624
BmrFI CCNGG 4 cut(s) 275, 296, 338, 432
BmsI GCATC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 596
BpuMI CCSGG 1 cut(s) 432
BsaJI CCNNGG 3 cut(s) 355, 399, 431
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse21I CCTNAGG 2 cut(s) 507, 627
Bse3DI GCAATG 2 cut(s) 255, 407
BseBI CCWGG 3 cut(s) 275, 296, 338
BseDI CCNNGG 3 cut(s) 355, 399, 431
BseGI GGATG 1 cut(s) 454
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMI GCAATG 2 cut(s) 255, 407
BseMII CTCAG 6 cut(s) 40, 281, 407, 474, 498, 582
BseRI GAGGAG 3 cut(s) 544, 653, 656
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 3 cut(s) 171, 328, 399
BseYI CCCAGC 1 cut(s) 381
Bsh1236I CGCG 1 cut(s) 250
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 1 cut(s) 354
BshNI GGYRCC 1 cut(s) 277
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 576
BsiSI CCGG 2 cut(s) 186, 431
BslFI GGGAC 2 cut(s) 28, 398
BslI CCNNNNNNNGG 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 28, 398
BsnI GGCC 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 476, 576
Bsp143I GATC 5 cut(s) 229, 368, 456, 550, 582
BspACI CCGC 4 cut(s) 157, 175, 202, 250
BspANI GGCC 1 cut(s) 354
BspCNI CTCAG 6 cut(s) 39, 282, 408, 473, 499, 583
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 4 cut(s) 279, 353, 504, 624
BspMI ACCTGC 1 cut(s) 353
BspPI GGATC 3 cut(s) 224, 376, 558
BspT107I GGYRCC 1 cut(s) 277
BsrBI CCGCTC 1 cut(s) 177
BsrDI GCAATG 2 cut(s) 255, 407
BssECI CCNNGG 3 cut(s) 355, 399, 431
BssMI GATC 5 cut(s) 229, 368, 456, 550, 582
Bst2UI CCWGG 3 cut(s) 275, 296, 338
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 3 cut(s) 59, 81, 370
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 8 cut(s) 26, 290, 416, 460, 507, 561, 591, 627
BstF5I GGATG 1 cut(s) 454
BstFNI CGCG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 5 cut(s) 232, 371, 459, 553, 585
BstMBI GATC 5 cut(s) 229, 368, 456, 550, 582
BstMCI CGRYCG 1 cut(s) 175
BstNI CCWGG 3 cut(s) 275, 296, 338
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 4 cut(s) 273, 294, 336, 430
BstSFI CTRYAG 2 cut(s) 90, 213
BstSLI GKGCMC 1 cut(s) 476
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 3 cut(s) 171, 328, 399
BstX2I RGATCY 2 cut(s) 456, 550
BstYI RGATCY 2 cut(s) 456, 550
Bsu36I CCTNAGG 2 cut(s) 507, 627
BsuRI GGCC 1 cut(s) 354
BtsCI GGATG 1 cut(s) 454
BtsIMutI CAGTG 1 cut(s) 468
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 1 cut(s) 558
CfoI GCGC 1 cut(s) 362
Cfr13I GGNCC 5 cut(s) 135, 298, 313, 352, 502
CsiI ACCWGGT 1 cut(s) 273
CviAII CATG 2 cut(s) 322, 427
DdeI CTNAG 8 cut(s) 26, 290, 416, 460, 507, 561, 591, 627
DpnI GATC 5 cut(s) 231, 370, 458, 552, 584
DpnII GATC 5 cut(s) 229, 368, 456, 550, 582
Eam1104I CTCTTC 3 cut(s) 59, 81, 370
EarI CTCTTC 3 cut(s) 59, 81, 370
Ecl136II GAGCTC 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 576
Eco47I GGWCC 4 cut(s) 135, 298, 313, 502
Eco53kI GAGCTC 1 cut(s) 574
Eco81I CCTNAGG 2 cut(s) 507, 627
EcoICRI GAGCTC 1 cut(s) 574
EcoO109I RGGNCCY 3 cut(s) 298, 352, 502
EcoRII CCWGG 3 cut(s) 273, 294, 336
EcoT38I GRGCYC 1 cut(s) 576
FaeI CATG 2 cut(s) 325, 430
FaiI YATR 7 cut(s) 215, 240, 323, 334, 348, 428, 570
FaqI GGGAC 2 cut(s) 28, 398
FatI CATG 2 cut(s) 321, 426
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 4 cut(s) 160, 202, 342, 413
FokI GGATG 1 cut(s) 461
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 4 cut(s) 160, 202, 342, 413
GlaI GCGC 1 cut(s) 361
GluI GCNGC 4 cut(s) 160, 202, 342, 413
GsaI CCCAGC 1 cut(s) 385
HaeIII GGCC 1 cut(s) 354
HapII CCGG 2 cut(s) 186, 431
HhaI GCGC 1 cut(s) 362
Hin1II CATG 2 cut(s) 325, 430
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 3 cut(s) 245, 328, 404
HpaII CCGG 2 cut(s) 186, 431
HphI GGTGA 3 cut(s) 524, 643, 676
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 4 cut(s) 29, 270, 291, 480
Hpy188III TCNNGA 1 cut(s) 372
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 3 cut(s) 149, 152, 173
HpyAV CCTTC 3 cut(s) 24, 124, 488
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 1 cut(s) 260
HpyF3I CTNAG 8 cut(s) 26, 290, 416, 460, 507, 561, 591, 627
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 2 cut(s) 325, 430
HspAI GCGC 1 cut(s) 360
Kzo9I GATC 5 cut(s) 229, 368, 456, 550, 582
LmnI GCTCC 4 cut(s) 116, 182, 252, 466
Lsp1109I GCAGC 3 cut(s) 171, 328, 399
LweI GCATC 1 cut(s) 44
MabI ACCWGGT 1 cut(s) 273
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 206
MalI GATC 5 cut(s) 231, 370, 458, 552, 584
MbiI CCGCTC 1 cut(s) 177
MboI GATC 5 cut(s) 229, 368, 456, 550, 582
MboII GAAGA 8 cut(s) 46, 68, 387, 466, 530, 610, 658, 661
MflI RGATCY 2 cut(s) 456, 550
MhlI GDGCHC 2 cut(s) 476, 576
MluCI AATT 2 cut(s) 317, 577
MlyI GAGTC 2 cut(s) 254, 398
MspI CCGG 2 cut(s) 186, 431
MspR9I CCNGG 4 cut(s) 275, 296, 338, 432
MvaI CCWGG 3 cut(s) 275, 296, 338
MvnI CGCG 1 cut(s) 250
NciI CCSGG 1 cut(s) 432
NdeII GATC 5 cut(s) 229, 368, 456, 550, 582
NlaIII CATG 2 cut(s) 325, 430
NlaIV GGNNCC 4 cut(s) 279, 353, 504, 624
NmeAIII GCCGAG 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 206
NspI RCATGY 1 cut(s) 430
PaqCI CACCTGC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 328
PflMI CCANNNNNTGG 1 cut(s) 322
PkrI GCNGC 4 cut(s) 161, 203, 343, 414
PleI GAGTC 2 cut(s) 253, 398
PpsI GAGTC 2 cut(s) 253, 398
PpuMI RGGWCCY 2 cut(s) 298, 502
Psp124BI GAGCTC 1 cut(s) 576
Psp5II RGGWCCY 2 cut(s) 298, 502
Psp6I CCWGG 3 cut(s) 273, 294, 336
PspFI CCCAGC 1 cut(s) 381
PspGI CCWGG 3 cut(s) 273, 294, 336
PspN4I GGNNCC 4 cut(s) 279, 353, 504, 624
PspPI GGNCC 5 cut(s) 135, 298, 313, 352, 502
PspPPI RGGWCCY 2 cut(s) 298, 502
PsuI RGATCY 2 cut(s) 456, 550
SacI GAGCTC 1 cut(s) 576
SalI GTCGAC 1 cut(s) 170
SatI GCNGC 4 cut(s) 160, 202, 342, 413
Sau3AI GATC 5 cut(s) 229, 368, 456, 550, 582
Sau96I GGNCC 5 cut(s) 135, 298, 313, 352, 502
SchI GAGTC 2 cut(s) 254, 398
ScrFI CCNGG 4 cut(s) 275, 296, 338, 432
SduI GDGCHC 2 cut(s) 476, 576
SexAI ACCWGGT 1 cut(s) 273
SfaNI GCATC 1 cut(s) 44
SfcI CTRYAG 2 cut(s) 90, 213
SinI GGWCC 4 cut(s) 135, 298, 313, 502
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 2 cut(s) 317, 577
SsiI CCGC 4 cut(s) 157, 175, 202, 250
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 4 cut(s) 273, 294, 336, 430
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 1 cut(s) 390
TaqI TCGA 2 cut(s) 171, 581
TasI AATT 2 cut(s) 317, 577
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 1 cut(s) 328
TscAI CASTG 1 cut(s) 468
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 3 cut(s) 159, 341, 412
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 2 cut(s) 310, 611
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 1 cut(s) 468
Van91I CCANNNNNTGG 1 cut(s) 322
VpaK11BI GGWCC 4 cut(s) 135, 298, 313, 502
XceI RCATGY 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.