Rorug05G0394700

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004776
Physical Location & Seq
Reverse (-)
54144336 .. 54145566
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0394700.1

Sequence Viewer

Length: 486 bp
ATGGTCTCAGAGAGCTGCGTGGATAGTCTACTCACAGAGATGGTCTCCATGTACTGCAATCGGTTATACACCCATAAGCCCGAGCTTGCTGCCAGTAGGATCCACGCTATTGGCTACCAGGTCGGCCACCAGCTCTCCGAGAGGTATACAATGGAGCGGCCTGGTTTACTGATCATCTCGATGCAATTAAGTTCATCTGCAAGGATTTCTGGTCTGAGCTTTTCAAGAAAGCAGATAGGCGACTTGAAGATCAATCACAGGGTAACTTCTAGAATCGATGTGAATTTGCATTCGTCCACTGAAAATGGAGACGTATCTCAAGAAAATTCTGAGGCCTCGGCTGAAAACAAGGCGGCACAAGTACCGACCATGCACCTCCATTTCTCTTGCAGGGTCATTAAAGGGGCTCTTCATAACTTGGGGATTACTTGCGCTGTTTCTGTGGACACATCCCATCTCCCTGCAGGTGAGTGTCTCTCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

161

Amino Acids

17.61

Weight (kDa)

6.74

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPP PF04051 8 - 154 2.3e-12 Transport protein particle (TRAPP) component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 455
Acc36I ACCTGC 1 cut(s) 455
AccBSI CCGCTC 1 cut(s) 157
AccI GTMKAC 2 cut(s) 28, 146
AciI CCGC 2 cut(s) 157, 353
AclWI GGATC 2 cut(s) 94, 107
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 2 cut(s) 283, 325
AfaI GTAC 2 cut(s) 53, 363
AgsI TTSAA 2 cut(s) 225, 247
AjnI CCWGG 2 cut(s) 117, 160
AluBI AGCT 4 cut(s) 15, 85, 133, 219
AluI AGCT 4 cut(s) 15, 85, 133, 219
Alw26I GTCTC 4 cut(s) 10, 49, 303, 479
AlwI GGATC 2 cut(s) 94, 107
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 3 cut(s) 124, 158, 333
ApeKI GCWGC 2 cut(s) 15, 89
ApoI RAATTY 2 cut(s) 283, 325
AspLEI GCGC 1 cut(s) 434
AsuHPI GGTGA 1 cut(s) 479
AvaI CYCGRG 1 cut(s) 80
BamHI GGATCC 1 cut(s) 99
BanII GRGCYC 1 cut(s) 409
BbvI GCAGC 2 cut(s) 2, 76
BccI CCATC 2 cut(s) 34, 462
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 2 cut(s) 119, 162
BclI TGATCA 1 cut(s) 171
BcoDI GTCTC 4 cut(s) 10, 49, 303, 479
BfaI CTAG 1 cut(s) 270
BfmI CTRYAG 1 cut(s) 462
BfuAI ACCTGC 1 cut(s) 455
BisI GCNGC 4 cut(s) 16, 90, 158, 354
BlsI GCNGC 4 cut(s) 17, 91, 159, 355
Bme1390I CCNGG 2 cut(s) 119, 162
BmeT110I CYCGRG 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 119, 162
BmsI GCATC 1 cut(s) 171
BplI GAGNNNNNCTC 3 cut(s) 29, 61, 461
BpuEI CTTGAG 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 276
BsaI GGTCTC 2 cut(s) 10, 49
BsaJI CCNNGG 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bse1I ACTGG 1 cut(s) 93
BseBI CCWGG 2 cut(s) 119, 162
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 449
BseMII CTCAG 3 cut(s) 21, 206, 321
BseNI ACTGG 1 cut(s) 93
BseXI GCAGC 2 cut(s) 2, 76
BshFI GGCC 3 cut(s) 126, 160, 335
BshVI ATCGAT 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 80
BsmAI GTCTC 4 cut(s) 10, 49, 303, 479
BsmBI CGTCTC 1 cut(s) 303
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 3 cut(s) 126, 160, 335
Bso31I GGTCTC 2 cut(s) 10, 49
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 409
Bsp143I GATC 3 cut(s) 99, 171, 249
BspACI CCGC 2 cut(s) 157, 353
BspANI GGCC 3 cut(s) 126, 160, 335
BspCNI CTCAG 3 cut(s) 20, 207, 322
BspDI ATCGAT 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 101
BspMAI CTGCAG 1 cut(s) 466
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 2 cut(s) 94, 107
BspQI GCTCTTC 1 cut(s) 414
BspTNI GGTCTC 2 cut(s) 10, 49
BsrBI CCGCTC 1 cut(s) 157
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 3 cut(s) 99, 171, 249
BssNAI GTATAC 1 cut(s) 147
Bst1107I GTATAC 1 cut(s) 147
Bst2UI CCWGG 2 cut(s) 119, 162
Bst6I CTCTTC 1 cut(s) 414
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 3 cut(s) 7, 215, 330
BstF5I GGATG 1 cut(s) 449
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 3 cut(s) 102, 174, 252
BstMAI GTCTC 4 cut(s) 10, 49, 303, 479
BstMBI GATC 3 cut(s) 99, 171, 249
BstNI CCWGG 2 cut(s) 119, 162
BstSCI CCNGG 2 cut(s) 117, 160
BstSFI CTRYAG 1 cut(s) 462
BstV1I GCAGC 2 cut(s) 2, 76
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 1 cut(s) 110
BstYI RGATCY 1 cut(s) 99
BstZ17I GTATAC 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 3 cut(s) 126, 160, 335
BsuTUI ATCGAT 1 cut(s) 276
BtsCI GGATG 1 cut(s) 449
BtsIMutI CAGTG 1 cut(s) 297
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 87
CfoI GCGC 1 cut(s) 434
ClaI ATCGAT 1 cut(s) 276
CsiI ACCWGGT 1 cut(s) 117
Csp6I GTAC 2 cut(s) 52, 362
CviAII CATG 2 cut(s) 49, 370
CviQI GTAC 2 cut(s) 52, 362
DdeI CTNAG 3 cut(s) 7, 215, 330
DpnI GATC 3 cut(s) 101, 173, 251
DpnII GATC 3 cut(s) 99, 171, 249
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 409
Eco31I GGTCTC 2 cut(s) 10, 49
Eco88I CYCGRG 1 cut(s) 80
EcoRII CCWGG 2 cut(s) 117, 160
EcoT38I GRGCYC 1 cut(s) 409
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 2 cut(s) 52, 373
FaiI YATR 6 cut(s) 50, 67, 75, 147, 371, 414
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FatI CATG 2 cut(s) 48, 369
FbaI TGATCA 1 cut(s) 171
FblI GTMKAC 2 cut(s) 28, 146
Fnu4HI GCNGC 4 cut(s) 16, 90, 158, 354
FokI GGATG 1 cut(s) 436
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 4 cut(s) 16, 90, 158, 354
FspBI CTAG 1 cut(s) 270
GlaI GCGC 1 cut(s) 433
GluI GCNGC 4 cut(s) 16, 90, 158, 354
HaeIII GGCC 3 cut(s) 126, 160, 335
HhaI GCGC 1 cut(s) 434
Hin1II CATG 2 cut(s) 52, 373
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HinfI GANTC 1 cut(s) 273
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 5 cut(s) 29, 147, 167, 297, 445
Hpy188I TCNGA 4 cut(s) 10, 139, 216, 331
Hpy188III TCNNGA 4 cut(s) 178, 225, 270, 320
Hpy8I GTNNAC 5 cut(s) 29, 147, 167, 297, 445
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 7 cut(s) 57, 184, 200, 289, 373, 390, 464
HpyF3I CTNAG 3 cut(s) 7, 215, 330
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 2 cut(s) 52, 373
HspAI GCGC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 3 cut(s) 99, 171, 249
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 154
Lsp1109I GCAGC 2 cut(s) 2, 76
LweI GCATC 1 cut(s) 171
MabI ACCWGGT 1 cut(s) 117
MaeI CTAG 1 cut(s) 270
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 262
MalI GATC 3 cut(s) 101, 173, 251
MbiI CCGCTC 1 cut(s) 157
MboI GATC 3 cut(s) 99, 171, 249
MboII GAAGA 2 cut(s) 259, 401
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 3 cut(s) 185, 283, 325
MnlI CCTC 4 cut(s) 135, 325, 346, 386
MseI TTAA 2 cut(s) 188, 399
MslI CAYNNNNRTG 2 cut(s) 38, 179
MspR9I CCNGG 2 cut(s) 119, 162
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 2 cut(s) 119, 162
NdeII GATC 3 cut(s) 99, 171, 249
NlaIII CATG 2 cut(s) 52, 373
NlaIV GGNNCC 1 cut(s) 101
NmeAIII GCCGAG 1 cut(s) 317
PaqCI CACCTGC 1 cut(s) 455
PceI AGGCCT 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 273
PkrI GCNGC 4 cut(s) 17, 91, 159, 355
Psp6I CCWGG 2 cut(s) 117, 160
PspGI CCWGG 2 cut(s) 117, 160
PspN4I GGNNCC 1 cut(s) 101
PstI CTGCAG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 2 cut(s) 53, 363
RsaNI GTAC 2 cut(s) 52, 362
RseI CAYNNNNRTG 2 cut(s) 38, 179
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 2 cut(s) 188, 399
SatI GCNGC 4 cut(s) 16, 90, 158, 354
Sau3AI GATC 3 cut(s) 99, 171, 249
SbfI CCTGCAGG 1 cut(s) 466
ScrFI CCNGG 2 cut(s) 119, 162
SdaI CCTGCAGG 1 cut(s) 466
SduI GDGCHC 1 cut(s) 409
SetI ASST 9 cut(s) 17, 87, 123, 135, 146, 221, 315, 378, 469
SexAI ACCWGGT 1 cut(s) 117
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 1 cut(s) 462
SmiMI CAYNNNNRTG 2 cut(s) 38, 179
SmlI CTYRAG 1 cut(s) 318
SmoI CTYRAG 1 cut(s) 318
Sse8387I CCTGCAGG 1 cut(s) 466
Sse9I AATT 3 cut(s) 185, 283, 325
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 2 cut(s) 157, 353
SspMI CTAG 1 cut(s) 270
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 2 cut(s) 117, 160
TaiI ACGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 179, 276
TasI AATT 3 cut(s) 185, 283, 325
TatI WGTACW 1 cut(s) 51
TauI GCSGC 2 cut(s) 160, 356
TfiI GAWTC 1 cut(s) 273
Tru1I TTAA 2 cut(s) 188, 399
Tru9I TTAA 2 cut(s) 188, 399
TscAI CASTG 1 cut(s) 304
TseI GCWGC 2 cut(s) 15, 89
TspDTI ATGAA 2 cut(s) 183, 401
TspRI CASTG 1 cut(s) 304
XapI RAATTY 2 cut(s) 283, 325
XbaI TCTAGA 1 cut(s) 269
XmiI GTMKAC 2 cut(s) 28, 146
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.