Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004776

Physical Location & Seq

Genomic Coordinates Forward (+)

32097833 .. 32098492

Gene Structure 660 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1253800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 660 bp

ATGGCTTCCTCACTTGCCCTCCGCCGCCTCCTCTCCGCGAACCGCTTCCCCAAGTCCTTCGCCATCTCCACTATCCGCCCACTCGCCATTACCCGCTCCCCCGCTTCCAAGCTCTTCAACACCAACGCTCTCCGCAGCTCCGACGATGACAACAATGAAAGCGACCTCAACTTCGACCGCCGCTCCGGCCGCCATTTCTCTCGTCGAGGCGACGATTTCTTCTCAGATGTGTTCGTCCCGTTCTCGCCGAGGCCCAACCTGAGCCACGTGCTGAACCTGATGGACCAGATCTCGGAGAATCCCATCCTCTCGGCGACACGTGGCGTCGGGGAGGCGGGGCTTCGCCTAGGCTGGGGTACGCGGGAGACGGAGGACGCTCTGCATCTCCGATTCGACATGCCTGGGCTCGGGAAGGAGGACGTGAAGATCTCGGTGGAGCACAACAACATCCTGAGCATCAAGGGAGAAGGCGGGAAGGAAGACAACGTCGGAGAGGAAGAGGCGCCGCCGCGGAGGTACAACACCGGGATCGGGCTGCCGGAGAAGATGTTCAAGACCGACAGCATCAAGGCGGAGATGAAGAACGGCGTGCTGAAGGTGGTTGTTCCGAAGTTGAAGGAAGAGGAGAAGGCCGACGTCTTCCACGTGAAGGTCGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

HSP20

Protein Analysis

220

Amino Acids

24.43

Weight (kDa)

7.0

Isoelectric Point (pI)

54.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP20	PF00011	121 - 218	5.8e-17	Hsp20/alpha crystallin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25200 AT5G51440
fragaria_vesca	FvH4_5g18720 FvH4_5g18720 FvH4_7g27540
malus_domestica	MD06G1060300.v1.1 MD07G1253800.v1.1
prunus_persica	Prupe.5G071200_v2.0.a1
pyrus_communis	pycom06g04980
rosa_chinensis	RchiOBHm_Chr1g0374411 RchiOBHm_Chr7g0203041
rosa_laevigata	RLG00000003557 RLG00000026740
rosa_multiflora	Rmu_co7998068.1_g000001 Rmu_sc0004999.1_g000002 Rmu_sc0006877.1_g000005
rosa_roxburghii	Rroxscaffold_3G00254100 Rroxscaffold_4G00283520
rosa_rugosa	Rorug05G0394700 Rorug07G0074200 Rorug07G0285900
rosa_samantha	Rh1AG393600 Rh1BG357900 Rh1DG388800 Rh7AG202800 Rh7BG202600 Rh7CG213600 Rh7DG209800
rosa_wichuraiana	Rw1G034130 Rw1G034250 Rw7G017710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	639
AccB1I	GGYRCC	1 cut(s)	502
AccBSI	CCGCTC	2 cut(s)	96, 183
AccII	CGCG	3 cut(s)	38, 361, 511
AclWI	GGATC	1 cut(s)	536
AcoI	YGGCCR	1 cut(s)	187
AcuI	CTGAAG	1 cut(s)	614
AcvI	CACGTG	3 cut(s)	268, 320, 646
AcyI	GRCGYC	3 cut(s)	324, 503, 636
AfaI	GTAC	2 cut(s)	358, 518
AfiI	CCNNNNNNNGG	5 cut(s)	292, 352, 407, 531, 649
AflIII	ACRYGT	1 cut(s)	317
AgsI	TTSAA	3 cut(s)	118, 553, 616
AjiI	CACGTC	1 cut(s)	421
AjnI	CCWGG	1 cut(s)	400
AloI	GAACNNNNNNTCC	2 cut(s)	533, 565
AluBI	AGCT	2 cut(s)	112, 138
AluI	AGCT	2 cut(s)	112, 138
Alw21I	GWGCWC	1 cut(s)	441
Alw26I	GTCTC	1 cut(s)	359
AlwI	GGATC	1 cut(s)	536
Ama87I	CYCGRG	1 cut(s)	407
AoxI	GGCC	3 cut(s)	187, 251, 630
ApeKI	GCWGC	2 cut(s)	135, 535
ArsI	GACNNNNNNTTYG	2 cut(s)	155, 187
Asp700I	GAANNNNTTC	2 cut(s)	44, 548
AspA2I	CCTAGG	1 cut(s)	346
AspLEI	GCGC	1 cut(s)	505
AspS9I	GGNCC	2 cut(s)	252, 283
AsuC2I	CCSGG	1 cut(s)	526
AvaI	CYCGRG	1 cut(s)	407
AvaII	GGWCC	1 cut(s)	283
AvrII	CCTAGG	1 cut(s)	346
BanI	GGYRCC	1 cut(s)	502
BanII	GRGCYC	1 cut(s)	408
BbrPI	CACGTG	3 cut(s)	268, 320, 646
BbsI	GAAGAC	2 cut(s)	486, 631
Bbv12I	GWGCWC	1 cut(s)	441
BbvI	GCAGC	2 cut(s)	147, 522
BccI	CCATC	3 cut(s)	71, 274, 311
BceAI	ACGGC	1 cut(s)	601
BciT130I	CCWGG	1 cut(s)	402
BcnI	CCSGG	1 cut(s)	526
BcoDI	GTCTC	1 cut(s)	359
BfaI	CTAG	1 cut(s)	347
BfoI	RGCGCY	1 cut(s)	506
BglI	GCCNNNNNGGC	1 cut(s)	186
BglII	AGATCT	2 cut(s)	288, 426
BisI	GCNGC	7 cut(s)	25, 136, 181, 190, 506, 509, 536
BlnI	CCTAGG	1 cut(s)	346
BlsI	GCNGC	7 cut(s)	26, 137, 182, 191, 507, 510, 537
Bme1390I	CCNGG	2 cut(s)	402, 526
Bme18I	GGWCC	1 cut(s)	283
BmeT110I	CYCGRG	1 cut(s)	407
BmgBI	CACGTC	1 cut(s)	421
BmgT120I	GGNCC	2 cut(s)	252, 283
BmiI	GGNNCC	1 cut(s)	504
BmrFI	CCNGG	2 cut(s)	402, 526
BmsI	GCATC	3 cut(s)	391, 465, 573
BpiI	GAAGAC	2 cut(s)	486, 631
Bpu10I	CCTNAGC	2 cut(s)	260, 452
BpuMI	CCSGG	1 cut(s)	526
BsaAI	YACGTR	3 cut(s)	268, 320, 646
BsaHI	GRCGYC	3 cut(s)	324, 503, 636
BsaJI	CCNNGG	4 cut(s)	248, 346, 401, 509
BsaXI	ACNNNNNCTCC	5 cut(s)	33, 167, 197, 533, 563
Bsc4I	CCNNNNNNNGG	5 cut(s)	292, 352, 407, 531, 649
BseBI	CCWGG	1 cut(s)	402
BseDI	CCNNGG	4 cut(s)	248, 346, 401, 509
BseGI	GGATG	2 cut(s)	303, 447
BseLI	CCNNNNNNNGG	5 cut(s)	292, 352, 407, 531, 649
BseMII	CTCAG	3 cut(s)	237, 251, 443
BseRI	GAGGAG	2 cut(s)	20, 638
BseX3I	CGGCCG	1 cut(s)	187
BseXI	GCAGC	2 cut(s)	147, 522
BseYI	CCCAGC	1 cut(s)	351
Bsh1236I	CGCG	3 cut(s)	38, 361, 511
Bsh1285I	CGRYCG	2 cut(s)	178, 190
BshFI	GGCC	3 cut(s)	189, 253, 632
BshNI	GGYRCC	1 cut(s)	502
BsiEI	CGRYCG	2 cut(s)	178, 190
BsiHKAI	GWGCWC	1 cut(s)	441
BsiHKCI	CYCGRG	1 cut(s)	407
BsiSI	CCGG	3 cut(s)	186, 525, 539
BslFI	GGGAC	1 cut(s)	221
BslI	CCNNNNNNNGG	5 cut(s)	292, 352, 407, 531, 649
BsmAI	GTCTC	1 cut(s)	359
BsmBI	CGTCTC	1 cut(s)	359
BsmFI	GGGAC	1 cut(s)	221
BsnI	GGCC	3 cut(s)	189, 253, 632
BsoBI	CYCGRG	1 cut(s)	407
Bsp1286I	GDGCHC	2 cut(s)	408, 441
Bsp143I	GATC	3 cut(s)	288, 426, 528
BspANI	GGCC	3 cut(s)	189, 253, 632
BspCNI	CTCAG	3 cut(s)	236, 252, 444
BspFNI	CGCG	3 cut(s)	38, 361, 511
BspLI	GGNNCC	1 cut(s)	504
BspPI	GGATC	1 cut(s)	536
BspQI	GCTCTTC	1 cut(s)	119
BspT107I	GGYRCC	1 cut(s)	502
BsrBI	CCGCTC	2 cut(s)	96, 183
BssECI	CCNNGG	4 cut(s)	248, 346, 401, 509
BssMI	GATC	3 cut(s)	288, 426, 528
BssNI	GRCGYC	3 cut(s)	324, 503, 636
BssT1I	CCWWGG	1 cut(s)	346
Bst2UI	CCWGG	1 cut(s)	402
Bst6I	CTCTTC	3 cut(s)	119, 492, 615
BstACI	GRCGYC	3 cut(s)	324, 503, 636
BstBAI	YACGTR	3 cut(s)	268, 320, 646
BstC8I	GCNNGC	1 cut(s)	590
BstDEI	CTNAG	3 cut(s)	223, 260, 452
BstDSI	CCRYGG	1 cut(s)	509
BstF5I	GGATG	2 cut(s)	303, 447
BstFNI	CGCG	3 cut(s)	38, 361, 511
BstH2I	RGCGCY	1 cut(s)	506
BstHHI	GCGC	1 cut(s)	505
BstKTI	GATC	3 cut(s)	291, 429, 531
BstMAI	GTCTC	1 cut(s)	359
BstMBI	GATC	3 cut(s)	288, 426, 528
BstMCI	CGRYCG	2 cut(s)	178, 190
BstMWI	GCNNNNNNNGC	2 cut(s)	186, 189
BstNI	CCWGG	1 cut(s)	402
BstNSI	RCATGY	1 cut(s)	400
BstSCI	CCNGG	2 cut(s)	400, 524
BstUI	CGCG	3 cut(s)	38, 361, 511
BstV1I	GCAGC	2 cut(s)	147, 522
BstV2I	GAAGAC	2 cut(s)	486, 631
BstX2I	RGATCY	2 cut(s)	288, 426
BstYI	RGATCY	2 cut(s)	288, 426
BstZI	CGGCCG	1 cut(s)	187
BsuRI	GGCC	3 cut(s)	189, 253, 632
BtgI	CCRYGG	1 cut(s)	509
BtrI	CACGTC	1 cut(s)	421
BtsCI	GGATG	2 cut(s)	303, 447
Cac8I	GCNNGC	1 cut(s)	590
CfoI	GCGC	1 cut(s)	505
Cfr13I	GGNCC	2 cut(s)	252, 283
Cfr42I	CCGCGG	1 cut(s)	512
CseI	GACGC	2 cut(s)	313, 383
Csp6I	GTAC	2 cut(s)	357, 517
CviAII	CATG	1 cut(s)	397
CviQI	GTAC	2 cut(s)	357, 517
DdeI	CTNAG	3 cut(s)	223, 260, 452
DinI	GGCGCC	1 cut(s)	504
DpnI	GATC	3 cut(s)	290, 428, 530
DpnII	GATC	3 cut(s)	288, 426, 528
EaeI	YGGCCR	1 cut(s)	187
EagI	CGGCCG	1 cut(s)	187
Eam1104I	CTCTTC	3 cut(s)	119, 492, 615
EarI	CTCTTC	3 cut(s)	119, 492, 615
EciI	GGCGGA	3 cut(s)	11, 65, 587
EclXI	CGGCCG	1 cut(s)	187
Eco130I	CCWWGG	1 cut(s)	346
Eco24I	GRGCYC	1 cut(s)	408
Eco47I	GGWCC	1 cut(s)	283
Eco52I	CGGCCG	1 cut(s)	187
Eco57I	CTGAAG	1 cut(s)	614
Eco72I	CACGTG	3 cut(s)	268, 320, 646
Eco88I	CYCGRG	1 cut(s)	407
EcoRII	CCWGG	1 cut(s)	400
EcoT14I	CCWWGG	1 cut(s)	346
EcoT38I	GRGCYC	1 cut(s)	408
EgeI	GGCGCC	1 cut(s)	504
EheI	GGCGCC	1 cut(s)	504
ErhI	CCWWGG	1 cut(s)	346
Esp3I	CGTCTC	1 cut(s)	359
FaeI	CATG	1 cut(s)	400
FaiI	YATR	1 cut(s)	398
FaqI	GGGAC	1 cut(s)	221
FatI	CATG	1 cut(s)	396
FauI	CCCGC	5 cut(s)	101, 109, 328, 354, 464
Fnu4HI	GCNGC	7 cut(s)	25, 136, 181, 190, 506, 509, 536
FokI	GGATG	2 cut(s)	290, 434
FriOI	GRGCYC	1 cut(s)	408
Fsp4HI	GCNGC	7 cut(s)	25, 136, 181, 190, 506, 509, 536
FspBI	CTAG	1 cut(s)	347
GlaI	GCGC	1 cut(s)	504
GluI	GCNGC	7 cut(s)	25, 136, 181, 190, 506, 509, 536
GsaI	CCCAGC	1 cut(s)	355
HaeII	RGCGCY	1 cut(s)	506
HaeIII	GGCC	3 cut(s)	189, 253, 632
HapII	CCGG	3 cut(s)	186, 525, 539
HgaI	GACGC	2 cut(s)	313, 383
HhaI	GCGC	1 cut(s)	505
Hin1I	GRCGYC	3 cut(s)	324, 503, 636
Hin1II	CATG	1 cut(s)	400
Hin6I	GCGC	1 cut(s)	503
HinP1I	GCGC	1 cut(s)	503
HinfI	GANTC	2 cut(s)	298, 390
HpaII	CCGG	3 cut(s)	186, 525, 539
Hpy188I	TCNGA	6 cut(s)	142, 226, 295, 389, 491, 609
Hpy188III	TCNNGA	3 cut(s)	409, 451, 553
Hpy99I	CGWCG	6 cut(s)	146, 207, 215, 329, 491, 638
HpyAV	CCTTC	8 cut(s)	67, 406, 461, 469, 589, 610, 622, 643
HpyCH4IV	ACGT	6 cut(s)	267, 319, 420, 486, 636, 645
HpyCH4V	TGCA	1 cut(s)	382
HpyF10VI	GCNNNNNNNGC	2 cut(s)	186, 189
HpyF3I	CTNAG	3 cut(s)	223, 260, 452
HpySE526I	ACGT	6 cut(s)	267, 319, 420, 486, 636, 645
Hsp92I	GRCGYC	3 cut(s)	324, 503, 636
Hsp92II	CATG	1 cut(s)	400
HspAI	GCGC	1 cut(s)	503
KasI	GGCGCC	1 cut(s)	502
KspI	CCGCGG	1 cut(s)	512
Kzo9I	GATC	3 cut(s)	288, 426, 528
LguI	GCTCTTC	1 cut(s)	119
LmnI	GCTCC	4 cut(s)	101, 143, 188, 436
Lsp1109I	GCAGC	2 cut(s)	147, 522
LweI	GCATC	3 cut(s)	391, 465, 573
MaeI	CTAG	1 cut(s)	347
MaeII	ACGT	6 cut(s)	267, 319, 420, 486, 636, 645
MalI	GATC	3 cut(s)	290, 428, 530
MbiI	CCGCTC	2 cut(s)	96, 183
MboI	GATC	3 cut(s)	288, 426, 528
MboII	GAAGA	9 cut(s)	106, 211, 436, 491, 509, 556, 592, 631, 632
MflI	RGATCY	2 cut(s)	288, 426
MhlI	GDGCHC	2 cut(s)	408, 441
Mly113I	GGCGCC	1 cut(s)	503
MmeI	TCCRAC	2 cut(s)	165, 469
MroXI	GAANNNNTTC	2 cut(s)	44, 548
MspA1I	CMGCKG	1 cut(s)	511
MspI	CCGG	3 cut(s)	186, 525, 539
MspR9I	CCNGG	2 cut(s)	402, 526
MvaI	CCWGG	1 cut(s)	402
MvnI	CGCG	3 cut(s)	38, 361, 511
MwoI	GCNNNNNNNGC	2 cut(s)	186, 189
NarI	GGCGCC	1 cut(s)	503
NciI	CCSGG	1 cut(s)	526
NdeII	GATC	3 cut(s)	288, 426, 528
NlaIII	CATG	1 cut(s)	400
NlaIV	GGNNCC	1 cut(s)	504
NmeAIII	GCCGAG	2 cut(s)	273, 290
NspI	RCATGY	1 cut(s)	400
PciSI	GCTCTTC	1 cut(s)	119
PcsI	WCGNNNNNNNCGW	2 cut(s)	642, 651
PdmI	GAANNNNTTC	2 cut(s)	44, 548
PfeI	GAWTC	2 cut(s)	298, 390
PflFI	GACNNNGTC	1 cut(s)	485
PkrI	GCNGC	7 cut(s)	26, 137, 182, 191, 507, 510, 537
PluTI	GGCGCC	1 cut(s)	506
PmaCI	CACGTG	3 cut(s)	268, 320, 646
PmlI	CACGTG	3 cut(s)	268, 320, 646
Ppu21I	YACGTR	3 cut(s)	268, 320, 646
Psp6I	CCWGG	1 cut(s)	400
PspCI	CACGTG	3 cut(s)	268, 320, 646
PspFI	CCCAGC	1 cut(s)	351
PspGI	CCWGG	1 cut(s)	400
PspN4I	GGNNCC	1 cut(s)	504
PspPI	GGNCC	2 cut(s)	252, 283
PsuI	RGATCY	2 cut(s)	288, 426
PsyI	GACNNNGTC	1 cut(s)	485
RsaI	GTAC	2 cut(s)	358, 518
RsaNI	GTAC	2 cut(s)	357, 517
SacII	CCGCGG	1 cut(s)	512
SapI	GCTCTTC	1 cut(s)	119
SatI	GCNGC	7 cut(s)	25, 136, 181, 190, 506, 509, 536
Sau3AI	GATC	3 cut(s)	288, 426, 528
Sau96I	GGNCC	2 cut(s)	252, 283
ScrFI	CCNGG	2 cut(s)	402, 526
SduI	GDGCHC	2 cut(s)	408, 441
SfaNI	GCATC	3 cut(s)	391, 465, 573
SfoI	GGCGCC	1 cut(s)	504
Sfr303I	CCGCGG	1 cut(s)	512
SgrBI	CCGCGG	1 cut(s)	512
SinI	GGWCC	1 cut(s)	283
SspDI	GGCGCC	1 cut(s)	502
SspMI	CTAG	1 cut(s)	347
StyD4I	CCNGG	2 cut(s)	400, 524
StyI	CCWWGG	1 cut(s)	346
TaiI	ACGT	6 cut(s)	270, 322, 423, 489, 639, 648
TaqI	TCGA	4 cut(s)	174, 205, 393, 654
TaqII	GACCGA	1 cut(s)	572
TauI	GCSGC	5 cut(s)	27, 183, 192, 508, 511
TfiI	GAWTC	2 cut(s)	298, 390
TseI	GCWGC	2 cut(s)	135, 535
TspDTI	ATGAA	2 cut(s)	171, 593
TspGWI	ACGGA	1 cut(s)	383
Tth111I	GACNNNGTC	1 cut(s)	485
VpaK11BI	GGWCC	1 cut(s)	283
XceI	RCATGY	1 cut(s)	400
XmaJI	CCTAGG	1 cut(s)	346
XmnI	GAANNNNTTC	2 cut(s)	44, 548
XspI	CTAG	1 cut(s)	347
ZraI	GACGTC	1 cut(s)	637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.