pycom06g04980

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004776
Physical Location & Seq
Forward (+)
6681497 .. 6682625
1129 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g04980.1

Sequence Viewer

Length: 849 bp
ATGGAACCTTCTCCAATCCTCTGTTTTCCCCTCCTCCACGGTTCCCCTGTGATTATCACATCGCATGTGTCTCCTCTATATAAAACCCCTTCACTTCGATCCGCCTTCAAACCCAACAAGTTCAAGTCCAAATTTCCATTCACAAACCGATCAAAAACCCCAAATCAAAGCATCGTTAATCTCTTTTCTTGTAGAACAAAAATGGCTGCGTCAATGTCAATCCTTCTCAAAAGGGCCGCAGTACCAGCACCAACCATCCTCTCCAAGCTCTCCAACCCCATCCGTTCTGCCTCTGTATCTCCCCTCGTTTCTCGGTCTTTCAACACCAACAGCCAAGTCACCAACTACGATCAGGATGAAAGCTCAGTCCCCGTCGGTCGCGGCACCGCAGACATGTCTCCCTCCAGCCGCCGTGACCTCGGTTCTCCCTTCTTCTCAGATGTGTTCGATCCGTTTTCTCCGACGAGGAGCTTGAGCCAGGTTCTGAACATGGTGGACCAGTTCATGGACAACCCATTTCTGGCAGGGCCACGGGGAGGATCGAGAAGGGGATGGGACGTGAAGGAGAACGAGGAGGCTCTGTTTCTACGAATGGACATGCCGGGACTGGACAAGGAAGACGTGAAGATCTCGGTGGAGCAGAACACGCTGGTAGTAAAAGGGGAGGGGAAGGACTTGGAGGATGAAGACGGCGGAGCGAGGAGATTCTCGAGCAGACTGGAACTGCCTCCGAATCTGTACAAGATCGATTCGATTAGGGCAGAGATGAAGAATGGAGTTCTGAAGCTGGTGATTCCGAAGGTGAAGGAGGAAGAGAGAAAGGATGTGTTTGAGGTTAAGGTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

283

Amino Acids

31.38

Weight (kDa)

9.26

Isoelectric Point (pI)

65.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 186 - 281 3.6e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 505
AccII CGCG 1 cut(s) 381
AciI CCGC 6 cut(s) 102, 237, 381, 387, 409, 693
AclWI GGATC 3 cut(s) 93, 443, 547
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 1 cut(s) 803
AfaI GTAC 2 cut(s) 243, 740
AfiI CCNNNNNNNGG 3 cut(s) 505, 520, 536
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 3 cut(s) 109, 124, 322
AjiI CACGTC 2 cut(s) 559, 622
AjnI CCWGG 1 cut(s) 477
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 4 cut(s) 268, 363, 471, 787
AluI AGCT 4 cut(s) 268, 363, 471, 787
Alw26I GTCTC 2 cut(s) 75, 402
AlwI GGATC 3 cut(s) 93, 443, 547
AlwNI CAGNNNCTG 1 cut(s) 484
Ama87I CYCGRG 1 cut(s) 709
AoxI GGCC 2 cut(s) 234, 527
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 234, 496, 527
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 3 cut(s) 331, 802, 814
AvaI CYCGRG 1 cut(s) 709
AvaII GGWCC 1 cut(s) 496
BanI GGYRCC 1 cut(s) 383
BbsI GAAGAC 2 cut(s) 624, 693
BbvI GCAGC 1 cut(s) 193
BccI CCATC 3 cut(s) 263, 287, 546
BceAI ACGGC 2 cut(s) 396, 706
BciT130I CCWGG 1 cut(s) 479
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 2 cut(s) 75, 402
BglII AGATCT 1 cut(s) 627
BisI GCNGC 4 cut(s) 207, 237, 382, 409
BlsI GCNGC 4 cut(s) 208, 238, 383, 410
Bme1390I CCNGG 2 cut(s) 479, 603
Bme18I GGWCC 1 cut(s) 496
BmeT110I CYCGRG 1 cut(s) 709
BmgBI CACGTC 2 cut(s) 559, 622
BmgT120I GGNCC 3 cut(s) 234, 496, 527
BmiI GGNNCC 3 cut(s) 6, 43, 385
BmrFI CCNGG 2 cut(s) 479, 603
BmsI GCATC 1 cut(s) 180
BpiI GAAGAC 2 cut(s) 624, 693
BpmI CTGGAG 1 cut(s) 388
BpuEI CTTGAG 1 cut(s) 493
BpuMI CCSGG 1 cut(s) 603
Bsa29I ATCGAT 1 cut(s) 747
BsaJI CCNNGG 3 cut(s) 37, 418, 530
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bsc4I CCNNNNNNNGG 3 cut(s) 505, 520, 536
Bse1I ACTGG 3 cut(s) 499, 612, 723
BseBI CCWGG 1 cut(s) 479
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 3 cut(s) 37, 418, 530
BseGI GGATG 6 cut(s) 255, 279, 361, 557, 688, 829
BseLI CCNNNNNNNGG 3 cut(s) 505, 520, 536
BseMII CTCAG 2 cut(s) 378, 450
BseNI ACTGG 3 cut(s) 499, 612, 723
BseRI GAGGAG 5 cut(s) 23, 63, 481, 587, 715
BseXI GCAGC 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 381
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 2 cut(s) 236, 529
BshNI GGYRCC 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 747
BsiEI CGRYCG 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 709
BsiSI CCGG 1 cut(s) 602
BslFI GGGAC 3 cut(s) 353, 569, 618
BslI CCNNNNNNNGG 3 cut(s) 505, 520, 536
BsmAI GTCTC 2 cut(s) 75, 402
BsmFI GGGAC 3 cut(s) 353, 569, 618
BsnI GGCC 2 cut(s) 236, 529
BsoBI CYCGRG 1 cut(s) 709
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 7 cut(s) 98, 149, 349, 448, 539, 627, 744
BspACI CCGC 6 cut(s) 102, 237, 381, 387, 409, 693
BspANI GGCC 2 cut(s) 236, 529
BspCNI CTCAG 2 cut(s) 377, 449
BspDI ATCGAT 1 cut(s) 747
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 3 cut(s) 6, 43, 385
BspPI GGATC 3 cut(s) 93, 443, 547
BspT107I GGYRCC 1 cut(s) 383
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 3 cut(s) 499, 612, 723
BssECI CCNNGG 3 cut(s) 37, 418, 530
BssMI GATC 7 cut(s) 98, 149, 349, 448, 539, 627, 744
Bst2UI CCWGG 1 cut(s) 479
Bst4CI ACNGT 1 cut(s) 41
Bst6I CTCTTC 1 cut(s) 807
BstAUI TGTACA 1 cut(s) 738
BstDEI CTNAG 2 cut(s) 364, 436
BstDSI CCRYGG 2 cut(s) 37, 530
BstF5I GGATG 6 cut(s) 255, 279, 361, 557, 688, 829
BstFNI CGCG 1 cut(s) 381
BstKTI GATC 7 cut(s) 101, 152, 352, 451, 542, 630, 747
BstMAI GTCTC 2 cut(s) 75, 402
BstMBI GATC 7 cut(s) 98, 149, 349, 448, 539, 627, 744
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 2 cut(s) 245, 646
BstNI CCWGG 1 cut(s) 479
BstNSI RCATGY 3 cut(s) 68, 397, 601
BstSCI CCNGG 2 cut(s) 477, 601
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 2 cut(s) 624, 693
BstX2I RGATCY 1 cut(s) 627
BstYI RGATCY 1 cut(s) 627
Bsu15I ATCGAT 1 cut(s) 747
BsuRI GGCC 2 cut(s) 236, 529
BsuTUI ATCGAT 1 cut(s) 747
BtgI CCRYGG 2 cut(s) 37, 530
BtgZI GCGATG 1 cut(s) 45
BtrI CACGTC 2 cut(s) 559, 622
BtsCI GGATG 6 cut(s) 255, 279, 361, 557, 688, 829
CaiI CAGNNNCTG 1 cut(s) 484
Cfr13I GGNCC 3 cut(s) 234, 496, 527
ClaI ATCGAT 1 cut(s) 747
CseI GACGC 1 cut(s) 198
Csp6I GTAC 2 cut(s) 242, 739
CviAII CATG 5 cut(s) 65, 394, 490, 505, 598
CviQI GTAC 2 cut(s) 242, 739
DdeI CTNAG 2 cut(s) 364, 436
DpnI GATC 7 cut(s) 100, 151, 351, 450, 541, 629, 746
DpnII GATC 7 cut(s) 98, 149, 349, 448, 539, 627, 744
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
EciI GGCGGA 2 cut(s) 91, 708
Eco47I GGWCC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 803
Eco88I CYCGRG 1 cut(s) 709
EcoRII CCWGG 1 cut(s) 477
FaeI CATG 5 cut(s) 68, 397, 493, 508, 601
FaiI YATR 7 cut(s) 66, 79, 81, 395, 491, 506, 599
FaqI GGGAC 3 cut(s) 353, 569, 618
FatI CATG 5 cut(s) 64, 393, 489, 504, 597
Fnu4HI GCNGC 4 cut(s) 207, 237, 382, 409
FokI GGATG 6 cut(s) 242, 266, 368, 564, 695, 836
Fsp4HI GCNGC 4 cut(s) 207, 237, 382, 409
GluI GCNGC 4 cut(s) 207, 237, 382, 409
GsuI CTGGAG 1 cut(s) 388
HaeIII GGCC 2 cut(s) 236, 529
HapII CCGG 1 cut(s) 602
HgaI GACGC 1 cut(s) 198
Hin1II CATG 5 cut(s) 68, 397, 493, 508, 601
HinfI GANTC 4 cut(s) 705, 733, 749, 793
HpaII CCGG 1 cut(s) 602
HphI GGTGA 3 cut(s) 331, 802, 814
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 6 cut(s) 439, 462, 486, 732, 783, 798
Hpy188III TCNNGA 3 cut(s) 353, 543, 709
Hpy8I GTNNAC 1 cut(s) 496
Hpy99I CGWCG 2 cut(s) 377, 466
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4IV ACGT 2 cut(s) 558, 621
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 646
HpyF3I CTNAG 2 cut(s) 364, 436
HpySE526I ACGT 2 cut(s) 558, 621
Hsp92II CATG 5 cut(s) 68, 397, 493, 508, 601
Kzo9I GATC 7 cut(s) 98, 149, 349, 448, 539, 627, 744
LmnI GCTCC 3 cut(s) 468, 637, 695
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 1 cut(s) 180
MaeII ACGT 2 cut(s) 558, 621
MaeIII GTNAC 2 cut(s) 337, 413
MalI GATC 7 cut(s) 100, 151, 351, 450, 541, 629, 746
MboI GATC 7 cut(s) 98, 149, 349, 448, 539, 627, 744
MboII GAAGA 6 cut(s) 424, 629, 637, 698, 781, 824
MflI RGATCY 1 cut(s) 627
MluCI AATT 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 297, 485
MseI TTAA 2 cut(s) 177, 837
MspI CCGG 1 cut(s) 602
MspR9I CCNGG 2 cut(s) 479, 603
MvaI CCWGG 1 cut(s) 479
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 2 cut(s) 245, 646
NciI CCSGG 1 cut(s) 603
NdeII GATC 7 cut(s) 98, 149, 349, 448, 539, 627, 744
NlaIII CATG 5 cut(s) 68, 397, 493, 508, 601
NlaIV GGNNCC 3 cut(s) 6, 43, 385
NmuCI GTSAC 2 cut(s) 337, 413
NspI RCATGY 3 cut(s) 68, 397, 601
PaeR7I CTCGAG 1 cut(s) 709
PciI ACATGT 1 cut(s) 393
PfeI GAWTC 4 cut(s) 705, 733, 749, 793
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 4 cut(s) 208, 238, 383, 410
PscI ACATGT 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 3 cut(s) 6, 43, 385
PspPI GGNCC 3 cut(s) 234, 496, 527
PstNI CAGNNNCTG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 627
RsaI GTAC 2 cut(s) 243, 740
RsaNI GTAC 2 cut(s) 242, 739
SaqAI TTAA 2 cut(s) 177, 837
SatI GCNGC 4 cut(s) 207, 237, 382, 409
Sau3AI GATC 7 cut(s) 98, 149, 349, 448, 539, 627, 744
Sau96I GGNCC 3 cut(s) 234, 496, 527
ScrFI CCNGG 2 cut(s) 479, 603
SfaNI GCATC 1 cut(s) 180
Sfr274I CTCGAG 1 cut(s) 709
SinI GGWCC 1 cut(s) 496
SlaI CTCGAG 1 cut(s) 709
SmlI CTYRAG 2 cut(s) 472, 709
SmoI CTYRAG 2 cut(s) 472, 709
Sse9I AATT 1 cut(s) 131
SsiI CCGC 6 cut(s) 102, 237, 381, 387, 409, 693
StyD4I CCNGG 2 cut(s) 477, 601
TaaI ACNGT 1 cut(s) 41
TaiI ACGT 2 cut(s) 561, 624
TaqI TCGA 6 cut(s) 97, 447, 542, 710, 747, 752
TaqII GACCGA 2 cut(s) 303, 365
TasI AATT 1 cut(s) 131
TatI WGTACW 1 cut(s) 738
TauI GCSGC 3 cut(s) 239, 384, 411
TfiI GAWTC 4 cut(s) 705, 733, 749, 793
Tru1I TTAA 2 cut(s) 177, 837
Tru9I TTAA 2 cut(s) 177, 837
TseFI GTSAC 2 cut(s) 337, 413
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 2 cut(s) 337, 413
TspDTI ATGAA 4 cut(s) 372, 493, 699, 782
TspGWI ACGGA 2 cut(s) 272, 441
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 496
XapI RAATTY 1 cut(s) 131
XceI RCATGY 3 cut(s) 68, 397, 601
XhoI CTCGAG 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.