MD06G1060300.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004776
Physical Location & Seq
Forward (+)
9340096 .. 9341581
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1060300.v1.1.491

Sequence Viewer

Length: 789 bp
ATGCGTCTCCTCTATATAAAACCCCTTCCCCTTAAACCCCCCTTCCAACCCAACAAGTTCAAATCCAAATTTCCATTCACAAACCGATCAAAAACGCCAAATCAAAGCCGCTTCAATCTCTTTTCTTGTAGAACAAAAATGGCTTCGTCAATGTCAATCCTTCTCAAAAGGGCCGCAGCACCCGCACCAACCATCCTCTCCAAGCTCTCCAACCCCATCCGTTCTGCTTCTGTATCTCCCCTCGTTTCTCGGTCATTCAACACCAATAGCCAAGTCACCAGCTACGATCAGGATGAAAGCTCAGTCCCCGTTGGTCGCGGCACCGCCGACAGGTCTCCCTCCCGCCGCCGTGACCTCGGTTCTCCCTTCTTCTCAGGTAATGTGTTCGATCCGTTTTCTCCGACGAGGAGCTTGAGCCAGGTTCTGAACATGGTGGACCAGTTCATGGACAACCCATTTCTGGCAGGGCTACAGGGAGGATCGAGAAGGGGATGGGACGTGAAGGAGAACGAGGAGGCTCTGTTTCTGCGAATGGACATGCCGGGACTGGACAAGGAAGACGTGAAGATCTCGGTGCAGCAGAACATGCTGGTAGTGAAAGGGGAGGGGAAGGACTTGGAGGATGAAGACGGCGGAGCGAGGAGGTTCTCGAGCAGACTGGAACTGCCTCCGAATCTGTACAAGATCGATTCGATTAGGGCTGAGATGAAGAATGGAGTTCTGAAGCTGGTGATTCCGAAGGCGAAGGAGGAAGAGAGAAAGGATGTGTTTGAGGTTAAGGTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

263

Amino Acids

29.46

Weight (kDa)

9.85

Isoelectric Point (pI)

65.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 166 - 262 9.2e-19 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 320
AccB7I CCANNNNNTGG 1 cut(s) 445
AccII CGCG 1 cut(s) 318
AciI CCGC 8 cut(s) 109, 174, 183, 318, 324, 343, 346, 633
AclWI GGATC 2 cut(s) 383, 487
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 743
AfaI GTAC 1 cut(s) 680
AfiI CCNNNNNNNGG 3 cut(s) 330, 445, 460
AgsI TTSAA 3 cut(s) 61, 115, 259
AjiI CACGTC 2 cut(s) 499, 562
AjnI CCWGG 1 cut(s) 417
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AluBI AGCT 5 cut(s) 205, 282, 300, 411, 727
AluI AGCT 5 cut(s) 205, 282, 300, 411, 727
Alw26I GTCTC 2 cut(s) 11, 339
AlwI GGATC 2 cut(s) 383, 487
AlwNI CAGNNNCTG 1 cut(s) 424
Ama87I CYCGRG 1 cut(s) 649
AoxI GGCC 1 cut(s) 171
ApeKI GCWGC 2 cut(s) 176, 577
ApoI RAATTY 1 cut(s) 68
AspS9I GGNCC 2 cut(s) 171, 436
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 268, 742
AvaI CYCGRG 1 cut(s) 649
AvaII GGWCC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 320
BbsI GAAGAC 2 cut(s) 564, 633
BbvI GCAGC 2 cut(s) 188, 589
BccI CCATC 3 cut(s) 200, 224, 486
BceAI ACGGC 2 cut(s) 333, 646
BciT130I CCWGG 1 cut(s) 419
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 11, 339
BfmI CTRYAG 1 cut(s) 470
BglII AGATCT 1 cut(s) 567
BisI GCNGC 6 cut(s) 109, 174, 177, 319, 346, 578
BlsI GCNGC 6 cut(s) 110, 175, 178, 320, 347, 579
Bme1390I CCNGG 2 cut(s) 419, 543
Bme18I GGWCC 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 649
BmgBI CACGTC 2 cut(s) 499, 562
BmgT120I GGNCC 2 cut(s) 171, 436
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 2 cut(s) 419, 543
BpiI GAAGAC 2 cut(s) 564, 633
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 687
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 1 cut(s) 355
BsaXI ACNNNNNCTCC 2 cut(s) 506, 536
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 445, 460
Bse1I ACTGG 3 cut(s) 439, 552, 663
BseBI CCWGG 1 cut(s) 419
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 1 cut(s) 355
BseGI GGATG 6 cut(s) 192, 216, 298, 497, 628, 769
BseLI CCNNNNNNNGG 3 cut(s) 330, 445, 460
BseMII CTCAG 3 cut(s) 315, 387, 693
BseNI ACTGG 3 cut(s) 439, 552, 663
BseRI GAGGAG 3 cut(s) 421, 527, 655
BseXI GCAGC 2 cut(s) 188, 589
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 1 cut(s) 318
BshFI GGCC 1 cut(s) 173
BshNI GGYRCC 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 687
BsiHKCI CYCGRG 1 cut(s) 649
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 3 cut(s) 290, 509, 558
BslI CCNNNNNNNGG 3 cut(s) 330, 445, 460
BsmAI GTCTC 2 cut(s) 11, 339
BsmBI CGTCTC 1 cut(s) 11
BsmFI GGGAC 3 cut(s) 290, 509, 558
BsnI GGCC 1 cut(s) 173
Bso31I GGTCTC 1 cut(s) 339
BsoBI CYCGRG 1 cut(s) 649
Bsp1407I TGTACA 1 cut(s) 678
Bsp143I GATC 6 cut(s) 86, 286, 388, 479, 567, 684
BspACI CCGC 8 cut(s) 109, 174, 183, 318, 324, 343, 346, 633
BspANI GGCC 1 cut(s) 173
BspCNI CTCAG 3 cut(s) 314, 386, 694
BspDI ATCGAT 1 cut(s) 687
BspFNI CGCG 1 cut(s) 318
BspLI GGNNCC 1 cut(s) 322
BspPI GGATC 2 cut(s) 383, 487
BspT107I GGYRCC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 339
BsrGI TGTACA 1 cut(s) 678
BsrI ACTGG 3 cut(s) 439, 552, 663
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 6 cut(s) 86, 286, 388, 479, 567, 684
Bst2UI CCWGG 1 cut(s) 419
Bst6I CTCTTC 1 cut(s) 747
BstAPI GCANNNNNTGC 1 cut(s) 586
BstAUI TGTACA 1 cut(s) 678
BstDEI CTNAG 3 cut(s) 301, 373, 702
BstF5I GGATG 6 cut(s) 192, 216, 298, 497, 628, 769
BstFNI CGCG 1 cut(s) 318
BstKTI GATC 6 cut(s) 89, 289, 391, 482, 570, 687
BstMAI GTCTC 2 cut(s) 11, 339
BstMBI GATC 6 cut(s) 86, 286, 388, 479, 567, 684
BstMWI GCNNNNNNNGC 2 cut(s) 182, 586
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 2 cut(s) 541, 589
BstSCI CCNGG 2 cut(s) 417, 541
BstSFI CTRYAG 1 cut(s) 470
BstUI CGCG 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 188, 589
BstV2I GAAGAC 2 cut(s) 564, 633
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
Bsu15I ATCGAT 1 cut(s) 687
BsuRI GGCC 1 cut(s) 173
BsuTUI ATCGAT 1 cut(s) 687
BtrI CACGTC 2 cut(s) 499, 562
BtsCI GGATG 6 cut(s) 192, 216, 298, 497, 628, 769
CaiI CAGNNNCTG 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 171, 436
ClaI ATCGAT 1 cut(s) 687
Csp6I GTAC 1 cut(s) 679
CviAII CATG 4 cut(s) 430, 445, 538, 586
CviQI GTAC 1 cut(s) 679
DdeI CTNAG 3 cut(s) 301, 373, 702
DpnI GATC 6 cut(s) 88, 288, 390, 481, 569, 686
DpnII GATC 6 cut(s) 86, 286, 388, 479, 567, 684
Eam1104I CTCTTC 1 cut(s) 747
EarI CTCTTC 1 cut(s) 747
EciI GGCGGA 1 cut(s) 648
Eco31I GGTCTC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 743
Eco88I CYCGRG 1 cut(s) 649
EcoRII CCWGG 1 cut(s) 417
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 4 cut(s) 433, 448, 541, 589
FaiI YATR 6 cut(s) 15, 17, 431, 446, 539, 587
FaqI GGGAC 3 cut(s) 290, 509, 558
FatI CATG 4 cut(s) 429, 444, 537, 585
FauI CCCGC 2 cut(s) 190, 350
Fnu4HI GCNGC 6 cut(s) 109, 174, 177, 319, 346, 578
FokI GGATG 6 cut(s) 179, 203, 305, 504, 635, 776
Fsp4HI GCNGC 6 cut(s) 109, 174, 177, 319, 346, 578
GluI GCNGC 6 cut(s) 109, 174, 177, 319, 346, 578
HaeIII GGCC 1 cut(s) 173
HapII CCGG 1 cut(s) 542
Hin1II CATG 4 cut(s) 433, 448, 541, 589
HinfI GANTC 3 cut(s) 673, 689, 733
HpaII CCGG 1 cut(s) 542
HphI GGTGA 2 cut(s) 268, 742
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 5 cut(s) 402, 426, 672, 723, 738
Hpy188III TCNNGA 3 cut(s) 290, 483, 649
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 9 cut(s) 35, 52, 170, 376, 480, 496, 604, 733, 739
HpyCH4IV ACGT 2 cut(s) 498, 561
HpyCH4V TGCA 1 cut(s) 577
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 586
HpyF3I CTNAG 3 cut(s) 301, 373, 702
HpySE526I ACGT 2 cut(s) 498, 561
Hsp92II CATG 4 cut(s) 433, 448, 541, 589
Kzo9I GATC 6 cut(s) 86, 286, 388, 479, 567, 684
LmnI GCTCC 2 cut(s) 408, 635
Lsp1109I GCAGC 2 cut(s) 188, 589
MaeII ACGT 2 cut(s) 498, 561
MaeIII GTNAC 2 cut(s) 274, 350
MalI GATC 6 cut(s) 88, 288, 390, 481, 569, 686
MboI GATC 6 cut(s) 86, 286, 388, 479, 567, 684
MboII GAAGA 6 cut(s) 361, 569, 577, 638, 721, 764
MflI RGATCY 1 cut(s) 567
MluCI AATT 1 cut(s) 68
MmeI TCCRAC 3 cut(s) 70, 234, 425
MseI TTAA 2 cut(s) 33, 777
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 2 cut(s) 419, 543
MvaI CCWGG 1 cut(s) 419
MvnI CGCG 1 cut(s) 318
MwoI GCNNNNNNNGC 2 cut(s) 182, 586
NciI CCSGG 1 cut(s) 543
NdeII GATC 6 cut(s) 86, 286, 388, 479, 567, 684
NlaIII CATG 4 cut(s) 433, 448, 541, 589
NlaIV GGNNCC 1 cut(s) 322
NmuCI GTSAC 2 cut(s) 274, 350
NspI RCATGY 2 cut(s) 541, 589
PaeR7I CTCGAG 1 cut(s) 649
PfeI GAWTC 3 cut(s) 673, 689, 733
PflMI CCANNNNNTGG 1 cut(s) 445
PkrI GCNGC 6 cut(s) 110, 175, 178, 320, 347, 579
Psp6I CCWGG 1 cut(s) 417
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 322
PspPI GGNCC 2 cut(s) 171, 436
PstNI CAGNNNCTG 1 cut(s) 424
PsuI RGATCY 1 cut(s) 567
RsaI GTAC 1 cut(s) 680
RsaNI GTAC 1 cut(s) 679
SaqAI TTAA 2 cut(s) 33, 777
SatI GCNGC 6 cut(s) 109, 174, 177, 319, 346, 578
Sau3AI GATC 6 cut(s) 86, 286, 388, 479, 567, 684
Sau96I GGNCC 2 cut(s) 171, 436
ScrFI CCNGG 2 cut(s) 419, 543
SfcI CTRYAG 1 cut(s) 470
Sfr274I CTCGAG 1 cut(s) 649
SinI GGWCC 1 cut(s) 436
SlaI CTCGAG 1 cut(s) 649
SmlI CTYRAG 2 cut(s) 412, 649
SmoI CTYRAG 2 cut(s) 412, 649
Sse9I AATT 1 cut(s) 68
SsiI CCGC 8 cut(s) 109, 174, 183, 318, 324, 343, 346, 633
StyD4I CCNGG 2 cut(s) 417, 541
TaiI ACGT 2 cut(s) 501, 564
TaqI TCGA 5 cut(s) 387, 482, 650, 687, 692
TaqII GACCGA 1 cut(s) 240
TasI AATT 1 cut(s) 68
TatI WGTACW 1 cut(s) 678
TauI GCSGC 4 cut(s) 111, 176, 321, 348
TfiI GAWTC 3 cut(s) 673, 689, 733
Tru1I TTAA 2 cut(s) 33, 777
Tru9I TTAA 2 cut(s) 33, 777
TseFI GTSAC 2 cut(s) 274, 350
TseI GCWGC 2 cut(s) 176, 577
Tsp45I GTSAC 2 cut(s) 274, 350
TspDTI ATGAA 4 cut(s) 309, 433, 639, 722
TspGWI ACGGA 2 cut(s) 209, 381
Van91I CCANNNNNTGG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 436
XapI RAATTY 1 cut(s) 68
XceI RCATGY 2 cut(s) 541, 589
XhoI CTCGAG 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.