Rw1G034250

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004776
Physical Location & Seq
Reverse (-)
62178559 .. 62179268
710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034250.1

Sequence Viewer

Length: 660 bp
ATGGCTTCCTCCCTAGCCCTCCGCCGATTCGTCTTCTCCAATCTCCTCCCCAAACCGCTCTCCTCCTCCGCATTCCGCCCATCCGCGCCCGACGAAACCGACCTGGACTCCTACCGCCGCCGCCACGATTGCTCTCGCTCCCTCCCCGCCGTGACAACTTCATCTCCGGTAATCGTCTACTTAACTTTCTCAATTCCGTTGTTTATTCGTTTAAATTTTCGGGCAATTACGGTTCTGATCGAGTTTGATTTATCGTTACAGATGTGTTTGCTCCTCCCCGTTCTCGGTGGCCGGAACCTGAGCCAGGTGCTGAATCTGGTGGACCAGATCTCGGAGAACCCCTTCCTCTCGGCCACGCGCCGCGGCTGGGACGCGCGTGAGACGGAGGACGGTCTGCATCTCCGGTTCGAAATGCCGGGGCTGGGGAAAGACGACGTGAAGGTTTCGGTGGAGAAGAACACTCTGGTGATCAGAGGCGAAGGCGGACACGTGGACGGTGAAGAAGAGCCGAGGAGGTACTCCACCGGAATTCAGCTGCCGGAGAAGATGTTCATGACGGACGGCGTTAAGGCGGAGATGAAGAACGGCGTGCTCAGGGTGGTTGTGCCGAAGATTAAGGAGGAGGAGAGGAGTGACGTGTTTCACGTGAGGGTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

219

Amino Acids

24.69

Weight (kDa)

6.46

Isoelectric Point (pI)

60.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 125 - 218 3.3e-14 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 58
AccI GTMKAC 1 cut(s) 177
AccII CGCG 5 cut(s) 86, 358, 363, 374, 376
AcoI YGGCCR 2 cut(s) 289, 351
AcsI RAATTY 2 cut(s) 214, 528
AcvI CACGTG 2 cut(s) 490, 646
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 5 cut(s) 284, 304, 331, 367, 422
AflIII ACRYGT 2 cut(s) 487, 636
AjiI CACGTC 2 cut(s) 436, 637
AjnI CCWGG 2 cut(s) 102, 303
AleI CACNNNNGTG 1 cut(s) 464
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
AluBI AGCT 1 cut(s) 535
AluI AGCT 1 cut(s) 535
Alw21I GWGCWC 1 cut(s) 594
Alw26I GTCTC 1 cut(s) 374
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 2 cut(s) 289, 351
ApeKI GCWGC 1 cut(s) 535
ApoI RAATTY 2 cut(s) 214, 528
Asp700I GAANNNNTTC 2 cut(s) 341, 548
AspLEI GCGC 3 cut(s) 88, 360, 376
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 2 cut(s) 478, 509
AsuII TTCGAA 1 cut(s) 408
AvaII GGWCC 1 cut(s) 322
BbrPI CACGTG 2 cut(s) 490, 646
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 594
BbvI GCAGC 1 cut(s) 522
BccI CCATC 1 cut(s) 88
BceAI ACGGC 3 cut(s) 134, 577, 601
BciT130I CCWGG 2 cut(s) 104, 305
BclI TGATCA 1 cut(s) 468
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 1 cut(s) 14
BglII AGATCT 1 cut(s) 327
BisI GCNGC 5 cut(s) 118, 121, 361, 364, 536
BlsI GCNGC 5 cut(s) 119, 122, 362, 365, 537
Bme1390I CCNGG 3 cut(s) 104, 305, 417
Bme18I GGWCC 1 cut(s) 322
BmgBI CACGTC 2 cut(s) 436, 637
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 296
BmrFI CCNGG 3 cut(s) 104, 305, 417
BmsI GCATC 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 25
Bpu10I CCTNAGC 2 cut(s) 299, 593
Bpu14I TTCGAA 1 cut(s) 408
BpuMI CCSGG 1 cut(s) 417
BsaAI YACGTR 2 cut(s) 490, 646
BsaJI CCNNGG 3 cut(s) 361, 416, 509
BsaWI WCCGGW 3 cut(s) 166, 402, 524
BsaXI ACNNNNNCTCC 6 cut(s) 92, 122, 148, 178, 533, 563
Bsc4I CCNNNNNNNGG 5 cut(s) 284, 304, 331, 367, 422
BseBI CCWGG 2 cut(s) 104, 305
BseDI CCNNGG 3 cut(s) 361, 416, 509
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 5 cut(s) 284, 304, 331, 367, 422
BseMII CTCAG 2 cut(s) 290, 607
BseRI GAGGAG 8 cut(s) 35, 52, 55, 263, 526, 635, 638, 643
BseXI GCAGC 1 cut(s) 522
BseYI CCCAGC 2 cut(s) 366, 421
Bsh1236I CGCG 5 cut(s) 86, 358, 363, 374, 376
BshFI GGCC 2 cut(s) 291, 353
BsiHKAI GWGCWC 1 cut(s) 594
BsiSI CCGG 6 cut(s) 167, 292, 403, 416, 525, 539
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 5 cut(s) 284, 304, 331, 367, 422
BsmAI GTCTC 1 cut(s) 374
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 1 cut(s) 383
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 2 cut(s) 291, 353
Bsp119I TTCGAA 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 594
Bsp143I GATC 3 cut(s) 237, 327, 468
BspANI GGCC 2 cut(s) 291, 353
BspCNI CTCAG 2 cut(s) 291, 606
BspFNI CGCG 5 cut(s) 86, 358, 363, 374, 376
BspHI TCATGA 1 cut(s) 552
BspLI GGNNCC 1 cut(s) 296
BspQI GCTCTTC 1 cut(s) 498
BspT104I TTCGAA 1 cut(s) 408
BsrBI CCGCTC 1 cut(s) 58
BssECI CCNNGG 3 cut(s) 361, 416, 509
BssMI GATC 3 cut(s) 237, 327, 468
Bst2UI CCWGG 2 cut(s) 104, 305
Bst4CI ACNGT 3 cut(s) 232, 392, 497
Bst6I CTCTTC 1 cut(s) 498
BstBAI YACGTR 2 cut(s) 490, 646
BstBI TTCGAA 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 590
BstDEI CTNAG 2 cut(s) 299, 593
BstDSI CCRYGG 1 cut(s) 361
BstENI CCTNNNNNAGG 1 cut(s) 302
BstF5I GGATG 1 cut(s) 80
BstFNI CGCG 5 cut(s) 86, 358, 363, 374, 376
BstHHI GCGC 3 cut(s) 88, 360, 376
BstKTI GATC 3 cut(s) 240, 330, 471
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 3 cut(s) 237, 327, 468
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstNI CCWGG 2 cut(s) 104, 305
BstSCI CCNGG 3 cut(s) 102, 303, 415
BstUI CGCG 5 cut(s) 86, 358, 363, 374, 376
BstV1I GCAGC 1 cut(s) 522
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 327
BstYI RGATCY 1 cut(s) 327
BsuRI GGCC 2 cut(s) 291, 353
BtgI CCRYGG 1 cut(s) 361
BtrI CACGTC 2 cut(s) 436, 637
BtsCI GGATG 1 cut(s) 80
Cac8I GCNNGC 1 cut(s) 590
CaiI CAGNNNCTG 1 cut(s) 310
CciI TCATGA 1 cut(s) 552
CfoI GCGC 3 cut(s) 88, 360, 376
Cfr13I GGNCC 1 cut(s) 322
Cfr42I CCGCGG 1 cut(s) 364
CseI GACGC 1 cut(s) 380
Csp6I GTAC 1 cut(s) 517
CviAII CATG 1 cut(s) 553
CviJI RGCY 9 cut(s) 5, 17, 291, 303, 353, 366, 421, 508, 535
CviKI_1 RGCY 9 cut(s) 5, 17, 291, 303, 353, 366, 421, 508, 535
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 2 cut(s) 299, 593
DpnI GATC 3 cut(s) 239, 329, 470
DpnII GATC 3 cut(s) 237, 327, 468
DraI TTTAAA 1 cut(s) 213
EaeI YGGCCR 2 cut(s) 289, 351
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 4 cut(s) 11, 65, 498, 587
Eco47I GGWCC 1 cut(s) 322
Eco72I CACGTG 2 cut(s) 490, 646
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 2 cut(s) 102, 303
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 1 cut(s) 556
FaiI YATR 1 cut(s) 554
FaqI GGGAC 1 cut(s) 383
FatI CATG 1 cut(s) 552
FauI CCCGC 1 cut(s) 154
FbaI TGATCA 1 cut(s) 468
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 5 cut(s) 118, 121, 361, 364, 536
FokI GGATG 1 cut(s) 67
Fsp4HI GCNGC 5 cut(s) 118, 121, 361, 364, 536
FspBI CTAG 1 cut(s) 14
GlaI GCGC 3 cut(s) 87, 359, 375
GluI GCNGC 5 cut(s) 118, 121, 361, 364, 536
GsaI CCCAGC 2 cut(s) 370, 425
HaeIII GGCC 2 cut(s) 291, 353
HapII CCGG 6 cut(s) 167, 292, 403, 416, 525, 539
HgaI GACGC 1 cut(s) 380
HhaI GCGC 3 cut(s) 88, 360, 376
Hin1II CATG 1 cut(s) 556
Hin6I GCGC 3 cut(s) 86, 358, 374
HinP1I GCGC 3 cut(s) 86, 358, 374
HinfI GANTC 3 cut(s) 27, 107, 313
HpaII CCGG 6 cut(s) 167, 292, 403, 416, 525, 539
HphI GGTGA 2 cut(s) 478, 509
Hpy166II GTNNAC 3 cut(s) 178, 322, 493
Hpy188I TCNGA 3 cut(s) 237, 334, 473
Hpy188III TCNNGA 1 cut(s) 553
Hpy8I GTNNAC 3 cut(s) 178, 322, 493
Hpy99I CGWCG 2 cut(s) 95, 437
HpyAV CCTTC 3 cut(s) 352, 433, 473
HpyCH4III ACNGT 3 cut(s) 232, 392, 497
HpyCH4IV ACGT 4 cut(s) 435, 489, 636, 645
HpyCH4V TGCA 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 2 cut(s) 299, 593
HpySE526I ACGT 4 cut(s) 435, 489, 636, 645
Hsp92II CATG 1 cut(s) 556
HspAI GCGC 3 cut(s) 86, 358, 374
Ksp22I TGATCA 1 cut(s) 468
KspI CCGCGG 1 cut(s) 364
Kzo9I GATC 3 cut(s) 237, 327, 468
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 2 cut(s) 143, 276
Lsp1109I GCAGC 1 cut(s) 522
LweI GCATC 1 cut(s) 406
MaeI CTAG 1 cut(s) 14
MaeII ACGT 4 cut(s) 435, 489, 636, 645
MaeIII GTNAC 3 cut(s) 151, 255, 632
MalI GATC 3 cut(s) 239, 329, 470
MbiI CCGCTC 1 cut(s) 58
MboI GATC 3 cut(s) 237, 327, 468
MboII GAAGA 7 cut(s) 25, 466, 512, 515, 556, 592, 622
MflI RGATCY 1 cut(s) 327
MhlI GDGCHC 1 cut(s) 594
MluCI AATT 4 cut(s) 192, 214, 225, 528
MlyI GAGTC 1 cut(s) 101
MroXI GAANNNNTTC 2 cut(s) 341, 548
MseI TTAA 4 cut(s) 182, 212, 567, 615
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 2 cut(s) 363, 535
MspI CCGG 6 cut(s) 167, 292, 403, 416, 525, 539
MspR9I CCNGG 3 cut(s) 104, 305, 417
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 2 cut(s) 104, 305
MvnI CGCG 5 cut(s) 86, 358, 363, 374, 376
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 417
NdeII GATC 3 cut(s) 237, 327, 468
NlaIII CATG 1 cut(s) 556
NlaIV GGNNCC 1 cut(s) 296
NmeAIII GCCGAG 2 cut(s) 329, 534
NmuCI GTSAC 2 cut(s) 151, 632
NspV TTCGAA 1 cut(s) 408
OliI CACNNNNGTG 1 cut(s) 464
PagI TCATGA 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 498
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 2 cut(s) 341, 548
PfeI GAWTC 2 cut(s) 27, 313
PkrI GCNGC 5 cut(s) 119, 122, 362, 365, 537
PleI GAGTC 1 cut(s) 101
PmaCI CACGTG 2 cut(s) 490, 646
PmlI CACGTG 2 cut(s) 490, 646
PpsI GAGTC 1 cut(s) 101
Ppu21I YACGTR 2 cut(s) 490, 646
Psp6I CCWGG 2 cut(s) 102, 303
PspCI CACGTG 2 cut(s) 490, 646
PspFI CCCAGC 2 cut(s) 366, 421
PspGI CCWGG 2 cut(s) 102, 303
PspN4I GGNNCC 1 cut(s) 296
PspPI GGNCC 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 327
PvuII CAGCTG 1 cut(s) 535
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 1 cut(s) 464
SacII CCGCGG 1 cut(s) 364
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 4 cut(s) 182, 212, 567, 615
SatI GCNGC 5 cut(s) 118, 121, 361, 364, 536
Sau3AI GATC 3 cut(s) 237, 327, 468
Sau96I GGNCC 1 cut(s) 322
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 3 cut(s) 104, 305, 417
SduI GDGCHC 1 cut(s) 594
SfaNI GCATC 1 cut(s) 406
Sfr303I CCGCGG 1 cut(s) 364
SfuI TTCGAA 1 cut(s) 408
SgrBI CCGCGG 1 cut(s) 364
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 464
Sse9I AATT 4 cut(s) 192, 214, 225, 528
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 102, 303, 415
TaaI ACNGT 3 cut(s) 232, 392, 497
TaiI ACGT 4 cut(s) 438, 492, 639, 648
TaqI TCGA 2 cut(s) 240, 408
TasI AATT 4 cut(s) 192, 214, 225, 528
TauI GCSGC 4 cut(s) 120, 123, 363, 366
TfiI GAWTC 2 cut(s) 27, 313
Tru1I TTAA 4 cut(s) 182, 212, 567, 615
Tru9I TTAA 4 cut(s) 182, 212, 567, 615
TseFI GTSAC 2 cut(s) 151, 632
TseI GCWGC 1 cut(s) 535
Tsp45I GTSAC 2 cut(s) 151, 632
TspDTI ATGAA 3 cut(s) 150, 541, 593
TspGWI ACGGA 3 cut(s) 186, 398, 572
VpaK11BI GGWCC 1 cut(s) 322
XagI CCTNNNNNAGG 1 cut(s) 302
XapI RAATTY 2 cut(s) 214, 528
XmiI GTMKAC 1 cut(s) 177
XmnI GAANNNNTTC 2 cut(s) 341, 548
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.