Rorug07G0074200

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004776
Physical Location & Seq
Reverse (-)
5721988 .. 5724469
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0074200.1

Sequence Viewer

Length: 465 bp
ATGTACGTGACGGCGGTGCGACCGACGGATCTGAATCGGAACACGGAATGGTTTACGTACCCCGGCGTTTGGACCACCTACATTCTCATCGTCTTCTTTTCATGGCTCATCGTTCTCTGCCTCTTCTCTTGCTCTCCCGGCATGGCCTGGACCATCGTCCACCTCGCTCATTTTCTCGTTACGTACCATTTCTTTCACTGGAAGAAGGGAACACCATTTGCTGATGACCAAGGTATCTACAATGCGCTTACTTGGTGGGAGCAGATAGACAATGGCAACCAGCTCACTCGAAACAGAAAGTTTCTAACTGTCGTGCCTGTTGTGTTGTATTTGATAGCATCTCATACAACAGACTATCAAAATCCAATGCTCTTCTTCAACACACTAGCAGTTTTTGTGCTGGTAGTGGCAAAGTTCCCAAACATGCACAAGGTTCGTATATTTGGAATCAATGGTGATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

154

Amino Acids

18.03

Weight (kDa)

8.6

Isoelectric Point (pI)

20.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ORMDL PF04061 12 - 146 8.9e-52 ORMDL family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 14
AclWI GGATC 1 cut(s) 36
AfaI GTAC 3 cut(s) 5, 59, 185
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 1 cut(s) 379
AjnI CCWGG 1 cut(s) 146
AluBI AGCT 1 cut(s) 283
AluI AGCT 1 cut(s) 283
AlwI GGATC 1 cut(s) 36
AoxI GGCC 1 cut(s) 144
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 2 cut(s) 72, 150
AsuC2I CCSGG 2 cut(s) 63, 138
AvaII GGWCC 2 cut(s) 72, 150
BaeI ACNNNNGTAYC 2 cut(s) 217, 250
BbsI GAAGAC 1 cut(s) 85
BccI CCATC 1 cut(s) 161
BceAI ACGGC 1 cut(s) 27
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 1 cut(s) 148
BcnI CCSGG 2 cut(s) 63, 138
BfaI CTAG 1 cut(s) 386
Bme1390I CCNGG 3 cut(s) 63, 138, 148
Bme18I GGWCC 2 cut(s) 72, 150
BmgT120I GGNCC 2 cut(s) 72, 150
BmrFI CCNGG 3 cut(s) 63, 138, 148
BmsI GCATC 1 cut(s) 347
BoxI GACNNNNGTC 1 cut(s) 155
BpiI GAAGAC 1 cut(s) 85
BpuMI CCSGG 2 cut(s) 63, 138
BsaAI YACGTR 3 cut(s) 7, 57, 183
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 2 cut(s) 61, 229
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 1 cut(s) 203
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 2 cut(s) 61, 229
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 203
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 23
BsiSI CCGG 2 cut(s) 63, 138
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 1 cut(s) 146
Bsp143I GATC 2 cut(s) 28, 457
BspACI CCGC 1 cut(s) 14
BspANI GGCC 1 cut(s) 146
BspPI GGATC 1 cut(s) 36
BspQI GCTCTTC 1 cut(s) 377
BsrI ACTGG 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 61, 229
BssMI GATC 2 cut(s) 28, 457
BssT1I CCWWGG 1 cut(s) 229
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 2 cut(s) 128, 377
BstBAI YACGTR 3 cut(s) 7, 57, 183
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 2 cut(s) 31, 460
BstMBI GATC 2 cut(s) 28, 457
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstNI CCWGG 1 cut(s) 148
BstNSI RCATGY 1 cut(s) 427
BstPAI GACNNNNGTC 1 cut(s) 155
BstSCI CCNGG 3 cut(s) 61, 136, 146
BstSNI TACGTA 2 cut(s) 57, 183
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 1 cut(s) 146
BtsIMutI CAGTG 1 cut(s) 196
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 2 cut(s) 72, 150
Csp6I GTAC 3 cut(s) 4, 58, 184
CviAII CATG 3 cut(s) 102, 142, 424
CviJI RGCY 3 cut(s) 106, 146, 283
CviKI_1 RGCY 3 cut(s) 106, 146, 283
CviQI GTAC 3 cut(s) 4, 58, 184
DpnI GATC 2 cut(s) 30, 459
DpnII GATC 2 cut(s) 28, 457
Eam1104I CTCTTC 2 cut(s) 128, 377
EarI CTCTTC 2 cut(s) 128, 377
Eco105I TACGTA 2 cut(s) 57, 183
Eco130I CCWWGG 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 72, 150
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 3 cut(s) 105, 145, 427
FaiI YATR 5 cut(s) 103, 143, 345, 425, 440
FatI CATG 3 cut(s) 101, 141, 423
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 246
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 63, 138
HhaI GCGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 105, 145, 427
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HinfI GANTC 2 cut(s) 34, 447
HpaII CCGG 2 cut(s) 63, 138
Hpy166II GTNNAC 2 cut(s) 54, 160
Hpy188I TCNGA 3 cut(s) 33, 39, 464
Hpy8I GTNNAC 2 cut(s) 54, 160
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 3 cut(s) 6, 56, 182
HpyCH4V TGCA 1 cut(s) 427
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpySE526I ACGT 3 cut(s) 6, 56, 182
Hsp92II CATG 3 cut(s) 105, 145, 427
HspAI GCGC 1 cut(s) 245
Kzo9I GATC 2 cut(s) 28, 457
LguI GCTCTTC 1 cut(s) 377
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 8 cut(s) 76, 133, 151, 160, 184, 293, 330, 386
LweI GCATC 1 cut(s) 347
MaeI CTAG 1 cut(s) 386
MaeII ACGT 3 cut(s) 6, 56, 182
MaeIII GTNAC 2 cut(s) 7, 178
MalI GATC 2 cut(s) 30, 459
MboI GATC 2 cut(s) 28, 457
MboII GAAGA 5 cut(s) 85, 115, 214, 364, 367
MflI RGATCY 1 cut(s) 28
MnlI CCTC 2 cut(s) 131, 173
MspI CCGG 2 cut(s) 63, 138
MspR9I CCNGG 3 cut(s) 63, 138, 148
MvaI CCWGG 1 cut(s) 148
MwoI GCNNNNNNNGC 1 cut(s) 138
NciI CCSGG 2 cut(s) 63, 138
NdeII GATC 2 cut(s) 28, 457
NlaIII CATG 3 cut(s) 105, 145, 427
NmuCI GTSAC 1 cut(s) 7
NspI RCATGY 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 377
PfeI GAWTC 2 cut(s) 34, 447
Ppu21I YACGTR 3 cut(s) 7, 57, 183
PshAI GACNNNNGTC 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 146
PspGI CCWGG 1 cut(s) 146
PspPI GGNCC 2 cut(s) 72, 150
PsuI RGATCY 1 cut(s) 28
RsaI GTAC 3 cut(s) 5, 59, 185
RsaNI GTAC 3 cut(s) 4, 58, 184
SapI GCTCTTC 1 cut(s) 377
Sau3AI GATC 2 cut(s) 28, 457
Sau96I GGNCC 2 cut(s) 72, 150
ScrFI CCNGG 3 cut(s) 63, 138, 148
SetI ASST 8 cut(s) 9, 59, 80, 165, 185, 235, 285, 435
SfaNI GCATC 1 cut(s) 347
SinI GGWCC 2 cut(s) 72, 150
SnaBI TACGTA 2 cut(s) 57, 183
SsiI CCGC 1 cut(s) 14
SspMI CTAG 1 cut(s) 386
StyD4I CCNGG 3 cut(s) 61, 136, 146
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 3 cut(s) 9, 59, 185
TaqI TCGA 1 cut(s) 289
TaqII GACCGA 1 cut(s) 37
TfiI GAWTC 2 cut(s) 34, 447
TscAI CASTG 1 cut(s) 203
TseFI GTSAC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 1 cut(s) 90
TspGWI ACGGA 2 cut(s) 41, 59
TspRI CASTG 1 cut(s) 203
VpaK11BI GGWCC 2 cut(s) 72, 150
XceI RCATGY 1 cut(s) 427
XspI CTAG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.