Rh1BG357900

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004776
Physical Location & Seq
Forward (+)
48911507 .. 48912578
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG357900.1

Sequence Viewer

Length: 615 bp
ATGGCATCCTCCCTAGGCCTCCGCCGACTCCTCTCCTCCAATCTCCTCCCCAAACCACTCTCCTCCCCCGCATTCCGCCCAATCCCCGCCGCCAAACTCCTCAACACCAACGCCTCCCGCCGCTACGACTCCGATGACACCGACGAAACCGACCTGGACTCCTATCGCCGCCACGATCGCTCCCTCCCTCGCCGCCGTGACAGCTTCATCTCCGATGAGTTTGCTCCTCCTCCGTTCTCGGTGGGCCGGAACCTGAGCCAGGTGCTGAATCTGGTGGACCAGATCTCGGAGAACCCCTTCCTCTCGGCCACGCGCCGTGATTGGGACGCGCGTGAGACGGAGGACGGTCTGCGTCTCCGGTTCGAAATGCCCGGGCTGGGGAAAGACGACGTGAAGGTTTCGGTGGAGAAGAACACTCTGGTGATCAGAGGCGAAGGCGGACACGTGGACGGTGAAGAAGAGCCGAGGAGGTACTCCACCGGAATTCAGCTGCCGGAGAAGATGTTTAAGACGGACGGCGTCAAGGCGGAGATGAAGAACGGCGTGCTCAGGGTGGTTGTGCCGAAGATTAAGGAGGAGGAGAGGAGCGACGTGTTTCACGTGAGGGTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

204

Amino Acids

23.16

Weight (kDa)

6.36

Isoelectric Point (pI)

63.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 109 - 203 3.8e-15 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 313, 329, 331
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 1 cut(s) 483
AcvI CACGTG 2 cut(s) 445, 601
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 5 cut(s) 259, 286, 322, 377, 378
AflIII ACRYGT 2 cut(s) 442, 591
AjiI CACGTC 2 cut(s) 391, 592
AjnI CCWGG 2 cut(s) 153, 258
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 2 cut(s) 204, 490
AluI AGCT 2 cut(s) 204, 490
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 329, 359
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 3 cut(s) 16, 244, 306
ApeKI GCWGC 1 cut(s) 490
ApoI RAATTY 1 cut(s) 483
Asp700I GAANNNNTTC 1 cut(s) 296
AspA2I CCTAGG 1 cut(s) 13
AspLEI GCGC 2 cut(s) 315, 331
AspS9I GGNCC 2 cut(s) 244, 277
AsuC2I CCSGG 2 cut(s) 372, 373
AsuHPI GGTGA 2 cut(s) 433, 464
AsuII TTCGAA 1 cut(s) 363
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 1 cut(s) 277
AvrII CCTAGG 1 cut(s) 13
BbrPI CACGTG 2 cut(s) 445, 601
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 1 cut(s) 477
BceAI ACGGC 4 cut(s) 180, 300, 532, 556
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 2 cut(s) 155, 260
BclI TGATCA 1 cut(s) 423
BcnI CCSGG 2 cut(s) 372, 373
BcoDI GTCTC 2 cut(s) 329, 359
BfaI CTAG 1 cut(s) 14
BglII AGATCT 1 cut(s) 282
BisI GCNGC 5 cut(s) 90, 121, 169, 193, 491
BlnI CCTAGG 1 cut(s) 13
BlsI GCNGC 5 cut(s) 91, 122, 170, 194, 492
Bme1390I CCNGG 4 cut(s) 155, 260, 372, 373
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 371
BmgBI CACGTC 2 cut(s) 391, 592
BmgT120I GGNCC 2 cut(s) 244, 277
BmiI GGNNCC 1 cut(s) 251
BmrFI CCNGG 4 cut(s) 155, 260, 372, 373
BmsI GCATC 1 cut(s) 14
Bpu10I CCTNAGC 2 cut(s) 254, 548
Bpu14I TTCGAA 1 cut(s) 363
BpuMI CCSGG 2 cut(s) 372, 373
BsaAI YACGTR 2 cut(s) 445, 601
BsaHI GRCGYC 1 cut(s) 519
BsaJI CCNNGG 3 cut(s) 13, 371, 464
BsaWI WCCGGW 2 cut(s) 357, 479
BsaXI ACNNNNNCTCC 6 cut(s) 143, 164, 173, 194, 488, 518
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 286, 322, 377, 378
BseBI CCWGG 2 cut(s) 155, 260
BseDI CCNNGG 3 cut(s) 13, 371, 464
BseGI GGATG 1 cut(s) 5
BseLI CCNNNNNNNGG 5 cut(s) 259, 286, 322, 377, 378
BseMII CTCAG 2 cut(s) 245, 562
BseXI GCAGC 1 cut(s) 477
BseYI CCCAGC 1 cut(s) 376
Bsh1236I CGCG 3 cut(s) 313, 329, 331
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 3 cut(s) 18, 246, 308
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 5 cut(s) 247, 358, 372, 480, 494
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 5 cut(s) 259, 286, 322, 377, 378
BsmAI GTCTC 2 cut(s) 329, 359
BsmBI CGTCTC 2 cut(s) 329, 359
BsmFI GGGAC 1 cut(s) 338
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 3 cut(s) 18, 246, 308
BsoBI CYCGRG 1 cut(s) 371
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 1 cut(s) 549
Bsp143I GATC 3 cut(s) 175, 282, 423
BspANI GGCC 3 cut(s) 18, 246, 308
BspCNI CTCAG 2 cut(s) 246, 561
BspFNI CGCG 3 cut(s) 313, 329, 331
BspLI GGNNCC 1 cut(s) 251
BspQI GCTCTTC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 363
BssECI CCNNGG 3 cut(s) 13, 371, 464
BssMI GATC 3 cut(s) 175, 282, 423
BssNI GRCGYC 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 13
Bst2UI CCWGG 2 cut(s) 155, 260
Bst4CI ACNGT 2 cut(s) 347, 452
Bst6I CTCTTC 1 cut(s) 453
BstACI GRCGYC 1 cut(s) 519
BstBAI YACGTR 2 cut(s) 445, 601
BstBI TTCGAA 1 cut(s) 363
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 2 cut(s) 254, 548
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 5
BstFNI CGCG 3 cut(s) 313, 329, 331
BstHHI GCGC 2 cut(s) 315, 331
BstKTI GATC 3 cut(s) 178, 285, 426
BstMAI GTCTC 2 cut(s) 329, 359
BstMBI GATC 3 cut(s) 175, 282, 423
BstMCI CGRYCG 1 cut(s) 178
BstMWI GCNNNNNNNGC 2 cut(s) 177, 201
BstNI CCWGG 2 cut(s) 155, 260
BstSCI CCNGG 4 cut(s) 153, 258, 370, 371
BstUI CGCG 3 cut(s) 313, 329, 331
BstV1I GCAGC 1 cut(s) 477
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BsuRI GGCC 3 cut(s) 18, 246, 308
BtrI CACGTC 2 cut(s) 391, 592
BtsCI GGATG 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 545
CaiI CAGNNNCTG 1 cut(s) 265
CfoI GCGC 2 cut(s) 315, 331
Cfr13I GGNCC 2 cut(s) 244, 277
Cfr9I CCCGGG 1 cut(s) 371
CseI GACGC 3 cut(s) 335, 341, 508
Csp6I GTAC 1 cut(s) 472
CviJI RGCY 8 cut(s) 18, 204, 246, 258, 308, 376, 463, 490
CviKI_1 RGCY 8 cut(s) 18, 204, 246, 258, 308, 376, 463, 490
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 2 cut(s) 254, 548
DpnI GATC 3 cut(s) 177, 284, 425
DpnII GATC 3 cut(s) 175, 282, 423
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
EciI GGCGGA 4 cut(s) 11, 65, 453, 542
Eco130I CCWWGG 1 cut(s) 13
Eco147I AGGCCT 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 277
Eco72I CACGTG 2 cut(s) 445, 601
Eco88I CYCGRG 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 483
EcoRII CCWGG 2 cut(s) 153, 258
EcoT14I CCWWGG 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 13
Esp3I CGTCTC 2 cut(s) 329, 359
FaqI GGGAC 1 cut(s) 338
FauI CCCGC 3 cut(s) 76, 94, 125
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 5 cut(s) 90, 121, 169, 193, 491
Fsp4HI GCNGC 5 cut(s) 90, 121, 169, 193, 491
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 314, 330
GluI GCNGC 5 cut(s) 90, 121, 169, 193, 491
GsaI CCCAGC 1 cut(s) 380
HaeIII GGCC 3 cut(s) 18, 246, 308
HapII CCGG 5 cut(s) 247, 358, 372, 480, 494
HgaI GACGC 3 cut(s) 335, 341, 508
HhaI GCGC 2 cut(s) 315, 331
Hin1I GRCGYC 1 cut(s) 519
Hin6I GCGC 2 cut(s) 313, 329
HinP1I GCGC 2 cut(s) 313, 329
HinfI GANTC 4 cut(s) 27, 128, 158, 268
HpaII CCGG 5 cut(s) 247, 358, 372, 480, 494
HphI GGTGA 2 cut(s) 433, 464
Hpy166II GTNNAC 2 cut(s) 277, 448
Hpy188I TCNGA 4 cut(s) 133, 214, 289, 428
Hpy8I GTNNAC 2 cut(s) 277, 448
Hpy99I CGWCG 3 cut(s) 146, 392, 593
HpyAV CCTTC 3 cut(s) 307, 388, 428
HpyCH4III ACNGT 2 cut(s) 347, 452
HpyCH4IV ACGT 4 cut(s) 390, 444, 591, 600
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 201
HpyF3I CTNAG 2 cut(s) 254, 548
HpySE526I ACGT 4 cut(s) 390, 444, 591, 600
Hsp92I GRCGYC 1 cut(s) 519
HspAI GCGC 2 cut(s) 313, 329
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 3 cut(s) 175, 282, 423
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 3 cut(s) 185, 229, 585
Lsp1109I GCAGC 1 cut(s) 477
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 14
MaeII ACGT 4 cut(s) 390, 444, 591, 600
MaeIII GTNAC 1 cut(s) 197
MalI GATC 3 cut(s) 177, 284, 425
MboI GATC 3 cut(s) 175, 282, 423
MboII GAAGA 6 cut(s) 421, 467, 470, 511, 547, 577
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 549
MluCI AATT 1 cut(s) 483
MlyI GAGTC 3 cut(s) 21, 122, 152
MroXI GAANNNNTTC 1 cut(s) 296
MseI TTAA 2 cut(s) 507, 570
MslI CAYNNNNRTG 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 490
MspI CCGG 5 cut(s) 247, 358, 372, 480, 494
MspR9I CCNGG 4 cut(s) 155, 260, 372, 373
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 2 cut(s) 155, 260
MvnI CGCG 3 cut(s) 313, 329, 331
MwoI GCNNNNNNNGC 2 cut(s) 177, 201
NciI CCSGG 2 cut(s) 372, 373
NdeII GATC 3 cut(s) 175, 282, 423
NlaIV GGNNCC 1 cut(s) 251
NmeAIII GCCGAG 2 cut(s) 284, 489
NmuCI GTSAC 1 cut(s) 197
NspV TTCGAA 1 cut(s) 363
OliI CACNNNNGTG 1 cut(s) 419
PceI AGGCCT 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 1 cut(s) 268
PflFI GACNNNGTC 1 cut(s) 518
PkrI GCNGC 5 cut(s) 91, 122, 170, 194, 492
Ple19I CGATCG 1 cut(s) 178
PleI GAGTC 3 cut(s) 21, 122, 152
PmaCI CACGTG 2 cut(s) 445, 601
PmlI CACGTG 2 cut(s) 445, 601
PpsI GAGTC 3 cut(s) 21, 122, 152
Ppu21I YACGTR 2 cut(s) 445, 601
Psp6I CCWGG 2 cut(s) 153, 258
PspCI CACGTG 2 cut(s) 445, 601
PspFI CCCAGC 1 cut(s) 376
PspGI CCWGG 2 cut(s) 153, 258
PspN4I GGNNCC 1 cut(s) 251
PspPI GGNCC 2 cut(s) 244, 277
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 282
PsyI GACNNNGTC 1 cut(s) 518
PvuI CGATCG 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 490
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
RseI CAYNNNNRTG 1 cut(s) 419
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 2 cut(s) 507, 570
SatI GCNGC 5 cut(s) 90, 121, 169, 193, 491
Sau3AI GATC 3 cut(s) 175, 282, 423
Sau96I GGNCC 2 cut(s) 244, 277
SchI GAGTC 3 cut(s) 21, 122, 152
ScrFI CCNGG 4 cut(s) 155, 260, 372, 373
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 1 cut(s) 14
SfuI TTCGAA 1 cut(s) 363
SinI GGWCC 1 cut(s) 277
SmaI CCCGGG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 419
SrfI GCCCGGGC 1 cut(s) 373
Sse9I AATT 1 cut(s) 483
SseBI AGGCCT 1 cut(s) 18
SspMI CTAG 1 cut(s) 14
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 4 cut(s) 153, 258, 370, 371
StyI CCWWGG 1 cut(s) 13
TaaI ACNGT 2 cut(s) 347, 452
TaiI ACGT 4 cut(s) 393, 447, 594, 603
TaqI TCGA 1 cut(s) 363
TasI AATT 1 cut(s) 483
TauI GCSGC 4 cut(s) 92, 123, 171, 195
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 2 cut(s) 507, 570
Tru9I TTAA 2 cut(s) 507, 570
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 1 cut(s) 490
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 2 cut(s) 196, 548
TspGWI ACGGA 3 cut(s) 222, 353, 527
TspMI CCCGGG 1 cut(s) 371
Tth111I GACNNNGTC 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 277
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 1 cut(s) 483
XmaI CCCGGG 1 cut(s) 371
XmaJI CCTAGG 1 cut(s) 13
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.