RLG00000026740

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004776
Physical Location & Seq
Reverse (-)
5438412 .. 5442453
4042 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026740

Sequence Viewer

Length: 726 bp
ATGTCGATTGATGTGGAAGTACATGACCATAGACACTTTGATGTGTTTGCTCCTCCTCCGTTCTCGGTGGGCCGGAACCTGAGCCAGGTGCTGAATCTGGTGGACATCTCGGAGAACCCCTTCCTCTCGGCCACGCGCCGCAGATGGGACGCGCGTGAGACGGAAGACGGTCTGCATCTCCGGTTCGAAATGCCGGGGCTGGGGAAAGACGACGTGAAGGTTTCCGTGGAGAAGAACACTCTGGTGATCAGAGGCGAAGGCGGACGCGTGGACGGTGAAGAAGAGCCGAGGAGGTACTCCACCGGAATTCAGCTGCCGGAGAAGATGTTCAAGACGGACGGCGTCAAGGCGGAGATGAAGAACGGCGTGCTCAGGGTGGTTGTGCCGAAGATTAAGGAGGAGGAGAGGAGTGACGTGTTTCACGCGTGGGCGACCATTACTGGCCCAAACGAGACGTCGACTAGAGGTCCAAAGAGGGCCAAGAATCCCGCGCCAGATGGCGCCAAGGGACTGCCATCATGCCACGTCATTGAAACTGTACTCCCAAAAGGCCCCTGGTGGGCCCCGAAATTTCAGGGAGGATATACGAAACTAGCTCGGCATAATTCCGTTTCGGATATGCGAATATCCGTTCCTGATCCGCCCGATTTGTACTTACCCGGCGACAGAGTTGAAGGTCTAGTTGCCTCCAGTGGAATGCGGGAATTACGTTTTTGCCCACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

242

Amino Acids

26.92

Weight (kDa)

7.11

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 51 - 136 3.1e-14 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 500
AccI GTMKAC 1 cut(s) 458
AccII CGCG 6 cut(s) 136, 152, 154, 267, 425, 491
AciI CCGC 6 cut(s) 139, 261, 350, 489, 641, 700
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 2 cut(s) 306, 569
AcyI GRCGYC 3 cut(s) 342, 455, 501
AfaI GTAC 4 cut(s) 21, 296, 540, 653
AfiI CCNNNNNNNGG 4 cut(s) 85, 145, 200, 559
AflIII ACRYGT 3 cut(s) 265, 414, 423
AgsI TTSAA 3 cut(s) 331, 533, 674
AhdI GACNNNNNGTC 1 cut(s) 465
AjiI CACGTC 3 cut(s) 214, 415, 526
AjnI CCWGG 2 cut(s) 84, 554
AleI CACNNNNGTG 1 cut(s) 242
AloI GAACNNNNNNTCC 2 cut(s) 311, 343
AluBI AGCT 2 cut(s) 313, 596
AluI AGCT 2 cut(s) 313, 596
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 2 cut(s) 152, 446
AlwI GGATC 1 cut(s) 632
AlwNI CAGNNNCTG 1 cut(s) 91
AoxI GGCC 6 cut(s) 70, 129, 442, 477, 550, 561
ApaI GGGCCC 1 cut(s) 565
ApeKI GCWGC 1 cut(s) 313
ApoI RAATTY 2 cut(s) 306, 569
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
Asp700I GAANNNNTTC 2 cut(s) 119, 326
AspLEI GCGC 4 cut(s) 138, 154, 493, 503
AspS9I GGNCC 7 cut(s) 70, 443, 467, 477, 551, 561, 562
AsuC2I CCSGG 2 cut(s) 195, 660
AsuHPI GGTGA 2 cut(s) 256, 287
AsuII TTCGAA 1 cut(s) 186
AvaII GGWCC 1 cut(s) 467
BaeGI GKGCMC 1 cut(s) 565
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 565
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 300
BccI CCATC 3 cut(s) 138, 491, 523
BceAI ACGGC 2 cut(s) 355, 379
BciT130I CCWGG 2 cut(s) 86, 556
BclI TGATCA 1 cut(s) 246
BcnI CCSGG 2 cut(s) 195, 660
BcoDI GTCTC 2 cut(s) 152, 446
BfaI CTAG 3 cut(s) 462, 593, 680
BfoI RGCGCY 1 cut(s) 504
BisI GCNGC 2 cut(s) 139, 314
BlsI GCNGC 2 cut(s) 140, 315
Bme1390I CCNGG 4 cut(s) 86, 195, 556, 660
Bme18I GGWCC 1 cut(s) 467
BmeRI GACNNNNNGTC 1 cut(s) 465
BmgBI CACGTC 3 cut(s) 214, 415, 526
BmgT120I GGNCC 7 cut(s) 70, 443, 467, 477, 551, 561, 562
BmiI GGNNCC 5 cut(s) 77, 502, 553, 563, 564
BmrFI CCNGG 4 cut(s) 86, 195, 556, 660
BmsI GCATC 1 cut(s) 184
BpiI GAAGAC 1 cut(s) 171
BpmI CTGGAG 1 cut(s) 673
Bpu10I CCTNAGC 2 cut(s) 80, 371
Bpu14I TTCGAA 1 cut(s) 186
BpuMI CCSGG 2 cut(s) 195, 660
BsaHI GRCGYC 3 cut(s) 342, 455, 501
BsaJI CCNNGG 5 cut(s) 194, 225, 287, 504, 554
BsaWI WCCGGW 2 cut(s) 180, 302
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 145, 200, 559
Bse1I ACTGG 2 cut(s) 445, 690
BseBI CCWGG 2 cut(s) 86, 556
BseDI CCNNGG 5 cut(s) 194, 225, 287, 504, 554
BseLI CCNNNNNNNGG 4 cut(s) 85, 145, 200, 559
BseMII CTCAG 2 cut(s) 71, 385
BseNI ACTGG 2 cut(s) 445, 690
BseRI GAGGAG 6 cut(s) 42, 45, 304, 413, 416, 421
BseSI GKGCMC 1 cut(s) 565
BseXI GCAGC 1 cut(s) 300
BseYI CCCAGC 1 cut(s) 199
Bsh1236I CGCG 6 cut(s) 136, 152, 154, 267, 425, 491
BshFI GGCC 6 cut(s) 72, 131, 444, 479, 552, 563
BshNI GGYRCC 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 372
BsiSI CCGG 6 cut(s) 73, 181, 194, 303, 317, 660
BslFI GGGAC 2 cut(s) 161, 522
BslI CCNNNNNNNGG 4 cut(s) 85, 145, 200, 559
BsmAI GTCTC 2 cut(s) 152, 446
BsmBI CGTCTC 2 cut(s) 152, 446
BsmFI GGGAC 2 cut(s) 161, 522
BsmI GAATGC 1 cut(s) 702
BsnI GGCC 6 cut(s) 72, 131, 444, 479, 552, 563
Bsp119I TTCGAA 1 cut(s) 186
Bsp120I GGGCCC 1 cut(s) 561
Bsp1286I GDGCHC 2 cut(s) 372, 565
Bsp143I GATC 2 cut(s) 246, 637
BspACI CCGC 6 cut(s) 139, 261, 350, 489, 641, 700
BspANI GGCC 6 cut(s) 72, 131, 444, 479, 552, 563
BspCNI CTCAG 2 cut(s) 72, 384
BspFNI CGCG 6 cut(s) 136, 152, 154, 267, 425, 491
BspLI GGNNCC 5 cut(s) 77, 502, 553, 563, 564
BspPI GGATC 1 cut(s) 632
BspQI GCTCTTC 1 cut(s) 276
BspT104I TTCGAA 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 500
BsrI ACTGG 2 cut(s) 445, 690
BssECI CCNNGG 5 cut(s) 194, 225, 287, 504, 554
BssMI GATC 2 cut(s) 246, 637
BssNI GRCGYC 3 cut(s) 342, 455, 501
BssT1I CCWWGG 1 cut(s) 504
Bst2UI CCWGG 2 cut(s) 86, 556
Bst4CI ACNGT 3 cut(s) 170, 275, 538
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 3 cut(s) 342, 455, 501
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 80, 371
BstDSI CCRYGG 1 cut(s) 225
BstENI CCTNNNNNAGG 1 cut(s) 83
BstFNI CGCG 6 cut(s) 136, 152, 154, 267, 425, 491
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 4 cut(s) 138, 154, 493, 503
BstKTI GATC 2 cut(s) 249, 640
BstMAI GTCTC 2 cut(s) 152, 446
BstMBI GATC 2 cut(s) 246, 637
BstNI CCWGG 2 cut(s) 86, 556
BstSCI CCNGG 4 cut(s) 84, 193, 554, 658
BstSLI GKGCMC 1 cut(s) 565
BstUI CGCG 6 cut(s) 136, 152, 154, 267, 425, 491
BstV1I GCAGC 1 cut(s) 300
BstV2I GAAGAC 1 cut(s) 171
BsuRI GGCC 6 cut(s) 72, 131, 444, 479, 552, 563
BtgI CCRYGG 1 cut(s) 225
BtrI CACGTC 3 cut(s) 214, 415, 526
BtsIMutI CAGTG 1 cut(s) 697
Cac8I GCNNGC 1 cut(s) 368
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 4 cut(s) 138, 154, 493, 503
Cfr13I GGNCC 7 cut(s) 70, 443, 467, 477, 551, 561, 562
CseI GACGC 3 cut(s) 158, 273, 331
Csp6I GTAC 4 cut(s) 20, 295, 539, 652
CviAII CATG 2 cut(s) 23, 519
CviQI GTAC 4 cut(s) 20, 295, 539, 652
DdeI CTNAG 2 cut(s) 80, 371
DinI GGCGCC 1 cut(s) 502
DpnI GATC 2 cut(s) 248, 639
DpnII GATC 2 cut(s) 246, 637
DriI GACNNNNNGTC 1 cut(s) 465
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 276
Eam1105I GACNNNNNGTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 3 cut(s) 276, 365, 630
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 467
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 2 cut(s) 551, 562
EcoRI GAATTC 1 cut(s) 306
EcoRII CCWGG 2 cut(s) 84, 554
EcoT14I CCWWGG 1 cut(s) 504
EcoT38I GRGCYC 1 cut(s) 565
EgeI GGCGCC 1 cut(s) 502
EheI GGCGCC 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 2 cut(s) 152, 446
FaeI CATG 2 cut(s) 26, 522
FaiI YATR 6 cut(s) 24, 30, 520, 585, 603, 620
FaqI GGGAC 2 cut(s) 161, 522
FatI CATG 2 cut(s) 22, 518
FauI CCCGC 2 cut(s) 496, 693
FbaI TGATCA 1 cut(s) 246
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 2 cut(s) 139, 314
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 2 cut(s) 139, 314
FspBI CTAG 3 cut(s) 462, 593, 680
GlaI GCGC 4 cut(s) 137, 153, 492, 502
GluI GCNGC 2 cut(s) 139, 314
GsaI CCCAGC 1 cut(s) 203
GsuI CTGGAG 1 cut(s) 673
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 6 cut(s) 72, 131, 444, 479, 552, 563
HapII CCGG 6 cut(s) 73, 181, 194, 303, 317, 660
HgaI GACGC 3 cut(s) 158, 273, 331
HhaI GCGC 4 cut(s) 138, 154, 493, 503
Hin1I GRCGYC 3 cut(s) 342, 455, 501
Hin1II CATG 2 cut(s) 26, 522
Hin6I GCGC 4 cut(s) 136, 152, 491, 501
HinP1I GCGC 4 cut(s) 136, 152, 491, 501
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HinfI GANTC 2 cut(s) 94, 484
HpaII CCGG 6 cut(s) 73, 181, 194, 303, 317, 660
HphI GGTGA 2 cut(s) 256, 287
Hpy166II GTNNAC 3 cut(s) 103, 271, 459
Hpy188I TCNGA 3 cut(s) 112, 251, 616
Hpy188III TCNNGA 2 cut(s) 331, 635
Hpy8I GTNNAC 3 cut(s) 103, 271, 459
Hpy99I CGWCG 2 cut(s) 215, 460
HpyAV CCTTC 4 cut(s) 130, 211, 251, 668
HpyCH4III ACNGT 3 cut(s) 170, 275, 538
HpyCH4IV ACGT 5 cut(s) 213, 414, 455, 525, 709
HpyCH4V TGCA 1 cut(s) 175
HpyF3I CTNAG 2 cut(s) 80, 371
HpySE526I ACGT 5 cut(s) 213, 414, 455, 525, 709
Hsp92I GRCGYC 3 cut(s) 342, 455, 501
Hsp92II CATG 2 cut(s) 26, 522
HspAI GCGC 4 cut(s) 136, 152, 491, 501
KasI GGCGCC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 2 cut(s) 246, 637
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 1 cut(s) 55
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 1 cut(s) 184
MaeI CTAG 3 cut(s) 462, 593, 680
MaeII ACGT 5 cut(s) 213, 414, 455, 525, 709
MaeIII GTNAC 1 cut(s) 410
MalI GATC 2 cut(s) 248, 639
MboI GATC 2 cut(s) 246, 637
MboII GAAGA 7 cut(s) 176, 244, 290, 293, 334, 370, 400
MhlI GDGCHC 2 cut(s) 372, 565
MluCI AATT 4 cut(s) 306, 569, 604, 704
MluI ACGCGT 2 cut(s) 265, 423
Mly113I GGCGCC 1 cut(s) 501
MroXI GAANNNNTTC 2 cut(s) 119, 326
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 2 cut(s) 39, 242
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 6 cut(s) 73, 181, 194, 303, 317, 660
MspR9I CCNGG 4 cut(s) 86, 195, 556, 660
Mva1269I GAATGC 1 cut(s) 702
MvaI CCWGG 2 cut(s) 86, 556
MvnI CGCG 6 cut(s) 136, 152, 154, 267, 425, 491
NarI GGCGCC 1 cut(s) 501
NciI CCSGG 2 cut(s) 195, 660
NdeII GATC 2 cut(s) 246, 637
NlaIII CATG 2 cut(s) 26, 522
NlaIV GGNNCC 5 cut(s) 77, 502, 553, 563, 564
NmeAIII GCCGAG 3 cut(s) 107, 312, 577
NmuCI GTSAC 1 cut(s) 410
NspV TTCGAA 1 cut(s) 186
OliI CACNNNNGTG 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 276
PctI GAATGC 1 cut(s) 702
PdmI GAANNNNTTC 2 cut(s) 119, 326
PfeI GAWTC 2 cut(s) 94, 484
PflFI GACNNNGTC 1 cut(s) 341
PkrI GCNGC 2 cut(s) 140, 315
PluTI GGCGCC 1 cut(s) 504
Psp6I CCWGG 2 cut(s) 84, 554
PspFI CCCAGC 1 cut(s) 199
PspGI CCWGG 2 cut(s) 84, 554
PspN4I GGNNCC 5 cut(s) 77, 502, 553, 563, 564
PspOMI GGGCCC 1 cut(s) 561
PspPI GGNCC 7 cut(s) 70, 443, 467, 477, 551, 561, 562
PstNI CAGNNNCTG 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 341
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 4 cut(s) 21, 296, 540, 653
RsaNI GTAC 4 cut(s) 20, 295, 539, 652
RseI CAYNNNNRTG 2 cut(s) 39, 242
SalI GTCGAC 1 cut(s) 457
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 2 cut(s) 139, 314
Sau3AI GATC 2 cut(s) 246, 637
Sau96I GGNCC 7 cut(s) 70, 443, 467, 477, 551, 561, 562
ScrFI CCNGG 4 cut(s) 86, 195, 556, 660
SduI GDGCHC 2 cut(s) 372, 565
SfaNI GCATC 1 cut(s) 184
SfoI GGCGCC 1 cut(s) 502
SfuI TTCGAA 1 cut(s) 186
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 39, 242
Sse9I AATT 4 cut(s) 306, 569, 604, 704
SsiI CCGC 6 cut(s) 139, 261, 350, 489, 641, 700
SspDI GGCGCC 1 cut(s) 500
SspMI CTAG 3 cut(s) 462, 593, 680
StyD4I CCNGG 4 cut(s) 84, 193, 554, 658
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 3 cut(s) 170, 275, 538
TaiI ACGT 5 cut(s) 216, 417, 458, 528, 712
TaqI TCGA 3 cut(s) 5, 186, 458
TasI AATT 4 cut(s) 306, 569, 604, 704
TatI WGTACW 3 cut(s) 19, 538, 651
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 2 cut(s) 94, 484
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 1 cut(s) 697
TseFI GTSAC 1 cut(s) 410
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 1 cut(s) 410
TspDTI ATGAA 1 cut(s) 371
TspGWI ACGGA 6 cut(s) 48, 176, 214, 350, 598, 619
TspRI CASTG 1 cut(s) 697
Tth111I GACNNNGTC 1 cut(s) 341
VpaK11BI GGWCC 1 cut(s) 467
XagI CCTNNNNNAGG 1 cut(s) 83
XapI RAATTY 2 cut(s) 306, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 552
XmiI GTMKAC 1 cut(s) 458
XmnI GAANNNNTTC 2 cut(s) 119, 326
XspI CTAG 3 cut(s) 462, 593, 680
ZraI GACGTC 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.