Rw1G034130

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004776
Physical Location & Seq
Forward (+)
62086906 .. 62087612
707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034130.1

Sequence Viewer

Length: 621 bp
ATGGCTTCCTCCCTAGCCCTCCGCCGATTCGTCTTCTCCAATCTCCTCCCCAAACCGCTCTCCTCCTCCGCATTCCGCCCAATCCGCGCCGCCAAACTCTTCAACACCAACGCCTCCCGCCACTACGACTCCGATGAAAGCGACGAAACCGACCTGGACTCCTACCGCCGCCACGATTGCTCTCTCCCTCCCCGCCGTGACAACTTCATCTCCGGTAATCATGTGTTTGCTCCTCCTCCGTTCTCGGTGGACCGGAACCTGAGCCAGGTGCTGAATCTGGTGGACCAGATCTCGGAGAACCCCTTCCTCTCGGCCACGCGCCGCGGCTGGGACGCGCGTGAGACGGAGGACGGTCTGCATCTCCGGTTCGAAATGCCGGGGCTGGGGAAAGACGACGTGAAGGTTTCGGTGGAGAAGAACACTCTGGTGATCAGAGGCGAAGGCGGACACGTGGACGGTGAAGAAGAGCCGAGGAGGTACTCCACCGGAATTCAGCTGCCGGAGAAGATGTTCATGACGGACGGCGTTAAGGCGGAGATGAAGAACGGCGTGCTCAGGGTGGTTGTGCCGAAGATTAAGGAGGAGGAGAGGAGTGACGTGTTTCACGTGAGGGTGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

206

Amino Acids

23.38

Weight (kDa)

6.11

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 112 - 205 2.9e-14 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 58
AccII CGCG 5 cut(s) 87, 319, 324, 335, 337
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 1 cut(s) 489
AcvI CACGTG 2 cut(s) 451, 607
AfaI GTAC 1 cut(s) 479
AfiI CCNNNNNNNGG 4 cut(s) 265, 292, 328, 383
AflIII ACRYGT 2 cut(s) 448, 597
AgsI TTSAA 1 cut(s) 103
AjiI CACGTC 2 cut(s) 397, 598
AjnI CCWGG 2 cut(s) 153, 264
AleI CACNNNNGTG 1 cut(s) 425
AloI GAACNNNNNNTCC 2 cut(s) 494, 526
AluBI AGCT 1 cut(s) 496
AluI AGCT 1 cut(s) 496
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 271
AoxI GGCC 1 cut(s) 312
ApeKI GCWGC 1 cut(s) 496
ApoI RAATTY 1 cut(s) 489
Asp700I GAANNNNTTC 2 cut(s) 302, 509
AspLEI GCGC 3 cut(s) 89, 321, 337
AspS9I GGNCC 2 cut(s) 250, 283
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 2 cut(s) 439, 470
AsuII TTCGAA 1 cut(s) 369
AvaII GGWCC 2 cut(s) 250, 283
BbrPI CACGTG 2 cut(s) 451, 607
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 1 cut(s) 483
BceAI ACGGC 3 cut(s) 180, 538, 562
BciT130I CCWGG 2 cut(s) 155, 266
BclI TGATCA 1 cut(s) 429
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 1 cut(s) 14
BglII AGATCT 1 cut(s) 288
BisI GCNGC 5 cut(s) 90, 169, 322, 325, 497
BlsI GCNGC 5 cut(s) 91, 170, 323, 326, 498
Bme1390I CCNGG 3 cut(s) 155, 266, 378
Bme18I GGWCC 2 cut(s) 250, 283
BmgBI CACGTC 2 cut(s) 397, 598
BmgT120I GGNCC 2 cut(s) 250, 283
BmiI GGNNCC 1 cut(s) 257
BmrFI CCNGG 3 cut(s) 155, 266, 378
BmsI GCATC 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 25
Bpu10I CCTNAGC 2 cut(s) 260, 554
Bpu14I TTCGAA 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 378
BsaAI YACGTR 2 cut(s) 451, 607
BsaJI CCNNGG 3 cut(s) 322, 377, 470
BsaWI WCCGGW 4 cut(s) 212, 252, 363, 485
BsaXI ACNNNNNCTCC 8 cut(s) 113, 143, 143, 173, 194, 224, 494, 524
Bsc4I CCNNNNNNNGG 4 cut(s) 265, 292, 328, 383
BseBI CCWGG 2 cut(s) 155, 266
BseDI CCNNGG 3 cut(s) 322, 377, 470
BseLI CCNNNNNNNGG 4 cut(s) 265, 292, 328, 383
BseMII CTCAG 2 cut(s) 251, 568
BseRI GAGGAG 9 cut(s) 35, 52, 55, 222, 225, 487, 596, 599, 604
BseXI GCAGC 1 cut(s) 483
BseYI CCCAGC 2 cut(s) 327, 382
Bsh1236I CGCG 5 cut(s) 87, 319, 324, 335, 337
BshFI GGCC 1 cut(s) 314
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 6 cut(s) 213, 253, 364, 377, 486, 500
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 4 cut(s) 265, 292, 328, 383
BsmAI GTCTC 1 cut(s) 335
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 1 cut(s) 314
Bsp119I TTCGAA 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 2 cut(s) 288, 429
BspANI GGCC 1 cut(s) 314
BspCNI CTCAG 2 cut(s) 252, 567
BspFNI CGCG 5 cut(s) 87, 319, 324, 335, 337
BspHI TCATGA 1 cut(s) 513
BspLI GGNNCC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 459
BspT104I TTCGAA 1 cut(s) 369
BsrBI CCGCTC 1 cut(s) 58
BssECI CCNNGG 3 cut(s) 322, 377, 470
BssMI GATC 2 cut(s) 288, 429
Bst2UI CCWGG 2 cut(s) 155, 266
Bst4CI ACNGT 2 cut(s) 353, 458
Bst6I CTCTTC 2 cut(s) 104, 459
BstBAI YACGTR 2 cut(s) 451, 607
BstBI TTCGAA 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 551
BstDEI CTNAG 2 cut(s) 260, 554
BstDSI CCRYGG 1 cut(s) 322
BstENI CCTNNNNNAGG 1 cut(s) 263
BstFNI CGCG 5 cut(s) 87, 319, 324, 335, 337
BstHHI GCGC 3 cut(s) 89, 321, 337
BstKTI GATC 2 cut(s) 291, 432
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 2 cut(s) 288, 429
BstMWI GCNNNNNNNGC 2 cut(s) 84, 177
BstNI CCWGG 2 cut(s) 155, 266
BstSCI CCNGG 3 cut(s) 153, 264, 376
BstUI CGCG 5 cut(s) 87, 319, 324, 335, 337
BstV1I GCAGC 1 cut(s) 483
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BsuRI GGCC 1 cut(s) 314
BtgI CCRYGG 1 cut(s) 322
BtrI CACGTC 2 cut(s) 397, 598
Cac8I GCNNGC 1 cut(s) 551
CaiI CAGNNNCTG 1 cut(s) 271
CciI TCATGA 1 cut(s) 513
CfoI GCGC 3 cut(s) 89, 321, 337
Cfr13I GGNCC 2 cut(s) 250, 283
Cfr42I CCGCGG 1 cut(s) 325
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 478
CviAII CATG 2 cut(s) 221, 514
CviJI RGCY 8 cut(s) 5, 17, 264, 314, 327, 382, 469, 496
CviKI_1 RGCY 8 cut(s) 5, 17, 264, 314, 327, 382, 469, 496
CviQI GTAC 1 cut(s) 478
DdeI CTNAG 2 cut(s) 260, 554
DpnI GATC 2 cut(s) 290, 431
DpnII GATC 2 cut(s) 288, 429
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 2 cut(s) 104, 459
EarI CTCTTC 2 cut(s) 104, 459
EciI GGCGGA 4 cut(s) 11, 65, 459, 548
Eco47I GGWCC 2 cut(s) 250, 283
Eco72I CACGTG 2 cut(s) 451, 607
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 2 cut(s) 153, 264
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 2 cut(s) 224, 517
FaiI YATR 2 cut(s) 222, 515
FaqI GGGAC 1 cut(s) 344
FatI CATG 2 cut(s) 220, 513
FauI CCCGC 2 cut(s) 125, 200
FbaI TGATCA 1 cut(s) 429
Fnu4HI GCNGC 5 cut(s) 90, 169, 322, 325, 497
Fsp4HI GCNGC 5 cut(s) 90, 169, 322, 325, 497
FspBI CTAG 1 cut(s) 14
GlaI GCGC 3 cut(s) 88, 320, 336
GluI GCNGC 5 cut(s) 90, 169, 322, 325, 497
GsaI CCCAGC 2 cut(s) 331, 386
HaeIII GGCC 1 cut(s) 314
HapII CCGG 6 cut(s) 213, 253, 364, 377, 486, 500
HgaI GACGC 1 cut(s) 341
HhaI GCGC 3 cut(s) 89, 321, 337
Hin1II CATG 2 cut(s) 224, 517
Hin6I GCGC 3 cut(s) 87, 319, 335
HinP1I GCGC 3 cut(s) 87, 319, 335
HinfI GANTC 4 cut(s) 27, 128, 158, 274
HpaII CCGG 6 cut(s) 213, 253, 364, 377, 486, 500
HphI GGTGA 2 cut(s) 439, 470
Hpy166II GTNNAC 3 cut(s) 250, 283, 454
Hpy188I TCNGA 3 cut(s) 133, 295, 434
Hpy188III TCNNGA 1 cut(s) 514
Hpy8I GTNNAC 3 cut(s) 250, 283, 454
Hpy99I CGWCG 2 cut(s) 146, 398
HpyAV CCTTC 3 cut(s) 313, 394, 434
HpyCH4III ACNGT 2 cut(s) 353, 458
HpyCH4IV ACGT 4 cut(s) 396, 450, 597, 606
HpyCH4V TGCA 1 cut(s) 358
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 177
HpyF3I CTNAG 2 cut(s) 260, 554
HpySE526I ACGT 4 cut(s) 396, 450, 597, 606
Hsp92II CATG 2 cut(s) 224, 517
HspAI GCGC 3 cut(s) 87, 319, 335
Ksp22I TGATCA 1 cut(s) 429
KspI CCGCGG 1 cut(s) 325
Kzo9I GATC 2 cut(s) 288, 429
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 1 cut(s) 483
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 14
MaeII ACGT 4 cut(s) 396, 450, 597, 606
MaeIII GTNAC 2 cut(s) 197, 593
MalI GATC 2 cut(s) 290, 431
MbiI CCGCTC 1 cut(s) 58
MboI GATC 2 cut(s) 288, 429
MboII GAAGA 8 cut(s) 25, 91, 427, 473, 476, 517, 553, 583
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 555
MluCI AATT 1 cut(s) 489
MlyI GAGTC 2 cut(s) 122, 152
MroXI GAANNNNTTC 2 cut(s) 302, 509
MseI TTAA 2 cut(s) 528, 576
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 324, 496
MspI CCGG 6 cut(s) 213, 253, 364, 377, 486, 500
MspR9I CCNGG 3 cut(s) 155, 266, 378
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 2 cut(s) 155, 266
MvnI CGCG 5 cut(s) 87, 319, 324, 335, 337
MwoI GCNNNNNNNGC 2 cut(s) 84, 177
NciI CCSGG 1 cut(s) 378
NdeII GATC 2 cut(s) 288, 429
NlaIII CATG 2 cut(s) 224, 517
NlaIV GGNNCC 1 cut(s) 257
NmeAIII GCCGAG 2 cut(s) 290, 495
NmuCI GTSAC 2 cut(s) 197, 593
NspV TTCGAA 1 cut(s) 369
OliI CACNNNNGTG 1 cut(s) 425
PagI TCATGA 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 603
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 2 cut(s) 302, 509
PfeI GAWTC 2 cut(s) 27, 274
PkrI GCNGC 5 cut(s) 91, 170, 323, 326, 498
PleI GAGTC 2 cut(s) 122, 152
PmaCI CACGTG 2 cut(s) 451, 607
PmlI CACGTG 2 cut(s) 451, 607
PpsI GAGTC 2 cut(s) 122, 152
Ppu21I YACGTR 2 cut(s) 451, 607
Psp6I CCWGG 2 cut(s) 153, 264
PspCI CACGTG 2 cut(s) 451, 607
PspFI CCCAGC 2 cut(s) 327, 382
PspGI CCWGG 2 cut(s) 153, 264
PspN4I GGNNCC 1 cut(s) 257
PspPI GGNCC 2 cut(s) 250, 283
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 1 cut(s) 479
RsaNI GTAC 1 cut(s) 478
RseI CAYNNNNRTG 1 cut(s) 425
SacII CCGCGG 1 cut(s) 325
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 2 cut(s) 528, 576
SatI GCNGC 5 cut(s) 90, 169, 322, 325, 497
Sau3AI GATC 2 cut(s) 288, 429
Sau96I GGNCC 2 cut(s) 250, 283
SchI GAGTC 2 cut(s) 122, 152
ScrFI CCNGG 3 cut(s) 155, 266, 378
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 1 cut(s) 367
Sfr303I CCGCGG 1 cut(s) 325
SfuI TTCGAA 1 cut(s) 369
SgrBI CCGCGG 1 cut(s) 325
SinI GGWCC 2 cut(s) 250, 283
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 1 cut(s) 489
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 153, 264, 376
TaaI ACNGT 2 cut(s) 353, 458
TaiI ACGT 4 cut(s) 399, 453, 600, 609
TaqI TCGA 1 cut(s) 369
TasI AATT 1 cut(s) 489
TauI GCSGC 4 cut(s) 92, 171, 324, 327
TfiI GAWTC 2 cut(s) 27, 274
Tru1I TTAA 2 cut(s) 528, 576
Tru9I TTAA 2 cut(s) 528, 576
TseFI GTSAC 2 cut(s) 197, 593
TseI GCWGC 1 cut(s) 496
Tsp45I GTSAC 2 cut(s) 197, 593
TspDTI ATGAA 4 cut(s) 150, 196, 502, 554
TspGWI ACGGA 3 cut(s) 228, 359, 533
VpaK11BI GGWCC 2 cut(s) 250, 283
XagI CCTNNNNNAGG 1 cut(s) 263
XapI RAATTY 1 cut(s) 489
XmnI GAANNNNTTC 2 cut(s) 302, 509
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.