FvH4_7g27540

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004776
Physical Location & Seq
Forward (+)
20542220 .. 20543199
980 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27540.t1

Sequence Viewer

Length: 600 bp
ATGGTTTCCTCCCTAGCTCTCCGGCGACTCCTCTCCTCCACTCTCCTCCCCAAATCCTCCTCCGCCTTTCCCTCAATCCCCGCCGCCAGGCTCTTCACCACCAACGCCACCCGCCGCTACGACTCCGACGACAGCGACGAAACCGACGTCGACTCCTACCGCCGCCCCCTCCCGCTCCGCCGCAACAACTTCTTCTCCGACGTGTTTGCTCCGTCGCCGTTCTCGGCGGGCCGGAACCTGAGCCAGGTGCTGAATATGGTGGACCAGATCTCAGAGAATCCCTTCCTCTCCGCCACGCGCCGAGGCTGGGACGCGCGTGAGACGGAGGAGGGTTTGCATCTAAGGTTTGACATGCCGGGGCTGGGGAAAGAGGACGTGAAGGTTTCGGTGGAGAAGGACATTTTGGTGATTAGAGGCGAAGGGGGACACGTGGAAGGAGAAGAGGAGCCGAGGAGGTACTCGACGGGGATTCAGCTGCCGGAGAAGGTGTTTAAGACGGACGGCGTCAAGGCGGAGATGAAGAACGGCGTGCTCAAGGTGGTTGTGCCGAAGATTAAGGAGGAGGAGAGGAGTGATGTGTTTCACGTGAGGGTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

200

Amino Acids

22.41

Weight (kDa)

6.02

Isoelectric Point (pI)

61.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 105 - 198 2.8e-15 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccBSI CCGCTC 1 cut(s) 175
AccI GTMKAC 1 cut(s) 150
AccII CGCG 3 cut(s) 298, 314, 316
AcvI CACGTG 2 cut(s) 430, 586
AcyI GRCGYC 2 cut(s) 147, 504
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 4 cut(s) 87, 244, 307, 362
AflIII ACRYGT 2 cut(s) 201, 427
AjiI CACGTC 2 cut(s) 202, 376
AjnI CCWGG 2 cut(s) 86, 243
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
AluBI AGCT 2 cut(s) 17, 475
AluI AGCT 2 cut(s) 17, 475
Alw21I GWGCWC 1 cut(s) 534
Alw26I GTCTC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 250
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 475
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 2 cut(s) 300, 316
AspS9I GGNCC 2 cut(s) 229, 262
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 2 cut(s) 88, 418
AvaII GGWCC 1 cut(s) 262
BbrPI CACGTG 2 cut(s) 430, 586
Bbv12I GWGCWC 1 cut(s) 534
BbvI GCAGC 1 cut(s) 462
BceAI ACGGC 3 cut(s) 202, 517, 541
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 2 cut(s) 88, 245
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 1 cut(s) 14
BglII AGATCT 1 cut(s) 267
BisI GCNGC 5 cut(s) 84, 115, 163, 181, 476
BlsI GCNGC 5 cut(s) 85, 116, 164, 182, 477
Bme1390I CCNGG 3 cut(s) 88, 245, 357
Bme18I GGWCC 1 cut(s) 262
BmgBI CACGTC 2 cut(s) 202, 376
BmgT120I GGNCC 2 cut(s) 229, 262
BmiI GGNNCC 2 cut(s) 236, 447
BmrFI CCNGG 3 cut(s) 88, 245, 357
BmsI GCATC 1 cut(s) 346
Bpu10I CCTNAGC 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 357
BsaAI YACGTR 2 cut(s) 430, 586
BsaHI GRCGYC 2 cut(s) 147, 504
BsaJI CCNNGG 3 cut(s) 301, 356, 449
BsaXI ACNNNNNCTCC 6 cut(s) 137, 167, 179, 209, 473, 503
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 244, 307, 362
BseBI CCWGG 2 cut(s) 88, 245
BseDI CCNNGG 3 cut(s) 301, 356, 449
BseLI CCNNNNNNNGG 4 cut(s) 87, 244, 307, 362
BseMII CTCAG 2 cut(s) 230, 285
BseXI GCAGC 1 cut(s) 462
BseYI CCCAGC 2 cut(s) 306, 361
Bsh1236I CGCG 3 cut(s) 298, 314, 316
BshFI GGCC 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 534
BsiSI CCGG 4 cut(s) 22, 232, 356, 479
BslFI GGGAC 2 cut(s) 323, 438
BslI CCNNNNNNNGG 4 cut(s) 87, 244, 307, 362
BsmAI GTCTC 1 cut(s) 314
BsmBI CGTCTC 1 cut(s) 314
BsmFI GGGAC 2 cut(s) 323, 438
BsnI GGCC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 534
Bsp143I GATC 1 cut(s) 267
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 2 cut(s) 231, 284
BspFNI CGCG 3 cut(s) 298, 314, 316
BspLI GGNNCC 2 cut(s) 236, 447
BspQI GCTCTTC 1 cut(s) 98
BsrBI CCGCTC 1 cut(s) 175
BssECI CCNNGG 3 cut(s) 301, 356, 449
BssMI GATC 1 cut(s) 267
BssNI GRCGYC 2 cut(s) 147, 504
Bst2UI CCWGG 2 cut(s) 88, 245
Bst6I CTCTTC 2 cut(s) 98, 435
BstACI GRCGYC 2 cut(s) 147, 504
BstBAI YACGTR 2 cut(s) 430, 586
BstC8I GCNNGC 2 cut(s) 229, 530
BstDEI CTNAG 3 cut(s) 239, 271, 341
BstENI CCTNNNNNAGG 1 cut(s) 242
BstFNI CGCG 3 cut(s) 298, 314, 316
BstHHI GCGC 2 cut(s) 300, 316
BstKTI GATC 1 cut(s) 270
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 1 cut(s) 267
BstNI CCWGG 2 cut(s) 88, 245
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 3 cut(s) 86, 243, 355
BstUI CGCG 3 cut(s) 298, 314, 316
BstV1I GCAGC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
BsuRI GGCC 1 cut(s) 231
BtrI CACGTC 2 cut(s) 202, 376
Cac8I GCNNGC 2 cut(s) 229, 530
CaiI CAGNNNCTG 1 cut(s) 250
CfoI GCGC 2 cut(s) 300, 316
Cfr13I GGNCC 2 cut(s) 229, 262
CseI GACGC 2 cut(s) 320, 493
Csp6I GTAC 1 cut(s) 457
CviAII CATG 1 cut(s) 352
CviJI RGCY 8 cut(s) 17, 91, 231, 243, 306, 361, 448, 475
CviKI_1 RGCY 8 cut(s) 17, 91, 231, 243, 306, 361, 448, 475
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 3 cut(s) 239, 271, 341
DpnI GATC 1 cut(s) 269
DpnII GATC 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 98, 435
EarI CTCTTC 2 cut(s) 98, 435
EciI GGCGGA 4 cut(s) 52, 167, 280, 527
Eco47I GGWCC 1 cut(s) 262
Eco72I CACGTG 2 cut(s) 430, 586
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 86, 243
Esp3I CGTCTC 1 cut(s) 314
FaeI CATG 1 cut(s) 355
FaiI YATR 2 cut(s) 257, 353
FaqI GGGAC 2 cut(s) 323, 438
FatI CATG 1 cut(s) 351
FauI CCCGC 4 cut(s) 88, 119, 180, 220
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 5 cut(s) 84, 115, 163, 181, 476
Fsp4HI GCNGC 5 cut(s) 84, 115, 163, 181, 476
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 299, 315
GluI GCNGC 5 cut(s) 84, 115, 163, 181, 476
GsaI CCCAGC 2 cut(s) 310, 365
HaeIII GGCC 1 cut(s) 231
HapII CCGG 4 cut(s) 22, 232, 356, 479
HgaI GACGC 2 cut(s) 320, 493
HhaI GCGC 2 cut(s) 300, 316
Hin1I GRCGYC 2 cut(s) 147, 504
Hin1II CATG 1 cut(s) 355
Hin6I GCGC 2 cut(s) 298, 314
HinP1I GCGC 2 cut(s) 298, 314
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 5 cut(s) 27, 122, 152, 277, 469
HpaII CCGG 4 cut(s) 22, 232, 356, 479
HphI GGTGA 2 cut(s) 88, 418
Hpy166II GTNNAC 2 cut(s) 151, 262
Hpy188I TCNGA 3 cut(s) 127, 199, 274
Hpy8I GTNNAC 2 cut(s) 151, 262
Hpy99I CGWCG 7 cut(s) 131, 140, 149, 152, 203, 217, 466
HpyAV CCTTC 6 cut(s) 292, 373, 388, 413, 428, 478
HpyCH4IV ACGT 5 cut(s) 147, 201, 375, 429, 585
HpyCH4V TGCA 1 cut(s) 337
HpyF3I CTNAG 3 cut(s) 239, 271, 341
HpySE526I ACGT 5 cut(s) 147, 201, 375, 429, 585
Hsp92I GRCGYC 2 cut(s) 147, 504
Hsp92II CATG 1 cut(s) 355
HspAI GCGC 2 cut(s) 298, 314
Kzo9I GATC 1 cut(s) 267
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 180, 214, 445
Lsp1109I GCAGC 1 cut(s) 462
LweI GCATC 1 cut(s) 346
MaeI CTAG 1 cut(s) 14
MaeII ACGT 5 cut(s) 147, 201, 375, 429, 585
MalI GATC 1 cut(s) 269
MbiI CCGCTC 1 cut(s) 175
MboI GATC 1 cut(s) 267
MboII GAAGA 5 cut(s) 85, 184, 452, 532, 562
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 1 cut(s) 534
MlyI GAGTC 3 cut(s) 21, 116, 146
MmeI TCCRAC 2 cut(s) 150, 222
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 2 cut(s) 492, 555
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 475
MspI CCGG 4 cut(s) 22, 232, 356, 479
MspR9I CCNGG 3 cut(s) 88, 245, 357
MvaI CCWGG 2 cut(s) 88, 245
MvnI CGCG 3 cut(s) 298, 314, 316
NciI CCSGG 1 cut(s) 357
NdeII GATC 1 cut(s) 267
NlaIII CATG 1 cut(s) 355
NlaIV GGNNCC 2 cut(s) 236, 447
NmeAIII GCCGAG 3 cut(s) 203, 326, 474
NspI RCATGY 1 cut(s) 355
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 135, 144
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 277, 469
PflFI GACNNNGTC 1 cut(s) 503
PkrI GCNGC 5 cut(s) 85, 116, 164, 182, 477
PleI GAGTC 3 cut(s) 21, 116, 146
PmaCI CACGTG 2 cut(s) 430, 586
PmlI CACGTG 2 cut(s) 430, 586
PpsI GAGTC 3 cut(s) 21, 116, 146
Ppu21I YACGTR 2 cut(s) 430, 586
Psp6I CCWGG 2 cut(s) 86, 243
PspCI CACGTG 2 cut(s) 430, 586
PspFI CCCAGC 2 cut(s) 306, 361
PspGI CCWGG 2 cut(s) 86, 243
PspN4I GGNNCC 2 cut(s) 236, 447
PspPI GGNCC 2 cut(s) 229, 262
PstNI CAGNNNCTG 1 cut(s) 250
PsuI RGATCY 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 1 cut(s) 404
SalI GTCGAC 1 cut(s) 149
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 492, 555
SatI GCNGC 5 cut(s) 84, 115, 163, 181, 476
Sau3AI GATC 1 cut(s) 267
Sau96I GGNCC 2 cut(s) 229, 262
SchI GAGTC 3 cut(s) 21, 116, 146
ScrFI CCNGG 3 cut(s) 88, 245, 357
SduI GDGCHC 1 cut(s) 534
SfaNI GCATC 1 cut(s) 346
SinI GGWCC 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 3 cut(s) 86, 243, 355
TaiI ACGT 5 cut(s) 150, 204, 378, 432, 588
TaqI TCGA 2 cut(s) 150, 461
TauI GCSGC 4 cut(s) 86, 117, 165, 183
TfiI GAWTC 2 cut(s) 277, 469
Tru1I TTAA 2 cut(s) 492, 555
Tru9I TTAA 2 cut(s) 492, 555
TseI GCWGC 1 cut(s) 475
TspDTI ATGAA 1 cut(s) 533
TspGWI ACGGA 3 cut(s) 201, 338, 512
Tth111I GACNNNGTC 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 262
XagI CCTNNNNNAGG 1 cut(s) 242
XceI RCATGY 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 150
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 1 cut(s) 14
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.