FvH4_5g20690

Coiled-coil domain containing protein (DUF2052)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006653
Physical Location & Seq
Forward (+)
12284568 .. 12286923
2356 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g20690.t1

Sequence Viewer

Length: 993 bp
ATGGAGACGAAGGCAGTGAGAAGCGAAGCGATAGAGAGCATATCGGAGCGGCTATCGGCGTCGGAGGACCTCTACTTCCCTCGCGCCCTGCAATCCACCATCATCACTCCCTCTCAGCGCAAGTCCATTCTCCTCGACCTCCTCTCCCGCGACGCCGCCGTCTTCTTAGAACGTTACGGATCAAAATTAACGTCTGATGAGCTACTGGAGTTTGATGCGCTGACAGATGACTATGAAGTCAGCTGGCATGTGAAGCATTTGAGGAGTTTACTGAGCCCGACTTCGGATGAATTGAAGTCCAGGGCAGTTCAAGTGAAGAACCGGAGGCGAGCATACCTGAATAAATTGATATTTGATGGAAATTATTTCTCAGAGGATTCAATGAGGGAGAGAGAGCCCTACTTGCATCATGAGTATGTTGGGAAGTTTCAGGATCAGAGTGGTAGGGGCATGGCAAGACCTGGGGAGCGGTGGTCGGACACTTTGTTGAGGAGGTCAGAGGAAGCGATTTTGGTTGCAAAGATTAGAGGGGAGCAACAGAGATTGGGCGTTGATGAGAGTGAATGGGTTGGCGGTGAGAGAATTCAGCAAGTTGAGGAACAGGAGGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAGGAGGAAGAGGAAGAGGAAGAAGAATCTAACAAGGGTAATGGGGGCACACAATCATCAGAGATGCTGGTGGATCCTCCTAATGTGGCCAGTGGTCCTGAAACCGCAGACCATCCGGATGCGCCCAATGTTGCACAAGCAACCACCGCAGGGCAGAGTGAGCAAGCAACTGTATCCGCAGAAGAGTTGCAAGATCTGATGGACCAGTTCACCTTCATCATGCATGAGAAATTTTTGTTGGGAGAAGATCATGAGTACTTGGATTACTCAAAGATAGACAATGATGAGACATTGGATGATCACTGGCAGAGGGAAGCCAACGATGACGCTGAAGAGAAGTACTTTGATGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2052

Protein Analysis

331

Amino Acids

38.03

Weight (kDa)

4.35

Isoelectric Point (pI)

72.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCD97-like_C PF09747 107 - 330 6.9e-54 Coiled-coil domain containing protein 97-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 158, 236
AccBSI CCGCTC 2 cut(s) 49, 469
AccII CGCG 2 cut(s) 84, 150
AccIII TCCGGA 1 cut(s) 751
AciI CCGC 8 cut(s) 49, 148, 156, 469, 573, 741, 783, 813
AclI AACGTT 1 cut(s) 172
AclWI GGATC 4 cut(s) 187, 441, 704, 717
AcoI YGGCCR 1 cut(s) 723
AcsI RAATTY 2 cut(s) 582, 866
AcuI CTGAAG 1 cut(s) 987
AcyI GRCGYC 2 cut(s) 59, 153
AfaI GTAC 2 cut(s) 893, 977
AfiI CCNNNNNNNGG 2 cut(s) 283, 786
AgsI TTSAA 3 cut(s) 295, 311, 381
AjnI CCWGG 2 cut(s) 299, 460
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AluBI AGCT 2 cut(s) 202, 243
AluI AGCT 2 cut(s) 202, 243
Alw26I GTCTC 1 cut(s) 917
AlwI GGATC 4 cut(s) 187, 441, 704, 717
Aor13HI TCCGGA 1 cut(s) 751
AoxI GGCC 1 cut(s) 723
ApoI RAATTY 2 cut(s) 582, 866
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AspLEI GCGC 4 cut(s) 86, 120, 220, 760
AspS9I GGNCC 3 cut(s) 67, 731, 838
AsuHPI GGTGA 2 cut(s) 587, 838
AvaII GGWCC 3 cut(s) 67, 731, 838
BaeGI GKGCMC 1 cut(s) 686
BalI TGGCCA 1 cut(s) 725
BamHI GGATCC 1 cut(s) 709
BanII GRGCYC 2 cut(s) 278, 399
BbsI GAAGAC 1 cut(s) 154
BccI CCATC 4 cut(s) 107, 350, 756, 829
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 301, 462
BciVI GTATCC 1 cut(s) 820
BclI TGATCA 1 cut(s) 934
BcoDI GTCTC 1 cut(s) 917
BfuI GTATCC 1 cut(s) 820
BglII AGATCT 1 cut(s) 829
BisI GCNGC 2 cut(s) 50, 156
BlsI GCNGC 2 cut(s) 51, 157
BmcAI AGTACT 2 cut(s) 893, 977
Bme1390I CCNGG 2 cut(s) 301, 462
Bme18I GGWCC 3 cut(s) 67, 731, 838
BmgT120I GGNCC 3 cut(s) 67, 731, 838
BmiI GGNNCC 1 cut(s) 711
BmrFI CCNGG 2 cut(s) 301, 462
BmsI GCATC 4 cut(s) 205, 415, 690, 745
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 227
BsaHI GRCGYC 2 cut(s) 59, 153
BsaJI CCNNGG 2 cut(s) 300, 461
BsaWI WCCGGW 2 cut(s) 321, 751
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 458, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 786
Bse1I ACTGG 4 cut(s) 210, 726, 841, 944
BseAI TCCGGA 1 cut(s) 751
BseBI CCWGG 2 cut(s) 301, 462
BseDI CCNNGG 2 cut(s) 300, 461
BseGI GGATG 4 cut(s) 292, 748, 760, 937
BseLI CCNNNNNNNGG 2 cut(s) 283, 786
BseMII CTCAG 3 cut(s) 128, 263, 384
BseNI ACTGG 4 cut(s) 210, 726, 841, 944
BseSI GKGCMC 1 cut(s) 686
Bsh1236I CGCG 2 cut(s) 84, 150
BshFI GGCC 1 cut(s) 725
BsiSI CCGG 2 cut(s) 322, 752
BslI CCNNNNNNNGG 2 cut(s) 283, 786
BsmAI GTCTC 1 cut(s) 917
BsnI GGCC 1 cut(s) 725
Bsp1286I GDGCHC 3 cut(s) 278, 399, 686
Bsp13I TCCGGA 1 cut(s) 751
Bsp143I GATC 6 cut(s) 179, 433, 709, 829, 883, 934
BspACI CCGC 8 cut(s) 49, 148, 156, 469, 573, 741, 783, 813
BspANI GGCC 1 cut(s) 725
BspCNI CTCAG 3 cut(s) 127, 264, 383
BspEI TCCGGA 1 cut(s) 751
BspFNI CGCG 2 cut(s) 84, 150
BspHI TCATGA 2 cut(s) 409, 886
BspLI GGNNCC 1 cut(s) 711
BspPI GGATC 4 cut(s) 187, 441, 704, 717
BsrBI CCGCTC 2 cut(s) 49, 469
BsrI ACTGG 4 cut(s) 210, 726, 841, 944
BssECI CCNNGG 2 cut(s) 300, 461
BssMI GATC 6 cut(s) 179, 433, 709, 829, 883, 934
BssNI GRCGYC 2 cut(s) 59, 153
Bst2UI CCWGG 2 cut(s) 301, 462
Bst4CI ACNGT 1 cut(s) 808
Bst6I CTCTTC 5 cut(s) 618, 639, 645, 813, 963
BstACI GRCGYC 2 cut(s) 59, 153
BstC8I GCNNGC 3 cut(s) 245, 330, 801
BstDEI CTNAG 4 cut(s) 114, 166, 272, 370
BstF5I GGATG 4 cut(s) 292, 748, 760, 937
BstFNI CGCG 2 cut(s) 84, 150
BstHHI GCGC 4 cut(s) 86, 120, 220, 760
BstKTI GATC 6 cut(s) 182, 436, 712, 832, 886, 937
BstMAI GTCTC 1 cut(s) 917
BstMBI GATC 6 cut(s) 179, 433, 709, 829, 883, 934
BstMWI GCNNNNNNNGC 4 cut(s) 253, 403, 782, 796
BstNI CCWGG 2 cut(s) 301, 462
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 2 cut(s) 299, 460
BstSLI GKGCMC 1 cut(s) 686
BstUI CGCG 2 cut(s) 84, 150
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 2 cut(s) 709, 829
BstYI RGATCY 2 cut(s) 709, 829
BsuI GTATCC 1 cut(s) 820
BsuRI GGCC 1 cut(s) 725
BtsCI GGATG 4 cut(s) 292, 748, 760, 937
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 3 cut(s) 21, 733, 937
Cac8I GCNNGC 3 cut(s) 245, 330, 801
CciI TCATGA 2 cut(s) 409, 886
CfoI GCGC 4 cut(s) 86, 120, 220, 760
Cfr13I GGNCC 3 cut(s) 67, 731, 838
CseI GACGC 3 cut(s) 48, 161, 971
Csp6I GTAC 2 cut(s) 892, 976
CviAII CATG 6 cut(s) 248, 410, 451, 856, 860, 887
CviJI RGCY 7 cut(s) 52, 202, 243, 276, 397, 725, 953
CviKI_1 RGCY 7 cut(s) 52, 202, 243, 276, 397, 725, 953
CviQI GTAC 2 cut(s) 892, 976
DdeI CTNAG 4 cut(s) 114, 166, 272, 370
DpnI GATC 6 cut(s) 181, 435, 711, 831, 885, 936
DpnII GATC 6 cut(s) 179, 433, 709, 829, 883, 934
DrdI GACNNNNNNGTC 2 cut(s) 158, 236
DseDI GACNNNNNNGTC 2 cut(s) 158, 236
EaeI YGGCCR 1 cut(s) 723
Eam1104I CTCTTC 5 cut(s) 618, 639, 645, 813, 963
EarI CTCTTC 5 cut(s) 618, 639, 645, 813, 963
Eco24I GRGCYC 2 cut(s) 278, 399
Eco47I GGWCC 3 cut(s) 67, 731, 838
Eco57I CTGAAG 1 cut(s) 987
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 582
EcoRII CCWGG 2 cut(s) 299, 460
EcoT22I ATGCAT 1 cut(s) 861
EcoT38I GRGCYC 2 cut(s) 278, 399
FaeI CATG 6 cut(s) 251, 413, 454, 859, 863, 890
FatI CATG 6 cut(s) 247, 409, 450, 855, 859, 886
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 934
Fnu4HI GCNGC 2 cut(s) 50, 156
FokI GGATG 4 cut(s) 299, 735, 767, 944
FriOI GRGCYC 2 cut(s) 278, 399
Fsp4HI GCNGC 2 cut(s) 50, 156
GlaI GCGC 4 cut(s) 85, 119, 219, 759
GluI GCNGC 2 cut(s) 50, 156
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 1 cut(s) 725
HapII CCGG 2 cut(s) 322, 752
HgaI GACGC 3 cut(s) 48, 161, 971
HhaI GCGC 4 cut(s) 86, 120, 220, 760
Hin1I GRCGYC 2 cut(s) 59, 153
Hin1II CATG 6 cut(s) 251, 413, 454, 859, 863, 890
Hin6I GCGC 4 cut(s) 84, 118, 218, 758
HinP1I GCGC 4 cut(s) 84, 118, 218, 758
HinfI GANTC 2 cut(s) 377, 662
HpaII CCGG 2 cut(s) 322, 752
HphI GGTGA 2 cut(s) 587, 838
Hpy166II GTNNAC 2 cut(s) 269, 846
Hpy188III TCNNGA 5 cut(s) 410, 431, 734, 752, 887
Hpy8I GTNNAC 2 cut(s) 269, 846
Hpy99I CGWCG 2 cut(s) 64, 155
HpyAV CCTTC 2 cut(s) 4, 859
HpyCH4III ACNGT 1 cut(s) 808
HpyCH4IV ACGT 2 cut(s) 172, 191
HpyCH4V TGCA 6 cut(s) 91, 406, 518, 770, 826, 859
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 403, 782, 796
HpyF3I CTNAG 4 cut(s) 114, 166, 272, 370
HpySE526I ACGT 2 cut(s) 172, 191
Hsp92I GRCGYC 2 cut(s) 59, 153
Hsp92II CATG 6 cut(s) 251, 413, 454, 859, 863, 890
HspAI GCGC 4 cut(s) 84, 118, 218, 758
Kpn2I TCCGGA 1 cut(s) 751
Ksp22I TGATCA 1 cut(s) 934
Kzo9I GATC 6 cut(s) 179, 433, 709, 829, 883, 934
LmnI GCTCC 3 cut(s) 46, 466, 532
LweI GCATC 4 cut(s) 205, 415, 690, 745
MaeII ACGT 2 cut(s) 172, 191
MaeIII GTNAC 1 cut(s) 173
MalI GATC 6 cut(s) 181, 435, 711, 831, 885, 936
MbiI CCGCTC 2 cut(s) 49, 469
MboI GATC 6 cut(s) 179, 433, 709, 829, 883, 934
MflI RGATCY 2 cut(s) 709, 829
MhlI GDGCHC 3 cut(s) 278, 399, 686
MlsI TGGCCA 1 cut(s) 725
MluCI AATT 6 cut(s) 185, 290, 344, 361, 582, 866
MluNI TGGCCA 1 cut(s) 725
MmeI TCCRAC 2 cut(s) 42, 456
Mox20I TGGCCA 1 cut(s) 725
Mph1103I ATGCAT 1 cut(s) 861
MroI TCCGGA 1 cut(s) 751
MscI TGGCCA 1 cut(s) 725
MseI TTAA 2 cut(s) 188, 991
MslI CAYNNNNRTG 2 cut(s) 414, 753
Msp20I TGGCCA 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 243
MspI CCGG 2 cut(s) 322, 752
MspR9I CCNGG 2 cut(s) 301, 462
MvaI CCWGG 2 cut(s) 301, 462
MvnI CGCG 2 cut(s) 84, 150
MwoI GCNNNNNNNGC 4 cut(s) 253, 403, 782, 796
NdeII GATC 6 cut(s) 179, 433, 709, 829, 883, 934
NlaIII CATG 6 cut(s) 251, 413, 454, 859, 863, 890
NlaIV GGNNCC 1 cut(s) 711
NsiI ATGCAT 1 cut(s) 861
NspI RCATGY 1 cut(s) 251
PagI TCATGA 2 cut(s) 409, 886
PfeI GAWTC 2 cut(s) 377, 662
PkrI GCNGC 2 cut(s) 51, 157
PpuMI RGGWCCY 1 cut(s) 67
Psp1406I AACGTT 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 299, 460
PspGI CCWGG 2 cut(s) 299, 460
PspN4I GGNNCC 1 cut(s) 711
PspPI GGNCC 3 cut(s) 67, 731, 838
PspPPI RGGWCCY 1 cut(s) 67
PsuI RGATCY 2 cut(s) 709, 829
PvuII CAGCTG 1 cut(s) 243
RsaI GTAC 2 cut(s) 893, 977
RsaNI GTAC 2 cut(s) 892, 976
RseI CAYNNNNRTG 2 cut(s) 414, 753
SaqAI TTAA 2 cut(s) 188, 991
SatI GCNGC 2 cut(s) 50, 156
Sau3AI GATC 6 cut(s) 179, 433, 709, 829, 883, 934
Sau96I GGNCC 3 cut(s) 67, 731, 838
ScaI AGTACT 2 cut(s) 893, 977
ScrFI CCNGG 2 cut(s) 301, 462
SduI GDGCHC 3 cut(s) 278, 399, 686
SfaNI GCATC 4 cut(s) 205, 415, 690, 745
SinI GGWCC 3 cut(s) 67, 731, 838
SmiMI CAYNNNNRTG 2 cut(s) 414, 753
Sse9I AATT 6 cut(s) 185, 290, 344, 361, 582, 866
SsiI CCGC 8 cut(s) 49, 148, 156, 469, 573, 741, 783, 813
StyD4I CCNGG 2 cut(s) 299, 460
TaaI ACNGT 1 cut(s) 808
TaiI ACGT 2 cut(s) 175, 194
TaqI TCGA 1 cut(s) 135
TasI AATT 6 cut(s) 185, 290, 344, 361, 582, 866
TatI WGTACW 2 cut(s) 891, 975
TauI GCSGC 2 cut(s) 52, 158
TfiI GAWTC 2 cut(s) 377, 662
Tru1I TTAA 2 cut(s) 188, 991
Tru9I TTAA 2 cut(s) 188, 991
TscAI CASTG 3 cut(s) 21, 733, 944
TspDTI ATGAA 3 cut(s) 249, 303, 841
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 3 cut(s) 21, 733, 944
VpaK11BI GGWCC 3 cut(s) 67, 731, 838
XapI RAATTY 2 cut(s) 582, 866
XceI RCATGY 1 cut(s) 251
ZrmI AGTACT 2 cut(s) 893, 977
Zsp2I ATGCAT 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.