Rh1BG376000

Coiled-coil domain containing protein (DUF2052)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006653
Physical Location & Seq
Reverse (-)
50630123 .. 50637324
7202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG376000.1

Sequence Viewer

Length: 528 bp
ATGGGCAAATTCGATGAACAAAGTTCTGCAGTTCCATCATACAAACGTTATGGATCAAAATTAACATCTGAGGAGCTCCTGGAGTTTGATGCGCTGACAGATGACTATGAAGTCAGCTGGCATGTGAAGCATCTTAGAAGTTTATTGAGTCCGACATCGGATGAATTGAAATCAAGGGCAGTTCAAGTGAAGAACCGGAGGCGAGCCTACCTGAATAAGTTGATATTTGATGGGCAGTATTTCTCGGAGGATTCAATGAGGGAGGAAGAGCACTACTTGCATCATGAGAGGTCAGAGGAAGCGATTTTGGTAGCAAAGATTAGAGGGGAGCAACAGAGGTTGGGTGTTGCTGAGAGTGATTGGGTTGGCGGTGAGAGAATTCAGGAAGTAGAGGAACAGGAGGAGGAGGAGGAAGAATCTAACAACACTAATGGGGGCACACAATCATCAGAGATGCTGGTGGATCCTCCTTATGTGGCCAGTGGTGCTGAAACTGTAGACCATACGTATGCGCCTAATGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2052

Protein Analysis

175

Amino Acids

20.05

Weight (kDa)

4.62

Isoelectric Point (pI)

71.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCD97-like_C PF09747 65 - 96 1.1e-06 Coiled-coil domain containing protein 97-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 498
AciI CCGC 1 cut(s) 369
AclI AACGTT 1 cut(s) 46
AclWI GGATC 3 cut(s) 61, 458, 471
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 2 cut(s) 8, 378
AgsI TTSAA 3 cut(s) 169, 185, 255
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 2 cut(s) 76, 117
AluI AGCT 2 cut(s) 76, 117
Alw21I GWGCWC 2 cut(s) 78, 273
AlwI GGATC 3 cut(s) 61, 458, 471
AoxI GGCC 1 cut(s) 477
ApoI RAATTY 2 cut(s) 8, 378
AspLEI GCGC 2 cut(s) 94, 514
AsuHPI GGTGA 1 cut(s) 383
BaeGI GKGCMC 1 cut(s) 440
BalI TGGCCA 1 cut(s) 479
BamHI GGATCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 78, 273
BccI CCATC 2 cut(s) 43, 224
BciT130I CCWGG 1 cut(s) 80
BfmI CTRYAG 2 cut(s) 27, 495
Bme1390I CCNGG 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 5 cut(s) 79, 139, 289, 444, 511
BpmI CTGGAG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 507
BsaWI WCCGGW 1 cut(s) 195
Bse1I ACTGG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 80
BseGI GGATG 1 cut(s) 166
BseMII CTCAG 2 cut(s) 60, 342
BseNI ACTGG 1 cut(s) 480
BseRI GAGGAG 4 cut(s) 86, 416, 419, 422
BseSI GKGCMC 1 cut(s) 440
BshFI GGCC 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 78, 273
BsiSI CCGG 1 cut(s) 196
BsnI GGCC 1 cut(s) 479
Bsp1286I GDGCHC 3 cut(s) 78, 273, 440
Bsp143I GATC 2 cut(s) 53, 463
BspACI CCGC 1 cut(s) 369
BspANI GGCC 1 cut(s) 479
BspCNI CTCAG 2 cut(s) 61, 343
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 465
BspMAI CTGCAG 1 cut(s) 31
BspPI GGATC 3 cut(s) 61, 458, 471
BspQI GCTCTTC 1 cut(s) 261
BsrI ACTGG 1 cut(s) 480
BssMI GATC 2 cut(s) 53, 463
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 277
BstBAI YACGTR 1 cut(s) 507
BstC8I GCNNGC 2 cut(s) 119, 204
BstDEI CTNAG 3 cut(s) 69, 134, 351
BstF5I GGATG 1 cut(s) 166
BstHHI GCGC 2 cut(s) 94, 514
BstKTI GATC 2 cut(s) 56, 466
BstMBI GATC 2 cut(s) 53, 463
BstMWI GCNNNNNNNGC 3 cut(s) 127, 277, 485
BstNI CCWGG 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 2 cut(s) 27, 495
BstSLI GKGCMC 1 cut(s) 440
BstSNI TACGTA 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
BsuRI GGCC 1 cut(s) 479
BtsCI GGATG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 119, 204
CciI TCATGA 1 cut(s) 283
CfoI GCGC 2 cut(s) 94, 514
CviAII CATG 2 cut(s) 122, 284
CviJI RGCY 4 cut(s) 76, 117, 206, 479
CviKI_1 RGCY 4 cut(s) 76, 117, 206, 479
DdeI CTNAG 3 cut(s) 69, 134, 351
DpnI GATC 2 cut(s) 55, 465
DpnII GATC 2 cut(s) 53, 463
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 477
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 76
Eco105I TACGTA 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 78
Eco53kI GAGCTC 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 78
FaeI CATG 2 cut(s) 125, 287
FaiI YATR 8 cut(s) 40, 51, 108, 123, 285, 474, 504, 510
FatI CATG 2 cut(s) 121, 283
FblI GTMKAC 1 cut(s) 498
FokI GGATG 1 cut(s) 173
FriOI GRGCYC 1 cut(s) 78
GlaI GCGC 2 cut(s) 93, 513
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 1 cut(s) 479
HapII CCGG 1 cut(s) 196
HhaI GCGC 2 cut(s) 94, 514
Hin1II CATG 2 cut(s) 125, 287
Hin6I GCGC 2 cut(s) 92, 512
HinP1I GCGC 2 cut(s) 92, 512
HinfI GANTC 3 cut(s) 148, 251, 416
HpaII CCGG 1 cut(s) 196
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 6 cut(s) 70, 153, 160, 247, 295, 451
Hpy188III TCNNGA 2 cut(s) 284, 383
Hpy8I GTNNAC 1 cut(s) 499
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4IV ACGT 2 cut(s) 46, 506
HpyCH4V TGCA 2 cut(s) 29, 280
HpyF10VI GCNNNNNNNGC 3 cut(s) 127, 277, 485
HpyF3I CTNAG 3 cut(s) 69, 134, 351
HpySE526I ACGT 2 cut(s) 46, 506
Hsp92II CATG 2 cut(s) 125, 287
HspAI GCGC 2 cut(s) 92, 512
Kzo9I GATC 2 cut(s) 53, 463
LguI GCTCTTC 1 cut(s) 261
LmnI GCTCC 3 cut(s) 73, 81, 328
LpnPI CCDG 9 cut(s) 65, 92, 103, 209, 224, 368, 383, 443, 493
LweI GCATC 5 cut(s) 79, 139, 289, 444, 511
MaeII ACGT 2 cut(s) 46, 506
MalI GATC 2 cut(s) 55, 465
MboI GATC 2 cut(s) 53, 463
MboII GAAGA 3 cut(s) 202, 278, 425
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 3 cut(s) 78, 273, 440
MlsI TGGCCA 1 cut(s) 479
MluCI AATT 4 cut(s) 8, 59, 164, 378
MluNI TGGCCA 1 cut(s) 479
MlyI GAGTC 1 cut(s) 157
MmeI TCCRAC 1 cut(s) 176
Mox20I TGGCCA 1 cut(s) 479
MscI TGGCCA 1 cut(s) 479
MseI TTAA 2 cut(s) 62, 526
MslI CAYNNNNRTG 1 cut(s) 507
Msp20I TGGCCA 1 cut(s) 479
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 1 cut(s) 196
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
MwoI GCNNNNNNNGC 3 cut(s) 127, 277, 485
NdeII GATC 2 cut(s) 53, 463
NlaIII CATG 2 cut(s) 125, 287
NlaIV GGNNCC 1 cut(s) 465
NspI RCATGY 1 cut(s) 125
PagI TCATGA 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 251, 416
PfoI TCCNGGA 1 cut(s) 78
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 507
Psp124BI GAGCTC 1 cut(s) 78
Psp1406I AACGTT 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 465
PstI CTGCAG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 463
PvuII CAGCTG 1 cut(s) 117
RseI CAYNNNNRTG 1 cut(s) 507
SacI GAGCTC 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 261
SaqAI TTAA 2 cut(s) 62, 526
Sau3AI GATC 2 cut(s) 53, 463
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 3 cut(s) 78, 273, 440
SetI ASST 7 cut(s) 49, 78, 119, 213, 293, 341, 509
SfaNI GCATC 5 cut(s) 79, 139, 289, 444, 511
SfcI CTRYAG 2 cut(s) 27, 495
SmiMI CAYNNNNRTG 1 cut(s) 507
SnaBI TACGTA 1 cut(s) 507
Sse9I AATT 4 cut(s) 8, 59, 164, 378
SsiI CCGC 1 cut(s) 369
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 496
TaiI ACGT 2 cut(s) 49, 509
TaqI TCGA 1 cut(s) 12
TasI AATT 4 cut(s) 8, 59, 164, 378
TfiI GAWTC 2 cut(s) 251, 416
Tru1I TTAA 2 cut(s) 62, 526
Tru9I TTAA 2 cut(s) 62, 526
TscAI CASTG 1 cut(s) 487
TspDTI ATGAA 3 cut(s) 30, 123, 177
TspRI CASTG 1 cut(s) 487
XapI RAATTY 2 cut(s) 8, 378
XceI RCATGY 1 cut(s) 125
XmiI GTMKAC 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.