Rh1DG406600

Coiled-coil domain containing protein (DUF2052)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006653
Physical Location & Seq
Reverse (-)
62538095 .. 62541932
3838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG406600.1

Sequence Viewer

Length: 534 bp
ATGGCATGTACTGTCCATGAGAATTACATGAGAAAGAAGGGGGAACTCTGGGAACGTTATGGATCAAAATTAACATCTGAGGAGCTCCTGGAGTTTGATGCGCTGACAGATGACTATGAAGTCAGCTGGCATGTGAAGCATCTTAGAAGTTTATTGAGTCCGACATCGGATGAATTGAAATCAAGGGCAGTTCAAGTGAAGAACCGGAGGCGAGCCTACCTGAATAAGTTGATATTTGATGGGCAGTATTTCTCGGAGGATTCAATGAGGGAGGAAGAGCACTACTTGCATCATGAGTCAGAGGAAGCGATTTTGGTAGCAAAGATTAGAGGGGAGCAACAGAGGTTGGGTGTTGCTGAGAGTGATTGGGTTGGCGGTGAGAGAATTCAGGAAGTAGAGGAACAGGAGGAGGAGGAGGAAGAATCTAACAACACTAATGGGGGCACACAATCATCAGAGATGCTGGTGGATCCTCCTTATGTGGCCAGTGGTGCTGAAACTGTAGACCATACGTATGCGCCTAATGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2052

Protein Analysis

177

Amino Acids

20.44

Weight (kDa)

4.65

Isoelectric Point (pI)

67.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCD97-like_C PF09747 68 - 99 9.9e-07 Coiled-coil domain containing protein 97-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccI GTMKAC 1 cut(s) 504
AciI CCGC 1 cut(s) 375
AclI AACGTT 1 cut(s) 55
AclWI GGATC 3 cut(s) 70, 464, 477
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 1 cut(s) 384
AfaI GTAC 1 cut(s) 10
AgsI TTSAA 3 cut(s) 178, 194, 264
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 2 cut(s) 85, 126
AluI AGCT 2 cut(s) 85, 126
Alw21I GWGCWC 2 cut(s) 87, 282
AlwI GGATC 3 cut(s) 70, 464, 477
AoxI GGCC 1 cut(s) 483
ApoI RAATTY 1 cut(s) 384
AspLEI GCGC 2 cut(s) 103, 520
AsuHPI GGTGA 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 446
BalI TGGCCA 1 cut(s) 485
BamHI GGATCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 87
Bbv12I GWGCWC 2 cut(s) 87, 282
BccI CCATC 1 cut(s) 233
BciT130I CCWGG 1 cut(s) 89
BfmI CTRYAG 1 cut(s) 501
Bme1390I CCNGG 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 5 cut(s) 88, 148, 298, 450, 517
BpmI CTGGAG 1 cut(s) 110
BsaAI YACGTR 1 cut(s) 513
BsaWI WCCGGW 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 89
BseGI GGATG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 69, 348
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 4 cut(s) 95, 422, 425, 428
BseSI GKGCMC 1 cut(s) 446
BshFI GGCC 1 cut(s) 485
BsiHKAI GWGCWC 2 cut(s) 87, 282
BsiSI CCGG 1 cut(s) 205
BsnI GGCC 1 cut(s) 485
Bsp1286I GDGCHC 3 cut(s) 87, 282, 446
Bsp143I GATC 2 cut(s) 62, 469
BspACI CCGC 1 cut(s) 375
BspANI GGCC 1 cut(s) 485
BspCNI CTCAG 2 cut(s) 70, 349
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 471
BspPI GGATC 3 cut(s) 70, 464, 477
BspQI GCTCTTC 1 cut(s) 270
BsrI ACTGG 1 cut(s) 486
BssMI GATC 2 cut(s) 62, 469
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 2 cut(s) 13, 502
Bst6I CTCTTC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 286
BstBAI YACGTR 1 cut(s) 513
BstC8I GCNNGC 2 cut(s) 128, 213
BstDEI CTNAG 3 cut(s) 78, 143, 357
BstF5I GGATG 1 cut(s) 175
BstHHI GCGC 2 cut(s) 103, 520
BstKTI GATC 2 cut(s) 65, 472
BstMBI GATC 2 cut(s) 62, 469
BstMWI GCNNNNNNNGC 3 cut(s) 136, 286, 491
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 2 cut(s) 9, 134
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 446
BstSNI TACGTA 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BsuRI GGCC 1 cut(s) 485
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 493
Cac8I GCNNGC 2 cut(s) 128, 213
CciI TCATGA 1 cut(s) 292
CfoI GCGC 2 cut(s) 103, 520
Csp6I GTAC 1 cut(s) 9
CviAII CATG 5 cut(s) 6, 17, 28, 131, 293
CviJI RGCY 4 cut(s) 85, 126, 215, 485
CviKI_1 RGCY 4 cut(s) 85, 126, 215, 485
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 3 cut(s) 78, 143, 357
DpnI GATC 2 cut(s) 64, 471
DpnII GATC 2 cut(s) 62, 469
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Ecl136II GAGCTC 1 cut(s) 85
Eco105I TACGTA 1 cut(s) 513
Eco24I GRGCYC 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 85
EcoRI GAATTC 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 87
FaeI CATG 5 cut(s) 9, 20, 31, 134, 296
FatI CATG 5 cut(s) 5, 16, 27, 130, 292
FblI GTMKAC 1 cut(s) 504
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 1 cut(s) 87
GlaI GCGC 2 cut(s) 102, 519
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 1 cut(s) 485
HapII CCGG 1 cut(s) 205
HhaI GCGC 2 cut(s) 103, 520
Hin1II CATG 5 cut(s) 9, 20, 31, 134, 296
Hin6I GCGC 2 cut(s) 101, 518
HinP1I GCGC 2 cut(s) 101, 518
HinfI GANTC 4 cut(s) 157, 260, 296, 422
HpaII CCGG 1 cut(s) 205
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 6 cut(s) 79, 162, 169, 256, 301, 457
Hpy188III TCNNGA 2 cut(s) 293, 389
Hpy8I GTNNAC 1 cut(s) 505
HpyAV CCTTC 1 cut(s) 31
HpyCH4III ACNGT 2 cut(s) 13, 502
HpyCH4IV ACGT 2 cut(s) 55, 512
HpyCH4V TGCA 1 cut(s) 289
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 286, 491
HpyF3I CTNAG 3 cut(s) 78, 143, 357
HpySE526I ACGT 2 cut(s) 55, 512
Hsp92II CATG 5 cut(s) 9, 20, 31, 134, 296
HspAI GCGC 2 cut(s) 101, 518
Kzo9I GATC 2 cut(s) 62, 469
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 3 cut(s) 82, 90, 334
LweI GCATC 5 cut(s) 88, 148, 298, 450, 517
MaeII ACGT 2 cut(s) 55, 512
MalI GATC 2 cut(s) 64, 471
MboI GATC 2 cut(s) 62, 469
MboII GAAGA 3 cut(s) 211, 287, 431
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 3 cut(s) 87, 282, 446
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 4 cut(s) 22, 68, 173, 384
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 2 cut(s) 166, 305
MmeI TCCRAC 1 cut(s) 185
Mox20I TGGCCA 1 cut(s) 485
MscI TGGCCA 1 cut(s) 485
MseI TTAA 2 cut(s) 71, 532
MslI CAYNNNNRTG 1 cut(s) 513
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 1 cut(s) 205
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 3 cut(s) 136, 286, 491
NdeII GATC 2 cut(s) 62, 469
NlaIII CATG 5 cut(s) 9, 20, 31, 134, 296
NlaIV GGNNCC 1 cut(s) 471
NspI RCATGY 2 cut(s) 9, 134
PagI TCATGA 1 cut(s) 292
PciSI GCTCTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 260, 422
PfoI TCCNGGA 1 cut(s) 87
PleI GAGTC 2 cut(s) 165, 304
PpsI GAGTC 2 cut(s) 165, 304
Ppu21I YACGTR 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 87
Psp1406I AACGTT 1 cut(s) 55
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 471
PsuI RGATCY 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 126
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 1 cut(s) 513
SacI GAGCTC 1 cut(s) 87
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 2 cut(s) 71, 532
Sau3AI GATC 2 cut(s) 62, 469
SchI GAGTC 2 cut(s) 166, 305
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 3 cut(s) 87, 282, 446
SetI ASST 6 cut(s) 58, 87, 128, 222, 347, 515
SfaNI GCATC 5 cut(s) 88, 148, 298, 450, 517
SfcI CTRYAG 1 cut(s) 501
SmiMI CAYNNNNRTG 1 cut(s) 513
SnaBI TACGTA 1 cut(s) 513
Sse9I AATT 4 cut(s) 22, 68, 173, 384
SsiI CCGC 1 cut(s) 375
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 13, 502
TaiI ACGT 2 cut(s) 58, 515
TasI AATT 4 cut(s) 22, 68, 173, 384
TatI WGTACW 1 cut(s) 8
TfiI GAWTC 2 cut(s) 260, 422
Tru1I TTAA 2 cut(s) 71, 532
Tru9I TTAA 2 cut(s) 71, 532
TscAI CASTG 1 cut(s) 493
TspDTI ATGAA 2 cut(s) 132, 186
TspRI CASTG 1 cut(s) 493
XapI RAATTY 1 cut(s) 384
XceI RCATGY 2 cut(s) 9, 134
XmiI GTMKAC 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.