Rroxscaffold_3G00251530

Coiled-coil domain containing protein (DUF2052)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006653
Physical Location & Seq
Reverse (-)
45381273 .. 45383698
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00251530.1

Sequence Viewer

Length: 1080 bp
ATGGAGACGAAGGTAGTGAGAAGCGAAGCGATAGAGAGCATATCGGACAGGCTATCGGCGTCGGAGGACCTCTACTTCCCTCGCGCCCTGCAATCCACCACCATCACCCCCTCTCAGCGCAAGGCCATTCTCCTCGACCTCCTCTCCCGCGACGCCGCCGTTTTCTTAGAACGTTATGGATCGAAATTAACATCTGAGGAGCTCCTGGAGTTTGATGCGCTGACAGATGACTATGAAGTCAGCTGGCATGTGAAGCATCTTAGAAGTTTATTGAGTCCGACATCGGATGAATTGAAATCAAGGGCAGTTCAAGTGAAGAACAGGAGGCGAGCCTACCTGAATAAGTTGATATTTGATGGGCAGTATTTCTCAGAGGATTCAATGAGGGAGCGAGAGCCCTACTTGCATCATGAGTATGTGGGGAAGTTTCAGGATCCGAGTGGTAGGAGCATGGCAAGACCCGGGGAGCGGTGGTCAGATACTTTGTTGAGGAGGTCAGAGGAAGCGATTTTGGTAGCAAAGATTAGAGGGGAGCAACAGAGGTTGGGTGTTGCCGAGAGTGATTGGGTTGGCGGTGAGAGAATCCAGGAAGTAGAGGAACAGGAGGAGGAGGAAGAAGAGGAGGAAGAGGAAGAGGAAGAAGAAGAGGAGGAGGAGGAGGAGGAAGAATCTAACGAGGCTAATGGGGGCACACAATCATCAGAGATGCTGGTGGATCCTCCTAATGTGGCCAGTGGTGCTGAAACTGCAGACCATCCGGATGCGCCCAATGTTGCTCAAGCAACCACAGCAGGGCAGAGTGAGCAAGCAACTGTATCTGCAGAAGAGTTGCAAGATCTGATGGACCAGTTCACCTACATCATGCATGAGAAATTTTTGTTAGGAGAAGATCACGAGTACTTAGATTACTCAAAGATAGACAACGACGAGACATTGGATGATCACTGGCAGAGAGAAGCCAACCATGACGCTGAAGAGAAGTACTTTGATGACATTATTGATGAGGATGAGACATTGAATGATCACCGGCAGAGAGAAGTGAACCATGACGCTGAAGAGAAGTACTTTGATGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2052

Protein Analysis

359

Amino Acids

41.6

Weight (kDa)

4.31

Isoelectric Point (pI)

72.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCD97-like_C PF09747 107 - 331 1.5e-53 Coiled-coil domain containing protein 97-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AccBSI CCGCTC 1 cut(s) 469
AccII CGCG 2 cut(s) 84, 150
AccIII TCCGGA 1 cut(s) 757
AciI CCGC 4 cut(s) 148, 156, 469, 573
AclI AACGTT 1 cut(s) 172
AclWI GGATC 5 cut(s) 187, 428, 441, 710, 723
AcoI YGGCCR 1 cut(s) 729
AcsI RAATTY 1 cut(s) 872
AcuI CTGAAG 2 cut(s) 993, 1074
AcyI GRCGYC 2 cut(s) 59, 153
AfaI GTAC 3 cut(s) 899, 983, 1064
AfiI CCNNNNNNNGG 2 cut(s) 468, 792
AgsI TTSAA 4 cut(s) 295, 311, 381, 1018
AjnI CCWGG 2 cut(s) 204, 585
AluBI AGCT 2 cut(s) 202, 243
AluI AGCT 2 cut(s) 202, 243
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 923, 1004
AlwI GGATC 5 cut(s) 187, 428, 441, 710, 723
Ama87I CYCGRG 1 cut(s) 461
Aor13HI TCCGGA 1 cut(s) 757
AoxI GGCC 2 cut(s) 123, 729
ApoI RAATTY 1 cut(s) 872
AspLEI GCGC 4 cut(s) 86, 120, 220, 766
AspS9I GGNCC 2 cut(s) 67, 844
AsuC2I CCSGG 2 cut(s) 462, 463
AsuHPI GGTGA 4 cut(s) 97, 587, 844, 1016
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 2 cut(s) 67, 844
BaeGI GKGCMC 1 cut(s) 692
BalI TGGCCA 1 cut(s) 731
BamHI GGATCC 2 cut(s) 433, 715
BanII GRGCYC 2 cut(s) 204, 399
BauI CACGAG 1 cut(s) 893
Bbv12I GWGCWC 1 cut(s) 204
BccI CCATC 4 cut(s) 110, 350, 762, 835
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 206, 587
BclI TGATCA 2 cut(s) 940, 1021
BcnI CCSGG 2 cut(s) 462, 463
BcoDI GTCTC 2 cut(s) 923, 1004
BfmI CTRYAG 2 cut(s) 747, 819
BglII AGATCT 1 cut(s) 835
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
BmcAI AGTACT 3 cut(s) 899, 983, 1064
Bme1390I CCNGG 4 cut(s) 206, 462, 463, 587
Bme18I GGWCC 2 cut(s) 67, 844
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 67, 844
BmiI GGNNCC 2 cut(s) 435, 717
BmrFI CCNGG 4 cut(s) 206, 462, 463, 587
BmsI GCATC 5 cut(s) 205, 265, 415, 696, 751
BpmI CTGGAG 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 762
BpuMI CCSGG 2 cut(s) 462, 463
BsaHI GRCGYC 2 cut(s) 59, 153
BsaJI CCNNGG 2 cut(s) 461, 462
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 458, 488
Bsc4I CCNNNNNNNGG 2 cut(s) 468, 792
Bse118I RCCGGY 1 cut(s) 1026
Bse1I ACTGG 3 cut(s) 732, 847, 950
BseAI TCCGGA 1 cut(s) 757
BseBI CCWGG 2 cut(s) 206, 587
BseDI CCNNGG 2 cut(s) 461, 462
BseGI GGATG 5 cut(s) 292, 754, 766, 943, 1012
BseLI CCNNNNNNNGG 2 cut(s) 468, 792
BseMII CTCAG 3 cut(s) 128, 186, 384
BseNI ACTGG 3 cut(s) 732, 847, 950
BseSI GKGCMC 1 cut(s) 692
Bsh1236I CGCG 2 cut(s) 84, 150
BshFI GGCC 2 cut(s) 125, 731
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 462, 758, 1027
BslI CCNNNNNNNGG 2 cut(s) 468, 792
BsmAI GTCTC 2 cut(s) 923, 1004
BsnI GGCC 2 cut(s) 125, 731
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 3 cut(s) 204, 399, 692
Bsp13I TCCGGA 1 cut(s) 757
Bsp143I GATC 7 cut(s) 179, 433, 715, 835, 889, 940, 1021
BspACI CCGC 4 cut(s) 148, 156, 469, 573
BspANI GGCC 2 cut(s) 125, 731
BspCNI CTCAG 3 cut(s) 127, 187, 383
BspEI TCCGGA 1 cut(s) 757
BspFNI CGCG 2 cut(s) 84, 150
BspHI TCATGA 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 435, 717
BspMAI CTGCAG 2 cut(s) 751, 823
BspPI GGATC 5 cut(s) 187, 428, 441, 710, 723
BsrBI CCGCTC 1 cut(s) 469
BsrFI RCCGGY 1 cut(s) 1026
BsrI ACTGG 3 cut(s) 732, 847, 950
BssAI RCCGGY 1 cut(s) 1026
BssECI CCNNGG 2 cut(s) 461, 462
BssMI GATC 7 cut(s) 179, 433, 715, 835, 889, 940, 1021
BssNI GRCGYC 2 cut(s) 59, 153
BssSI CACGAG 1 cut(s) 893
Bst2BI CACGAG 1 cut(s) 893
Bst2UI CCWGG 2 cut(s) 206, 587
Bst4CI ACNGT 1 cut(s) 814
Bst6I CTCTTC 7 cut(s) 612, 621, 627, 639, 819, 969, 1050
BstACI GRCGYC 2 cut(s) 59, 153
BstC8I GCNNGC 3 cut(s) 245, 330, 807
BstDEI CTNAG 6 cut(s) 114, 166, 195, 260, 370, 901
BstF5I GGATG 5 cut(s) 292, 754, 766, 943, 1012
BstFNI CGCG 2 cut(s) 84, 150
BstHHI GCGC 4 cut(s) 86, 120, 220, 766
BstKTI GATC 7 cut(s) 182, 436, 718, 838, 892, 943, 1024
BstMAI GTCTC 2 cut(s) 923, 1004
BstMBI GATC 7 cut(s) 179, 433, 715, 835, 889, 940, 1021
BstMWI GCNNNNNNNGC 6 cut(s) 253, 403, 737, 746, 788, 802
BstNI CCWGG 2 cut(s) 206, 587
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 4 cut(s) 204, 460, 461, 585
BstSFI CTRYAG 2 cut(s) 747, 819
BstSLI GKGCMC 1 cut(s) 692
BstUI CGCG 2 cut(s) 84, 150
BstX2I RGATCY 3 cut(s) 433, 715, 835
BstYI RGATCY 3 cut(s) 433, 715, 835
BsuRI GGCC 2 cut(s) 125, 731
BtsCI GGATG 5 cut(s) 292, 754, 766, 943, 1012
BtsIMutI CAGTG 2 cut(s) 739, 943
Cac8I GCNNGC 3 cut(s) 245, 330, 807
CciI TCATGA 1 cut(s) 409
CfoI GCGC 4 cut(s) 86, 120, 220, 766
Cfr10I RCCGGY 1 cut(s) 1026
Cfr13I GGNCC 2 cut(s) 67, 844
Cfr9I CCCGGG 1 cut(s) 461
CseI GACGC 4 cut(s) 48, 161, 977, 1058
Csp6I GTAC 3 cut(s) 898, 982, 1063
CviAII CATG 7 cut(s) 248, 410, 451, 862, 866, 965, 1046
CviJI RGCY 9 cut(s) 52, 125, 202, 243, 332, 397, 680, 731, 959
CviKI_1 RGCY 9 cut(s) 52, 125, 202, 243, 332, 397, 680, 731, 959
CviQI GTAC 3 cut(s) 898, 982, 1063
DdeI CTNAG 6 cut(s) 114, 166, 195, 260, 370, 901
DpnI GATC 7 cut(s) 181, 435, 717, 837, 891, 942, 1023
DpnII GATC 7 cut(s) 179, 433, 715, 835, 889, 940, 1021
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 1 cut(s) 729
Eam1104I CTCTTC 7 cut(s) 612, 621, 627, 639, 819, 969, 1050
EarI CTCTTC 7 cut(s) 612, 621, 627, 639, 819, 969, 1050
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 2 cut(s) 204, 399
Eco47I GGWCC 2 cut(s) 67, 844
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 2 cut(s) 993, 1074
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 202
EcoO109I RGGNCCY 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 204, 585
EcoT22I ATGCAT 1 cut(s) 867
EcoT38I GRGCYC 2 cut(s) 204, 399
FaeI CATG 7 cut(s) 251, 413, 454, 865, 869, 968, 1049
FatI CATG 7 cut(s) 247, 409, 450, 861, 865, 964, 1045
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 2 cut(s) 940, 1021
Fnu4HI GCNGC 1 cut(s) 156
FokI GGATG 5 cut(s) 299, 741, 773, 950, 1019
FriOI GRGCYC 2 cut(s) 204, 399
Fsp4HI GCNGC 1 cut(s) 156
GlaI GCGC 4 cut(s) 85, 119, 219, 765
GluI GCNGC 1 cut(s) 156
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 2 cut(s) 125, 731
HapII CCGG 3 cut(s) 462, 758, 1027
HgaI GACGC 4 cut(s) 48, 161, 977, 1058
HhaI GCGC 4 cut(s) 86, 120, 220, 766
Hin1I GRCGYC 2 cut(s) 59, 153
Hin1II CATG 7 cut(s) 251, 413, 454, 865, 869, 968, 1049
Hin6I GCGC 4 cut(s) 84, 118, 218, 764
HinP1I GCGC 4 cut(s) 84, 118, 218, 764
HinfI GANTC 4 cut(s) 274, 377, 582, 668
HpaII CCGG 3 cut(s) 462, 758, 1027
HphI GGTGA 4 cut(s) 97, 587, 844, 1016
Hpy166II GTNNAC 2 cut(s) 852, 1042
Hpy188III TCNNGA 4 cut(s) 410, 431, 758, 893
Hpy8I GTNNAC 2 cut(s) 852, 1042
Hpy99I CGWCG 3 cut(s) 64, 155, 929
HpyAV CCTTC 1 cut(s) 4
HpyCH4III ACNGT 1 cut(s) 814
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 6 cut(s) 91, 406, 749, 821, 832, 865
HpyF10VI GCNNNNNNNGC 6 cut(s) 253, 403, 737, 746, 788, 802
HpyF3I CTNAG 6 cut(s) 114, 166, 195, 260, 370, 901
HpySE526I ACGT 1 cut(s) 172
Hsp92I GRCGYC 2 cut(s) 59, 153
Hsp92II CATG 7 cut(s) 251, 413, 454, 865, 869, 968, 1049
HspAI GCGC 4 cut(s) 84, 118, 218, 764
Kpn2I TCCGGA 1 cut(s) 757
Ksp22I TGATCA 2 cut(s) 940, 1021
Kzo9I GATC 7 cut(s) 179, 433, 715, 835, 889, 940, 1021
LmnI GCTCC 6 cut(s) 199, 207, 388, 447, 466, 532
LweI GCATC 5 cut(s) 205, 265, 415, 696, 751
MaeII ACGT 1 cut(s) 172
MalI GATC 7 cut(s) 181, 435, 717, 837, 891, 942, 1023
MbiI CCGCTC 1 cut(s) 469
MboI GATC 7 cut(s) 179, 433, 715, 835, 889, 940, 1021
MflI RGATCY 3 cut(s) 433, 715, 835
MhlI GDGCHC 3 cut(s) 204, 399, 692
MlsI TGGCCA 1 cut(s) 731
MluCI AATT 3 cut(s) 185, 290, 872
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 2 cut(s) 42, 302
Mox20I TGGCCA 1 cut(s) 731
Mph1103I ATGCAT 1 cut(s) 867
MroI TCCGGA 1 cut(s) 757
MscI TGGCCA 1 cut(s) 731
MseI TTAA 2 cut(s) 188, 1078
MslI CAYNNNNRTG 2 cut(s) 414, 759
Msp20I TGGCCA 1 cut(s) 731
MspA1I CMGCKG 1 cut(s) 243
MspI CCGG 3 cut(s) 462, 758, 1027
MspR9I CCNGG 4 cut(s) 206, 462, 463, 587
MvaI CCWGG 2 cut(s) 206, 587
MvnI CGCG 2 cut(s) 84, 150
MwoI GCNNNNNNNGC 6 cut(s) 253, 403, 737, 746, 788, 802
NciI CCSGG 2 cut(s) 462, 463
NdeII GATC 7 cut(s) 179, 433, 715, 835, 889, 940, 1021
NlaIII CATG 7 cut(s) 251, 413, 454, 865, 869, 968, 1049
NlaIV GGNNCC 2 cut(s) 435, 717
NmeAIII GCCGAG 1 cut(s) 580
NsiI ATGCAT 1 cut(s) 867
NspI RCATGY 1 cut(s) 251
PagI TCATGA 1 cut(s) 409
PfeI GAWTC 3 cut(s) 377, 582, 668
PfoI TCCNGGA 2 cut(s) 204, 585
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 67
Psp124BI GAGCTC 1 cut(s) 204
Psp1406I AACGTT 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 204, 585
PspGI CCWGG 2 cut(s) 204, 585
PspN4I GGNNCC 2 cut(s) 435, 717
PspPI GGNCC 2 cut(s) 67, 844
PspPPI RGGWCCY 1 cut(s) 67
PstI CTGCAG 2 cut(s) 751, 823
PsuI RGATCY 3 cut(s) 433, 715, 835
PvuII CAGCTG 1 cut(s) 243
RsaI GTAC 3 cut(s) 899, 983, 1064
RsaNI GTAC 3 cut(s) 898, 982, 1063
RseI CAYNNNNRTG 2 cut(s) 414, 759
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 2 cut(s) 188, 1078
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 7 cut(s) 179, 433, 715, 835, 889, 940, 1021
Sau96I GGNCC 2 cut(s) 67, 844
ScaI AGTACT 3 cut(s) 899, 983, 1064
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 4 cut(s) 206, 462, 463, 587
SduI GDGCHC 3 cut(s) 204, 399, 692
SfaNI GCATC 5 cut(s) 205, 265, 415, 696, 751
SfcI CTRYAG 2 cut(s) 747, 819
SinI GGWCC 2 cut(s) 67, 844
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 2 cut(s) 414, 759
SmlI CTYRAG 1 cut(s) 777
SmoI CTYRAG 1 cut(s) 777
Sse9I AATT 3 cut(s) 185, 290, 872
SsiI CCGC 4 cut(s) 148, 156, 469, 573
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 4 cut(s) 204, 460, 461, 585
TaaI ACNGT 1 cut(s) 814
TaiI ACGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 135, 182
TasI AATT 3 cut(s) 185, 290, 872
TatI WGTACW 3 cut(s) 897, 981, 1062
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 3 cut(s) 377, 582, 668
Tru1I TTAA 2 cut(s) 188, 1078
Tru9I TTAA 2 cut(s) 188, 1078
TscAI CASTG 2 cut(s) 739, 950
TspDTI ATGAA 2 cut(s) 249, 303
TspMI CCCGGG 1 cut(s) 461
TspRI CASTG 2 cut(s) 739, 950
VpaK11BI GGWCC 2 cut(s) 67, 844
XapI RAATTY 1 cut(s) 872
XceI RCATGY 1 cut(s) 251
XmaI CCCGGG 1 cut(s) 461
ZrmI AGTACT 3 cut(s) 899, 983, 1064
Zsp2I ATGCAT 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.