MD04G1054200.v1.1

Coiled-coil domain containing protein (DUF2052)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006653
Physical Location & Seq
Reverse (-)
6436888 .. 6439943
3056 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1054200.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGAGGGAAAGGCAGTGAGAGGCGAAGCGATGGAGAGCATATCGGAGAGGCTATCGTCGTTGGAGGATCTCTACTTCCCTCGTGCCCTACAATCCAACGCCCTCAATCCCTCTGAACGCAAGGCTATACTCCTCGACCTCCTTTCGAGGGACGTCGCCGTTTTCCTAGAACGTTATGGTTCAAAATTGACCTTTGATGAGCTCCGAGAGTTTGATGCGCTCCAAGATGATTATGAAATCAATTGGCATGTGAAGCATCTTAAAAGCTTAATGAGTCCAACATCAGATGAATTGAAGTCAAGGTCAGTTACAGTCAAGAACCGGAGGCGAGCATACCTGAATAAATTGATGTTTGACGGGAATTATTTCTCAGAGGACTCAATGAGAGAGAGAGAGCCATACTTGCATCATGAATATGTTGGGAAGTTTCAGGATCCCATTGGAAGGACCATGGCCAGACCCGGGGAAAGGTGGTCGGACATGTTGTTGAGGAGGGCAGAGGAAGCAGTTTTGATTTCAAAGATTAGAGGGGAGCAGCAGAGGTTGGGTGTTCCTGAAAGTGATTGGGTTGGTGGTGTTGATAGAAACCAGCAAGAAGAGCAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAGGAAGAGGATGAAGACGAAGAGGAAGCTGTGAGGGCTGACAGAGGCGCACAATCTACCGAGATGCTTATCGATCATCCAGTTGCACCCAACGGTGCTCAAACTACGGATAATCGAGATGTGCCCAATGGTGCTCAAACTGCAGGTCAGTCAGATGGGCCCAACGGTACTCAAACCACCACAGGAGGGCACAGTGAGAAAGAAACTTTATCTGCTGCGGAGTTGCAAGATCAGATGGACCAGTTCACCCACATCATGCAGCAAAAGTTTTTGTTGGGAGAAGATTCCGAGTACTTAGATTACACACAAATTGACAATGACGAAACACTGGATGATCACTGGCAGAGGGAAGCCAACCATGATGCTGAGGAGAAGTACTTTGCCGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2052

Protein Analysis

344

Amino Acids

39.58

Weight (kDa)

4.43

Isoelectric Point (pI)

62.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCD97-like_C PF09747 107 - 343 3e-51 Coiled-coil domain containing protein 97-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 156
Acc36I ACCTGC 1 cut(s) 773
AciI CCGC 1 cut(s) 857
AclI AACGTT 1 cut(s) 172
AclWI GGATC 3 cut(s) 75, 428, 441
AcoI YGGCCR 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 808, 932, 1016
AfiI CCNNNNNNNGG 5 cut(s) 148, 444, 462, 468, 825
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 3 cut(s) 183, 295, 519
AluBI AGCT 3 cut(s) 202, 267, 668
AluI AGCT 3 cut(s) 202, 267, 668
Alw21I GWGCWC 3 cut(s) 204, 739, 775
AlwI GGATC 3 cut(s) 75, 428, 441
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 2 cut(s) 453, 797
ApaI GGGCCC 1 cut(s) 801
ApeKI GCWGC 3 cut(s) 535, 854, 898
ArsI GACNNNNNNTTYG 2 cut(s) 769, 801
Asp700I GAANNNNTTC 1 cut(s) 365
AspLEI GCGC 2 cut(s) 220, 689
AspS9I GGNCC 4 cut(s) 447, 797, 798, 877
AsuC2I CCSGG 2 cut(s) 462, 463
AsuHPI GGTGA 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 2 cut(s) 447, 877
BaeGI GKGCMC 4 cut(s) 88, 765, 801, 831
BalI TGGCCA 1 cut(s) 455
BamHI GGATCC 1 cut(s) 433
BanII GRGCYC 2 cut(s) 204, 801
BauI CACGAG 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 660
Bbv12I GWGCWC 3 cut(s) 204, 739, 775
BbvCI CCTCAGC 1 cut(s) 1005
BbvI GCAGC 3 cut(s) 547, 841, 910
BccI CCATC 3 cut(s) 25, 788, 868
BceAI ACGGC 1 cut(s) 143
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 2 cut(s) 462, 463
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 780
BfuAI ACCTGC 1 cut(s) 773
BisI GCNGC 3 cut(s) 536, 855, 899
BlsI GCNGC 3 cut(s) 537, 856, 900
BmcAI AGTACT 2 cut(s) 932, 1016
Bme1390I CCNGG 2 cut(s) 462, 463
Bme18I GGWCC 2 cut(s) 447, 877
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 4 cut(s) 447, 797, 798, 877
BmiI GGNNCC 2 cut(s) 435, 799
BmrFI CCNGG 2 cut(s) 462, 463
BmsI GCATC 5 cut(s) 205, 265, 415, 693, 991
BpiI GAAGAC 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 1005
BpuMI CCSGG 2 cut(s) 462, 463
Bsa29I ATCGAT 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 707
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 4 cut(s) 450, 461, 462, 1023
BsaWI WCCGGW 1 cut(s) 321
Bsc4I CCNNNNNNNGG 5 cut(s) 148, 444, 462, 468, 825
Bse1I ACTGG 4 cut(s) 719, 880, 972, 983
Bse8I GATNNNNATC 1 cut(s) 707
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 4 cut(s) 450, 461, 462, 1023
BseGI GGATG 3 cut(s) 655, 715, 976
BseJI GATNNNNATC 1 cut(s) 707
BseLI CCNNNNNNNGG 5 cut(s) 148, 444, 462, 468, 825
BseMII CTCAG 2 cut(s) 384, 996
BseNI ACTGG 4 cut(s) 719, 880, 972, 983
BseSI GKGCMC 4 cut(s) 88, 765, 801, 831
BseXI GCAGC 3 cut(s) 547, 841, 910
BshFI GGCC 2 cut(s) 455, 799
BshVI ATCGAT 1 cut(s) 711
BsiHKAI GWGCWC 3 cut(s) 204, 739, 775
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 2 cut(s) 322, 462
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 5 cut(s) 148, 444, 462, 468, 825
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 2 cut(s) 455, 799
BsoBI CYCGRG 1 cut(s) 461
Bsp120I GGGCCC 1 cut(s) 797
Bsp1286I GDGCHC 7 cut(s) 88, 204, 739, 765, 775, 801, 831
Bsp143I GATC 5 cut(s) 67, 433, 712, 868, 973
Bsp19I CCATGG 1 cut(s) 450
BspACI CCGC 1 cut(s) 857
BspANI GGCC 2 cut(s) 455, 799
BspCNI CTCAG 2 cut(s) 383, 997
BspDI ATCGAT 1 cut(s) 711
BspHI TCATGA 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 435, 799
BspMAI CTGCAG 1 cut(s) 784
BspMI ACCTGC 1 cut(s) 773
BspPI GGATC 3 cut(s) 75, 428, 441
BspQI GCTCTTC 1 cut(s) 591
BsrI ACTGG 4 cut(s) 719, 880, 972, 983
BssECI CCNNGG 4 cut(s) 450, 461, 462, 1023
BssMI GATC 5 cut(s) 67, 433, 712, 868, 973
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 81
Bst4CI ACNGT 4 cut(s) 313, 734, 806, 833
Bst6I CTCTTC 4 cut(s) 591, 633, 639, 654
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 3 cut(s) 370, 934, 1005
BstDSI CCRYGG 1 cut(s) 450
BstF5I GGATG 3 cut(s) 655, 715, 976
BstHHI GCGC 2 cut(s) 220, 689
BstKTI GATC 5 cut(s) 70, 436, 715, 871, 976
BstMBI GATC 5 cut(s) 67, 433, 712, 868, 973
BstMWI GCNNNNNNNGC 6 cut(s) 253, 403, 503, 598, 674, 779
BstNSI RCATGY 2 cut(s) 251, 484
BstSCI CCNGG 2 cut(s) 460, 461
BstSFI CTRYAG 1 cut(s) 780
BstSLI GKGCMC 4 cut(s) 88, 765, 801, 831
BstV1I GCAGC 3 cut(s) 547, 841, 910
BstV2I GAAGAC 1 cut(s) 660
BstX2I RGATCY 2 cut(s) 67, 433
BstYI RGATCY 2 cut(s) 67, 433
Bsu15I ATCGAT 1 cut(s) 711
BsuRI GGCC 2 cut(s) 455, 799
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 44
BtsCI GGATG 3 cut(s) 655, 715, 976
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 4 cut(s) 21, 838, 965, 976
BveI ACCTGC 1 cut(s) 773
Cac8I GCNNGC 1 cut(s) 330
CciI TCATGA 1 cut(s) 409
CfoI GCGC 2 cut(s) 220, 689
Cfr13I GGNCC 4 cut(s) 447, 797, 798, 877
Cfr9I CCCGGG 1 cut(s) 461
ClaI ATCGAT 1 cut(s) 711
Csp6I GTAC 3 cut(s) 807, 931, 1015
CviAII CATG 6 cut(s) 248, 410, 451, 481, 895, 998
CviQI GTAC 3 cut(s) 807, 931, 1015
DdeI CTNAG 3 cut(s) 370, 934, 1005
DpnI GATC 5 cut(s) 69, 435, 714, 870, 975
DpnII GATC 5 cut(s) 67, 433, 712, 868, 973
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 4 cut(s) 591, 633, 639, 654
EarI CTCTTC 4 cut(s) 591, 633, 639, 654
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 2 cut(s) 204, 801
Eco47I GGWCC 2 cut(s) 447, 877
Eco53kI GAGCTC 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 2 cut(s) 204, 801
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 6 cut(s) 251, 413, 454, 484, 898, 1001
FaqI GGGAC 1 cut(s) 164
FatI CATG 6 cut(s) 247, 409, 450, 480, 894, 997
FbaI TGATCA 1 cut(s) 973
Fnu4HI GCNGC 3 cut(s) 536, 855, 899
FokI GGATG 3 cut(s) 662, 702, 983
FriOI GRGCYC 2 cut(s) 204, 801
Fsp4HI GCNGC 3 cut(s) 536, 855, 899
FspBI CTAG 1 cut(s) 167
GlaI GCGC 2 cut(s) 219, 688
GluI GCNGC 3 cut(s) 536, 855, 899
HaeIII GGCC 2 cut(s) 455, 799
HapII CCGG 2 cut(s) 322, 462
HhaI GCGC 2 cut(s) 220, 689
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 6 cut(s) 251, 413, 454, 484, 898, 1001
Hin6I GCGC 2 cut(s) 218, 687
HinP1I GCGC 2 cut(s) 218, 687
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 3 cut(s) 274, 377, 923
HpaII CCGG 2 cut(s) 322, 462
HphI GGTGA 1 cut(s) 877
Hpy166II GTNNAC 1 cut(s) 885
Hpy188I TCNGA 9 cut(s) 46, 115, 206, 286, 373, 478, 793, 873, 928
Hpy188III TCNNGA 5 cut(s) 316, 410, 431, 554, 755
Hpy8I GTNNAC 1 cut(s) 885
Hpy99I CGWCG 2 cut(s) 61, 158
HpyAV CCTTC 1 cut(s) 438
HpyCH4III ACNGT 4 cut(s) 313, 734, 806, 833
HpyCH4IV ACGT 2 cut(s) 153, 172
HpyCH4V TGCA 5 cut(s) 406, 725, 782, 865, 898
HpyF10VI GCNNNNNNNGC 6 cut(s) 253, 403, 503, 598, 674, 779
HpyF3I CTNAG 3 cut(s) 370, 934, 1005
HpySE526I ACGT 2 cut(s) 153, 172
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 6 cut(s) 251, 413, 454, 484, 898, 1001
HspAI GCGC 2 cut(s) 218, 687
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 5 cut(s) 67, 433, 712, 868, 973
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 3 cut(s) 207, 225, 532
Lsp1109I GCAGC 3 cut(s) 547, 841, 910
LweI GCATC 5 cut(s) 205, 265, 415, 693, 991
MaeI CTAG 1 cut(s) 167
MaeII ACGT 2 cut(s) 153, 172
MaeIII GTNAC 1 cut(s) 307
MalI GATC 5 cut(s) 69, 435, 714, 870, 975
MboI GATC 5 cut(s) 67, 433, 712, 868, 973
MboII GAAGA 8 cut(s) 608, 644, 647, 650, 656, 665, 671, 932
MfeI CAATTG 1 cut(s) 241
MflI RGATCY 2 cut(s) 67, 433
MhlI GDGCHC 7 cut(s) 88, 204, 739, 765, 775, 801, 831
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 6 cut(s) 185, 241, 290, 344, 361, 948
MluNI TGGCCA 1 cut(s) 455
MlyI GAGTC 2 cut(s) 283, 371
MmeI TCCRAC 4 cut(s) 42, 120, 302, 456
Mox20I TGGCCA 1 cut(s) 455
MroXI GAANNNNTTC 1 cut(s) 365
MscI TGGCCA 1 cut(s) 455
MseI TTAA 3 cut(s) 261, 269, 1030
MslI CAYNNNNRTG 1 cut(s) 414
Msp20I TGGCCA 1 cut(s) 455
MspI CCGG 2 cut(s) 322, 462
MspR9I CCNGG 2 cut(s) 462, 463
MunI CAATTG 1 cut(s) 241
MwoI GCNNNNNNNGC 6 cut(s) 253, 403, 503, 598, 674, 779
NciI CCSGG 2 cut(s) 462, 463
NcoI CCATGG 1 cut(s) 450
NdeII GATC 5 cut(s) 67, 433, 712, 868, 973
NlaIII CATG 6 cut(s) 251, 413, 454, 484, 898, 1001
NlaIV GGNNCC 2 cut(s) 435, 799
NspI RCATGY 2 cut(s) 251, 484
PagI TCATGA 1 cut(s) 409
PciI ACATGT 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 591
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 923
PkrI GCNGC 3 cut(s) 537, 856, 900
PleI GAGTC 2 cut(s) 282, 371
PpsI GAGTC 2 cut(s) 282, 371
PscI ACATGT 1 cut(s) 480
Psp124BI GAGCTC 1 cut(s) 204
Psp1406I AACGTT 1 cut(s) 172
PspN4I GGNNCC 2 cut(s) 435, 799
PspOMI GGGCCC 1 cut(s) 797
PspPI GGNCC 4 cut(s) 447, 797, 798, 877
PstI CTGCAG 1 cut(s) 784
PsuI RGATCY 2 cut(s) 67, 433
RsaI GTAC 3 cut(s) 808, 932, 1016
RsaNI GTAC 3 cut(s) 807, 931, 1015
RseI CAYNNNNRTG 1 cut(s) 414
SacI GAGCTC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 3 cut(s) 261, 269, 1030
SatI GCNGC 3 cut(s) 536, 855, 899
Sau3AI GATC 5 cut(s) 67, 433, 712, 868, 973
Sau96I GGNCC 4 cut(s) 447, 797, 798, 877
ScaI AGTACT 2 cut(s) 932, 1016
SchI GAGTC 2 cut(s) 283, 371
ScrFI CCNGG 2 cut(s) 462, 463
SduI GDGCHC 7 cut(s) 88, 204, 739, 765, 775, 801, 831
SfaNI GCATC 5 cut(s) 205, 265, 415, 693, 991
SfcI CTRYAG 1 cut(s) 780
SinI GGWCC 2 cut(s) 447, 877
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 414
Sse9I AATT 6 cut(s) 185, 241, 290, 344, 361, 948
SsiI CCGC 1 cut(s) 857
SspMI CTAG 1 cut(s) 167
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 460, 461
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 4 cut(s) 313, 734, 806, 833
TaiI ACGT 2 cut(s) 156, 175
TaqI TCGA 4 cut(s) 135, 146, 711, 754
TasI AATT 6 cut(s) 185, 241, 290, 344, 361, 948
TatI WGTACW 2 cut(s) 930, 1014
TfiI GAWTC 1 cut(s) 923
Tru1I TTAA 3 cut(s) 261, 269, 1030
Tru9I TTAA 3 cut(s) 261, 269, 1030
TscAI CASTG 4 cut(s) 21, 838, 972, 983
TseI GCWGC 3 cut(s) 535, 854, 898
TspDTI ATGAA 4 cut(s) 249, 303, 426, 666
TspGWI ACGGA 1 cut(s) 761
TspMI CCCGGG 1 cut(s) 461
TspRI CASTG 4 cut(s) 21, 838, 972, 983
VpaK11BI GGWCC 2 cut(s) 447, 877
XceI RCATGY 2 cut(s) 251, 484
XmaI CCCGGG 1 cut(s) 461
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 1 cut(s) 167
ZraI GACGTC 1 cut(s) 154
ZrmI AGTACT 2 cut(s) 932, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.