RchiOBHm_Chr7g0206421

Coiled-coil domain containing protein (DUF2052)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006653
Physical Location & Seq
Forward (+)
24127714 .. 24130061
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18474

Sequence Viewer

Length: 1089 bp
ATGGAGACGAAGGTAGTGAGAAGCGAAGCGATAGAGAGCATATCGGACAGGCTATCGGCGTCGGAGGACCTCTACTTCCCTCGCGCCCTGCAATCCACTACCATCACCCCCTCTCAGCGCAAGGCCATTCTCCTCGACCTCCTATCACGCGACGCTGCCGTCTTCTTAGAACGTTATGGATCGAAATTAACATCTGAGGAGCTCCTGGAGTTTGATGCGCTGACAGATGACTATGAAGTCAGCTGGCATGTGAAGCATCTTAGAAGTTTATTGAGTCCGACATCGGATGAATTGAAATCAAGGGCAGTTCAAGTGAAGAACAGGAGGCGAGCCTACCTGAATAAGTTGATATTTGGTGGGCAGTATTTCTCGGAGGATTCAATGAGGGAGAGAGAGCCCTACTTGCATCATGAGTATGTGGGGAAGTTTCAGGATCCGAGTGGTAGGAGCATGGCAAGACCCGGGGAGCGGTGGTCAGATACTTTGTTGAGGAGGTCAGAGGAAGCGATTTTGGTAGCAAAGATTAGAGGGGAGCAACAGAGGTTGGGTGTTGCTGAGAGTGATTGGGTTGGCGGTGAGAGAATCCAGGAAGTAGAGGAACAGGAGGAGGAAGAAGAAGAGGAGGAAGAGGAGGAGGAAGAGGAAGAGGAAGAAGAAGAGGAGGAGGAGGAGGAAGAATCTAACAAGGCTAATGGGGGCACACAATCATCAGAGATGCTGGTGGATCCTCCTAATGTGGCCAGTGGTGCTGAAACTGCAGACCATACGGATGCGGCTAATGTTGCTCAAGCAACTACAGCAGGGCAGAGTGAGCAAGCAACTGTATCTGCAGAAGAGTTGCAAGATCTGATGGACCAGTTCACCTACATCATGCATGAGAAATTTTTGTTAGGAGAAGATCACGAGTACTTAGATTACTCAAAGATAGACAACGACGAGACATTGGATGATCACTGGCAGAGAGAAGCCAACCATGACGCTGAAGAGAAGTACTTTGATGACATTATTGATGAGGACGAGACATTGGATGATCACCGGCAGAGAGAAGTGAATCATGACGCTGAACAGAAGTACTTTGATGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2052

Protein Analysis

362

Amino Acids

41.9

Weight (kDa)

4.32

Isoelectric Point (pI)

74.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCD97-like_C PF09747 107 - 334 3.4e-53 Coiled-coil domain containing protein 97-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 158, 236
AccBSI CCGCTC 1 cut(s) 469
AccII CGCG 2 cut(s) 84, 150
AciI CCGC 3 cut(s) 469, 573, 773
AclI AACGTT 1 cut(s) 172
AclWI GGATC 5 cut(s) 187, 428, 441, 719, 732
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 1 cut(s) 881
AcuI CTGAAG 1 cut(s) 1002
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 3 cut(s) 908, 992, 1073
AfiI CCNNNNNNNGG 1 cut(s) 468
AgsI TTSAA 3 cut(s) 295, 311, 381
AjnI CCWGG 2 cut(s) 204, 585
AluBI AGCT 2 cut(s) 202, 243
AluI AGCT 2 cut(s) 202, 243
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 932, 1013
AlwI GGATC 5 cut(s) 187, 428, 441, 719, 732
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 2 cut(s) 123, 738
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 1 cut(s) 881
AspLEI GCGC 3 cut(s) 86, 120, 220
AspS9I GGNCC 2 cut(s) 67, 853
AsuC2I CCSGG 2 cut(s) 462, 463
AsuHPI GGTGA 4 cut(s) 97, 587, 853, 1025
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 2 cut(s) 67, 853
BaeGI GKGCMC 1 cut(s) 701
BalI TGGCCA 1 cut(s) 740
BamHI GGATCC 2 cut(s) 433, 724
BanII GRGCYC 2 cut(s) 204, 399
BauI CACGAG 1 cut(s) 902
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 142
BccI CCATC 2 cut(s) 110, 844
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 206, 587
BclI TGATCA 2 cut(s) 949, 1030
BcnI CCSGG 2 cut(s) 462, 463
BcoDI GTCTC 2 cut(s) 932, 1013
BfmI CTRYAG 3 cut(s) 756, 795, 828
BglII AGATCT 1 cut(s) 844
BisI GCNGC 2 cut(s) 156, 774
BlsI GCNGC 2 cut(s) 157, 775
BmcAI AGTACT 3 cut(s) 908, 992, 1073
Bme1390I CCNGG 4 cut(s) 206, 462, 463, 587
Bme18I GGWCC 2 cut(s) 67, 853
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 67, 853
BmiI GGNNCC 2 cut(s) 435, 726
BmrFI CCNGG 4 cut(s) 206, 462, 463, 587
BmsI GCATC 5 cut(s) 205, 265, 415, 705, 760
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 771
BpuMI CCSGG 2 cut(s) 462, 463
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 461, 462
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 468
Bse118I RCCGGY 1 cut(s) 1035
Bse1I ACTGG 3 cut(s) 741, 856, 959
BseBI CCWGG 2 cut(s) 206, 587
BseDI CCNNGG 2 cut(s) 461, 462
BseGI GGATG 4 cut(s) 292, 775, 952, 1033
BseLI CCNNNNNNNGG 1 cut(s) 468
BseMII CTCAG 3 cut(s) 128, 186, 546
BseNI ACTGG 3 cut(s) 741, 856, 959
BseSI GKGCMC 1 cut(s) 701
BseXI GCAGC 1 cut(s) 142
Bsh1236I CGCG 2 cut(s) 84, 150
BshFI GGCC 2 cut(s) 125, 740
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 2 cut(s) 462, 1036
BslI CCNNNNNNNGG 1 cut(s) 468
BsmAI GTCTC 2 cut(s) 932, 1013
BsnI GGCC 2 cut(s) 125, 740
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 3 cut(s) 204, 399, 701
Bsp143I GATC 7 cut(s) 179, 433, 724, 844, 898, 949, 1030
BspACI CCGC 3 cut(s) 469, 573, 773
BspANI GGCC 2 cut(s) 125, 740
BspCNI CTCAG 3 cut(s) 127, 187, 547
BspFNI CGCG 2 cut(s) 84, 150
BspHI TCATGA 2 cut(s) 409, 1054
BspLI GGNNCC 2 cut(s) 435, 726
BspMAI CTGCAG 2 cut(s) 760, 832
BspPI GGATC 5 cut(s) 187, 428, 441, 719, 732
BsrBI CCGCTC 1 cut(s) 469
BsrFI RCCGGY 1 cut(s) 1035
BsrI ACTGG 3 cut(s) 741, 856, 959
BssAI RCCGGY 1 cut(s) 1035
BssECI CCNNGG 2 cut(s) 461, 462
BssMI GATC 7 cut(s) 179, 433, 724, 844, 898, 949, 1030
BssNI GRCGYC 1 cut(s) 59
BssSI CACGAG 1 cut(s) 902
Bst2BI CACGAG 1 cut(s) 902
Bst2UI CCWGG 2 cut(s) 206, 587
Bst4CI ACNGT 1 cut(s) 823
Bst6I CTCTTC 7 cut(s) 612, 621, 633, 639, 651, 828, 978
BstACI GRCGYC 1 cut(s) 59
BstC8I GCNNGC 3 cut(s) 245, 330, 816
BstDEI CTNAG 6 cut(s) 114, 166, 195, 260, 555, 910
BstF5I GGATG 4 cut(s) 292, 775, 952, 1033
BstFNI CGCG 2 cut(s) 84, 150
BstHHI GCGC 3 cut(s) 86, 120, 220
BstKTI GATC 7 cut(s) 182, 436, 727, 847, 901, 952, 1033
BstMAI GTCTC 2 cut(s) 932, 1013
BstMBI GATC 7 cut(s) 179, 433, 724, 844, 898, 949, 1030
BstMWI GCNNNNNNNGC 7 cut(s) 253, 403, 746, 755, 782, 797, 811
BstNI CCWGG 2 cut(s) 206, 587
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 4 cut(s) 204, 460, 461, 585
BstSFI CTRYAG 3 cut(s) 756, 795, 828
BstSLI GKGCMC 1 cut(s) 701
BstUI CGCG 2 cut(s) 84, 150
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 3 cut(s) 433, 724, 844
BstYI RGATCY 3 cut(s) 433, 724, 844
BsuRI GGCC 2 cut(s) 125, 740
BtsCI GGATG 4 cut(s) 292, 775, 952, 1033
BtsIMutI CAGTG 2 cut(s) 748, 952
Cac8I GCNNGC 3 cut(s) 245, 330, 816
CciI TCATGA 2 cut(s) 409, 1054
CfoI GCGC 3 cut(s) 86, 120, 220
Cfr10I RCCGGY 1 cut(s) 1035
Cfr13I GGNCC 2 cut(s) 67, 853
Cfr9I CCCGGG 1 cut(s) 461
CseI GACGC 4 cut(s) 48, 161, 986, 1067
Csp6I GTAC 3 cut(s) 907, 991, 1072
CviAII CATG 7 cut(s) 248, 410, 451, 871, 875, 974, 1055
CviQI GTAC 3 cut(s) 907, 991, 1072
DdeI CTNAG 6 cut(s) 114, 166, 195, 260, 555, 910
DpnI GATC 7 cut(s) 181, 435, 726, 846, 900, 951, 1032
DpnII GATC 7 cut(s) 179, 433, 724, 844, 898, 949, 1030
DrdI GACNNNNNNGTC 2 cut(s) 158, 236
DseDI GACNNNNNNGTC 2 cut(s) 158, 236
EaeI YGGCCR 1 cut(s) 738
Eam1104I CTCTTC 7 cut(s) 612, 621, 633, 639, 651, 828, 978
EarI CTCTTC 7 cut(s) 612, 621, 633, 639, 651, 828, 978
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 2 cut(s) 204, 399
Eco47I GGWCC 2 cut(s) 67, 853
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 1002
Eco88I CYCGRG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 202
EcoO109I RGGNCCY 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 204, 585
EcoT22I ATGCAT 1 cut(s) 876
EcoT38I GRGCYC 2 cut(s) 204, 399
FaeI CATG 7 cut(s) 251, 413, 454, 874, 878, 977, 1058
FatI CATG 7 cut(s) 247, 409, 450, 870, 874, 973, 1054
FbaI TGATCA 2 cut(s) 949, 1030
Fnu4HI GCNGC 2 cut(s) 156, 774
FokI GGATG 4 cut(s) 299, 782, 959, 1040
FriOI GRGCYC 2 cut(s) 204, 399
Fsp4HI GCNGC 2 cut(s) 156, 774
GlaI GCGC 3 cut(s) 85, 119, 219
GluI GCNGC 2 cut(s) 156, 774
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 2 cut(s) 125, 740
HapII CCGG 2 cut(s) 462, 1036
HgaI GACGC 4 cut(s) 48, 161, 986, 1067
HhaI GCGC 3 cut(s) 86, 120, 220
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 7 cut(s) 251, 413, 454, 874, 878, 977, 1058
Hin6I GCGC 3 cut(s) 84, 118, 218
HinP1I GCGC 3 cut(s) 84, 118, 218
HinfI GANTC 5 cut(s) 274, 377, 582, 677, 1051
HpaII CCGG 2 cut(s) 462, 1036
HphI GGTGA 4 cut(s) 97, 587, 853, 1025
Hpy166II GTNNAC 1 cut(s) 861
Hpy188III TCNNGA 4 cut(s) 410, 431, 902, 1055
Hpy8I GTNNAC 1 cut(s) 861
Hpy99I CGWCG 3 cut(s) 64, 155, 938
HpyAV CCTTC 1 cut(s) 4
HpyCH4III ACNGT 1 cut(s) 823
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 6 cut(s) 91, 406, 758, 830, 841, 874
HpyF10VI GCNNNNNNNGC 7 cut(s) 253, 403, 746, 755, 782, 797, 811
HpyF3I CTNAG 6 cut(s) 114, 166, 195, 260, 555, 910
HpySE526I ACGT 1 cut(s) 172
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 7 cut(s) 251, 413, 454, 874, 878, 977, 1058
HspAI GCGC 3 cut(s) 84, 118, 218
Ksp22I TGATCA 2 cut(s) 949, 1030
Kzo9I GATC 7 cut(s) 179, 433, 724, 844, 898, 949, 1030
LmnI GCTCC 5 cut(s) 199, 207, 447, 466, 532
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 5 cut(s) 205, 265, 415, 705, 760
MaeII ACGT 1 cut(s) 172
MalI GATC 7 cut(s) 181, 435, 726, 846, 900, 951, 1032
MbiI CCGCTC 1 cut(s) 469
MboI GATC 7 cut(s) 179, 433, 724, 844, 898, 949, 1030
MflI RGATCY 3 cut(s) 433, 724, 844
MhlI GDGCHC 3 cut(s) 204, 399, 701
MlsI TGGCCA 1 cut(s) 740
MluCI AATT 3 cut(s) 185, 290, 881
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 2 cut(s) 42, 302
Mox20I TGGCCA 1 cut(s) 740
Mph1103I ATGCAT 1 cut(s) 876
MscI TGGCCA 1 cut(s) 740
MseI TTAA 2 cut(s) 188, 1087
MslI CAYNNNNRTG 2 cut(s) 414, 768
Msp20I TGGCCA 1 cut(s) 740
MspA1I CMGCKG 1 cut(s) 243
MspI CCGG 2 cut(s) 462, 1036
MspR9I CCNGG 4 cut(s) 206, 462, 463, 587
MvaI CCWGG 2 cut(s) 206, 587
MvnI CGCG 2 cut(s) 84, 150
MwoI GCNNNNNNNGC 7 cut(s) 253, 403, 746, 755, 782, 797, 811
NciI CCSGG 2 cut(s) 462, 463
NdeII GATC 7 cut(s) 179, 433, 724, 844, 898, 949, 1030
NlaIII CATG 7 cut(s) 251, 413, 454, 874, 878, 977, 1058
NlaIV GGNNCC 2 cut(s) 435, 726
NsiI ATGCAT 1 cut(s) 876
NspI RCATGY 1 cut(s) 251
PagI TCATGA 2 cut(s) 409, 1054
PfeI GAWTC 4 cut(s) 377, 582, 677, 1051
PfoI TCCNGGA 2 cut(s) 204, 585
PkrI GCNGC 2 cut(s) 157, 775
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 67
Psp124BI GAGCTC 1 cut(s) 204
Psp1406I AACGTT 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 204, 585
PspGI CCWGG 2 cut(s) 204, 585
PspN4I GGNNCC 2 cut(s) 435, 726
PspPI GGNCC 2 cut(s) 67, 853
PspPPI RGGWCCY 1 cut(s) 67
PstI CTGCAG 2 cut(s) 760, 832
PsuI RGATCY 3 cut(s) 433, 724, 844
PvuII CAGCTG 1 cut(s) 243
RsaI GTAC 3 cut(s) 908, 992, 1073
RsaNI GTAC 3 cut(s) 907, 991, 1072
RseI CAYNNNNRTG 2 cut(s) 414, 768
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 2 cut(s) 188, 1087
SatI GCNGC 2 cut(s) 156, 774
Sau3AI GATC 7 cut(s) 179, 433, 724, 844, 898, 949, 1030
Sau96I GGNCC 2 cut(s) 67, 853
ScaI AGTACT 3 cut(s) 908, 992, 1073
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 4 cut(s) 206, 462, 463, 587
SduI GDGCHC 3 cut(s) 204, 399, 701
SfaNI GCATC 5 cut(s) 205, 265, 415, 705, 760
SfcI CTRYAG 3 cut(s) 756, 795, 828
SinI GGWCC 2 cut(s) 67, 853
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 2 cut(s) 414, 768
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
Sse9I AATT 3 cut(s) 185, 290, 881
SsiI CCGC 3 cut(s) 469, 573, 773
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 4 cut(s) 204, 460, 461, 585
TaaI ACNGT 1 cut(s) 823
TaiI ACGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 135, 182
TasI AATT 3 cut(s) 185, 290, 881
TatI WGTACW 3 cut(s) 906, 990, 1071
TauI GCSGC 1 cut(s) 776
TfiI GAWTC 4 cut(s) 377, 582, 677, 1051
Tru1I TTAA 2 cut(s) 188, 1087
Tru9I TTAA 2 cut(s) 188, 1087
TscAI CASTG 2 cut(s) 748, 959
TseI GCWGC 1 cut(s) 155
TspDTI ATGAA 2 cut(s) 249, 303
TspGWI ACGGA 1 cut(s) 782
TspMI CCCGGG 1 cut(s) 461
TspRI CASTG 2 cut(s) 748, 959
VpaK11BI GGWCC 2 cut(s) 67, 853
XapI RAATTY 1 cut(s) 881
XceI RCATGY 1 cut(s) 251
XmaI CCCGGG 1 cut(s) 461
ZrmI AGTACT 3 cut(s) 908, 992, 1073
Zsp2I ATGCAT 1 cut(s) 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.