Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0001361

Physical Location & Seq

Genomic Coordinates Forward (+)

13902522 .. 13910629

Gene Structure 8108 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g22570.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1662 bp

ATGGAGGAGTGTTCAACTGGGGCACTAGTCCCGGCAGTGAAAGTGGAACCATTTTTATCAAGCGTTGGGGGTTCAGTGGATCCATGCAGCAGTGACATGGGTCGTCTGACAGAGGAGAAAGGAATGTCAGAGGTGGACAAGGATTTGCTGTGTCCAATTTGTATGCAGATTGTAAAGGACGCATTCCTCACAGCGTGCGGTCATAGCTTCTGCTATATGTGCATCATCACTCACCTTAGCAATAAGAGTGACTGCCCTTGCTGTGCTCAATCCCTCTGCAAGAACCAATTGTTCCCAAATTTCTTACTGGACAAGCTTCTGAAGAAGACTTCGGCTCAACAAATTTCAAAAAGCGCATCCCCTGTGGAGCATTTTCGTCAGGCATTGCAGCAGGGCTGTGAAGTGTCCATCAAGGAGCTTGACACCTTATTGGCACTCCTTACAGAGAAGAAGAGGAAAATGGAACAAGAAGAAGCCGAGAGAAATATGCAAATATTGCTCGACTTCTTGAATTGCTTAAGGAAGCAGAAAGTTCAAGAGCTGAATGAGGTACAATCCCATCTTCAATTTCTTAAAGAAGACATAAGTGTAGTTGAGAGACGCAGAATGGACTTGTACCGTGCCAAGGATAGGTACTCTGTCAGGCTACGGACGCTTGGAGGAGATGATTCTTCCAATGGTGCAAGAAATTCATGGCCTTCCTCTATAGACAACAATTCCAGTGCTCTCAGAGTACGAGGAGGAACGTCCTCTTGGAATATTCAGGGACGAGGTTTGCAGAGGAATGATGCTTTAAGCGCATCAGACTCCCAGTTTATGAATCAATCGGGACTTGCTGTTGCCAGGAAAAAGCGGGTCCATGCATTGTTTGATGAGCTACAAGAGTGTTACCTGCAAAAGCGACGTCAGATGAACCAACCATATTCCCAGCTAGAACGGAACAGATCTATAATACCCAGAGAAGGTTATACTGCAGGTCTTGCCGATTTCCAATCAGTGCTTACTACATTAACACGCTACAGTCGAATGAGGGTCATTGCTGAACTTAGGCATGAGGATCTATTTCACTCGGCCAATATAGTATCAAGCATTGAATTTGACCGTGATTATGAGTTGTTCGCCACTGCTGGAGTTTCAAAGTGCATAAAAGTTTTTGACTTTTCCTCGGTTGTGAATGATCCAGCTGATATGCACACTGCCGTTGTGGAGATGCCTACACGATCAAAGCTGAGTTGCTTGAGCTGGAACAAGTTTACTAAAAACCATATAGCTAGTACTGATTATGAGGGAATAGTAACTGTTTGGGATGTAGATACTCGGCAGAGTATCATGGAATATGAAGAGCATGAGAAACGTGCTTGGAGTGTTGATTTTTCATGCACAGAACCCTCAATGCTTGTATCTGGAAGTGATGATTGTAAGGTAAAGGTTTGGTGCACAAGGCAGGAAGCTAGTGTTCTTAACATTGACATGAAAGCGAACATATGTTGCGTGAAGTATAATCCTGGATCGAGCAACTGCATTGCGGTTGGTTCAGCAGATCATCATATTCACTATTATGATTTAAGAAATGTGAGTCAACCACTGCATGTGTTCACTGGGCACAGGAAAACTGTTTCGTTCGAACTTTTAAAGGTCATACAAATGAGAAGAACTTCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009791 GO:0009812 GO:0009987 GO:0010119 GO:0010228 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40 zf-C3HC4_2

Protein Analysis

554

Amino Acids

62.68

Weight (kDa)

7.47

Isoelectric Point (pI)

55.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_2	PF13923	51 - 88	5.5e-10	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	51 - 88	1.7e-08	Zinc finger, C3HC4 type (RING finger)
WDR55	PF24796	355 - 540	1e-09	WDR55
Beta-prop_WDR5	PF25175	358 - 487	1.3e-13	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	358 - 484	1.8e-10	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	358 - 441	3e-07	RIG second beta-propeller
WD40_CDC20-Fz	PF24807	402 - 539	1.4e-14	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	407 - 526	1.8e-17	THOC3 beta-propeller domain
WD40_Prp19	PF24814	409 - 485	1.2e-07	Prp19 WD40 domain
WD40_Gbeta	PF25391	410 - 532	2.6e-06	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	419 - 539	4.6e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	420 - 540	2.1e-14	WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	420 - 530	2.5e-06	MABP1/WDR62 second WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32950 AT2G32950
fragaria_vesca	FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_5g22570 FvH4_5g22570
malus_domestica	MD06G1037700.v1.1 MD10G1328500.v1.1 MD15G1405100.v1.1 MD15G1405200.v1.1 MD16G1272200.v1.1
prunus_persica	Prupe.4G013500_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1
pyrus_communis	pycom10g27830 pycom16g24110
rosa_chinensis	RchiOBHm_Chr5g0001791 RchiOBHm_Chr7g0210341
rosa_laevigata	RLG00000003045 RLG00000030973
rosa_multiflora	Rmu_co8137394.1_g000001 Rmu_sc0003807.1_g000002 Rmu_ssc0000237.1_g000012
rosa_roxburghii	Rroxscaffold_1G00071250 Rroxscaffold_1G00074540 Rroxscaffold_3G00248380
rosa_rugosa	Rorug04G0393100 Rorug04G0393200 Rorug04G0393300 Rorug07G0121200 Rorug07G0121200 Rorug07G0121300
rosa_samantha	Rh5AG014400 Rh5BG017800 Rh5CG015500 Rh5DG015900 Rh7AG253900 Rh7BG248800 Rh7CG270700 Rh7DG261400
rosa_wichuraiana	Rw5G001390 Rw7G021640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	907
Acc36I	ACCTGC	2 cut(s)	900, 965
AciI	CCGC	3 cut(s)	198, 853, 1526
AclWI	GGATC	5 cut(s)	74, 87, 1065, 1172, 1516
AcoI	YGGCCR	1 cut(s)	1071
AcsI	RAATTY	4 cut(s)	298, 342, 688, 1094
AcuI	CTGAAG	1 cut(s)	341
AcyI	GRCGYC	1 cut(s)	904
AdeI	CACNNNGTG	1 cut(s)	195
AfaI	GTAC	5 cut(s)	552, 617, 635, 735, 1276
AfiI	CCNNNNNNNGG	3 cut(s)	625, 630, 962
AflII	CTTAAG	1 cut(s)	517
AgsI	TTSAA	7 cut(s)	15, 348, 511, 536, 566, 1094, 1137
AhlI	ACTAGT	1 cut(s)	25
AjnI	CCWGG	2 cut(s)	842, 1504
AjuI	GAANNNNNNNTTGG	2 cut(s)	736, 768
Alw21I	GWGCWC	3 cut(s)	268, 727, 1439
Alw26I	GTCTC	1 cut(s)	592
Alw44I	GTGCAC	1 cut(s)	1435
AlwI	GGATC	5 cut(s)	74, 87, 1065, 1172, 1516
AoxI	GGCC	2 cut(s)	695, 1071
ApaLI	GTGCAC	1 cut(s)	1435
ApeKI	GCWGC	2 cut(s)	87, 388
ApoI	RAATTY	4 cut(s)	298, 342, 688, 1094
ArsI	GACNNNNNNTTYG	2 cut(s)	889, 921
Asp700I	GAANNNNTTC	1 cut(s)	1654
AspLEI	GCGC	2 cut(s)	356, 800
AspS9I	GGNCC	1 cut(s)	856
AsuC2I	CCSGG	1 cut(s)	32
AsuHPI	GGTGA	1 cut(s)	224
AsuII	TTCGAA	1 cut(s)	1623
AvaII	GGWCC	1 cut(s)	856
BaeGI	GKGCMC	3 cut(s)	25, 1439, 1605
BamHI	GGATCC	1 cut(s)	79
BbsI	GAAGAC	2 cut(s)	332, 585
Bbv12I	GWGCWC	3 cut(s)	268, 727, 1439
BbvI	GCAGC	2 cut(s)	99, 400
BccI	CCATC	2 cut(s)	416, 567
BceAI	ACGGC	1 cut(s)	1184
BciT130I	CCWGG	2 cut(s)	844, 1506
BcnI	CCSGG	1 cut(s)	32
BcoDI	GTCTC	1 cut(s)	592
BcuI	ACTAGT	1 cut(s)	25
BfaI	CTAG	4 cut(s)	26, 932, 1272, 1452
BfmI	CTRYAG	3 cut(s)	705, 972, 1018
BfrI	CTTAAG	1 cut(s)	517
BfuAI	ACCTGC	2 cut(s)	900, 965
BglII	AGATCT	1 cut(s)	944
BisI	GCNGC	2 cut(s)	88, 389
BlsI	GCNGC	2 cut(s)	89, 390
BmcAI	AGTACT	1 cut(s)	1276
Bme1390I	CCNGG	3 cut(s)	32, 844, 1506
Bme18I	GGWCC	1 cut(s)	856
BmgT120I	GGNCC	1 cut(s)	856
BmiI	GGNNCC	3 cut(s)	48, 81, 857
BmrFI	CCNGG	3 cut(s)	32, 844, 1506
BmrI	ACTGGG	3 cut(s)	27, 805, 1608
BmsI	GCATC	5 cut(s)	231, 365, 778, 809, 1200
BmuI	ACTGGG	3 cut(s)	27, 805, 1608
BoxI	GACNNNNGTC	1 cut(s)	99
BpiI	GAAGAC	2 cut(s)	332, 585
BpmI	CTGGAG	1 cut(s)	1149
Bpu10I	CCTNAGC	1 cut(s)	236
Bpu14I	TTCGAA	1 cut(s)	1623
BpuEI	CTTGAG	1 cut(s)	1258
BpuMI	CCSGG	1 cut(s)	32
BsaHI	GRCGYC	1 cut(s)	904
BsaJI	CCNNGG	2 cut(s)	624, 1164
Bsc4I	CCNNNNNNNGG	3 cut(s)	625, 630, 962
Bse1I	ACTGG	5 cut(s)	22, 312, 720, 811, 1603
Bse3DI	GCAATG	3 cut(s)	383, 1035, 1521
BseBI	CCWGG	2 cut(s)	844, 1506
BseDI	CCNNGG	2 cut(s)	624, 1164
BseGI	GGATG	2 cut(s)	356, 1312
BseLI	CCNNNNNNNGG	3 cut(s)	625, 630, 962
BseMI	GCAATG	3 cut(s)	383, 1035, 1521
BseMII	CTCAG	2 cut(s)	742, 1220
BseNI	ACTGG	5 cut(s)	22, 312, 720, 811, 1603
BseRI	GAGGAG	4 cut(s)	20, 128, 675, 753
BseSI	GKGCMC	3 cut(s)	25, 1439, 1605
BseXI	GCAGC	2 cut(s)	99, 400
BseYI	CCCAGC	1 cut(s)	927
BshFI	GGCC	2 cut(s)	697, 1073
BsiHKAI	GWGCWC	3 cut(s)	268, 727, 1439
BsiSI	CCGG	1 cut(s)	32
BslFI	GGGAC	3 cut(s)	14, 780, 843
BslI	CCNNNNNNNGG	3 cut(s)	625, 630, 962
BsmAI	GTCTC	1 cut(s)	592
BsmBI	CGTCTC	1 cut(s)	592
BsmFI	GGGAC	3 cut(s)	14, 780, 843
BsmI	GAATGC	1 cut(s)	182
BsnI	GGCC	2 cut(s)	697, 1073
Bsp119I	TTCGAA	1 cut(s)	1623
Bsp1286I	GDGCHC	5 cut(s)	25, 268, 727, 1439, 1605
Bsp143I	GATC	7 cut(s)	79, 944, 1057, 1177, 1220, 1508, 1540
BspACI	CCGC	3 cut(s)	198, 853, 1526
BspANI	GGCC	2 cut(s)	697, 1073
BspCNI	CTCAG	2 cut(s)	741, 1221
BspLI	GGNNCC	3 cut(s)	48, 81, 857
BspMAI	CTGCAG	1 cut(s)	976
BspMI	ACCTGC	2 cut(s)	900, 965
BspPI	GGATC	5 cut(s)	74, 87, 1065, 1172, 1516
BspQI	GCTCTTC	1 cut(s)	1335
BspT104I	TTCGAA	1 cut(s)	1623
BspTI	CTTAAG	1 cut(s)	517
BsrDI	GCAATG	3 cut(s)	383, 1035, 1521
BsrI	ACTGG	5 cut(s)	22, 312, 720, 811, 1603
BssECI	CCNNGG	2 cut(s)	624, 1164
BssMI	GATC	7 cut(s)	79, 944, 1057, 1177, 1220, 1508, 1540
BssNI	GRCGYC	1 cut(s)	904
BssT1I	CCWWGG	1 cut(s)	624
Bst2UI	CCWGG	2 cut(s)	844, 1506
Bst4CI	ACNGT	5 cut(s)	620, 1022, 1103, 1300, 1615
Bst6I	CTCTTC	2 cut(s)	446, 1335
BstACI	GRCGYC	1 cut(s)	904
BstAFI	CTTAAG	1 cut(s)	517
BstAPI	GCANNNNNTGC	2 cut(s)	496, 980
BstBI	TTCGAA	1 cut(s)	1623
BstC8I	GCNNGC	1 cut(s)	196
BstDEI	CTNAG	4 cut(s)	236, 728, 1046, 1229
BstF5I	GGATG	2 cut(s)	356, 1312
BstHHI	GCGC	2 cut(s)	356, 800
BstKTI	GATC	7 cut(s)	82, 947, 1060, 1180, 1223, 1511, 1543
BstMAI	GTCTC	1 cut(s)	592
BstMBI	GATC	7 cut(s)	79, 944, 1057, 1177, 1220, 1508, 1540
BstMWI	GCNNNNNNNGC	6 cut(s)	204, 219, 496, 652, 797, 980
BstNI	CCWGG	2 cut(s)	844, 1506
BstNSI	RCATGY	1 cut(s)	1592
BstPAI	GACNNNNGTC	1 cut(s)	99
BstSCI	CCNGG	3 cut(s)	30, 842, 1504
BstSFI	CTRYAG	3 cut(s)	705, 972, 1018
BstSLI	GKGCMC	3 cut(s)	25, 1439, 1605
BstV1I	GCAGC	2 cut(s)	99, 400
BstV2I	GAAGAC	2 cut(s)	332, 585
BstX2I	RGATCY	3 cut(s)	79, 944, 1057
BstYI	RGATCY	3 cut(s)	79, 944, 1057
BsuRI	GGCC	2 cut(s)	697, 1073
BtsCI	GGATG	2 cut(s)	356, 1312
BtsI	GCAGTG	5 cut(s)	42, 97, 1122, 1194, 1583
BtsIMutI	CAGTG	9 cut(s)	42, 81, 97, 727, 1002, 1122, 1194, 1583, 1596
BveI	ACCTGC	2 cut(s)	900, 965
Cac8I	GCNNGC	1 cut(s)	196
CfoI	GCGC	2 cut(s)	356, 800
Cfr13I	GGNCC	1 cut(s)	856
CseI	GACGC	3 cut(s)	188, 609, 661
Csp6I	GTAC	5 cut(s)	551, 616, 634, 734, 1275
CviQI	GTAC	5 cut(s)	551, 616, 634, 734, 1275
DdeI	CTNAG	4 cut(s)	236, 728, 1046, 1229
DpnI	GATC	7 cut(s)	81, 946, 1059, 1179, 1222, 1510, 1542
DpnII	GATC	7 cut(s)	79, 944, 1057, 1177, 1220, 1508, 1540
DraI	TTTAAA	1 cut(s)	1632
DraIII	CACNNNGTG	1 cut(s)	195
EaeI	YGGCCR	1 cut(s)	1071
Eam1104I	CTCTTC	2 cut(s)	446, 1335
EarI	CTCTTC	2 cut(s)	446, 1335
Eco130I	CCWWGG	1 cut(s)	624
Eco47I	GGWCC	1 cut(s)	856
Eco57I	CTGAAG	1 cut(s)	341
EcoRII	CCWGG	2 cut(s)	842, 1504
EcoT14I	CCWWGG	1 cut(s)	624
EcoT22I	ATGCAT	1 cut(s)	865
ErhI	CCWWGG	1 cut(s)	624
Esp3I	CGTCTC	1 cut(s)	592
FaqI	GGGAC	3 cut(s)	14, 780, 843
FauI	CCCGC	1 cut(s)	846
FauNDI	CATATG	1 cut(s)	1484
Fnu4HI	GCNGC	2 cut(s)	88, 389
FokI	GGATG	2 cut(s)	343, 1319
Fsp4HI	GCNGC	2 cut(s)	88, 389
FspBI	CTAG	4 cut(s)	26, 932, 1272, 1452
GlaI	GCGC	2 cut(s)	355, 799
GluI	GCNGC	2 cut(s)	88, 389
GsaI	CCCAGC	1 cut(s)	931
GsuI	CTGGAG	1 cut(s)	1149
HaeIII	GGCC	2 cut(s)	697, 1073
HapII	CCGG	1 cut(s)	32
HgaI	GACGC	3 cut(s)	188, 609, 661
HhaI	GCGC	2 cut(s)	356, 800
Hin1I	GRCGYC	1 cut(s)	904
Hin6I	GCGC	2 cut(s)	354, 798
HinP1I	GCGC	2 cut(s)	354, 798
HincII	GTYRAC	1 cut(s)	1580
HindII	GTYRAC	1 cut(s)	1580
HindIII	AAGCTT	1 cut(s)	314
HinfI	GANTC	4 cut(s)	668, 806, 820, 1576
HpaII	CCGG	1 cut(s)	32
HphI	GGTGA	1 cut(s)	224
Hpy166II	GTNNAC	5 cut(s)	136, 1254, 1437, 1580, 1596
Hpy188I	TCNGA	6 cut(s)	108, 130, 321, 731, 805, 909
Hpy188III	TCNNGA	4 cut(s)	508, 536, 828, 1404
Hpy8I	GTNNAC	5 cut(s)	136, 1254, 1437, 1580, 1596
Hpy99I	CGWCG	1 cut(s)	906
HpyAV	CCTTC	2 cut(s)	708, 956
HpyCH4III	ACNGT	5 cut(s)	620, 1022, 1103, 1300, 1615
HpyCH4IV	ACGT	3 cut(s)	746, 904, 1354
HpyF10VI	GCNNNNNNNGC	6 cut(s)	204, 219, 496, 652, 797, 980
HpyF3I	CTNAG	4 cut(s)	236, 728, 1046, 1229
HpySE526I	ACGT	3 cut(s)	746, 904, 1354
Hsp92I	GRCGYC	1 cut(s)	904
HspAI	GCGC	2 cut(s)	354, 798
Kzo9I	GATC	7 cut(s)	79, 944, 1057, 1177, 1220, 1508, 1540
LguI	GCTCTTC	1 cut(s)	1335
LmnI	GCTCC	2 cut(s)	367, 415
Lsp1109I	GCAGC	2 cut(s)	99, 400
LweI	GCATC	5 cut(s)	231, 365, 778, 809, 1200
MaeI	CTAG	4 cut(s)	26, 932, 1272, 1452
MaeII	ACGT	3 cut(s)	746, 904, 1354
MaeIII	GTNAC	4 cut(s)	92, 248, 887, 1294
MalI	GATC	7 cut(s)	81, 946, 1059, 1179, 1222, 1510, 1542
MboI	GATC	7 cut(s)	79, 944, 1057, 1177, 1220, 1508, 1540
MfeI	CAATTG	1 cut(s)	287
MflI	RGATCY	3 cut(s)	79, 944, 1057
MhlI	GDGCHC	5 cut(s)	25, 268, 727, 1439, 1605
MluCI	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 688, 715, 1094
MlyI	GAGTC	2 cut(s)	800, 1585
Mph1103I	ATGCAT	1 cut(s)	865
MroXI	GAANNNNTTC	1 cut(s)	1654
MseI	TTAA	7 cut(s)	518, 573, 794, 1010, 1461, 1565, 1631
MslI	CAYNNNNRTG	3 cut(s)	1469, 1557, 1643
MspA1I	CMGCKG	1 cut(s)	1184
MspCI	CTTAAG	1 cut(s)	517
MspI	CCGG	1 cut(s)	32
MspR9I	CCNGG	3 cut(s)	32, 844, 1506
MunI	CAATTG	1 cut(s)	287
Mva1269I	GAATGC	1 cut(s)	182
MvaI	CCWGG	2 cut(s)	844, 1506
MwoI	GCNNNNNNNGC	6 cut(s)	204, 219, 496, 652, 797, 980
NciI	CCSGG	1 cut(s)	32
NdeI	CATATG	1 cut(s)	1484
NdeII	GATC	7 cut(s)	79, 944, 1057, 1177, 1220, 1508, 1540
NlaIV	GGNNCC	3 cut(s)	48, 81, 857
NmeAIII	GCCGAG	3 cut(s)	502, 1049, 1297
NmuCI	GTSAC	2 cut(s)	92, 248
NsiI	ATGCAT	1 cut(s)	865
NspI	RCATGY	1 cut(s)	1592
NspV	TTCGAA	1 cut(s)	1623
PciSI	GCTCTTC	1 cut(s)	1335
PcsI	WCGNNNNNNNCGW	1 cut(s)	1021
PctI	GAATGC	1 cut(s)	182
PdmI	GAANNNNTTC	1 cut(s)	1654
PfeI	GAWTC	2 cut(s)	668, 820
PfoI	TCCNGGA	1 cut(s)	1504
PkrI	GCNGC	2 cut(s)	89, 390
PleI	GAGTC	2 cut(s)	800, 1584
PpsI	GAGTC	2 cut(s)	800, 1584
PshAI	GACNNNNGTC	1 cut(s)	99
Psp6I	CCWGG	2 cut(s)	842, 1504
PspFI	CCCAGC	1 cut(s)	927
PspGI	CCWGG	2 cut(s)	842, 1504
PspN4I	GGNNCC	3 cut(s)	48, 81, 857
PspPI	GGNCC	1 cut(s)	856
PstI	CTGCAG	1 cut(s)	976
PsuI	RGATCY	3 cut(s)	79, 944, 1057
PvuII	CAGCTG	1 cut(s)	1184
RsaI	GTAC	5 cut(s)	552, 617, 635, 735, 1276
RsaNI	GTAC	5 cut(s)	551, 616, 634, 734, 1275
RseI	CAYNNNNRTG	3 cut(s)	1469, 1557, 1643
SapI	GCTCTTC	1 cut(s)	1335
SaqAI	TTAA	7 cut(s)	518, 573, 794, 1010, 1461, 1565, 1631
SatI	GCNGC	2 cut(s)	88, 389
Sau3AI	GATC	7 cut(s)	79, 944, 1057, 1177, 1220, 1508, 1540
Sau96I	GGNCC	1 cut(s)	856
ScaI	AGTACT	1 cut(s)	1276
SchI	GAGTC	2 cut(s)	800, 1585
ScrFI	CCNGG	3 cut(s)	32, 844, 1506
SduI	GDGCHC	5 cut(s)	25, 268, 727, 1439, 1605
SfaNI	GCATC	5 cut(s)	231, 365, 778, 809, 1200
SfcI	CTRYAG	3 cut(s)	705, 972, 1018
SfuI	TTCGAA	1 cut(s)	1623
SinI	GGWCC	1 cut(s)	856
SmiMI	CAYNNNNRTG	3 cut(s)	1469, 1557, 1643
SmlI	CTYRAG	2 cut(s)	517, 1237
SmoI	CTYRAG	2 cut(s)	517, 1237
SpeI	ACTAGT	1 cut(s)	25
Sse9I	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 688, 715, 1094
SsiI	CCGC	3 cut(s)	198, 853, 1526
SspI	AATATT	2 cut(s)	495, 760
SspMI	CTAG	4 cut(s)	26, 932, 1272, 1452
StyD4I	CCNGG	3 cut(s)	30, 842, 1504
StyI	CCWWGG	1 cut(s)	624
TaaI	ACNGT	5 cut(s)	620, 1022, 1103, 1300, 1615
TaiI	ACGT	3 cut(s)	749, 907, 1357
TaqI	TCGA	4 cut(s)	501, 1024, 1511, 1623
TasI	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 688, 715, 1094
TatI	WGTACW	1 cut(s)	1274
TfiI	GAWTC	2 cut(s)	668, 820
Tru1I	TTAA	7 cut(s)	518, 573, 794, 1010, 1461, 1565, 1631
Tru9I	TTAA	7 cut(s)	518, 573, 794, 1010, 1461, 1565, 1631
TscAI	CASTG	9 cut(s)	42, 81, 97, 727, 1002, 1129, 1201, 1590, 1603
TseFI	GTSAC	2 cut(s)	92, 248
TseI	GCWGC	2 cut(s)	87, 388
Tsp45I	GTSAC	2 cut(s)	92, 248
TspDTI	ATGAA	6 cut(s)	681, 833, 926, 1353, 1365, 1487
TspGWI	ACGGA	2 cut(s)	664, 952
TspRI	CASTG	9 cut(s)	42, 81, 97, 727, 1002, 1129, 1201, 1590, 1603
Vha464I	CTTAAG	1 cut(s)	517
VneI	GTGCAC	1 cut(s)	1435
VpaK11BI	GGWCC	1 cut(s)	856
XapI	RAATTY	4 cut(s)	298, 342, 688, 1094
XceI	RCATGY	1 cut(s)	1592
XmnI	GAANNNNTTC	1 cut(s)	1654
XspI	CTAG	4 cut(s)	26, 932, 1272, 1452
ZraI	GACGTC	1 cut(s)	905
ZrmI	AGTACT	1 cut(s)	1276
Zsp2I	ATGCAT	1 cut(s)	865

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.