Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0001361

Physical Location & Seq

Genomic Coordinates Forward (+)

1292629 .. 1296460

Gene Structure 3832 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG017800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1926 bp

ATGGGTGGAGGACCAATGTTCCCGACCGTGGCGAAACTGGAGCTAATGGCGGCAGACTCATCGTCGGCGGAAACGGCGGCGTTTCAGAAGGAGGCTCCGCCGGACAAGGACTTGATGTGTCCGATATGCATACAGGTGATAAGGGACGCGTTCCTCACTGCTTGCGGCCATAGCTTCTGCCACAGCTGCATAGTCACTCATCTCGGAATCAAGAGCGACTGTCCTTGCTGTGGTCAATACCTCACCCCCAGCCTCATCTTTCCCAATTTCTTGCTCGACAAGTTGTTGAGAAAATCTATGGCGTATCGGACAGAAAGGGCTGGGTCTCCGTTGGAGCATCTGCGGCAGAAATTGCATGAGGGTTGCGATATGTCAATTAGAGAGCTGGAAGGGCTGTTGTCTCTTCTTGAAGAGAAGAAGAGGAAAATGGAGCTGCAAGAGTCTGAGTCGAGCATGGAGATCATGCTTGGGTTCTTGCATTGCCTTAGAAGGCAAAAGCTAGAGGAGCTCAATGAGATACAAGCTGATCTTGATTACATTAAGGAGGACATGATTGCGGTGGAGAGACGTAGAATTGAACTGTACAGGCAACAACAGAATAGGTCAGTGGGACTGAGAATGCTTGGTGATGATCAGCAGAGCAATGACATTGTCTATAGTGCACAATATGTGCAAGCGCAGATGAGTTCTATCAATTTACATAACAACAGGGCTGTTGTAAATGATGATGTGCAAGACCATACACAGTCTATGCAGCCTGTTACTCCCCAAGTACTGTCGGTGGCAAGGAAGAGGCGAGTACATTCACAGTTCAACGACCTACAAGAGTGTTACTTGCAAAAGCGGCGAAATTGGAACAAACAAAGGGATTCATATGCTATGGATATAGAAGACTATAATCCAGGCCTTGAAGACTTCCACTCTGTGCTAAAGGGCTTTACACAGTACAGCCGATTGAGGGTCATTTCTGAACTGAGACAAGGTGATATTTTTCACTCTGCAAATATTGTGTCGAGCATAGAATTTGATCGAGACGAGCAATTGTTTGCTACTGCTGGGGTTTCACGGTGCATCAAGGTTTTTGAGTTTTCCTCGGTTGTTAATGAACCTGAAGATATCCACTGTGCTGTCGAAGAGATATCCACACGATCCAAACTTAGTTGCTTGAGCTGGAGTAAGTACACTAAAAACCAAATTGCTAGTAGTGACTACGAGGGGATAGTAACAGTTTGGGATGTAACTACTCGTCAGAGTGTGATGGAATATGAGGAGCATGAAAAGCGAGCTTGGAGTGTTGATTTTTCAAGCACCGATCCTTCAATGCTTGTGTCTGGAAGTGATGACTGCAAGGTCAAAATTTGGTGCACAAATCAAGAAGAAAGTGTTCTGAACATCGACATGAAGGCAAACATTTGTTCTGTCAAGTACAATCCCGGATCCAGCTTTTTCGTGGCAGTTGGTTCTGCAGATCATCACATTCATTATTATGACTTGAGAAATGTCAGCCAACCACTGCATGTGTTTAGCGGGCATAGGAAAGCTGTTTCGTATGTGAAATTCTTGTCTAACAATGAACTTTCTTCTGCATCCACTGACAGCACATTGAGACTGTGGGATGTAAAGGAAAACCTGCCGTTGTGCACGTATAGAGGCCACATGAATGAGAAGAACTTTGTAGGTCTTGCAGTAAACAATGACTACATTGCTTGCGGTAGTGAGACAAACGAAGTGTTCGTTTATCACAAGGGTATCCCAAAACCTATGGCTTGGCATAAATTCGGCTCATCGGATTTAAACTACAACAACGAAGAAGACGATGGTGCAGGGTCGTACTTTATCAGTGCTGTATGTTGGAAGAGGGATAGCCCGATGATACTGACGGCGAACAGTCAAGGAACGATTAAAGTTCTTGTACTTGCAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009791 GO:0009812 GO:0009987 GO:0010119 GO:0010228 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40 zf-C3HC4_2

Protein Analysis

641

Amino Acids

72.89

Weight (kDa)

5.7

Isoelectric Point (pI)

56.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_2	PF13923	39 - 77	9.8e-09	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	40 - 77	1.6e-06	Zinc finger, C3HC4 type (RING finger)
Beta-prop_WDR19_1st	PF23389	330 - 470	2.4e-06	WDR19 first beta-propeller
Beta-prop_WDR3_2nd	PF25172	332 - 499	3.7e-08	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	334 - 465	5.3e-12	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	335 - 581	2.4e-33	WDR5 beta-propeller domain
Beta-prop_RIG_2nd	PF23775	335 - 445	3.7e-09	RIG second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	381 - 498	2.9e-07	MABP1/WDR62 first WD40 domain
WD40_Prp19	PF24814	382 - 485	5.3e-10	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	383 - 501	6.9e-16	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	383 - 538	6.7e-19	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	395 - 523	9.5e-13	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML	PF23409	402 - 572	9.5e-08	Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta	PF25391	436 - 566	3.3e-11	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	441 - 550	1.8e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	466 - 572	9.5e-14	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	468 - 575	1.6e-11	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	469 - 545	2.3e-08	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	473 - 582	4.2e-08	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	485 - 638	7.8e-12	THOC3 beta-propeller domain
WD40	PF00400	503 - 539	7.9e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32950 AT2G32950
fragaria_vesca	FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_5g22570 FvH4_5g22570
malus_domestica	MD06G1037700.v1.1 MD10G1328500.v1.1 MD15G1405100.v1.1 MD15G1405200.v1.1 MD16G1272200.v1.1
prunus_persica	Prupe.4G013500_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1
pyrus_communis	pycom10g27830 pycom16g24110
rosa_chinensis	RchiOBHm_Chr5g0001791 RchiOBHm_Chr7g0210341
rosa_laevigata	RLG00000003045 RLG00000030973
rosa_multiflora	Rmu_co8137394.1_g000001 Rmu_sc0003807.1_g000002 Rmu_ssc0000237.1_g000012
rosa_roxburghii	Rroxscaffold_1G00071250 Rroxscaffold_1G00074540 Rroxscaffold_3G00248380
rosa_rugosa	Rorug04G0393100 Rorug04G0393200 Rorug04G0393300 Rorug07G0121200 Rorug07G0121200 Rorug07G0121300
rosa_samantha	Rh5AG014400 Rh5BG017800 Rh5CG015500 Rh5DG015900 Rh7AG253900 Rh7BG248800 Rh7CG270700 Rh7DG261400
rosa_wichuraiana	Rw5G001390 Rw7G021640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1349
Acc36I	ACCTGC	1 cut(s)	1638
AccII	CGCG	1 cut(s)	149
AclWI	GGATC	4 cut(s)	1143, 1307, 1431, 1444
AcoI	YGGCCR	1 cut(s)	166
AcsI	RAATTY	4 cut(s)	1022, 1356, 1556, 1775
AcuI	CTGAAG	1 cut(s)	1131
AdeI	CACNNNGTG	1 cut(s)	925
AfaI	GTAC	8 cut(s)	584, 774, 801, 947, 1181, 1427, 1832, 1914
AfiI	CCNNNNNNNGG	3 cut(s)	28, 230, 958
AflIII	ACRYGT	1 cut(s)	147
AgsI	TTSAA	6 cut(s)	410, 578, 814, 911, 1305, 1320
AhdI	GACNNNNNGTC	1 cut(s)	61
AjnI	CCWGG	1 cut(s)	901
Alw21I	GWGCWC	4 cut(s)	510, 664, 1367, 1643
Alw26I	GTCTC	7 cut(s)	330, 405, 559, 970, 1026, 1600, 1712
Alw44I	GTGCAC	3 cut(s)	660, 1363, 1639
AlwI	GGATC	4 cut(s)	1143, 1307, 1431, 1444
AoxI	GGCC	3 cut(s)	166, 904, 1651
ApaLI	GTGCAC	3 cut(s)	660, 1363, 1639
ApeKI	GCWGC	4 cut(s)	186, 433, 754, 1919
ApoI	RAATTY	4 cut(s)	1022, 1356, 1556, 1775
ArsI	GACNNNNNNTTYG	2 cut(s)	995, 1027
Asp700I	GAANNNNTTC	1 cut(s)	1383
AspLEI	GCGC	1 cut(s)	679
AspS9I	GGNCC	1 cut(s)	11
AsuC2I	CCSGG	1 cut(s)	1434
AsuHPI	GGTGA	4 cut(s)	148, 235, 638, 995
AvaII	GGWCC	1 cut(s)	11
BaeGI	GKGCMC	3 cut(s)	664, 1367, 1643
BaeI	ACNNNNGTAYC	2 cut(s)	1732, 1765
BamHI	GGATCC	1 cut(s)	1436
BanII	GRGCYC	1 cut(s)	510
BbsI	GAAGAC	3 cut(s)	897, 918, 1818
Bbv12I	GWGCWC	4 cut(s)	510, 664, 1367, 1643
BbvI	GCAGC	3 cut(s)	173, 420, 766
BccI	CCATC	2 cut(s)	1252, 1811
BceAI	ACGGC	3 cut(s)	90, 1618, 1896
BcgI	CGANNNNNNTGC	2 cut(s)	42, 76
BciT130I	CCWGG	1 cut(s)	903
BciVI	GTATCC	1 cut(s)	1760
BclI	TGATCA	1 cut(s)	631
BcnI	CCSGG	1 cut(s)	1434
BcoDI	GTCTC	7 cut(s)	330, 405, 559, 970, 1026, 1600, 1712
BfaI	CTAG	2 cut(s)	500, 1200
BfmI	CTRYAG	2 cut(s)	655, 1464
BfuAI	ACCTGC	1 cut(s)	1638
BfuI	GTATCC	1 cut(s)	1760
BisI	GCNGC	9 cut(s)	51, 78, 166, 187, 344, 434, 755, 845, 1920
BlsI	GCNGC	9 cut(s)	52, 79, 167, 188, 345, 435, 756, 846, 1921
BmcAI	AGTACT	1 cut(s)	774
Bme1390I	CCNGG	2 cut(s)	903, 1434
Bme18I	GGWCC	1 cut(s)	11
BmeRI	GACNNNNNGTC	1 cut(s)	61
BmgT120I	GGNCC	1 cut(s)	11
BmiI	GGNNCC	2 cut(s)	96, 1438
BmrFI	CCNGG	2 cut(s)	903, 1434
BmsI	GCATC	3 cut(s)	346, 1080, 1595
BpiI	GAAGAC	3 cut(s)	897, 918, 1818
BplI	GAGNNNNNCTC	2 cut(s)	1076, 1108
BpmI	CTGGAG	2 cut(s)	59, 1192
BpuEI	CTTGAG	2 cut(s)	1186, 1513
BpuMI	CCSGG	1 cut(s)	1434
BsaAI	YACGTR	1 cut(s)	1644
BsaI	GGTCTC	1 cut(s)	330
BsaJI	CCNNGG	2 cut(s)	27, 1092
Bsc4I	CCNNNNNNNGG	3 cut(s)	28, 230, 958
Bse1I	ACTGG	1 cut(s)	42
Bse3DI	GCAATG	3 cut(s)	478, 649, 1701
BseBI	CCWGG	1 cut(s)	903
BseDI	CCNNGG	2 cut(s)	27, 1092
BseGI	GGATG	3 cut(s)	1240, 1586, 1621
BseLI	CCNNNNNNNGG	3 cut(s)	28, 230, 958
BseMI	GCAATG	3 cut(s)	478, 649, 1701
BseMII	CTCAG	3 cut(s)	435, 605, 965
BseNI	ACTGG	1 cut(s)	42
BseRI	GAGGAG	2 cut(s)	518, 1283
BseSI	GKGCMC	3 cut(s)	664, 1367, 1643
BseXI	GCAGC	3 cut(s)	173, 420, 766
BseYI	CCCAGC	3 cut(s)	248, 320, 1055
BsgI	GTGCAG	1 cut(s)	1842
Bsh1236I	CGCG	1 cut(s)	149
Bsh1285I	CGRYCG	1 cut(s)	27
BshFI	GGCC	3 cut(s)	168, 906, 1653
BsiEI	CGRYCG	1 cut(s)	27
BsiHKAI	GWGCWC	4 cut(s)	510, 664, 1367, 1643
BsiSI	CCGG	2 cut(s)	101, 1434
BslFI	GGGAC	2 cut(s)	158, 624
BslI	CCNNNNNNNGG	3 cut(s)	28, 230, 958
BsmAI	GTCTC	7 cut(s)	330, 405, 559, 970, 1026, 1600, 1712
BsmBI	CGTCTC	2 cut(s)	559, 1026
BsmFI	GGGAC	2 cut(s)	158, 624
BsmI	GAATGC	1 cut(s)	624
BsnI	GGCC	3 cut(s)	168, 906, 1653
Bso31I	GGTCTC	1 cut(s)	330
Bsp1286I	GDGCHC	4 cut(s)	510, 664, 1367, 1643
Bsp1407I	TGTACA	1 cut(s)	582
Bsp143I	GATC	8 cut(s)	459, 526, 631, 1027, 1148, 1312, 1436, 1468
BspANI	GGCC	3 cut(s)	168, 906, 1653
BspCNI	CTCAG	3 cut(s)	436, 606, 966
BspFNI	CGCG	1 cut(s)	149
BspLI	GGNNCC	2 cut(s)	96, 1438
BspMAI	CTGCAG	1 cut(s)	1468
BspMI	ACCTGC	1 cut(s)	1638
BspPI	GGATC	4 cut(s)	1143, 1307, 1431, 1444
BspTNI	GGTCTC	1 cut(s)	330
BsrDI	GCAATG	3 cut(s)	478, 649, 1701
BsrGI	TGTACA	1 cut(s)	582
BsrI	ACTGG	1 cut(s)	42
BssECI	CCNNGG	2 cut(s)	27, 1092
BssMI	GATC	8 cut(s)	459, 526, 631, 1027, 1148, 1312, 1436, 1468
Bst2UI	CCWGG	1 cut(s)	903
Bst6I	CTCTTC	6 cut(s)	405, 408, 413, 785, 1128, 1850
BstAPI	GCANNNNNTGC	1 cut(s)	352
BstAUI	TGTACA	1 cut(s)	582
BstBAI	YACGTR	1 cut(s)	1644
BstC8I	GCNNGC	5 cut(s)	163, 675, 1284, 1529, 1708
BstDEI	CTNAG	5 cut(s)	444, 485, 614, 974, 1157
BstDSI	CCRYGG	1 cut(s)	27
BstF5I	GGATG	3 cut(s)	1240, 1586, 1621
BstFNI	CGCG	1 cut(s)	149
BstHHI	GCGC	1 cut(s)	679
BstKTI	GATC	8 cut(s)	462, 529, 634, 1030, 1151, 1315, 1439, 1471
BstMAI	GTCTC	7 cut(s)	330, 405, 559, 970, 1026, 1600, 1712
BstMBI	GATC	8 cut(s)	459, 526, 631, 1027, 1148, 1312, 1436, 1468
BstMCI	CGRYCG	1 cut(s)	27
BstMWI	GCNNNNNNNGC	9 cut(s)	74, 171, 186, 343, 352, 391, 505, 844, 1279
BstNI	CCWGG	1 cut(s)	903
BstNSI	RCATGY	1 cut(s)	1520
BstSCI	CCNGG	2 cut(s)	901, 1432
BstSFI	CTRYAG	2 cut(s)	655, 1464
BstSLI	GKGCMC	3 cut(s)	664, 1367, 1643
BstUI	CGCG	1 cut(s)	149
BstV1I	GCAGC	3 cut(s)	173, 420, 766
BstV2I	GAAGAC	3 cut(s)	897, 918, 1818
BstX2I	RGATCY	1 cut(s)	1436
BstYI	RGATCY	1 cut(s)	1436
BsuI	GTATCC	1 cut(s)	1760
BsuRI	GGCC	3 cut(s)	168, 906, 1653
BtgI	CCRYGG	1 cut(s)	27
BtsCI	GGATG	3 cut(s)	1240, 1586, 1621
BtsI	GCAGTG	2 cut(s)	156, 1511
BtsIMutI	CAGTG	6 cut(s)	156, 612, 1120, 1511, 1590, 1846
BveI	ACCTGC	1 cut(s)	1638
Cac8I	GCNNGC	5 cut(s)	163, 675, 1284, 1529, 1708
CfoI	GCGC	1 cut(s)	679
Cfr13I	GGNCC	1 cut(s)	11
CseI	GACGC	1 cut(s)	155
Csp6I	GTAC	8 cut(s)	583, 773, 800, 946, 1180, 1426, 1831, 1913
CviAII	CATG	8 cut(s)	356, 454, 463, 550, 1274, 1399, 1517, 1657
CviQI	GTAC	8 cut(s)	583, 773, 800, 946, 1180, 1426, 1831, 1913
DdeI	CTNAG	5 cut(s)	444, 485, 614, 974, 1157
DpnI	GATC	8 cut(s)	461, 528, 633, 1029, 1150, 1314, 1438, 1470
DpnII	GATC	8 cut(s)	459, 526, 631, 1027, 1148, 1312, 1436, 1468
DraI	TTTAAA	1 cut(s)	1794
DraIII	CACNNNGTG	1 cut(s)	925
DrdI	GACNNNNNNGTC	1 cut(s)	1349
DriI	GACNNNNNGTC	1 cut(s)	61
DseDI	GACNNNNNNGTC	1 cut(s)	1349
EaeI	YGGCCR	1 cut(s)	166
Eam1104I	CTCTTC	6 cut(s)	405, 408, 413, 785, 1128, 1850
Eam1105I	GACNNNNNGTC	1 cut(s)	61
EarI	CTCTTC	6 cut(s)	405, 408, 413, 785, 1128, 1850
EciI	GGCGGA	2 cut(s)	83, 87
Ecl136II	GAGCTC	1 cut(s)	508
Eco147I	AGGCCT	1 cut(s)	906
Eco24I	GRGCYC	1 cut(s)	510
Eco31I	GGTCTC	1 cut(s)	330
Eco32I	GATATC	2 cut(s)	1117, 1140
Eco47I	GGWCC	1 cut(s)	11
Eco53kI	GAGCTC	1 cut(s)	508
Eco57I	CTGAAG	1 cut(s)	1131
EcoICRI	GAGCTC	1 cut(s)	508
EcoRII	CCWGG	1 cut(s)	901
EcoRV	GATATC	2 cut(s)	1117, 1140
EcoT22I	ATGCAT	1 cut(s)	131
EcoT38I	GRGCYC	1 cut(s)	510
Esp3I	CGTCTC	2 cut(s)	559, 1026
FaeI	CATG	8 cut(s)	359, 457, 466, 553, 1277, 1402, 1520, 1660
FalI	AAGNNNNNCTT	2 cut(s)	513, 545
FaqI	GGGAC	2 cut(s)	158, 624
FatI	CATG	8 cut(s)	355, 453, 462, 549, 1273, 1398, 1516, 1656
FauI	CCCGC	1 cut(s)	1520
FauNDI	CATATG	1 cut(s)	874
FbaI	TGATCA	1 cut(s)	631
Fnu4HI	GCNGC	9 cut(s)	51, 78, 166, 187, 344, 434, 755, 845, 1920
FokI	GGATG	3 cut(s)	1247, 1573, 1628
FriOI	GRGCYC	1 cut(s)	510
Fsp4HI	GCNGC	9 cut(s)	51, 78, 166, 187, 344, 434, 755, 845, 1920
FspBI	CTAG	2 cut(s)	500, 1200
GlaI	GCGC	1 cut(s)	678
GluI	GCNGC	9 cut(s)	51, 78, 166, 187, 344, 434, 755, 845, 1920
GsaI	CCCAGC	3 cut(s)	252, 324, 1059
GsuI	CTGGAG	2 cut(s)	59, 1192
HaeIII	GGCC	3 cut(s)	168, 906, 1653
HapII	CCGG	2 cut(s)	101, 1434
HgaI	GACGC	1 cut(s)	155
HhaI	GCGC	1 cut(s)	679
Hin1II	CATG	8 cut(s)	359, 457, 466, 553, 1277, 1402, 1520, 1660
Hin6I	GCGC	1 cut(s)	677
HinP1I	GCGC	1 cut(s)	677
HinfI	GANTC	5 cut(s)	56, 207, 440, 446, 869
HpaII	CCGG	2 cut(s)	101, 1434
HphI	GGTGA	4 cut(s)	148, 235, 638, 995
Hpy166II	GTNNAC	5 cut(s)	662, 1182, 1365, 1641, 1690
Hpy188I	TCNGA	9 cut(s)	87, 123, 206, 309, 445, 970, 1251, 1389, 1789
Hpy188III	TCNNGA	7 cut(s)	22, 211, 407, 530, 1031, 1332, 1373
Hpy8I	GTNNAC	5 cut(s)	662, 1182, 1365, 1641, 1690
Hpy99I	CGWCG	1 cut(s)	67
HpyAV	CCTTC	5 cut(s)	82, 383, 483, 1326, 1396
HpyCH4IV	ACGT	2 cut(s)	568, 1643
HpyF10VI	GCNNNNNNNGC	9 cut(s)	74, 171, 186, 343, 352, 391, 505, 844, 1279
HpyF3I	CTNAG	5 cut(s)	444, 485, 614, 974, 1157
HpySE526I	ACGT	2 cut(s)	568, 1643
Hsp92II	CATG	8 cut(s)	359, 457, 466, 553, 1277, 1402, 1520, 1660
HspAI	GCGC	1 cut(s)	677
Ksp22I	TGATCA	1 cut(s)	631
Kzo9I	GATC	8 cut(s)	459, 526, 631, 1027, 1148, 1312, 1436, 1468
LmnI	GCTCC	6 cut(s)	40, 100, 334, 430, 505, 1270
Lsp1109I	GCAGC	3 cut(s)	173, 420, 766
LweI	GCATC	3 cut(s)	346, 1080, 1595
MaeI	CTAG	2 cut(s)	500, 1200
MaeII	ACGT	2 cut(s)	568, 1643
MaeIII	GTNAC	6 cut(s)	193, 760, 830, 1205, 1222, 1237
MalI	GATC	8 cut(s)	461, 528, 633, 1029, 1150, 1314, 1438, 1470
MboI	GATC	8 cut(s)	459, 526, 631, 1027, 1148, 1312, 1436, 1468
MfeI	CAATTG	1 cut(s)	1040
MflI	RGATCY	1 cut(s)	1436
MhlI	GDGCHC	4 cut(s)	510, 664, 1367, 1643
MluI	ACGCGT	1 cut(s)	147
MlyI	GAGTC	3 cut(s)	50, 449, 455
MmeI	TCCRAC	2 cut(s)	312, 1832
Mph1103I	ATGCAT	1 cut(s)	131
MroXI	GAANNNNTTC	1 cut(s)	1383
MseI	TTAA	4 cut(s)	540, 1101, 1793, 1902
MslI	CAYNNNNRTG	4 cut(s)	134, 1397, 1485, 1659
MspA1I	CMGCKG	1 cut(s)	186
MspI	CCGG	2 cut(s)	101, 1434
MspR9I	CCNGG	2 cut(s)	903, 1434
MunI	CAATTG	1 cut(s)	1040
Mva1269I	GAATGC	1 cut(s)	624
MvaI	CCWGG	1 cut(s)	903
MvnI	CGCG	1 cut(s)	149
MwoI	GCNNNNNNNGC	9 cut(s)	74, 171, 186, 343, 352, 391, 505, 844, 1279
NciI	CCSGG	1 cut(s)	1434
NdeI	CATATG	1 cut(s)	874
NdeII	GATC	8 cut(s)	459, 526, 631, 1027, 1148, 1312, 1436, 1468
NlaIII	CATG	8 cut(s)	359, 457, 466, 553, 1277, 1402, 1520, 1660
NlaIV	GGNNCC	2 cut(s)	96, 1438
NmuCI	GTSAC	2 cut(s)	193, 1205
NsiI	ATGCAT	1 cut(s)	131
NspI	RCATGY	1 cut(s)	1520
PceI	AGGCCT	1 cut(s)	906
PcsI	WCGNNNNNNNCGW	2 cut(s)	1731, 1812
PctI	GAATGC	1 cut(s)	624
PdmI	GAANNNNTTC	1 cut(s)	1383
PfeI	GAWTC	2 cut(s)	207, 869
PflFI	GACNNNGTC	1 cut(s)	650
PfoI	TCCNGGA	1 cut(s)	1432
PkrI	GCNGC	9 cut(s)	52, 79, 167, 188, 345, 435, 756, 846, 1921
PleI	GAGTC	3 cut(s)	50, 448, 454
PpsI	GAGTC	3 cut(s)	50, 448, 454
Ppu21I	YACGTR	1 cut(s)	1644
Psp124BI	GAGCTC	1 cut(s)	510
Psp6I	CCWGG	1 cut(s)	901
PspFI	CCCAGC	3 cut(s)	248, 320, 1055
PspGI	CCWGG	1 cut(s)	901
PspN4I	GGNNCC	2 cut(s)	96, 1438
PspPI	GGNCC	1 cut(s)	11
PstI	CTGCAG	1 cut(s)	1468
PsuI	RGATCY	1 cut(s)	1436
PsyI	GACNNNGTC	1 cut(s)	650
PvuII	CAGCTG	1 cut(s)	186
RsaI	GTAC	8 cut(s)	584, 774, 801, 947, 1181, 1427, 1832, 1914
RsaNI	GTAC	8 cut(s)	583, 773, 800, 946, 1180, 1426, 1831, 1913
RseI	CAYNNNNRTG	4 cut(s)	134, 1397, 1485, 1659
SacI	GAGCTC	1 cut(s)	510
SaqAI	TTAA	4 cut(s)	540, 1101, 1793, 1902
SatI	GCNGC	9 cut(s)	51, 78, 166, 187, 344, 434, 755, 845, 1920
Sau3AI	GATC	8 cut(s)	459, 526, 631, 1027, 1148, 1312, 1436, 1468
Sau96I	GGNCC	1 cut(s)	11
ScaI	AGTACT	1 cut(s)	774
SchI	GAGTC	3 cut(s)	50, 449, 455
ScrFI	CCNGG	2 cut(s)	903, 1434
SduI	GDGCHC	4 cut(s)	510, 664, 1367, 1643
SfaNI	GCATC	3 cut(s)	346, 1080, 1595
SfcI	CTRYAG	2 cut(s)	655, 1464
SinI	GGWCC	1 cut(s)	11
SmiMI	CAYNNNNRTG	4 cut(s)	134, 1397, 1485, 1659
SmlI	CTYRAG	2 cut(s)	1165, 1492
SmoI	CTYRAG	2 cut(s)	1165, 1492
SseBI	AGGCCT	1 cut(s)	906
SspI	AATATT	1 cut(s)	1006
SspMI	CTAG	2 cut(s)	500, 1200
SstI	GAGCTC	1 cut(s)	510
StuI	AGGCCT	1 cut(s)	906
StyD4I	CCNGG	2 cut(s)	901, 1432
TaiI	ACGT	2 cut(s)	571, 1646
TaqI	TCGA	6 cut(s)	276, 449, 1013, 1030, 1131, 1395
TatI	WGTACW	7 cut(s)	582, 772, 799, 945, 1179, 1425, 1912
TauI	GCSGC	5 cut(s)	53, 80, 168, 346, 847
TfiI	GAWTC	2 cut(s)	207, 869
Tru1I	TTAA	4 cut(s)	540, 1101, 1793, 1902
Tru9I	TTAA	4 cut(s)	540, 1101, 1793, 1902
TscAI	CASTG	6 cut(s)	163, 612, 1127, 1518, 1597, 1846
TseFI	GTSAC	2 cut(s)	193, 1205
TseI	GCWGC	4 cut(s)	186, 433, 754, 1919
Tsp45I	GTSAC	2 cut(s)	193, 1205
TspDTI	ATGAA	7 cut(s)	861, 1119, 1290, 1415, 1469, 1587, 1673
TspGWI	ACGGA	1 cut(s)	318
TspRI	CASTG	6 cut(s)	163, 612, 1127, 1518, 1597, 1846
Tth111I	GACNNNGTC	1 cut(s)	650
VneI	GTGCAC	3 cut(s)	660, 1363, 1639
VpaK11BI	GGWCC	1 cut(s)	11
XapI	RAATTY	4 cut(s)	1022, 1356, 1556, 1775
XceI	RCATGY	1 cut(s)	1520
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1447
XmnI	GAANNNNTTC	1 cut(s)	1383
XspI	CTAG	2 cut(s)	500, 1200
ZrmI	AGTACT	1 cut(s)	774
Zsp2I	ATGCAT	1 cut(s)	131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.