Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001361

Physical Location & Seq

Genomic Coordinates Forward (+)

35411186 .. 35421732

Gene Structure 10547 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1272200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1977 bp

ATGGAGGAGTGCTCGACCGGGGCTCTAGTCCCGGCAGTGAAACCCGAACCAAAAGCGTCAAGCATCACGGATTTGGCAGATCCAGGTAGCGGTGAGGTGGGTCGTCTGGTGCATGAGAAAGAACTGGCAGAGGTAGACAAGGACTTGTTATGCCCAATTTGTATGCAAATGATAAAGGACGCCTTCCTCACAGCGTGTGGTCATAGCTTCTGCTACATGTGCATCATCACCCACCTCCGCAACAAGAGCGACTGCCCTTGCTGCGCCCAGTTCCTCAGCGCCAAACAATTGTTCCCTAATTTTTTGCTGGACAAGCTTCTGAAGAAGACTTCTGCTCGTCAAATTTCCAAAAGTGCATCTCCTGTAGAGCATTTTCGCCAGGCATTGCAGCAGGGATGTGAAGTGTCAATCAAGGAACTAGACACCCTGTTGGCACTCCTGGCAGAGAAGAAGAGGAAAATGGAACAAGAAGAGGCGGAGAGAAATATGCAAATACTGCTTGACTTCTTGAATTGCCTAAGGAAGCAAAAAGTTGACGAGCTTAATGAGGTGCAAACCCATCTCCAATTTATCAAAGAGGACATAGGTGCAGTAGAGAGATGCAGAATGGACTTGTACCGTGCCAGGGACAGATACTCTGTCAAGCTGCGGATGCTTGGAGTGGATGATTCTATTTCTGGGACAAGAAAGCAATGGCATTCCTCTCCAGATAACAACGCCTGTGCGCTTAGTGGACGAGGAGGGATGTCATCTTGGAATCTTCCGAGAAGGGATGCTTTAAGTGCATCAGACTCCCAGTATCTAACTCAAACTGGTCTTGCTGTAGCCAGAAAAAAGCGGGTCCATGCACAGTTTGATGAACTTCAAGAGTGTTACCTGCGAAAGCGGCGTCAGATGGCGAACCAACCGTACTCCCAGCAAGAACGGGACAAAAGTGTAATACAGAGAGAAGGTTATACTGCAGGTCTTGCTGATTTTCAAACAGTGCTCACTACATTAACACAGTACAGTCGAATGAGGGTCATGGCTGAACTTAGGCATGGGGATCTATTTCACTCAGCCAATATAGTATCCAGCATTGAATTTGATTGTGATTACGAGTTATTTGCTACTGCTGGAGTTTCACGGTGCATAAAAGTTTTTGACTTCTCTTCGGTTCTGAATGATCCAGCTGACATGCACTGTCCTGTTGTGGAGATGCCTACACGTTCAAAACTTAGTTGCTTGAGCTGGAACAAGTTTACTAAAAACCATATAGCCAGTACTGACTATGAAGGAATAGTAACAGTTTGGGATGTAAATACTCGGCAGAGTGTCATGGAATATGAAGAGCATGAAAAACGTGCTTGGAGTGTTGATTTTTCATGCATAGACCCCACGATGCTTGTATCTGGTAGTGATGATTGTAAGGTAAAACTTTGGTGCACAAGGCAGGAAGCTAGTGTTATTGATATTGACATGAAAGCCAACATATGTTCCGTCAAGTATAATCCGGGATCTAGCAACTTCATTGCGGTTGGTTCAGCAGACCATCACATCCACTATTATGATTTAAGAAATCCGAGCGAACCACTCCACGTGTTCACTGGGCATGGGAAAACCGTGTCTTATGTAAAATTCTTGTCAAACTATGAGCTTGCCTCTGCATCCACTGATAGCACGCTGCGGTTATGGAATGTGAGGGATAATATTCCAGTTCGTACTTTTAAAGGTCACACAAATGAGAAGAACTTTGTCGGTCTTACAGTAAACAGCGAGTACATTGCGTGTGGCAGCGAAACAAATGAAGTGTTTGTGTATCATAAGGAGATCTCTAAACCTGTGACTTGGCATAAATTTGGGTCGCCTGATTTGGATGACACAGAGGATGATGCGGGGTCGTACTTCATCAGTGCTGTATGTTGGAAGAGTGATAGCCCTACCATGTTAACTGCAAACAGTCAAGGAACCATTAAAGTGTTGGTTCTTGCGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009791 GO:0009812 GO:0009987 GO:0010119 GO:0010228 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40 zf-C3HC4_2

Protein Analysis

659

Amino Acids

74.26

Weight (kDa)

6.23

Isoelectric Point (pI)

46.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	48 - 90	2.8e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	51 - 88	8.2e-06	Ring finger domain
zf-C3HC4	PF00097	51 - 88	1.7e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	51 - 88	3e-11	Zinc finger, C3HC4 type (RING finger)
Beta-prop_WDR5	PF25175	354 - 603	5.4e-32	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	354 - 518	3.2e-13	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	354 - 466	6.2e-08	RIG second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	389 - 518	8.3e-06	MABP1/WDR62 first WD40 domain
Beta-prop_THOC3	PF25174	403 - 520	3.4e-16	THOC3 beta-propeller domain
WD40_Prp19	PF24814	405 - 481	9.9e-07	Prp19 WD40 domain
WD40_Gbeta	PF25391	406 - 523	8e-07	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	406 - 501	9.1e-11	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	415 - 542	5e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	417 - 579	4e-08	MABP1/WDR62 second WD40 domain
Beta-prop_EML	PF23409	422 - 604	2.5e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	441 - 590	2.4e-10	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	462 - 580	2e-11	WDHD1 first WD40 domain
Beta-prop_EIPR1	PF23609	487 - 558	8.8e-06	EIPR1 beta-propeller
WD40_Prp19	PF24814	488 - 610	8.5e-13	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	489 - 600	1.8e-12	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	501 - 573	2.8e-07	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	527 - 655	1.1e-11	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32950 AT2G32950
fragaria_vesca	FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_5g22570 FvH4_5g22570
malus_domestica	MD06G1037700.v1.1 MD10G1328500.v1.1 MD15G1405100.v1.1 MD15G1405200.v1.1 MD16G1272200.v1.1
prunus_persica	Prupe.4G013500_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1
pyrus_communis	pycom10g27830 pycom16g24110
rosa_chinensis	RchiOBHm_Chr5g0001791 RchiOBHm_Chr7g0210341
rosa_laevigata	RLG00000003045 RLG00000030973
rosa_multiflora	Rmu_co8137394.1_g000001 Rmu_sc0003807.1_g000002 Rmu_ssc0000237.1_g000012
rosa_roxburghii	Rroxscaffold_1G00071250 Rroxscaffold_1G00074540 Rroxscaffold_3G00248380
rosa_rugosa	Rorug04G0393100 Rorug04G0393200 Rorug04G0393300 Rorug07G0121200 Rorug07G0121200 Rorug07G0121300
rosa_samantha	Rh5AG014400 Rh5BG017800 Rh5CG015500 Rh5DG015900 Rh7AG253900 Rh7BG248800 Rh7CG270700 Rh7DG261400
rosa_wichuraiana	Rw5G001390 Rw7G021640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	885, 953
AccI	GTMKAC	1 cut(s)	135
AclWI	GGATC	4 cut(s)	74, 1053, 1160, 1504
AcsI	RAATTY	4 cut(s)	342, 1082, 1616, 1835
AcuI	CTGAAG	1 cut(s)	341
AcvI	CACGTG	1 cut(s)	1579
AcyI	GRCGYC	2 cut(s)	180, 889
AdeI	CACNNNGTG	1 cut(s)	195
AfaI	GTAC	7 cut(s)	617, 911, 1007, 1264, 1702, 1760, 1883
AfiI	CCNNNNNNNGG	2 cut(s)	89, 625
AflIII	ACRYGT	3 cut(s)	216, 1205, 1578
AgsI	TTSAA	5 cut(s)	511, 866, 980, 1082, 1212
AjnI	CCWGG	4 cut(s)	82, 378, 438, 623
AjuI	GAANNNNNNNTTGG	2 cut(s)	275, 307
AluBI	AGCT	8 cut(s)	207, 316, 541, 646, 1172, 1230, 1439, 1636
AluI	AGCT	8 cut(s)	207, 316, 541, 646, 1172, 1230, 1439, 1636
Alw21I	GWGCWC	3 cut(s)	14, 990, 1427
Alw44I	GTGCAC	1 cut(s)	1423
AlwI	GGATC	4 cut(s)	74, 1053, 1160, 1504
ApaLI	GTGCAC	1 cut(s)	1423
ApeKI	GCWGC	5 cut(s)	261, 388, 646, 1663, 1773
ApoI	RAATTY	4 cut(s)	342, 1082, 1616, 1835
ArsI	GACNNNNNNTTYG	2 cut(s)	874, 906
AspLEI	GCGC	3 cut(s)	266, 281, 727
AspS9I	GGNCC	1 cut(s)	841
AsuC2I	CCSGG	3 cut(s)	19, 32, 1494
AsuHPI	GGTGA	2 cut(s)	104, 220
AvaII	GGWCC	1 cut(s)	841
AxyI	CCTNAGG	1 cut(s)	518
BaeGI	GKGCMC	1 cut(s)	1427
BanII	GRGCYC	1 cut(s)	25
BbrPI	CACGTG	1 cut(s)	1579
BbsI	GAAGAC	1 cut(s)	332
Bbv12I	GWGCWC	3 cut(s)	14, 990, 1427
BbvCI	CCTCAGC	1 cut(s)	275
BbvI	GCAGC	5 cut(s)	248, 400, 633, 1650, 1785
BccI	CCATC	3 cut(s)	567, 889, 1539
BcgI	CGANNNNNNTGC	4 cut(s)	1088, 1122, 1745, 1779
BciT130I	CCWGG	4 cut(s)	84, 380, 440, 625
BciVI	GTATCC	1 cut(s)	1081
BcnI	CCSGG	3 cut(s)	19, 32, 1494
BfaI	CTAG	4 cut(s)	26, 419, 1440, 1500
BfmI	CTRYAG	3 cut(s)	363, 822, 960
BfoI	RGCGCY	1 cut(s)	282
BfuAI	ACCTGC	2 cut(s)	885, 953
BfuI	GTATCC	1 cut(s)	1081
BglII	AGATCT	1 cut(s)	1809
BisI	GCNGC	7 cut(s)	262, 389, 647, 887, 1664, 1774, 1971
BlsI	GCNGC	7 cut(s)	263, 390, 648, 888, 1665, 1775, 1972
BmcAI	AGTACT	1 cut(s)	1264
Bme1390I	CCNGG	7 cut(s)	19, 32, 84, 380, 440, 625, 1494
Bme18I	GGWCC	1 cut(s)	841
BmgT120I	GGNCC	1 cut(s)	841
BmiI	GGNNCC	2 cut(s)	842, 1948
BmrFI	CCNGG	7 cut(s)	19, 32, 84, 380, 440, 625, 1494
BmrI	ACTGGG	3 cut(s)	262, 790, 1596
BmuI	ACTGGG	3 cut(s)	262, 790, 1596
BpiI	GAAGAC	1 cut(s)	332
BplI	GAGNNNNNCTC	3 cut(s)	28, 1625, 1657
BpmI	CTGGAG	2 cut(s)	690, 1137
Bpu10I	CCTNAGC	1 cut(s)	275
BpuEI	CTTGAG	1 cut(s)	1246
BpuMI	CCSGG	3 cut(s)	19, 32, 1494
BsaAI	YACGTR	1 cut(s)	1579
BsaHI	GRCGYC	2 cut(s)	180, 889
BsaJI	CCNNGG	2 cut(s)	18, 624
Bsc4I	CCNNNNNNNGG	2 cut(s)	89, 625
Bse1I	ACTGG	7 cut(s)	129, 268, 796, 817, 1260, 1591, 1694
Bse21I	CCTNAGG	1 cut(s)	518
Bse3DI	GCAATG	4 cut(s)	383, 698, 1509, 1761
BseBI	CCWGG	4 cut(s)	84, 380, 440, 625
BseDI	CCNNGG	2 cut(s)	18, 624
BseLI	CCNNNNNNNGG	2 cut(s)	89, 625
BseMI	GCAATG	4 cut(s)	383, 698, 1509, 1761
BseMII	CTCAG	2 cut(s)	289, 1071
BseNI	ACTGG	7 cut(s)	129, 268, 796, 817, 1260, 1591, 1694
BseRI	GAGGAG	2 cut(s)	20, 753
BseSI	GKGCMC	1 cut(s)	1427
BseXI	GCAGC	5 cut(s)	248, 400, 633, 1650, 1785
BseYI	CCCAGC	1 cut(s)	915
BsgI	GTGCAG	1 cut(s)	609
Bsh1285I	CGRYCG	1 cut(s)	18
BsiEI	CGRYCG	1 cut(s)	18
BsiHKAI	GWGCWC	3 cut(s)	14, 990, 1427
BsiSI	CCGG	3 cut(s)	18, 32, 1493
BslFI	GGGAC	4 cut(s)	14, 641, 694, 941
BslI	CCNNNNNNNGG	2 cut(s)	89, 625
BsmFI	GGGAC	4 cut(s)	14, 641, 694, 941
BsmI	GAATGC	1 cut(s)	697
Bsp1286I	GDGCHC	4 cut(s)	14, 25, 990, 1427
Bsp143I	GATC	5 cut(s)	79, 1045, 1165, 1496, 1809
BspCNI	CTCAG	2 cut(s)	288, 1070
BspLI	GGNNCC	2 cut(s)	842, 1948
BspMAI	CTGCAG	1 cut(s)	964
BspMI	ACCTGC	2 cut(s)	885, 953
BspPI	GGATC	4 cut(s)	74, 1053, 1160, 1504
BspQI	GCTCTTC	1 cut(s)	1323
BsrDI	GCAATG	4 cut(s)	383, 698, 1509, 1761
BsrI	ACTGG	7 cut(s)	129, 268, 796, 817, 1260, 1591, 1694
BssECI	CCNNGG	2 cut(s)	18, 624
BssMI	GATC	5 cut(s)	79, 1045, 1165, 1496, 1809
BssNI	GRCGYC	2 cut(s)	180, 889
Bst2UI	CCWGG	4 cut(s)	84, 380, 440, 625
Bst6I	CTCTTC	5 cut(s)	446, 465, 1156, 1323, 1901
BstACI	GRCGYC	2 cut(s)	180, 889
BstAPI	GCANNNNNTGC	2 cut(s)	496, 968
BstBAI	YACGTR	1 cut(s)	1579
BstC8I	GCNNGC	2 cut(s)	1638, 1661
BstDEI	CTNAG	6 cut(s)	275, 518, 728, 1034, 1057, 1217
BstH2I	RGCGCY	1 cut(s)	282
BstHHI	GCGC	3 cut(s)	266, 281, 727
BstKTI	GATC	5 cut(s)	82, 1048, 1168, 1499, 1812
BstMBI	GATC	5 cut(s)	79, 1045, 1165, 1496, 1809
BstMCI	CGRYCG	1 cut(s)	18
BstNI	CCWGG	4 cut(s)	84, 380, 440, 625
BstNSI	RCATGY	2 cut(s)	220, 1180
BstSCI	CCNGG	7 cut(s)	17, 30, 82, 378, 438, 623, 1492
BstSFI	CTRYAG	3 cut(s)	363, 822, 960
BstSLI	GKGCMC	1 cut(s)	1427
BstV1I	GCAGC	5 cut(s)	248, 400, 633, 1650, 1785
BstV2I	GAAGAC	1 cut(s)	332
BstX2I	RGATCY	4 cut(s)	79, 1045, 1496, 1809
BstYI	RGATCY	4 cut(s)	79, 1045, 1496, 1809
Bsu36I	CCTNAGG	1 cut(s)	518
BsuI	GTATCC	1 cut(s)	1081
BtsI	GCAGTG	1 cut(s)	42
BtsIMutI	CAGTG	6 cut(s)	42, 990, 1180, 1584, 1650, 1897
BveI	ACCTGC	2 cut(s)	885, 953
Cac8I	GCNNGC	2 cut(s)	1638, 1661
CfoI	GCGC	3 cut(s)	266, 281, 727
Cfr13I	GGNCC	1 cut(s)	841
CseI	GACGC	3 cut(s)	45, 188, 878
Csp6I	GTAC	7 cut(s)	616, 910, 1006, 1263, 1701, 1759, 1882
CspCI	CAANNNNNGTGG	2 cut(s)	1366, 1401
CviQI	GTAC	7 cut(s)	616, 910, 1006, 1263, 1701, 1759, 1882
DdeI	CTNAG	6 cut(s)	275, 518, 728, 1034, 1057, 1217
DpnI	GATC	5 cut(s)	81, 1047, 1167, 1498, 1811
DpnII	GATC	5 cut(s)	79, 1045, 1165, 1496, 1809
DraI	TTTAAA	1 cut(s)	1708
DraIII	CACNNNGTG	1 cut(s)	195
Eam1104I	CTCTTC	5 cut(s)	446, 465, 1156, 1323, 1901
EarI	CTCTTC	5 cut(s)	446, 465, 1156, 1323, 1901
EciI	GGCGGA	1 cut(s)	491
Eco24I	GRGCYC	1 cut(s)	25
Eco47I	GGWCC	1 cut(s)	841
Eco57I	CTGAAG	1 cut(s)	341
Eco72I	CACGTG	1 cut(s)	1579
Eco81I	CCTNAGG	1 cut(s)	518
EcoRII	CCWGG	4 cut(s)	82, 378, 438, 623
EcoT22I	ATGCAT	1 cut(s)	1370
EcoT38I	GRGCYC	1 cut(s)	25
FalI	AAGNNNNNCTT	4 cut(s)	167, 199, 760, 792
FaqI	GGGAC	4 cut(s)	14, 641, 694, 941
FauI	CCCGC	2 cut(s)	831, 1867
FauNDI	CATATG	1 cut(s)	1472
FblI	GTMKAC	1 cut(s)	135
Fnu4HI	GCNGC	7 cut(s)	262, 389, 647, 887, 1664, 1774, 1971
FriOI	GRGCYC	1 cut(s)	25
Fsp4HI	GCNGC	7 cut(s)	262, 389, 647, 887, 1664, 1774, 1971
FspBI	CTAG	4 cut(s)	26, 419, 1440, 1500
GlaI	GCGC	3 cut(s)	265, 280, 726
GluI	GCNGC	7 cut(s)	262, 389, 647, 887, 1664, 1774, 1971
GsaI	CCCAGC	1 cut(s)	919
GsuI	CTGGAG	2 cut(s)	690, 1137
HaeII	RGCGCY	1 cut(s)	282
HapII	CCGG	3 cut(s)	18, 32, 1493
HgaI	GACGC	3 cut(s)	45, 188, 878
HhaI	GCGC	3 cut(s)	266, 281, 727
Hin1I	GRCGYC	2 cut(s)	180, 889
Hin6I	GCGC	3 cut(s)	264, 279, 725
HinP1I	GCGC	3 cut(s)	264, 279, 725
HincII	GTYRAC	2 cut(s)	535, 1929
HindII	GTYRAC	2 cut(s)	535, 1929
HindIII	AAGCTT	1 cut(s)	314
HinfI	GANTC	3 cut(s)	668, 757, 791
HpaI	GTTAAC	1 cut(s)	1929
HpaII	CCGG	3 cut(s)	18, 32, 1493
HphI	GGTGA	2 cut(s)	104, 220
Hpy166II	GTNNAC	8 cut(s)	136, 535, 734, 1242, 1425, 1584, 1750, 1929
Hpy188I	TCNGA	6 cut(s)	321, 765, 790, 894, 1161, 1563
Hpy188III	TCNNGA	3 cut(s)	508, 707, 866
Hpy8I	GTNNAC	8 cut(s)	136, 535, 734, 1242, 1425, 1584, 1750, 1929
HpyAV	CCTTC	4 cut(s)	193, 762, 944, 1268
HpyCH4IV	ACGT	3 cut(s)	1207, 1342, 1578
HpyF3I	CTNAG	6 cut(s)	275, 518, 728, 1034, 1057, 1217
HpySE526I	ACGT	3 cut(s)	1207, 1342, 1578
Hsp92I	GRCGYC	2 cut(s)	180, 889
HspAI	GCGC	3 cut(s)	264, 279, 725
KspAI	GTTAAC	1 cut(s)	1929
Kzo9I	GATC	5 cut(s)	79, 1045, 1165, 1496, 1809
LguI	GCTCTTC	1 cut(s)	1323
Lsp1109I	GCAGC	5 cut(s)	248, 400, 633, 1650, 1785
MaeI	CTAG	4 cut(s)	26, 419, 1440, 1500
MaeII	ACGT	3 cut(s)	1207, 1342, 1578
MaeIII	GTNAC	4 cut(s)	872, 1282, 1712, 1822
MalI	GATC	5 cut(s)	81, 1047, 1167, 1498, 1811
MboI	GATC	5 cut(s)	79, 1045, 1165, 1496, 1809
MfeI	CAATTG	1 cut(s)	287
MflI	RGATCY	4 cut(s)	79, 1045, 1496, 1809
MhlI	GDGCHC	4 cut(s)	14, 25, 990, 1427
MluCI	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 1082, 1616, 1835
MlyI	GAGTC	1 cut(s)	785
MmeI	TCCRAC	1 cut(s)	1883
Mph1103I	ATGCAT	1 cut(s)	1370
MseI	TTAA	7 cut(s)	543, 779, 998, 1553, 1707, 1928, 1953
MslI	CAYNNNNRTG	3 cut(s)	1545, 1719, 1955
MspA1I	CMGCKG	1 cut(s)	1172
MspI	CCGG	3 cut(s)	18, 32, 1493
MspR9I	CCNGG	7 cut(s)	19, 32, 84, 380, 440, 625, 1494
MunI	CAATTG	1 cut(s)	287
Mva1269I	GAATGC	1 cut(s)	697
MvaI	CCWGG	4 cut(s)	84, 380, 440, 625
NciI	CCSGG	3 cut(s)	19, 32, 1494
NdeI	CATATG	1 cut(s)	1472
NdeII	GATC	5 cut(s)	79, 1045, 1165, 1496, 1809
NlaIV	GGNNCC	2 cut(s)	842, 1948
NmeAIII	GCCGAG	1 cut(s)	1285
NmuCI	GTSAC	2 cut(s)	1712, 1822
NsiI	ATGCAT	1 cut(s)	1370
NspI	RCATGY	2 cut(s)	220, 1180
PciI	ACATGT	1 cut(s)	216
PciSI	GCTCTTC	1 cut(s)	1323
PctI	GAATGC	1 cut(s)	697
PfeI	GAWTC	2 cut(s)	668, 757
PfoI	TCCNGGA	1 cut(s)	1492
PkrI	GCNGC	7 cut(s)	263, 390, 648, 888, 1665, 1775, 1972
PleI	GAGTC	1 cut(s)	785
PmaCI	CACGTG	1 cut(s)	1579
PmlI	CACGTG	1 cut(s)	1579
PpsI	GAGTC	1 cut(s)	785
Ppu21I	YACGTR	1 cut(s)	1579
PscI	ACATGT	1 cut(s)	216
Psp6I	CCWGG	4 cut(s)	82, 378, 438, 623
PspCI	CACGTG	1 cut(s)	1579
PspFI	CCCAGC	1 cut(s)	915
PspGI	CCWGG	4 cut(s)	82, 378, 438, 623
PspN4I	GGNNCC	2 cut(s)	842, 1948
PspPI	GGNCC	1 cut(s)	841
PsrI	GAACNNNNNNTAC	2 cut(s)	893, 925
PstI	CTGCAG	1 cut(s)	964
PsuI	RGATCY	4 cut(s)	79, 1045, 1496, 1809
PvuII	CAGCTG	1 cut(s)	1172
RsaI	GTAC	7 cut(s)	617, 911, 1007, 1264, 1702, 1760, 1883
RsaNI	GTAC	7 cut(s)	616, 910, 1006, 1263, 1701, 1759, 1882
RseI	CAYNNNNRTG	3 cut(s)	1545, 1719, 1955
SapI	GCTCTTC	1 cut(s)	1323
SaqAI	TTAA	7 cut(s)	543, 779, 998, 1553, 1707, 1928, 1953
SatI	GCNGC	7 cut(s)	262, 389, 647, 887, 1664, 1774, 1971
Sau3AI	GATC	5 cut(s)	79, 1045, 1165, 1496, 1809
Sau96I	GGNCC	1 cut(s)	841
ScaI	AGTACT	1 cut(s)	1264
SchI	GAGTC	1 cut(s)	785
ScrFI	CCNGG	7 cut(s)	19, 32, 84, 380, 440, 625, 1494
SduI	GDGCHC	4 cut(s)	14, 25, 990, 1427
SfcI	CTRYAG	3 cut(s)	363, 822, 960
SinI	GGWCC	1 cut(s)	841
SmiMI	CAYNNNNRTG	3 cut(s)	1545, 1719, 1955
SmlI	CTYRAG	1 cut(s)	1225
SmoI	CTYRAG	1 cut(s)	1225
Sse9I	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 1082, 1616, 1835
SspI	AATATT	1 cut(s)	1690
SspMI	CTAG	4 cut(s)	26, 419, 1440, 1500
StyD4I	CCNGG	7 cut(s)	17, 30, 82, 378, 438, 623, 1492
TaiI	ACGT	3 cut(s)	1210, 1345, 1581
TaqI	TCGA	2 cut(s)	14, 1012
TaqII	GACCGA	1 cut(s)	1727
TasI	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 1082, 1616, 1835
TatI	WGTACW	3 cut(s)	1005, 1262, 1758
TauI	GCSGC	2 cut(s)	889, 1973
TfiI	GAWTC	2 cut(s)	668, 757
Tru1I	TTAA	7 cut(s)	543, 779, 998, 1553, 1707, 1928, 1953
Tru9I	TTAA	7 cut(s)	543, 779, 998, 1553, 1707, 1928, 1953
TscAI	CASTG	6 cut(s)	42, 990, 1187, 1591, 1657, 1897
TseFI	GTSAC	2 cut(s)	1712, 1822
TseI	GCWGC	5 cut(s)	261, 388, 646, 1663, 1773
Tsp45I	GTSAC	2 cut(s)	1712, 1822
TspDTI	ATGAA	9 cut(s)	873, 1287, 1341, 1350, 1353, 1475, 1498, 1800, 1876
TspGWI	ACGGA	2 cut(s)	83, 1468
TspRI	CASTG	6 cut(s)	42, 990, 1187, 1591, 1657, 1897
VneI	GTGCAC	1 cut(s)	1423
VpaK11BI	GGWCC	1 cut(s)	841
XapI	RAATTY	4 cut(s)	342, 1082, 1616, 1835
XceI	RCATGY	2 cut(s)	220, 1180
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1583, 1957
XmiI	GTMKAC	1 cut(s)	135
XspI	CTAG	4 cut(s)	26, 419, 1440, 1500
ZrmI	AGTACT	1 cut(s)	1264
Zsp2I	ATGCAT	1 cut(s)	1370

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.