Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001361

Physical Location & Seq

Genomic Coordinates Reverse (-)

40767618 .. 40772483

Gene Structure 4866 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1328500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGGGAGAGAGCTCAATGGGTGGTGCGCTGGTCCCCACCGTGAAATCCGAATACTTCCAGGACTCCGCCGCCGAAACGGCGCCGTTTGACGACGAGCCGGACAAGGACATGAAGTGTCCGATATGCATGCAGATCATCAGAGACGCCTGCCTCACCGCCTGCGGCCATAGTTTCTGCCACGTCTGCATTTCAACTCACCTCCGCATCAAGAGCGATTGCCCTTGCTGCGCTAGCTCCCTCACCCCCGCCAGCATTTTCCCCAATTTCTTGCTCGACAAGTTGCTGAAAAATGTTTTGGATTCTCGGATGGCGAAGAATTTTGAACTTCTTAGTCGGAAATTGAACAAGGGCTGCGAGATTTCGATCAAAGAGCTGGACGGCCTTTTGTCCTTGCTGGAAGAGAAGAGGAGAAAAATGGAGCTACAAGAAGCCGAGAACAGCATGGACATCATGCTTAGCTTCTTACATTGTCTGAGAAGGCAAAAGCTTCAAGAGCTCAATGAGTTAGAGGCCGATCTCAGGTACATCAAGGAGGACATTACTGCCGTTGAGAGGCATAGACTAGAACTATGCAGCTGGGAACAGGAGAGGTCCGCAAAGCTGAGAATGCTTGTTCCCGGTGATCAGCATGGCAACGGCATTGCCTGCAGCACTCAATATGTGCAAGACCGGATGAGTTCTTTCAACCTACAGAACAAGAGAGCTGATGTAAACGGGCAATCAAGCTCCAAGCTGCTCCAACTGAAGGATGCTTATGGAAGGTCTGAAATGCAGTGTGTTACAACACGAGGAGTACTATCAGTAGCAAGAAAAAGGCGTGTCCATTCGCAGTTTAATGATCTACAAGACTGCTACTTGCAAAAGCGACGAAATTGGAACAGACAGGAAGAGGACACAAACGCCATGGACATAGAAGGTTACAATCCAGGCCTTGAAGATTTTCAATCTGTGCTTGCAAGCTTTACACAATACAGTCGATTGAGAGTCGTTGCTGAACTGAGTCACGGTGATCTTTTTCACTCTGCTAATATTGTGTCAAGTATAGAATTTGATCGTGATGAAGAGCTCTTTGCTACTGCTGGAGTTTCACGGTGCATCAAGGTTTTTGAATTTTCCTCGGTGGTTAACGAACCAACCGATATCCACTGTCCTATTGTGGAGATATCCTCACGATCCAAACTTAGTTGCTTGAGCTGGAATAAGTACACTAGAAACCATATTGCTAGTAGTGACTACGAGGGGGTAGTAACTGTTTGGGATGTAAATACTTGTCAGAGTATGATGGAATATGAGGAGCATGAAAAGCGAGCTTGGAGCGTTGATTTTTCATGCACCGATCCTTCAATGCTTGTGTCCGGAAGTGATGACTGCAAGGTCAAAATTTGGTGCACAAAGCAAGAACCAAGTGTTCTTAACATCGACATGAAGGCAAATATATGTTCTGTCAAGTATAATCCTGGATCCAGCTTTTTCGTGGCAGTTGGCTCTGCTGACCATCACATTCATTATTATGACTTGAGAAACATCAGCCAACCACTGCACGTGTTCCGTGGGCATAGAAAAGCTGTTTCGTATGTAAAATTCTTGTCTAACAACGAACTTGCTTCTGCATCCACTGACAGCACACTGCGGTTGTGGGACGTAAAGGAAAACTTGCCCTTGTGCATGTACAGAGGCCACATGAACGAGAAGAACTTTGTAGGTCTTGCAGTAAATAATGAATACATTGCTTGTGGCAGCGAGACAAATGAACTGTTCGTATACCATAAGGCCATCTCGAAACCTATGACTTGGCACAGATTTGGCTCTTCGGATTTAGGCGATAATGACGACGATCCAGGACCACACTTTATCAGTGCGGTATGTTGGAAGAGGGATAGCCCTATGATATTGACTGCAAACAGTCAAGGAACGATTAAAGTGCTCGTACTTGCAGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009791 GO:0009812 GO:0009987 GO:0010119 GO:0010228 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40 zf-C3HC4_2

Protein Analysis

647

Amino Acids

73.25

Weight (kDa)

5.93

Isoelectric Point (pI)

55.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_2	PF13923	38 - 76	2.1e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	39 - 76	2.1e-06	Zinc finger, C3HC4 type (RING finger)
Beta-prop_WDR3_1st	PF25173	342 - 505	3.6e-13	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	343 - 589	1.3e-31	WDR5 beta-propeller domain
Beta-prop_RIG_2nd	PF23775	343 - 454	8.2e-08	RIG second beta-propeller
Beta-prop_WDR3_2nd	PF25172	345 - 462	1e-05	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	388 - 506	6.4e-06	MABP1/WDR62 first WD40 domain
Beta-prop_THOC3	PF25174	391 - 509	2.4e-15	THOC3 beta-propeller domain
WD40_Prp19	PF24814	391 - 506	3.1e-11	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	392 - 546	2.3e-18	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	404 - 531	4.4e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	404 - 565	2.5e-08	MABP1/WDR62 second WD40 domain
Beta-prop_EML	PF23409	410 - 584	1.9e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	413 - 558	2.2e-11	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	438 - 595	1.2e-12	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	444 - 574	1.8e-10	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	446 - 557	4.6e-09	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	474 - 580	6.6e-14	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	476 - 588	1.5e-11	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	476 - 551	1.9e-09	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	479 - 596	2e-08	CDC20/Fizzy WD40 domain
WD40	PF00400	511 - 547	1.6e-06	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	514 - 643	3.3e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32950 AT2G32950
fragaria_vesca	FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_5g22570 FvH4_5g22570
malus_domestica	MD06G1037700.v1.1 MD10G1328500.v1.1 MD15G1405100.v1.1 MD15G1405200.v1.1 MD16G1272200.v1.1
prunus_persica	Prupe.4G013500_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1
pyrus_communis	pycom10g27830 pycom16g24110
rosa_chinensis	RchiOBHm_Chr5g0001791 RchiOBHm_Chr7g0210341
rosa_laevigata	RLG00000003045 RLG00000030973
rosa_multiflora	Rmu_co8137394.1_g000001 Rmu_sc0003807.1_g000002 Rmu_ssc0000237.1_g000012
rosa_roxburghii	Rroxscaffold_1G00071250 Rroxscaffold_1G00074540 Rroxscaffold_3G00248380
rosa_rugosa	Rorug04G0393100 Rorug04G0393200 Rorug04G0393300 Rorug07G0121200 Rorug07G0121200 Rorug07G0121300
rosa_samantha	Rh5AG014400 Rh5BG017800 Rh5CG015500 Rh5DG015900 Rh7AG253900 Rh7BG248800 Rh7CG270700 Rh7DG261400
rosa_wichuraiana	Rw5G001390 Rw7G021640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1373
AccB1I	GGYRCC	1 cut(s)	79
AccI	GTMKAC	1 cut(s)	1761
AccIII	TCCGGA	1 cut(s)	1355
AciI	CCGC	9 cut(s)	66, 69, 156, 162, 202, 246, 594, 1630, 1859
AclWI	GGATC	5 cut(s)	1167, 1331, 1455, 1468, 1829
AcoI	YGGCCR	1 cut(s)	163
AcsI	RAATTY	5 cut(s)	316, 1046, 1109, 1380, 1580
AcuI	CTGAAG	1 cut(s)	764
AcvI	CACGTG	1 cut(s)	1543
AcyI	GRCGYC	2 cut(s)	80, 144
AfaI	GTAC	5 cut(s)	524, 795, 1205, 1670, 1929
AfiI	CCNNNNNNNGG	3 cut(s)	519, 552, 745
AflIII	ACRYGT	1 cut(s)	1542
AgsI	TTSAA	9 cut(s)	192, 323, 343, 491, 685, 935, 944, 1109, 1344
AjiI	CACGTC	1 cut(s)	181
AjnI	CCWGG	4 cut(s)	57, 925, 1456, 1837
AjuI	GAANNNNNNNTTGG	2 cut(s)	278, 310
Alw21I	GWGCWC	5 cut(s)	14, 498, 1068, 1391, 1926
Alw26I	GTCTC	2 cut(s)	135, 1736
Alw44I	GTGCAC	1 cut(s)	1387
AlwI	GGATC	5 cut(s)	1167, 1331, 1455, 1468, 1829
Aor13HI	TCCGGA	1 cut(s)	1355
AoxI	GGCC	6 cut(s)	163, 379, 510, 928, 1675, 1770
ApaLI	GTGCAC	1 cut(s)	1387
ApeKI	GCWGC	7 cut(s)	225, 351, 573, 648, 733, 1737, 1934
ApoI	RAATTY	5 cut(s)	316, 1046, 1109, 1380, 1580
Asp700I	GAANNNNTTC	1 cut(s)	939
AspLEI	GCGC	3 cut(s)	28, 82, 230
AspS9I	GGNCC	3 cut(s)	31, 591, 1841
AsuC2I	CCSGG	1 cut(s)	618
AsuHPI	GGTGA	5 cut(s)	145, 188, 232, 632, 1019
AsuNHI	GCTAGC	1 cut(s)	230
AvaII	GGWCC	3 cut(s)	31, 591, 1841
BaeGI	GKGCMC	1 cut(s)	1391
BamHI	GGATCC	1 cut(s)	1460
BanI	GGYRCC	1 cut(s)	79
BanII	GRGCYC	3 cut(s)	14, 498, 1068
BauI	CACGAG	1 cut(s)	786
BbrPI	CACGTG	1 cut(s)	1543
Bbv12I	GWGCWC	5 cut(s)	14, 498, 1068, 1391, 1926
BbvI	GCAGC	6 cut(s)	212, 338, 585, 660, 720, 1749
BccI	CCATC	4 cut(s)	301, 1276, 1503, 1781
BceAI	ACGGC	5 cut(s)	67, 93, 394, 530, 652
BcgI	CGANNNNNNTGC	4 cut(s)	109, 143, 1903, 1937
BciT130I	CCWGG	4 cut(s)	59, 927, 1458, 1839
BclI	TGATCA	1 cut(s)	622
BcnI	CCSGG	1 cut(s)	618
BcoDI	GTCTC	2 cut(s)	135, 1736
BfaI	CTAG	4 cut(s)	231, 563, 1209, 1224
BfmI	CTRYAG	2 cut(s)	646, 689
BfoI	RGCGCY	1 cut(s)	83
BglI	GCCNNNNNGGC	1 cut(s)	77
BisI	GCNGC	9 cut(s)	69, 163, 226, 352, 574, 649, 734, 1738, 1935
BlpI	GCTNAGC	1 cut(s)	455
BlsI	GCNGC	9 cut(s)	70, 164, 227, 353, 575, 650, 735, 1739, 1936
BmcAI	AGTACT	1 cut(s)	795
Bme1390I	CCNGG	5 cut(s)	59, 618, 927, 1458, 1839
Bme18I	GGWCC	3 cut(s)	31, 591, 1841
BmgBI	CACGTC	1 cut(s)	181
BmgT120I	GGNCC	3 cut(s)	31, 591, 1841
BmiI	GGNNCC	3 cut(s)	33, 81, 1462
BmrFI	CCNGG	5 cut(s)	59, 618, 927, 1458, 1839
BmsI	GCATC	4 cut(s)	213, 739, 1104, 1619
BmtI	GCTAGC	1 cut(s)	234
BplI	GAGNNNNNCTC	2 cut(s)	1151, 1183
BpmI	CTGGAG	1 cut(s)	1101
Bpu1102I	GCTNAGC	1 cut(s)	455
BpuEI	CTTGAG	2 cut(s)	1210, 1537
BpuMI	CCSGG	1 cut(s)	618
BsaAI	YACGTR	1 cut(s)	1543
BsaBI	GATNNNNATC	1 cut(s)	362
BsaHI	GRCGYC	2 cut(s)	80, 144
BsaJI	CCNNGG	3 cut(s)	903, 1116, 1549
BsaWI	WCCGGW	2 cut(s)	669, 1355
Bsc4I	CCNNNNNNNGG	3 cut(s)	519, 552, 745
Bse3DI	GCAATG	2 cut(s)	639, 1725
Bse8I	GATNNNNATC	1 cut(s)	362
BseAI	TCCGGA	1 cut(s)	1355
BseBI	CCWGG	4 cut(s)	59, 927, 1458, 1839
BseDI	CCNNGG	3 cut(s)	903, 1116, 1549
BseGI	GGATG	5 cut(s)	312, 678, 754, 1264, 1610
BseJI	GATNNNNATC	1 cut(s)	362
BseLI	CCNNNNNNNGG	3 cut(s)	519, 552, 745
BseMI	GCAATG	2 cut(s)	639, 1725
BseMII	CTCAG	4 cut(s)	464, 532, 593, 989
BseRI	GAGGAG	3 cut(s)	421, 804, 1307
BseSI	GKGCMC	1 cut(s)	1391
BseXI	GCAGC	6 cut(s)	212, 338, 585, 660, 720, 1749
BseYI	CCCAGC	1 cut(s)	576
BsgI	GTGCAG	1 cut(s)	1523
BshFI	GGCC	6 cut(s)	165, 381, 512, 930, 1677, 1772
BshNI	GGYRCC	1 cut(s)	79
BsiHKAI	GWGCWC	5 cut(s)	14, 498, 1068, 1391, 1926
BsiSI	CCGG	4 cut(s)	98, 618, 670, 1356
BslFI	GGGAC	2 cut(s)	17, 1652
BslI	CCNNNNNNNGG	3 cut(s)	519, 552, 745
BsmAI	GTCTC	2 cut(s)	135, 1736
BsmBI	CGTCTC	1 cut(s)	135
BsmFI	GGGAC	2 cut(s)	17, 1652
BsmI	GAATGC	1 cut(s)	612
BsnI	GGCC	6 cut(s)	165, 381, 512, 930, 1677, 1772
Bsp1286I	GDGCHC	5 cut(s)	14, 498, 1068, 1391, 1926
Bsp13I	TCCGGA	1 cut(s)	1355
Bsp1407I	TGTACA	1 cut(s)	1668
Bsp1720I	GCTNAGC	1 cut(s)	455
Bsp19I	CCATGG	1 cut(s)	903
BspACI	CCGC	9 cut(s)	66, 69, 156, 162, 202, 246, 594, 1630, 1859
BspANI	GGCC	6 cut(s)	165, 381, 512, 930, 1677, 1772
BspCNI	CTCAG	4 cut(s)	465, 531, 594, 990
BspEI	TCCGGA	1 cut(s)	1355
BspLI	GGNNCC	3 cut(s)	33, 81, 1462
BspMAI	CTGCAG	1 cut(s)	650
BspOI	GCTAGC	1 cut(s)	234
BspPI	GGATC	5 cut(s)	1167, 1331, 1455, 1468, 1829
BspQI	GCTCTTC	2 cut(s)	1056, 1813
BspT107I	GGYRCC	1 cut(s)	79
BsrDI	GCAATG	2 cut(s)	639, 1725
BsrGI	TGTACA	1 cut(s)	1668
BssECI	CCNNGG	3 cut(s)	903, 1116, 1549
BssNAI	GTATAC	1 cut(s)	1762
BssNI	GRCGYC	2 cut(s)	80, 144
BssSI	CACGAG	1 cut(s)	786
BssT1I	CCWWGG	1 cut(s)	903
Bst1107I	GTATAC	1 cut(s)	1762
Bst2BI	CACGAG	1 cut(s)	786
Bst2UI	CCWGG	4 cut(s)	59, 927, 1458, 1839
Bst4CI	ACNGT	8 cut(s)	40, 974, 1007, 1092, 1148, 1252, 1755, 1904
Bst6I	CTCTTC	6 cut(s)	393, 398, 882, 1056, 1813, 1865
BstACI	GRCGYC	2 cut(s)	80, 144
BstAPI	GCANNNNNTGC	1 cut(s)	645
BstAUI	TGTACA	1 cut(s)	1668
BstBAI	YACGTR	1 cut(s)	1543
BstC8I	GCNNGC	9 cut(s)	128, 148, 160, 232, 250, 646, 954, 958, 1308
BstDEI	CTNAG	7 cut(s)	329, 455, 473, 518, 602, 998, 1181
BstDSI	CCRYGG	2 cut(s)	903, 1549
BstF5I	GGATG	5 cut(s)	312, 678, 754, 1264, 1610
BstH2I	RGCGCY	1 cut(s)	83
BstHHI	GCGC	3 cut(s)	28, 82, 230
BstMAI	GTCTC	2 cut(s)	135, 1736
BstMWI	GCNNNNNNNGC	9 cut(s)	77, 183, 210, 225, 231, 493, 607, 645, 1303
BstNI	CCWGG	4 cut(s)	59, 927, 1458, 1839
BstNSI	RCATGY	2 cut(s)	130, 1669
BstSCI	CCNGG	5 cut(s)	57, 616, 925, 1456, 1837
BstSFI	CTRYAG	2 cut(s)	646, 689
BstSLI	GKGCMC	1 cut(s)	1391
BstV1I	GCAGC	6 cut(s)	212, 338, 585, 660, 720, 1749
BstX2I	RGATCY	1 cut(s)	1460
BstYI	RGATCY	1 cut(s)	1460
BstZ17I	GTATAC	1 cut(s)	1762
BsuRI	GGCC	6 cut(s)	165, 381, 512, 930, 1677, 1772
BtgI	CCRYGG	2 cut(s)	903, 1549
BtrI	CACGTC	1 cut(s)	181
BtsCI	GGATG	5 cut(s)	312, 678, 754, 1264, 1610
BtsI	GCAGTG	3 cut(s)	779, 1535, 1625
BtsIMutI	CAGTG	6 cut(s)	779, 1144, 1535, 1614, 1625, 1861
Cac8I	GCNNGC	9 cut(s)	128, 148, 160, 232, 250, 646, 954, 958, 1308
CfoI	GCGC	3 cut(s)	28, 82, 230
Cfr13I	GGNCC	3 cut(s)	31, 591, 1841
CseI	GACGC	1 cut(s)	152
Csp6I	GTAC	5 cut(s)	523, 794, 1204, 1669, 1928
CviQI	GTAC	5 cut(s)	523, 794, 1204, 1669, 1928
DdeI	CTNAG	7 cut(s)	329, 455, 473, 518, 602, 998, 1181
DinI	GGCGCC	1 cut(s)	81
DrdI	GACNNNNNNGTC	1 cut(s)	1373
DseDI	GACNNNNNNGTC	1 cut(s)	1373
EaeI	YGGCCR	1 cut(s)	163
Eam1104I	CTCTTC	6 cut(s)	393, 398, 882, 1056, 1813, 1865
EarI	CTCTTC	6 cut(s)	393, 398, 882, 1056, 1813, 1865
EciI	GGCGGA	1 cut(s)	55
Ecl136II	GAGCTC	3 cut(s)	12, 496, 1066
Eco130I	CCWWGG	1 cut(s)	903
Eco147I	AGGCCT	1 cut(s)	930
Eco24I	GRGCYC	3 cut(s)	14, 498, 1068
Eco32I	GATATC	2 cut(s)	1141, 1164
Eco47I	GGWCC	3 cut(s)	31, 591, 1841
Eco53kI	GAGCTC	3 cut(s)	12, 496, 1066
Eco57I	CTGAAG	1 cut(s)	764
Eco72I	CACGTG	1 cut(s)	1543
EcoICRI	GAGCTC	3 cut(s)	12, 496, 1066
EcoRII	CCWGG	4 cut(s)	57, 925, 1456, 1837
EcoRV	GATATC	2 cut(s)	1141, 1164
EcoT14I	CCWWGG	1 cut(s)	903
EcoT22I	ATGCAT	1 cut(s)	128
EcoT38I	GRGCYC	3 cut(s)	14, 498, 1068
EgeI	GGCGCC	1 cut(s)	81
EheI	GGCGCC	1 cut(s)	81
ErhI	CCWWGG	1 cut(s)	903
Esp3I	CGTCTC	1 cut(s)	135
FalI	AAGNNNNNCTT	2 cut(s)	1637, 1669
FaqI	GGGAC	2 cut(s)	17, 1652
FauI	CCCGC	1 cut(s)	253
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	1 cut(s)	1761
Fnu4HI	GCNGC	9 cut(s)	69, 163, 226, 352, 574, 649, 734, 1738, 1935
FokI	GGATG	5 cut(s)	319, 685, 761, 1271, 1597
FriOI	GRGCYC	3 cut(s)	14, 498, 1068
Fsp4HI	GCNGC	9 cut(s)	69, 163, 226, 352, 574, 649, 734, 1738, 1935
FspBI	CTAG	4 cut(s)	231, 563, 1209, 1224
GlaI	GCGC	3 cut(s)	27, 81, 229
GluI	GCNGC	9 cut(s)	69, 163, 226, 352, 574, 649, 734, 1738, 1935
GsaI	CCCAGC	1 cut(s)	580
GsuI	CTGGAG	1 cut(s)	1101
HaeII	RGCGCY	1 cut(s)	83
HaeIII	GGCC	6 cut(s)	165, 381, 512, 930, 1677, 1772
HapII	CCGG	4 cut(s)	98, 618, 670, 1356
HgaI	GACGC	1 cut(s)	152
HhaI	GCGC	3 cut(s)	28, 82, 230
Hin1I	GRCGYC	2 cut(s)	80, 144
Hin6I	GCGC	3 cut(s)	26, 80, 228
HinP1I	GCGC	3 cut(s)	26, 80, 228
HincII	GTYRAC	1 cut(s)	1126
HindII	GTYRAC	1 cut(s)	1126
HindIII	AAGCTT	2 cut(s)	485, 958
HinfI	GANTC	4 cut(s)	62, 299, 984, 1000
HpaI	GTTAAC	1 cut(s)	1126
HpaII	CCGG	4 cut(s)	98, 618, 670, 1356
HphI	GGTGA	5 cut(s)	145, 188, 232, 632, 1019
Hpy166II	GTNNAC	5 cut(s)	712, 1126, 1206, 1389, 1762
Hpy188I	TCNGA	9 cut(s)	49, 120, 140, 306, 336, 474, 766, 1275, 1813
Hpy188III	TCNNGA	6 cut(s)	208, 491, 1055, 1170, 1356, 1777
Hpy8I	GTNNAC	5 cut(s)	712, 1126, 1206, 1389, 1762
Hpy99I	CGWCG	3 cut(s)	95, 870, 1835
HpyAV	CCTTC	6 cut(s)	471, 739, 753, 908, 1350, 1420
HpyCH4III	ACNGT	8 cut(s)	40, 974, 1007, 1092, 1148, 1252, 1755, 1904
HpyCH4IV	ACGT	3 cut(s)	180, 1542, 1641
HpyF10VI	GCNNNNNNNGC	9 cut(s)	77, 183, 210, 225, 231, 493, 607, 645, 1303
HpyF3I	CTNAG	7 cut(s)	329, 455, 473, 518, 602, 998, 1181
HpySE526I	ACGT	3 cut(s)	180, 1542, 1641
Hsp92I	GRCGYC	2 cut(s)	80, 144
HspAI	GCGC	3 cut(s)	26, 80, 228
KasI	GGCGCC	1 cut(s)	79
Kpn2I	TCCGGA	1 cut(s)	1355
Ksp22I	TGATCA	1 cut(s)	622
KspAI	GTTAAC	1 cut(s)	1126
LguI	GCTCTTC	2 cut(s)	1056, 1813
LmnI	GCTCC	6 cut(s)	239, 418, 731, 741, 1294, 1314
Lsp1109I	GCAGC	6 cut(s)	212, 338, 585, 660, 720, 1749
LweI	GCATC	4 cut(s)	213, 739, 1104, 1619
MaeI	CTAG	4 cut(s)	231, 563, 1209, 1224
MaeII	ACGT	3 cut(s)	180, 1542, 1641
MaeIII	GTNAC	5 cut(s)	778, 917, 1001, 1229, 1246
MboII	GAAGA	9 cut(s)	325, 410, 415, 899, 947, 1073, 1702, 1800, 1882
MflI	RGATCY	1 cut(s)	1460
MhlI	GDGCHC	5 cut(s)	14, 498, 1068, 1391, 1926
MluCI	AATT	8 cut(s)	262, 316, 338, 871, 1046, 1109, 1380, 1580
Mly113I	GGCGCC	1 cut(s)	80
MlyI	GAGTC	3 cut(s)	56, 993, 1009
MmeI	TCCRAC	3 cut(s)	314, 763, 1847
Mph1103I	ATGCAT	1 cut(s)	128
MroI	TCCGGA	1 cut(s)	1355
MroXI	GAANNNNTTC	1 cut(s)	939
MseI	TTAA	4 cut(s)	834, 1125, 1413, 1917
MslI	CAYNNNNRTG	2 cut(s)	1421, 1509
MspA1I	CMGCKG	1 cut(s)	576
MspI	CCGG	4 cut(s)	98, 618, 670, 1356
MspR9I	CCNGG	5 cut(s)	59, 618, 927, 1458, 1839
Mva1269I	GAATGC	1 cut(s)	612
MvaI	CCWGG	4 cut(s)	59, 927, 1458, 1839
MwoI	GCNNNNNNNGC	9 cut(s)	77, 183, 210, 225, 231, 493, 607, 645, 1303
NarI	GGCGCC	1 cut(s)	80
NciI	CCSGG	1 cut(s)	618
NcoI	CCATGG	1 cut(s)	903
NheI	GCTAGC	1 cut(s)	230
NlaIV	GGNNCC	3 cut(s)	33, 81, 1462
NmeAIII	GCCGAG	1 cut(s)	457
NmuCI	GTSAC	2 cut(s)	1001, 1229
NsiI	ATGCAT	1 cut(s)	128
NspI	RCATGY	2 cut(s)	130, 1669
PaeI	GCATGC	1 cut(s)	130
PceI	AGGCCT	1 cut(s)	930
PciSI	GCTCTTC	2 cut(s)	1056, 1813
PcsI	WCGNNNNNNNCGW	1 cut(s)	1134
PctI	GAATGC	1 cut(s)	612
PdmI	GAANNNNTTC	1 cut(s)	939
PfeI	GAWTC	1 cut(s)	299
PfoI	TCCNGGA	3 cut(s)	57, 1456, 1837
PkrI	GCNGC	9 cut(s)	70, 164, 227, 353, 575, 650, 735, 1739, 1936
PleI	GAGTC	3 cut(s)	56, 992, 1008
PluTI	GGCGCC	1 cut(s)	83
PmaCI	CACGTG	1 cut(s)	1543
PmlI	CACGTG	1 cut(s)	1543
PpsI	GAGTC	3 cut(s)	56, 992, 1008
Ppu21I	YACGTR	1 cut(s)	1543
Psp124BI	GAGCTC	3 cut(s)	14, 498, 1068
Psp6I	CCWGG	4 cut(s)	57, 925, 1456, 1837
PspCI	CACGTG	1 cut(s)	1543
PspFI	CCCAGC	1 cut(s)	576
PspGI	CCWGG	4 cut(s)	57, 925, 1456, 1837
PspN4I	GGNNCC	3 cut(s)	33, 81, 1462
PspPI	GGNCC	3 cut(s)	31, 591, 1841
PstI	CTGCAG	1 cut(s)	650
PsuI	RGATCY	1 cut(s)	1460
PvuII	CAGCTG	1 cut(s)	576
RsaI	GTAC	5 cut(s)	524, 795, 1205, 1670, 1929
RsaNI	GTAC	5 cut(s)	523, 794, 1204, 1669, 1928
RseI	CAYNNNNRTG	2 cut(s)	1421, 1509
SacI	GAGCTC	3 cut(s)	14, 498, 1068
SapI	GCTCTTC	2 cut(s)	1056, 1813
SaqAI	TTAA	4 cut(s)	834, 1125, 1413, 1917
SatI	GCNGC	9 cut(s)	69, 163, 226, 352, 574, 649, 734, 1738, 1935
Sau96I	GGNCC	3 cut(s)	31, 591, 1841
ScaI	AGTACT	1 cut(s)	795
SchI	GAGTC	3 cut(s)	56, 993, 1009
ScrFI	CCNGG	5 cut(s)	59, 618, 927, 1458, 1839
SduI	GDGCHC	5 cut(s)	14, 498, 1068, 1391, 1926
SfaNI	GCATC	4 cut(s)	213, 739, 1104, 1619
SfcI	CTRYAG	2 cut(s)	646, 689
SfoI	GGCGCC	1 cut(s)	81
SinI	GGWCC	3 cut(s)	31, 591, 1841
SmiMI	CAYNNNNRTG	2 cut(s)	1421, 1509
SmlI	CTYRAG	2 cut(s)	1189, 1516
SmoI	CTYRAG	2 cut(s)	1189, 1516
SphI	GCATGC	1 cut(s)	130
Sse9I	AATT	8 cut(s)	262, 316, 338, 871, 1046, 1109, 1380, 1580
SseBI	AGGCCT	1 cut(s)	930
SsiI	CCGC	9 cut(s)	66, 69, 156, 162, 202, 246, 594, 1630, 1859
SspDI	GGCGCC	1 cut(s)	79
SspI	AATATT	1 cut(s)	1030
SspMI	CTAG	4 cut(s)	231, 563, 1209, 1224
SstI	GAGCTC	3 cut(s)	14, 498, 1068
StuI	AGGCCT	1 cut(s)	930
StyD4I	CCNGG	5 cut(s)	57, 616, 925, 1456, 1837
StyI	CCWWGG	1 cut(s)	903
TaaI	ACNGT	8 cut(s)	40, 974, 1007, 1092, 1148, 1252, 1755, 1904
TaiI	ACGT	3 cut(s)	183, 1545, 1644
TaqI	TCGA	5 cut(s)	273, 362, 976, 1419, 1778
TasI	AATT	8 cut(s)	262, 316, 338, 871, 1046, 1109, 1380, 1580
TatI	WGTACW	3 cut(s)	793, 1203, 1668
TauI	GCSGC	2 cut(s)	71, 165
TfiI	GAWTC	1 cut(s)	299
Tru1I	TTAA	4 cut(s)	834, 1125, 1413, 1917
Tru9I	TTAA	4 cut(s)	834, 1125, 1413, 1917
TscAI	CASTG	6 cut(s)	779, 1151, 1542, 1621, 1632, 1861
TseFI	GTSAC	2 cut(s)	1001, 1229
TseI	GCWGC	7 cut(s)	225, 351, 573, 648, 733, 1737, 1934
Tsp45I	GTSAC	2 cut(s)	1001, 1229
TspDTI	ATGAA	9 cut(s)	125, 1074, 1314, 1317, 1439, 1493, 1697, 1734, 1764
TspGWI	ACGGA	1 cut(s)	1538
TspRI	CASTG	6 cut(s)	779, 1151, 1542, 1621, 1632, 1861
VneI	GTGCAC	1 cut(s)	1387
VpaK11BI	GGWCC	3 cut(s)	31, 591, 1841
XapI	RAATTY	5 cut(s)	316, 1046, 1109, 1380, 1580
XceI	RCATGY	2 cut(s)	130, 1669
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1471
XmiI	GTMKAC	1 cut(s)	1761
XmnI	GAANNNNTTC	1 cut(s)	939
XspI	CTAG	4 cut(s)	231, 563, 1209, 1224
ZrmI	AGTACT	1 cut(s)	795
Zsp2I	ATGCAT	1 cut(s)	128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.