Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001361

Physical Location & Seq

Genomic Coordinates Forward (+)

4834417 .. 4841276

Gene Structure 6860 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1037700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGGATGAGTGCTCGACCGGGGCTCTAGTCCCAGCAGTGAAACCAGAACCAAAGGAGTCAAGCGTCGGGGATTTGGCAGATCCAGGCAGCGGTAACATGGGTTGTCCGGTGCATGAGAAAGGACTGGCGGAGGTCGACAGGGACTTGTTATGCCCAATTTGTATGCAGGTGGTAAAGGACGCCTTCCTCACAGCATGTGGTCATAGCTTCTGCTACATGTGCATCAAGACCCACCTCAGCAACAAGAGCGACTGCCCTTGCTGCGCCCAGTTCCTCAGCACCAACCAATTGTTCCCTAATTTTTTGCTGGACAAGCTTCTGAAGAAGACCTCAGCTCGTCAAATTTCCAAAAATGCATCCCCTGTAGAGCATTTTCACCAGGCATTGCACCAGGGATGTGAAGTGTCAATCAAGGAGCTAGACACCCTGTTGGCACTCCTGGCAGAGAAGAAGAGGAAAATGGAACAAGAAGAGGCGGAGAGAAACATGCAAATACTGCTTGACTTCTTGAATTGCCTAAGGAAGCAAAAAGTTGACGAGCTTAATGAGGTGCAAACCCATCTCCAATTTATCAAAGAGGACATAGGTGCAGTAGAGAGACGTAGAATGGAATTATACCGTGCCAGGGACAAATACTCTGTCAAGCTGCGGATGCTTGGAGCAGATGATTCTATTTATGGGGCAAGAAAGCAATGGCATTCCTCTACAGATAACAATACCGGTGCACTTAGTGGACGAGGAGGGATGTCAATTTGGAATCTTCCAAGAAAGGATGCATCAGACTCCCAGTATATGACTCAAACTGGTCTTGCCATAGCCAGAAAAAAGCGGGTCCATGCACAGTTTGGTGAACTTCAAGAGTGTTACCTGCAAAAGCGGCATCAGATGGTGAACCAACCATATTCCCAGCAAGAACAGGACAAAGGTGTAATACAAAGAGAAGGTTATACTGCAGGTCTTGCTGATTTTCAAACAGTGCTCACTACGTTAACACGTTACAGTCGAATGAGGGTCATTGCTGAACTTAGGCATGGGGATCTATTTCACTCAGCCAATATAGTATCCAGCATTGAATTTGACTGTGATTATGAGTTATTTGCTACTGCCGGAGTATCACGGTGCATAAAAGTTTTTGACTTCTCTTCGGTTTTGAATGATCCAGCTGACGTGCACTGTCCTGTTGTGGAGATGCCTACACGTTCAAAGCTTAGTTGCTTGAGCTGGAACAAGTTTACTAAAAACCATATAGCCAGTACTGATTATGAGGGAATAGTAACAGTTTGGGATGTAAATACCCGGCAGAGTATCATGGAATATGAAGAGCATGAAAAACGTGCTTGGAGTGTTGATTTTTCATGCACAGAGCCCACAAGGCTTGTATCTGGTAGTGATGATTGTAAGTTAAAACTTTGGTGCACAAGGCAGGAAGCTAGCGTTATTGATATTGACATGAAAGCCAACATATGTTCCGTCAAGTATAATCCAGGATCTGGAAACTGCATTGCGGTTGGTTCAGCAGACCATCACATCCACTATTATGATTTAAGAAATCCGAGCGAACCACTCCATGTGTTCACTGGGCATGGGAAAGCTGTTTCTTATGTAAAGTTCTTGTCAAACTATGAGCTTGCCTCTGCATCCACTGATAGCACATTGCGGTTATGGAATGTGAGGGATAATATTCCAGTTCGTACTTTCAAAGGTCACACAAATGAGAAGAACTTTGTAGGTCTTACAGTAAACAGGGAATACATTGCATGTGGCAGCGAAACAAATGAAGTGTTCGTGTATCATAAGGAAATCTCTAAACCGGTGACTAGGCATAAGTTTGGGTCGCCTGATTTGGATGACGCTGATGATGATGCGGGGTCGTACTTCATCAGTGCTGTATGTTGGAAGAGTGATAGCCCTACCATGCTAACTGCTAACAGTCAAGGAACCATTAAAGTGTTGGTTCTTGCGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009791 GO:0009812 GO:0009987 GO:0010119 GO:0010228 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40 zf-C3HC4_2

Protein Analysis

656

Amino Acids

73.68

Weight (kDa)

6.38

Isoelectric Point (pI)

42.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	48 - 90	8e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	51 - 88	8.9e-06	Ring finger domain
zf-C3HC4	PF00097	51 - 88	1e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	51 - 88	6.7e-10	Zinc finger, C3HC4 type (RING finger)
Beta-prop_WDR5	PF25175	351 - 602	2.3e-32	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	352 - 514	2.1e-12	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	352 - 461	3.6e-07	RIG second beta-propeller
Beta-prop_THOC3	PF25174	400 - 517	4.5e-17	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	402 - 495	5.9e-10	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	402 - 478	2.7e-06	Prp19 WD40 domain
WD40_Gbeta	PF25391	403 - 520	1.1e-06	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	412 - 537	1.5e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	414 - 574	3.6e-07	MABP1/WDR62 second WD40 domain
Beta-prop_EML	PF23409	419 - 601	6.8e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	440 - 586	4.7e-09	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	457 - 576	1.2e-11	WDHD1 first WD40 domain
WD40_Prp19	PF24814	485 - 608	8.5e-13	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	486 - 572	3.6e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	486 - 597	6.3e-13	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	498 - 605	7e-08	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	524 - 652	1.2e-11	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32950 AT2G32950
fragaria_vesca	FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_5g22570 FvH4_5g22570
malus_domestica	MD06G1037700.v1.1 MD10G1328500.v1.1 MD15G1405100.v1.1 MD15G1405200.v1.1 MD16G1272200.v1.1
prunus_persica	Prupe.4G013500_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1
pyrus_communis	pycom10g27830 pycom16g24110
rosa_chinensis	RchiOBHm_Chr5g0001791 RchiOBHm_Chr7g0210341
rosa_laevigata	RLG00000003045 RLG00000030973
rosa_multiflora	Rmu_co8137394.1_g000001 Rmu_sc0003807.1_g000002 Rmu_ssc0000237.1_g000012
rosa_roxburghii	Rroxscaffold_1G00071250 Rroxscaffold_1G00074540 Rroxscaffold_3G00248380
rosa_rugosa	Rorug04G0393100 Rorug04G0393200 Rorug04G0393300 Rorug07G0121200 Rorug07G0121200 Rorug07G0121300
rosa_samantha	Rh5AG014400 Rh5BG017800 Rh5CG015500 Rh5DG015900 Rh7AG253900 Rh7BG248800 Rh7CG270700 Rh7DG261400
rosa_wichuraiana	Rw5G001390 Rw7G021640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	157
Acc36I	ACCTGC	3 cut(s)	157, 876, 944
AccB7I	CCANNNNNTGG	1 cut(s)	1490
AccI	GTMKAC	1 cut(s)	135
AclWI	GGATC	4 cut(s)	74, 1044, 1151, 1495
AcsI	RAATTY	2 cut(s)	342, 1073
AcuI	CTGAAG	1 cut(s)	341
AcyI	GRCGYC	1 cut(s)	180
AdeI	CACNNNGTG	1 cut(s)	731
AfaI	GTAC	3 cut(s)	1255, 1693, 1874
AfiI	CCNNNNNNNGG	3 cut(s)	89, 625, 1490
AflIII	ACRYGT	3 cut(s)	216, 992, 1196
AgeI	ACCGGT	2 cut(s)	719, 1810
AgsI	TTSAA	7 cut(s)	511, 857, 971, 1073, 1153, 1203, 1699
AjiI	CACGTC	1 cut(s)	1168
AjnI	CCWGG	6 cut(s)	82, 378, 390, 438, 623, 1483
AjuI	GAANNNNNNNTTGG	2 cut(s)	275, 307
Alw21I	GWGCWC	5 cut(s)	14, 727, 981, 1173, 1418
Alw26I	GTCTC	1 cut(s)	592
Alw44I	GTGCAC	3 cut(s)	723, 1169, 1414
AlwI	GGATC	4 cut(s)	74, 1044, 1151, 1495
AlwNI	CAGNNNCTG	1 cut(s)	1490
ApaLI	GTGCAC	3 cut(s)	723, 1169, 1414
ApeKI	GCWGC	4 cut(s)	87, 261, 646, 1764
ApoI	RAATTY	2 cut(s)	342, 1073
ArsI	GACNNNNNNTTYG	2 cut(s)	624, 656
AsiGI	ACCGGT	2 cut(s)	719, 1810
AspLEI	GCGC	1 cut(s)	266
AspS9I	GGNCC	1 cut(s)	832
AsuC2I	CCSGG	2 cut(s)	19, 1297
AsuHPI	GGTGA	4 cut(s)	368, 860, 901, 1825
AsuNHI	GCTAGC	1 cut(s)	1430
AvaII	GGWCC	1 cut(s)	832
AxyI	CCTNAGG	1 cut(s)	518
BaeGI	GKGCMC	3 cut(s)	727, 1173, 1418
BanII	GRGCYC	2 cut(s)	25, 1368
BbsI	GAAGAC	1 cut(s)	332
Bbv12I	GWGCWC	5 cut(s)	14, 727, 981, 1173, 1418
BbvCI	CCTCAGC	3 cut(s)	236, 275, 331
BbvI	GCAGC	4 cut(s)	99, 248, 633, 1776
BccI	CCATC	3 cut(s)	567, 880, 1530
BciT130I	CCWGG	6 cut(s)	84, 380, 392, 440, 625, 1485
BciVI	GTATCC	1 cut(s)	1072
BcnI	CCSGG	2 cut(s)	19, 1297
BcoDI	GTCTC	1 cut(s)	592
BfaI	CTAG	4 cut(s)	26, 419, 1431, 1818
BfmI	CTRYAG	3 cut(s)	363, 705, 951
BfuAI	ACCTGC	3 cut(s)	157, 876, 944
BfuI	GTATCC	1 cut(s)	1072
BglI	GCCNNNNNGGC	1 cut(s)	1372
BisI	GCNGC	6 cut(s)	88, 262, 647, 878, 1765, 1962
BlsI	GCNGC	6 cut(s)	89, 263, 648, 879, 1766, 1963
BmcAI	AGTACT	1 cut(s)	1255
Bme1390I	CCNGG	8 cut(s)	19, 84, 380, 392, 440, 625, 1297, 1485
Bme18I	GGWCC	1 cut(s)	832
BmgBI	CACGTC	1 cut(s)	1168
BmgT120I	GGNCC	1 cut(s)	832
BmiI	GGNNCC	2 cut(s)	833, 1939
BmrFI	CCNGG	8 cut(s)	19, 84, 380, 392, 440, 625, 1297, 1485
BmrI	ACTGGG	3 cut(s)	262, 781, 1587
BmsI	GCATC	9 cut(s)	231, 365, 642, 763, 785, 889, 1179, 1646, 1852
BmtI	GCTAGC	1 cut(s)	1434
BmuI	ACTGGG	3 cut(s)	262, 781, 1587
BpiI	GAAGAC	1 cut(s)	332
BplI	GAGNNNNNCTC	2 cut(s)	1616, 1648
Bpu10I	CCTNAGC	3 cut(s)	236, 275, 331
BpuEI	CTTGAG	1 cut(s)	1237
BpuMI	CCSGG	2 cut(s)	19, 1297
BsaHI	GRCGYC	1 cut(s)	180
BsaJI	CCNNGG	3 cut(s)	18, 391, 624
BsaWI	WCCGGW	3 cut(s)	106, 719, 1810
Bsc4I	CCNNNNNNNGG	3 cut(s)	89, 625, 1490
Bse118I	RCCGGY	2 cut(s)	719, 1810
Bse1I	ACTGG	7 cut(s)	129, 268, 787, 808, 1251, 1582, 1685
Bse21I	CCTNAGG	1 cut(s)	518
Bse3DI	GCAATG	6 cut(s)	383, 698, 1014, 1500, 1652, 1752
BseBI	CCWGG	6 cut(s)	84, 380, 392, 440, 625, 1485
BseDI	CCNNGG	3 cut(s)	18, 391, 624
BseLI	CCNNNNNNNGG	3 cut(s)	89, 625, 1490
BseMI	GCAATG	6 cut(s)	383, 698, 1014, 1500, 1652, 1752
BseMII	CTCAG	4 cut(s)	250, 289, 345, 1062
BseNI	ACTGG	7 cut(s)	129, 268, 787, 808, 1251, 1582, 1685
BseRI	GAGGAG	1 cut(s)	753
BseSI	GKGCMC	3 cut(s)	727, 1173, 1418
BseXI	GCAGC	4 cut(s)	99, 248, 633, 1776
BseYI	CCCAGC	2 cut(s)	31, 906
BsgI	GTGCAG	1 cut(s)	609
Bsh1285I	CGRYCG	1 cut(s)	18
BshTI	ACCGGT	2 cut(s)	719, 1810
BsiEI	CGRYCG	1 cut(s)	18
BsiHKAI	GWGCWC	5 cut(s)	14, 727, 981, 1173, 1418
BsiSI	CCGG	6 cut(s)	18, 107, 720, 1107, 1297, 1811
BslFI	GGGAC	3 cut(s)	14, 155, 641
BslI	CCNNNNNNNGG	3 cut(s)	89, 625, 1490
BsmAI	GTCTC	1 cut(s)	592
BsmBI	CGTCTC	1 cut(s)	592
BsmFI	GGGAC	3 cut(s)	14, 155, 641
BsmI	GAATGC	1 cut(s)	697
Bsp1286I	GDGCHC	7 cut(s)	14, 25, 727, 981, 1173, 1368, 1418
Bsp143I	GATC	4 cut(s)	79, 1036, 1156, 1487
BspCNI	CTCAG	4 cut(s)	249, 288, 344, 1061
BspLI	GGNNCC	2 cut(s)	833, 1939
BspMAI	CTGCAG	1 cut(s)	955
BspMI	ACCTGC	3 cut(s)	157, 876, 944
BspOI	GCTAGC	1 cut(s)	1434
BspPI	GGATC	4 cut(s)	74, 1044, 1151, 1495
BspQI	GCTCTTC	1 cut(s)	1314
BsrDI	GCAATG	6 cut(s)	383, 698, 1014, 1500, 1652, 1752
BsrFI	RCCGGY	2 cut(s)	719, 1810
BsrI	ACTGG	7 cut(s)	129, 268, 787, 808, 1251, 1582, 1685
BssAI	RCCGGY	2 cut(s)	719, 1810
BssECI	CCNNGG	3 cut(s)	18, 391, 624
BssMI	GATC	4 cut(s)	79, 1036, 1156, 1487
BssNI	GRCGYC	1 cut(s)	180
Bst2UI	CCWGG	6 cut(s)	84, 380, 392, 440, 625, 1485
Bst6I	CTCTTC	5 cut(s)	446, 465, 1147, 1314, 1892
BstACI	GRCGYC	1 cut(s)	180
BstAPI	GCANNNNNTGC	2 cut(s)	496, 959
BstC8I	GCNNGC	2 cut(s)	1432, 1629
BstDEI	CTNAG	8 cut(s)	236, 275, 331, 518, 728, 1025, 1048, 1208
BstHHI	GCGC	1 cut(s)	266
BstKTI	GATC	4 cut(s)	82, 1039, 1159, 1490
BstMAI	GTCTC	1 cut(s)	592
BstMBI	GATC	4 cut(s)	79, 1036, 1156, 1487
BstMCI	CGRYCG	1 cut(s)	18
BstNI	CCWGG	6 cut(s)	84, 380, 392, 440, 625, 1485
BstNSI	RCATGY	4 cut(s)	198, 220, 490, 1761
BstSCI	CCNGG	8 cut(s)	17, 82, 378, 390, 438, 623, 1295, 1483
BstSFI	CTRYAG	3 cut(s)	363, 705, 951
BstSLI	GKGCMC	3 cut(s)	727, 1173, 1418
BstV1I	GCAGC	4 cut(s)	99, 248, 633, 1776
BstV2I	GAAGAC	1 cut(s)	332
BstX2I	RGATCY	3 cut(s)	79, 1036, 1487
BstYI	RGATCY	3 cut(s)	79, 1036, 1487
Bsu36I	CCTNAGG	1 cut(s)	518
BsuI	GTATCC	1 cut(s)	1072
BtrI	CACGTC	1 cut(s)	1168
BtsI	GCAGTG	1 cut(s)	42
BtsIMutI	CAGTG	6 cut(s)	42, 981, 1171, 1575, 1641, 1888
BveI	ACCTGC	3 cut(s)	157, 876, 944
Cac8I	GCNNGC	2 cut(s)	1432, 1629
CaiI	CAGNNNCTG	1 cut(s)	1490
CfoI	GCGC	1 cut(s)	266
Cfr10I	RCCGGY	2 cut(s)	719, 1810
Cfr13I	GGNCC	1 cut(s)	832
CseI	GACGC	3 cut(s)	52, 188, 1859
Csp6I	GTAC	3 cut(s)	1254, 1692, 1873
CspAI	ACCGGT	2 cut(s)	719, 1810
CspCI	CAANNNNNGTGG	2 cut(s)	1357, 1392
CviQI	GTAC	3 cut(s)	1254, 1692, 1873
DdeI	CTNAG	8 cut(s)	236, 275, 331, 518, 728, 1025, 1048, 1208
DpnI	GATC	4 cut(s)	81, 1038, 1158, 1489
DpnII	GATC	4 cut(s)	79, 1036, 1156, 1487
DraIII	CACNNNGTG	1 cut(s)	731
Eam1104I	CTCTTC	5 cut(s)	446, 465, 1147, 1314, 1892
EarI	CTCTTC	5 cut(s)	446, 465, 1147, 1314, 1892
EciI	GGCGGA	2 cut(s)	143, 491
Eco24I	GRGCYC	2 cut(s)	25, 1368
Eco47I	GGWCC	1 cut(s)	832
Eco57I	CTGAAG	1 cut(s)	341
Eco81I	CCTNAGG	1 cut(s)	518
EcoRII	CCWGG	6 cut(s)	82, 378, 390, 438, 623, 1483
EcoT22I	ATGCAT	2 cut(s)	358, 778
EcoT38I	GRGCYC	2 cut(s)	25, 1368
Esp3I	CGTCTC	1 cut(s)	592
FalI	AAGNNNNNCTT	2 cut(s)	167, 199
FaqI	GGGAC	3 cut(s)	14, 155, 641
FauI	CCCGC	2 cut(s)	822, 1858
FauNDI	CATATG	1 cut(s)	1463
FblI	GTMKAC	1 cut(s)	135
Fnu4HI	GCNGC	6 cut(s)	88, 262, 647, 878, 1765, 1962
FriOI	GRGCYC	2 cut(s)	25, 1368
Fsp4HI	GCNGC	6 cut(s)	88, 262, 647, 878, 1765, 1962
FspBI	CTAG	4 cut(s)	26, 419, 1431, 1818
GlaI	GCGC	1 cut(s)	265
GluI	GCNGC	6 cut(s)	88, 262, 647, 878, 1765, 1962
GsaI	CCCAGC	2 cut(s)	35, 910
HapII	CCGG	6 cut(s)	18, 107, 720, 1107, 1297, 1811
HgaI	GACGC	3 cut(s)	52, 188, 1859
HhaI	GCGC	1 cut(s)	266
Hin1I	GRCGYC	1 cut(s)	180
Hin6I	GCGC	1 cut(s)	264
HinP1I	GCGC	1 cut(s)	264
HincII	GTYRAC	3 cut(s)	136, 535, 990
HindII	GTYRAC	3 cut(s)	136, 535, 990
HindIII	AAGCTT	2 cut(s)	314, 1205
HinfI	GANTC	5 cut(s)	56, 668, 757, 782, 796
HpaI	GTTAAC	1 cut(s)	990
HpaII	CCGG	6 cut(s)	18, 107, 720, 1107, 1297, 1811
HphI	GGTGA	4 cut(s)	368, 860, 901, 1825
Hpy188I	TCNGA	4 cut(s)	321, 781, 885, 1554
Hpy188III	TCNNGA	4 cut(s)	226, 508, 857, 1491
Hpy99I	CGWCG	1 cut(s)	68
HpyAV	CCTTC	2 cut(s)	193, 935
HpyCH4IV	ACGT	6 cut(s)	601, 986, 994, 1167, 1198, 1333
HpyF3I	CTNAG	8 cut(s)	236, 275, 331, 518, 728, 1025, 1048, 1208
HpySE526I	ACGT	6 cut(s)	601, 986, 994, 1167, 1198, 1333
Hsp92I	GRCGYC	1 cut(s)	180
HspAI	GCGC	1 cut(s)	264
KspAI	GTTAAC	1 cut(s)	990
Kzo9I	GATC	4 cut(s)	79, 1036, 1156, 1487
LguI	GCTCTTC	1 cut(s)	1314
LmnI	GCTCC	2 cut(s)	415, 659
Lsp1109I	GCAGC	4 cut(s)	99, 248, 633, 1776
LweI	GCATC	9 cut(s)	231, 365, 642, 763, 785, 889, 1179, 1646, 1852
MaeI	CTAG	4 cut(s)	26, 419, 1431, 1818
MaeII	ACGT	6 cut(s)	601, 986, 994, 1167, 1198, 1333
MaeIII	GTNAC	6 cut(s)	92, 863, 995, 1273, 1703, 1813
MalI	GATC	4 cut(s)	81, 1038, 1158, 1489
MboI	GATC	4 cut(s)	79, 1036, 1156, 1487
MfeI	CAATTG	1 cut(s)	287
MflI	RGATCY	3 cut(s)	79, 1036, 1487
MhlI	GDGCHC	7 cut(s)	14, 25, 727, 981, 1173, 1368, 1418
MluCI	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 611, 750, 1073
MlyI	GAGTC	3 cut(s)	65, 776, 790
MmeI	TCCRAC	1 cut(s)	1874
Mph1103I	ATGCAT	2 cut(s)	358, 778
MseI	TTAA	5 cut(s)	543, 989, 1403, 1544, 1944
MslI	CAYNNNNRTG	3 cut(s)	1536, 1710, 1946
MspA1I	CMGCKG	2 cut(s)	90, 1163
MspI	CCGG	6 cut(s)	18, 107, 720, 1107, 1297, 1811
MspR9I	CCNGG	8 cut(s)	19, 84, 380, 392, 440, 625, 1297, 1485
MunI	CAATTG	1 cut(s)	287
Mva1269I	GAATGC	1 cut(s)	697
MvaI	CCWGG	6 cut(s)	84, 380, 392, 440, 625, 1485
NciI	CCSGG	2 cut(s)	19, 1297
NdeI	CATATG	1 cut(s)	1463
NdeII	GATC	4 cut(s)	79, 1036, 1156, 1487
NheI	GCTAGC	1 cut(s)	1430
NlaIV	GGNNCC	2 cut(s)	833, 1939
NmuCI	GTSAC	2 cut(s)	1703, 1813
NsiI	ATGCAT	2 cut(s)	358, 778
NspI	RCATGY	4 cut(s)	198, 220, 490, 1761
PaqCI	CACCTGC	1 cut(s)	157
PciI	ACATGT	1 cut(s)	216
PciSI	GCTCTTC	1 cut(s)	1314
PcsI	WCGNNNNNNNCGW	1 cut(s)	1000
PctI	GAATGC	1 cut(s)	697
PfeI	GAWTC	2 cut(s)	668, 757
PflMI	CCANNNNNTGG	1 cut(s)	1490
PfoI	TCCNGGA	1 cut(s)	1483
PinAI	ACCGGT	2 cut(s)	719, 1810
PkrI	GCNGC	6 cut(s)	89, 263, 648, 879, 1766, 1963
PleI	GAGTC	3 cut(s)	64, 776, 790
PpsI	GAGTC	3 cut(s)	64, 776, 790
PscI	ACATGT	1 cut(s)	216
Psp6I	CCWGG	6 cut(s)	82, 378, 390, 438, 623, 1483
PspFI	CCCAGC	2 cut(s)	31, 906
PspGI	CCWGG	6 cut(s)	82, 378, 390, 438, 623, 1483
PspN4I	GGNNCC	2 cut(s)	833, 1939
PspPI	GGNCC	1 cut(s)	832
PstI	CTGCAG	1 cut(s)	955
PstNI	CAGNNNCTG	1 cut(s)	1490
PsuI	RGATCY	3 cut(s)	79, 1036, 1487
PvuII	CAGCTG	1 cut(s)	1163
RsaI	GTAC	3 cut(s)	1255, 1693, 1874
RsaNI	GTAC	3 cut(s)	1254, 1692, 1873
RseI	CAYNNNNRTG	3 cut(s)	1536, 1710, 1946
SalI	GTCGAC	1 cut(s)	134
SapI	GCTCTTC	1 cut(s)	1314
SaqAI	TTAA	5 cut(s)	543, 989, 1403, 1544, 1944
SatI	GCNGC	6 cut(s)	88, 262, 647, 878, 1765, 1962
Sau3AI	GATC	4 cut(s)	79, 1036, 1156, 1487
Sau96I	GGNCC	1 cut(s)	832
ScaI	AGTACT	1 cut(s)	1255
SchI	GAGTC	3 cut(s)	65, 776, 790
ScrFI	CCNGG	8 cut(s)	19, 84, 380, 392, 440, 625, 1297, 1485
SduI	GDGCHC	7 cut(s)	14, 25, 727, 981, 1173, 1368, 1418
SfaNI	GCATC	9 cut(s)	231, 365, 642, 763, 785, 889, 1179, 1646, 1852
SfcI	CTRYAG	3 cut(s)	363, 705, 951
SinI	GGWCC	1 cut(s)	832
SmiMI	CAYNNNNRTG	3 cut(s)	1536, 1710, 1946
SmlI	CTYRAG	1 cut(s)	1216
SmoI	CTYRAG	1 cut(s)	1216
Sse9I	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 611, 750, 1073
SspI	AATATT	1 cut(s)	1681
SspMI	CTAG	4 cut(s)	26, 419, 1431, 1818
StyD4I	CCNGG	8 cut(s)	17, 82, 378, 390, 438, 623, 1295, 1483
TaiI	ACGT	6 cut(s)	604, 989, 997, 1170, 1201, 1336
TaqI	TCGA	3 cut(s)	14, 135, 1003
TasI	AATT	9 cut(s)	156, 287, 298, 342, 511, 566, 611, 750, 1073
TatI	WGTACW	1 cut(s)	1253
TauI	GCSGC	2 cut(s)	880, 1964
TfiI	GAWTC	2 cut(s)	668, 757
Tru1I	TTAA	5 cut(s)	543, 989, 1403, 1544, 1944
Tru9I	TTAA	5 cut(s)	543, 989, 1403, 1544, 1944
TscAI	CASTG	6 cut(s)	42, 981, 1178, 1582, 1648, 1888
TseFI	GTSAC	2 cut(s)	1703, 1813
TseI	GCWGC	4 cut(s)	87, 261, 646, 1764
Tsp45I	GTSAC	2 cut(s)	1703, 1813
TspDTI	ATGAA	6 cut(s)	1332, 1341, 1344, 1466, 1791, 1867
TspGWI	ACGGA	1 cut(s)	1459
TspRI	CASTG	6 cut(s)	42, 981, 1178, 1582, 1648, 1888
Van91I	CCANNNNNTGG	1 cut(s)	1490
VneI	GTGCAC	3 cut(s)	723, 1169, 1414
VpaK11BI	GGWCC	1 cut(s)	832
XapI	RAATTY	2 cut(s)	342, 1073
XceI	RCATGY	4 cut(s)	198, 220, 490, 1761
XcmI	CCANNNNNNNNNTGG	3 cut(s)	842, 1574, 1948
XmiI	GTMKAC	1 cut(s)	135
XspI	CTAG	4 cut(s)	26, 419, 1431, 1818
ZrmI	AGTACT	1 cut(s)	1255
Zsp2I	ATGCAT	2 cut(s)	358, 778

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.