Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001361

Physical Location & Seq

Genomic Coordinates Reverse (-)

41670964 .. 41678576

Gene Structure 7613 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00248380.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGGAGGAGTGTTCAACTGGGGCTCTAGTCCCGGCAGTGAAAGTGGAACCAATTCTATCAAGCATCGGGGGTTCGGTGGATCCATGCAGCAGTGACTTGGGTCGTCTGACGGAGGAGAAAGGAATGTCAGAGGTGGACAAGGATTTGCTGTGTCCAATTTGCATGCAGATTGTAAAGGACGCATTCCTCACAGCGTGCGGCCATAGCTTCTGCTATATGTGTATCATCACCCACCTTAGCAACAAGAGTGACTGCCCTTGCTGTGCCCAATCTCTCAGCAAAAACCAACTATTCCCCAATTTCTTACTGGACAAGCTTCTGAAGAAGACTTCAGCTCAACAGATTTCAAAAAGCGCATCCCCTGTGGAACATTTTCGTCAGGCATTGCAGCAGGGCTGTGAAGTGTCCATCAAGGAGCTAGACACCCTGTTGGCACTCCTTGCAGAGAAGAAGAGGAAAATGGAACAAGAAGAAGCCGAGAGAAATATGCAAATATTGCTTGACTTCTTGAATTGCCTAAGGAAGCAGAAAGTTCAAGAGCTTAATGAGGTACAGTCCCATCTTCAATTTCTCAAAGAGGACATAATTGTAGTTGAGAGACGCAGAATGGAGTTGTACCGTGCCAGGGATAGGTACTCTGTGAGGCAGCGGATGCTTGGAGGGGATGATTCTGTCAATGGTGCAAGAAATTCGTGGCCTTCCTCTGTAGACAACAACACCAGTGCTCTCAGAGTACGAGGAGGAACGTCCTCTTGGAATGTTCAGGGACGAGGACTGCAGAGAAATGATGCTTTAAGTGCATCAGACTCCCAGTATGTGAATCAATCAGGACTTGCTGTTGCCAGGAAAAAGCGGGTCCATGCATTGTTCGATGAGCTACAAGAGTGTTACCTGCAAAAGCGACGTCAGATGAACCAACCATATTCCCAGCTAGAACGGGACAGAACTGTAATACCCAGAGAAGGTTATAGTACAGGTCTTGCCGATTTTCAGTCAGTGCTTACTACGTTAACGCGCTACAGTCGAATGAGGGTCATTGCTGAACTTAGGCATGAGGATCTATTTCACTCGGCCAATATAGTATCAAGCATCGAATTTGACCGTGATTATGAGTTGTTTGCCACTGCTGGAGTTTCAAAGTGCATAAAAGTTTTTGACTTTTCCTCGGTTGTGAATGATCCAGCTGATATGCACACTGCTGTTGTTGAGATGCCTACACGATCAAAGCTAAGTTGCTTGAGCTGGAACAAGTTTACTAAAAACCATATAGCTAGTACTGATTATGAGGGAATAGTAACTGTCTGGGACGTAGATACTCGGCAGAGTGTCATGGAATATGAAGAGCATGAAAAACGTGCTTGGAGTGTTGATTTTTCATGCACAGAACCCTCGATGCTTGTATCCGGTAGTGATGATTGTAAGGTAAAGGTTTGGTGCACAAGGCAGGAAGCTAGTGTTCTTAACATTGACATGAAAGCAAACATATGTTGCGTGAAGTATAATCCTGGATCTAGCAACTGCATTGCGGTAGGTTCGGCAGATCATCACATTCACTATTATGATTTAAGAAATGTGAGTCAACCACTGCATGTGTTTACTGGGCACAGGAAAGCAGTTTCCTATGTGAAATTCTTGTCAAACTATGAGCTTGCGTCTGCATCTACTGATAGCACGTTGCGGTTATGGAATGTGAAGGATAATATTCCAGTTCGTACTTTTAAAGGTCACACAAATGAGAAGAACTTCGTAGGTCTTACGGTAAACAATGAATACATCGCATGTGGCAGCGAAACAAATGAAGTGTTCGTGTATCATAAGGAAATTTCTAAACCTGTGACTTGGCACAGATTTGGGTCACCTGATATGGAAGACACCGAAGATGATGCAGGGTCTTACTTCATCAGTGCTGTATGTTGGAAGAGTGATAGCCCTACAATGCTAACTGCTAACAGTCAGGGAACCATTAAGGTCCTCGTTCTTGCAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009791 GO:0009812 GO:0009987 GO:0010119 GO:0010228 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40 zf-C3HC4_2

Protein Analysis

662

Amino Acids

74.71

Weight (kDa)

6.34

Isoelectric Point (pI)

50.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	48 - 93	1.9e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	51 - 88	2.1e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	51 - 88	6.9e-10	Zinc finger, C3HC4 type (RING finger)
Beta-prop_WDR5	PF25175	358 - 607	1.1e-33	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	358 - 484	2.2e-10	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	358 - 469	1.5e-08	RIG second beta-propeller
Beta-prop_THOC3	PF25174	407 - 525	4.5e-17	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	409 - 502	1.9e-10	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	409 - 485	1.5e-07	Prp19 WD40 domain
WD40_Gbeta	PF25391	410 - 532	2.9e-06	G protein beta WD-40 repeat protein
Beta-prop_EML_2	PF23414	419 - 546	1.1e-12	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	420 - 596	1.5e-09	MABP1/WDR62 second WD40 domain
Beta-prop_EML	PF23409	426 - 607	3.5e-08	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	465 - 595	3e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_EIPR1	PF23609	485 - 562	2.6e-06	EIPR1 beta-propeller
WDR55	PF24796	488 - 658	6.1e-06	WDR55
WD40_WDHD1_1st	PF24817	491 - 584	2.4e-11	WDHD1 first WD40 domain
Beta-prop_TEP1_2nd	PF25047	491 - 580	1e-07	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	491 - 597	1.2e-14	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	492 - 614	1.9e-14	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	495 - 612	7.3e-09	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	530 - 658	2.5e-11	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32950 AT2G32950
fragaria_vesca	FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_5g22570 FvH4_5g22570
malus_domestica	MD06G1037700.v1.1 MD10G1328500.v1.1 MD15G1405100.v1.1 MD15G1405200.v1.1 MD16G1272200.v1.1
prunus_persica	Prupe.4G013500_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1
pyrus_communis	pycom10g27830 pycom16g24110
rosa_chinensis	RchiOBHm_Chr5g0001791 RchiOBHm_Chr7g0210341
rosa_laevigata	RLG00000003045 RLG00000030973
rosa_multiflora	Rmu_co8137394.1_g000001 Rmu_sc0003807.1_g000002 Rmu_ssc0000237.1_g000012
rosa_roxburghii	Rroxscaffold_1G00071250 Rroxscaffold_1G00074540 Rroxscaffold_3G00248380
rosa_rugosa	Rorug04G0393100 Rorug04G0393200 Rorug04G0393300 Rorug07G0121200 Rorug07G0121200 Rorug07G0121300
rosa_samantha	Rh5AG014400 Rh5BG017800 Rh5CG015500 Rh5DG015900 Rh7AG253900 Rh7BG248800 Rh7CG270700 Rh7DG261400
rosa_wichuraiana	Rw5G001390 Rw7G021640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	907
Acc36I	ACCTGC	1 cut(s)	900
AccI	GTMKAC	1 cut(s)	708
AccII	CGCG	1 cut(s)	1015
AciI	CCGC	5 cut(s)	198, 649, 853, 1526, 1678
AclWI	GGATC	5 cut(s)	74, 87, 1065, 1172, 1516
AcoI	YGGCCR	2 cut(s)	199, 1071
AcsI	RAATTY	4 cut(s)	688, 1094, 1628, 1821
AcuI	CTGAAG	2 cut(s)	315, 341
AcyI	GRCGYC	1 cut(s)	904
AdeI	CACNNNGTG	1 cut(s)	195
AfaI	GTAC	7 cut(s)	552, 617, 635, 735, 973, 1276, 1714
AfiI	CCNNNNNNNGG	3 cut(s)	625, 630, 962
AgsI	TTSAA	6 cut(s)	15, 348, 511, 536, 566, 1137
AjnI	CCWGG	3 cut(s)	623, 842, 1504
AjuI	GAANNNNNNNTTGG	2 cut(s)	736, 768
Alw21I	GWGCWC	2 cut(s)	727, 1439
Alw26I	GTCTC	1 cut(s)	592
Alw44I	GTGCAC	1 cut(s)	1435
AlwI	GGATC	5 cut(s)	74, 87, 1065, 1172, 1516
AoxI	GGCC	3 cut(s)	199, 695, 1071
ApaLI	GTGCAC	1 cut(s)	1435
ApeKI	GCWGC	5 cut(s)	87, 388, 646, 1785, 1982
ApoI	RAATTY	4 cut(s)	688, 1094, 1628, 1821
ArsI	GACNNNNNNTTYG	2 cut(s)	889, 921
Asp700I	GAANNNNTTC	3 cut(s)	51, 372, 1742
AspLEI	GCGC	2 cut(s)	356, 1017
AspS9I	GGNCC	2 cut(s)	856, 1969
AsuC2I	CCSGG	1 cut(s)	32
AsuHPI	GGTGA	2 cut(s)	220, 1848
AvaII	GGWCC	2 cut(s)	856, 1969
AxyI	CCTNAGG	1 cut(s)	518
BaeGI	GKGCMC	3 cut(s)	268, 1439, 1605
BamHI	GGATCC	1 cut(s)	79
BanII	GRGCYC	1 cut(s)	25
BbsI	GAAGAC	2 cut(s)	332, 1875
Bbv12I	GWGCWC	2 cut(s)	727, 1439
BbvI	GCAGC	4 cut(s)	99, 400, 658, 1797
BccI	CCATC	2 cut(s)	416, 567
BcgI	CGANNNNNNTGC	4 cut(s)	672, 706, 1865, 1899
BciT130I	CCWGG	3 cut(s)	625, 844, 1506
BciVI	GTATCC	1 cut(s)	1411
BcnI	CCSGG	1 cut(s)	32
BcoDI	GTCTC	1 cut(s)	592
BfaI	CTAG	6 cut(s)	26, 419, 932, 1272, 1452, 1512
BfmI	CTRYAG	3 cut(s)	705, 776, 1018
BfuAI	ACCTGC	1 cut(s)	900
BfuI	GTATCC	1 cut(s)	1411
BisI	GCNGC	6 cut(s)	88, 199, 389, 647, 1786, 1983
BlsI	GCNGC	6 cut(s)	89, 200, 390, 648, 1787, 1984
BmcAI	AGTACT	1 cut(s)	1276
Bme1390I	CCNGG	4 cut(s)	32, 625, 844, 1506
Bme18I	GGWCC	2 cut(s)	856, 1969
BmgT120I	GGNCC	2 cut(s)	856, 1969
BmiI	GGNNCC	4 cut(s)	48, 81, 857, 1960
BmrFI	CCNGG	4 cut(s)	32, 625, 844, 1506
BmrI	ACTGGG	3 cut(s)	27, 805, 1608
BmuI	ACTGGG	3 cut(s)	27, 805, 1608
BoxI	GACNNNNGTC	1 cut(s)	99
BpiI	GAAGAC	2 cut(s)	332, 1875
BpmI	CTGGAG	1 cut(s)	1149
Bpu10I	CCTNAGC	1 cut(s)	236
BpuEI	CTTGAG	1 cut(s)	1258
BpuMI	CCSGG	1 cut(s)	32
BsaHI	GRCGYC	1 cut(s)	904
BsaJI	CCNNGG	2 cut(s)	624, 1164
BsaWI	WCCGGW	1 cut(s)	1403
Bsc4I	CCNNNNNNNGG	3 cut(s)	625, 630, 962
Bse1I	ACTGG	6 cut(s)	22, 312, 720, 811, 1603, 1706
Bse21I	CCTNAGG	1 cut(s)	518
Bse3DI	GCAATG	3 cut(s)	383, 1035, 1521
BseBI	CCWGG	3 cut(s)	625, 844, 1506
BseDI	CCNNGG	2 cut(s)	624, 1164
BseGI	GGATG	3 cut(s)	356, 657, 670
BseLI	CCNNNNNNNGG	3 cut(s)	625, 630, 962
BseMI	GCAATG	3 cut(s)	383, 1035, 1521
BseMII	CTCAG	2 cut(s)	289, 742
BseNI	ACTGG	6 cut(s)	22, 312, 720, 811, 1603, 1706
BseRI	GAGGAG	3 cut(s)	20, 128, 753
BseSI	GKGCMC	3 cut(s)	268, 1439, 1605
BseXI	GCAGC	4 cut(s)	99, 400, 658, 1797
BseYI	CCCAGC	1 cut(s)	927
Bsh1236I	CGCG	1 cut(s)	1015
BshFI	GGCC	3 cut(s)	201, 697, 1073
BsiHKAI	GWGCWC	2 cut(s)	727, 1439
BsiSI	CCGG	2 cut(s)	32, 1404
BslFI	GGGAC	5 cut(s)	14, 541, 780, 953, 1319
BslI	CCNNNNNNNGG	3 cut(s)	625, 630, 962
BsmAI	GTCTC	1 cut(s)	592
BsmBI	CGTCTC	1 cut(s)	592
BsmFI	GGGAC	5 cut(s)	14, 541, 780, 953, 1319
BsmI	GAATGC	1 cut(s)	182
BsnI	GGCC	3 cut(s)	201, 697, 1073
Bsp1286I	GDGCHC	5 cut(s)	25, 268, 727, 1439, 1605
Bsp143I	GATC	6 cut(s)	79, 1057, 1177, 1220, 1508, 1540
BspACI	CCGC	5 cut(s)	198, 649, 853, 1526, 1678
BspANI	GGCC	3 cut(s)	201, 697, 1073
BspCNI	CTCAG	2 cut(s)	288, 741
BspFNI	CGCG	1 cut(s)	1015
BspLI	GGNNCC	4 cut(s)	48, 81, 857, 1960
BspMAI	CTGCAG	1 cut(s)	780
BspMI	ACCTGC	1 cut(s)	900
BspPI	GGATC	5 cut(s)	74, 87, 1065, 1172, 1516
BspQI	GCTCTTC	1 cut(s)	1335
BsrDI	GCAATG	3 cut(s)	383, 1035, 1521
BsrI	ACTGG	6 cut(s)	22, 312, 720, 811, 1603, 1706
BssECI	CCNNGG	2 cut(s)	624, 1164
BssMI	GATC	6 cut(s)	79, 1057, 1177, 1220, 1508, 1540
BssNI	GRCGYC	1 cut(s)	904
Bst2UI	CCWGG	3 cut(s)	625, 844, 1506
Bst4CI	ACNGT	8 cut(s)	555, 620, 949, 1022, 1103, 1300, 1759, 1952
Bst6I	CTCTTC	3 cut(s)	446, 1335, 1913
BstACI	GRCGYC	1 cut(s)	904
BstAPI	GCANNNNNTGC	3 cut(s)	440, 496, 652
BstC8I	GCNNGC	3 cut(s)	164, 196, 1650
BstDEI	CTNAG	6 cut(s)	236, 275, 518, 728, 1046, 1229
BstEII	GGTNACC	1 cut(s)	1854
BstF5I	GGATG	3 cut(s)	356, 657, 670
BstFNI	CGCG	1 cut(s)	1015
BstHHI	GCGC	2 cut(s)	356, 1017
BstKTI	GATC	6 cut(s)	82, 1060, 1180, 1223, 1511, 1543
BstMAI	GTCTC	1 cut(s)	592
BstMBI	GATC	6 cut(s)	79, 1057, 1177, 1220, 1508, 1540
BstMWI	GCNNNNNNNGC	5 cut(s)	204, 440, 496, 652, 797
BstNI	CCWGG	3 cut(s)	625, 844, 1506
BstNSI	RCATGY	3 cut(s)	166, 1592, 1782
BstPAI	GACNNNNGTC	1 cut(s)	99
BstPI	GGTNACC	1 cut(s)	1854
BstSCI	CCNGG	4 cut(s)	30, 623, 842, 1504
BstSFI	CTRYAG	3 cut(s)	705, 776, 1018
BstSLI	GKGCMC	3 cut(s)	268, 1439, 1605
BstUI	CGCG	1 cut(s)	1015
BstV1I	GCAGC	4 cut(s)	99, 400, 658, 1797
BstV2I	GAAGAC	2 cut(s)	332, 1875
BstX2I	RGATCY	3 cut(s)	79, 1057, 1508
BstYI	RGATCY	3 cut(s)	79, 1057, 1508
Bsu36I	CCTNAGG	1 cut(s)	518
BsuI	GTATCC	1 cut(s)	1411
BsuRI	GGCC	3 cut(s)	201, 697, 1073
BtgZI	GCGATG	1 cut(s)	1759
BtsCI	GGATG	3 cut(s)	356, 657, 670
BtsI	GCAGTG	5 cut(s)	42, 97, 1122, 1194, 1583
BtsIMutI	CAGTG	8 cut(s)	42, 97, 727, 1002, 1122, 1194, 1583, 1909
BveI	ACCTGC	1 cut(s)	900
Cac8I	GCNNGC	3 cut(s)	164, 196, 1650
CfoI	GCGC	2 cut(s)	356, 1017
Cfr13I	GGNCC	2 cut(s)	856, 1969
CseI	GACGC	3 cut(s)	188, 609, 1641
Csp6I	GTAC	7 cut(s)	551, 616, 634, 734, 972, 1275, 1713
CviQI	GTAC	7 cut(s)	551, 616, 634, 734, 972, 1275, 1713
DdeI	CTNAG	6 cut(s)	236, 275, 518, 728, 1046, 1229
DpnI	GATC	6 cut(s)	81, 1059, 1179, 1222, 1510, 1542
DpnII	GATC	6 cut(s)	79, 1057, 1177, 1220, 1508, 1540
DraI	TTTAAA	1 cut(s)	1720
DraIII	CACNNNGTG	1 cut(s)	195
EaeI	YGGCCR	2 cut(s)	199, 1071
Eam1104I	CTCTTC	3 cut(s)	446, 1335, 1913
EarI	CTCTTC	3 cut(s)	446, 1335, 1913
Eco24I	GRGCYC	1 cut(s)	25
Eco47I	GGWCC	2 cut(s)	856, 1969
Eco57I	CTGAAG	2 cut(s)	315, 341
Eco81I	CCTNAGG	1 cut(s)	518
Eco91I	GGTNACC	1 cut(s)	1854
EcoO109I	RGGNCCY	1 cut(s)	1969
EcoO65I	GGTNACC	1 cut(s)	1854
EcoRII	CCWGG	3 cut(s)	623, 842, 1504
EcoT22I	ATGCAT	1 cut(s)	865
EcoT38I	GRGCYC	1 cut(s)	25
Esp3I	CGTCTC	1 cut(s)	592
FaqI	GGGAC	5 cut(s)	14, 541, 780, 953, 1319
FauI	CCCGC	1 cut(s)	846
FauNDI	CATATG	1 cut(s)	1484
FblI	GTMKAC	1 cut(s)	708
Fnu4HI	GCNGC	6 cut(s)	88, 199, 389, 647, 1786, 1983
FokI	GGATG	3 cut(s)	343, 664, 677
FriOI	GRGCYC	1 cut(s)	25
Fsp4HI	GCNGC	6 cut(s)	88, 199, 389, 647, 1786, 1983
FspBI	CTAG	6 cut(s)	26, 419, 932, 1272, 1452, 1512
GlaI	GCGC	2 cut(s)	355, 1016
GluI	GCNGC	6 cut(s)	88, 199, 389, 647, 1786, 1983
GsaI	CCCAGC	1 cut(s)	931
GsuI	CTGGAG	1 cut(s)	1149
HaeIII	GGCC	3 cut(s)	201, 697, 1073
HapII	CCGG	2 cut(s)	32, 1404
HgaI	GACGC	3 cut(s)	188, 609, 1641
HhaI	GCGC	2 cut(s)	356, 1017
Hin1I	GRCGYC	1 cut(s)	904
Hin6I	GCGC	2 cut(s)	354, 1015
HinP1I	GCGC	2 cut(s)	354, 1015
HincII	GTYRAC	2 cut(s)	1011, 1580
HindII	GTYRAC	2 cut(s)	1011, 1580
HindIII	AAGCTT	1 cut(s)	314
HinfI	GANTC	4 cut(s)	668, 806, 820, 1576
HpaI	GTTAAC	1 cut(s)	1011
HpaII	CCGG	2 cut(s)	32, 1404
HphI	GGTGA	2 cut(s)	220, 1848
Hpy166II	GTNNAC	8 cut(s)	136, 709, 1011, 1254, 1437, 1580, 1596, 1762
Hpy188I	TCNGA	6 cut(s)	108, 130, 321, 731, 805, 909
Hpy188III	TCNNGA	3 cut(s)	508, 536, 828
Hpy8I	GTNNAC	8 cut(s)	136, 709, 1011, 1254, 1437, 1580, 1596, 1762
Hpy99I	CGWCG	1 cut(s)	906
HpyAV	CCTTC	3 cut(s)	708, 956, 1687
HpyCH4III	ACNGT	8 cut(s)	555, 620, 949, 1022, 1103, 1300, 1759, 1952
HpyCH4IV	ACGT	6 cut(s)	746, 904, 1007, 1308, 1354, 1673
HpyF10VI	GCNNNNNNNGC	5 cut(s)	204, 440, 496, 652, 797
HpyF3I	CTNAG	6 cut(s)	236, 275, 518, 728, 1046, 1229
HpySE526I	ACGT	6 cut(s)	746, 904, 1007, 1308, 1354, 1673
Hsp92I	GRCGYC	1 cut(s)	904
HspAI	GCGC	2 cut(s)	354, 1015
KspAI	GTTAAC	1 cut(s)	1011
Kzo9I	GATC	6 cut(s)	79, 1057, 1177, 1220, 1508, 1540
LguI	GCTCTTC	1 cut(s)	1335
LmnI	GCTCC	1 cut(s)	415
Lsp1109I	GCAGC	4 cut(s)	99, 400, 658, 1797
MaeI	CTAG	6 cut(s)	26, 419, 932, 1272, 1452, 1512
MaeII	ACGT	6 cut(s)	746, 904, 1007, 1308, 1354, 1673
MaeIII	GTNAC	7 cut(s)	92, 248, 887, 1294, 1724, 1834, 1854
MalI	GATC	6 cut(s)	81, 1059, 1179, 1222, 1510, 1542
MboI	GATC	6 cut(s)	79, 1057, 1177, 1220, 1508, 1540
MflI	RGATCY	3 cut(s)	79, 1057, 1508
MhlI	GDGCHC	5 cut(s)	25, 268, 727, 1439, 1605
MlyI	GAGTC	2 cut(s)	800, 1585
MmeI	TCCRAC	1 cut(s)	1895
Mph1103I	ATGCAT	1 cut(s)	865
MroXI	GAANNNNTTC	3 cut(s)	51, 372, 1742
MseI	TTAA	8 cut(s)	543, 794, 1010, 1461, 1565, 1719, 1965, 1987
MslI	CAYNNNNRTG	3 cut(s)	1469, 1557, 1731
MspA1I	CMGCKG	2 cut(s)	649, 1184
MspI	CCGG	2 cut(s)	32, 1404
MspR9I	CCNGG	4 cut(s)	32, 625, 844, 1506
Mva1269I	GAATGC	1 cut(s)	182
MvaI	CCWGG	3 cut(s)	625, 844, 1506
MvnI	CGCG	1 cut(s)	1015
MwoI	GCNNNNNNNGC	5 cut(s)	204, 440, 496, 652, 797
NciI	CCSGG	1 cut(s)	32
NdeI	CATATG	1 cut(s)	1484
NdeII	GATC	6 cut(s)	79, 1057, 1177, 1220, 1508, 1540
NlaIV	GGNNCC	4 cut(s)	48, 81, 857, 1960
NmeAIII	GCCGAG	3 cut(s)	502, 1049, 1297
NmuCI	GTSAC	5 cut(s)	92, 248, 1724, 1834, 1854
NsiI	ATGCAT	1 cut(s)	865
NspI	RCATGY	3 cut(s)	166, 1592, 1782
PaeI	GCATGC	1 cut(s)	166
PciSI	GCTCTTC	1 cut(s)	1335
PctI	GAATGC	1 cut(s)	182
PdmI	GAANNNNTTC	3 cut(s)	51, 372, 1742
PfeI	GAWTC	2 cut(s)	668, 820
PfoI	TCCNGGA	1 cut(s)	1504
PkrI	GCNGC	6 cut(s)	89, 200, 390, 648, 1787, 1984
PleI	GAGTC	2 cut(s)	800, 1584
PpsI	GAGTC	2 cut(s)	800, 1584
PpuMI	RGGWCCY	1 cut(s)	1969
PshAI	GACNNNNGTC	1 cut(s)	99
Psp5II	RGGWCCY	1 cut(s)	1969
Psp6I	CCWGG	3 cut(s)	623, 842, 1504
PspEI	GGTNACC	1 cut(s)	1854
PspFI	CCCAGC	1 cut(s)	927
PspGI	CCWGG	3 cut(s)	623, 842, 1504
PspN4I	GGNNCC	4 cut(s)	48, 81, 857, 1960
PspPI	GGNCC	2 cut(s)	856, 1969
PspPPI	RGGWCCY	1 cut(s)	1969
PstI	CTGCAG	1 cut(s)	780
PsuI	RGATCY	3 cut(s)	79, 1057, 1508
PvuII	CAGCTG	1 cut(s)	1184
RsaI	GTAC	7 cut(s)	552, 617, 635, 735, 973, 1276, 1714
RsaNI	GTAC	7 cut(s)	551, 616, 634, 734, 972, 1275, 1713
RseI	CAYNNNNRTG	3 cut(s)	1469, 1557, 1731
SapI	GCTCTTC	1 cut(s)	1335
SaqAI	TTAA	8 cut(s)	543, 794, 1010, 1461, 1565, 1719, 1965, 1987
SatI	GCNGC	6 cut(s)	88, 199, 389, 647, 1786, 1983
Sau3AI	GATC	6 cut(s)	79, 1057, 1177, 1220, 1508, 1540
Sau96I	GGNCC	2 cut(s)	856, 1969
ScaI	AGTACT	1 cut(s)	1276
SchI	GAGTC	2 cut(s)	800, 1585
ScrFI	CCNGG	4 cut(s)	32, 625, 844, 1506
SduI	GDGCHC	5 cut(s)	25, 268, 727, 1439, 1605
SfcI	CTRYAG	3 cut(s)	705, 776, 1018
SinI	GGWCC	2 cut(s)	856, 1969
SmiMI	CAYNNNNRTG	3 cut(s)	1469, 1557, 1731
SmlI	CTYRAG	1 cut(s)	1237
SmoI	CTYRAG	1 cut(s)	1237
SphI	GCATGC	1 cut(s)	166
SsiI	CCGC	5 cut(s)	198, 649, 853, 1526, 1678
SspI	AATATT	2 cut(s)	495, 1702
SspMI	CTAG	6 cut(s)	26, 419, 932, 1272, 1452, 1512
StyD4I	CCNGG	4 cut(s)	30, 623, 842, 1504
TaaI	ACNGT	8 cut(s)	555, 620, 949, 1022, 1103, 1300, 1759, 1952
TaiI	ACGT	6 cut(s)	749, 907, 1010, 1311, 1357, 1676
TaqI	TCGA	4 cut(s)	870, 1024, 1092, 1391
TatI	WGTACW	2 cut(s)	971, 1274
TauI	GCSGC	1 cut(s)	201
TfiI	GAWTC	2 cut(s)	668, 820
Tru1I	TTAA	8 cut(s)	543, 794, 1010, 1461, 1565, 1719, 1965, 1987
Tru9I	TTAA	8 cut(s)	543, 794, 1010, 1461, 1565, 1719, 1965, 1987
TscAI	CASTG	8 cut(s)	42, 97, 727, 1002, 1129, 1201, 1590, 1909
TseFI	GTSAC	5 cut(s)	92, 248, 1724, 1834, 1854
TseI	GCWGC	5 cut(s)	87, 388, 646, 1785, 1982
Tsp45I	GTSAC	5 cut(s)	92, 248, 1724, 1834, 1854
TspDTI	ATGAA	8 cut(s)	926, 1353, 1362, 1365, 1487, 1782, 1812, 1888
TspGWI	ACGGA	1 cut(s)	125
TspRI	CASTG	8 cut(s)	42, 97, 727, 1002, 1129, 1201, 1590, 1909
VneI	GTGCAC	1 cut(s)	1435
VpaK11BI	GGWCC	2 cut(s)	856, 1969
XapI	RAATTY	4 cut(s)	688, 1094, 1628, 1821
XceI	RCATGY	3 cut(s)	166, 1592, 1782
XcmI	CCANNNNNNNNNTGG	1 cut(s)	304
XmiI	GTMKAC	1 cut(s)	708
XmnI	GAANNNNTTC	3 cut(s)	51, 372, 1742
XspI	CTAG	6 cut(s)	26, 419, 932, 1272, 1452, 1512
ZraI	GACGTC	1 cut(s)	905
ZrmI	AGTACT	1 cut(s)	1276
Zsp2I	ATGCAT	1 cut(s)	865

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.