Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001361

Physical Location & Seq

Genomic Coordinates Reverse (-)

50582661 .. 50585208

Gene Structure 2548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1405100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGCAGTGTGTTACAACACGAGGAGTACTATCAGTAGCAAGAAAAAGGCGTGTCCATTCGCAGTTTAATGATCTACAAGACTGCTACTTGCAAAAGCGACGAAATTGGAACAGACAGGAAGAGGACACAAACGCCATGGACATAGAAGGTTACAATCCAGGCCTTGAAGATTTTCAATCTGTGCTTGCAAGCTTTACACAATACAGTCGATTGAGAGTCGTTGCTGAACTGAGTCACGGTGATCTTTTTCACTCTGCTAATATTGTGTCAAGTATAGAATTTGATCGTGATGAAGAGCTCTTTGCTACTGCTGGAGTTTCACGGTGCATCAAGGTTTTTGAATTTTCCTCGGTGGTTAACGAACCAACCGATATCCACTGTCCTATTGTGGAGATATCCTCACGATCCAAACTTAGTTGCTTGAGCTGGAATAAGTACACTAGAAACCATATTGCTAGTAGTGACTACGAGGGGAGTATGATGGAATATGAGGAGCATGAAAAGCGAGCTTGGAGCGTTGATTTTTCATGCACCGATCCTTCAATGCTTGTGTCCGGAAGTGATGACTGCAAGGTCAAAATTTGGTGCACAAAGCAAGAACCAAGTGTTCTTAACATCGACATGAAGGCAAATATATGTTCTGTCAAGTATAATCCTGGATCCAGCTTTTTCGTGGCAGTTGGCTCTGCTGACCATCACATTCATTATTATGACTTGAGAAACATCAGCCAACCACTGCACGTGTTCCGTGGGCATAGAAAAGCTGTTTCGTATGTAAAATTCTTGTCTAACAACGAACTTGCTTCTGCATCCACTGACAGCACACTGCGGTTGTGGGACGTAAAGGAAAACTTGCCCTTGTGCATGTACAGAGGCCACATGAACGAGAAGAACTTTGTAGGTCTTGCAGTAAATAATGAATACATTGCTTGTGGCAGCGAGACAAATGAACTGTTCGTATACCATAAGGCCATCTCGAAACCTATGACTTGGCACAGATTTGGCTCTTCGGATTTAGGCGATAATGACGACGATCCAGGACCACACTTTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0000152 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009639 GO:0009640 GO:0009641 GO:0009642 GO:0009646 GO:0009647 GO:0009648 GO:0009649 GO:0009791 GO:0009812 GO:0009987 GO:0010119 GO:0010228 GO:0016567 GO:0016604 GO:0016740 GO:0019538 GO:0019787 GO:0022414 GO:0031461 GO:0031974 GO:0031981 GO:0032446 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0036211 GO:0042440 GO:0042752 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046283 GO:0046483 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0061630 GO:0061659 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080008 GO:0090304 GO:0140096 GO:1901360 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

WD40 zf-C3HC4_2

Protein Analysis

352

Amino Acids

40.08

Weight (kDa)

5.83

Isoelectric Point (pI)

53.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	86 - 322	1.3e-25	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	88 - 238	5.7e-06	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	138 - 241	5.3e-11	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	160 - 279	3.8e-12	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	171 - 264	4e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML_2	PF23414	171 - 327	4.8e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	177 - 308	4.4e-11	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	179 - 287	2.9e-09	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	181 - 291	2.5e-11	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	207 - 313	2.3e-14	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	209 - 317	8.1e-12	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	209 - 284	7.7e-10	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	214 - 329	4.8e-08	CDC20/Fizzy WD40 domain
WD40	PF00400	244 - 280	7.2e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	248 - 319	5.8e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32950 AT2G32950
fragaria_vesca	FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_3g01260 FvH4_5g22570 FvH4_5g22570
malus_domestica	MD06G1037700.v1.1 MD10G1328500.v1.1 MD15G1405100.v1.1 MD15G1405200.v1.1 MD16G1272200.v1.1
prunus_persica	Prupe.4G013500_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1 Prupe.5G031300_v2.0.a1
pyrus_communis	pycom10g27830 pycom16g24110
rosa_chinensis	RchiOBHm_Chr5g0001791 RchiOBHm_Chr7g0210341
rosa_laevigata	RLG00000003045 RLG00000030973
rosa_multiflora	Rmu_co8137394.1_g000001 Rmu_sc0003807.1_g000002 Rmu_ssc0000237.1_g000012
rosa_roxburghii	Rroxscaffold_1G00071250 Rroxscaffold_1G00074540 Rroxscaffold_3G00248380
rosa_rugosa	Rorug04G0393100 Rorug04G0393200 Rorug04G0393300 Rorug07G0121200 Rorug07G0121200 Rorug07G0121300
rosa_samantha	Rh5AG014400 Rh5BG017800 Rh5CG015500 Rh5DG015900 Rh7AG253900 Rh7BG248800 Rh7CG270700 Rh7DG261400
rosa_wichuraiana	Rw5G001390 Rw7G021640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	572
AccI	GTMKAC	1 cut(s)	960
AccIII	TCCGGA	1 cut(s)	554
AciI	CCGC	1 cut(s)	829
AclWI	GGATC	5 cut(s)	399, 530, 654, 667, 1028
AcsI	RAATTY	4 cut(s)	278, 341, 579, 779
AcvI	CACGTG	1 cut(s)	742
AfaI	GTAC	3 cut(s)	27, 437, 869
AflIII	ACRYGT	1 cut(s)	741
AgsI	TTSAA	4 cut(s)	167, 176, 341, 543
AjnI	CCWGG	3 cut(s)	157, 655, 1036
AluBI	AGCT	6 cut(s)	192, 298, 426, 509, 666, 764
AluI	AGCT	6 cut(s)	192, 298, 426, 509, 666, 764
Alw21I	GWGCWC	2 cut(s)	300, 590
Alw26I	GTCTC	1 cut(s)	935
Alw44I	GTGCAC	1 cut(s)	586
AlwI	GGATC	5 cut(s)	399, 530, 654, 667, 1028
Aor13HI	TCCGGA	1 cut(s)	554
AoxI	GGCC	3 cut(s)	160, 874, 969
ApaLI	GTGCAC	1 cut(s)	586
ApeKI	GCWGC	1 cut(s)	936
ApoI	RAATTY	4 cut(s)	278, 341, 579, 779
Asp700I	GAANNNNTTC	1 cut(s)	171
AspS9I	GGNCC	1 cut(s)	1040
AsuHPI	GGTGA	1 cut(s)	251
AvaII	GGWCC	1 cut(s)	1040
BaeGI	GKGCMC	1 cut(s)	590
BamHI	GGATCC	1 cut(s)	659
BanII	GRGCYC	1 cut(s)	300
BauI	CACGAG	1 cut(s)	18
BbrPI	CACGTG	1 cut(s)	742
Bbv12I	GWGCWC	2 cut(s)	300, 590
BbvI	GCAGC	1 cut(s)	948
BccI	CCATC	3 cut(s)	475, 702, 980
BciT130I	CCWGG	3 cut(s)	159, 657, 1038
BcoDI	GTCTC	1 cut(s)	935
BfaI	CTAG	2 cut(s)	441, 456
BisI	GCNGC	1 cut(s)	937
BlsI	GCNGC	1 cut(s)	938
BmcAI	AGTACT	1 cut(s)	27
Bme1390I	CCNGG	3 cut(s)	159, 657, 1038
Bme18I	GGWCC	1 cut(s)	1040
BmgT120I	GGNCC	1 cut(s)	1040
BmiI	GGNNCC	1 cut(s)	661
BmrFI	CCNGG	3 cut(s)	159, 657, 1038
BmsI	GCATC	2 cut(s)	336, 818
BplI	GAGNNNNNCTC	2 cut(s)	383, 415
BpmI	CTGGAG	1 cut(s)	333
BpuEI	CTTGAG	2 cut(s)	442, 736
BsaAI	YACGTR	1 cut(s)	742
BsaJI	CCNNGG	3 cut(s)	135, 348, 748
BsaWI	WCCGGW	1 cut(s)	554
Bse3DI	GCAATG	1 cut(s)	924
BseAI	TCCGGA	1 cut(s)	554
BseBI	CCWGG	3 cut(s)	159, 657, 1038
BseDI	CCNNGG	3 cut(s)	135, 348, 748
BseGI	GGATG	1 cut(s)	809
BseMI	GCAATG	1 cut(s)	924
BseMII	CTCAG	1 cut(s)	221
BseRI	GAGGAG	2 cut(s)	36, 506
BseSI	GKGCMC	1 cut(s)	590
BseXI	GCAGC	1 cut(s)	948
BsgI	GTGCAG	1 cut(s)	722
BshFI	GGCC	3 cut(s)	162, 876, 971
BsiHKAI	GWGCWC	2 cut(s)	300, 590
BsiSI	CCGG	1 cut(s)	555
BslFI	GGGAC	1 cut(s)	851
BsmAI	GTCTC	1 cut(s)	935
BsmFI	GGGAC	1 cut(s)	851
BsnI	GGCC	3 cut(s)	162, 876, 971
Bsp1286I	GDGCHC	2 cut(s)	300, 590
Bsp13I	TCCGGA	1 cut(s)	554
Bsp1407I	TGTACA	1 cut(s)	867
Bsp143I	GATC	7 cut(s)	70, 241, 283, 404, 535, 659, 1033
Bsp19I	CCATGG	1 cut(s)	135
BspACI	CCGC	1 cut(s)	829
BspANI	GGCC	3 cut(s)	162, 876, 971
BspCNI	CTCAG	1 cut(s)	222
BspEI	TCCGGA	1 cut(s)	554
BspLI	GGNNCC	1 cut(s)	661
BspPI	GGATC	5 cut(s)	399, 530, 654, 667, 1028
BspQI	GCTCTTC	2 cut(s)	288, 1012
BsrDI	GCAATG	1 cut(s)	924
BsrGI	TGTACA	1 cut(s)	867
BssECI	CCNNGG	3 cut(s)	135, 348, 748
BssMI	GATC	7 cut(s)	70, 241, 283, 404, 535, 659, 1033
BssNAI	GTATAC	1 cut(s)	961
BssSI	CACGAG	1 cut(s)	18
BssT1I	CCWWGG	1 cut(s)	135
Bst1107I	GTATAC	1 cut(s)	961
Bst2BI	CACGAG	1 cut(s)	18
Bst2UI	CCWGG	3 cut(s)	159, 657, 1038
Bst4CI	ACNGT	5 cut(s)	206, 239, 324, 380, 954
Bst6I	CTCTTC	3 cut(s)	114, 288, 1012
BstAUI	TGTACA	1 cut(s)	867
BstBAI	YACGTR	1 cut(s)	742
BstC8I	GCNNGC	3 cut(s)	186, 190, 507
BstDEI	CTNAG	2 cut(s)	230, 413
BstDSI	CCRYGG	2 cut(s)	135, 748
BstF5I	GGATG	1 cut(s)	809
BstKTI	GATC	7 cut(s)	73, 244, 286, 407, 538, 662, 1036
BstMAI	GTCTC	1 cut(s)	935
BstMBI	GATC	7 cut(s)	70, 241, 283, 404, 535, 659, 1033
BstMWI	GCNNNNNNNGC	1 cut(s)	502
BstNI	CCWGG	3 cut(s)	159, 657, 1038
BstNSI	RCATGY	1 cut(s)	868
BstSCI	CCNGG	3 cut(s)	157, 655, 1036
BstSLI	GKGCMC	1 cut(s)	590
BstV1I	GCAGC	1 cut(s)	948
BstX2I	RGATCY	1 cut(s)	659
BstYI	RGATCY	1 cut(s)	659
BstZ17I	GTATAC	1 cut(s)	961
BsuRI	GGCC	3 cut(s)	162, 876, 971
BtgI	CCRYGG	2 cut(s)	135, 748
BtsCI	GGATG	1 cut(s)	809
BtsI	GCAGTG	3 cut(s)	11, 734, 824
BtsIMutI	CAGTG	5 cut(s)	11, 376, 734, 813, 824
Cac8I	GCNNGC	3 cut(s)	186, 190, 507
Cfr13I	GGNCC	1 cut(s)	1040
Csp6I	GTAC	3 cut(s)	26, 436, 868
CviAII	CATG	6 cut(s)	136, 497, 528, 622, 865, 880
CviQI	GTAC	3 cut(s)	26, 436, 868
DdeI	CTNAG	2 cut(s)	230, 413
DpnI	GATC	7 cut(s)	72, 243, 285, 406, 537, 661, 1035
DpnII	GATC	7 cut(s)	70, 241, 283, 404, 535, 659, 1033
DrdI	GACNNNNNNGTC	1 cut(s)	572
DseDI	GACNNNNNNGTC	1 cut(s)	572
Eam1104I	CTCTTC	3 cut(s)	114, 288, 1012
EarI	CTCTTC	3 cut(s)	114, 288, 1012
Ecl136II	GAGCTC	1 cut(s)	298
Eco130I	CCWWGG	1 cut(s)	135
Eco147I	AGGCCT	1 cut(s)	162
Eco24I	GRGCYC	1 cut(s)	300
Eco32I	GATATC	2 cut(s)	373, 396
Eco47I	GGWCC	1 cut(s)	1040
Eco53kI	GAGCTC	1 cut(s)	298
Eco72I	CACGTG	1 cut(s)	742
EcoICRI	GAGCTC	1 cut(s)	298
EcoRII	CCWGG	3 cut(s)	157, 655, 1036
EcoRV	GATATC	2 cut(s)	373, 396
EcoT14I	CCWWGG	1 cut(s)	135
EcoT38I	GRGCYC	1 cut(s)	300
ErhI	CCWWGG	1 cut(s)	135
FaeI	CATG	6 cut(s)	139, 500, 531, 625, 868, 883
FalI	AAGNNNNNCTT	2 cut(s)	836, 868
FaqI	GGGAC	1 cut(s)	851
FatI	CATG	6 cut(s)	135, 496, 527, 621, 864, 879
FblI	GTMKAC	1 cut(s)	960
Fnu4HI	GCNGC	1 cut(s)	937
FokI	GGATG	1 cut(s)	796
FriOI	GRGCYC	1 cut(s)	300
Fsp4HI	GCNGC	1 cut(s)	937
FspBI	CTAG	2 cut(s)	441, 456
GluI	GCNGC	1 cut(s)	937
GsuI	CTGGAG	1 cut(s)	333
HaeIII	GGCC	3 cut(s)	162, 876, 971
HapII	CCGG	1 cut(s)	555
Hin1II	CATG	6 cut(s)	139, 500, 531, 625, 868, 883
HincII	GTYRAC	1 cut(s)	358
HindII	GTYRAC	1 cut(s)	358
HindIII	AAGCTT	1 cut(s)	190
HinfI	GANTC	2 cut(s)	216, 232
HpaI	GTTAAC	1 cut(s)	358
HpaII	CCGG	1 cut(s)	555
HphI	GGTGA	1 cut(s)	251
Hpy166II	GTNNAC	4 cut(s)	358, 438, 588, 961
Hpy188I	TCNGA	1 cut(s)	1012
Hpy188III	TCNNGA	4 cut(s)	287, 402, 555, 976
Hpy8I	GTNNAC	4 cut(s)	358, 438, 588, 961
Hpy99I	CGWCG	2 cut(s)	102, 1034
HpyAV	CCTTC	3 cut(s)	140, 549, 619
HpyCH4III	ACNGT	5 cut(s)	206, 239, 324, 380, 954
HpyCH4IV	ACGT	2 cut(s)	741, 840
HpyF10VI	GCNNNNNNNGC	1 cut(s)	502
HpyF3I	CTNAG	2 cut(s)	230, 413
HpySE526I	ACGT	2 cut(s)	741, 840
Hsp92II	CATG	6 cut(s)	139, 500, 531, 625, 868, 883
Kpn2I	TCCGGA	1 cut(s)	554
KspAI	GTTAAC	1 cut(s)	358
Kzo9I	GATC	7 cut(s)	70, 241, 283, 404, 535, 659, 1033
LguI	GCTCTTC	2 cut(s)	288, 1012
LmnI	GCTCC	2 cut(s)	493, 513
Lsp1109I	GCAGC	1 cut(s)	948
LweI	GCATC	2 cut(s)	336, 818
MaeI	CTAG	2 cut(s)	441, 456
MaeII	ACGT	2 cut(s)	741, 840
MaeIII	GTNAC	4 cut(s)	10, 149, 233, 461
MalI	GATC	7 cut(s)	72, 243, 285, 406, 537, 661, 1035
MboI	GATC	7 cut(s)	70, 241, 283, 404, 535, 659, 1033
MboII	GAAGA	5 cut(s)	131, 179, 305, 901, 999
MflI	RGATCY	1 cut(s)	659
MhlI	GDGCHC	2 cut(s)	300, 590
MluCI	AATT	5 cut(s)	103, 278, 341, 579, 779
MlyI	GAGTC	2 cut(s)	225, 241
MnlI	CCTC	7 cut(s)	14, 115, 358, 409, 463, 484, 866
MroI	TCCGGA	1 cut(s)	554
MroXI	GAANNNNTTC	1 cut(s)	171
MseI	TTAA	3 cut(s)	66, 357, 612
MslI	CAYNNNNRTG	2 cut(s)	620, 708
MspI	CCGG	1 cut(s)	555
MspR9I	CCNGG	3 cut(s)	159, 657, 1038
MvaI	CCWGG	3 cut(s)	159, 657, 1038
MwoI	GCNNNNNNNGC	1 cut(s)	502
NcoI	CCATGG	1 cut(s)	135
NdeII	GATC	7 cut(s)	70, 241, 283, 404, 535, 659, 1033
NlaIII	CATG	6 cut(s)	139, 500, 531, 625, 868, 883
NlaIV	GGNNCC	1 cut(s)	661
NmuCI	GTSAC	2 cut(s)	233, 461
NspI	RCATGY	1 cut(s)	868
PceI	AGGCCT	1 cut(s)	162
PciSI	GCTCTTC	2 cut(s)	288, 1012
PcsI	WCGNNNNNNNCGW	1 cut(s)	366
PdmI	GAANNNNTTC	1 cut(s)	171
PfoI	TCCNGGA	2 cut(s)	655, 1036
PkrI	GCNGC	1 cut(s)	938
PleI	GAGTC	2 cut(s)	224, 240
PmaCI	CACGTG	1 cut(s)	742
PmlI	CACGTG	1 cut(s)	742
PpsI	GAGTC	2 cut(s)	224, 240
Ppu21I	YACGTR	1 cut(s)	742
Psp124BI	GAGCTC	1 cut(s)	300
Psp6I	CCWGG	3 cut(s)	157, 655, 1036
PspCI	CACGTG	1 cut(s)	742
PspGI	CCWGG	3 cut(s)	157, 655, 1036
PspN4I	GGNNCC	1 cut(s)	661
PspPI	GGNCC	1 cut(s)	1040
PsuI	RGATCY	1 cut(s)	659
RsaI	GTAC	3 cut(s)	27, 437, 869
RsaNI	GTAC	3 cut(s)	26, 436, 868
RseI	CAYNNNNRTG	2 cut(s)	620, 708
SacI	GAGCTC	1 cut(s)	300
SapI	GCTCTTC	2 cut(s)	288, 1012
SaqAI	TTAA	3 cut(s)	66, 357, 612
SatI	GCNGC	1 cut(s)	937
Sau3AI	GATC	7 cut(s)	70, 241, 283, 404, 535, 659, 1033
Sau96I	GGNCC	1 cut(s)	1040
ScaI	AGTACT	1 cut(s)	27
SchI	GAGTC	2 cut(s)	225, 241
ScrFI	CCNGG	3 cut(s)	159, 657, 1038
SduI	GDGCHC	2 cut(s)	300, 590
SfaNI	GCATC	2 cut(s)	336, 818
SinI	GGWCC	1 cut(s)	1040
SmiMI	CAYNNNNRTG	2 cut(s)	620, 708
SmlI	CTYRAG	2 cut(s)	421, 715
SmoI	CTYRAG	2 cut(s)	421, 715
Sse9I	AATT	5 cut(s)	103, 278, 341, 579, 779
SseBI	AGGCCT	1 cut(s)	162
SsiI	CCGC	1 cut(s)	829
SspI	AATATT	1 cut(s)	262
SspMI	CTAG	2 cut(s)	441, 456
SstI	GAGCTC	1 cut(s)	300
StuI	AGGCCT	1 cut(s)	162
StyD4I	CCNGG	3 cut(s)	157, 655, 1036
StyI	CCWWGG	1 cut(s)	135
TaaI	ACNGT	5 cut(s)	206, 239, 324, 380, 954
TaiI	ACGT	2 cut(s)	744, 843
TaqI	TCGA	3 cut(s)	208, 618, 977
TasI	AATT	5 cut(s)	103, 278, 341, 579, 779
TatI	WGTACW	3 cut(s)	25, 435, 867
Tru1I	TTAA	3 cut(s)	66, 357, 612
Tru9I	TTAA	3 cut(s)	66, 357, 612
TscAI	CASTG	4 cut(s)	383, 741, 820, 831
TseFI	GTSAC	2 cut(s)	233, 461
TseI	GCWGC	1 cut(s)	936
Tsp45I	GTSAC	2 cut(s)	233, 461
TspDTI	ATGAA	8 cut(s)	306, 513, 516, 638, 692, 896, 933, 963
TspGWI	ACGGA	1 cut(s)	737
TspRI	CASTG	4 cut(s)	383, 741, 820, 831
VneI	GTGCAC	1 cut(s)	586
VpaK11BI	GGWCC	1 cut(s)	1040
XapI	RAATTY	4 cut(s)	278, 341, 579, 779
XceI	RCATGY	1 cut(s)	868
XcmI	CCANNNNNNNNNTGG	1 cut(s)	670
XmiI	GTMKAC	1 cut(s)	960
XmnI	GAANNNNTTC	1 cut(s)	171
XspI	CTAG	2 cut(s)	441, 456
ZrmI	AGTACT	1 cut(s)	27

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.